Nedd8

Summary

Gene Symbol: Nedd8
Description: neural precursor cell expressed, developmentally down-regulated 8
Alias: NEDD-8, NEDD8, neddylin, ubiquitin-like protein Nedd8
Species: human
Products:     Nedd8

Top Publications

  1. Wada H, Yeh E, Kamitani T. A dominant-negative UBC12 mutant sequesters NEDD8 and inhibits NEDD8 conjugation in vivo. J Biol Chem. 2000;275:17008-15 pubmed
    b>NEDD8, a novel ubiquitin-like protein, has been shown to conjugate to proteins in a manner analogous to ubiquitination and sentrinization. Recently, human UBC12 was identified as a putative NEDD8 conjugation enzyme (E2)...
  2. Kurz T, Ozlu N, Rudolf F, O Rourke S, Luke B, Hofmann K, et al. The conserved protein DCN-1/Dcn1p is required for cullin neddylation in C. elegans and S. cerevisiae. Nature. 2005;435:1257-61 pubmed
    ..Cycles of covalent modification of cullins by the ubiquitin-like molecule Nedd8 (neddylation) and removal of Nedd8 by the COP9 signalosome (deneddylation) positively regulate E3 ligase activity...
  3. Osaka F, Kawasaki H, Aida N, Saeki M, Chiba T, Kawashima S, et al. A new NEDD8-ligating system for cullin-4A. Genes Dev. 1998;12:2263-8 pubmed
    b>NEDD8 is a ubiquitin (Ub)-like protein. Here we report a novel ubiquitinylation-related pathway for modification by NEDD8...
  4. Kumar S, Yoshida Y, Noda M. Cloning of a cDNA which encodes a novel ubiquitin-like protein. Biochem Biophys Res Commun. 1993;195:393-9 pubmed
    ..These data suggest that like ubiquitin, the product of this gene may play some essential role in eukaryotic cellular metabolism. ..
  5. Gong L, Yeh E. Identification of the activating and conjugating enzymes of the NEDD8 conjugation pathway. J Biol Chem. 1999;274:12036-42 pubmed
    b>NEDD8 is a ubiquitin-like molecule that can be covalently conjugated to a limited number of cellular proteins, such as Cdc53/cullin...
  6. Skaar J, Florens L, Tsutsumi T, Arai T, Tron A, Swanson S, et al. PARC and CUL7 form atypical cullin RING ligase complexes. Cancer Res. 2007;67:2006-14 pubmed
    ..PARC binds RBX1 and is covalently modified by NEDD8, defining PARC as a true cullin...
  7. Enchev R, Scott D, da Fonseca P, Schreiber A, Monda J, Schulman B, et al. Structural basis for a reciprocal regulation between SCF and CSN. Cell Rep. 2012;2:616-27 pubmed publisher
    Skp1-Cul1-Fbox (SCF) E3 ligases are activated by ligation to the ubiquitin-like protein Nedd8, which is reversed by the deneddylating Cop9 signalosome (CSN). However, CSN also promotes SCF substrate turnover through unknown mechanisms...
  8. Watson I, Irwin M, Ohh M. NEDD8 pathways in cancer, Sine Quibus Non. Cancer Cell. 2011;19:168-76 pubmed publisher
    There are 17 known ubiquitin-like proteins (UBLs) from nine phylogenetically distinct classes (NEDD8, SUMO, ISG15, FUB1, FAT10, Atg8, Atg12, Urm1, and UFM1) that have been identified to conjugate to substrates in a manner analogous to ..
  9. Stickle N, Chung J, Klco J, Hill R, Kaelin W, Ohh M. pVHL modification by NEDD8 is required for fibronectin matrix assembly and suppression of tumor development. Mol Cell Biol. 2004;24:3251-61 pubmed
    ..Here, we report that a ubiquitin-like molecule called NEDD8 covalently modifies pVHL...

