Gene Symbol: Nck
Description: NCK adaptor protein 1
Alias: NCK, NCKalpha, nck-1, cytoplasmic protein NCK1, NCK tyrosine kinase, SH2/SH3 adaptor protein NCK-alpha, melanoma NCK protein, non-catalytic region of tyrosine kinase
Species: human
Products:     Nck

Top Publications

  1. Gruenheid S, DeVinney R, Bladt F, Goosney D, Gelkop S, Gish G, et al. Enteropathogenic E. coli Tir binds Nck to initiate actin pedestal formation in host cells. Nat Cell Biol. 2001;3:856-9 pubmed
    ..Here we show that tyrosine 474 of Tir directly binds the host-cell adaptor protein Nck, and that Nck is required for the recruitment of both neural Wiskott-Aldrich-syndrome protein (N-WASP) and the ..
  2. Anton I, Lu W, Mayer B, Ramesh N, Geha R. The Wiskott-Aldrich syndrome protein-interacting protein (WIP) binds to the adaptor protein Nck. J Biol Chem. 1998;273:20992-5 pubmed
    b>Nck is a ubiquitous adaptor molecule composed of three Src homology 3 (SH3) domains followed by a single SH2 domain...
  3. Kremer B, Adang L, Macara I. Septins regulate actin organization and cell-cycle arrest through nuclear accumulation of NCK mediated by SOCS7. Cell. 2007;130:837-50 pubmed
    ..We now show that this phenotype is induced by nuclear accumulation of the adaptor protein NCK, as the effects can be reversed or induced by cytoplasmic or nuclear NCK, respectively...
  4. Guan S, Fan J, Han A, Chen M, Woodley D, Li W. Non-compensating roles between Nckalpha and Nckbeta in PDGF-BB signaling to promote human dermal fibroblast migration. J Invest Dermatol. 2009;129:1909-20 pubmed publisher
    ..The two closely related SH2/SH3 adapter proteins, Nckalpha and Nckbeta, connect PDGF-BB signaling to the actin cytoskeleton and cell motility...
  5. Li B, Pi Z, Liu L, Zhang B, Huang X, Hu P, et al. FGF-2 prevents cancer cells from ER stress-mediated apoptosis via enhancing proteasome-mediated Nck degradation. Biochem J. 2013;452:139-45 pubmed publisher
    ..Finally, we demonstrated that FGF-2 promoted proteasome-mediated degradation of Nck (non-catalytic region of tyrosine kinase adaptor protein), an SH (Src homology) 2/SH3-containing adaptor protein...
  6. Bokoch G, Wang Y, Bohl B, Sells M, Quilliam L, Knaus U. Interaction of the Nck adapter protein with p21-activated kinase (PAK1). J Biol Chem. 1996;271:25746-9 pubmed
    ..We describe here a specific interaction of the Nck adapter molecule with PAK1 both in vitro and in vivo...
  7. Matuoka K, Miki H, Takahashi K, Takenawa T. A novel ligand for an SH3 domain of the adaptor protein Nck bears an SH2 domain and nuclear signaling motifs. Biochem Biophys Res Commun. 1997;239:488-92 pubmed
    b>Nck is a small protein composed of Src homology regions (SH) 2 and 3, paralleling the adaptors c-Crk and Grb2/Ash, but its function remains enigmatic...
  8. Chen M, She H, Davis E, Spicer C, Kim L, Ren R, et al. Identification of Nck family genes, chromosomal localization, expression, and signaling specificity. J Biol Chem. 1998;273:25171-8 pubmed
    ..Here we report identification of novel human and mouse Nck genes and rename them as the Nckalpha and Nckbeta genes (including the human Nckalpha, human Nckbeta, mouse Nckalpha, and mouse Nckbeta genes)...
  9. Rohatgi R, Nollau P, Ho H, Kirschner M, Mayer B. Nck and phosphatidylinositol 4,5-bisphosphate synergistically activate actin polymerization through the N-WASP-Arp2/3 pathway. J Biol Chem. 2001;276:26448-52 pubmed
    ..The Nck Src homology (SH) 2/3 adaptor binds via its SH3 domains to a proline-rich region on WASP and N-WASP and has been ..

More Information

Publications106 found, 100 shown here

  1. Scaplehorn N, Holmström A, Moreau V, Frischknecht F, Reckmann I, Way M. Grb2 and Nck act cooperatively to promote actin-based motility of vaccinia virus. Curr Biol. 2002;12:740-5 pubmed
    ..studies have shown that N-WASP is required for the actin-based motility of vaccinia virus and is recruited via Nck and WIP. We now show that Grb2 is an additional component of the vaccinia actin tail-forming complex...
  2. Gil D, Schamel W, Montoya M, Sanchez Madrid F, Alarcon B. Recruitment of Nck by CD3 epsilon reveals a ligand-induced conformational change essential for T cell receptor signaling and synapse formation. Cell. 2002;109:901-12 pubmed
    ..change that exposes a proline-rich sequence in CD3 epsilon and results in recruitment of the adaptor protein Nck. This occurs earlier than and independently of tyrosine kinase activation...
