NEU3

Summary

Gene Symbol: NEU3
Description: neuraminidase 3
Alias: SIAL3, sialidase-3, N-acetyl-alpha-neuraminidase 3, ganglioside sialidase, membrane sialidase, sialidase 3 (membrane sialidase)
Species: human
Products:     NEU3

Top Publications

  1. Kakugawa Y, Wada T, Yamaguchi K, Yamanami H, Ouchi K, Sato I, et al. Up-regulation of plasma membrane-associated ganglioside sialidase (Neu3) in human colon cancer and its involvement in apoptosis suppression. Proc Natl Acad Sci U S A. 2002;99:10718-23 pubmed
    Human plasma membrane-associated sialidase (Neu3) is unique in specifically hydrolyzing gangliosides, thought to participate in cell differentiation and transmembrane signaling, thereby playing crucial roles in the regulation of cell ..
  2. Kawamura S, Sato I, Wada T, Yamaguchi K, Li Y, Li D, et al. Plasma membrane-associated sialidase (NEU3) regulates progression of prostate cancer to androgen-independent growth through modulation of androgen receptor signaling. Cell Death Differ. 2012;19:170-9 pubmed publisher
    ..treatments for androgen-independent prostate cancer, we have investigated plasma membrane-associated sialidase (NEU3), the key enzyme for ganglioside hydrolysis participating in transmembrane signaling...
  3. Albohy A, Li M, Zheng R, Zou C, Cairo C. Insight into substrate recognition and catalysis by the human neuraminidase 3 (NEU3) through molecular modeling and site-directed mutagenesis. Glycobiology. 2010;20:1127-38 pubmed publisher
    ..Members of the family, such as NEU2 and NEU1, are cytosolic or lysosomal, while NEU3 and NEU4 are membrane-associated...
  4. Ueno S, Saito S, Wada T, Yamaguchi K, Satoh M, Arai Y, et al. Plasma membrane-associated sialidase is up-regulated in renal cell carcinoma and promotes interleukin-6-induced apoptosis suppression and cell motility. J Biol Chem. 2006;281:7756-64 pubmed
    Human plasma membrane-associated sialidase (NEU3), specifically hydrolyzing gangliosides, plays crucial roles in the regulation of cell surface functions...
  5. Wada T, Yoshikawa Y, Tokuyama S, Kuwabara M, Akita H, Miyagi T. Cloning, expression, and chromosomal mapping of a human ganglioside sialidase. Biochem Biophys Res Commun. 1999;261:21-7 pubmed
    Here we report the cDNA sequence of a human ganglioside sialidase. The cDNA was isolated from a human brain cDNA library by screening with a 240 bp probe generated by polymerase chain reaction using primers based on the sequences of rat ..
  6. Wang Y, Yamaguchi K, Wada T, Hata K, Zhao X, Fujimoto T, et al. A close association of the ganglioside-specific sialidase Neu3 with caveolin in membrane microdomains. J Biol Chem. 2002;277:26252-9 pubmed
    The ganglioside-specific sialidase Neu3 has been suggested to play essential roles in regulation of cell surface functions because of its major localization in the plasma membrane and strict substrate preference for gangliosides involved ..
  7. Yamaguchi K, Koseki K, Shiozaki M, Shimada Y, Wada T, Miyagi T. Regulation of plasma-membrane-associated sialidase NEU3 gene by Sp1/Sp3 transcription factors. Biochem J. 2010;430:107-17 pubmed publisher
    Gene expression of the human plasma membrane-associated sialidase (NEU3), a key enzyme for ganglioside degradation, is relatively high in brain and is modulated in response to many cellular processes, including neuronal cell ..
  8. Kato K, Shiga K, Yamaguchi K, Hata K, Kobayashi T, Miyazaki K, et al. Plasma-membrane-associated sialidase (NEU3) differentially regulates integrin-mediated cell proliferation through laminin- and fibronectin-derived signalling. Biochem J. 2006;394:647-56 pubmed
    We have found previously that human plasma-membrane-associated sialidase (NEU3), a key glycosidase for ganglioside degradation, was markedly up-regulated in human colon cancers, with an involvement in suppression of apoptosis...
