NEK2

Summary

Gene Symbol: NEK2
Description: NIMA related kinase 2
Alias: HsPK21, NEK2A, NLK1, PPP1R111, RP67, serine/threonine-protein kinase Nek2, NIMA (never in mitosis gene a)-related kinase 2, nimA-like protein kinase 1, nimA-related protein kinase 2, protein phosphatase 1, regulatory subunit 111
Species: human
Products:     NEK2

Top Publications

  1. Schultz S, Fry A, Sutterlin C, Ried T, Nigg E. Cell cycle-dependent expression of Nek2, a novel human protein kinase related to the NIMA mitotic regulator of Aspergillus nidulans. Cell Growth Differ. 1994;5:625-35 pubmed
    ..Here, we report the isolation of complementary DNAs encoding Nek2 and Nek3, two novel human protein kinases structurally related to NIMA...
  2. Szponar A, Zubakov D, Pawlak J, Jauch A, Kovacs G. Three genetic developmental stages of papillary renal cell tumors: duplication of chromosome 1q marks fatal progression. Int J Cancer. 2009;124:2071-6 pubmed publisher
  3. Ha Kim Y, Yeol Choi J, Jeong Y, Wolgemuth D, Rhee K. Nek2 localizes to multiple sites in mitotic cells, suggesting its involvement in multiple cellular functions during the cell cycle. Biochem Biophys Res Commun. 2002;290:730-6 pubmed
    b>Nek2 is a mammalian protein kinase that is structurally homologous to NIMA, a mitotic regulator in Aspergillus nidulans...
  4. Cappello P, Blaser H, Gorrini C, Lin D, Elia A, Wakeham A, et al. Role of Nek2 on centrosome duplication and aneuploidy in breast cancer cells. Oncogene. 2014;33:2375-84 pubmed publisher
    ..One of the several genes found to be important for tumor growth in the lungs was NIMA-related kinases 2 (Nek2), a cell cycle-related protein kinase. Furthermore, Nek2 was also important for tumor growth in the mammary fat pad...
  5. Hayward D, Clarke R, Faragher A, Pillai M, Hagan I, Fry A. The centrosomal kinase Nek2 displays elevated levels of protein expression in human breast cancer. Cancer Res. 2004;64:7370-6 pubmed
    ..Finally, by ectopic expression of Nek2A in immortalized HBL100 breast epithelial cells, we show that increased Nek2 protein leads to accumulation of ..
  6. Hayward D, Fry A. Nek2 kinase in chromosome instability and cancer. Cancer Lett. 2006;237:155-66 pubmed
    ..Some of these localize to the centrosome and regulate mitotic spindle formation. One such protein is Nek2, a member of the NIMA-related serine/threonine kinase family...
  7. Lee J, Gollahon L. Nek2-targeted ASO or siRNA pretreatment enhances anticancer drug sensitivity in triple?negative breast cancer cells. Int J Oncol. 2013;42:839-47 pubmed publisher
    ..NIMA?related kinase 2 (Nek2), a serine/threonine kinase is emerging as an important oncogene because of its regulatory role in ..
  8. Quarmby L, Mahjoub M. Caught Nek-ing: cilia and centrioles. J Cell Sci. 2005;118:5161-9 pubmed
    ..Relatives of the Nek2/NIMA proteins play important roles at the G2-M transition in nuclear envelope breakdown and centromere separation...
  9. Mi J, Guo C, Brautigan D, Larner J. Protein phosphatase-1alpha regulates centrosome splitting through Nek2. Cancer Res. 2007;67:1082-9 pubmed
    ..We recently showed that protein phosphatase 1 (PP1) is activated by ATM. Because Nek2 is activated by autophosphorylation, and because its dephosphorylation is catalyzed by PP1, we asked if the ..

More Information

Publications79

  1. Wang S, Li W, Liu N, Zhang F, Liu H, Liu F, et al. Nek2A contributes to tumorigenic growth and possibly functions as potential therapeutic target for human breast cancer. J Cell Biochem. 2012;113:1904-14 pubmed publisher
    b>Nek2A (NIMA-related kinases 2A) has been known as an important centrosome regulatory factor. The aim of this study was to investigate the expression of Nek2A and the role it played in different stages of breast cancer...