  3. Eden S, Rohatgi R, Podtelejnikov A, Mann M, Kirschner M. Mechanism of regulation of WAVE1-induced actin nucleation by Rac1 and Nck. Nature. 2002;418:790-3 pubmed
    ..Here we report a mechanism by which Rac and the adapter protein Nck activate actin nucleation through WAVE1...
  4. Barda Saad M, Braiman A, Titerence R, Bunnell S, Barr V, Samelson L. Dynamic molecular interactions linking the T cell antigen receptor to the actin cytoskeleton. Nat Immunol. 2005;6:80-9 pubmed
    ..Two such adaptor proteins, Nck and the Wiskott-Aldrich syndrome protein (WASp), were recruited to the TCR during initial T cell activation, where ..
  5. Latreille M, Larose L. Nck in a complex containing the catalytic subunit of protein phosphatase 1 regulates eukaryotic initiation factor 2alpha signaling and cell survival to endoplasmic reticulum stress. J Biol Chem. 2006;281:26633-44 pubmed
    ..Here, we demonstrate that the Src homology (SH) domain-containing adaptor Nck is a key component of a molecular complex that controls eIF2alpha phosphorylation and signaling in response to ER ..
  6. Barda Saad M, Shirasu N, Pauker M, Hassan N, Perl O, Balbo A, et al. Cooperative interactions at the SLP-76 complex are critical for actin polymerization. EMBO J. 2010;29:2315-28 pubmed publisher
    ..Generation of a complex of SLP-76, Nck and VAV1 is crucial for regulation of the actin machinery...
  7. Oser M, Dovas A, Cox D, Condeelis J. Nck1 and Grb2 localization patterns can distinguish invadopodia from podosomes. Eur J Cell Biol. 2011;90:181-8 pubmed publisher
    ..These patterns of Nck1 and Grb2 localization, identified in our study, can be used to sub-classify ventral cell surface degradative structures. ..
  8. Pauker M, Reicher B, Fried S, Perl O, Barda Saad M. Functional cooperation between the proteins Nck and ADAP is fundamental for actin reorganization. Mol Cell Biol. 2011;31:2653-66 pubmed publisher
    ..These depend on the recruitment of the signaling proteins Nck and Wiskott-Aldrich syndrome protein (WASp) to the site of TCR activation and on the functional properties of the ..
  9. Anselmi F, Orlandini M, Rocchigiani M, De Clemente C, Salameh A, Lentucci C, et al. c-ABL modulates MAP kinases activation downstream of VEGFR-2 signaling by direct phosphorylation of the adaptor proteins GRB2 and NCK1. Angiogenesis. 2012;15:187-97 pubmed publisher
    ..These findings suggest that c-ABL function is to establish a correct and tightly controlled response of endothelial cells to VEGF-A during the angiogenic process. ..
  10. Pauker M, Hassan N, Noy E, Reicher B, Barda Saad M. Studying the dynamics of SLP-76, Nck, and Vav1 multimolecular complex formation in live human cells with triple-color FRET. Sci Signal. 2012;5:rs3 pubmed publisher
    ..complex consisting of the adaptor proteins SLP-76 (Src homology 2 domain-containing leukocyte protein of 76 kD), Nck, and the guanine nucleotide exchange factor Vav1 is recruited to the T cell side of the interface with an antigen-..
  11. Lehmann J, Riethmuller G, Johnson J. Nck, a melanoma cDNA encoding a cytoplasmic protein consisting of the src homology units SH2 and SH3. Nucleic Acids Res. 1990;18:1048 pubmed
  12. Rivero Lezcano O, Marcilla A, Sameshima J, Robbins K. Wiskott-Aldrich syndrome protein physically associates with Nck through Src homology 3 domains. Mol Cell Biol. 1995;15:5725-31 pubmed
    ..designed to identify p47nck-Src homology 3 (SH3)-binding molecules, we report the cloning of SAKAP II (Src A box Nck-associated protein II) from an HL60 cDNA expression library...
  13. Bubeck Wardenburg J, Pappu R, Bu J, Mayer B, Chernoff J, Straus D, et al. Regulation of PAK activation and the T cell cytoskeleton by the linker protein SLP-76. Immunity. 1998;9:607-16 pubmed
    ..We demonstrate here that the SLP-76 linker protein interacts with both nck, an adaptor protein, and Vav, a guanine nucleotide exchange factor for Rho-family GTPases...
  14. Braverman L, Quilliam L. Identification of Grb4/Nckbeta, a src homology 2 and 3 domain-containing adapter protein having similar binding and biological properties to Nck. J Biol Chem. 1999;274:5542-9 pubmed
    ..Here we report that Grb4 is an adapter protein closely related to but distinct from Nck that is made up of three SH3 domains and one SH2 domain...
  15. Kebache S, Zuo D, Chevet E, Larose L. Modulation of protein translation by Nck-1. Proc Natl Acad Sci U S A. 2002;99:5406-11 pubmed
    In mammals, Nck represented by two genes, is a 47-kDa SH2/SH3 domain-containing protein lacking intrinsic enzymatic function...
