MYL4

Summary

Gene Symbol: MYL4
Description: myosin, light chain 4, alkali; atrial, embryonic
Alias: ALC1, AMLC, GT1, myosin light chain 1, embryonic muscle/atrial isoform, myosin light chain 4, myosin light chain alkali GT-1 isoform, myosin, atrial/fetal muscle, light chain, myosin, light polypeptide 4, alkali; atrial, embryonic
Species: human

Top Publications

  1. ncbi Characterization of gonadotropin-releasing hormone gene transcripts in a mouse hypothalamic neuronal GT1 cell line
    T T Yeo
    Fishberg Research Center for Neurobiology, Mount Sinai School of Medicine, New York, NY 10029, USA
    Brain Res Mol Brain Res 42:255-62. 1996
  2. ncbi Functional characterization of the human atrial essential myosin light chain (hALC-1) in a transgenic rat model
    Ahmed Ihab Abdelaziz
    Max Delbrück Center for Molecular Medicine MDC, Molecular Muscle Physiology, Robert Rossle Strasse 10, 13125 Berlin, Germany
    J Mol Med (Berl) 82:265-74. 2004
  3. ncbi Minigenes encoding N-terminal domains of human cardiac myosin light chain-1 improve heart function of transgenic rats
    Hannelore Haase
    Max Delbruck Center for Molecular Medicine, Berlin, Germany
    FASEB J 20:865-73. 2006
  4. ncbi Proteomic analysis of pharmacologically preconditioned cardiomyocytes reveals novel phosphorylation of myosin light chain 1
    D K Arrell
    Department of Physiology, Queen s University, Kingston, Ontario, Canada
    Circ Res 89:480-7. 2001
  5. ncbi Human atrial myosin light chain 1 expression attenuates heart failure
    Ahmed Ihab Abdelaziz
    Max Delbruck Center for Molecular Medicine, Robert Rossle Str 10, 13125 Berlin, Germany
    Adv Exp Med Biol 565:283-92; discussion 92, 405-15. 2005
  6. ncbi Transcription factors Oct-1 and C/EBPbeta (CCAAT/enhancer-binding protein-beta) are involved in the glutamate/nitric oxide/cyclic-guanosine 5'-monophosphate-mediated repression of mediated repression of gonadotropin-releasing hormone gene expression
    D D Belsham
    Department of Reproductive Medicine, The Center for Molecular Genetics, University of Califonia, La Jolla 92093, USA
    Mol Endocrinol 14:212-28. 2000
  7. ncbi Transcriptional regulation of P450scc gene expression in neural and steroidogenic cells: implications for regulation of neurosteroidogenesis
    P Zhang
    Department of Obstetrics, Gynecology, and Reproductive Sciences, University of California, San Francisco 94143, USA
    Mol Endocrinol 9:1571-82. 1995
  8. ncbi Modulation of contractility in human cardiac hypertrophy by myosin essential light chain isoforms
    M C Schaub
    Institute of Pharmacology, University of Zurich, Switzerland
    Cardiovasc Res 37:381-404. 1998
  9. pmc Differences between two real-time PCR-based hepatitis C virus (HCV) assays (RealTime HCV and Cobas AmpliPrep/Cobas TaqMan) and one signal amplification assay (Versant HCV RNA 3.0) for RNA detection and quantification
    Johannes Vermehren
    Zentrum der Inneren Medizin, Medizinische Klinik 1, Klinikum der JW Goethe Universität, Frankfurt am Main, Germany
    J Clin Microbiol 46:3880-91. 2008
  10. ncbi A novel mechanism for endocrine-disrupting effects of polychlorinated biphenyls: direct effects on gonadotropin-releasing hormone neurones
    A C Gore
    Kastor Neurobiology of Aging Laboratories, Fishberg Research Center for Neurobiology, and Brookdale Department of Geriatrics and Adult Development, Mount Sinai School of Medicine, New York, NY 10029, USA
    J Neuroendocrinol 14:814-23. 2002

Research Grants

  1. NEUROSECRETORY GENE EXPRESSION IN THE HYPOTHALAMUS
    PAMELA MELLON; Fiscal Year: 1993
  2. NEUROSECRETORY GENE EXPRESSION IN THE HYPOTHALAMUS
    Pamela L Mellon; Fiscal Year: 2011
  3. NEUROSECRETORY GENE EXPRESSION IN THE HYPOTHALAMUS
    PAMELA MELLON; Fiscal Year: 2007
  4. Circadian Regulation of Gonadotropin-Releasing Hormone
    Patrick E Chappell; Fiscal Year: 2010
  5. B-Synuclein as a Treatment for Lewy Body Disease
    Eliezer Masliah; Fiscal Year: 2005
  6. MOLECULAR MECHANISM OF PHOTORECEPTOR G PROTEIN SIGNALING
    Nikolai O Artemyev; Fiscal Year: 2010
  7. Functional Characterization of Leishmania Transporters
    Scott Landfear; Fiscal Year: 2007
  8. CELLULAR PROTEIN MATURATION AND DEGRADATION
    Daniel Hebert; Fiscal Year: 2009
  9. Functional Characterization of Leishmania Transporters
    Scott M Landfear; Fiscal Year: 2010
  10. CELLULAR PROTEIN MATURATION AND DEGRADATION
    Daniel N Hebert; Fiscal Year: 2010

Detail Information

Publications148 found, 100 shown here

  1. ncbi Characterization of gonadotropin-releasing hormone gene transcripts in a mouse hypothalamic neuronal GT1 cell line
    T T Yeo
    Fishberg Research Center for Neurobiology, Mount Sinai School of Medicine, New York, NY 10029, USA
    Brain Res Mol Brain Res 42:255-62. 1996
    ..RNA transcripts derived from the gonadotropin releasing hormone (GnRH) gene in a mouse hypothalamic neuronal GT1 cell line...