  9. Wada T, Hata K, Yamaguchi K, Shiozaki K, Koseki K, Moriya S, et al. A crucial role of plasma membrane-associated sialidase in the survival of human cancer cells. Oncogene. 2007;26:2483-90 pubmed
    Human plasma membrane-associated sialidase (NEU3), a key enzyme for ganglioside degradation, is markedly upregulated in human cancers, leading to apoptosis suppression...

More Information

Publications76

  1. Bonardi D, Papini N, Pasini M, Dileo L, Orizio F, Monti E, et al. Sialidase NEU3 dynamically associates to different membrane domains specifically modifying their ganglioside pattern and triggering Akt phosphorylation. PLoS ONE. 2014;9:e99405 pubmed publisher
    ..Sialidase NEU3 shows high enzymatic specificity toward gangliosides...
  2. Yang W, Heithoff D, Aziz P, Haslund Gourley B, Westman J, Narisawa S, et al. Accelerated Aging and Clearance of Host Anti-inflammatory Enzymes by Discrete Pathogens Fuels Sepsis. Cell Host Microbe. 2018;24:500-513.e5 pubmed publisher
    ..coli through activation of host Toll-like receptor 4, which triggered Neu1 and Neu3 neuraminidase induction...
  3. Orizio F, Triggiani L, Colosini A, Buglione M, Pasinetti N, Monti E, et al. Overexpression of sialidase NEU3 increases the cellular radioresistance potential of U87MG glioblastoma cells. Biochem Biophys Res Commun. 2019;508:31-36 pubmed publisher
    The plasma membrane-associated sialidase NEU3 is known to play important roles in different physiological and pathophysiological processes such as proliferation, cellular differentiation and tumorigenesis...
  4. Glanz V, Myasoedova V, Grechko A, Orekhov A. Sialidase activity in human pathologies. Eur J Pharmacol. 2019;842:345-350 pubmed publisher
    ..Four types of mammalian sialidases have been described: NEU1, NEU2, NEU3 and NEU4. They are encoded by different genes and characterized by different subcellular localization...
  5. Balatti V, Oghumu S, Bottoni A, Maharry K, Cascione L, Fadda P, et al. MicroRNA Profiling of Salivary Duct Carcinoma Versus Her2/Neu Overexpressing Breast Carcinoma Identify miR-10a as a Putative Breast Related Oncogene. Head Neck Pathol. 2018;: pubmed publisher
    Salivary duct carcinomas (SDC) and Her2/Neu3-overexpressing invasive breast carcinomas (HNPIBC/IBC) are histologically indistinguishable...
  6. Finlay T, Jayanth P, Amith S, Gilmour A, Guzzo C, Gee K, et al. Thymoquinone from nutraceutical black cumin oil activates Neu4 sialidase in live macrophage, dendritic, and normal and type I sialidosis human fibroblast cells via GPCR Galphai proteins and matrix metalloproteinase-9. Glycoconj J. 2010;27:329-48 pubmed publisher
    ..RT-PCR and western blot analyses reveal no correlation between mRNA and protein values for Neu3 and Neu4 in human monocytic THP-1 cells, suggesting for the first time a varied post-transcriptional mechanism for ..
  7. Chigwechokha P, Komatsu M, Itakura T, Shiozaki K. Nile Tilapia Neu3 sialidases: molecular cloning, functional characterization and expression in Oreochromis niloticus. Gene. 2014;552:155-64 pubmed publisher
    Mammalian Neu3 is a ganglioside specific sialidase. Gangliosides are involved in various physiological events such as cell growth, differentiation and diseases...
  8. Pshezhetsky A, Ashmarina M. Keeping it trim: roles of neuraminidases in CNS function. Glycoconj J. 2018;35:375-386 pubmed publisher
    ..Emerging data demonstrate that these enzymes with different, yet overlapping expression patterns, intracellular localization and substrate specificity play essential roles in the physiology of the CNS. ..
  9. Honda A, Chigwechokha P, Kamada Futagami Y, Komatsu M, Shiozaki K. Unique nuclear localization of Nile tilapia (Oreochromis niloticus) Neu4 sialidase is regulated by nuclear transport receptor importin ?/?. Biochimie. 2018;149:92-104 pubmed publisher
    Sialidase catalyzes the removal of sialic acids from glycoconjugates. Different from Neu1 and Neu3 sialidases, Neu4 enzymatic properties such as substrate specificity and subcellular localization are not well-conserved among vertebrates...