  16. Kebache S, Cardin E, Nguyen D, Chevet E, Larose L. Nck-1 antagonizes the endoplasmic reticulum stress-induced inhibition of translation. J Biol Chem. 2004;279:9662-71 pubmed
    ..Here we show that the Src homology 2/3 (SH2/SH3) domain-containing protein Nck-1 prevents the unfolded protein response normally induced by pharmacological endoplasmic reticulum (ER) stress ..
  17. Jones N, Blasutig I, Eremina V, Ruston J, Bladt F, Li H, et al. Nck adaptor proteins link nephrin to the actin cytoskeleton of kidney podocytes. Nature. 2006;440:818-23 pubmed
    ..Here we show that nephrin selectively binds the Src homology 2 (SH2)/SH3 domain-containing Nck adaptor proteins, which in turn control the podocyte cytoskeleton in vivo...
  18. Verma R, Kovari I, Soofi A, Nihalani D, Patrie K, Holzman L. Nephrin ectodomain engagement results in Src kinase activation, nephrin phosphorylation, Nck recruitment, and actin polymerization. J Clin Invest. 2006;116:1346-59 pubmed
    ..that this nephrin phosphorylation event resulted in recruitment of the SH2-SH3 domain-containing adapter protein Nck and assembly of actin filaments in an Nck-dependent fashion...
  19. Ger M, Zitkus Z, Valius M. Adaptor protein Nck1 interacts with p120 Ras GTPase-activating protein and regulates its activity. Cell Signal. 2011;23:1651-8 pubmed publisher
    ..This leads to the complex dissipation, decrease of RasGAP activity and the increase of H-Ras-GTP level in the detached cells. Our findings reveal unexpected feature of adaptor protein Nck1 as the regulator of RasGAP activity. ..
  20. Wu C, Buszard B, Teng C, Chen W, Warr C, Tiganis T, et al. Dock/Nck facilitates PTP61F/PTP1B regulation of insulin signalling. Biochem J. 2011;439:151-9 pubmed publisher
    ..establish the role of the SH3 (Src homology 3)/SH2 adaptor protein Dock (Dreadlocks) and its mammalian counterpart Nck in IR regulation by PTPs...
  21. Yiemwattana I, Ngoenkam J, Paensuwan P, Kriangkrai R, Chuenjitkuntaworn B, Pongcharoen S. Essential role of the adaptor protein Nck1 in Jurkat T cell activation and function. Clin Exp Immunol. 2012;167:99-107 pubmed publisher
    The non-catalytic region of tyrosine kinase (Nck) is proposed to play an essential role in T cell activation. However, evidence based on functional and biochemical studies has brought into question the critical function of Nck...
  22. Buvall L, Rashmi P, López Rivera E, Andreeva S, Weins A, Wallentin H, et al. Proteasomal degradation of Nck1 but not Nck2 regulates RhoA activation and actin dynamics. Nat Commun. 2013;4:2863 pubmed publisher
    ..These findings reveal proteasomal regulation as a key factor in the distinct and non-redundant effects of Nck on RhoA-mediated actin dynamics.
  23. Fu C, Turck C, Kurosaki T, Chan A. BLNK: a central linker protein in B cell activation. Immunity. 1998;9:93-103 pubmed
    ..receptor-associated Syk tyrosine kinase with PLCgamma, the Vav guanine nucleotide exchange factor, and the Grb2 and Nck adapter proteins...
  24. Murakami H, Yamamura Y, Shimono Y, Kawai K, Kurokawa K, Takahashi M. Role of Dok1 in cell signaling mediated by RET tyrosine kinase. J Biol Chem. 2002;277:32781-90 pubmed
    ..Analysis by site-directed mutagenesis revealed that tyrosine 361 in mouse Dok1 represents a binding site for the Nck adaptor protein and tyrosines 295, 314, 361, 376, 397, and 408 for the Ras-GTPase-activating protein...
  25. Alan J, Struckhoff E, Lundquist E. Multiple cytoskeletal pathways and PI3K signaling mediate CDC-42-induced neuronal protrusion in C. elegans. Small Gtpases. 2013;4:208-20 pubmed publisher
    ..We also identified the Nck-interacting kinase MIG-15/NIK and p21-activated kinases (PAKs), also implicated in axon guidance...
  26. Nieto Pelegrin E, Kenny B, Martinez Quiles N. Nck adaptors, besides promoting N-WASP mediated actin-nucleation activity at pedestals, influence the cellular levels of enteropathogenic Escherichia coli Tir effector. Cell Adh Migr. 2014;8:404-17 pubmed publisher
    ..Tir-Intimin interaction recruits the Nck adaptor to a Tir tyrosine phosphorylated residue where it activates neural Wiskott-Aldrich syndrome protein (N-WASP)..
  27. Master Z, Jones N, Tran J, Jones J, Kerbel R, Dumont D. Dok-R plays a pivotal role in angiopoietin-1-dependent cell migration through recruitment and activation of Pak. EMBO J. 2001;20:5919-28 pubmed ECs following Ang1 stimulation, resulting in tyrosine phosphorylation of Dok-R and the subsequent recruitment of Nck and the p21-activating kinase (Pak/Pak1) to the activated receptor...