  2. ncbi Functional characterization of the human atrial essential myosin light chain (hALC-1) in a transgenic rat model
    Ahmed Ihab Abdelaziz
    Max Delbrück Center for Molecular Medicine MDC, Molecular Muscle Physiology, Robert Rossle Strasse 10, 13125 Berlin, Germany
    J Mol Med (Berl) 82:265-74. 2004
    ..2 to 1,364 +/- 137.4 mmHg/s in the relaxation rate in the WKY and the TGR groups respectively. Characterizing the functional effects of hALC-1 at the whole organ level represents a step towards gene therapy of heart failure...
  3. ncbi Minigenes encoding N-terminal domains of human cardiac myosin light chain-1 improve heart function of transgenic rats
    Hannelore Haase
    Max Delbruck Center for Molecular Medicine, Berlin, Germany
    FASEB J 20:865-73. 2006
    ..The positive inotropic effect of MLC-1 peptides occurred in the absence of a hypertrophic response. Thus, expression of N-terminal domains of MLC-1 represent a valuable tool for the treatment of the failing heart...
  4. ncbi Proteomic analysis of pharmacologically preconditioned cardiomyocytes reveals novel phosphorylation of myosin light chain 1
    D K Arrell
    Department of Physiology, Queen s University, Kingston, Ontario, Canada
    Circ Res 89:480-7. 2001
    ..This proteomic analysis of preconditioned myocardium has revealed a previously unsuspected in vivo posttranslational modification to MLC1...
  5. ncbi Human atrial myosin light chain 1 expression attenuates heart failure
    Ahmed Ihab Abdelaziz
    Max Delbruck Center for Molecular Medicine, Robert Rossle Str 10, 13125 Berlin, Germany
    Adv Exp Med Biol 565:283-92; discussion 92, 405-15. 2005
    ..These results demonstrate that the expression of hALC-1 could have a beneficial effect on the overloaded hypertrophied heart...
  6. ncbi Transcription factors Oct-1 and C/EBPbeta (CCAAT/enhancer-binding protein-beta) are involved in the glutamate/nitric oxide/cyclic-guanosine 5'-monophosphate-mediated repression of mediated repression of gonadotropin-releasing hormone gene expression
    D D Belsham
    Department of Reproductive Medicine, The Center for Molecular Genetics, University of Califonia, La Jolla 92093, USA
    Mol Endocrinol 14:212-28. 2000
    ..transduction pathway through cGMP-dependent protein kinase in the hypothalamic GnRH-secreting neuronal cell line GT1-7. This repression localized within a previously characterized 300-bp neuron-specific enhancer...
  7. ncbi Transcriptional regulation of P450scc gene expression in neural and steroidogenic cells: implications for regulation of neurosteroidogenesis
    P Zhang
    Department of Obstetrics, Gynecology, and Reproductive Sciences, University of California, San Francisco 94143, USA
    Mol Endocrinol 9:1571-82. 1995
    ..gene was assessed in mouse adrenocortical Y-1, mouse Leydig MA-10, rat C6 glioma, rat GC somatotrope, and mouse GT1-7 neurosecretory cell lines...
  8. ncbi Modulation of contractility in human cardiac hypertrophy by myosin essential light chain isoforms
    M C Schaub
    Institute of Pharmacology, University of Zurich, Switzerland
    Cardiovasc Res 37:381-404. 1998
    ..beta-myosin heavy chains remain unchanged, but the ectopic expression of the atrial myosin essential light chain (ALC1) partially replaces the endogenous ventricular isoform (VLC1)...
  9. pmc Differences between two real-time PCR-based hepatitis C virus (HCV) assays (RealTime HCV and Cobas AmpliPrep/Cobas TaqMan) and one signal amplification assay (Versant HCV RNA 3.0) for RNA detection and quantification
    Johannes Vermehren
    Zentrum der Inneren Medizin, Medizinische Klinik 1, Klinikum der JW Goethe Universität, Frankfurt am Main, Germany
    J Clin Microbiol 46:3880-91. 2008
    ..Commercially available HCV RNA assays are calibrated to the HCV genotype 1 (gt1)-based WHO standard. Significant differences between assays have been reported...
  10. ncbi A novel mechanism for endocrine-disrupting effects of polychlorinated biphenyls: direct effects on gonadotropin-releasing hormone neurones
    A C Gore
    Kastor Neurobiology of Aging Laboratories, Fishberg Research Center for Neurobiology, and Brookdale Department of Geriatrics and Adult Development, Mount Sinai School of Medicine, New York, NY 10029, USA
    J Neuroendocrinol 14:814-23. 2002
    ..The effects of two PCB mixtures, Aroclor 1221 and Aroclor 1254, were tested on the hypothalamic GT1-7 cells, which synthesize and secrete the key hypothalamic hormone, gonadotropin-releasing hormone (GnRH)...
