NDC80

Summary

Gene Symbol: NDC80
Description: NDC80 kinetochore complex component
Alias: HEC, HEC1, HsHec1, KNTC2, TID3, hsNDC80, NDC80 homolog, kinetochore complex component, NDC80 kinetochore complex component homolog, highly expressed in cancer protein, highly expressed in cancer, rich in leucine heptad repeats, kinetochore associated 2, kinetochore protein Hec1, kinetochore-associated protein 2, retinoblastoma-associated protein HEC
Species: human

Top Publications

  1. ncbi Delayed nausea and vomiting continue to reduce patients' quality of life after highly and moderately emetogenic chemotherapy despite antiemetic treatment
    Brigitte Bloechl-Daum
    Department of Clinical Pharmacology, Medical University of Vienna, Vienna, Austria
    J Clin Oncol 24:4472-8. 2006
  2. ncbi Cost-effectiveness analysis of aprepitant in the prevention of chemotherapy-induced nausea and vomiting in Belgium
    Lieven Annemans
    Ghent University, De Pintelaan 185, 9000, Gent, Belgium
    Support Care Cancer 16:905-15. 2008
  3. pmc Semi-solid gels function as physical barriers to human immunodeficiency virus transport in vitro
    Bonnie E Lai
    Department of Biomedical Engineering, Duke University, Durham, NC 27708, USA
    Antiviral Res 88:143-51. 2010
  4. pmc Abnormal kinetochore-generated pulling forces from expressing a N-terminally modified Hec1
    Marta Mattiuzzo
    Institute of Molecular Biology and Pathology, National Research Council of Italy, Rome, Italy
    PLoS ONE 6:e16307. 2011
  5. ncbi The conserved KMN network constitutes the core microtubule-binding site of the kinetochore
    Iain M Cheeseman
    Ludwig Institute for Cancer Research, Department of Cellular and Molecular Medicine UCSD, CMM East, Room 3052, La Jolla, CA 92093, USA
    Cell 127:983-97. 2006
  6. pmc Aurora B controls kinetochore-microtubule attachments by inhibiting Ska complex-KMN network interaction
    Ying Wai Chan
    Growth and Development, Biozentrum, University of Basel, 4056 Basel, Switzerland
    J Cell Biol 196:563-71. 2012
  7. pmc Temporal changes in Hec1 phosphorylation control kinetochore-microtubule attachment stability during mitosis
    Keith F Deluca
    Department of Biochemistry and Molecular Biology, Colorado State University, Fort Collins, CO 80523, USA
    J Cell Sci 124:622-34. 2011
  8. pmc Microarray analysis identifies a death-from-cancer signature predicting therapy failure in patients with multiple types of cancer
    Gennadi V Glinsky
    Sidney Kimmel Cancer Center, San Diego, California 92121, USA
    J Clin Invest 115:1503-21. 2005
  9. pmc Expression analysis of mitotic spindle checkpoint genes in breast carcinoma: role of NDC80/HEC1 in early breast tumorigenicity, and a two-gene signature for aneuploidy
    Ivan Bieche
    INSERM U735, Institut Curie Hôpital René Huguenin, St Cloud, France
    Mol Cancer 10:23. 2011
  10. doi siRNA-mediated knockdown against CDCA1 and KNTC2, both frequently overexpressed in colorectal and gastric cancers, suppresses cell proliferation and induces apoptosis
    Naoyuki Kaneko
    Department of Molecular Pathology, Tohoku University School of Medicine, Sendai, Miyagi 980 8575, Japan
    Biochem Biophys Res Commun 390:1235-40. 2009

Research Grants

  1. Kinetochore-Microtubule Interactions
    JOHN RICHARD MCINTOSH; Fiscal Year: 2012
  2. SACCHAROMYCES CEREVISIAE MICROTUBULE CYTOSKELETON
    Georjana Barnes; Fiscal Year: 2013
  3. Cervical/Vaginal Mucus and Microbicides
    Thomas Hope; Fiscal Year: 2012
  4. Cervical/Vaginal Mucus and Microbicides
    Thomas Hope; Fiscal Year: 2013
  5. Role of the Ndc80 Loop Domain and Cdt1 in Kinetochore Microtubule Attachments
    Dileep Varma; Fiscal Year: 2013
  6. Formin mDia Functions in Mitosis
    Yinghui Mao; Fiscal Year: 2013
  7. Kinetochore Function in Vertebrate Cells
    Jennifer G DeLuca; Fiscal Year: 2013
  8. CELL AND TUMOR GENETICS OF MITOTIC ARREST CHECKPOINTS
    PETER KARL SORGER; Fiscal Year: 2010
  9. Architecture-function analysis of the kinetochore motor
    Ajit Joglekar; Fiscal Year: 2013
  10. Mechanisms of "End On" Microtubule Attachment by the Kinetochore
    P Todd Stukenberg; Fiscal Year: 2011

Detail Information

Publications192 found, 100 shown here

  1. ncbi Delayed nausea and vomiting continue to reduce patients' quality of life after highly and moderately emetogenic chemotherapy despite antiemetic treatment
    Brigitte Bloechl-Daum
    Department of Clinical Pharmacology, Medical University of Vienna, Vienna, Austria
    J Clin Oncol 24:4472-8. 2006
    ..the impact of acute (during the first 24 hours postchemotherapy) and delayed (days 2 through 5 postchemotherapy) CINV on patients' quality of life (QoL) after highly or moderately emetogenic chemotherapy (HEC and MEC, respectively).
  2. ncbi Cost-effectiveness analysis of aprepitant in the prevention of chemotherapy-induced nausea and vomiting in Belgium
    Lieven Annemans
    Ghent University, De Pintelaan 185, 9000, Gent, Belgium
    Support Care Cancer 16:905-15. 2008
    ..response (no emesis and no rescue therapy) compared to standard prevention, in patients receiving either highly (HEC) or moderately emetogenic chemotherapy (MEC; absolute reduction = 11 and 13%, respectively)...
  3. pmc Semi-solid gels function as physical barriers to human immunodeficiency virus transport in vitro
    Bonnie E Lai
    Department of Biomedical Engineering, Duke University, Durham, NC 27708, USA
    Antiviral Res 88:143-51. 2010
    ..Two gels used as placebos in microbicides clinical trials, hydroxyethyl cellulose (HEC) and methylcellulose (MC), were found to hinder HIV transport in vitro...
  4. pmc Abnormal kinetochore-generated pulling forces from expressing a N-terminally modified Hec1
    Marta Mattiuzzo
    Institute of Molecular Biology and Pathology, National Research Council of Italy, Rome, Italy
    PLoS ONE 6:e16307. 2011
    b>Highly Expressed in Cancer protein 1 (Hec1) is a constituent of the Ndc80 complex, a kinetochore component that has been shown to have a fundamental role in stable kinetochore-microtubule attachment, chromosome alignment and spindle ..
  5. ncbi The conserved KMN network constitutes the core microtubule-binding site of the kinetochore
    Iain M Cheeseman
    Ludwig Institute for Cancer Research, Department of Cellular and Molecular Medicine UCSD, CMM East, Room 3052, La Jolla, CA 92093, USA
    Cell 127:983-97. 2006
    ..Here, we examined the conserved KNL-1/Mis12 complex/Ndc80 complex (KMN) network, which is essential for kinetochore-microtubule interactions in vivo...
  6. pmc Aurora B controls kinetochore-microtubule attachments by inhibiting Ska complex-KMN network interaction
    Ying Wai Chan
    Growth and Development, Biozentrum, University of Basel, 4056 Basel, Switzerland
    J Cell Biol 196:563-71. 2012
    The KMN network (named according to the acronym for KNL1, Mis12, and Ndc80) and the more recently identified Ska complex (Ska1-3) have been shown to mediate kinetochore (KT)-microtubule (MT) attachments...
  7. pmc Temporal changes in Hec1 phosphorylation control kinetochore-microtubule attachment stability during mitosis
    Keith F Deluca
    Department of Biochemistry and Molecular Biology, Colorado State University, Fort Collins, CO 80523, USA
    J Cell Sci 124:622-34. 2011
    ..Here, we identify multiple serine residues within the N-terminus of the kinetochore protein Hec1 that are phosphorylated in an Aurora-B-kinase-dependent manner during mitosis...
  8. pmc Microarray analysis identifies a death-from-cancer signature predicting therapy failure in patients with multiple types of cancer
    Gennadi V Glinsky
    Sidney Kimmel Cancer Center, San Diego, California 92121, USA
    J Clin Invest 115:1503-21. 2005
    ....