  28. Tong H, Jiang R, Zhang W, Yan Y. MiR-2425-5p targets RAD9A and MYOG to regulate the proliferation and differentiation of bovine skeletal muscle-derived satellite cells. Sci Rep. 2017;7:418 pubmed publisher
    ..In addition, we found that miR-2425-5p expression was regulated by its host gene NCK associated protein 5-like (NCKAP5L) rather than being transcribed independently as a separate small RNA...
  29. Wenzel J, Sanzenbacher R, Ghadimi M, Lewitzky M, Zhou Q, Kaplan D, et al. Multiple interactions of the cytosolic polyproline region of the CD95 ligand: hints for the reverse signal transduction capacity of a death factor. FEBS Lett. 2001;509:255-62 pubmed
    ..These results may help to explain the costimulatory capacity of CD95L. ..
  30. Konakahara S, Suzuki Y, Kawakami T, Saitou M, Kajikawa M, Masuho Y, et al. A neuronal transmembrane protein LRFN4 complexes with 14-3-3s and NCK1 to induce morphological change in monocytic cells via Rac1-mediated actin cytoskeleton reorganization. FEBS Lett. 2012;586:2251-9 pubmed publisher
    ..Here, we examined a molecular mechanism regulating LRFN4-mediated cell elongation. We found that 14-3-3 and NCK proteins complexed with LRFN4, and they were involved in LRFN4-mediated cell elongation...
  31. Cohen J, Ruppert A, Heerema N, Andritsos L, Jones J, Porcu P, et al. Complex karyotype is associated with aggressive disease and shortened progression-free survival in patients with newly diagnosed mantle cell lymphoma. Clin Lymphoma Myeloma Leuk. 2015;15:278-285.e1 pubmed publisher
    ..Demographic, clinical, and survival differences between patients with CK and non-CK (NCK) were assessed. Of 80 patients, 32 (40%) had CK, which was associated with high-risk clinical risk factors...
  32. Shishido T, Akagi T, Ouchi T, Georgescu M, Langdon W, Hanafusa H. The kinase-deficient Src acts as a suppressor of the Abl kinase for Cbl phosphorylation. Proc Natl Acad Sci U S A. 2000;97:6439-44 pubmed
    ..This results in a ternary complex that inhibits the Abl-mediated phosphorylation of Cbl by steric hindrance. These results illustrate a mechanism by which the enzymatically inactive Src can exert a biological function in vivo. ..
  33. Jia C, Nie J, Wu C, Li C, Li S. Novel Src homology 3 domain-binding motifs identified from proteomic screen of a Pro-rich region. Mol Cell Proteomics. 2005;4:1155-66 pubmed demonstrated that several SH3 domains, including those from growth factor receptor-bound protein 2 (Grb2), NCK, and phospholipase C (PLC)-gamma1, bound with moderate affinities (10-100 microm) to a group of non-conventional ..
  34. Hu Q, Milfay D, Williams L. Binding of NCK to SOS and activation of ras-dependent gene expression. Mol Cell Biol. 1995;15:1169-74 pubmed
    b>NCK, an SH2- and SH3 domain-containing protein, becomes phosphorylated and associated with tyrosine kinase receptors upon growth factor stimulation...
  35. Hummel T, Leifker K, Klämbt C. The Drosophila HEM-2/NAP1 homolog KETTE controls axonal pathfinding and cytoskeletal organization. Genes Dev. 2000;14:863-73 pubmed
    ..In vitro analysis has shown a specific interaction of the vertebrate HEM-2/NAP1 with the SH2-SH3 adapter protein NCK and the small GTPase RAC1, which both have been implicated in regulating cytoskeleton organization and axonal ..
  36. Kefalas G, Jouvet N, Baldwin C, Estall J, Larose L. Peptide-based sequestration of the adaptor protein Nck1 in pancreatic β cells enhances insulin biogenesis and protects against diabetogenic stresses. J Biol Chem. 2018;293:12516-12524 pubmed publisher
    ..Herein, we explored the therapeutic potential of abrogating the interaction between Nck and PERK to improve β-cell function and survival...
  37. Kinley A, Weed S, Weaver A, Karginov A, Bissonette E, Cooper J, et al. Cortactin interacts with WIP in regulating Arp2/3 activation and membrane protrusion. Curr Biol. 2003;13:384-93 pubmed
    ..These data suggest that a cortactin-WIP complex functions in regulating actin-based structures at the cell periphery. ..
  38. Pauker M, Reicher B, Joseph N, Wortzel I, Jakubowicz S, Noy E, et al. WASp family verprolin-homologous protein-2 (WAVE2) and Wiskott-Aldrich syndrome protein (WASp) engage in distinct downstream signaling interactions at the T cell antigen receptor site. J Biol Chem. 2014;289:34503-19 pubmed publisher
    ..WAVE2 recruitment to the TCR site depends on protein-tyrosine kinase, ZAP-70, and the adaptors LAT, SLP-76, and Nck. However, in contrast to WASp, WAVE2 leaves this signaling complex and migrates peripherally together with vinculin ..
