Gene Symbol: NEDD9
Description: neural precursor cell expressed, developmentally down-regulated 9
Alias: CAS-L, CAS2, CASL, CASS2, HEF1, enhancer of filamentation 1, Cas scaffolding protein family member 2, Crk-associated substrate related protein Cas-L, Enhancer of filamentation 1 p55, cas-like docking, neural precursor cell expressed developmentally down-regulated protein 9, p130Cas-related protein, renal carcinoma antigen NY-REN-12
Species: human
Products:     NEDD9

Top Publications

  1. Manie S, Beck A, Astier A, Law S, Canty T, Hirai H, et al. Involvement of p130(Cas) and p105(HEF1), a novel Cas-like docking protein, in a cytoskeleton-dependent signaling pathway initiated by ligation of integrin or antigen receptor on human B cells. J Biol Chem. 1997;272:4230-6 pubmed
    The Crk-associated substrate p130(Cas) (Cas) and the recently described human enhancer of filamentation 1 (HEF1) are two proteins with similar structure (64% amino acid homology), which are thought to act as "docking" molecules ..
  2. Singh M, Cowell L, Seo S, O Neill G, Golemis E. Molecular basis for HEF1/NEDD9/Cas-L action as a multifunctional co-ordinator of invasion, apoptosis and cell cycle. Cell Biochem Biophys. 2007;48:54-72 pubmed
    Upregulation of the scaffolding protein HEF1, also known as NEDD9 and Cas-L, has recently been identified as a pro-metastatic stimulus in a number of different solid tumors, and has also been strongly associated with pathogenesis of BCR-..
  3. Dadke D, Jarnik M, Pugacheva E, Singh M, Golemis E. Deregulation of HEF1 impairs M-phase progression by disrupting the RhoA activation cycle. Mol Biol Cell. 2006;17:1204-17 pubmed
    The focal adhesion-associated signaling protein HEF1 undergoes a striking relocalization to the spindle at mitosis, but a function for HEF1 in mitotic signaling has not been demonstrated...
  4. Thao L, Vu H, Yasuda K, Taniguchi S, Yagasaki F, Taguchi T, et al. Cas-L was overexpressed in imatinib-resistant gastrointestinal stromal tumor cells. Cancer Biol Ther. 2009;8:683-8 pubmed
    ..We report for the first time that the mechanism of imatinib-resistant GISTs, at least in one cell line, involves KIT/Cas-L/SRC signaling. Cas-L depletion sensitized the resistant GIST-T1 IR cells to imatinib. ..
  5. Nourry C, Maksumova L, Pang M, Liu X, Wang T. Direct interaction between Smad3, APC10, CDH1 and HEF1 in proteasomal degradation of HEF1. BMC Cell Biol. 2004;5:20 pubmed
    ..and also the novel ability of regulating the proteasomal degradation of two Smad3 interacting proteins, SnoN and HEF1. It has been shown that Smad3 recruits two types of Ub E3 ligases, Smurf2 and the Anaphase Promoting Complex (APC), ..
  6. Miyake Nishijima R, Iwata S, Saijo S, Kobayashi H, Kobayashi S, Souta Kuribara A, et al. Role of Crk-associated substrate lymphocyte type in the pathophysiology of rheumatoid arthritis in tax transgenic mice and in humans. Arthritis Rheum. 2003;48:1890-900 pubmed
    ..Furthermore, in human RA, Cas-L-positive lymphocytes were shown to infiltrate to the inflammatory lesions. These results strongly suggest that Cas-L plays an important role in the pathophysiology of RA. ..
  7. Zheng M, McKeown Longo P. Regulation of HEF1 expression and phosphorylation by TGF-beta 1 and cell adhesion. J Biol Chem. 2002;277:39599-608 pubmed
    ..Human enhancer of filamentation-1 (HEF1), a multifunctional docking protein, is involved in integrin-based signaling, which affects cell motility, growth, ..
  8. Bui L, Tomkiewicz C, Chevallier A, Pierre S, Bats A, Mota S, et al. Nedd9/Hef1/Cas-L mediates the effects of environmental pollutants on cell migration and plasticity. Oncogene. 2009;28:3642-51 pubmed publisher
    ..Here, we show that dioxin-mediated activation of Ahr induces Nedd9/Hef1/Cas-L, a member of the Cas protein family recently identified as a metastasis marker...
  9. Xia D, Holla V, Wang D, Menter D, DuBois R. HEF1 is a crucial mediator of the proliferative effects of prostaglandin E(2) on colon cancer cells. Cancer Res. 2010;70:824-31 pubmed publisher
    ..we define a critical mechanism in this process by showing that the prometastatic adapter protein human enhancer of filamentation 1 (HEF1; NEDD9) links PGE(2) to the cell cycle machinery in colorectal cancer cells...

More Information


  1. Sanz Moreno V, Gadea G, Ahn J, Paterson H, Marra P, Pinner S, et al. Rac activation and inactivation control plasticity of tumor cell movement. Cell. 2008;135:510-23 pubmed publisher
    ..mesenchymal-type movement in melanoma cells is driven by activation of the GTPase Rac through a complex containing NEDD9, a recently identified melanoma metastasis gene, and DOCK3, a Rac guanine nucleotide exchange factor...