More Information

Publications68

  1. Gastaldello S, Callegari S, Coppotelli G, Hildebrand S, Song M, Masucci M. Herpes virus deneddylases interrupt the cullin-RING ligase neddylation cycle by inhibiting the binding of CAND1. J Mol Cell Biol. 2012;4:242-51 pubmed publisher
    ..domains of the major tegument proteins of herpes viridae encode cysteine proteases with potent ubiquitin and NEDD8-specific deconjugase activity...
  2. Ohki Y, Funatsu N, Konishi N, Chiba T. The mechanism of poly-NEDD8 chain formation in vitro. Biochem Biophys Res Commun. 2009;381:443-7 pubmed publisher
    b>NEDD8 is a small ubiquitin-like protein that modifies target proteins in a reaction similar to ubiquitination...
  3. Liu J, Furukawa M, Matsumoto T, Xiong Y. NEDD8 modification of CUL1 dissociates p120(CAND1), an inhibitor of CUL1-SKP1 binding and SCF ligases. Mol Cell. 2002;10:1511-8 pubmed
    ..Covalent modification of cullins by the ubiquitin-like protein NEDD8 activates cullin ligases through an as yet undefined mechanism...
  4. Emberley E, Mosadeghi R, Deshaies R. Deconjugation of Nedd8 from Cul1 is directly regulated by Skp1-F-box and substrate, and the COP9 signalosome inhibits deneddylated SCF by a noncatalytic mechanism. J Biol Chem. 2012;287:29679-89 pubmed publisher
    COP9 signalosome (CSN) mediates deconjugation of the ubiquitin-like protein Nedd8 from the cullin subunits of SCF and other cullin-RING ubiquitin ligases (CRLs). This process is essential to maintain the proper activity of CRLs in cells...
  5. Read M, Brownell J, Gladysheva T, Hottelet M, Parent L, Coggins M, et al. Nedd8 modification of cul-1 activates SCF(beta(TrCP))-dependent ubiquitination of IkappaBalpha. Mol Cell Biol. 2000;20:2326-33 pubmed
    ..Here we show that Nedd8 modification of the Cul-1 component of SCF(beta(TrCP)) is important for function of SCF(beta(TrCP)) in ..
  6. Kumar S, Tomooka Y, Noda M. Identification of a set of genes with developmentally down-regulated expression in the mouse brain. Biochem Biophys Res Commun. 1992;185:1155-61 pubmed
    ..Their patterns of expression indicate that these genes may play important roles during the embryonic development and differentiation of central nervous system. ..
  7. Saha A, Deshaies R. Multimodal activation of the ubiquitin ligase SCF by Nedd8 conjugation. Mol Cell. 2008;32:21-31 pubmed publisher
    Conjugation of ubiquitin-like protein Nedd8 to cullins (neddylation) is essential for the function of cullin-RING ubiquitin ligases (CRLs). Here, we show that neddylation stimulates CRL activity by multiple mechanisms...
  8. Wimuttisuk W, Singer J. The Cullin3 ubiquitin ligase functions as a Nedd8-bound heterodimer. Mol Biol Cell. 2007;18:899-909 pubmed
    ..attachment of the ubiquitin homologue neural-precursor-cell-expressed and developmentally down regulated 8 (Nedd8) near the C terminus of Cul3...
  9. Gao F, Cheng J, Shi T, Yeh E. Neddylation of a breast cancer-associated protein recruits a class III histone deacetylase that represses NFkappaB-dependent transcription. Nat Cell Biol. 2006;8:1171-7 pubmed
    ..role in ubiquitin-mediated protein degradation through modification of cullins, which are the main substrates for NEDD8 modification. Here, we show that breast cancer-associated protein 3 (BCA3) is a NEDD8 substrate...
  10. Watson I, Blanch A, Lin D, Ohh M, Irwin M. Mdm2-mediated NEDD8 modification of TAp73 regulates its transactivation function. J Biol Chem. 2006;281:34096-103 pubmed
    ..Here, we report that TAp73, but not deltaNp73, is covalently modified by NEDD8 under physiologic conditions in an Mdm2-dependent manner...