  39. Lee Y, Jung J, Park K, Kim S, Kim J. Synergistic inhibition effect of TNIK inhibitor KY-05009 and receptor tyrosine kinase inhibitor dovitinib on IL-6-induced proliferation and Wnt signaling pathway in human multiple myeloma cells. Oncotarget. 2017;8:41091-41101 pubmed publisher
    ..investigated the relationship between interleukin-6-induced proliferation of multiple myeloma cells and Traf2- and Nck-interacting kinase (TNIK) expression in Wnt signaling...
  40. Coba M, Komiyama N, Nithianantharajah J, Kopanitsa M, Indersmitten T, Skene N, et al. TNiK is required for postsynaptic and nuclear signaling pathways and cognitive function. J Neurosci. 2012;32:13987-99 pubmed publisher
    Traf2 and NcK interacting kinase (TNiK) contains serine-threonine kinase and scaffold domains and has been implicated in cell proliferation and glutamate receptor regulation in vitro...
  41. Stephanowitz H, Lange S, Lang D, Freund C, Krause E. Improved two-dimensional reversed phase-reversed phase LC-MS/MS approach for identification of peptide-protein interactions. J Proteome Res. 2012;11:1175-83 pubmed publisher
    ..Tyr 595 of ADAP may serve as a direct binding site for the SH2 domains of the T cell proteins SLP76 and NCK. From a technical point of view we provide a detailed protocol for an offline 2-D RP-RP LC-MS/MS method that offers ..
  42. Wolf I, Rohrschneider L. Fiz1, a novel zinc finger protein interacting with the receptor tyrosine kinase Flt3. J Biol Chem. 1999;274:21478-84 pubmed
    ..Together, these results identified a novel class of interaction between a receptor tyrosine kinase and a signaling molecule which is independent of the well established SH2 domain/phosphotyrosine binding. ..
  43. Vorobieva N, Protopopov A, Protopopova M, Kashuba V, Allikmets R, Modi W, et al. Localization of human ARF2 and NCK genes and 13 other NotI-linking clones to chromosome 3 by fluorescence in situ hybridization. Cytogenet Cell Genet. 1995;68:91-4 pubmed
    Two human genes containing NotI sites, ADP-ribosylation factor (ARF2) and melanoma NCK protein, were mapped by fluorescence in situ hybridization to 3p21.2-->p21.1 and 3q21, respectively...
  44. Tang J, Feng G, Li W. Induced direct binding of the adapter protein Nck to the GTPase-activating protein-associated protein p62 by epidermal growth factor. Oncogene. 1997;15:1823-32 pubmed
    The SH3-SH3-SH3-SH2 adapter protein Nck links receptor tyrosine kinases, such as EGF and PDGF receptors, to downstream signaling pathways, among which p21cdc42/rac-activated kinase cascade, Sos-activated Ras signaling and the human ..
  45. Sauer K, Liou J, Singh S, Yablonski D, Weiss A, Perlmutter R. Hematopoietic progenitor kinase 1 associates physically and functionally with the adaptor proteins B cell linker protein and SLP-76 in lymphocytes. J Biol Chem. 2001;276:45207-16 pubmed
  46. Stoletov K, Gong C, Terman B. Nck and Crk mediate distinct VEGF-induced signaling pathways that serve overlapping functions in focal adhesion turnover and integrin activation. Exp Cell Res. 2004;295:258-68 pubmed
    We have asked whether the Nck and Crk adaptor proteins play important roles in the vascular endothelial growth factor (VEGF)-induced signaling pathways that lead to an enhancement in cell migration...
  47. Cardin E, Latreille M, Khoury C, Greenwood M, Larose L. Nck-1 selectively modulates eIF2alphaSer51 phosphorylation by a subset of eIF2alpha-kinases. FEBS J. 2007;274:5865-75 pubmed
    ..In this article, we report that the ubiquitously expressed adaptor protein Nck, composed only of Src homology domains and classically implicated in cell signaling by activated plasma membrane ..
  48. Qiu F, Huang D, Xiao H, Qiu F, Lu L, Nie J. Detection of tyrosine?phosphorylated proteins in hepatocellular carcinoma tissues using a combination of GST?Nck1?SH2 pull?down and two?dimensional electrophoresis. Mol Med Rep. 2013;7:1209-14 pubmed publisher
    ..We previously identified a group of Nck Src homology 2 (SH2) domain?binding proteins by combining the GST-Nck1-SH2 pull-down method with two-dimensional ..
  49. Humphries A, Donnelly S, Way M. Cdc42 and the Rho GEF intersectin-1 collaborate with Nck to promote N-WASP-dependent actin polymerisation. J Cell Sci. 2014;127:673-85 pubmed publisher
    ..of the viral transmembrane protein A36, leading to recruitment of a signalling network consisting of Grb2, Nck, WIP and N-WASP. Nck is a potent activator of N-WASP-Arp2/3-dependent actin polymerisation...
  50. Sawa M, Masuda M, Yamada T. Targeting the Wnt signaling pathway in colorectal cancer. Expert Opin Ther Targets. 2016;20:419-29 pubmed publisher
    ..TRAF2 and NCK-interacting protein kinase (TNIK) was identified as the most downstream regulator of Wnt signaling by two ..