  10. Albohy A, Richards M, Cairo C. Mapping substrate interactions of the human membrane-associated neuraminidase, NEU3, using STD NMR. Glycobiology. 2015;25:284-93 pubmed publisher
    ..We chose to employ STD NMR in our ongoing investigations of the human membrane-associated neuraminidase NEU3 and its interaction with glycolipid substrates (e.g., GM3)...
  11. Shiozaki K, Takahashi K, Hosono M, Yamaguchi K, Hata K, Shiozaki M, et al. Phosphatidic acid-mediated activation and translocation to the cell surface of sialidase NEU3, promoting signaling for cell migration. FASEB J. 2015;29:2099-111 pubmed publisher
    The plasma membrane-associated sialidase NEU3 plays crucial roles in regulation of transmembrane signaling, and its aberrant up-regulation in various cancers contributes to malignancy...
  12. Miyagi T, Takahashi K, Yamamoto K, Shiozaki K, Yamaguchi K. Biological and Pathological Roles of Ganglioside Sialidases. Prog Mol Biol Transl Sci. 2018;156:121-150 pubmed publisher
    ..In mammals, there are four forms of sialidase (Neu1, Neu2, Neu3, and Neu4), differing in their major subcellular localization and enzymatic properties...
  13. Richards M, Guo T, Hunter C, Cairo C. Molecular dynamics simulations of viral neuraminidase inhibitors with the human neuraminidase enzymes: Insights into isoenzyme selectivity. Bioorg Med Chem. 2018;26:5349-5358 pubmed publisher
    ..Humans can express multiple forms of neuraminidase enzymes (NEU1, NEU2, NEU3, NEU4) that share a similar active site and enzymatic mechanism with their viral counterparts...
  14. Yang X, Li S, Xiang S, Wu Y, Wang L, Peng J, et al. [Effect of attenuated expression of neuraminidase 3 via RNA interference on the proliferation and apoptosis of osteosarcoma MG-63 cells]. Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2018;32:887-892 pubmed publisher
    To investigate the effect of attenuated expression of neuraminidase 3 (NEU3) via RNA interference on the proliferation and apoptosis in human osteosarcoma MG-63 cells. MG-63 cells were immunostained to observe the expression of NEU3...
  15. Ha T, Kim Y, Lee G. Understanding of altered N-glycosylation-related gene expression in recombinant Chinese hamster ovary cells subjected to elevated ammonium concentration by digital mRNA counting. Biotechnol Bioeng. 2015;112:1583-93 pubmed publisher
    ..genes, thirteen genes (gale, nans, gpi, man2a1, b4galt5, b4galt7, st3gal2, st3gal5, glb1, hexa, hexb, neu1, and neu3) were up-regulated over 1...
  16. Hakomori S, Handa K. GM3 and cancer. Glycoconj J. 2015;32:1-8 pubmed publisher
    ..The important role of the sialosyl group of GM3 was demonstrated using NEU3, a plasma membrane-associated sialidase that selectively remove sialic acids from gangliosides GM3 and GD1a and is ..
  17. Pan X, De Aragão C, Velasco Martin J, Priestman D, Wu H, Takahashi K, et al. Neuraminidases 3 and 4 regulate neuronal function by catabolizing brain gangliosides. FASEB J. 2017;31:3467-3483 pubmed publisher
    ..Wu, H. Y., Takahashi, K., Yamaguchi, K., Sturiale, L., Garozzo, D., Platt, F. M., Lamarche-Vane, N., Morales, C. R., Miyagi, T., Pshezhetsky, A. V. Neuraminidases 3 and 4 regulate neuronal function by catabolizing brain gangliosides. ..
  18. Yang W, Aziz P, Heithoff D, Mahan M, Smith J, Marth J. An intrinsic mechanism of secreted protein aging and turnover. Proc Natl Acad Sci U S A. 2015;112:13657-62 pubmed publisher
    ..Endogenous glycosidases, including neuraminidase 1 (Neu1), neuraminidase 3 (Neu3), beta-galactosidase 1 (Glb1), and hexosaminidase B (HexB), possess hydrolytic activities that temporally remodel N-..