  2. Pugacheva E, Jablonski S, Hartman T, Henske E, Golemis E. HEF1-dependent Aurora A activation induces disassembly of the primary cilium. Cell. 2007;129:1351-63 pubmed
    ..Here, we show that interactions between the prometastatic scaffolding protein HEF1/Cas-L/NEDD9 and the oncogenic Aurora A (AurA) kinase at the basal body of cilia causes phosphorylation and activation of HDAC6,..
  3. Pugacheva E, Golemis E. The focal adhesion scaffolding protein HEF1 regulates activation of the Aurora-A and Nek2 kinases at the centrosome. Nat Cell Biol. 2005;7:937-46 pubmed
    Although HEF1 has a well-defined role in integrin-dependent attachment signalling at focal adhesions, it relocalizes to the spindle asters at mitosis...
  4. Feng L, Guedes S, Wang T. Atrophin-1-interacting protein 4/human Itch is a ubiquitin E3 ligase for human enhancer of filamentation 1 in transforming growth factor-beta signaling pathways. J Biol Chem. 2004;279:29681-90 pubmed
    ..Human enhancer of filamentation 1 (HEF1) has been implicated in signaling pathways such as those mediated by integrin, T cell receptor, ..
  5. Liu X, Elia A, Law S, Golemis E, Farley J, Wang T. A novel ability of Smad3 to regulate proteasomal degradation of a Cas family member HEF1. EMBO J. 2000;19:6759-69 pubmed
    ..Here we report a novel property of Smad3 in regulating the proteasomal degradation of the human enhancer of filamentation 1 (HEF1), which is a member of the Cas family of cytoplasmic docking proteins...
  6. Li Y, Bavarva J, Wang Z, Guo J, Qian C, Thibodeau S, et al. HEF1, a novel target of Wnt signaling, promotes colonic cell migration and cancer progression. Oncogene. 2011;30:2633-43 pubmed publisher
    ..Altered expression of human enhancer of filamentation 1 (HEF1; also known as NEDD9 or Cas-L) has been implicated in progression of melanoma, breast, and CRC...
  7. Law S, Estojak J, Wang B, Mysliwiec T, Kruh G, Golemis E. Human enhancer of filamentation 1, a novel p130cas-like docking protein, associates with focal adhesion kinase and induces pseudohyphal growth in Saccharomyces cerevisiae. Mol Cell Biol. 1996;16:3327-37 pubmed
    ..In this report, we describe the isolation and characterization of human enhancer of filamentation 1 (HEF1), an SH3-domain-containing protein that is similar in structure to pl30cas, a recently identified ..
  8. Tikhmyanova N, Golemis E. NEDD9 and BCAR1 negatively regulate E-cadherin membrane localization, and promote E-cadherin degradation. PLoS ONE. 2011;6:e22102 pubmed publisher
    The Cas scaffolding proteins (NEDD9/HEF1/CAS-L, BCAR1/p130Cas, EFSSIN, and HEPL/CASS4) regulate cell migration, division and survival, and are often deregulated in cancer...
  9. Malleter M, Jacquot C, Rousseau B, Tomasoni C, Juge M, Pineau A, et al. miRNAs, a potential target in the treatment of Non-Small-Cell Lung Carcinomas. Gene. 2012;506:355-9 pubmed publisher
    ..miRNA is implicated in cancer and may be implicated in NSCLC. Our team has been working on two genes HEF1, a gene implicated in different functions of cell cycle and B2, a large non-coding RNA (nc RNA)...
  10. Kondo S, Iwata S, Yamada T, Inoue Y, Ichihara H, Kichikawa Y, et al. Impact of the integrin signaling adaptor protein NEDD9 on prognosis and metastatic behavior of human lung cancer. Clin Cancer Res. 2012;18:6326-38 pubmed publisher
    ..b>NEDD9 is an integrin signaling adaptor protein composed of multiple domains serving as substrate for a variety of ..
  11. Ohashi Y, Tachibana K, Kamiguchi K, Fujita H, Morimoto C. T cell receptor-mediated tyrosine phosphorylation of Cas-L, a 105-kDa Crk-associated substrate-related protein, and its association of Crk and C3G. J Biol Chem. 1998;273:6446-51 pubmed
    ..Taken together, the present study indicates a novel signaling pathway mediated by tyrosine-phosphorylated Cas-L upon the TCR/CD3 stimulation. ..
  12. Tachibana K, Urano T, Fujita H, Ohashi Y, Kamiguchi K, Iwata S, et al. Tyrosine phosphorylation of Crk-associated substrates by focal adhesion kinase. A putative mechanism for the integrin-mediated tyrosine phosphorylation of Crk-associated substrates. J Biol Chem. 1997;272:29083-90 pubmed
    ..These findings strongly suggest that FAK initiates integrin-mediated tyrosine phosphorylation of Cas proteins; then, Src family tyrosine kinases, which are recruited to phosphorylated Cas and FAK, further phosphorylate Cas proteins. ..