  11. ncbi Specific gene targeting in Spiroplasma citri: improved vectors and production of unmarked mutations using site-specific recombination
    Sybille Duret
    UMR 1090 Génomique Développement et Pouvoir Pathogène, INRA, Universite de Bordeaux 2, Centre INRA de Bordeaux, Villenave D Ornon, France
    Microbiology 151:2793-803. 2005
    ..In contrast to the wild-type strain GII-3, the crr-disrupted mutant GII3-gt1 used neither glucose nor trehalose, indicating that in S...
  12. pmc A virus-induced gene silencing screen identifies a role for Thylakoid Formation1 in Pseudomonas syringae pv tomato symptom development in tomato and Arabidopsis
    Tamding Wangdi
    Plant Biology Division, Samuel Roberts Noble Foundation, Ardmore, Oklahoma 73401, USA
    Plant Physiol 152:281-92. 2010
    ..These were designated as ALC (for altered COR response) genes. When silenced, one of the identified genes, ALC1, produced a hypersensitive/necrosis-like phenotype upon COR application in a Coronatine-Insensitive1 (COI1)-..
  13. ncbi Neuropeptide-induced transactivation of a neuronal epidermal growth factor receptor is mediated by metalloprotease-dependent formation of heparin-binding epidermal growth factor
    Bukhtiar H Shah
    Endocrinology and Reproduction Research Branch, NICHD, National Institutes of Health, Bethesda, Maryland 20892 4510, USA
    J Biol Chem 279:414-20. 2004
    ..In immortalized hypothalamic neurons (GT1-7 cells), agonist binding to the gonadotropin-releasing hormone receptor (GnRH-R) causes phosphorylation of MAPKs ..
  14. ncbi Effects of glucose and insulin on glucokinase activity in rat hypothalamus
    Carmen Sanz
    Department of Biochemistry and Molecular Biology, Faculty of Medicine, Complutense University, Madrid, Spain
    J Endocrinol 193:259-67. 2007
    ..and on the activity of this enzyme in the hypothalamus, we used two kinds of biological models: hypothalamic GT1-7 cells and rat hypothalamic slices...
  15. ncbi Transforming growth factor beta1 may directly influence gonadotropin-releasing hormone gene expression in the rat hypothalamus
    Sebastien Bouret
    Institut National de la Santé et de la Recherche Médicale Unité 422, Lille, France
    Endocrinology 145:1794-801. 2004
    In vitro studies using immortalized GT1 cells suggest that hypothalamic astrocytes employ TGFbeta(1) to directly regulate the secretion of GnRH, the neurohormone that controls sexual maturation and adult reproductive function...
  16. pmc Potentiation, activation and blockade of GABAA receptors of clonal murine hypothalamic GT1-7 neurones by propofol
    S Adodra
    Department of Anesthesiology, UCLA Medical Center 90024, USA
    Br J Pharmacol 115:953-60. 1995
    ..and the intravenous general anaesthetic propofol (2,6-diisopropylphenol) on GABAA receptors of self-replicating GT1-7 hypothalamic neurones were investigated by the patch clamp technique. 2...
  17. ncbi Substrate specificity and inhibitor studies of a membrane-bound ganglioside sialidase isolated from human brain tissue
    Christian Oehler
    Institute of Pathochemistry and Neurochemistry, University of Heidelberg, Im Neuenheimer Feld 220 221, D 69120 Heidelberg, Germany
    Biol Chem 383:1735-42. 2002
    ..The sialidase removed terminal sialic acids from gangliosides GM3, GM4, GD3, GD2, GD1 a, GD1 b, GT1 b and GQ1 b, but was inactive toward gangliosides with sialic acid in a branching position (as in GM1 and GM2)...
  18. ncbi Promoter usage and estrogen regulation of prolactin receptor gene in the brain of the female rat
    Xiujun Pi
    Department of Molecular and Integrative Physiology, Kansas University Medical Center, Kansas City, Kansas 66160 7401, USA
    Neuroendocrinology 77:187-97. 2003
    ..The expression of PRL-R mRNA was up-regulated by estrogen treatment in the rat brain tissue and in the GT1-7 cell culture...
  19. ncbi Characterization of chloride efflux from GT1-7 neurons: lack of effect of ethanol on GABAA response
    M A Javors
    Department of Psychiatry, University of Texas Health Science Center, San Antonio 78284, USA
    Brain Res 780:183-9. 1998
    The purpose of this study of GT1-7 neurons was to partially characterize basal Cl- transport and GABAA mediated Cl- efflux and to test the effect of ethanol on a GABAA receptor that lacks a gamma subunit...
  20. ncbi Physical and linkage mapping of human chromosome 17 loci to dog chromosomes 9 and 5
    P Werner
    Center for Comparative Medical Genetics, School of Veterinary Medicine, University of Pennsylvania, Philadelphia 19104 6010, USA
    Genomics 42:74-82. 1997
    ..centromeric two-thirds of dog chromosome 9 (CFA9), an acrocentric chromosome of medium size: P4HB, GALK1, TK1, GH1, MYL4, BRCA1, RARA, THRA1, MPO, NF1, and CRYBA1. Eight of these were also positioned on a linkage map spanning 38.6 cM...