  19. Higuero A, Díez Revuelta N, Abad Rodriguez J. The sugar code in neuronal physiology. Histochem Cell Biol. 2017;147:257-267 pubmed publisher
    ..Among others, glycan-related proteins as sialidase Neu3 or galectins-1, -3, and -4 play central roles in the determination of axonal fate, axon growth, guidance and ..
  20. Feng C, Li J, Snyder G, Huang W, Goldblum S, Chen W, et al. Antibody against Microbial Neuraminidases Recognizes Human Sialidase 3 (NEU3): the Neuraminidase/Sialidase Superfamily Revisited. MBio. 2017;8: pubmed publisher
    ..perfringens NA, as well as anti-influenza virus NA serum, recognize human NEU3 but not NEU1 and that antibodies to C. perfringens NA inhibit NEU3 enzymatic activity...
  21. Minami A, Saito M, Mamada S, Ieno D, Hikita T, Takahashi T, et al. Role of Sialidase in Long-Term Potentiation at Mossy Fiber-CA3 Synapses and Hippocampus-Dependent Spatial Memory. PLoS ONE. 2016;11:e0165257 pubmed publisher
    ..Sialidase (EC 3.2.1.18), which has 4 isozymes including Neu1, Neu2, Neu3 and Neu4, regulates the sialylation level of glycans by removing sialic acid from sialylglycoconjugate...
  22. Lipina C, Nardi F, GRACE H, Hundal H. NEU3 sialidase as a marker of insulin sensitivity: Regulation by fatty acids. Cell Signal. 2015;27:1742-50 pubmed publisher
    The plasma membrane-associated enzyme NEU3 sialidase functions to cleave sialic acid residues from the ganglioside GM3 thereby promoting its degradation, and has been implicated in the modulation of insulin action...
  23. Rodriguez Walker M, Daniotti J. Human Sialidase Neu3 is S-Acylated and Behaves Like an Integral Membrane Protein. Sci Rep. 2017;7:4167 pubmed publisher
    Membrane-bound sialidase Neu3 is involved in the catabolism of glycoconjugates, and plays crucial roles in numerous biological processes...
  24. Liu J, Liu R, Wang J, Zhang Y, Xing S, Zheng M, et al. Exploring Genomic Variants Related to Residual Feed Intake in Local and Commercial Chickens by Whole Genomic Resequencing. Genes (Basel). 2018;9: pubmed publisher
    ..Correspondingly, NOS1, PHKG1, NEU3 and PIP5K1B were differentially expressed in Beijing-You, while CDC42, CSK, PIK3R3, ..
  25. Yoshinaga A, Kajiya N, Oishi K, Kamada Y, Ikeda A, Chigwechokha P, et al. NEU3 inhibitory effect of naringin suppresses cancer cell growth by attenuation of EGFR signaling through GM3 ganglioside accumulation. Eur J Pharmacol. 2016;782:21-9 pubmed publisher
    ..Furthermore, naringin inhibited NEU3 sialidase, a GM3 degrading glycosidase...
  26. Katorcha E, Klimova N, Makarava N, Savtchenko R, Pan X, Annunziata I, et al. Loss of Cellular Sialidases Does Not Affect the Sialylation Status of the Prion Protein but Increases the Amounts of Its Proteolytic Fragment C1. PLoS ONE. 2015;10:e0143218 pubmed publisher
    ..Of the four known mammalian sialidases, the enzymes that cleave off sialic acid residues, only NEU1, NEU3 and NEU4 are expressed in the brain...
  27. Mozzi A, Forcella M, Riva A, Difrancesco C, Molinari F, Martin V, et al. NEU3 activity enhances EGFR activation without affecting EGFR expression and acts on its sialylation levels. Glycobiology. 2015;25:855-68 pubmed publisher
    ..The plasma membrane-associated sialidase NEU3 is often deregulated in colorectal cancer (CRC), and it was shown that this enzyme co-immunoprecipitates in HeLa ..
  28. D Avila F, Tringali C, Papini N, Anastasia L, Croci G, Massaccesi L, et al. Identification of lysosomal sialidase NEU1 and plasma membrane sialidase NEU3 in human erythrocytes. J Cell Biochem. 2013;114:204-11 pubmed publisher
    ..Our results demonstrate that: (a) Both sialidases NEU1 and NEU3 are present on erythrocyte plasma membrane; (b) NEU1 is kept on the plasma membrane in absence of the protective ..