  11. Watson I, Li B, Roche O, Blanch A, Ohh M, Irwin M. Chemotherapy induces NEDP1-mediated destabilization of MDM2. Oncogene. 2010;29:297-304 pubmed publisher
    ..Here, we show that the stability of MDM2 is regulated by an ubiquitin-like NEDD8 pathway and identify NEDP1 as a chemotherapy-induced isopeptidase that deneddylates MDM2, resulting in MDM2 ..
  12. Oved S, Mosesson Y, Zwang Y, Santonico E, Shtiegman K, Marmor M, et al. Conjugation to Nedd8 instigates ubiquitylation and down-regulation of activated receptor tyrosine kinases. J Biol Chem. 2006;281:21640-51 pubmed
    ..the ubiquitin ligase of EGFR, namely c-Cbl, also mediates receptor modification with the ubiquitin-like molecule Nedd8. EGF stimulates receptor neddylation, which enhances subsequent ubiquitylation, as well as sorting of EGFR for ..
  13. Liu G, Xirodimas D. NUB1 promotes cytoplasmic localization of p53 through cooperation of the NEDD8 and ubiquitin pathways. Oncogene. 2010;29:2252-61 pubmed publisher
    ..Cytoplasmic p53 is shown to promote apoptosis and inhibit the induction of autophagy. In this study we show that NEDD8 ultimate buster 1 (NUB1), a non-covalent interactor of the Ubl NEDD8 (neural precursor cell expressed, ..
  14. Walden H, Podgorski M, Huang D, Miller D, Howard R, Minor D, et al. The structure of the APPBP1-UBA3-NEDD8-ATP complex reveals the basis for selective ubiquitin-like protein activation by an E1. Mol Cell. 2003;12:1427-37 pubmed
    ..report here the structure of the 120 kDa quaternary complex between human APPBP1-UBA3, a heterodimeric E1, its ubl NEDD8, and ATP...
  15. Gastaldello S, Hildebrand S, Faridani O, Callegari S, Palmkvist M, Di Guglielmo C, et al. A deneddylase encoded by Epstein-Barr virus promotes viral DNA replication by regulating the activity of cullin-RING ligases. Nat Cell Biol. 2010;12:351-61 pubmed publisher
    ..BPLF1 hydrolyses NEDD8 conjugates in vitro, acts as a deneddylase in vivo, binds to cullins and stabilizes CRL substrates...
  16. Mahata B, Sundqvist A, Xirodimas D. Recruitment of RPL11 at promoter sites of p53-regulated genes upon nucleolar stress through NEDD8 and in an Mdm2-dependent manner. Oncogene. 2012;31:3060-71 pubmed publisher
    ..Furthermore, analysis of 36 ActD-induced genes shows that L11 and NEDD8 are global regulators of the p53 activation response...
  17. Bandau S, Knebel A, Gage Z, Wood N, Alexandru G. UBXN7 docks on neddylated cullin complexes using its UIM motif and causes HIF1? accumulation. BMC Biol. 2012;10:36 pubmed publisher
    ..Instead, it relies on the UIM motif in UBXN7 that directly engages the NEDD8 modification on cullins...
  18. Whitby F, Xia G, Pickart C, Hill C. Crystal structure of the human ubiquitin-like protein NEDD8 and interactions with ubiquitin pathway enzymes. J Biol Chem. 1998;273:34983-91 pubmed
    The NEDD8/Rub1 class of ubiquitin-like proteins has been implicated in progression of the cell cycle from G1 into S phase...
  19. Kelsall I, Duda D, Olszewski J, Hofmann K, Knebel A, Langevin F, et al. TRIAD1 and HHARI bind to and are activated by distinct neddylated Cullin-RING ligase complexes. EMBO J. 2013;32:2848-60 pubmed publisher
    ..Cumulatively, our work proposes a conserved mechanism of CRL-induced Ariadne RBR ligase activation and further suggests a reciprocal role of this special class of RBRs as regulators of distinct CRLs. ..
  20. Broemer M, Tenev T, Rigbolt K, Hempel S, Blagoev B, Silke J, et al. Systematic in vivo RNAi analysis identifies IAPs as NEDD8-E3 ligases. Mol Cell. 2010;40:810-22 pubmed publisher
    ..Systematic in vivo RNAi analysis identified three NEDD8-specific isopeptidases that, when knocked down, suppress apoptosis...