  9. pmc Expression analysis of mitotic spindle checkpoint genes in breast carcinoma: role of NDC80/HEC1 in early breast tumorigenicity, and a two-gene signature for aneuploidy
    Ivan Bieche
    INSERM U735, Institut Curie Hôpital René Huguenin, St Cloud, France
    Mol Cancer 10:23. 2011
    ..Alterations of the mitotic spindle checkpoint are likely to contribute to these phenotypes, but little is known about somatic alterations of mitotic spindle checkpoint genes in breast cancer...
  10. doi siRNA-mediated knockdown against CDCA1 and KNTC2, both frequently overexpressed in colorectal and gastric cancers, suppresses cell proliferation and induces apoptosis
    Naoyuki Kaneko
    Department of Molecular Pathology, Tohoku University School of Medicine, Sendai, Miyagi 980 8575, Japan
    Biochem Biophys Res Commun 390:1235-40. 2009
    ..It is composed of two heterodimers, CDCA1-KNTC2 and SPC24-SPC25...
  11. pmc Mitotic control of kinetochore-associated dynein and spindle orientation by human Spindly
    Ying Wai Chan
    Department of Cell Biology, Max Planck Institute of Biochemistry, D 82152 Martinsried, Germany
    J Cell Biol 185:859-74. 2009
    ..Collectively, our data reveal hSpindly-mediated dynein functions and highlight a critical role of KT dynein in spindle orientation...
  12. pmc Inner centromere formation requires hMis14, a trident kinetochore protein that specifically recruits HP1 to human chromosomes
    Tomomi Kiyomitsu
    Core Research for Evolutional Science and Technology Research Program, Japan Science and Technology Corporation, Department of Gene Mechanisms, Graduate School of Biostudies, Kyoto University, Sakyo ku, Kyoto 606 8501, Japan
    J Cell Biol 188:791-807. 2010
    ..hMis14, which contains a tripartite-binding domain for HP1 and two other kinetochore proteins, hMis13 and blinkin, is a cornerstone for the assembly of the inner centromere and kinetochore...
  13. doi The mitotic checkpoint kinase NEK2A regulates kinetochore microtubule attachment stability
    J Du
    Hefei National Laboratory for Physical Sciences at Micro scale, University of Science and Technology of China, Hefei, China
    Oncogene 27:4107-14. 2008
    ..by NEK2A, which prevents aberrant kinetochore-microtubule connections in vivo, increases the affinity of the Ndc80 complex for microtubules in vitro...
  14. ncbi Phosphorylation of the mitotic regulator protein Hec1 by Nek2 kinase is essential for faithful chromosome segregation
    Yumay Chen
    Institute of Biotechnology, Department of Molecular Medicine, University of Texas Health Science Center at San Antonio, 15355 Lambda Drive, San Antonio, TX 78245 3207, USA
    J Biol Chem 277:49408-16. 2002
    b>Hec1 (highly expressed in cancer) plays essential roles in chromosome segregation by interacting through its coiled-coil domains with several proteins that modulate the G(2)/M phase...
  15. ncbi Kinetochore microtubule dynamics and attachment stability are regulated by Hec1
    Jennifer G DeLuca
    Department of Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
    Cell 127:969-82. 2006
    ..We report here that Ndc80/Hec1 functions in regulating kinetochore microtubule plus-end dynamics and attachment stability...
  16. ncbi NEK2A interacts with MAD1 and possibly functions as a novel integrator of the spindle checkpoint signaling
    Yang Lou
    School of Life Sciences, University of Science and Technology of China, Hefei 230027, China
    J Biol Chem 279:20049-57. 2004
    ..NEK2A is required for MAD2 but not MAD1, BUB1, and HEC1 to associate with kinetochores...
  17. pmc The Ndc80 kinetochore complex forms oligomeric arrays along microtubules
    Gregory M Alushin
    Biophysics Graduate Group, University of California, Berkeley, California 94720, USA
    Nature 467:805-10. 2010
    The Ndc80 complex is a key site of regulated kinetochore-microtubule attachment (a process required for cell division), but the molecular mechanism underlying its function remains unknown...
  18. pmc Orientation and structure of the Ndc80 complex on the microtubule lattice
    Elizabeth M Wilson-Kubalek
    Department of Cell Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
    J Cell Biol 182:1055-61. 2008
    The four-subunit Ndc80 complex, comprised of Ndc80/Nuf2 and Spc24/Spc25 dimers, directly connects kinetochores to spindle microtubules...
  19. ncbi Nuf2 and Hec1 are required for retention of the checkpoint proteins Mad1 and Mad2 to kinetochores
    Jennifer G DeLuca
    Department of Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
    Curr Biol 13:2103-9. 2003
    Members of the Ndc80/Nuf2 complex have been shown in several systems to be important in formation of stable kinetochore-microtubule attachments and chromosome alignment in mitosis...
  20. ncbi The Ndc80/HEC1 complex is a contact point for kinetochore-microtubule attachment
    Ronnie R Wei
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, 250 Longwood Avenue, Boston, Massachusetts 02115, USA
    Nat Struct Mol Biol 14:54-9. 2007
    ..Kinetochores are multicomponent assemblies that connect chromosomal centromeres to mitotic-spindle microtubules. The Ndc80 complex is an essential core element of kinetochores, conserved from yeast to humans...
  21. ncbi Role of Hec1 in spindle checkpoint signaling and kinetochore recruitment of Mad1/Mad2
    Silvia Martin-Lluesma
    Department of Cell Biology, Max Planck Institute of Biochemistry, Am Klopferspitz 18a, D 82152 Martinsried, Germany
    Science 297:2267-70. 2002
    ..We showed that the human protein Hec1 was required for the recruitment of Mps1 kinase and Mad1/Mad2 complexes to kinetochores...
  22. ncbi Expression of the kinetochore protein Hec1 during the cell cycle in normal and cancer cells and its regulation by the pRb pathway
    Chiara Ferretti
    Institute of Molecular Biology and Pathology, CNR, Rome, Italy
    Cell Cycle 9:4174-82. 2010
    b>Highly Expressed in Cancer protein 1 (Hec1) is a subunit of the Ndc80 complex, a constituent of the mitotic kinetochore...
  23. ncbi Polo-like kinase 1 creates the tension-sensing 3F3/2 phosphoepitope and modulates the association of spindle-checkpoint proteins at kinetochores
    Leena J Ahonen
    Molecular, Cell and Developmental Biology Research Program, Oklahoma Medical Research Foundation, Oklahoma City, OK 73104, USA
    Curr Biol 15:1078-89. 2005
    ..Determining the kinase that generates the 3F3/2 phosphoepitope at kinetochores should reveal an important component of this system that regulates mitotic progression...
  24. doi Implications for kinetochore-microtubule attachment from the structure of an engineered Ndc80 complex
    Claudio Ciferri
    Department of Experimental Oncology, European Institute of Oncology, Via Adamello 16, I 20139 Milan, Italy
    Cell 133:427-39. 2008
    ..The 180 kDa Ndc80 complex is a direct point of contact between kinetochores and microtubules...
  25. ncbi Hec1 sequentially recruits Zwint-1 and ZW10 to kinetochores for faithful chromosome segregation and spindle checkpoint control
    Y T Lin
    Department of Biological Chemistry, University of California, Irvine, CA 92697 4037, USA
    Oncogene 25:6901-14. 2006
    ..Previously, we discovered a novel coiled-coil protein, highly expressed in cancer 1 (Hec1), which is indispensable for this process. However, the precise underlying mechanism remains unclear...
  26. pmc A conserved protein network controls assembly of the outer kinetochore and its ability to sustain tension
    Iain M Cheeseman
    Ludwig Institute for Cancer Research, La Jolla, California 92093, USA
    Genes Dev 18:2255-68. 2004
    ..Thus, this protein network is a conserved constituent of the outer kinetochore, and the functions defined by our analysis in C. elegans are likely to be widely relevant...
  27. ncbi Timing and checkpoints in the regulation of mitotic progression
    Patrick Meraldi
    Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Dev Cell 7:45-60. 2004
    ..We propose that cytosolic Mad2-BubR1 is essential to restrain anaphase onset early in mitosis when kinetochores are still assembling...
  28. ncbi A conserved Mis12 centromere complex is linked to heterochromatic HP1 and outer kinetochore protein Zwint-1
    Chikashi Obuse
    Department of Gene Mechanisms, Graduate School of Biostudies, Kyoto University, Yoshida Honmachi, Sakyo ku, Kyoto 606 8501, Japan
    Nat Cell Biol 6:1135-41. 2004
    ..Nine polypeptides bind to human hMis12; two of these, HEC1 and Zwint-1, are authentic kinetochore proteins...