  29. Ma F, Wu D, Deng L, Secrest P, Zhao J, Varki N, et al. Sialidases on mammalian sperm mediate deciduous sialylation during capacitation. J Biol Chem. 2012;287:38073-9 pubmed publisher
    ..We report the presence of two sialidases (neuraminidases Neu1 and Neu3) on mammalian sperm. These are themselves shed from sperm during capacitation...
  30. Miyata M, Kambe M, Tajima O, Moriya S, Sawaki H, Hotta H, et al. Membrane sialidase NEU3 is highly expressed in human melanoma cells promoting cell growth with minimal changes in the composition of gangliosides. Cancer Sci. 2011;102:2139-49 pubmed publisher
    NEU3 is a membrane sialidase specific for gangliosides. Its increased expression and implication in some cancers have been reported. Here, we analyzed NEU3 expression in malignant melanoma cell lines and its roles in the cancer phenotypes...
  31. Sasaki A, Hata K, Suzuki S, Sawada M, Wada T, Yamaguchi K, et al. Overexpression of plasma membrane-associated sialidase attenuates insulin signaling in transgenic mice. J Biol Chem. 2003;278:27896-902 pubmed
    ..Here we demonstrate that mice overexpressing the human ortholog (NEU3) develop diabetic phenotype by 18-22 weeks associated with hyperinsulinemia, islet hyperplasia, and increased beta-..
  32. Hata K, Tochigi T, Sato I, Kawamura S, Shiozaki K, Wada T, et al. Increased sialidase activity in serum of cancer patients: Identification of sialidase and inhibitor activities in human serum. Cancer Sci. 2015;106:383-9 pubmed publisher
    ..Among the four types identified to date, we previously demonstrated that plasma membrane-associated ganglioside sialidase (NEU3) is markedly upregulated in various human cancers, including examples in the colon and prostate...
  33. Yamamoto K, Takahashi K, Shiozaki K, Yamaguchi K, Moriya S, Hosono M, et al. Potentiation of epidermal growth factor-mediated oncogenic transformation by sialidase NEU3 leading to Src activation. PLoS ONE. 2015;10:e0120578 pubmed publisher
    We previously demonstrated that sialidase NEU3, a key glycosidase for ganglioside degradation, is up-regulated in various human cancers, leading to increased cell invasion, motility and survival of cancer cells possibly through activation ..
  34. Rodriguez Walker M, Vilcaes A, Garbarino Pico E, Daniotti J. Role of plasma-membrane-bound sialidase NEU3 in clathrin-mediated endocytosis. Biochem J. 2015;470:131-44 pubmed publisher
    ..Sialidase NEU3 is a key enzyme in the catabolism of gangliosides with its up-regulation having been observed in human cancer cells...
  35. Tringali C, Lupo B, Cirillo F, Papini N, Anastasia L, Lamorte G, et al. Silencing of membrane-associated sialidase Neu3 diminishes apoptosis resistance and triggers megakaryocytic differentiation of chronic myeloid leukemic cells K562 through the increase of ganglioside GM3. Cell Death Differ. 2009;16:164-74 pubmed publisher
    ..is also blocked because of low levels of ganglioside GM3, derived by the high expression of sialidase Neu3 active on GM3...
  36. Takahashi K, Proshin S, Yamaguchi K, Yamashita Y, Katakura R, Yamamoto K, et al. Sialidase NEU3 defines invasive potential of human glioblastoma cells by regulating calpain-mediated proteolysis of focal adhesion proteins. Biochim Biophys Acta Gen Subj. 2017;1861:2778-2788 pubmed publisher
    ..Focusing on this invasive nature, we investigated whether ganglioside-specific sialidase NEU3 might be involved, because gangliosides are major components of brain tissues, and cell surface sialic acids, as ..
  37. Shiga K, Takahashi K, Sato I, Kato K, Saijo S, Moriya S, et al. Upregulation of sialidase NEU3 in head and neck squamous cell carcinoma associated with lymph node metastasis. Cancer Sci. 2015;106:1544-53 pubmed publisher
    ..We found plasma membrane-associated sialidase (NEU3) to be significantly upregulated in tumor compared to non-tumor tissues; particularly, an increase in its mRNA ..