  21. Ryu J, Li S, Park H, Park J, Lee B, Chun Y. Hypoxia-inducible factor ? subunit stabilization by NEDD8 conjugation is reactive oxygen species-dependent. J Biol Chem. 2011;286:6963-70 pubmed publisher
    ..b>NEDD8, a member of the ubiquitin-like family, covalently binds to its substrate proteins, and thus, regulates their ..
  22. Li T, Santockyte R, Shen R, Tekle E, Wang G, Yang D, et al. A general approach for investigating enzymatic pathways and substrates for ubiquitin-like modifiers. Arch Biochem Biophys. 2006;453:70-4 pubmed
    ..approach to elucidate the enzymatic pathways and identify target proteins for three UBLs: SUMO-2, SUMO-3, and NEDD8. Expression plasmids containing the cDNAs of Myc/6xHis doubly-tagged processed or non-conjugatable forms of these ..
  23. Leidecker O, Matic I, Mahata B, Pion E, Xirodimas D. The ubiquitin E1 enzyme Ube1 mediates NEDD8 activation under diverse stress conditions. Cell Cycle. 2012;11:1142-50 pubmed publisher
    ..that under stress conditions, the ubiquitin E1 enzyme Ube1 mediates conjugation of the ubiquitin-like molecule NEDD8. Inhibition of the 26S proteasome, heat shock and oxidative stress cause a global increase in NEDDylation...
  24. Groisman R, Polanowska J, Kuraoka I, Sawada J, Saijo M, Drapkin R, et al. The ubiquitin ligase activity in the DDB2 and CSA complexes is differentially regulated by the COP9 signalosome in response to DNA damage. Cell. 2003;113:357-67 pubmed
    ..Knockdown of CSN with RNA interference leads to defects in NER. These results suggest that the distinct UV response of the DDB2 and CSA complexes is involved in diverse mechanisms of NER. ..
  25. Crow A, Hughes R, Taïeb F, Oswald E, Banfield M. The molecular basis of ubiquitin-like protein NEDD8 deamidation by the bacterial effector protein Cif. Proc Natl Acad Sci U S A. 2012;109:E1830-8 pubmed publisher
    ..that interfere with the host cell cycle by catalyzing the deamidation of a specific glutamine residue (Gln40) in NEDD8 and the related protein ubiquitin...
  26. Ferro A, Carvalho A, Teixeira Castro A, Almeida C, Tomé R, Cortes L, et al. NEDD8: a new ataxin-3 interactor. Biochim Biophys Acta. 2007;1773:1619-27 pubmed
    ..Recently, the accumulation of the neural precursor cell expressed developmentally downregulated 8 (NEDD8), a ubiquitin-like protein, in the inclusions of MJD brains was reported...
  27. Huang D, Hunt H, Zhuang M, Ohi M, Holton J, Schulman B. Basis for a ubiquitin-like protein thioester switch toggling E1-E2 affinity. Nature. 2007;445:394-8 pubmed
    ..Here we report the structural analysis of a trapped UBL activation complex for the human NEDD8 pathway, containing NEDD8's heterodimeric E1 (APPBP1-UBA3), two NEDD8s (one thioester-linked to E1, one ..
  28. Calabrese M, Scott D, Duda D, Grace C, Kurinov I, Kriwacki R, et al. A RING E3-substrate complex poised for ubiquitin-like protein transfer: structural insights into cullin-RING ligases. Nat Struct Mol Biol. 2011;18:947-9 pubmed publisher
    ..Here we address how the RING E3 RBX1 positions NEDD8's E2 (UBC12) and substrate (CUL1)...
  29. Liakopoulos D, Büsgen T, Brychzy A, Jentsch S, Pause A. Conjugation of the ubiquitin-like protein NEDD8 to cullin-2 is linked to von Hippel-Lindau tumor suppressor function. Proc Natl Acad Sci U S A. 1999;96:5510-5 pubmed
    ..Here, we show that hCUL-2 is modified by the conserved ubiquitin-like protein NEDD8 and that NEDD8-hCUL-2 conjugates are part of CBCVHL complexes in vivo...