  51. Yue M, Luo D, Yu S, Liu P, Zhou Q, Hu M, et al. Misshapen/NIK-related kinase (MINK1) is involved in platelet function, hemostasis, and thrombus formation. Blood. 2016;127:927-37 pubmed publisher
    The sterile-20 kinase misshapen/Nck-interacting kinase (NIK)-related kinase 1 (MINK1) is involved in many important cellular processes such as growth, cytoskeletal rearrangement, and motility...
  52. Holland S, Gale N, Gish G, Roth R, Songyang Z, Cantley L, et al. Juxtamembrane tyrosine residues couple the Eph family receptor EphB2/Nuk to specific SH2 domain proteins in neuronal cells. EMBO J. 1997;16:3877-88 pubmed
    ..which in turn formed a complex with the Ras GTPase-activating protein (RasGAP) and SH2/SH3 domain adaptor protein Nck. RasGAP also bound through its SH2 domains to tyrosine-phosphorylated EphB2 in vitro, and complexed with activated ..
  53. Anafi M, Kiefer F, Gish G, Mbamalu G, Iscove N, Pawson T. SH2/SH3 adaptor proteins can link tyrosine kinases to a Ste20-related protein kinase, HPK1. J Biol Chem. 1997;272:27804-11 pubmed
    ..Such interaction may provide a possible mechanism for cross-talk between distinct biochemical pathways following the activation of tyrosine kinases. ..
  54. Tu Y, Kucik D, Wu C. Identification and kinetic analysis of the interaction between Nck-2 and DOCK180. FEBS Lett. 2001;491:193-9 pubmed
    b>Nck-2 is a newly identified adapter protein comprising three N-terminal SH3 domains and one C-terminal SH2 domain...
  55. Lim C, Park E, Kim D, Song Y, Eom S, Chun J, et al. SPIN90 (SH3 protein interacting with Nck, 90 kDa), an adaptor protein that is developmentally regulated during cardiac myocyte differentiation. J Biol Chem. 2001;276:12871-8 pubmed
    In the yeast two-hybrid screening, we have isolated a cDNA clone from a human heart library using Nck Src homology 3 (SH3) domains as bait...
  56. Miyoshi Akiyama T, Aleman L, Smith J, Adler C, Mayer B. Regulation of Cbl phosphorylation by the Abl tyrosine kinase and the Nck SH2/SH3 adaptor. Oncogene. 2001;20:4058-69 pubmed
    ..Cbl and the Abl nonreceptor tyrosine kinase both bind to SH3 domains from the SH2/SH3 adaptor Nck, and are candidate effectors for Nck function...
  57. Stoletov K, Ratcliffe K, Terman B. Fibroblast growth factor receptor substrate 2 participates in vascular endothelial growth factor-induced signaling. FASEB J. 2002;16:1283-5 pubmed
    ..VEGF treatment of KDR-expressing cells leads to the recruitment of Nck, p21-activated kinase, Crk, Grb2, and protein kinase C l to FRS2...
  58. Banjade S, Wu Q, Mittal A, Peeples W, Pappu R, Rosen M. Conserved interdomain linker promotes phase separation of the multivalent adaptor protein Nck. Proc Natl Acad Sci U S A. 2015;112:E6426-35 pubmed publisher
    ..The adaptor protein Nck has three SRC-homology 3 (SH3) domains that bind multiple proline-rich segments in the actin regulatory protein ..
  59. Roche S, McGlade J, Jones M, Gish G, Pawson T, Courtneidge S. Requirement of phospholipase C gamma, the tyrosine phosphatase Syp and the adaptor proteins Shc and Nck for PDGF-induced DNA synthesis: evidence for the existence of Ras-dependent and Ras-independent pathways. EMBO J. 1996;15:4940-8 pubmed
    ..called SH-PTP2), phospholipase C (PLC) gamma1, rasGTPase Activating Protein (rasGAP) and the adapter molecules Nck and Shc in the mitogenic response induced by PDGF in fibroblasts...
  60. Schmitz U, Ishida M, Berk B. Angiotensin II stimulates tyrosine phosphorylation of phospholipase C-gamma-associated proteins. Characterization of a c-Src-dependent 97-kD protein in vascular smooth muscle cells. Circ Res. 1997;81:550-7 pubmed
    ..These studies are the first to define a PLC-gamma-associated protein that may be required for Ang II-mediated signal transduction. ..
  61. Erdreich Epstein A, Liu M, Kant A, Izadi K, Nolta J, Durden D. Cbl functions downstream of Src kinases in Fc gamma RI signaling in primary human macrophages. J Leukoc Biol. 1999;65:523-34 pubmed
    ..tyrosine phosphorylated in response to Fc receptor activation and binds to the adaptor proteins Grb2, CrkL, and Nck. A few reports describe Cbl interactions in primary human hematopoietic cells...
  62. Nagaraja T, Anand A, Zhao H, Ganju R. The adaptor protein SLP-76 regulates HIV-1 release and cell-to-cell transmission in T cells. J Immunol. 2012;188:2769-77 pubmed publisher
    ..Taken together, our studies demonstrate a novel role for the adaptor molecule SLP-76 in regulating HIV-1 infection in T cells with the potential to develop innovative strategies against HIV-1. ..