  21. ncbi Structure-function analysis of the UDP-N-acetyl-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase. Essential residues lie in a predicted active site cleft resembling a lactose repressor fold
    F K Hagen
    Center for Oral Biology, Rochester Institute of Biomedical Sciences, University of Rochester, Rochester, New York 14642, USA
    J Biol Chem 274:6797-803. 1999
    ..The 112-amino acid glycosyltransferase 1 (GT1) motif represents the first half of the catalytic unit and contains a short aspartate-any residue-histidine (DXH) ..
  22. pmc A glutamine transport gene, glnQ, is required for fibronectin adherence and virulence of group B streptococci
    Glen S Tamura
    Children s Hospital and Regional Medical Center and the University of Washington, Seattle, Washington 98105, USA
    Infect Immun 70:2877-85. 2002
    ..GBS adhere to fibronectin when it is attached to a solid phase. We isolated a Tn917 transposon mutant, COH1-GT1, which shows decreased adherence to fibronectin...
  23. pmc Structure of the human gene and two rat cDNAs encoding the alpha chain of GTP-binding regulatory protein Go: two different mRNAs are generated by alternative splicing
    T Tsukamoto
    Institute of Medical Science, University of Tokyo, Japan
    Proc Natl Acad Sci U S A 88:2974-8. 1991
    ..Matsuoka, M. & Kaziro, Y. (1988) J. Biol. Chem. 263, 6656-6664] and also transducin G protein alpha subunit Gt1 alpha [Raport, C. J., Dere, B. & Hurley, J. (1989) J. Biol. Chem. 264, 7122-7128]...
  24. ncbi Concentration-dependent effects of muscimol to enhance pulsatile GnRH release from GT1-7 neurons in vitro
    T S King
    Department of Cellular and Structural Biology, The University of Texas Health Science Center, San Antonio, TX 78284 7762, USA
    Brain Res 824:56-62. 1999
    Immortalized GT1-7 neurons were used to characterize the effect of muscimol, a GABAA receptor agonist, to enhance pulsatile gonadotropin-releasing hormone (GnRH) release...
  25. ncbi Scavenging of hydrogen peroxide and inhibition of ultraviolet light-induced oxidative DNA damage by aqueous extracts from green and black teas
    H Wei
    Department of Dermatology, Mount Sinai NYU Medical Center, New York, NY 10029, USA
    Free Radic Biol Med 26:1427-35. 1999
    ..been extracted by serial chloroform, ethyl acetate and n-butanol, and divided into four subfractions designated as GT1-4 for green tea and BT1-4 for black tea, respectively...
  26. ncbi Cross-talk signals in the CNS: role of neurotrophic and hormonal factors, adhesion molecules and intercellular signaling agents in luteinizing hormone-releasing hormone (LHRH)-astroglial interactive network
    B Marchetti
    Department of Pharmacology, Medical School, University of Catania, 95125 Catania, Italy
    Front Biosci 2:d88-125. 1997
    ..Different experimental paradigms such as co-culture and mixed culture models between the GT1-1 neurons and astroglial cells in primary culture, disclosed the presence of a bi-directional flow of informational ..
  27. ncbi Second messenger regulation of mouse gonadotropin-releasing hormone gene expression in immortalized mouse hypothalamic GT1-3 cells
    K L Yu
    Dr Arthur M Fishberg Research Center for Neurobiology, Mount Sinai School of Medicine, New York, NY 10029
    Mol Cell Endocrinol 102:85-92. 1994
    Using a transgenic mouse derived GnRH expressing neuronal cell line, GT1-3, we studied the effects of activation of cAMP, Ca2+ and protein kinase C pathways by forskolin, ionomycin and the phorbol ester phorbol 12-myristate 13-acetate (..
  28. doi Cetrorelix, a gonadotropin-releasing hormone antagonist, induces the expression of melatonin receptor 1a in the gonadotropin-releasing hormone neuronal cell line GT1-7
    Hirotaka Ishii
    Department of Physiology, Nippon Medical School, Tokyo, Japan
    Neuroendocrinology 90:251-9. 2009
    ..Using immortalized GnRH neurons (GT1-7 cells), the present study investigated the mechanism by which the expression of melatonin receptors is regulated ..
  29. ncbi Connexin 26 expression and extensive gap junctional coupling in cultures of GT1-7 cells secreting gonadotropin-releasing hormone
    L Hu
    Department of Obstetrics and Gynecology, Reproductive Endocrinology Center, San Francisco, Calif, USA
    Neuroendocrinology 70:221-7. 1999
    ..A previous study using GnRH-secreting GT1-7 cells reported that connexin 26 was the major GJ subunit present, and that about 20% of the cultured cells ..
  30. ncbi [An experimental study of the role of myosin light chain in myogenesis in vitro]
    Suzhen Zhang
    School of Life Science, Sichuan University, Chengdu Sichuan, 610041, P R China
    Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi 22:753-8. 2008
    ..To investigate the role of myosin light chain (Myl) in myogenesis in vitro...
  31. doi Contrasting strategies to cope with chilling stress among clones of a tropical tree, Hevea brasiliensis
    Jing Mai
    Clermont Universite, Universite Blaise Pascal, UMR PIAF, BP 10448, F 63000 Clermont Ferrand Cedex, France
    Tree Physiol 30:1391-402. 2010
    Eight Hevea brasiliensis Muell. Arg. clones (GT1, YUNYAN77-4, IRCA707, IRCA317, PB217, PB260, PR107 and RRIM600) were compared for their tolerance towards chilling stress...