  38. Takahashi K, Hosono M, Sato I, Hata K, Wada T, Yamaguchi K, et al. Sialidase NEU3 contributes neoplastic potential on colon cancer cells as a key modulator of gangliosides by regulating Wnt signaling. Int J Cancer. 2015;137:1560-73 pubmed publisher
    The plasma membrane-associated sialidase NEU3 is a key enzyme for ganglioside degradation. We previously demonstrated remarkable up-regulation of NEU3 in various human cancers, with augmented malignant properties...
  39. Forcella M, Oldani M, Epistolio S, Freguia S, Monti E, Fusi P, et al. Non-small cell lung cancer (NSCLC), EGFR downstream pathway activation and TKI targeted therapies sensitivity: Effect of the plasma membrane-associated NEU3. PLoS ONE. 2017;12:e0187289 pubmed publisher
    ..g.: gefitinib). Recently, it has been shown that sialidase NEU3 directly interacts and regulates EGFR...
  40. Azuma Y, Sato H, Higai K, Matsumoto K. Enhanced expression of membrane-associated sialidase Neu3 decreases GD3 and increases GM3 on the surface of Jurkat cells during etoposide-induced apoptosis. Biol Pharm Bull. 2007;30:1680-4 pubmed
    ..N-acetylneuraminic acid and measured the mRNA expression of the plasma membrane-associated sialidase Neu3 and the lysozomal Neu1 using real-time PCR...
  41. Xie C, Yagai T, Luo Y, Liang X, Chen T, Wang Q, et al. Activation of intestinal hypoxia-inducible factor 2α during obesity contributes to hepatic steatosis. Nat Med. 2017;23:1298-1308 pubmed publisher
    ..HIF-2α regulates ceramide metabolism mainly from the salvage pathway, by positively regulating the expression of Neu3, the gene encoding neuraminidase 3...
  42. Miyagi T, Takahashi K, Shiozaki K, Yamaguchi K, Hosono M. Plasma membrane-associated sialidase confers cancer initiation, promotion and progression. Adv Exp Med Biol. 2015;842:139-45 pubmed publisher
  43. Piccoli M, Conforti E, Varrica A, Ghiroldi A, Cirillo F, Resmini G, et al. NEU3 sialidase role in activating HIF-1? in response to chronic hypoxia in cyanotic congenital heart patients. Int J Cardiol. 2017;230:6-13 pubmed publisher
    ..this line, we recently discovered and reported a novel mechanism of HIF activation, which is mediated by sialidase NEU3. Thus, aim of this study was to test whether NEU3 played any role in the cardiac cell response to chronic hypoxia ..
  44. Mandal C, Tringali C, Mondal S, Anastasia L, Chandra S, Venerando B, et al. Down regulation of membrane-bound Neu3 constitutes a new potential marker for childhood acute lymphoblastic leukemia and induces apoptosis suppression of neoplastic cells. Int J Cancer. 2010;126:337-49 pubmed publisher
    Membrane-linked sialidase Neu3 is a key enzyme for the extralysosomal catabolism of gangliosides. In this respect, it regulates pivotal cell surface events, including trans-membrane signaling, and plays an essential role in carcinogenesis...
  45. Seyrantepe V, Demir S, Timur Z, von Gerichten J, Marsching C, Erdemli E, et al. Murine Sialidase Neu3 facilitates GM2 degradation and bypass in mouse model of Tay-Sachs disease. Exp Neurol. 2018;299:26-41 pubmed publisher
    ..To determine if the sialidase NEU3 contributes to GM2 ganglioside degradation, we generated a mouse model with combined deficiencies of HEXA and NEU3...
  46. Jia F, Howlader M, Cairo C. Integrin-mediated cell migration is blocked by inhibitors of human neuraminidase. Biochim Biophys Acta. 2016;1861:1170-1179 pubmed publisher
    ..Using a scratch assay, we found that exogenously added Neu3 and Neu4 activity altered rates of cell migration...