  29. pmc Timely anaphase onset requires a novel spindle and kinetochore complex comprising Ska1 and Ska2
    Anja Hanisch
    Department of Cell Biology, Max Planck Institute for Biochemistry, Martinsried, Germany
    EMBO J 25:5504-15. 2006
    ..These data suggest that the Ska1/2 complex plays a critical role in the maintenance of the metaphase plate and/or spindle checkpoint silencing...
  30. pmc The NDC80 complex proteins Nuf2 and Hec1 make distinct contributions to kinetochore-microtubule attachment in mitosis
    Lynsie J R Sundin
    Department of Biochemistry and Molecular Biology, Colorado State University, Fort Collins, CO 80523, USA
    Mol Biol Cell 22:759-68. 2011
    ..Central to generating these attachments is the NDC80 complex, made of the four proteins Spc24, Spc25, Nuf2, and Hec1/Ndc80...
  31. ncbi Architecture of the human ndc80-hec1 complex, a critical constituent of the outer kinetochore
    Claudio Ciferri
    Department of Experimental Oncology, European Institute of Oncology, Via Ripamonti 435, 20141 Milan, Italy
    J Biol Chem 280:29088-95. 2005
    The Ndc80 complex is a constituent of the outer plate of the kinetochore and plays a critical role in establishing the stable kinetochore-microtubule interactions required for chromosome segregation in mitosis...
  32. ncbi The retinoblastoma protein associates with the protein phosphatase type 1 catalytic subunit
    T Durfee
    Center for Molecular Medicine Institute of Biotechnology, University of Texas Health Science Center, San Antonio 78245
    Genes Dev 7:555-69. 1993
    ..Cell cycle synchrony experiments revealed that this association occurs from mitosis to early G1. The implications of these findings on the regulation of both proteins are discussed...
  33. pmc Phosphorylation of the Ndc80 complex protein, HEC1, by Nek2 kinase modulates chromosome alignment and signaling of the spindle assembly checkpoint
    Randy Wei
    Department of Biological Chemistry, School of Medicine, University of California, Irvine, Irvine, CA 92697, USA
    Mol Biol Cell 22:3584-94. 2011
    The spindle assemble checkpoint (SAC) is critical for accurate chromosome segregation. Hec1 contributes to chromosome segregation in part by mediating SAC signaling and chromosome alignment...
  34. pmc ZW10 links mitotic checkpoint signaling to the structural kinetochore
    Geert J P L Kops
    Ludwig Institute for Cancer Research and Department of Cellular and Molecular Medicine, University of California at San Diego, La Jolla, CA 92093, USA
    J Cell Biol 169:49-60. 2005
    ..ZW10 partner, Zwint-1, is part of a separate complex of structural kinetochore components including Mis12 and Ndc80-Hec1. Zwint-1 is critical for recruiting ZW10 to unattached kinetochores...
  35. pmc Hec1 contributes to mitotic centrosomal microtubule growth for proper spindle assembly through interaction with Hice1
    Guikai Wu
    Department of Biological Chemistry, School of Medicine, University of California, Irvine, Irvine, CA 92697, USA
    Mol Biol Cell 20:4686-95. 2009
    Previous studies have stipulated Hec1 as a conserved kinetochore component critical for mitotic control in part by directly binding to kinetochore fibers of the mitotic spindle and by recruiting spindle assembly checkpoint proteins Mad1 ..
  36. ncbi Identification of two novel components of the human NDC80 kinetochore complex
    Rajnish Bharadwaj
    Department of Pharmacology, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75390, USA
    J Biol Chem 279:13076-85. 2004
    ..Components of the conserved NDC80 complex are required for chromosome congression, and their disruption results in mitotic arrest accompanied by ..
  37. pmc HEC, a novel nuclear protein rich in leucine heptad repeats specifically involved in mitosis
    Y Chen
    Department of Molecular Medicine, Institute of Biotechnology, University of Texas Health Science Center at San Antonio, 78245, USA
    Mol Cell Biol 17:6049-56. 1997
    The protein encoded by the human gene HEC (highly expressed in cancer) contains 642 amino acids and a long series of leucine heptad repeats at its C-terminal region...
  38. ncbi An apocrine membrane antigen with polarized distribution and hormonally regulated expression in human endometrial and mammary carcinoma cell lines
    L M Forsman
    Department of Pathology, University of Helsinki, Finland
    Acta Pathol Microbiol Immunol Scand A 95:315-23. 1987
    ..of an apocrine epithelial differentiation antigen (AEA) were studied in one endometrial adenocarcinoma cell line (HEC-1-B) and two mammary carcinoma cell lines (MCF-7 and T47-D), using an antiserum raised against glycoproteins which ..
  39. ncbi Gene amplification and expression of the steroid receptor coactivator SRC3 (AIB1) in sporadic breast and endometrial carcinomas
    M Glaeser
    Department of Gynecology and Obstetrics, Heinrich-Heine-University, Duesseldorf, Germany
    Horm Metab Res 33:121-6. 2001
    ..breast carcinomas, 30 endometrial carcinomas and different cell lines (MCF7, HeLa, Ishikawa, T47D, BT-20, SK-BR-3, HEC-1a, RL 95-2, OVCAR3 and A-431)...
  40. ncbi Quantitative studies on the adsorption of proteins to the bare silica wall in capillary electrophoresis. II. Effects of adsorbed, neutral polymers on quenching the interaction
    B Verzola
    University of Verona, Department of Agricultural and Industrial Biotechnology, Italy
    J Chromatogr A 874:293-303. 2000
    ..of proteins with the silica wall: hydroxypropylmethylcellulose (HPMC, Mr = 1000000), hydroxyethylcellulose (HEC, Mr = 27000), poly(vinyl alcohol) (PVA, Mr = 49000) and short-chain poly(dimethylacrylamide) [poly(DMA)] (average ..
  41. pmc Molecular analysis of core kinetochore composition and assembly in Drosophila melanogaster
    Marcin R Przewloka
    Cancer Research UK, Cell Cycle Genetics Research Group, Department of Genetics, University of Cambridge, Cambridge, United Kingdom
    PLoS ONE 2:e478. 2007
    ..Although Drosophila is a classical model organism for studies of chromosome segregation, little is known about the organization of its kinetochores...
  42. doi Towards elucidation of the drug release mechanism from compressed hydrophilic matrices made of cellulose ethers. I. Pulse-field-gradient spin-echo NMR study of sodium salicylate diffusivity in swollen hydrogels with respect to polymer matrix physical stru
    Carmen Ferrero
    Department of Pharmaceutics and Biopharmaceutics, School of Pharmaceutical Sciences, University of Geneva, University of Lausanne, Quai Ernest Ansermet 30, 1211 Geneva 4, Switzerland
    J Control Release 128:71-9. 2008
    ..and, incidentally, that of water, in hydrogels made of hydroxypropyl methylcellulose (HPMC), hydroxyethylcellulose (HEC) and hydroxypropylcellulose (HPC) of varying polymer weight fraction and molecular weight in D2O...
  43. ncbi Capillary electrophoresis of DNA in uncross-linked polymer solutions
    A E Barron
    Department of Chemical Engineering, University of California, Berkeley 94720
    J Chromatogr A 652:3-16. 1993
    We have used dilute and semi-dilute uncross-linked hydroxyethyl cellulose (HEC) solutions as separation matrices for capillary electrophoresis of DNA restriction fragments...
  44. ncbi [Health care ethics committees in German university clinics. A survey of all medical directors and directors of nursing]
    J Vollmann
    Friedrich Alexander Universitat Erlangen Nurnberg, Erlangen
    Dtsch Med Wochenschr 129:1237-42. 2004
    Health Care Ethics Committees (HEC) are new ways of implementing clinical ethics in hospitals and nursing homes. In the US all health care institutions must provide some structure to handle ethical conflicts in everyday patients' care...
  45. ncbi Quantitation of protein binding to the capillary wall in acidic, isoelectric buffers and means for minimizing the phenomenon
    E Olivier
    Department of Agricultural and Industrial Biotechnology, University of Verona, Italy
    J Chromatogr A 894:273-80. 2000
    ..7 in 7 M urea). Three potential quenchers of such interactions have been tested: hydroxyethylcellulose (HEC; number average molecular mass, Mr 27,000), TEPA (tetraethylenepentamine) and a novel, quatemarized piperazine [N(..
  46. pmc Kinetochore attachments require an interaction between unstructured tails on microtubules and Ndc80(Hec1)
    Stephanie A Miller
    Department of Biochemistry and Molecular Genetics, University of Virginia School of Medicine, Charlottesville, VA 22908, USA
    Curr Biol 18:1785-91. 2008
    ..Kinetochores are unable to bind microtubules after any of the four subunits of the Ndc80 complex are knocked down [2, 4]; however, because the Ndc80 complex has important structural roles [1-3], it is ..