  32. doi Precise cell patterning using cytophobic self-assembled monolayer deposited on top of semi-transparent gold
    Gaoshan Jing
    Sherman Fairchild Center, Department of Electrical and Computer Engineering, Lehigh University, Bethlehem, USA
    Biomed Microdevices 12:935-48. 2010
    ..the patterning of four different kinds of cells using this technique: immortalized mouse hypothalamic neurons (GT1-7), mouse osteoblast cells (MC3T3), mouse fibroblast cells (NIH3T3) and primary rat hippocampal neurons...
  33. pmc Linkage analysis and comparative mapping of canine progressive rod-cone degeneration (prcd) establishes potential locus homology with retinitis pigmentosa (RP17) in humans
    G M Acland
    James A Baker Institute for Animal Health, College of Veterinary Medicine, Cornell University, Ithaca NY 14853, USA
    Proc Natl Acad Sci U S A 95:3048-53. 1998
    ..Identification of a linkage group flanking prcd ([TK1, GALK1, prcd]-[MYL4, C09.173, C09.2263]-RARA-C09.250-C09...
  34. doi Depolarizing shift in the GABA-induced current reversal potential by lidocaine hydrochloride
    Yoshihisa Nakahata
    Department of Developmental Physiology, National Institute for Physiological Sciences, Okazaki, Japan
    Brain Res 1345:19-27. 2010
    ..Furthermore, LC-HCl caused a depolarizing shift of E(GABA) in cultured GT1-7 cells expressing KCC2 but failed to change E(GABA) in GT1-7 cells without expression of KCC2...
  35. ncbi Neuroprotective effects of nicergoline in immortalized neurons
    M A Sortino
    Institute of Pharmacology, University of Catania School of Medicine, Italy
    Eur J Pharmacol 368:285-90. 1999
    We studied the potential neuroprotective action of nicergoline in immortalized hypothalamic GT1-7 cells exposed to agents which deplete levels of reduced glutathione, thus causing oxidative stress and cell death...
  36. pmc Regulation of GNRH production by estrogen and bone morphogenetic proteins in GT1-7 hypothalamic cells
    Hiroyuki Otani
    Department of Medicine and Clinical Science, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, 2 5 1 Shikata cho, Kitaku, Okayama City 700 8558, Japan
    J Endocrinol 203:87-97. 2009
    ..We here investigated the effects of BMPs on GNRH production controlled by estrogen using murine GT1-7 hypothalamic neuron cells...
  37. ncbi Roles of Src and epidermal growth factor receptor transactivation in transient and sustained ERK1/2 responses to gonadotropin-releasing hormone receptor activation
    Bukhtiar H Shah
    Endocrinology and Reproduction Research Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892 4510, USA
    J Biol Chem 278:19118-26. 2003
    ..is relatively sustained in alpha T3-1 pituitary gonadotropes and HEK293 cells but is transient in immortalized GT1-7 neurons...
  38. ncbi A contactin-receptor-like protein tyrosine phosphatase beta complex mediates adhesive communication between astroglial cells and gonadotrophin-releasing hormone neurones
    A S Parent
    Division of Neuroscience, Oregon National Primate Research Center, Beaverton, OR 97006, USA
    J Neuroendocrinol 19:847-59. 2007
    ..b>GT1-7 immortalised GnRH neurones adhere to the CAH domain of RPTPbeta, and this adhesiveness is blocked when contactin ..
  39. pmc Identifying candidate genes affecting developmental time in Drosophila melanogaster: pervasive pleiotropy and gene-by-environment interaction
    Julián Mensch
    Departamento de Ecologia, Genetica y Evolucion, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Argentina
    BMC Dev Biol 8:78. 2008
    ..The present work is one of the first systematic studies of the genetic basis of developmental time, in which we also evaluate the impact of environmental variation on the expression of the trait...
  40. ncbi There is no regulatory role for induced nitric oxide in the regulation of the in vitro proliferative response of bovine mononuclear cells to mitogens, alloantigens or superantigens
    H J Schuberth
    Immunology Unit, School of Veterinary Medicine, Hannover, Germany
    Immunobiology 198:439-50. 1998
    ..to investigate whether it modulates their proliferative response following allogeneic (mixed leukocyte cultures, aMLC), mitogenic (PWM, Con A) or superantigenic (SEA, SEB) stimulation...
  41. ncbi Inhibitors of the mitogen-activated protein kinase kinase 1/2 signaling pathway clear prion-infected cells from PrPSc
    Elin K Nordström
    Department of Neuroscience, Karolinska Institutet, SE 171 77 Stockholm, Sweden
    J Neurosci 25:8451-6. 2005
    ..factor (BDNF), stimulates the formation of PrPSc in a gonadotropin-releasing hormone-secreting neuronal cell line (GT1-1 cells) infected with the Rocky Mountain Laboratory (RML) strain of scrapie as determined by Western blot analysis...
  42. ncbi Effect of Bcl-2 overexpression on mitochondrial structure and function
    Alicia J Kowaltowski
    Departamento de Bioquimica, Instituto de Quimica, Universidade de Sao Paulo, Sao Paulo, SP, 05508 900, Brazil
    J Biol Chem 277:42802-7. 2002
    ..respiration, membrane potential, deltapH, and intramitochondrial [K+] in digitonin-permeabilized PC12 and GT1-7 neural cells that either do not express human Bcl-2 (control transfectants) or that were transfected with and ..
