Gene Symbol: NASP
Description: nuclear autoantigenic sperm protein
Alias: FLB7527, HMDRA1, PRO1999, nuclear autoantigenic sperm protein, NASP histone chaperone, histone H1-binding protein, nuclear autoantigenic sperm protein (histone-binding)
Species: human
Products:     NASP

Top Publications

  1. Welch J, Zimmerman L, Joseph D, O Rand M. Characterization of a sperm-specific nuclear autoantigenic protein. I. Complete sequence and homology with the Xenopus protein, N1/N2. Biol Reprod. 1990;43:559-68 pubmed
    ..This nuclear autoantigenic sperm protein (NASP) contains a C-terminal nuclear translocation signal and has structural similarities to the ..
  2. Finn R, Ellard K, Eirín López J, Ausio J. Vertebrate nucleoplasmin and NASP: egg histone storage proteins with multiple chaperone activities. FASEB J. 2012;26:4788-804 pubmed publisher
    ..NPM2/NPM3), which are preferentially associated with histones H2A-H2B in the egg and the nuclear autoantigenic sperm protein (NASP) families...
  3. Cook A, Gurard Levin Z, Vassias I, Almouzni G. A specific function for the histone chaperone NASP to fine-tune a reservoir of soluble H3-H4 in the histone supply chain. Mol Cell. 2011;44:918-27 pubmed publisher
    ..Here, we reveal that the histone chaperone nuclear autoantigenic sperm protein (NASP) protects a reservoir of soluble histones H3-H4...
  4. Ali Fehmi R, Chatterjee M, Ionan A, Levin N, Arabi H, Bandyopadhyay S, et al. Analysis of the expression of human tumor antigens in ovarian cancer tissues. Cancer Biomark. 2010;6:33-48 pubmed publisher
    ..The protein expression patterns of 4 of those 65 antigens, namely NASP, RCAS1, Nijmegen breakage syndrome1 (NBS1) and eIF5A, along with p53 and Her2 (known molecular prognosticators) and ..
  5. Tagami H, Ray Gallet D, Almouzni G, Nakatani Y. Histone H3.1 and H3.3 complexes mediate nucleosome assembly pathways dependent or independent of DNA synthesis. Cell. 2004;116:51-61 pubmed
    ..This finding provides new insights into possible mechanisms for maintenance of epigenetic information after chromatin duplication. ..
  6. Batova I, O Rand M. Histone-binding domains in a human nuclear autoantigenic sperm protein. Biol Reprod. 1996;54:1238-44 pubmed
    In one of our previous studies, the deduced amino acid sequence of the human nuclear autoantigenic sperm protein (hNASP) revealed two conserved histone-binding domains when compared to the Xenopus N1/N2 protein sequence...
  7. Kleinschmidt J, Dingwall C, Maier G, Franke W. Molecular characterization of a karyophilic, histone-binding protein: cDNA cloning, amino acid sequence and expression of nuclear protein N1/N2 of Xenopus laevis. EMBO J. 1986;5:3547-52 pubmed
    ..abstract truncated at 250 words) ..
  8. Richardson R, Batova I, Widgren E, Zheng L, Whitfield M, Marzluff W, et al. Characterization of the histone H1-binding protein, NASP, as a cell cycle-regulated somatic protein. J Biol Chem. 2000;275:30378-86 pubmed
    b>Nuclear autoantigenic sperm protein (NASP), initially described as a highly autoimmunogenic testis and sperm-specific protein, is a histone-binding protein that is a homologue of the N1/N2 gene expressed in oocytes of Xenopus laevis...
  9. O Rand M, Richardson R, Zimmerman L, Widgren E. Sequence and localization of human NASP: conservation of a Xenopus histone-binding protein. Dev Biol. 1992;154:37-44 pubmed
    In this study the sequence and localization of human testicular NASP (nuclear autoantigenic sperm protein) are reported. NASP cDNA contains 2561 nt encoding a protein of 787 amino acids...

More Information


  1. Alekseev O, Bencic D, Richardson R, Widgren E, O Rand M. Overexpression of the Linker histone-binding protein tNASP affects progression through the cell cycle. J Biol Chem. 2003;278:8846-52 pubmed
    b>NASP is an H1 histone-binding protein that is cell cycle-regulated and occurs in two major forms: tNASP, found in gametes, embryonic cells, and transformed cells; and sNASP, found in all rapidly dividing somatic cells (Richardson, R. T...