  47. pmc The highly conserved Ndc80 complex is required for kinetochore assembly, chromosome congression, and spindle checkpoint activity
    Mark L McCleland
    Department of Biochemistry and Molecular Genetics, University of Virginia Medical School, Charlottesville, Virginia 22908, USA
    Genes Dev 17:101-14. 2003
    We show that the Xenopus homologs of Ndc80/Tid3/HEC1 (xNdc80) and Nuf2/MPP1/Him-10 (xNuf2) proteins physically interact in a 190-kD complex that associates with the outer kinetochore from prometaphase through anaphase...
  48. doi Efficacy and safety of casopitant mesylate, a neurokinin 1 (NK1)-receptor antagonist, in prevention of chemotherapy-induced nausea and vomiting in patients receiving cisplatin-based highly emetogenic chemotherapy: a randomised, double-blind, placebo-contr
    Steven M Grunberg
    University of Vermont, Burlington, VT 05405, USA
    Lancet Oncol 10:549-58. 2009
    ..and vomiting (CINV) remains a clinical management problem after treatment with highly emetogenic chemotherapy (HEC)...
  49. ncbi Ethicians, ethicists and the goals of clinical ethics consultation
    Frederick Adolf Paola
    Health Professions Division, Nova Southeastern University, 2655 Northbrooke Drive, Naples, FL 34119, USA
    Intern Emerg Med 1:5-14. 2006
    ..In pursuing these ends, healthcare ethics consultation (HEC) and CEC services confront three broad categories of questions: (1) questions of professional duty; (2) questions ..
  50. doi Low incidence of enterocolitis and colonic mucosal inflammation in Norwegian patients with Hirschsprung's disease
    Yasser Rehman
    Department of Surgery, Faculty of Medicine, University of Oslo, Oslo, Norway
    Pediatr Surg Int 25:133-8. 2009
    ..Further, the etiology of Hirschsprung-associated enterocolitis (HEC) is unclear...
  51. ncbi Transbuccal permeation, anti-inflammatory activity and clinical efficacy of piroxicam formulated in different gels
    M A Attia
    Department of Pharmaceutics, Faculty of Pharmacy, Assiut University, Assiut, Egypt
    Int J Pharm 276:11-28. 2004
    ..5%) >or= sodium alginate (Na alg., 7%) > methylcellulose (MC, 3%) > hydroxyethylcellulose (HEC, 1.5%) > carbopol 934 (Carb...
  52. pmc The Ndc80 complex uses a tripartite attachment point to couple microtubule depolymerization to chromosome movement
    John G Tooley
    Department of Biochemistry and Molecular Genetics, University of Virginia Medical Center, Charlottesville, VA 22908, USA
    Mol Biol Cell 22:1217-26. 2011
    In kinetochores, the Ndc80 complex couples the energy in a depolymerizing microtubule to perform the work of moving chromosomes...
  53. pmc Invasion of human mucosal epithelial cells by Neisseria gonorrhoeae upregulates expression of intercellular adhesion molecule 1 (ICAM-1)
    G A Jarvis
    Department of Laboratory Medicine, University of California San Francisco, San Francisco, California
    Infect Immun 67:1149-56. 1999
    ..invasive strain FA1090 and the poorly invasive strain MS11 were incubated with human endometrial adenocarcinoma (HEC-1-B) or human cervical carcinoma (ME-180) epithelial cells, after which ICAM-1 expression was measured by flow ..
  54. pmc The Ndc80 kinetochore complex forms load-bearing attachments to dynamic microtubule tips via biased diffusion
    Andrew F Powers
    Department of Physiology and Biophysics, University of Washington, Seattle, 98195, USA
    Cell 136:865-75. 2009
    ..Genetic, biochemical, and structural studies implicate the Ndc80 complex as a direct point of contact between kinetochores and microtubules, but these approaches provide only a ..
  55. pmc Multiple mechanisms of chromosome movement in vertebrate cells mediated through the Ndc80 complex and dynein/dynactin
    Valeriya V Vorozhko
    Molecular, Cell and Developmental Biology Research Program, Oklahoma Medical Research Foundation, 825 NE 13th St, MS 48, Oklahoma City, OK 73104, USA
    Chromosoma 117:169-79. 2008
    ..chromosomes, we found microtubule nucleation/binding depended additively on both dynein/dynactin and on the Ndc80/Hec1 complex...
  56. ncbi Effect of acute hyperglycemia and/or hyperinsulinemia on polymorphonuclear functions in healthy subjects
    Vladimira Fejfarova
    Institute for Clinical and Experimental Medicine, Prague 140 21, Czech Republic
    Metabolism 55:811-8. 2006
    ..by three 4-hour-long clamp studies-hyperglycemic hyperinsulinemic clamp (HHC), hyperinsulinemic euglycemic clamp (HEC), and isolated hyperglycemic clamp with insulin secretion blockade (HGC)...
  57. doi A survey of Louisiana hospital ethics committees
    Thomas P Gonsoulin
    Tulane Department of Otolaryngology, Head and Neck Surgery, Tulane Health Sciences Center, New Orleans, LA, USA
    Laryngoscope 119:330-40. 2009
    ..To obtain a picture of hospital ethics committees (HECs) in the State of Louisiana. Compare results to other published studies. Gauge interest and participation in HECs by otolaryngologists...
  58. ncbi Correlation of in vitro chemopreventive efficacy data from the human epidermal cell assay with animal efficacy data and clinical trial plasma levels
    Eugene Elmore
    Department of Radiation Oncology, University of California Irvine, Irvine, CA 92697, USA
    J Cell Biochem 95:571-88. 2005
    The human epidermal cell (HEC) assay, which uses carcinogen exposed normal skin keratinocytes to screen for cancer prevention efficacy, was used to screen possible preventive agents...
  59. ncbi HER-2 amplification and topoisomerase IIalpha gene aberrations as predictive markers in node-positive breast cancer patients randomly treated either with an anthracycline-based therapy or with cyclophosphamide, methotrexate, and 5-fluorouracil
    Angelo Di Leo
    Translational Research Unit, Jules Bordet Institute Brussels, 1000, Belgium
    Clin Cancer Res 8:1107-16. 2002
    ..The purpose of this study is to evaluate HER-2 and topoisomerase IIalpha (topo IIalpha) as candidates for predicting the activity of anthracyclines in the adjuvant treatment of breast cancer patients...
  60. ncbi [Effects and mechanisms of 5-aza-2'-deoxycytidine on endometrial cancer cell.]
    Yu Quan Zhang
    Department of Obstetrics and Gynecology, Affiliated Hospital of Nantong University, Nantong 226000, China Email
    Zhonghua Fu Chan Ke Za Zhi 44:861-4. 2009
    ..To investigate the effects and mechanisms of 5-aza-2'-deoxycytidine (5-Aza-CdR) on endometrial cancer cell...
  61. pmc Linking binge alcohol-induced neurodamage to brain edema and potential aquaporin-4 upregulation: evidence in rat organotypic brain slice cultures and in vivo
    Kumar Sripathirathan
    CoMentis Inc, Oklahoma City, Oklahoma, USA
    J Neurotrauma 26:261-73. 2009
    Brain edema and derived oxidative stress potentially are critical events in the hippocampal-entorhinal cortical (HEC) neurodegeneration caused by binge alcohol (ethanol) intoxication and withdrawal in adult rats...
  62. pmc Mechanisms of eosinophil adherence to cultured vascular endothelial cells. Eosinophils bind to the cytokine-induced ligand vascular cell adhesion molecule-1 via the very late activation antigen-4 integrin receptor
    A Dobrina
    Institute of General Pathology, University of Trieste, Italy
    J Clin Invest 88:20-6. 1991
    ..in the adherence of normal peripheral blood eosinophils to cultured human umbilical vein endothelial cells (HEC) under three conditions: (a) adherence in the absence of treatment of HEC or eosinophils with activating agents (..
  63. doi Primary chemoprevention of endometrial hyperplasia with the peroxisome proliferator-activated receptor gamma agonist rosiglitazone in the PTEN heterozygote murine model
    W Wu
    Department of Gynecologic Oncology, The University of Texas M D Anderson Cancer Center, Houston, Texas 77030, USA
    Int J Gynecol Cancer 18:329-38. 2008
    ..b>Hec-1-A (PTEN wild type) and Ishikawa (PTEN null) cells were treated with rosiglitazone...