  2. Wang S, Li W, Lv S, Wang Y, Liu Z, Zhang J, et al. Abnormal expression of Nek2 and ?-catenin in breast carcinoma: clinicopathological correlations. Histopathology. 2011;59:631-42 pubmed publisher
    NIMA-related kinase 2 (Nek2) and ?-catenin are important centrosome regulatory factors...
  3. Helps N, Luo X, Barker H, Cohen P. NIMA-related kinase 2 (Nek2), a cell-cycle-regulated protein kinase localized to centrosomes, is complexed to protein phosphatase 1. Biochem J. 2000;349:509-18 pubmed
    The cell cycle-regulated protein serine/threonine NIMA-related kinase 2 (Nek2), which shows a predominant localization at centrosomes, is identified as a protein which interacts with protein phosphatase 1 (PP1) using the yeast two-hybrid ..
  4. Fry A, Arnaud L, Nigg E. Activity of the human centrosomal kinase, Nek2, depends on an unusual leucine zipper dimerization motif. J Biol Chem. 1999;274:16304-10 pubmed
    b>Nek2 is a human cell cycle-regulated kinase that is structurally related to the mitotic regulator, NIMA, of Aspergillus nidulans...
  5. Fry A, Mayor T, Meraldi P, Stierhof Y, Tanaka K, Nigg E. C-Nap1, a novel centrosomal coiled-coil protein and candidate substrate of the cell cycle-regulated protein kinase Nek2. J Cell Biol. 1998;141:1563-74 pubmed
    b>Nek2 (for NIMA-related kinase 2) is a mammalian cell cycle-regulated kinase structurally related to the mitotic regulator NIMA of Aspergillus nidulans...
  6. Wei R, Ngo B, Wu G, Lee W. Phosphorylation of the Ndc80 complex protein, HEC1, by Nek2 kinase modulates chromosome alignment and signaling of the spindle assembly checkpoint. Mol Biol Cell. 2011;22:3584-94 pubmed publisher
    ..A small portion of S165E cells eventually bypassed the SAC but showed severe segregation errors. Nek2 is the primary kinase responsible for kinetochore pS165, while PP1 phosphatase may dephosphorylate pS165 during SAC ..
  7. Zeng X, Shaikh F, Harrison M, Adon A, Trimboli A, Carroll K, et al. The Ras oncogene signals centrosome amplification in mammary epithelial cells through cyclin D1/Cdk4 and Nek2. Oncogene. 2010;29:5103-12 pubmed publisher
    ..in vivo and in vitro, was associated with increased expression of the centrosome-regulatory proteins, cyclin D1 and Nek2. Abolishing the expression of cyclin D1, Cdk4 or Nek2 in MCF10A human mammary epithelial cells expressing H-Ras(..
  8. Rellos P, Ivins F, Baxter J, Pike A, Nott T, Parkinson D, et al. Structure and regulation of the human Nek2 centrosomal kinase. J Biol Chem. 2007;282:6833-42 pubmed
    The dimeric Ser/Thr kinase Nek2 regulates centrosome cohesion and separation through phosphorylation of structural components of the centrosome, and aberrant regulation of Nek2 activity can lead to aneuploid defects characteristic of ..
  9. Hayes M, Kimata Y, Wattam S, Lindon C, Mao G, Yamano H, et al. Early mitotic degradation of Nek2A depends on Cdc20-independent interaction with the APC/C. Nat Cell Biol. 2006;8:607-14 pubmed
    ..Here, we show that another APC/C substrate, NIMA-related kinase 2A (Nek2A), is also destroyed in pro-metaphase in a checkpoint-independent manner and that this depends on an exposed ..
  10. Du J, Cai X, Yao J, Ding X, Wu Q, Pei S, et al. The mitotic checkpoint kinase NEK2A regulates kinetochore microtubule attachment stability. Oncogene. 2008;27:4107-14 pubmed publisher
    ..Our studies show that NEK2A is a kinetochore-associated protein kinase essential for faithful chromosome segregation...
  11. Tsunoda N, Kokuryo T, Oda K, Senga T, Yokoyama Y, Nagino M, et al. Nek2 as a novel molecular target for the treatment of breast carcinoma. Cancer Sci. 2009;100:111-6 pubmed publisher
    We investigated the role of Nek2, a member of the serine-threonine kinase family, in the tumorigenic growth of breast carcinoma...