  13. Sattler M, Salgia R, Shrikhande G, Verma S, Uemura N, Law S, et al. Differential signaling after beta1 integrin ligation is mediated through binding of CRKL to p120(CBL) and p110(HEF1). J Biol Chem. 1997;272:14320-6 pubmed
    ..However, in the lymphoid H9 cell line, the major tyrosine-phosphorylated CRKL-binding protein was p110(HEF1). In both cases, this binding was mediated by the CRKL SH2 domain...
  14. Minegishi M, Tachibana K, Sato T, Iwata S, Nojima Y, Morimoto C. Structure and function of Cas-L, a 105-kD Crk-associated substrate-related protein that is involved in beta 1 integrin-mediated signaling in lymphocytes. J Exp Med. 1996;184:1365-75 pubmed
    ..Furthermore, we demonstrate that integrin/ligand binding results in the recruitment of Crk, Nck, and SHPTP2 to pp105. These findings further define the roles of pp105/Cas-L and pp125FAK in the integrin-mediated signaling pathways. ..
  15. Li Y, Grupe A, Rowland C, Holmans P, Segurado R, Abraham R, et al. Evidence that common variation in NEDD9 is associated with susceptibility to late-onset Alzheimer's and Parkinson's disease. Hum Mol Genet. 2008;17:759-67 pubmed
    ..We identified a SNP within a putative transcription factor binding site in the NEDD9 gene (neural precursor cell expressed, developmentally down-regulated), that shows good evidence of association ..
  16. Lucas J, Salimath B, Slomiany M, Rosenzweig S. Regulation of invasive behavior by vascular endothelial growth factor is HEF1-dependent. Oncogene. 2010;29:4449-59 pubmed publisher
    ..A cluster of proteins involved in cell migration and invasion, including the p130Cas paralog, human enhancer of filamentation 1 (HEF1/Cas-L/Nedd9) was identified...
  17. Kong C, Wang C, Wang L, Ma M, Niu C, Sun X, et al. NEDD9 is a positive regulator of epithelial-mesenchymal transition and promotes invasion in aggressive breast cancer. PLoS ONE. 2011;6:e22666 pubmed publisher
    ..b>NEDD9/HEF1/Cas-L is a member of the Cas protein family and was identified as a metastasis marker in multiple cancer ..
  18. O Neill G, Seo S, Serebriiskii I, Lessin S, Golemis E. A new central scaffold for metastasis: parsing HEF1/Cas-L/NEDD9. Cancer Res. 2007;67:8975-9 pubmed
    ..Recently, changes in expression of the scaffold protein HEF1/CAS-L/NEDD9 were found to be a potent prometastatic stimulus in melanoma and other cancers...
  19. O Neill G, Golemis E. Proteolysis of the docking protein HEF1 and implications for focal adhesion dynamics. Mol Cell Biol. 2001;21:5094-108 pubmed
    ..The focal adhesion-associated docking protein HEF1 is cleaved by caspases during both mitosis and apoptosis...
  20. Law S, O Neill G, Fashena S, Einarson M, Golemis E. The docking protein HEF1 is an apoptotic mediator at focal adhesion sites. Mol Cell Biol. 2000;20:5184-95 pubmed
    HEF1 (human enhancer of filamentation 1) is a member of a docking protein family that includes p130(Cas) and Efs...
  21. Zhang Z, Hernandez Lagunas L, Horne W, Baron R. Cytoskeleton-dependent tyrosine phosphorylation of the p130(Cas) family member HEF1 downstream of the G protein-coupled calcitonin receptor. Calcitonin induces the association of HEF1, paxillin, and focal adhesion kinase. J Biol Chem. 1999;274:25093-8 pubmed
    b>HEF1 is a recently described p130(Cas)-like docking protein that contains one SH3 domain and multiple SH2 binding motifs. In B cells, HEF1 is phosphorylated by a cytoskeleton-dependent mechanism that is triggered by integrin ligation...
  22. Law S, Zhang Y, Klein Szanto A, Golemis E. Cell cycle-regulated processing of HEF1 to multiple protein forms differentially targeted to multiple subcellular compartments. Mol Cell Biol. 1998;18:3540-51 pubmed
    b>HEF1, p130(Cas), and Efs/Sin constitute a family of multidomain docking proteins that have been implicated in coordinating the regulation of cell adhesion...
  23. Zhou Z, Tang M, Liu Y, Zhang Z, Lu R, Lu J. Apigenin inhibits cell proliferation, migration, and invasion by targeting Akt in the A549 human lung cancer cell line. Anticancer Drugs. 2017;28:446-456 pubmed publisher
    ..also inhibited its downstream gene expression of matrix metalloproteinases-9, glycogen synthase kinase-3β, and HEF1. Together, APG is a new inhibitor of Akt in lung cancer and a potential natural compound for cancer chemoprevention. ..
  24. Li P, Zhou H, Zhu X, Ma G, Liu C, Lin B, et al. High expression of NEDD9 predicts adverse outcomes of colorectal cancer patients. Int J Clin Exp Pathol. 2014;7:2565-70 pubmed
    b>NEDD9, a member of Crk-associated substrate (CAS) family, is highly expressed in multiple cancer types and involved cancer cell adhesion, migration, invasion. The prognostic value of NEDD9 has not been evaluated before...