  43. ncbi Genomic cloning of ribonucleases in Nicotiana glutinosa leaves, as induced in response to wounding or to TMV-infection, and characterization of their promoters
    Takeshi Hayashi
    Laboratory of Biochemistry, Department of Bioscience and Biotechnology, Faculty of Agriculture, Graduate School, Kyushu University, Fukuoka, Japan
    Biosci Biotechnol Biochem 67:2574-83. 2003
    ..cis-regulating elements, such as the elicitor responsiveness element: the W-box, a TMV responsive element: GT1, and the WUN-motif at positions between -401 and -174...
  44. ncbi [Effects of nitriles and amides on the growth and the nitrile hydratase activity of the Rhodococcus sp. strain gt1]
    A Iu Maksimov
    Institute of Ecology and Genetics of Microorganisms, Ural Division, Russian Academy of Sciences, ul Pushkina 1 57, Perm, 614081 Russia
    Prikl Biokhim Mikrobiol 39:63-8. 2003
    ..2.1.84) activity of the Rhodococcus sp. strain gt1 isolated from soil were studied...
  45. ncbi Gastro-oesophageal and bile reflux--simultaneous quantitative assessment with gastric and gallbladder emptying evaluation: clinical applicability of a new computerized gammagraphic method
    P M da Costa
    Cirurgia I Propedêutica Cirúrgica, Faculdade de Medicina de Lisboa, Portugal
    Nucl Med Commun 13:817-23. 1992
    ..001). Patients with oesophagitis have a tendency for slower gastric emptying patterns (gastric emptying T1/2, GT1/2 P < 0.05). The calculated specificity for gallbladder emptying parameters was 77...
  46. ncbi Astrocyte protection of neurons: role of transforming growth factor-beta signaling via a c-Jun-AP-1 protective pathway
    Krishnan M Dhandapani
    Institute of Molecular Medicine and Genetics, Program in Neurobiology, and Department of Neurology, School of Medicine, Medical College of Georgia, Augusta, Georgia 30912, USA
    J Biol Chem 278:43329-39. 2003
    ..The results of the study show that soluble factors in astrocyte-conditioned media (ACM) protect murine GT1-7 neurons from serum deprivation-induced cell death and that this neuroprotection is correlated with enhanced ..
  47. doi Determination of ganglioside composition and structure in human brain hemangioma by chip-based nanoelectrospray ionization tandem mass spectrometry
    Catalin Schiopu
    Mass Spectrometry Laboratory, National Institute for Research and Development in Electrochemistry and Condensed Matter, Str Plautius Andronescu Nr 1, 300224, Timisoara, Romania
    Anal Bioanal Chem 395:2465-77. 2009
    ..In HFC, only GT1 (d18:1/20:0), and GT3 (d18:1/25:1) polysialylated species were detected...
  48. ncbi Expression of three gene families encoding cell-cell communication molecules in the prepubertal nonhuman primate hypothalamus
    A E Mungenast
    Division of Neuroscience, Oregon National Primate Research Center, Oregon Health and Sciences University, Beaverton, OR, USA
    J Neuroendocrinol 17:208-19. 2005
    ..Examination of LHRH neurones, using the LHRH-secreting cell line GT1-7, showed that these cells contain transcripts encoding NRXs and one of their ligands (NRL1), at least one ..
  49. ncbi Expression and signal transduction pathways of endothelin receptors in neuroendocrine cells
    S S Stojilkovic
    Endocrinology and Reproduction Research Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892, USA
    Front Neuroendocrinol 17:327-69. 1996
    ..parathyroid glands, pancreas, ovary, uterus, placenta, and prostate, while only ETA receptors are expressed in GT1 neurons, anterior pituitary cells, alpha T3-1 immortalized gonadotropes, parathyroid-derived cells, thyrocytes, ..
  50. ncbi Expression of the fusion glycoprotein of Newcastle disease virus in transgenic rice and its immunogenicity in mice
    Zhen Quan Yang
    College of Bioscience and Biotechnology and Jiangsu Key Laboratory of Crop Genetics and Physiology, Yangzhou University, 12 East Wenhui Road, Yangzhou, Jiangsu 225009, PR China
    Vaccine 25:591-8. 2007
    ..gene of Newcastle disease virus (NDV F) under the control of maize ubiquitin (Ubi) promoter or rice glutelin (Gt1) promoter, respectively, were constructed, and introduced into rice (Oryzy sativa L...
  51. ncbi Genomic structure and transcriptional regulation of the human growth hormone secretagogue receptor
    S Petersenn
    IHF Institute for Hormone and Fertility Research, University of Hamburg, Germany
    Endocrinology 142:2649-59. 2001
    ..was detected in monkey kidney COS-7 cells, human endometrium Skut-1B cells, mouse hypothalamic LHRH neuronal GT1-7 cells, or mouse corticotroph pituitary AtT20 cells...
  52. ncbi Stimulation of luteinizing hormone-releasing hormone (LHRH) gene expression in GT1-7 cells by its metabolite, LHRH-(1-5)
    T J Wu
    Department of Obstetrics and Gynecology, Uniformed Services University of the Health Sciences, Bethesda, Maryland 20814, USA
    Endocrinology 146:280-6. 2005
    ..The present study supports the hypothesis that the physiological metabolite of LHRH, LHRH-(1-5), is functionally capable of regulating the reproductive neuroendocrine system...