  30. Walden H, Podgorski M, Schulman B. Insights into the ubiquitin transfer cascade from the structure of the activating enzyme for NEDD8. Nature. 2003;422:330-4 pubmed
    ..The Ublp NEDD8 regulates cell division, signalling and embryogenesis...
  31. Tanaka T, Kawashima H, Yeh E, Kamitani T. Regulation of the NEDD8 conjugation system by a splicing variant, NUB1L. J Biol Chem. 2003;278:32905-13 pubmed
    b>NEDD8 is a ubiquitin-like protein that controls vital biological events through its conjugation to target proteins...
  32. Hemelaar J, Borodovsky A, Kessler B, Reverter D, Cook J, Kolli N, et al. Specific and covalent targeting of conjugating and deconjugating enzymes of ubiquitin-like proteins. Mol Cell Biol. 2004;24:84-95 pubmed
    ..To target and identify UBL-modifying enzymes, we produced Nedd8, ISG15, and SUMO-1 in Escherichia coli and equipped them with a C-terminal electrophilic trap (vinyl sulfone [VS]) ..
  33. Reverter D, Wu K, Erdene T, Pan Z, Wilkinson K, Lima C. Structure of a complex between Nedd8 and the Ulp/Senp protease family member Den1. J Mol Biol. 2005;345:141-51 pubmed
    The Nedd8 conjugation pathway is conserved from yeast to humans and is essential in many organisms...
  34. Huang D, Paydar A, Zhuang M, Waddell M, Holton J, Schulman B. Structural basis for recruitment of Ubc12 by an E2 binding domain in NEDD8's E1. Mol Cell. 2005;17:341-50 pubmed
    ..We report here the crystal structure of a complex between the C-terminal domain from NEDD8's heterodimeric E1 (APPBP1-UBA3) and the catalytic core domain of NEDD8's E2 (Ubc12)...
  35. Sakata E, Yamaguchi Y, Miyauchi Y, Iwai K, Chiba T, Saeki Y, et al. Direct interactions between NEDD8 and ubiquitin E2 conjugating enzymes upregulate cullin-based E3 ligase activity. Nat Struct Mol Biol. 2007;14:167-8 pubmed
    ..cullin-1 neddylation is crucial for the activation of SCF ubiquitin E3 ligases, the underlying mechanisms for NEDD8-mediated activation of SCF remain unclear...
  36. Bornstein G, Ganoth D, Hershko A. Regulation of neddylation and deneddylation of cullin1 in SCFSkp2 ubiquitin ligase by F-box protein and substrate. Proc Natl Acad Sci U S A. 2006;103:11515-20 pubmed
    ..ubiquitin protein ligase complexes is stimulated by linkage to cullin of the ubiquitin-like protein Nedd8 ("neddylation")...
  37. Carter S, Bischof O, Dejean A, Vousden K. C-terminal modifications regulate MDM2 dissociation and nuclear export of p53. Nat Cell Biol. 2007;9:428-35 pubmed
    ..Our results suggest that modifications such as sumoylation can regulate the strength of the p53-MDM2 interaction and participate in driving the export of p53. ..
  38. Stanley D, Bartholomeeusen K, Crosby D, Kim D, Kwon E, Yen L, et al. Inhibition of a NEDD8 Cascade Restores Restriction of HIV by APOBEC3G. PLoS Pathog. 2012;8:e1003085 pubmed publisher
    ..least three different Cullin-RING ubiquitin ligases, which must be activated by the small ubiquitin-like protein NEDD8, in order to counteract host cellular restriction factors...
  39. Wu K, Yamoah K, Dolios G, Gan Erdene T, Tan P, Chen A, et al. DEN1 is a dual function protease capable of processing the C terminus of Nedd8 and deconjugating hyper-neddylated CUL1. J Biol Chem. 2003;278:28882-91 pubmed
    b>Nedd8 activates ubiquitination by increasing the efficiency of polyubiquitin chain assembly through its covalent conjugation to cullin molecules...