  12. Liu X, Gao Y, Lu Y, Zhang J, Li L, Yin F. Upregulation of NEK2 is associated with drug resistance in ovarian cancer. Oncol Rep. 2014;31:745-54 pubmed publisher
    b>NEK2 [NIMA (never in mitosis gene A)-related expressed kinase 2] is associated with various biological behaviors in the development of cancer, while research concerning its association with drug resistance is limited...
  13. Noguchi K, Fukazawa H, Murakami Y, Uehara Y. Nucleolar Nek11 is a novel target of Nek2A in G1/S-arrested cells. J Biol Chem. 2004;279:32716-27 pubmed
    ..We provide herein several lines of evidence for a novel interaction between Nek11 and Nek2A. Both Nek11 and Nek2A, but not Nek2B, were detected at nucleoli, and the Nek2A-specific C-terminal end (amino ..
  14. Lou Y, Yao J, Zereshki A, Dou Z, Ahmed K, Wang H, et al. NEK2A interacts with MAD1 and possibly functions as a novel integrator of the spindle checkpoint signaling. J Biol Chem. 2004;279:20049-57 pubmed
    ..Here we report that spindle checkpoint protein MAD1 interacts with NEK2A, a human orthologue of the Aspergillus nidulans NIMA kinase...
  15. Faragher A, Fry A. Nek2A kinase stimulates centrosome disjunction and is required for formation of bipolar mitotic spindles. Mol Biol Cell. 2003;14:2876-89 pubmed
    b>Nek2A is a cell cycle-regulated kinase of the never in mitosis A (NIMA) family that is highly enriched at the centrosome...
  16. Zhong X, Guan X, Dong Q, Yang S, Liu W, Zhang L. Examining Nek2 as a better proliferation marker in non-small cell lung cancer prognosis. Tumour Biol. 2014;35:7155-62 pubmed publisher
    ..The expressions of Nek2, MCM7, and Ki-67 were evaluated in 270 NSCLC tissues using immunohistochemical and immunofluorescence techniques...
  17. Eto M, Elliott E, Prickett T, Brautigan D. Inhibitor-2 regulates protein phosphatase-1 complexed with NimA-related kinase to induce centrosome separation. J Biol Chem. 2002;277:44013-20 pubmed
    ..The mammalian NimA-related kinase Nek2 forms a complex with the catalytic subunit of protein phosphatase-1 (PP1C)...
  18. Hames R, Wattam S, Yamano H, Bacchieri R, Fry A. APC/C-mediated destruction of the centrosomal kinase Nek2A occurs in early mitosis and depends upon a cyclin A-type D-box. EMBO J. 2001;20:7117-27 pubmed
    ..Here we show that Nek2A, but not Nek2B, is destroyed upon entry into mitosis coincident with cyclin A destruction and in the presence of ..
  19. Hames R, Fry A. Alternative splice variants of the human centrosome kinase Nek2 exhibit distinct patterns of expression in mitosis. Biochem J. 2002;361:77-85 pubmed
    ..These isoforms, designated Nek2A and Nek2B, are detected in primary blood lymphocytes as well as adult transformed cells...
  20. Fry A, Meraldi P, Nigg E. A centrosomal function for the human Nek2 protein kinase, a member of the NIMA family of cell cycle regulators. EMBO J. 1998;17:470-81 pubmed
    b>Nek2, a mammalian protein kinase of unknown function, is closely related to the mitotic regulator NIMA of Aspergillus nidulans...
  21. Chen Y, Sharp Z, Lee W. HEC binds to the seventh regulatory subunit of the 26 S proteasome and modulates the proteolysis of mitotic cyclins. J Biol Chem. 1997;272:24081-7 pubmed
    ..Complementary DNAs encoding several proteins including MSS1, p45, Nek2, and Smc1/Smc2, known to be important for G2/M progression, were identified...