  47. García Mateo N, Pascua Maestro R, Pérez Castellanos A, Lillo C, Sanchez D, Ganfornina M. Myelin extracellular leaflet compaction requires apolipoprotein D membrane management to optimize lysosomal-dependent recycling and glycocalyx removal. Glia. 2018;66:670-687 pubmed publisher
    ..In peripheral nervous system, Neu3 membrane sialidase and lysosomal Neu1 are coordinately expressed with ApoD in subsets of Schwann cells...
  48. Sandbhor M, Soya N, Albohy A, Zheng R, Cartmell J, Bundle D, et al. Substrate recognition of the membrane-associated sialidase NEU3 requires a hydrophobic aglycone. Biochemistry. 2011;50:6753-62 pubmed publisher
    ..The NEU3 isoform is a membrane-associated enzyme that cleaves glycolipid substrates...
  49. Rota P, La Rocca P, Piccoli M, Montefiori M, Cirillo F, Olsen L, et al. New Potent Inhibitors against Newcastle Disease Virus Haemagglutinin-Neuraminidase. ChemMedChem. 2017;: pubmed publisher
    ..Remarkably, these inhibitors resulted essentially inactive toward the human sialidase NEU3, which is present on the outer layer of the cell membrane and is highly affected by current NDV inhibitor FANA.
  50. Miyagi T, Wada T, Yamaguchi K. Roles of plasma membrane-associated sialidase NEU3 in human cancers. Biochim Biophys Acta. 2008;1780:532-7 pubmed
    ..Among human sialidases so far identified, sialidase NEU3 is a key enzyme for ganglioside degradation because of its uniqueness both in its localization in the plasma ..
  51. Yoshizumi S, Suzuki S, Hirai M, Hinokio Y, Yamada T, Yamada T, et al. Increased hepatic expression of ganglioside-specific sialidase, NEU3, improves insulin sensitivity and glucose tolerance in mice. Metabolism. 2007;56:420-9 pubmed
    ..Plasma membrane-associated sialidase, NEU3, is a key enzyme for ganglioside hydrolysis...
  52. Oehler C, Kopitz J, Cantz M. Substrate specificity and inhibitor studies of a membrane-bound ganglioside sialidase isolated from human brain tissue. Biol Chem. 2002;383:1735-42 pubmed
    ..Here we report on the specificity toward gangliosides and susceptibility to certain inhibitors of a ganglioside sialidase solubilized and purified 5100-fold from human brain...
  53. Nan X, Carubelli I, Stamatos N. Sialidase expression in activated human T lymphocytes influences production of IFN-gamma. J Leukoc Biol. 2007;81:284-96 pubmed
    ..In this study, we demonstrate that only lysosomal Neu1 and plasma membrane-associated Neu3 are detected in freshly isolated and activated human T lymphocytes...
  54. Wang J, Wu G, Miyagi T, Lu Z, Ledeen R. Sialidase occurs in both membranes of the nuclear envelope and hydrolyzes endogenous GD1a. J Neurochem. 2009;111:547-54 pubmed publisher
    ..Western blot analysis of the separated membranes revealed occurrence of Neu3 in the inner membrane and Neu1 in the outer membrane of the NE...
  55. Gadhoum S, Sackstein R. CD15 expression in human myeloid cell differentiation is regulated by sialidase activity. Nat Chem Biol. 2008;4:751-7 pubmed publisher
    ..Our results unveil a new paradigm by demonstrating a critical role for post-Golgi membrane glycosidase activity in the 'biosynthesis' of a key glycan determinant. ..
  56. Hu H, Shioda T, Moriya C, Xin X, Hasan M, Miyake K, et al. Infectivities of human and other primate lentiviruses are activated by desialylation of the virion surface. J Virol. 1996;70:7462-70 pubmed
    ..Thus, the sialylation catalyzed by host cell pathways appeared to reduce the infectivity of human and nonhuman primate lentiviruses. Our results further suggested that desialylation would help increase the titers of HIV-based vectors. ..
  57. Cirillo F, Ghiroldi A, Fania C, Piccoli M, Torretta E, Tettamanti G, et al. NEU3 Sialidase Protein Interactors in the Plasma Membrane and in the Endosomes. J Biol Chem. 2016;291:10615-24 pubmed publisher
    b>NEU3 sialidase has been shown to be a key player in many physio- and pathological processes, including cell differentiation, cellular response to hypoxic stress, and carcinogenesis...