  2. Richardson R, Alekseev O, Grossman G, Widgren E, Thresher R, Wagner E, et al. Nuclear autoantigenic sperm protein (NASP), a linker histone chaperone that is required for cell proliferation. J Biol Chem. 2006;281:21526-34 pubmed
    A multichaperone nucleosome-remodeling complex that contains the H1 linker histone chaperone nuclear autoantigenic sperm protein (NASP) has recently been described...
  3. Wang H, Walsh S, Parthun M. Expanded binding specificity of the human histone chaperone NASP. Nucleic Acids Res. 2008;36:5763-72 pubmed publisher
    NASP (nuclear autoantigenic sperm protein) has been reported to be an H1-specific histone chaperone...
  4. Alekseev O, Richardson R, TSURUTA J, O Rand M. Depletion of the histone chaperone tNASP inhibits proliferation and induces apoptosis in prostate cancer PC-3 cells. Reprod Biol Endocrinol. 2011;9:50 pubmed publisher
    NASP (Nuclear Autoantigenic Sperm Protein) is a histone chaperone that is present in all dividing cells. NASP has two splice variants: tNASP and sNASP...
  5. Alekseev O, Richardson R, Alekseev O, O Rand M. Analysis of gene expression profiles in HeLa cells in response to overexpression or siRNA-mediated depletion of NASP. Reprod Biol Endocrinol. 2009;7:45 pubmed publisher
    NASP (Nuclear Autoantigenic Sperm Protein) is a linker histone chaperone required for normal cell division. Changes in NASP expression significantly affect cell growth and development; loss of gene function results in embryonic lethality...
  6. Drané P, Ouararhni K, Depaux A, Shuaib M, Hamiche A. The death-associated protein DAXX is a novel histone chaperone involved in the replication-independent deposition of H3.3. Genes Dev. 2010;24:1253-65 pubmed publisher
    ..Taken together, our data demonstrate that DAXX functions as a bona fide histone chaperone involved in the replication-independent deposition of H3.3. ..
  7. Osakabe A, Tachiwana H, Matsunaga T, Shiga T, Nozawa R, Obuse C, et al. Nucleosome formation activity of human somatic nuclear autoantigenic sperm protein (sNASP). J Biol Chem. 2010;285:11913-21 pubmed publisher
    NASP (nuclear autoantigenic sperm protein) is a member of the N1/N2 family, which is widely conserved among eukaryotes. Human NASP reportedly prefers to bind to histones H3.H4 and the linker histone H1, as compared with H2A...
  8. Ask K, Jasencakova Z, Menard P, Feng Y, Almouzni G, Groth A. Codanin-1, mutated in the anaemic disease CDAI, regulates Asf1 function in S-phase histone supply. EMBO J. 2012;31:2013-23 pubmed publisher
    ..This function is compromised by two CDAI mutations that impair complex formation with Asf1, providing insight into the molecular basis for CDAI disease. ..
  9. Welch J, O Rand M. Characterization of a sperm-specific nuclear autoantigenic protein. II. Expression and localization in the testis. Biol Reprod. 1990;43:569-78 pubmed
    The testis- and sperm-specific nuclear autoantigenic protein, NASP, has a 2.5-kb mRNA that encodes a protein of molecular weight 73,533 and has several structural features of nuclear proteins...
  10. Ma W, Xie S, Ni M, Huang X, Hu S, Liu Q, et al. MicroRNA-29a inhibited epididymal epithelial cell proliferation by targeting nuclear autoantigenic sperm protein (NASP). J Biol Chem. 2012;287:10189-99 pubmed publisher
    ..The nuclear autoantigenic sperm protein (NASP), a novel target of miR-29a, was significantly down-regulated during postnatal development of ..
  11. Wang H, Ge Z, Walsh S, Parthun M. The human histone chaperone sNASP interacts with linker and core histones through distinct mechanisms. Nucleic Acids Res. 2012;40:660-9 pubmed publisher
    Somatic nuclear autoantigenic sperm protein (sNASP) is a human homolog of the N1/N2 family of histone chaperones...