  64. ncbi Design, characterisation and preliminary clinical evaluation of a novel mucoadhesive topical formulation containing tetracycline for the treatment of periodontal disease
    D S Jones
    School of Pharmacy, The Queen s University of Belfast, Medical Biology Centre, 97, Lisburn Road, BT9 7BL, Northern Ireland, Belfast, UK
    J Control Release 67:357-68. 2000
    ..and preliminary clinical evaluation of mucoadhesive, semi-solid formulations containing hydroxyethylcellulose (HEC, 1-5%, w/w), polyvinylpyrrolidine (PVP, 2 or 3%, w/w), polycarbophil (PC, 1 or 3%, w/w) and tetracycline (5%, w/w, ..
  65. ncbi The impact of urea on viscosity of hydroxethyl cellulose and observed mobility of deoxyribonucleic acids
    O Otim
    Beckman Institute 139 74, Division of Biology, California Institute of Technology, Pasadena 91125, USA
    Biopolymers 58:329-34. 2001
    The influence of urea on the viscosity of hydroxyethyl cellulose (HEC), and the state and separation of double-stranded DNA, was studied by viscometry, fluorometry, and capillary electrophoresis...
  66. ncbi A pilot study on hyperinsulinaemic euglycaemic clamp based insulin sensitivity in young adult Indian males with low body mass index
    Gajalakshmi Ramakrishnan
    Department of Physiology, St John s Medical College and Research Institute Bangalore, India
    Indian J Med Res 129:409-17. 2009
    ..This study thus used the hyperinsulinaemic euglycaemic clamp (HEC) technique to compare IS in young normal weight (NW) and low BMI (LBMI) Indian males...
  67. ncbi Liposomal drugs dispersed in hydrogels. Effect of liposome, drug and gel properties on drug release kinetics
    Spyridon Mourtas
    Laboratory of Pharmaceutical Technology, Department of Pharmacy, School of Health Sciences, University of Patras, 26510 Rio, Greece
    Colloids Surf B Biointerfaces 55:212-21. 2007
    ..5 or 2% (w/w)], hydroxylethyl-cellulose (HEC) [4% (w/w)], or a mixture of the two), and release of calcein or GRF was followed by fluorescence or photometric ..
  68. ncbi Prevalence of overweight and obesity among children and adolescents of affluent schools in Karachi
    Sina Aziz
    Sarwar Zuberi Liver Centre, Medical Unit 5, Civil Hospital Karachi, Dow University of Health Sciences, Karachi, Pakistan
    J Pak Med Assoc 59:35-8. 2009
    ..To estimate the prevalence of overweight and obesity among children and adolescents of affluent schools of Karachi...
  69. ncbi [Foundation of a Health Care Ethics Committee (HEC) in a nonconfessional hospital. Initial experiences and prospects of the future]
    Norbert Scheffold
    Medizinische Klinik I, SLK Kliniken Heilbronn GmbH, Klinikum am Gesundbrunnen, Heilbronn
    Med Klin (Munich) 101:584-9. 2006
    ..In this study, the authors report of the implementation of an HEC at the SLK Hospital Heilbronn, Germany, and discuss the further development within the first 2 years.
  70. doi Chloroacetaldehyde-induced mutagenesis in Escherichia coli: the role of AlkB protein in repair of 3,N(4)-ethenocytosine and 3,N(4)-alpha-hydroxyethanocytosine
    Agnieszka M Maciejewska
    Institute of Biochemistry and Biophysics, Polish Academy of Sciences, 02 106 Warsaw, 5A Pawińskiego Str, Poland
    Mutat Res 684:24-34. 2010
    ..Formation of varepsilonC proceeds via a relatively stable intermediate, 3,N(4)-alpha-hydroxyethanocytosine (HEC), which allowed to compare repair of both adducts...
  71. pmc A bipartite signal mediates the transfer of type IV secretion substrates of Bartonella henselae into human cells
    Ralf Schulein
    Division of Molecular Microbiology, Biozentrum, University of Basel, Klingelbergstrasse 70, 4056 Basel, Switzerland
    Proc Natl Acad Sci U S A 102:856-61. 2005
    ..VirB of the vascular tumor-inducing pathogen Bartonella henselae causes subversion of human endothelial cell (HEC) function...
  72. ncbi Novel chondroitin sulfate-modified ligands for L-selectin on lymph node high endothelial venules
    C J Derry
    Division of Cellular Immunology, National Institute for Medical Research, London, GB
    Eur J Immunol 29:419-30. 1999
    ..High endothelial cells (HEC) cultured from rat lymph node HEV were shown to express ligands for L-selectin...
  73. doi Estrogen formation in endometrial and cervix cancer cell lines: involvement of aromatase, steroid sulfatase and 17beta-hydroxysteroid dehydrogenases (types 1, 5, 7 and 12)
    Michelle Audrey Fournier
    Oncology and Molecular Endocrinology Laboratory, Medicinal Chemistry Division, CHUQ CHUL Research Center and Université Laval, Quebec, Quebec G1V 4G2, Canada
    Mol Cell Endocrinol 301:142-5. 2009
    ..17beta-HSDs) in the production of estrogens was determined in four cell lines of endometrial cancer (Ishikawa, HEC-1A, HEC-1B and RL-95) and one cell line of cervix cancer (Hela) in culture...
  74. doi Ethical reasoning about patient eligibility in allogeneic BMT based on psychosocial criteria
    L W Foster
    School of Social Work, Cleveland State University, Cleveland, OH 44115 2214, USA
    Bone Marrow Transplant 44:607-12. 2009
    ..A self-administered questionnaire was sent to 62 HEC chairpersons at hospitals with an accredited BMT program; the response rate was 37%...
  75. ncbi Cytokine-induced beta-galactoside alpha-2,6-sialyltransferase in human endothelial cells mediates alpha 2,6-sialylation of adhesion molecules and CD22 ligands
    K Hanasaki
    Division of Cellular and Molecular Medicine, University of California, San Diego, La Jolla 92093
    J Biol Chem 269:10637-43. 1994
    ..Here we show that treatment of cultured human endothelial cells (HEC) with TNF-alpha, interleukin-1, or lipopolysaccharide causes increased expression of the enzyme beta-galactoside ..
  76. ncbi Phase III trial comparing two dose levels of epirubicin combined with cyclophosphamide with cyclophosphamide, methotrexate, and fluorouracil in node-positive breast cancer
    M J Piccart
    Jules Bordet Institute, Belgian and Luxembourg Cooperative Centers, and Pharmacia Upjohn, Brussels, Belgium
    J Clin Oncol 19:3103-10. 2001
    To compare a full-dose epirubicin-cyclophosphamide (HEC) regimen with classical cyclophosphamide, methotrexate, and fluorouracil (CMF) therapy and with a moderate-dose epirubicin-cyclophosphamide regimen (EC) in the adjuvant therapy of ..
  77. doi First-line trastuzumab plus epirubicin and cyclophosphamide therapy in patients with human epidermal growth factor receptor 2-positive metastatic breast cancer: cardiac safety and efficacy data from the Herceptin, Cyclophosphamide, and Epirubicin (HERCULE
    Michael Untch
    Helios Klinikum Berlin Buch, Frauenheilkunde mit Geburtshilfe, Berlin, Germany
    J Clin Oncol 28:1473-80. 2010
    ..The Herceptin, Cyclophosphamide, and Epirubicin (HERCULES) trial evaluated trastuzumab plus cyclophosphamide and the less cardiotoxic anthracycline epirubicin...
  78. ncbi In vitro sensitivity of human endometrial cancer cell lines to paclitaxel or irinotecan (CPT-11) in combination with other aniticancer drugs
    H P Hiramatsu
    Department of Obstetrics and Gynecology, Seto Hospital, Tokorozawa, Saitama, Japan
    Anticancer Drugs 11:573-8. 2000
    ..of patients with endometrial cancer, were examined using five human endometrial cancer cell lines (Ishikawa, HEC-1A, HEC-50B, HEC-59 and HEC-108)...
  79. ncbi Incidence of chemotherapy-induced nausea and emesis after modern antiemetics
    Steven M Grunberg
    Department of Medicine, University of Vermont, Burlington, Vermont 05401, USA
    Cancer 100:2261-8. 2004
    ..of acute and delayed chemotherapy-induced nausea and emesis (vomiting) (CINV) among patients receiving highly (HEC) or moderately (MEC) emetogenic chemotherapy...
  80. ncbi Cytocompatible gel formation of chitosan-glycerol phosphate solutions supplemented with hydroxyl ethyl cellulose is due to the presence of glyoxal
    C D Hoemann
    Department of Chemical Engineering, Ecole Polytechnique, PO Box 6079, Station Centre Ville, Montreal, Quebec, Canada H3C 3A7
    J Biomed Mater Res A 83:521-9. 2007
    ..developed an adhesive in situ-gelling cell carrier by suspending cells in a solution of hydroxyethyl cellulose (HEC), which was then mixed with chitosan-glycerol phosphate to form a chitosan-GP/HEC gel...