  63. Ditlev J, Michalski P, Huber G, Rivera G, Mohler W, Loew L, et al. Stoichiometry of Nck-dependent actin polymerization in living cells. J Cell Biol. 2012;197:643-58 pubmed publisher
    Regulation of actin dynamics through the Nck/N-WASp (neural Wiskott-Aldrich syndrome protein)/Arp2/3 pathway is essential for organogenesis, cell invasiveness, and pathogen infection...
  64. Paensuwan P, Hartl F, Yousefi O, Ngoenkam J, Wipa P, Beck Garcia E, et al. Nck Binds to the T Cell Antigen Receptor Using Its SH3.1 and SH2 Domains in a Cooperative Manner, Promoting TCR Functioning. J Immunol. 2016;196:448-58 pubmed publisher
    ..1 domain of the adaptor molecule noncatalytic region of tyrosine kinase (Nck) can bind to the exposed PRS of CD3ε, but the molecular mechanism of how full-length Nck binds to the TCR in ..
  65. Zhou T, Liu S, Geng X, Jin Y, Jiang C, Bao L, et al. GWAS analysis of QTL for enteric septicemia of catfish and their involved genes suggest evolutionary conservation of a molecular mechanism of disease resistance. Mol Genet Genomics. 2017;292:231-242 pubmed publisher
    ..The fact that nck1 is mapped in the QTL and that it was significantly upregulated in channel catfish intestine after ESC challenge suggested its candidacy of being involved in resistance/susceptibility of ESC. ..
  66. Porciello N, Grazioli P, Campese A, Kunkl M, Caristi S, Mastrogiovanni M, et al. A non-conserved amino acid variant regulates differential signalling between human and mouse CD28. Nat Commun. 2018;9:1080 pubmed publisher
    ..Moreover, this Y209APP212 sequence in humans is crucial for the association of CD28 with the Nck adaptor protein for actin cytoskeleton reorganisation events necessary for CD28 autonomous signalling...
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    b>Nck, an adaptor protein composed of one SH2 and three SH3 domains, is a common target for a variety of cell surface receptors...
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    cDNAs for the Na+/Ca2+ exchanger from Drosophila melanogaster (Dmel/Nck) have been cloned by homology screening using the human heart Na+/Ca2+ exchanger cDNA...
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    ..The strongest interactions were seen with the SH2-SH3 adapter proteins Grb2 and Nck. The repeat sequence itself is not essential in this interaction...
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    One of the adaptor proteins, Nck, comprises a single SH2 domain and three SH3 domains that are important in protein-protein interactions...
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    ..Upon TCR/CD3 stimulation, HPK1 formed inducible complexes with the adapters Nck and Crk with different kinetics, whereas it constitutively interacted with the adapters Grb2 and CrkL in Jurkat T ..
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    ..Human Nap1 proved to be an orthologue of rat Nap1 which binds to the adaptor molecule Nck in signal transduction...
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    b>Nck is an adaptor protein composed of three N-terminal Src Homology (SH) 3 domains followed by a unique C?terminal SH2 domain...
  74. Kim D, Kang M, Kim C, Huh Y, Cho I, Ryu H, et al. SPIN90 Modulates Long-Term Depression and Behavioral Flexibility in the Hippocampus. Front Mol Neurosci. 2017;10:295 pubmed publisher
    ..SH3 protein interacting with Nck, 90 kDa (SPIN90), an Nck-interacting protein highly expressed in synapses, is essential for actin remodeling and ..
  75. Li W, Hu P, Skolnik E, Ullrich A, Schlessinger J. The SH2 and SH3 domain-containing Nck protein is oncogenic and a common target for phosphorylation by different surface receptors. Mol Cell Biol. 1992;12:5824-33 pubmed
    ..b>Nck is an ubiquitously expressed protein consisting exclusively of one SH2 and three SH3 domains...
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    We have previously shown that overexpression of the SH2- and SH3-containing Nck adaptor protein causes transformation of mammalian fibroblast...
  77. Quilliam L, Lambert Q, Mickelson Young L, Westwick J, Sparks A, Kay B, et al. Isolation of a NCK-associated kinase, PRK2, an SH3-binding protein and potential effector of Rho protein signaling. J Biol Chem. 1996;271:28772-6 pubmed
    The NCK adapter protein is comprised of three consecutive Src homology 3 (SH3) protein-protein interaction domains and a C-terminal SH2 domain...
  78. Noguchi T, Matozaki T, Inagaki K, Tsuda M, Fukunaga K, Kitamura Y, et al. Tyrosine phosphorylation of p62(Dok) induced by cell adhesion and insulin: possible role in cell migration. EMBO J. 1999;18:1748-60 pubmed
    ..Insulin promoted the association of tyrosine-phosphorylated Dok with the adapter protein NCK and rasGAP...
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    The Nck adaptor protein comprises a single C-terminal SH2 domain and three SH3 domains. The domain structure of Nck suggests that Nck links tyrosine kinase substrates to proteins containing proline-rich motifs...