  25. Nuñez J, Bai L, Harrington L, Hinder T, Doudna J. CRISPR Immunological Memory Requires a Host Factor for Specificity. Mol Cell. 2016;62:824-833 pubmed publisher
    ..To generate immunological memory, the Cas1-Cas2 protein complex captures 30-40 base pair segments of foreign DNA and catalyzes their integration into the host ..
  26. Miao Y, Cui L, Chen Z, Zhang L. Gene expression profiling of DMU-212-induced apoptosis and anti-angiogenesis in vascular endothelial cells. Pharm Biol. 2016;54:660-6 pubmed publisher
    ..and SPRY4), chromosome organization (HIST1H2BH and GEM), cell migration and angiogenesis (ERRFI1, HBEGF, and NEDD9), and apoptosis (TNFSF15, TNFRSF9, CD274, BCL2L11, BIRC3, TNFAIP3, and TIFA), as well as other genes with unknown ..
  27. Redding S, Sternberg S, Marshall M, Gibb B, Bhat P, Guegler C, et al. Surveillance and Processing of Foreign DNA by the Escherichia coli CRISPR-Cas System. Cell. 2015;163:854-65 pubmed publisher alternative pathway that recruits a nuclease-inactive Cas3 through a mechanism that is dependent on the Cas1 and Cas2 proteins...
  28. Hickman A, Dyda F. The casposon-encoded Cas1 protein from Aciduliprofundum boonei is a DNA integrase that generates target site duplications. Nucleic Acids Res. 2015;43:10576-87 pubmed publisher
    ..Two CRISPR-associated proteins, Cas1 and Cas2, are required for the acquisition step of adaptation, in which fragments of foreign DNA are incorporated into the ..
  29. Dai J, Van Wie P, Fai L, Kim D, Wang L, Poyil P, et al. Downregulation of NEDD9 by apigenin suppresses migration, invasion, and metastasis of colorectal cancer cells. Toxicol Appl Pharmacol. 2016;311:106-112 pubmed publisher
    ..Neural precursor cell expressed developmentally downregulated 9 (NEDD9) is a multi-domain scaffolding protein of the Cas family which has been shown to correlate with cancer metastasis ..
  30. Santos L, Blair D, Kumari S, Cammer M, Iskratsch T, Herbin O, et al. Actin polymerization-dependent activation of Cas-L promotes immunological synapse stability. Immunol Cell Biol. 2016;94:981-993 pubmed publisher
    ..Our studies highlight the importance of mechanotransduction processes in T-cell-mediated immune responses. ..
  31. Chang J, Gao F, Zhao G, Zhang G. Expression and clinical significance of NEDD9 in lung tissues. Med Oncol. 2012;29:2654-60 pubmed publisher
    Neural precursor cell expressed, developmentally downregulated 9 (NEDD9) acts as a scaffold protein and belongs to a family of CAS (Crk-associated substrate) that regulates protein complexes controlling invasion and differentiation...
  32. Silas S, Mohr G, Sidote D, Markham L, Sanchez Amat A, Bhaya D, et al. Direct CRISPR spacer acquisition from RNA by a natural reverse transcriptase-Cas1 fusion protein. Science. 2016;351:aad4234 pubmed publisher
    CRISPR systems mediate adaptive immunity in diverse prokaryotes. CRISPR-associated Cas1 and Cas2 proteins have been shown to enable adaptation to new threats in type I and II CRISPR systems by the acquisition of short segments of DNA (..
  33. Shipman S, Nivala J, Macklis J, Church G. Molecular recordings by directed CRISPR spacer acquisition. Science. 2016;353:aaf1175 pubmed publisher
    ..We then applied directed evolution so as to alter the recognition of a protospacer adjacent motif by the Cas1-Cas2 complex, which enabled recording in two modes simultaneously...
  34. Cote J, Charest A, Wagner J, Tremblay M. Combination of gene targeting and substrate trapping to identify substrates of protein tyrosine phosphatases using PTP-PEST as a model. Biochemistry. 1998;37:13128-37 pubmed
    ..through its first proline-rich sequence 332PPKPPR337, interacts with other members of the p130(Cas) family (Hef1 and Sin) via their SH3 domain in vitro...
  35. Kyono W, De Jong R, Park R, Liu Y, Heisterkamp N, Groffen J, et al. Differential interaction of Crkl with Cbl or C3G, Hef-1, and gamma subunit immunoreceptor tyrosine-based activation motif in signaling of myeloid high affinity Fc receptor for IgG (Fc gamma RI). J Immunol. 1998;161:5555-63 pubmed
  36. Han R, Sun Q, Wu J, Zheng P, Zhao G. Sodium Butyrate Upregulates miR-203 Expression to Exert Anti-Proliferation Effect on Colorectal Cancer Cells. Cell Physiol Biochem. 2016;39:1919-1929 pubmed
    ..We conducted qRT-PCR and Western blotting assays to evaluate the effects of NaBt on the expression of miR-203 and NEDD9 in HT-29 and Caco-2 cell lines...