  81. doi Viscosification in polymer-surfactant mixtures at low temperatures
    Neda Beheshti
    Department of Chemistry, University of Oslo, P O Box 1033, Blindern, N 0315 Oslo, Norway
    J Phys Chem B 114:6273-80. 2010
    Interactions between the anionic surfactant sodium dodecyl sulfate (SDS) and hydroxyethylcellulose (HEC) or its hydrophobically modified analogue (HM-HEC) have been studied over an extended temperature region with the aid of turbidimetry,..
  82. pmc Dissociation of the Nuf2-Ndc80 complex releases centromeres from the spindle-pole body during meiotic prophase in fission yeast
    Haruhiko Asakawa
    Cell Biology Group and CREST Research Project, Kansai Advanced Research Center, National Institute of Information and Communications Technology, Kobe 651 2492, Japan
    Mol Biol Cell 16:2325-38. 2005
    ..Here we examined the behavior of centromere proteins in living meiotic cells of S. pombe. We show that the Nuf2-Ndc80 complex proteins (Nuf2, Ndc80, Spc24, and Spc25) disappear from the centromere in meiotic prophase when the ..
  83. ncbi Ocular poloxamer-based ciprofloxacin hydrochloride in situ forming gels
    Mai Mansour
    Department of Pharmaceutics, Faculty of Pharmacy, Ain Shams University, Cairo, Egypt
    Drug Dev Ind Pharm 34:744-52. 2008
    ..Mucoadhesives such as hydroxypropylmethyl cellulose (HPMC) or hydroxyethyl cellulose (HEC) were added to the formulations to enhance the gel bioadhesion properties...
  84. doi Predictors of adherent use of diaphragms and microbicide gel in a four-arm, randomized pilot study among female sex workers in Madagascar
    Abigail Norris Turner
    Department of Epidemiology, School of Public Health, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599 7435, USA
    Sex Transm Dis 36:249-57. 2009
    ..Participants' protocol adherence may influence assessments of the effectiveness of new female-controlled methods for sexually transmitted infection prevention...
  85. doi Vaginal microbicide and diaphragm use for sexually transmitted infection prevention: a randomized acceptability and feasibility study among high-risk women in Madagascar
    Frieda M Behets
    Department of Epidemiology, School of Public Health, University of North Carolina, Chapel Hill, North Carolina 27599 7435, USA
    Sex Transm Dis 35:818-26. 2008
    ....
  86. pmc Role of FOXO1A in the regulation of insulin-like growth factor-binding protein-1 in human endometrial cells: interaction with progesterone receptor
    J J Kim
    Division of Reproductive Biology Research, Department of Obstetrics and Gynecology, Northwestern University, Chicago, Illinois 60611, USA
    Biol Reprod 73:833-9. 2005
    ..receptor A (PGRA), and progesterone receptor B (PGRB) proteins, whereas the endometrial adenocarcinoma cell line, HEC-1B cells, expressed only FOXO1A and no detectable PGR proteins...
  87. ncbi Inhibition of growth of experimental human endometrial cancer by an antagonist of growth hormone-releasing hormone
    Jorg B Engel
    Veterans Affairs Medical Center, 1601 Perdido Street, New Orleans, Louisiana 70112 1262, USA
    J Clin Endocrinol Metab 90:3614-21. 2005
    ..we investigated in vivo and in vitro the effects of the GHRH antagonist MZ-J-7-118 and its mechanism of action in HEC-1A human endometrial cancer...
  88. pmc Lymphotoxin plays a crucial role in the development and function of nasal-associated lymphoid tissue through regulation of chemokines and peripheral node addressin
    Xiaoyan Ying
    Yale University School of Medicine, Department of Epidemiology and Public Health, 60 College St, P O Box 208034, New Haven, CT 06520 8034, USA
    Am J Pathol 166:135-46. 2005
    ..High endothelial venules (HEVs) developed that expressed GlyCAM-1, HEC-6ST [an enzyme crucial for expression of luminal peripheral node addressin (PNAd)], and PNAd itself...
  89. pmc Vaginal microbicides: detecting toxicities in vivo that paradoxically increase pathogen transmission
    Richard A Cone
    Mucosal Protection Laboratory, Dept of Biophysics, Johns Hopkins University, Baltimore, MD 21218, USA
    BMC Infect Dis 6:90. 2006
    ....
  90. ncbi Development of a model for inducing transient insulin resistance in the mare: preliminary implications regarding the estrous cycle
    D R Sessions
    Department of Veterinary Science, Maxwell Gluck Equine Research Center, University of Kentucky, Lexington 40546 0099, USA
    J Anim Sci 82:2321-8. 2004
    ..In Exp. 1, a hyperinsulinemic-euglycemic clamp (HEC) procedure was performed on seven mares to determine insulin sensitivity before and immediately following infusion ..
  91. ncbi A molecular phylogeny of the hawkmoth genus Hyles (Lepidoptera: Sphingidae, Macroglossinae)
    Anna K Hundsdoerfer
    Institut für Pharmazie und molekulare Biotechnologie, Abt Biologie, Im Neuenheimer Feld 364, D 69120 Heidelberg, Germany
    Mol Phylogenet Evol 35:442-58. 2005
    ..We propose that the radiation of the Hyles euphorbiae-complex s. str. (HEC) occurred as recently as the Pliocene/Pleistocene boundary and that its roots can still be reconstructed in Asia...
  92. pmc Protein disulfide isomerase, a component of the estrogen receptor complex, is associated with Chlamydia trachomatis serovar E attached to human endometrial epithelial cells
    C H Davis
    Department of Microbiology and Immunology, School of Medicine, University of North Carolina Chapel Hill, Chapel Hill, North Carolina, USA
    Infect Immun 70:3413-8. 2002
    ..apical membrane protein receptor(s) attached to EB at 4 degrees C was stripped off the surface of polarized HEC-1B cells and immunoprecipitated with polyclonal anti-EB antibodies, an approximately 55-kDa protein was ..
  93. ncbi Viscoelastic properties of bioadhesive, chlorhexidine-containing semi-solids for topical application to the oropharynx
    D S Jones
    The Pharmaceutical Sciences Group, School of Pharmacy, The Queen s University of Belfast, Medical Biology Centre, Northern Ireland, United Kingdom
    Pharm Res 15:1131-6. 1998
    ..This study examined the viscoelastic properties of bioadhesive, chlorhexidine-containing semi-solid formulations, designed for topical application to the oropharynx...
  94. ncbi Effect of p53 gene transfection on vascular endothelial growth factor expression in endometrial cancer cells
    T Fujisawa
    Department of Clinical Cytology, Graduate School of Medical Sciences, Kitasato University, Sagamihara, Kanagawa, Japan
    Exp Mol Pathol 74:276-81. 2003
    ..and the effect of p53 gene transfection on VEGF expression in the p53-mutated endometrial carcinoma cell line, HEC-50B. Immunoblotting for detecting VEGF, p53, and beta-actin was performed...
  95. pmc Phorbol ester causes down-regulation of CD11/CD18-independent neutrophil adherence to endothelium
    A Dobrina
    Institute of General Pathology, University of Trieste, Italy
    Immunology 69:429-34. 1990
    ..tumour necrosis factor (TNF)- or lipopolysaccharide (LPS)-pretreated human umbilical vein endothelial cells (HEC) by CD11/CD18-dependent and independent mechanisms...
  96. ncbi [Effect of signal transduction inhibitors on human endometrial carcinoma cells with differential PTEN gene expression]
    Lan Xiao
    Department of Gynecology, Third Affiliated Hospital, Sun Yat Sen University, Guangzhou 510630, China
    Zhonghua Fu Chan Ke Za Zhi 44:681-5. 2009
    ..To investigate the apoptotic and proliferation effects of signal transduction inhibitors on human endometrial carcinoma cells with different PTEN gene expression...
  97. doi Triptolide induces apoptosis in endometrial cancer via a p53‑independent mitochondrial pathway
    Xiao Fei Wang
    Changzhou Maternal and Child Health Care Hospital of Nanjing Medical University, Changzhou 213003, P R China
    Mol Med Rep 9:39-44. 2014
    ..In the present study, we investigated the antitumor effect of TP in human endometrial cancer cells (HEC-1B) and elucidated its possible underlying mechanisms...
  98. doi Association of depressive symptoms with recurrent falls: a cross-sectional elderly population based study and a systematic review
    C Launay
    Department of Neuroscience, Division of Geriatric Medicine, Angers University Hospital, Angers, France
    J Nutr Health Aging 17:152-7. 2013
    ..The association between abnormal 4-item GDS score and recurrent falls has not yet been examined. In addition, while depressive symptoms-related gait instability is well known, the association with recurrent falls has been few studied...