  80. Lock P, Casagranda F, Dunn A. Independent SH2-binding sites mediate interaction of Dok-related protein with RasGTPase-activating protein and Nck. J Biol Chem. 1999;274:22775-84 pubmed
    ..on tyrosine residues and consequential physical association with RasGTPase-activating protein (GAP) and the Nck adaptor protein...
  81. Jones N, Dumont D. Recruitment of Dok-R to the EGF receptor through its PTB domain is required for attenuation of Erk MAP kinase activation. Curr Biol. 1999;9:1057-60 pubmed
    ..GTPase-activating protein rasGAP and Tyr351 mediates an association with the SH2 domain of the adapter protein Nck. Interestingly, we have found that Dok-R could attenuate EGF-stimulated mitogen-activated protein (MAP) kinase ..
  82. Adler C, Miyoshi Akiyama T, Aleman L, Tanaka M, Smith J, Mayer B. Abl family kinases and Cbl cooperate with the Nck adaptor to modulate Xenopus development. J Biol Chem. 2000;275:36472-8 pubmed
    We previously showed that overexpression of the Nck Src homology (SH) 2/SH3 adaptor in Xenopus embryos induced developmental defects including anterior truncation and mesoderm ventralization...
  83. Pedraza Alva G, Sawasdikosol S, Liu Y, Mérida L, Cruz Muñoz M, Oceguera Yanez F, et al. Regulation of Cbl molecular interactions by the co-receptor molecule CD43 in human T cells. J Biol Chem. 2001;276:729-37 pubmed
    ..These data suggest that by inducing its phosphorylation on serine residues, CD43-mediated signals may regulate the molecular associations and functions of the Cbl adapter protein. ..
  84. Stoletov K, Ratcliffe K, Spring S, Terman B. NCK and PAK participate in the signaling pathway by which vascular endothelial growth factor stimulates the assembly of focal adhesions. J Biol Chem. 2001;276:22748-55 pubmed
    ..VEGF stimulated the tyrosine phosphorylation of the SH2 domain-containing signaling proteins NCK and CRK in human umbilical vein endothelial cells...
  85. Ratcliffe K, Tao Q, Yavuz B, Stoletov K, Spring S, Terman B. Sck is expressed in endothelial cells and participates in vascular endothelial growth factor-induced signaling. Oncogene. 2002;21:6307-16 pubmed
    ..Taken together, these results demonstrate that Sck is expressed in vascular endothelial cells, and participates in VEGF-induced signal transduction. ..
  86. Hu Y, Leo C, Yu S, Huang B, Wang H, Shen M, et al. Identification and functional characterization of a novel human misshapen/Nck interacting kinase-related kinase, hMINK beta. J Biol Chem. 2004;279:54387-97 pubmed
    ..hMINK beta is ubiquitously expressed in most tissues with at least five alternatively spliced isoforms. Similar to Nck interacting kinase (NIK) and Traf2 and Nck-interacting kinase (TNIK), hMINK beta moderately activates c-Jun N-..
  87. Kesti T, Ruppelt A, Wang J, Liss M, Wagner R, Tasken K, et al. Reciprocal regulation of SH3 and SH2 domain binding via tyrosine phosphorylation of a common site in CD3epsilon. J Immunol. 2007;179:878-85 pubmed
    ..Studies with recombinant proteins confirmed strong binding of CD3epsilon to Eps8L1 and Nck SH3 domains...
  88. Hem C, Sundvold Gjerstad V, Granum S, Koll L, Abrahamsen G, Buday L, et al. T cell specific adaptor protein (TSAd) promotes interaction of Nck with Lck and SLP-76 in T cells. Cell Commun Signal. 2015;13:31 pubmed publisher
    ..the Src homology 2 (SH2) domain of the actin regulator Non-catalytic region of tyrosine kinase adaptor protein (Nck) potentially binds to TSAd phosphorylated on Tyr(280) (pTyr(280)) and pTyr(305)...
  89. Li H, Li B, Larose L. IRE1? links Nck1 deficiency to attenuated PTP1B expression in HepG2 cells. Cell Signal. 2017;36:79-90 pubmed publisher
    ..Overall, this study highlights an important role for Nck1 in fine-tuning IRE1? expression and signaling that regulate PTP1B expression and subsequent activation of the PI3K-Akt pathway in HepG2 cells. ..
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    ..coimmunoprecipitated with phospholipase C (PLC)-gamma 1 by anti-PLC-gamma 1 monoclonal antibodies is proved to be Nck, a protein composed almost exclusively of one SH2 and three SH3 domains...
  91. Stein E, Huynh Do U, Lane A, Cerretti D, Daniel T. Nck recruitment to Eph receptor, EphB1/ELK, couples ligand activation to c-Jun kinase. J Biol Chem. 1998;273:1303-8 pubmed
    ..Six independent cDNAs encoding the SH2 domain of the adapter protein, Nck, were recovered in a screen of a murine embryonic library...
  92. Zhao C, Ma H, Bossy Wetzel E, Lipton S, Zhang Z, Feng G. GC-GAP, a Rho family GTPase-activating protein that interacts with signaling adapters Gab1 and Gab2. J Biol Chem. 2003;278:34641-53 pubmed
    ..contains several classic proline-rich motifs, and it interacts with the first SH3 domain of Crk and full-length Nck in vitro...