  22. Stricker T, Henriksen K, Tonsgard J, Montag A, Krausz T, Pytel P. Expression profiling of 519 kinase genes in matched malignant peripheral nerve sheath tumor/plexiform neurofibroma samples is discriminatory and identifies mitotic regulators BUB1B, PBK and NEK2 as overexpressed with transformation. Mod Pathol. 2013;26:930-43 pubmed publisher
    ..Immunohistochemical studies for the mitotic regulators BUB1B, PBK and NEK2 confirm higher expression levels at the protein level...
  23. Sedgwick G, Hayward D, Di Fiore B, Pardo M, Yu L, Pines J, et al. Mechanisms controlling the temporal degradation of Nek2A and Kif18A by the APC/C-Cdc20 complex. EMBO J. 2013;32:303-14 pubmed publisher
    ..b>Nek2A degradation during prometaphase depends on direct binding of Nek2A to the APC/C via a C-terminal MR dipeptide but ..
  24. Mardin B, Agircan F, Lange C, Schiebel E. Plk1 controls the Nek2A-PP1? antagonism in centrosome disjunction. Curr Biol. 2011;21:1145-51 pubmed publisher
    ..At the onset of mitosis, these linker proteins are phosphorylated and displaced from centrosomes by the Nek2A kinase, which is regulated by two Hippo pathway components, Mst2 kinase and the scaffold protein hSav1...
  25. Mbom B, Siemers K, Ostrowski M, Nelson W, Barth A. Nek2 phosphorylates and stabilizes ?-catenin at mitotic centrosomes downstream of Plk1. Mol Biol Cell. 2014;25:977-91 pubmed publisher
    ..NIMA-related protein kinase 2 (Nek2), which regulates centrosome disjunction/splitting, binds to and phosphorylates ?-catenin...
  26. Mardin B, Lange C, Baxter J, Hardy T, Scholz S, Fry A, et al. Components of the Hippo pathway cooperate with Nek2 kinase to regulate centrosome disjunction. Nat Cell Biol. 2010;12:1166-76 pubmed publisher
    ..The NIMA (never in mitosis A)-related kinase Nek2A is implicated in disconnecting the centrosomes through disjoining the linker proteins C-Nap1 and rootletin...
  27. Wu W, Baxter J, Wattam S, Hayward D, Fardilha M, Knebel A, et al. Alternative splicing controls nuclear translocation of the cell cycle-regulated Nek2 kinase. J Biol Chem. 2007;282:26431-40 pubmed
    ..Two major splice variants have been described in vertebrates, Nek2A and Nek2B, that differ in their non-catalytic C termini...
  28. Chen Y, Riley D, Zheng L, Chen P, Lee W. Phosphorylation of the mitotic regulator protein Hec1 by Nek2 kinase is essential for faithful chromosome segregation. J Biol Chem. 2002;277:49408-16 pubmed
    ..we show that human Hec1 is a serine phosphoprotein and that it binds specifically to the mitotic regulatory kinase Nek2 during G(2)/M. Nek2 phosphorylates Hec1 on serine residue 165, both in vitro and in vivo...
  29. Bahmanyar S, Kaplan D, DeLuca J, Giddings T, O TOOLE E, Winey M, et al. beta-Catenin is a Nek2 substrate involved in centrosome separation. Genes Dev. 2008;22:91-105 pubmed
    ..Centrosomes are held together by a dynamic linker regulated by Nek2 kinase and its substrates C-Nap1 (centrosomal Nek2-associated protein 1) and Rootletin...
  30. Fletcher L, Cerniglia G, Yen T, Muschel R. Live cell imaging reveals distinct roles in cell cycle regulation for Nek2A and Nek2B. Biochim Biophys Acta. 2005;1744:89-92 pubmed
    Two splice variants of Nek2 kinase, a member of the NIMA-related family, have been identified as Nek2A and Nek2B. Nek2A regulates centrosome disjunction, spindle formation checkpoint signaling, and faithful chromosome segregation...
  31. Wu S, Lin S, Lee K, Chuang H, Feng P, Cheng W, et al. Hepatoma cell functions modulated by NEK2 are associated with liver cancer progression. Int J Cancer. 2017;140:1581-1596 pubmed publisher
    b>NEK2 (NIMA-related expressed kinase 2) is a serine/threonine centrosomal kinase that acts as a critical regulator of centrosome structure and function. Aberrant NEK2 activities lead to failure in regulating centrosome duplication...