  37. Ferriero G, Kristensen M, Invernizzi M, Salgovic L, Bravini E, Sartorio F, et al. Psychometric properties of the Cumulated Ambulation Score: a systematic review. Eur J Phys Rehabil Med. 2018;54:766-771 pubmed publisher
    ..Two versions of CAS (CAS1 and the more detailed CAS2 version) and two different methods to calculate the total score (1-day and 3-day CAS) were used in the selected ..
  38. Rollins M, Chowdhury S, Carter J, Golden S, Wilkinson R, Bondy Denomy J, et al. Cas1 and the Csy complex are opposing regulators of Cas2/3 nuclease activity. Proc Natl Acad Sci U S A. 2017;114:E5113-E5121 pubmed publisher
    ..e., Cas2/3) for target degradation...
  39. Jung T, Park K, An Y, Schulga A, Deyev S, Jung J, et al. Structural features of Cas2 from Thermococcus onnurineus in CRISPR-cas system type IV. Protein Sci. 2016;25:1890-7 pubmed publisher
    ..Cas1 and Cas2 are highly conserved components that play an essential part in the adaptation stage of all CRISPR-Cas systems...
  40. Lee H, Zhou Y, Taylor D, Sashital D. Cas4-Dependent Prespacer Processing Ensures High-Fidelity Programming of CRISPR Arrays. Mol Cell. 2018;70:48-59.e5 pubmed publisher
    ..Here we show that Bacillus halodurans type I-C Cas4 is required for efficient prespacer processing prior to Cas1-Cas2-mediated integration...
  41. Wilkinson M, Nakatani Y, Staals R, Kieper S, Opel Reading H, McKenzie R, et al. Structural plasticity and in vivo activity of Cas1 from the type I-F CRISPR-Cas system. Biochem J. 2016;473:1063-72 pubmed publisher
    ..There are many diverse types of CRISPR-Cas systems, including type I-F systems, which are typified by a unique Cas1-Cas2-3 adaptation complex...
  42. Zhou S, Xu M, Shen J, Liu X, Chen M, Cai X. Overexpression of NEDD9 promotes cell invasion and metastasis in hepatocellular carcinoma. Clin Res Hepatol Gastroenterol. 2017;41:677-686 pubmed publisher
    Neural precursor cell expressed, developmentally downregulated 9 (NEDD9), is a focal adhesion scaffold protein which has been associated with metastasis in several cancers. Recent study found that NEDD9 expression was upregulated in HCC...
  43. Yin Y, Sui C, Meng F, Ma P, Jiang Y. The omega-3 polyunsaturated fatty acid docosahexaenoic acid inhibits proliferation and progression of non-small cell lung cancer cells through the reactive oxygen species-mediated inactivation of the PI3K /Akt pathway. Lipids Health Dis. 2017;16:87 pubmed publisher
    ..apoptosis associated protein (caspase-3, PARP,Bax,Bcl-2 and survivin) and metastases associated proteins including HEF1, MMP9 and VEGF were detected by Western blot, and the same method was used in the expression of PI3K and Akt...
  44. Zhang Q, Wang H, Ma Y, Zhang J, He X, Ma J, et al. Overexpression of Nedd9 is a prognostic marker of human gastric cancer. Med Oncol. 2014;31:33 pubmed publisher
    ..the expression and prognostic significance of neural precursor cell-expressed, developmentally downregulated 9 (Nedd9) in patients with gastric cancer...
  45. Liu Y, Wang D, Zhao K, Zhu J, Yin H, Wei Y, et al. NEDD9 overexpression correlates with poor prognosis in gastric cancer. Tumour Biol. 2014;35:6351-6 pubmed publisher
    In this study, the expression of neural precursor cell expressed developmentally downregulated 9 (NEDD9) in benign and malignant gastric tissues was investigated, and the significance of NEDD9 in gastric cancer prognosis was explored...
  46. Hamze Komaiha O, Sarr S, Arlot Bonnemains Y, Samuel D, Gassama Diagne A. SHIP2 Regulates Lumen Generation, Cell Division, and Ciliogenesis through the Control of Basolateral to Apical Lumen Localization of Aurora A and HEF 1. Cell Rep. 2016;17:2738-2752 pubmed publisher
    ..SHIP2 binds Aurora A kinase and the scaffolding protein HEF1 and promotes their basolateral localization at the expense of their luminal expression connected with cilia ..
  47. Huang Q, Luo H, Liu M, Zeng J, Abdalla A, Duan X, et al. The effect of Mycobacterium tuberculosis CRISPR-associated Cas2 (Rv2816c) on stress response genes expression, morphology and macrophage survival of Mycobacterium smegmatis. Infect Genet Evol. 2016;40:295-301 pubmed publisher
    ..The CRISPR-associated Cas2 is an endoribonuclease required for the acquisition of new spacers...