  99. doi Regulatory mechanisms of kinetochore-microtubule interaction in mitosis
    Kozo Tanaka
    Department of Molecular Oncology, Institute of Development, Aging and Cancer, Tohoku University, 4 1 Seiryo machi, Aoba ku, Sendai, 980 8575, Miyagi, Japan
    Cell Mol Life Sci 70:559-79. 2013
    ..Emerging evidence suggests that the KNL1-Mis12-Ndc80 (KMN) network plays a central role in connecting kinetochores to microtubules, which is under fine regulation by a ..
  100. ncbi An integrated inundation model for highly developed urban areas
    A S Chen
    National Science and Technology Center for Disaster Reduction, Taipei, Chinese Taiwan 106
    Water Sci Technol 51:221-9. 2005
    ..The model integrates the HEC-1 model, a 1-D dynamic channel-flow model, a 2-D non-inertia overland-flow model and the SWMM model to reflect the ..
  101. ncbi Specificity of mutations in the PMS2-deficient human tumor cell line HEC-1-A
    T Kato
    Radiation Biology Center, Kyoto University, Kyoto 606, Japan
    Mutat Res 422:279-83. 1998
    ..determined at the hypoxanthine-guanine phosphoribosyltransferase (hprt) locus in the human uterine tumor cell line HEC-1-A which is defective in the mismatch repair gene hPMS2...

Research Grants62

  1. Kinetochore-Microtubule Interactions
    JOHN RICHARD MCINTOSH; Fiscal Year: 2012
    ..microtubule- binding proteins, like the Dam1 complex (aka DASH) from budding yeasts, and fibrous complexes, like NDC80 (Hec1) from C. elegans, yeasts, and human cells...
  2. SACCHAROMYCES CEREVISIAE MICROTUBULE CYTOSKELETON
    Georjana Barnes; Fiscal Year: 2013
    ..with the central kinetochore, or about emergent properties that result when separate subcomplexes like Dam1 and Ndc80 are together in kinetochores...
  3. Cervical/Vaginal Mucus and Microbicides
    Thomas Hope; Fiscal Year: 2012
    ..in semen alone and mixed with mucus and/or microbicide vehicles such as carbopol gel and hydroxy ethyl cellulose (HEC)...
  4. Cervical/Vaginal Mucus and Microbicides
    Thomas Hope; Fiscal Year: 2013
    ..in semen alone and mixed with mucus and/or microbicide vehicles such as carbopol gel and hydroxy ethyl cellulose (HEC)...
  5. Role of the Ndc80 Loop Domain and Cdt1 in Kinetochore Microtubule Attachments
    Dileep Varma; Fiscal Year: 2013
    ..Further, it was demonstrated that the Ndc80 complex recruits Cdt1 to kinetochores through a unique loop domain which was also found to be important for kMT ..
  6. Formin mDia Functions in Mitosis
    Yinghui Mao; Fiscal Year: 2013
    ..of mDia3, along with EB1 and APC, to the microtubule lattice influences the microtubule binding affinity of the Ndc80 complex, which has been implicated as a core microtubule binding force at the kinetochore...
  7. Kinetochore Function in Vertebrate Cells
    Jennifer G DeLuca; Fiscal Year: 2013
    ..The kinetochore-associated NDC80 complex is required for generating stable kinetochore-MT attachments in eukaryotic cells, but how this complex ..
  8. CELL AND TUMOR GENETICS OF MITOTIC ARREST CHECKPOINTS
    PETER KARL SORGER; Fiscal Year: 2010
    ..recruiting Mad and Bub proteins to kinetochores will be studied, with particular emphasis on the members of the Ndc80 complex (4) Bub1, a kinase with dual functions in checkpoint control and chromosome-microtubule attachment will be ..
  9. Architecture-function analysis of the kinetochore motor
    Ajit Joglekar; Fiscal Year: 2013
    ..protein assemblies to determine the necessary and sufficient architectural features of a key kinetochore protein, Ndc80, for reconstituting its distribution and function observed in vivo...
  10. Mechanisms of "End On" Microtubule Attachment by the Kinetochore
    P Todd Stukenberg; Fiscal Year: 2011
    ..We will dissect the role of a microtubule binding activity on the Ndc80/Hec1 subunit of the Ndc80 complex, which is proposed to be an important microtubule interface...
  11. Lymphotoxin: Regulation and Biologic Function
    NANCY RUDDLE; Fiscal Year: 2009
    ..HEVs in lymphangiogenesis mice that express green fluorescent protein under the regulation of a HEV unique gene, HEC-6ST, and mice to be developed that express red fluorescent protein under the regulation of a LV gene, Prox-1, will ..
  12. Biobehavioral Effects of Tai Chi Chih Among Elderly Breast Cancer Survivors
    Anita Y Kinney; Fiscal Year: 2010
    ..As part of this 15-week trial, participants will be randomized to a TCC or a health education control group (HEC)...
  13. Regulation of the kinetochore-microtuble interface
    Walter Gall; Fiscal Year: 2006
    ..These studies show that Ipl1 phosphorylates the highly conserved outer kinetochore component Ndc80 (human Hec1 ortholog) and a MT-associated protein Dam1 (no known human ortholog)...
  14. LYMPHOTOXIN AND LYMPHOID NEOGENESIS
    Nancy H Ruddle; Fiscal Year: 2010
    ..The mature HEV phenotype, characterized by expression of PNAd, LTbR, and a HEV suIfotransferase (HEC-6ST) depends on signaling through the LTbR in normal and HEC6ST- lacZ mice...
  15. ANGIOTENSIN ANALOGS TO TREAT WOUND HEALING
    Kathleen E Rodgers; Fiscal Year: 2010
    ..In a Phase II SBIR grant, US Biotest developed USB001 (NorLeu3-A(1-7) in 2% HEC and preservatives), an angiotensin peptide formulation appropriate for clinical study...
  16. Kinetochore Specification and Function
    Arshad Desai; Fiscal Year: 2013
    ..The ubiquitously conserved KNL-1/Mis12 complex/Ndc80 complex (KMN) protein network is proposed to play a central role at the kinetochore-microtubule interface...
  17. Non-Canonical NF-kappaB Signaling In Endothelial Cells
    Michael J May; Fiscal Year: 2010
    ..feature of these TLOs is the distinct phenotype of the endothelium that resembles the high endothelial cells (HEC) in normal secondary lymph nodes...
  18. Phosphorothioate Oligonucleotides as Microbicides against HIV Transmission
    Peter D Katsikis; Fiscal Year: 2013
    ..Importantly, we show that PDB is active when formulated in hydroxyethylcellulose (HEC) gel at pH 4.4, survives pH transition to a neutral pH, and in retains its activity in HEC for long periods...
  19. Behavioral Exercise Intervention for Smoking Cessation
    Ana M Abrantes; Fiscal Year: 2010
    ..MlBE) AND 12-week smoking cessation including transdermal nicotine patch or 2) 12-week health education control (HEC) AND 12-week smoking cessation including transdermal nicotine patch...
  20. Aerobic Exercise for Smokers with Depressive Symptomatology
    Ana M Abrantes; Fiscal Year: 2013
    ..a 12- session, health education comparison (HEC) condition. Both conditions will be equated for therapist and participant contact time...
  21. Extension Family Lifestyle Intervention Project (FLIP for Kids)
    David M Janicke; Fiscal Year: 2013
    ..their parent(s) will be randomly assigned to a Parent-Only (PO), Family-Based (FB), or a Health Education Control (HEC) intervention...
  22. Hec1 network as molecular therapeutic targets
    Wen Hwa Lee; Fiscal Year: 2013
    ..Over the past decade, Hec1 (Highly Expressed in Cancer 1), originally discovered by us in 1997, has been increasingly recognized as a key ..
  23. Advanced Optical Methods in Cell Biology
    Rudolf Oldenbourg; Fiscal Year: 2012
    ..and release are important to all eukaryotic cells;for the latter, initial work will be done using yeast GFP tagged Ndc80, a ubiquitous kinetochore protein...
  24. Testing the Efficacy of an Exercise Intervention for Patients with OCD
    Ana M Abrantes; Fiscal Year: 2013
    ..to receive either a 12-week moderate intensity aerobic exercise (AE) intervention or a health education control (HEC) comparison intervention...
  25. High End Computing Resource for Large Memory Data-intensive Biomedical Applicatio
    Darrin M York; Fiscal Year: 2010
    DESCRIPTION (provided by applicant): The procurement of a high end computer (HEC) resource is proposed to meet demands of a broad range of 33 research groups at the University of Minnesota that are supported by 91 currently funded NIH ..