  32. Arai E, Gotoh M, Tian Y, Sakamoto H, Ono M, Matsuda A, et al. Alterations of the spindle checkpoint pathway in clinicopathologically aggressive CpG island methylator phenotype clear cell renal cell carcinomas. Int J Cancer. 2015;137:2589-606 pubmed publisher
    ..e., AURKA, AURKB, BIRC5, BUB1, CDC20, NEK2 and SPC25, were significantly higher in CIMP-positive than in CIMP-negative RCCs...
  33. Chen C, Tian F, Lu L, Wang Y, Xiao Z, Yu C, et al. Characterization of Cep85 - a new antagonist of Nek2A that is involved in the regulation of centrosome disjunction. J Cell Sci. 2015;128:3290-303 pubmed publisher
    ..In this study, we report that Cep85, an uncharacterized centrosomal protein, acts as a binding partner of Nek2A. It colocalizes with isoform A of Nek2 (Nek2A) at centrosomes and forms a granule meshwork enveloping the proximal ..
  34. Fardilha M, Wu W, Sá R, Fidalgo S, Sousa C, Mota C, et al. Alternatively spliced protein variants as potential therapeutic targets for male infertility and contraception. Ann N Y Acad Sci. 2004;1030:468-78 pubmed
    ..Among the various proteins detected, the most abundant interactors with PP1gamma2 were Nek2A and R15B...
  35. Wang J, Cheng P, Pavlyukov M, Yu H, Zhang Z, Kim S, et al. Targeting NEK2 attenuates glioblastoma growth and radioresistance by destabilizing histone methyltransferase EZH2. J Clin Invest. 2017;127:3075-3089 pubmed publisher
    ..In this study, we identified NIMA-related kinase 2 (NEK2) as a functional binding protein of enhancer of zeste homolog 2 (EZH2) that plays a critical role in the ..
  36. Croasdale R, Ivins F, Muskett F, Daviter T, Scott D, Hardy T, et al. An undecided coiled coil: the leucine zipper of Nek2 kinase exhibits atypical conformational exchange dynamics. J Biol Chem. 2011;286:27537-47 pubmed publisher
    ..The leucine zipper of the cell cycle-regulated Nek2 kinase is important for its dimerization and activation...
  37. Li G, Zhong Y, Shen Q, Zhou Y, Deng X, Li C, et al. NEK2 serves as a prognostic biomarker for hepatocellular carcinoma. Int J Oncol. 2017;50:405-413 pubmed publisher
    Never in mitosis gene A (NIMA)-related kinase 2 (NEK2) is a microtubule-associated protein that regulates spindle assembly in human cells and is overexpressed in various malignancies...
  38. Jeong Y, Lee J, Kim K, Yoo J, Rhee K. Characterization of NIP2/centrobin, a novel substrate of Nek2, and its potential role in microtubule stabilization. J Cell Sci. 2007;120:2106-16 pubmed
    b>Nek2 is a mitotic kinase whose activity varies during the cell cycle...
  39. Gupta P, Bastikar V, Kučiauskas D, Kothari S, Cicenas J, Valius M. Molecular modeling and structure-based drug discovery approach reveals protein kinases as off-targets for novel anticancer drug RH1. Med Oncol. 2017;34:176 pubmed publisher
    ..as alignment of their pharmacophoric maps let us predict and ranked protein kinases such as KIT, CDK2, CDK6, MAPK1, NEK2 and others as the most prominent off-targets of RH1...
  40. Wu G, Qiu X, Zhou L, Zhu J, Chamberlin R, Lau J, et al. Small molecule targeting the Hec1/Nek2 mitotic pathway suppresses tumor cell growth in culture and in animal. Cancer Res. 2008;68:8393-9 pubmed publisher
    ..a small molecule, N-(4-[2,4-dimethyl-phenyl]-thiazol-2-yl)-benzamide (INH1), which specifically disrupts the Hec1/Nek2 interaction via direct Hec1 binding...
  41. Ning Z, Wang A, Liang J, Liu J, Zhou T, Yan Q, et al. Abnormal expression of Nek2 in pancreatic ductal adenocarcinoma: a novel marker for prognosis. Int J Clin Exp Pathol. 2014;7:2462-9 pubmed
    b>Nek2 is a serine/threonine kinase that has a critical role in mitosis during the cell division process...