  53. ncbi Exonic splicing enhancer-dependent splicing of the gonadotropin-releasing hormone premessenger ribonucleic acid is mediated by tra2alpha, a 40-kilodalton serine/arginine-rich protein
    Jae Young Seong
    Hormone Research Center, Chonnam National University, Kwangju 500 757, Korea
    Mol Endocrinol 16:2426-38. 2002
    ..In the presence of an ESE in exon 4 (ESE4), an addition of GT1 NE further increased the excision rate of intron A, whereas the addition of KK1 (a non-GnRH-producing cell) NE ..
  54. ncbi The promoter of the TLC1.1 retrotransposon from Solanum chilense is activated by multiple stress-related signaling molecules
    Marcela Salazar
    Instituto de Biologia Vegetal y Biotecnologia, Universidad de Talca, Casilla 747, Talca, Chile
    Plant Cell Rep 26:1861-8. 2007
    ..Three putative cis-acting elements MRE, GCN4 and GT1/TCA identified in the P270 promoter may be involved in the PERE box-independent activation pathway...
  55. ncbi Activation of the melanocortin-4 receptor mobilizes intracellular free calcium in immortalized hypothalamic neurons
    Erika A Newman
    Section of Gastrointestinal Surgery, Department of Surgery, University of Michigan Medical Center, Ann Arbor, MI 48109 0346, USA
    J Surg Res 132:201-7. 2006
    ..To further understand its function, we hypothesized that the melanocortin-4 receptor activates the Galphaq/phospholipase C signaling pathway, resulting in alterations of cytoplasmic calcium in immortalized hypothalamic (GT1-1) neurons.
  56. ncbi Epidemiology and pathogenicity of African bat lyssaviruses
    W Markotter
    Department of Microbiology and Plant Pathology, Faculty of Natural and Agricultural Sciences, University of Pretoria, South Africa
    Dev Biol (Basel) 131:317-25. 2008
    ..over the past four years from Epomophorus fruit bats and from incidental terrestrial animals and (4) Rabies virus (gt1) - with two virus biotypes endemic in mongoose and in canid species (mostly domestic dogs, jackals and bat-eared ..
  57. doi Leptin infusion and obesity in mouse cause alterations in the hypothalamic melanocortin system
    Johann Gout
    INSERM U855, Lyon, France
    Obesity (Silver Spring) 16:1763-9. 2008
    ..This hypothesis was reinforced after in vitro studies, using the mouse hypothalamic GT1-7 cell line, since both leptin and Norleucine(4), D-Phenylalanine(7)-alpha-MSH (NDP-alpha-MSH) treatments increased ..
  58. doi Globular adiponectin regulates energy homeostasis through AMP-activated protein kinase-acetyl-CoA carboxylase (AMPK/ACC) pathway in the hypothalamus
    Jun Ping Wen
    Department of Endocrinology, Fujian Provincial Hospital, Fujian Medical University, Fuzhou, China
    Mol Cell Biochem 344:109-15. 2010
    ..We investigated the possible mechanisms involved in appetite regulation by adiponectin in vitro with GT1-7 cells, a mouse immortalized hypothalamic neuron...
  59. pmc Dynamic genetic interactions determine odor-guided behavior in Drosophila melanogaster
    Deepa Sambandan
    Department of Genetics, The W M Keck Center for Behavioral Biology, North Carolina State University, Raleigh 27695 7617, USA
    Genetics 174:1349-63. 2006
    ..We performed a screen of 1339 co-isogenic p[GT1]-element insertion lines to identify novel genes that contribute to odor-guided behavior and identified 55 ..
  60. pmc Silencing of RASSF3 by DNA hypermethylation is associated with tumorigenesis in somatotroph adenomas
    Hu Peng
    Department of Otolaryngology Head and Neck Surgery, Changzheng Hospital, Second Military Medical University, Shanghai, China
    PLoS ONE 8:e59024. 2013
    ..RASSF3 hypermethylation and downregulation was also observed in rat GH3 and mouse GT1.1 somatotroph adenoma cell lines...
  61. pmc Poplar carbohydrate-active enzymes. Gene identification and expression analyses
    Jane Geisler-Lee
    Department of Forest Genetics and Plant Physiology, Umea Plant Science Center, Sweden
    Plant Physiol 140:946-62. 2006
    ..most families were proportionally increased, xylan and pectin-related families were underrepresented and the GT1 family of secondary metabolite-glycosylating enzymes was overrepresented in poplar...
  62. pmc Verification of an assay for quantification of hepatitis C virus RNA by use of an analyte-specific reagent and two different extraction methods
    Michael S Forman
    Division of Microbiology, The Johns Hopkins Medical Institutions, Meyer B1 193, 600 North Wolfe St, Baltimore, MD 21287 7093, USA
    J Clin Microbiol 42:3581-8. 2004
    ..2.0. For HCV genotype 1 (Gt1), the lower limits of quantification after manual extraction were slightly lower than those for automated ..
  63. ncbi A role for hypothalamic malonyl-CoA in the control of food intake
    Zhiyuan Hu
    Department of Biological Chemistry, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    J Biol Chem 280:39681-3. 2005
    ..ribonucleoside (AICAR), a 5'-AMP kinase activator, rapidly lowers malonyl-CoA both in GT1-7 hypothalamic neurons and in the hypothalami of mice...