  40. Abida W, Nikolaev A, Zhao W, Zhang W, Gu W. FBXO11 promotes the Neddylation of p53 and inhibits its transcriptional activity. J Biol Chem. 2007;282:1797-804 pubmed
    ..It has previously been shown that the ubiquitin ligase Mdm2 also promotes the conjugation of Nedd8, a ubiquitin-like protein, to p53, inhibiting its transcriptional activity...
  41. Park Y, Yoon S, Yoon J. TRIP12 functions as an E3 ubiquitin ligase of APP-BP1. Biochem Biophys Res Commun. 2008;374:294-8 pubmed publisher
    The NEDD8 pathway plays an essential role in various physiological processes, such as cell cycle progression and signal transduction...
  42. Souphron J, Waddell M, Paydar A, Tokgöz Gromley Z, Roussel M, Schulman B. Structural dissection of a gating mechanism preventing misactivation of ubiquitin by NEDD8's E1. Biochemistry. 2008;47:8961-9 pubmed publisher
    ..Ubiquitin's Arg 72, which corresponds to Ala72 in the UBL NEDD8, is a key E1 selectivity determinant: swapping ubiquitin and NEDD8 residue 72 identity was shown previously to ..
  43. Yamoah K, Oashi T, Sarikas A, Gazdoiu S, Osman R, Pan Z. Autoinhibitory regulation of SCF-mediated ubiquitination by human cullin 1's C-terminal tail. Proc Natl Acad Sci U S A. 2008;105:12230-5 pubmed publisher
    ..Thus, disruption of ECTD yields in vitro effects that parallel SCF activation by Nedd8 conjugation to CUL1...
  44. Duda D, Borg L, Scott D, Hunt H, Hammel M, Schulman B. Structural insights into NEDD8 activation of cullin-RING ligases: conformational control of conjugation. Cell. 2008;134:995-1006 pubmed publisher
    ..Covalent attachment of the ubiquitin-like protein NEDD8 to a conserved C-terminal domain (ctd) lysine stimulates CRL ubiquitination activity and prevents binding of the ..
  45. Xirodimas D, Saville M, Bourdon J, Hay R, Lane D. Mdm2-mediated NEDD8 conjugation of p53 inhibits its transcriptional activity. Cell. 2004;118:83-97 pubmed
    The only reported role for the conjugation of the NEDD8 ubiquitin-like molecule is control of the activity of SCF ubiquitin ligase complexes...
  46. Shen L, Liu H, Dong C, Xirodimas D, Naismith J, Hay R. Structural basis of NEDD8 ubiquitin discrimination by the deNEDDylating enzyme NEDP1. EMBO J. 2005;24:1341-51 pubmed
    b>NEDD8 (neural precursor cell expressed developmentally downregulated gene 8)-specific protease NEDP1 processes preNEDD8 to its mature form and deconjugates NEDD8 from substrates such as p53 and cullins...
  47. Hjerpe R, Thomas Y, Chen J, Zemla A, Curran S, Shpiro N, et al. Changes in the ratio of free NEDD8 to ubiquitin triggers NEDDylation by ubiquitin enzymes. Biochem J. 2012;441:927-36 pubmed publisher
    ..Ubiquitin modification (ubiquitylation) targets many substrates, often leading to their proteasomal degradation. NEDD8 (neural-precursor-cell-expressed developmentally down-regulated 8) is the UBL most closely related to ubiquitin, ..
  48. Bohnsack R, Haas A. Conservation in the mechanism of Nedd8 activation by the human AppBp1-Uba3 heterodimer. J Biol Chem. 2003;278:26823-30 pubmed
    Human Nedd8-activating enzyme AppBp1-Uba3 was purified to apparent homogeneity from erythrocytes...
  49. Xirodimas D, Sundqvist A, Nakamura A, Shen L, Botting C, Hay R. Ribosomal proteins are targets for the NEDD8 pathway. EMBO Rep. 2008;9:280-6 pubmed publisher
    ..The ubiquitin-like molecule NEDD8 is implicated in the regulation of cell proliferation, viability and development...