  58. Stamatos N, Liang F, Nan X, Landry K, Cross A, Wang L, et al. Differential expression of endogenous sialidases of human monocytes during cellular differentiation into macrophages. FEBS J. 2005;272:2545-56 pubmed
    ..this study, we demonstrate that three of these sialidases, lysosomal Neu1 and Neu4 and plasma membrane-associated Neu3, are expressed in human monocytes...
  59. Yamaguchi K, Hata K, Wada T, Moriya S, Miyagi T. Epidermal growth factor-induced mobilization of a ganglioside-specific sialidase (NEU3) to membrane ruffles. Biochem Biophys Res Commun. 2006;346:484-90 pubmed
    Human ganglioside-specific sialidase, NEU3, localized at cell membranes is thought to regulate various biological processes at cell surfaces...
  60. Hart M, Saifuddin M, Uemura K, Bremer E, Hooker B, Kawasaki T, et al. High mannose glycans and sialic acid on gp120 regulate binding of mannose-binding lectin (MBL) to HIV type 1. AIDS Res Hum Retroviruses. 2002;18:1311-7 pubmed
    ..These studies provide evidence that MBL binds to HIV via high mannose carbohydrates on gp120 and shows that the interaction of MBL with virus is regulated by sialylation. ..
  61. Monti E, Bassi M, Papini N, Riboni M, Manzoni M, Venerando B, et al. Identification and expression of NEU3, a novel human sialidase associated to the plasma membrane. Biochem J. 2000;349:343-51 pubmed
    ..Here we describe the identification and expression of a human plasma-membrane-associated sialidase, NEU3, isolated starting from an expressed sequence tag (EST) clone...
  62. Paolini L, Orizio F, Busatto S, Radeghieri A, Bresciani R, Bergese P, et al. Exosomes Secreted by HeLa Cells Shuttle on Their Surface the Plasma Membrane-Associated Sialidase NEU3. Biochemistry. 2017;56:6401-6408 pubmed publisher
    ..The plasma membrane-associated sialidase NEU3 is involved in the fine-tuning of sialic acid-containing glycans directly on the cell surface and plays relevant ..
  63. Cross A, Hyun S, Miranda Ribera A, Feng C, Liu A, Nguyen C, et al. NEU1 and NEU3 sialidase activity expressed in human lung microvascular endothelia: NEU1 restrains endothelial cell migration, whereas NEU3 does not. J Biol Chem. 2012;287:15966-80 pubmed publisher
    ..b>NEU3 was detected both in the cytosol and nucleus...
  64. Ha K, Lee Y, Cho S, Kim J, Kim C. Molecular characterization of membrane type and ganglioside-specific sialidase (Neu3) expressed in E. coli. Mol Cells. 2004;17:267-73 pubmed
    Endogenous expression of human membrane type ganglioside sialidase (Neu3) was examined in various cell lines including NB-1, U87MG, SK-MEL-2, SK-N-MC, HepG2, Hep3B, Jurkat, HL-60, K562, ECV304, Hela and MCF-7...
  65. Miyagi T, Wada T, Yamaguchi K, Shiozaki K, Sato I, Kakugawa Y, et al. Human sialidase as a cancer marker. Proteomics. 2008;8:3303-11 pubmed publisher
    ..Immunohistochemistry of cancer tissues using the antibody against the plasma membrane sialidase was useful for clinical diagnosis...
  66. Jin U, Ha K, Kim K, Chang Y, Lee Y, Ko J, et al. Membrane type sialidase inhibits the megakaryocytic differentiation of human leukemia K562 cells. Biochim Biophys Acta. 2008;1780:757-63 pubmed publisher
    The membrane type sialidase (Neu3) has been suggested to participate in cell growth, migration and differentiation...
  67. Tringali C, Lupo B, Silvestri I, Papini N, Anastasia L, Tettamanti G, et al. The plasma membrane sialidase NEU3 regulates the malignancy of renal carcinoma cells by controlling ?1 integrin internalization and recycling. J Biol Chem. 2012;287:42835-45 pubmed publisher
    The human plasma membrane sialidase NEU3 is a key enzyme in the catabolism of membrane gangliosides, is crucial in the regulation of cell surface processes, and has been demonstrated to be significantly up-regulated in renal cell ..