  12. Li J, Wei C, Cao S, Xia M. Elevated nuclear auto-antigenic sperm protein promotes melanoma progression by inducing cell proliferation. Onco Targets Ther. 2019;12:2105-2113 pubmed publisher
    Nuclear auto-antigenic sperm protein (NASP) has been implicated in tumorigenesis. However, its role in melanoma is still unclear. In the present study, we detected the mRNA and protein level of NASP in melanoma cell lines and tissues...
  13. Wei D, Zhang Y, Liu Y, Li W, Chen Z, Smagghe G, et al. Gene expression profiling of ovary identified eggshell proteins regulated by 20-hydroxyecdysone in Bactrocera dorsalis. Comp Biochem Physiol Part D Genomics Proteomics. 2019;30:206-216 pubmed publisher
    ..potential functions in ovarian development were also identified, including vitellogenin receptor, insulin receptor, NASP protein, and odorant binding protein...
  14. Apta Smith M, Hernández Fernaud J, Bowman A. Evidence for the nuclear import of histones H3.1 and H4 as monomers. EMBO J. 2018;37: pubmed publisher
    ..tethered histones are bound to importin-alpha proteins (predominantly IPO4), but not to histone-specific chaperones NASP, ASF1a, RbAp46 (RBBP7) or HAT1, which reside in the nucleus in interphase cells...
  15. Tripathi A, Singh K, Pareek A, Singla Pareek S. Histone chaperones in Arabidopsis and rice: genome-wide identification, phylogeny, architecture and transcriptional regulation. BMC Plant Biol. 2015;15:42 pubmed publisher
    ..These could be classified into seven different families, namely NAP, CAF1, SPT6, ASF1, HIRA, NASP, and FACT...
  16. Serra Sogas N, O Hara P, Canessa R. Modeling the distribution of illicit oily discharges detected by aerial surveillance in western Canadian marine waters. Mar Pollut Bull. 2014;87:76-87 pubmed publisher
    ..from vessel operations have been documented in Canada's Pacific region by the National Aerial Surveillance Program (NASP) since the early 1990s...
  17. Cloete L, Tanov E, Muhire B, Martin D, Harkins G. The influence of secondary structure, selection and recombination on rubella virus nucleotide substitution rate estimates. Virol J. 2014;11:166 pubmed publisher the PARRIS and FUBAR methods), at nucleotide sites within the genomic secondary structures (predicted by the NASP method). We determine that RV nucleotide substitution rates range from 1...
  18. Kang X, Feng Y, Gan Z, Zeng S, Guo X, Chen X, et al. NASP antagonize chromatin accessibility through maintaining histone H3K9me1 in hepatocellular carcinoma. Biochim Biophys Acta Mol Basis Dis. 2018;1864:3438-3448 pubmed publisher
    ..Here, we studied the function of nuclear autoantigenic sperm protein (NASP) in the regulation of liver cancer...
  19. Dannah N, Nabeel Shah S, Kurat C, Sabatinos S, Fillingham J. Functional Analysis of Hif1 Histone Chaperone in Saccharomyces cerevisiae. G3 (Bethesda). 2018;8:1993-2006 pubmed publisher
    ..Hif1, as well as its human homolog NASP, has been implicated in an array of chromatin-related processes including histone H3/H4 transport, chromatin ..
  20. Yang F, Zuo Y, Zhou W, Xia C, Hahm B, Sullivan M, et al. sNASP inhibits TLR signaling to regulate immune response in sepsis. J Clin Invest. 2018;128:2459-2472 pubmed publisher
    ..Here, we show that somatic nuclear autoantigenic sperm protein (sNASP) binds to TRAF6 to prevent TRAF6 autoubiquitination in unstimulated macrophages...
  21. Embogama D, Pflum M. K-BILDS: A Kinase Substrate Discovery Tool. Chembiochem. 2017;18:136-141 pubmed publisher
    ..Of the candidate substrates, nuclear autoantigenic sperm protein (NASP), BCL2-associated athanogene?3 (BAG3), and 14-3-3 protein Tau (YWHAQ) were validated as novel ..