  42. Krämer A, Mailand N, Lukas C, Syljuåsen R, Wilkinson C, Nigg E, et al. Centrosome-associated Chk1 prevents premature activation of cyclin-B-Cdk1 kinase. Nat Cell Biol. 2004;6:884-91 pubmed
  43. Gu Z, Xia J, Xu H, Frech I, Tricot G, Zhan F. NEK2 Promotes Aerobic Glycolysis in Multiple Myeloma Through Regulating Splicing of Pyruvate Kinase. J Hematol Oncol. 2017;10:17 pubmed publisher
    ..Co-IP) were used to study the interaction between NIMA (never in mitosis gene A)-related kinase 2 (NEK2) and heterogeneous nuclear ribonucleoproteins (hnRNP) A1/2...
  44. Cui C, Zang T, Cao Y, Qin X, Zhang X. CDC25B is involved in the centrosomal microtubule nucleation in two-cell stage mouse embryos. Dev Growth Differ. 2016;58:714-726 pubmed publisher
    ..addition, embryos injected with anti-CDC25B antibody showed the marked absence of microtubule repolymerization and Nek2 foci after nocodazole washout. CDC25B overexpression caused microtubule-organizing center (MTOC) overduplication...
  45. Hitti E, Bakheet T, Al Souhibani N, Moghrabi W, Al Yahya S, Al Ghamdi M, et al. Systematic Analysis of AU-Rich Element Expression in Cancer Reveals Common Functional Clusters Regulated by Key RNA-Binding Proteins. Cancer Res. 2016;76:4068-80 pubmed publisher
    ..and functional enrichment clustering identified a cluster of 11 overexpressed ARE-mRNAs (CDC6, KIF11, PRC1, NEK2, NCAPG, CENPA, NUF2, KIF18A, CENPE, PBK, TOP2A) that negatively correlated with TTP/HuR mRNA ratios and was ..
  46. Schultz S, Nigg E. Identification of 21 novel human protein kinases, including 3 members of a family related to the cell cycle regulator nimA of Aspergillus nidulans. Cell Growth Differ. 1993;4:821-30 pubmed
  47. Lee M, Moreno C, Saavedra H. E2F activators signal and maintain centrosome amplification in breast cancer cells. Mol Cell Biol. 2014;34:2581-99 pubmed
    ..Our results revealed that E2Fs affect the expression of proteins, including Nek2 and Plk4, known to influence the cell/centrosome cycles and mitosis...
  48. Makiyama T, Higashi S, Sakane H, Nogami S, Shirataki H. ?-Taxilin temporally regulates centrosome disjunction in a Nek2A-dependent manner. Exp Cell Res. 2018;362:412-423 pubmed publisher
    Never in mitosis A-related kinase 2A (Nek2A), a centrosomal serine/threonine kinase, is involved in mitotic progression by regulating the centrosome cycle...
  49. Kim S, Hwang S, Lee M, Kwak H, Son K, Yang J, et al. Fanconi anemia complementation group A (FANCA) localizes to centrosomes and functions in the maintenance of centrosome integrity. Int J Biochem Cell Biol. 2013;45:1953-61 pubmed publisher
    ..The candidate proteins included never-in-mitosis-gene A (NIMA)-related kinase 2 (Nek2), which functions in the maintenance of centrosome integrity...
  50. Zhang M, Xu X, Zhang P, Han N, Deng J, Yu T, et al. Effect of silencing NEK2 on biological behaviors of HepG2 in human hepatoma cells and MAPK signal pathway. Tumour Biol. 2016;37:2023-35 pubmed publisher
    To investigate the expression level of NEK2 in 40 tissue specimens of primary liver cancer and to search for clues whether the effect of NEK2 depletion plays a role on biological behaviors of HepG2 cells and the relevant molecular ..
  51. Zhao D, Han W, Liu X, Cui D, Chen Y. MicroRNA-128 promotes apoptosis in lung cancer by directly targeting NIMA-related kinase 2. Thorac Cancer. 2017;8:304-311 pubmed publisher
    ..NIMA-related kinase 2 (NEK2) is aberrantly expressed in lung cancer...