  50. Morimoto M, Nishida T, Nagayama Y, Yasuda H. Nedd8-modification of Cul1 is promoted by Roc1 as a Nedd8-E3 ligase and regulates its stability. Biochem Biophys Res Commun. 2003;301:392-8 pubmed
    ..protein expression system, Cul1 was highly neddylated in the cell, suggesting that Roc1 may function as a Nedd8-E3 ligase. However, there is no direct evidence that Roc1 is a Nedd8-E3 in an in vitro enzyme system...
  51. Xie P, Zhang M, He S, Lu K, Chen Y, Xing G, et al. The covalent modifier Nedd8 is critical for the activation of Smurf1 ubiquitin ligase in tumorigenesis. Nat Commun. 2014;5:3733 pubmed publisher
    Neddylation, the covalent attachment of ubiquitin-like protein Nedd8, of the Cullin-RING E3 ligase family regulates their ubiquitylation activity. However, regulation of HECT ligases by neddylation has not been reported to date...
  52. Norman J, Shiekhattar R. Analysis of Nedd8-associated polypeptides: a model for deciphering the pathway for ubiquitin-like modifications. Biochemistry. 2006;45:3014-9 pubmed
    ..Affinity purification of an epitope-tagged Nedd8 allowed the identification of the majority of proteins known to be involved with the neddylation pathway...
  53. Sundqvist A, Liu G, Mirsaliotis A, Xirodimas D. Regulation of nucleolar signalling to p53 through NEDDylation of L11. EMBO Rep. 2009;10:1132-9 pubmed publisher
    ..We have recently shown that RPs are targets for the ubiquitin-like molecule NEDD8, and that NEDDylation protects RPs from destabilization...
  54. den Besten W, Verma R, Kleiger G, Oania R, Deshaies R. NEDD8 links cullin-RING ubiquitin ligase function to the p97 pathway. Nat Struct Mol Biol. 2012;19:511-6, S1 pubmed publisher
    ..UBXD7 and its yeast ortholog, Ubx5, associate only with the active, NEDD8- or Rub1-modified form of cullins...
  55. Morimoto M, Nishida T, Honda R, Yasuda H. Modification of cullin-1 by ubiquitin-like protein Nedd8 enhances the activity of SCF(skp2) toward p27(kip1). Biochem Biophys Res Commun. 2000;270:1093-6 pubmed
    ..Cullin-1, a component of SCF, is modified by ubiquitin-like protein Nedd8. The modification site of cullin-1 was shown to be K696 because the K696R mutant was not modified...
  56. Huang D, Zhuang M, Ayrault O, Schulman B. Identification of conjugation specificity determinants unmasks vestigial preference for ubiquitin within the NEDD8 E2. Nat Struct Mol Biol. 2008;15:280-7 pubmed publisher
    ..12 (Ubc12), which is specific for the neural precursor cell expressed, developmentally down-regulated protein 8 (NEDD8), and does not form a thioester-linked conjugate with ubiquitin...
  57. Hrecka K, Gierszewska M, Srivastava S, Kozaczkiewicz L, Swanson S, Florens L, et al. Lentiviral Vpr usurps Cul4-DDB1[VprBP] E3 ubiquitin ligase to modulate cell cycle. Proc Natl Acad Sci U S A. 2007;104:11778-83 pubmed publisher
  58. Kamitani T, Kito K, Nguyen H, Yeh E. Characterization of NEDD8, a developmentally down-regulated ubiquitin-like protein. J Biol Chem. 1997;272:28557-62 pubmed
    b>NEDD8 is a novel 81 amino acid polypeptide which is 60% identical and 80% homologous to ubiquitin. Northern blot analysis showed that the NEDD8 message was developmentally down-regulated...
  59. Kim A, Bommelje C, Lee B, Yonekawa Y, Choi L, Morris L, et al. SCCRO (DCUN1D1) is an essential component of the E3 complex for neddylation. J Biol Chem. 2008;283:33211-20 pubmed publisher
    Covalent modification of cullins by the ubiquitin-like protein NEDD8 (neddylation) regulates protein ubiquitination by promoting the assembly of cullin-RING ligase E3 complexes...