  22. Sadeesh E, Fozia S, Meena K. Combined positive effect of oocyte extracts and brilliant cresyl blue stained recipient cytoplasts on epigenetic reprogramming and gene expression in buffalo nuclear transfer embryos. Cytotechnology. 2017;69:289-305 pubmed publisher
    ..This ETCs dedifferentiated into neuron-like lineage under appropriate induction condition. The RA of NASP, EEF1A1, DNMT1, ODC1 and RPS27A was increased (P < 0...
  23. Zhou W, Luo Z, Tang C, Zou X, Zhao L, Fang P. Is there an improvement of antibiotic use in China? Evidence from the usage analysis of combination antibiotic therapy for type I incisions in 244 hospitals. J Huazhong Univ Sci Technolog Med Sci. 2016;36:772-779 pubmed publisher
    ..Data were collected with regards to the implementation of the national antibiotic stewardship program (NASP), the overall usage and the prophylactic use of antibiotic for type I incisions...
  24. Nagatomo H, Kohri N, Akizawa H, Hoshino Y, Yamauchi N, Kono T, et al. Requirement for nuclear autoantigenic sperm protein mRNA expression in bovine preimplantation development. Anim Sci J. 2016;87:457-61 pubmed publisher
    b>Nuclear autoantigenic sperm protein (NASP) is associated with DNA replication, cell proliferation, and cell cycle progression through its specific binding to histones...
  25. Haigney A, Ricketts M, Marmorstein R. Dissecting the Molecular Roles of Histone Chaperones in Histone Acetylation by Type B Histone Acetyltransferases (HAT-B). J Biol Chem. 2015;290:30648-57 pubmed publisher
    ..of a trimeric NuB4 complex containing Hat1, Hat2, and a histone H3-H4 specific histone chaperone called Hif1 (NASP)...
  26. González Hernández A, Van Coppernolle S, Borloo J, Van Meulder F, Paerewijck O, Peelaers I, et al. Host protective ASP-based vaccine against the parasitic nematode Ostertagia ostertagi triggers NK cell activation and mixed IgG1-IgG2 response. Sci Rep. 2016;6:29496 pubmed publisher
    ..capacity of a vaccine against this parasite based on a native activation-associated secreted protein ASP1 (nASP) in combination with the saponin adjuvant QuilA...
  27. Duschek L, Beyer A, Oelerich J, Volz K. Composition determination of multinary III/V semiconductors via STEM HAADF multislice simulations. Ultramicroscopy. 2018;185:15-20 pubmed publisher
    ..Here, we describe the proposed method in detail using Ga(NAsP) as an example multinary material.
  28. Yu B, Chen X, Li J, Gu Q, Zhu Z, Li C, et al. microRNA-29c inhibits cell proliferation by targeting NASP in human gastric cancer. BMC Cancer. 2017;17:109 pubmed publisher
    ..We additionally show that miR-29c exerts these effects by targeting Nuclear autoantigenic sperm protein (NASP). Moreover, depletion of NASP can elite the phenotypes caused by miR-29c...
  29. Batova I, Richardson R, Widgren E, O Rand M. Analysis of the autoimmune epitopes on human testicular NASP using recombinant and synthetic peptides. Clin Exp Immunol. 2000;121:201-9 pubmed
    The human nuclear autoantigenic sperm protein, NASP, is a testicular histone-binding protein of 787 amino acids to which most vasectomized men develop autoantibodies...
  30. Zhang M, Liu H, Gao Y, Zhu Z, Chen Z, Zheng P, et al. Structural Insights into the Association of Hif1 with Histones H2A-H2B Dimer and H3-H4 Tetramer. Structure. 2016;24:1810-1820 pubmed publisher
    ..These specificities are conserved features of the Sim3-Hif1-NASP interrupted tetratricopeptide repeat proteins, which provide clues for investigating their potential roles in ..