  48. Zhu D, Yang X, He Y, Zhou W, Song X, Wang J, et al. Comparative genomic analysis identifies structural features of CRISPR-Cas systems in Riemerella anatipestifer. BMC Genomics. 2016;17:689 pubmed publisher
    ..Only three cas genes (cas1, cas2 and cas9) were located upstream of CRISPR1...
  49. Herbin O, Regelmann A, Ramkhelawon B, Weinstein E, Moore K, Alexandropoulos K. Monocyte Adhesion and Plaque Recruitment During Atherosclerosis Development Is Regulated by the Adapter Protein Chat-H/SHEP1. Arterioscler Thromb Vasc Biol. 2016;36:1791-801 pubmed publisher
    ..We previously showed that the hematopoietic cell-specific adaptor protein Cas- and Hef1-associated signal transducer hematopoietic isoform (Chat-H)/SHEP1 regulated lymphocyte adhesion and migration...
  50. Zheng H, Wu J, Shi J, Lu C, Wang Y, Sun Q, et al. miR-125a-5p upregulation suppresses the proliferation and induces the cell apoptosis of lung adenocarcinoma by targeting NEDD9. Oncol Rep. 2017;38:1790-1796 pubmed publisher
    ..the mRNA 3' untranslated region (UTR) of neural precursor cell expressed, developmentally downregulated 9 (NEDD9)...
  51. Wei Y, Terns M. CRISPR Outsourcing: Commissioning IHF for Site-Specific Integration of Foreign DNA at the CRISPR Array. Mol Cell. 2016;62:803-804 pubmed publisher
    ..2016) report that site-specific integration of foreign DNA into CRISPR loci by the Cas1-Cas2 integrase complex is promoted by a host factor, IHF (integration host factor), that binds and bends CRISPR leader ..
  52. Smargon A, Cox D, Pyzocha N, Zheng K, Slaymaker I, Gootenberg J, et al. Cas13b Is a Type VI-B CRISPR-Associated RNA-Guided RNase Differentially Regulated by Accessory Proteins Csx27 and Csx28. Mol Cell. 2017;65:618-630.e7 pubmed publisher
    ..sequence database mining approach, we identify two class 2 CRISPR-Cas systems (subtype VI-B) that lack Cas1 and Cas2 and encompass a single large effector protein, Cas13b, along with one of two previously uncharacterized associated ..
  53. Moore F, Osmundson E, Koblinski J, Pugacheva E, Golemis E, Ray D, et al. The WW-HECT protein Smurf2 interacts with the Docking Protein NEDD9/HEF1 for Aurora A activation. Cell Div. 2010;5:22 pubmed publisher
    The multi-functional adaptor protein NEDD9/HEF1/Cas-L regulates cell motility, invasion and cell cycle progression, and plays key roles in cancer progression and metastasis...
  54. Musharova O, Klimuk E, Datsenko K, Metlitskaya A, Logacheva M, Semenova E, et al. Spacer-length DNA intermediates are associated with Cas1 in cells undergoing primed CRISPR adaptation. Nucleic Acids Res. 2017;45:3297-3307 pubmed publisher
    ..A complex of Cas1 and Cas2 proteins is capable of inserting new spacers in the CRISPR array...
  55. Bradbury P, Mahmassani M, Zhong J, Turner K, Paul A, Verrills N, et al. PP2A phosphatase suppresses function of the mesenchymal invasion regulator NEDD9. Biochim Biophys Acta. 2012;1823:290-7 pubmed publisher active adhesion turnover and a polarized actin cytoskeleton, is critically regulated by the adaptor protein NEDD9/HEF1/Cas-L...
  56. Hivert V, Pierre J, Raingeaud J. Phosphorylation of human enhancer of filamentation (HEF1) on serine 369 induces its proteasomal degradation. Biochem Pharmacol. 2009;78:1017-25 pubmed publisher
    Human enhancer of filamentation 1 (HEF1) is a multi-domain docking protein of the p130 Cas family...
  57. Merrill R, See A, Wertheim M, Clagett Dame M. Crk-associated substrate (Cas) family member, NEDD9, is regulated in human neuroblastoma cells and in the embryonic hindbrain by all-trans retinoic acid. Dev Dyn. 2004;231:564-75 pubmed
    ..substrate (Cas) family member, neural precursor cell-expressed, developmentally down-regulated gene 9 (NEDD9) also called the human enhancer of filamentation (HEF1)...
  58. Zhang Y, Lindblom T, Chang A, Sudol M, Sluder A, Golemis E. Evidence that dim1 associates with proteins involved in pre-mRNA splicing, and delineation of residues essential for dim1 interactions with hnRNP F and Npw38/PQBP-1. Gene. 2000;257:33-43 pubmed
    ..These results parallel the arrest phenotypes associated with global disruption of zygotic gene expression, suggesting that Dim1 proteins maintain an essential function in gene expression in higher eukaryotes. ..
  59. Liu J, Rao Y, Cheung M, Hui M, Wu M, Chan L, et al. Asymmetric localization of DLC1 defines avian trunk neural crest polarity for directional delamination and migration. Nat Commun. 2017;8:1185 pubmed publisher
    ..Importantly, the association of DLC1 with NEDD9 is crucial for its asymmetric localization and differential RHOA activity...