  52. Dechtawewat T, Paemanee A, Roytrakul S, Songprakhon P, Limjindaporn T, Yenchitsomanus P, et al. Mass spectrometric analysis of host cell proteins interacting with dengue virus nonstructural protein 1 in dengue virus-infected HepG2 cells. Biochim Biophys Acta. 2016;1864:1270-1280 pubmed publisher
    ..and colocalization assays confirmed the interactions of DENV NS1 and human NIMA-related kinase 2 (NEK2), thousand and one amino acid protein kinase 1 (TAO1), and component of oligomeric Golgi complex 1 (COG1) proteins ..
  53. Sedgwick G, Townsend K, Martin A, Shimwell N, Grand R, Stewart G, et al. Transcriptional intermediary factor 1? binds to the anaphase-promoting complex/cyclosome and promotes mitosis. Oncogene. 2013;32:4622-33 pubmed publisher
    ..Together these data define novel functions for TIF1? during mitosis and suggest that a reduction in APC/C ubiquitin ligase activity promotes SAC activation. ..
  54. Zhang W, Fletcher L, Muschel R. The role of Polo-like kinase 1 in the inhibition of centrosome separation after ionizing radiation. J Biol Chem. 2005;280:42994-9 pubmed
    ..This inhibition is dependent on ATM, but not on Chk2 or Chk1. Nek2 is a key regulator of centrosome separation and is a target of Plk1 in blocking centrosome separation...
  55. Fu L, Liu S, Wang H, Ma Y, Li L, He X, et al. Low expression of NEK2 is associated with hepatocellular carcinoma progression and poor prognosis. Cancer Biomark. 2017;20:101-106 pubmed publisher
    NIMA-related kinase 2 (NEK2), a serine/threonine kinase, is located in the centrosome and is a member of cell cycle regulation related protein kinase (CCRK) family...
  56. Nishiguchi K, Tearle R, Liu Y, Oh E, Miyake N, Benaglio P, et al. Whole genome sequencing in patients with retinitis pigmentosa reveals pathogenic DNA structural changes and NEK2 as a new disease gene. Proc Natl Acad Sci U S A. 2013;110:16139-44 pubmed publisher
    ..L206fs), absent in more than 2,500 chromosomes from ethnically matched controls, in the ciliary gene NEK2, encoding a serine/threonine-protein kinase...
  57. Kudoh A, Takahama S, Sawasaki T, Ode H, Yokoyama M, Okayama A, et al. The phosphorylation of HIV-1 Gag by atypical protein kinase C facilitates viral infectivity by promoting Vpr incorporation into virions. Retrovirology. 2014;11:9 pubmed publisher
    ..Furthermore, aPKC inhibition efficiently suppresses HIV-1 infectivity in macrophages. aPKC may therefore be an intriguing therapeutic target for HIV-1 infection. ..
  58. Liu H, Liu B, Hou X, Pang B, Guo P, Jiang W, et al. Overexpression of NIMA-related kinase 2 is associated with poor prognoses in malignant glioma. J Neurooncol. 2017;132:409-417 pubmed publisher
    Eleated expression of NIMA-related kinase 2 (NEK2) was frequently observed in a variety of malignant cancers, and it appears to be involved in the initiation, maintenance, progression, metastasis of cancer and is positively associated ..
  59. Lin S, Zhou S, Jiang S, Liu X, Wang Y, Zheng X, et al. NEK2 regulates stem-like properties and predicts poor prognosis in hepatocellular carcinoma. Oncol Rep. 2016;36:853-62 pubmed publisher
    b>NEK2 has been estimated to play an important role in cancer progression. However, its relevance in hepatocellular carcinoma (HCC) has not yet been explored. Immunohistochemistry revealed NEK2 expression was upregulated in HCC...
  60. Liu Y, Xue W, Zhu L, Ye D, Zhu X, Wang H, et al. Nanog suppresses the expression of vasa by directly regulating nlk1 in the early zebrafish embryo. Biochimie. 2017;142:93-101 pubmed publisher
    ..the present study, we demonstrate that zebrafish Nanog (zNanog) directly binds to the promoter region of zebrafish nlk1 (znlk1) by ChIP-Seq analysis and that it up-regulates the expression of znlk1 in fibroblast-like embryonic cells of ..