  26. BIOLOGY & BIOCHEMISTRY OF MEMBRANE INTERLEUKIN 1
    Evelyn Kurt Jones; Fiscal Year: 1992
    ..addresses basic questions about the biology and biochemistry of membrane IL-1 using human endothelial cells (HEC) as the prototypical membrane IL-1 producing cells...
  27. Low birth weight, uterine infection, and nitric oxide
    Chandrasekhar Yallampalli; Fiscal Year: 2007
    ..1 will characterize NO production, NO synthase (NOS) enzymes in uterine epithelial cell lines, Ishikawa, RL-95 and HEC-1 cells Sub-aim 1...
  28. SITE AND MECHANISM OF ACTION OF A HEPATOTOXIC PEPTIDE
    THOMAS FOXALL; Fiscal Year: 1990
    ..Isolated hepatic endothelial cells (HEC) and hepatic parenchymal cells (HPC) will be prepared by liver perfusion with collagenase and subsequent density-..
  29. Improved Macaque Safety Model for Topical Microbicides: Post-coital Assessments
    Dorothy L Patton; Fiscal Year: 2011
    ..In addition, we will collect parallel assessments, when mating has occurred with a placebo gel (HEC universal placebo) in place...
  30. MOLECULAR SIGNALS IN MONOCYTE RECRUITMENT
    Robert Gerszten; Fiscal Year: 2000
    ..transduction perturbs endothelial biology very little in vitro, and the transduction of human endothelial cells (HEC) to express functional VCAM-1 has been completed...
  31. RETINOID/RETINOID RECEPTOR IN ESOPHAGEAL CANCER RESPONS
    Xiaochun Xu; Fiscal Year: 2002
    ..important role in esophageal carcinogenesis and that restoration of this receptor may enhance retinoid actions on HEC cells. The hypothesis will be tested by using both cultured HEC cells and tissue specimens...
  32. EXTENSION OF ABAN AYA YOUTH PROJECT
    Brian Flay; Fiscal Year: 2002
    ..SDC), an intensive School/Community Program (S/CP), or a control condition (a Health Enhancement Curriculum [HEC]). The SDC includes information on the risks of the targeted behaviors, decision-making...
  33. LYMPHOTOXIN: REGULATION AND BIOLOGICAL SIGNIFICANCE
    NANCY RUDDLE; Fiscal Year: 2006
    ..of lymphoid chemokines in pit mice and in the generation of the L-selectin ligand by the HEV sulfotransferase, HEC-GlcNAc6ST: and molecular characterization of HEV sulfotransferase regulation...
  34. IMMUNOLOGY AND LIVER METASTASES
    Mark Roh; Fiscal Year: 1993
    ..The specific aims are; 1. Determining the mechanism(s) of Kupffer cell (KC) and hepatic endothelial cell (HEC) tumor cytotoxicity. 2...
  35. Role of Nuf2 in Kinetochore Function
    Jennifer DeLuca; Fiscal Year: 2004
    ..abstract_text> ..
  36. Conference: Molecular Mechanism of Leukocyte Trafficking
    Steven Rosen; Fiscal Year: 2002
    ..The resulting interactions are likely to stimulate new approaches to the critical remaining problems in trafficking research. ..
  37. Role of Heparan Sulfate-Degrading Sulfatases in Pancreatic Adenocarcinomas
    Steven Rosen; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  38. CELL SURFACE LECTINS AND INTERCELLULAR ADHESION
    Steven Rosen; Fiscal Year: 2009
    ..As the molecular machinery of normal lymphocyte homing is elucidated, new targets for anti-inflammatory therapeutics are likely to emerge. ..
  39. Chemokine Blockade to Preserve Lung Development
    Richard Auten; Fiscal Year: 2005
    ..Aim 3 will determine whether blockade of leukocyte function can safely preserve normal alveolar development during recovery from severe oxidant stress. ..
  40. Role of Immunization Site in Eliciting Mucosal Immunity
    Peter Anton; Fiscal Year: 2004
    ....
  41. Sleep Disturbances in People with Cancer and Caregivers
    Ann Berger; Fiscal Year: 2004
    ..abstract_text> ..
  42. NEUROBIOLOGICAL FACTORS IN ADOLESCENT ALCOHOL DRINKING
    William McBride; Fiscal Year: 2009
    ..Such information could be helpful in developing useful strategies for combating the major problem of adolescent alcohol drinking. ..
  43. Involvement of Aldehydes in Alcohol Addiction
    William McBride; Fiscal Year: 2002
    ..Such results could provide valuable information on novel mechanisms underlying the addictive properties of alcohol and would provide a firm scientific foundation for future neurobiological studies. ..
  44. IMPACT OF CO-RECEPTOR AND HIV VIRAL BURDEN ON GUT MUCOSA
    Peter Anton; Fiscal Year: 2004
    ..This will be done by looking for decreased mucosal viral burden and evidence of immune reconstitution after therapeutic intervention. ..
  45. In Vitro Cancer Prevention Biomarker Development
    Eugene Elmore; Fiscal Year: 2004
    We have developed and applied an in vitro assay (The Human Epidermal Cell (HEC) Assay) to screen candidate chemopreventive agents for efficacy (Elmore et al., 1999, Elmore et al., 2000)...
  46. Safety and Efficacy of C31G for Ocular Infections
    Richard Bax; Fiscal Year: 2003
    ..Upon achievement of these specific aims, the lead formulation will be advanced through preclinical FDA required studies via a Phase II SBIR. ..
  47. MECHANISMS OF MITOTIC SPINDLE ASSEMBLY AND FUNCTION
    EDWARD SALMON; Fiscal Year: 2003
    ..Finally, we will analyze how MT dynamics may be involved in the regulation of cytokinesis. ..
  48. Internet Use and HIV Risk among Men in NYC
    Alex Carballo Dieguez; Fiscal Year: 2005
    ..abstract_text> ..
  49. TRANSCRIPTOME OF METASTATIC PROSTATE CANCER
    Gennadi Glinsky; Fiscal Year: 2005
    ..Subsequently the expression profile of these protein products will be studied in human prostate tumor samples by immunofluorescence analysis and immunohistochemistry to better correlate gene expression changes with phenotype. ..
  50. VIRULENCE FACTORS OF CHLAMYDIAE
    PRISCILLA WYRICK; Fiscal Year: 2009
    ....
  51. Involvement of Aldehydes in Alcohol Addiction
    William McBride; Fiscal Year: 2009
    ..Understanding the contribution of ACD and THIQs to high alcohol drinking could lead to the development of novel pharmaco-therapies for the treatment of alcoholism and alcohol abuse ..
  52. Neurobiology of high alcohol-seeking behavior
    WILLIAM JOSEPH MCBRIDE; Fiscal Year: 2010
    ....
  53. Trichomonas Lipophosphoglycan in Regulation Immune Responses
    Raina Fichorova; Fiscal Year: 2008
    ..vaginalis infections and complications. [unreadable] [unreadable] [unreadable]..
  54. Mechanisms of Progesterone Receptor Action in Endometriosis
    JI YONG JULIE KIM; Fiscal Year: 2010
    ..These aims will increase the understanding of progesterone resistance in endometriosis as well as investigate the use of targeted biological agents as an alternate treatment for endometriosis. ..
  55. Nuclear envelope membrane proteins and nuclear structure
    Megan King; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  56. FLUORESCENT SPECKLE MICROSCOPY
    EDWARD SALMON; Fiscal Year: 2007
    ..Experience gained in the course of these studies will be used to direct and refine hardware and software development. [unreadable] [unreadable]..
  57. Peer Intervention as Pedagogic Paradigm in Kinshasa SPH
    Frieda Behets; Fiscal Year: 2006
    ..Main outcome measures will be self reported sexual risk behaviors and STI/HIV incidence. The work will inform HIV prevention policy and strengthen research and training at KSPH ..
  58. Topical Microbicide Acceptability
    Alex Carballo Dieguez; Fiscal Year: 2006
    ..abstract_text> ..
  59. Fatigue & Breast Cancer-A Behavioral Sleep Intervention
    Ann Berger; Fiscal Year: 2006
    ..Statistical analyses include RM-ANOVA, generalized estimation equation methodology and multiple regression analysis. Results may inform development of clinical guidelines for fatigue management during adjuvant CT. ..
  60. Predictors of HIV Risk Behavior Among Men in Argentina
    Alex Carballo Dieguez; Fiscal Year: 2009
    ..This is a fundamental step prior to designing programs that mitigate those factors and result in decreased risk. ..