  31. Bowman A, Lercher L, Singh H, Zinne D, Timinszky G, Carlomagno T, et al. The histone chaperone sNASP binds a conserved peptide motif within the globular core of histone H3 through its TPR repeats. Nucleic Acids Res. 2016;44:3105-17 pubmed publisher
  32. Fang J, Wang H, Xi W, Cheng G, Wang S, Su S, et al. Downregulation of tNASP inhibits proliferation through regulating cell cycle-related proteins and inactive ERK/MAPK signal pathway in renal cell carcinoma cells. Tumour Biol. 2015;36:5209-14 pubmed publisher
    Nuclear auto-antigenic sperm protein (NASP), initially described as a highly auto-immunogenic testis and sperm-specific protein, is a histone chaperone that is proved to present in all dividing cells...
  33. Kato D, Osakabe A, Tachiwana H, Tanaka H, Kurumizaka H. Human tNASP promotes in vitro nucleosome assembly with histone H3.3. Biochemistry. 2015;54:1171-9 pubmed publisher
    ..1-H4 complex. These results provide evidence that tNASP is ubiquitously produced in various types of human tissues and promotes in vitro nucleosome assembly with H3 variant specificity. ..
  34. Richardson R, Bencic D, O Rand M. Comparison of mouse and human NASP genes and expression in human transformed and tumor cell lines. Gene. 2001;274:67-75 pubmed
    We previously cloned and sequenced cDNAs encoding mouse NASP (mNASP), a cell cycle regulated histone H1 binding protein. Here we report the genomic sequence and organization for mNASP along with its 5' regulatory region and compare these ..
  35. Silva A, Chen J, Carraro D, Wang S, Camargo A. Generation of longer 3' cDNA fragments from massively parallel signature sequencing tags. Nucleic Acids Res. 2004;32:e94 pubmed
    ..A systematic large-scale analysis of the genome by MPSS, in combination with the use of GLGI-MPSS protocol, will certainly provide a complementary approach to generate the complete catalog of human transcripts. ..
  36. Foltz D, Jansen L, Bailey A, Yates J, Bassett E, Wood S, et al. Centromere-specific assembly of CENP-a nucleosomes is mediated by HJURP. Cell. 2009;137:472-84 pubmed publisher
    ..We propose HJURP to be a cell-cycle-regulated CENP-A-specific histone chaperone required for centromeric chromatin assembly. ..
  37. Ding C, Sun H, Ren J, Qu X. Immobilization of enzyme on chiral polyelectrolyte surface. Anal Chim Acta. 2017;952:88-95 pubmed publisher
    Chiral D- and L-N-acryloyl aspartic acid (NAsp) polyelectrolyte (PE) surfaces with similar chemical compositions and physical properties but opposite chirality are designed for enzyme immobilization...
  38. Nabeel Shah S, Ashraf K, Pearlman R, Fillingham J. Molecular evolution of NASP and conserved histone H3/H4 transport pathway. BMC Evol Biol. 2014;14:139 pubmed publisher
    b>NASP is an essential protein in mammals that functions in histone transport pathways and maintenance of a soluble reservoir of histones H3/H4...
  39. Minami N, Sasaki K, Aizawa A, Miyamoto M, Imai H. Analysis of gene expression in mouse 2-cell embryos using fluorescein differential display: comparison of culture environments. Biol Reprod. 2001;64:30-5 pubmed
    ..that the genes for ATP synthase (ATPase 6), S:-adenosylmethionine decarboxylase (S:-AMDC) and nuclear autoantigenic sperm protein (NASP) were differentially expressed in embryos cultured in the oviductal environment (nonblocking ..
  40. Lee Y, O Rand M. Ultrastructural localization of a nuclear autoantigenic sperm protein in spermatogenic cells and spermatozoa. Anat Rec. 1993;236:442-8 pubmed
    This study demonstrates the ultrastructural localization of rabbit nuclear autoantigenic sperm protein (NASP) in spermatogenic cells and spermatozoa. NASP is present in rabbits, rats, mice, and human testes and spermatozoa...
  41. Groth A, Ray Gallet D, Quivy J, Lukas J, Bartek J, Almouzni G. Human Asf1 regulates the flow of S phase histones during replicational stress. Mol Cell. 2005;17:301-11 pubmed
  42. Loyola A, Tagami H, Bonaldi T, Roche D, Quivy J, Imhof A, et al. The HP1alpha-CAF1-SetDB1-containing complex provides H3K9me1 for Suv39-mediated K9me3 in pericentric heterochromatin. EMBO Rep. 2009;10:769-75 pubmed publisher