  61. Fu G, Ding X, Yuan K, Aikhionbare F, Yao J, Cai X, et al. Phosphorylation of human Sgo1 by NEK2A is essential for chromosome congression in mitosis. Cell Res. 2007;17:608-18 pubmed
    ..Our recent study shows that NEK2A interacts with MAD1 at the kinetochore and possibly functions as a novel integrator of spindle checkpoint ..
  62. Matsuo K, Nishimura T, Hayakawa A, Ono Y, Takahashi M. Involvement of a centrosomal protein kendrin in the maintenance of centrosome cohesion by modulating Nek2A kinase activity. Biochem Biophys Res Commun. 2010;398:217-23 pubmed publisher
    ..At late G2 phase centrosome cohesion is disassembled by Nek2A kinase-mediated phosphorylation and, as a consequence, centrosomes are split and constitute spindle poles in ..
  63. Kohler M, Johnson B, Palen K, Chen Q, Khan J, Orentas R. Tumor antigen analysis in neuroblastoma by serological interrogation of bioinformatic data. Cancer Sci. 2010;101:2316-24 pubmed publisher
    ..Through this procedure, the never in mitosis A (NIMA)-related kinase NEK2 was identified as a tumor-associated antigen...
  64. Wang H, Xie Y, Han J, Ruan Y, Song A, Zheng L, et al. Genetic polymorphisms in centrobin and Nek2 are associated with breast cancer susceptibility in a Chinese Han population. Breast Cancer Res Treat. 2012;136:241-51 pubmed publisher
    ..The Centrobin and Nek2 proteins interact with each other and both are involved in centrosome duplication and chromosome segregation...
  65. Lee S, Kim J, Nam H, Gao B, Yin P, Qin B, et al. Parkin Regulates Mitosis and Genomic Stability through Cdc20/Cdh1. Mol Cell. 2015;60:21-34 pubmed publisher
    ..Parkin deficiency results in overexpression of its substrates, mitotic defects, genomic instability, and tumorigenesis. These results suggest that the Parkin-Cdc20/Cdh1 complex is an important regulator of mitosis. ..
  66. Kim S, Lee K, Choi J, Ringstad N, Dynlacht B. Nek2 activation of Kif24 ensures cilium disassembly during the cell cycle. Nat Commun. 2015;6:8087 pubmed publisher
    ..We show that co-localization of the S/G2 phase kinase, Nek2 and Kif24 triggers Kif24 phosphorylation, inhibiting cilia formation...
  67. Meng L, Carpenter K, Mollard A, Vankayalapati H, Warner S, Sharma S, et al. Inhibition of Nek2 by small molecules affects proteasome activity. Biomed Res Int. 2014;2014:273180 pubmed publisher
    b>Nek2 is a serine/threonine kinase localized to the centrosome. It promotes cell cycle progression from G2 to M by inducing centrosome separation...
  68. Takahashi Y, Iwaya T, Sawada G, Kurashige J, Matsumura T, Uchi R, et al. Up-regulation of NEK2 by microRNA-128 methylation is associated with poor prognosis in colorectal cancer. Ann Surg Oncol. 2014;21:205-12 pubmed publisher
    NIMA-related kinase 2 (NEK2), an enzyme involved in the development and progression of cancer, is abnormally expressed in a wide variety of human cancers, including colorectal cancer (CRC), and is known to have roles in cell division and ..
  69. Ferreira Fernandes E, Esteves S, Korrodi Gregório L, Lüers G, Afreixo V, Fardilha M, et al. Synphilin-1A is a phosphoprotein phosphatase 1-interacting protein and affects PPP1 sorting to subcellular compartments. J Mol Neurosci. 2015;55:385-95 pubmed publisher
    ..Contrarily, in physiological conditions, Sph1 isoforms are pointed as putative participants in vesicle dynamics with implications in neurotransmission and spermiogenesis. ..
  70. Marina M, Saavedra H. Nek2 and Plk4: prognostic markers, drivers of breast tumorigenesis and drug resistance. Front Biosci (Landmark Ed). 2014;19:352-65 pubmed
    The Nek2 and Plk4 kinases serve as crucial regulators of mitotic processes such as the centrosome duplication cycle and spindle assembly...