  60. Pallarés V, Hoyos M, Chillón M, Barragan E, Conde M, Llop M, et al. NEDD9, an independent good prognostic factor in intermediate-risk acute myeloid leukemia patients. Oncotarget. 2017;8:76003-76014 pubmed publisher
    ..Nevertheless, previous work has established an important role for the CAS family members NEDD9 or BCAR1 in the migratory and dissemination capacities of myeloid cells...
  61. Zhong J, Bach C, Shum M, O Neill G. NEDD9 regulates 3D migratory activity independent of the Rac1 morphology switch in glioma and neuroblastoma. Mol Cancer Res. 2014;12:264-73 pubmed publisher
    ..g., rounded vs. elongated). The adhesion protein NEDD9 has the potential to regulate the switch between elongated and rounded morphologies; therefore, its role was ..
  62. Gomez Smith M, Janik R, Adams C, Lake E, Thomason L, Jeffers M, et al. Reduced Cerebrovascular Reactivity and Increased Resting Cerebral Perfusion in Rats Exposed to a Cafeteria Diet. Neuroscience. 2018;371:166-177 pubmed publisher
    ..Resting cerebral perfusion and cerebrovascular reactivity was quantified using continuous arterial spin labeling (CASL) magnetic resonance imaging (MRI)...
  63. Ibrahim R, Lemoine A, Bertoglio J, Raingeaud J. Human enhancer of filamentation 1-induced colorectal cancer cell migration: Role of serine phosphorylation and interaction with the breast cancer anti-estrogen resistance 3 protein. Int J Biochem Cell Biol. 2015;64:45-57 pubmed publisher
    Human enhancer of filamentation 1 (HEF1) is a member of the p130Cas family of docking proteins involved in integrin-mediated cytoskeleton reorganization associated with cell migration...
  64. Krupovic M, Beguin P, Koonin E. Casposons: mobile genetic elements that gave rise to the CRISPR-Cas adaptation machinery. Curr Opin Microbiol. 2017;38:36-43 pubmed publisher
    ..Comparison of the mechanistic features of the reactions catalyzed by the casposase and the Cas1-Cas2 heterohexamer, the CRISPR integrase, reveals close similarity but also important differences that explain the ..
  65. Juszczak G, Stankiewicz A. Glucocorticoids, genes and brain function. Prog Neuropsychopharmacol Biol Psychiatry. 2018;82:136-168 pubmed publisher
    ..Other genes are involved in the regulation of metabolism (Gpd1, Aldoc and Pdk4), actin cytoskeleton (Myh2, Nedd9, Mical2, Rhou, Arl4d, Osbpl3, Arhgef3, Sdc4, Rdx, Wipf3, Chst1 and Hepacam), autophagy (Eva1a and Plekhf1), ..
  66. Bradbury P, Turner K, Mitchell C, Griffin K, Middlemiss S, Lau L, et al. The focal adhesion targeting domain of p130Cas confers a mechanosensing function. J Cell Sci. 2017;130:1263-1273 pubmed publisher
    ..To determine the role of the FAT domain in these proteins, we compared wild-type exogenous NEDD9 with a hybrid construct in which the NEDD9 FAT domain had been exchanged for the p130Cas (also known as BCAR1) FAT ..
  67. Iwata S, Ohashi Y, Kamiguchi K, Morimoto C. Beta 1-integrin-mediated cell signaling in T lymphocytes. J Dermatol Sci. 2000;23:75-86 pubmed
    ..Taken together, Cas-L might be the bi-modal docking protein that assembles the signals through beta1-integrins and TCR/CD3, and participates in a variety of T-cell functions. ..
  68. Ohashi Y, Iwata S, Kamiguchi K, Morimoto C. Tyrosine phosphorylation of Crk-associated substrate lymphocyte-type is a critical element in TCR- and beta 1 integrin-induced T lymphocyte migration. J Immunol. 1999;163:3727-34 pubmed
    ..These data strongly suggest that Cas-L is a key molecule in T cell migration induced by the ligation of CD3 and beta 1 integrins and that tyrosine phosphorylation of Cas-L is essential for T cell migration. ..
  69. Zhang W, Li S, Zhao Y, Guo N, Li Y. [Knockdown of NEDD9 inhibits the proliferation, invasion and migration of esophageal carcinoma EC109 cells]. Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2016;32:1662-1666 pubmed
    Objective To observe the expression of the neural precursor cell expressed, developmentally down-regulated 9 (NEDD9) in esophageal cancer, to investigate the impact of decreased expression of NEDD9 on invasion and migration, and to ..
  70. Fu Y, He F, Tang N, Tam C, Lui V, Chiu H, et al. NEDD9 gene polymorphism influences the risk of Alzheimer disease and cognitive function in Chinese older persons. Alzheimer Dis Assoc Disord. 2012;26:88-90 pubmed publisher
    Neural precursor cell expressed, developmentally down-regulated (NEDD9) gene was a new candidate risk gene for Alzheimer disease (AD)...
  71. Speranza M, Frattini V, Pisati F, Kapetis D, Porrati P, Eoli M, et al. NEDD9, a novel target of miR-145, increases the invasiveness of glioblastoma. Oncotarget. 2012;3:723-34 pubmed
    ..We focused our attention on HEF1/Cas-L/NEDD9, a scaffold protein involved in invasion in several types of cancer...
  72. Wang Y, Bi L, Wang H, Li Y, Di Q, Xu W, et al. NEDD9 rs760678 polymorphism and the risk of Alzheimer's disease: a meta-analysis. Neurosci Lett. 2012;527:121-5 pubmed publisher
    The NEDD9 rs760678 polymorphism has been extensively investigated for association to Alzheimer's disease (AD), however, results of different studies have been inconsistent...
  73. Xue Y, Sheng Y, Liu Z, Wei Z, Cao H, Wu Y, et al. Expression of NEDD9 in pancreatic ductal adenocarcinoma and its clinical significance. Tumour Biol. 2013;34:895-9 pubmed publisher
    The aim of this study was to investigate the expression and prognostic significance of NEDD9 in pancreatic ductal adenocarcinoma (PDA)...
  74. Guo W, Ren D, Chen X, Tu X, Huang S, Wang M, et al. HEF1 promotes epithelial mesenchymal transition and bone invasion in prostate cancer under the regulation of microRNA-145. J Cell Biochem. 2013;114:1606-15 pubmed publisher
    ..In the present study, we constructed a luciferase reporter system and identified HEF1 as a direct target of miR-145...
  75. Ice R, McLaughlin S, Livengood R, Culp M, Eddy E, Ivanov A, et al. NEDD9 depletion destabilizes Aurora A kinase and heightens the efficacy of Aurora A inhibitors: implications for treatment of metastatic solid tumors. Cancer Res. 2013;73:3168-80 pubmed publisher
    ..Here, we show that NEDD9, a known activator of AURKA, is directly involved in AURKA stability...
  76. Jin Y, Li F, Zheng C, Wang Y, Fang Z, Guo C, et al. NEDD9 promotes lung cancer metastasis through epithelial-mesenchymal transition. Int J Cancer. 2014;134:2294-304 pubmed publisher
    ..The scaffolding protein neural precursor cell expressed, developmentally down-regulated 9 (NEDD9) has been identified as a pro-metastasis gene in several types of cancers including melanoma and breast cancer...
  77. Karabulut M, Alis H, Afsar C, Karabulut S, Kocataş A, Oguz H, et al. Serum neural precursor cell-expressed, developmentally down regulated 9 (NEDD9) level may have a prognostic role in patients with gastric cancer. Biomed Pharmacother. 2015;73:140-6 pubmed publisher
    Neural precursor cell-expressed, developmentally down regulated 9 (NEDD9), a member of Crk-associated substrate (CAS) family, is highly expressed in multiple cancer types and involved in cancer cell adhesion, migration and invasion...
  78. Wang Z, Shen M, Lu P, Li X, Zhu S, Yue S. NEDD9 may regulate hepatocellular carcinoma cell metastasis by promoting epithelial-mesenchymal-transition and stemness via repressing Smad7. Oncotarget. 2017;8:1714-1724 pubmed publisher
    Overexpression of neural precursor cell expressed, developmentally downregulated 9 (NEDD9) is a prognostic marker of many cancers, including hepatocellular carcinoma (HCC). However, the functions and mechanisms of NEDD9 are unclear...
  79. Sima N, Cheng X, Ye F, Ma D, Xie X, Lu W. The overexpression of scaffolding protein NEDD9 promotes migration and invasion in cervical cancer via tyrosine phosphorylated FAK and SRC. PLoS ONE. 2013;8:e74594 pubmed publisher
    b>NEDD9, a focal adhesion scaffolding protein, has been recently proposed to regulate invasion and metastasis in some cancer types, but unknown in cervical cancer...
  80. Shah V, Aiken C. Gene expression analysis of a panel of cell lines that differentially restrict HIV-1 CA mutants infection in a cyclophilin a-dependent manner. PLoS ONE. 2014;9:e92724 pubmed publisher
    ..Our study provides gene expression data that may be useful for future efforts to identify the putative CypA-dependent HIV-1 restriction factor and in studies of other cell-specific phenotypes. ..
  81. Han T, Yi X, Liu B, Ke M, Li Y. MicroRNA-145 suppresses cell proliferation, invasion and migration in pancreatic cancer cells by targeting NEDD9. Mol Med Rep. 2015;11:4115-20 pubmed publisher
    ..Neural precursor cell expressed, developmentally down‑regulated 9 (NEDD9) has been identified as a novel potential miR‑145 target using bioinformatics...
  82. Stajduhar E, Sedic M, Lenicek T, Radulović P, Kerenji A, Kruslin B, et al. Expression of growth hormone receptor, plakoglobin and NEDD9 protein in association with tumour progression and metastasis in human breast cancer. Tumour Biol. 2014;35:6425-34 pubmed publisher, we investigated the expression of proteins involved in cell signalling (growth hormone receptor (GHR) and NEDD9) and cell-cell adhesion (plakoglobin) in epithelial and stromal compartments of primary ductal invasive breast ..