  64. ncbi Melanocortin-4 receptor-mediated inhibition of apoptosis in immortalized hypothalamic neurons via mitogen-activated protein kinase
    Biaoxin Chai
    Department of Surgery, University of Michigan, Ann Arbor, MI, USA
    Peptides 27:2846-57. 2006
    The melanocortin-4 receptor (MC4R) is a seven transmembrane member of the melanocortin receptor family. The GT1-1 cell line exhibits endogenous expression of MC4R...
  65. pmc Riluzole inhibits spontaneous Ca2+ signaling in neuroendocrine cells by activation of K+ channels and inhibition of Na+ channels
    Luis Beltran-Parrazal
    Department of Neurology, UCLA School of Medicine, 710 Westwood Plaza, Los Angeles, CA 90095 1769, USA
    Br J Pharmacol 140:881-8. 2003
    ..and reversibly inhibited spontaneous Ca2+ oscillations in both immortalized GnRH-secreting hypothalamic neurons (GT1 cells) and in the prolactin and growth-hormone-secreting GH3 cell line...
  66. ncbi DHEA and DHEA sulfate differentially regulate neural androgen receptor and its transcriptional activity
    Qianxing Mo
    Department of Biological Sciences, Lehigh University, 111 Research Drive, Bethlehem, PA 18015, USA
    Brain Res Mol Brain Res 126:165-72. 2004
    ..included the ability to (1) up-regulate neural androgen receptor (AR) protein level in mouse brain and immortalized GT1-7 hypothalamic cells and (2) assess their effect on reporter gene expression through AR in CV-1 cells cotransfected ..
  67. ncbi Cell differentiation of gonadotropin-releasing hormone neurons and alternative RNA splicing of the gonadotropin-releasing hormone transcript
    Youngshik Choe
    NRL Development and Neuroendocrine Research Group, School of Biological Sciences, Seoul National University, Seoul, Korea
    Neuroendocrinology 77:282-90. 2003
    ..Using the immortalized GnRH-producing GT1 cell line, we found that activation of protein kinase C (PKC) with 12-O-tetradecanoylphorbol-13-acetate induces ..
  68. ncbi Estradiol enhances the neurotoxicity of glutamate in GT1-7 cells through an estrogen receptor-dependent mechanism
    Rei Cheng Yang
    Department of Physiology, Kaohsiung Medical University, No 100, Shih Chuan 1st Road, Kaohsiung 807, Taiwan, ROC
    Neurotoxicology 24:65-73. 2003
    ..estradiol modulates the neurotoxic effect of glutamate on the neurons through estrogen receptor (ER), immortalized GT1-7 cells, which simultaneously express ER and NMDAR were used...
  69. ncbi Selective roles of protein kinase C isoforms on cell motility of GT1 immortalized hypothalamic neurones
    Y Choe
    School of Biological Sciences, Seoul National University, Seoul, Korea
    J Neuroendocrinol 15:508-15. 2003
    ..that activation of the protein kinase C (PKC) signalling pathway promoted morphological differentiation of GT1 hypothalamic neurones via an increase in beta-catenin, a cell-cell adhesion molecule, indicating a possible ..
  70. pmc Daily changes in GT1-7 cell sensitivity to GnRH secretagogues that trigger ovulation
    Sheng Zhao
    Department of Psychology, and Helen Wills Neuroscience Institute, University of California, Berkeley, CA 94720 1650, USA
    Neuroendocrinology 89:448-57. 2009
    ..To test this possibility, we examined changes in the sensitivity of immortalized GnRH neurons, GT1-7 cells, to timed stimulation by two key neuropeptides thought to trigger ovulation on the afternoon of proestrus, ..
  71. ncbi Expression of circadian rhythm genes in gonadotropin-releasing hormone-secreting GT1-7 neurons
    Julia M A Gillespie
    Department of Physiology, University of Toronto, Toronto, Ontario, Canada M5S 1A8
    Endocrinology 144:5285-92. 2003
    ..Melatonin also plays a role in reproduction, including direct effects on GnRH-secreting GT1-7 neurons...
  72. ncbi Regulation of endogenous melanocortin-4 receptor expression and signaling by glucocorticoids
    Julien A Sebag
    Department of Pharmacology and Physiology, University of Rochester Medical Center, Rochester, New York 14642, USA
    Endocrinology 147:5948-55. 2006
    ..We investigated the effect of glucocorticoids on endogenous MC4 receptors expressed in GT1-1 cells, an immortalized hypothalamic neuronal cell line...
  73. pmc Metabolic capacity and myosin expression in single muscle fibres of the garter snake
    R S Wilkinson
    Department of Cell Biology and Physiology, Washington University School of Medicine, St Louis, MO 63110
    J Physiol 440:113-29. 1991
    ..5. Based on whole-muscle analysis, two myosin alkali light chains, denoted ALC1 and ALC2, and one myosin regulatory light chain were present...
  74. pmc Intact lipid rafts regulate HIV-1 Tat protein-induced activation of the Rho signaling and upregulation of P-glycoprotein in brain endothelial cells
    Yu Zhong
    Department of Neurosurgery, Molecular Neuroscience and Vascular Biology Laboratory, University of Kentucky Medical Center, Lexington, Kentucky 40536, USA
    J Cereb Blood Flow Metab 30:522-33. 2010
    ..The present data indicate the critical function of intact lipid rafts and the Rho signaling in HIV-1-mediated upregulation of P-gp and potential development of drug resistance in brain endothelial cells...
  75. ncbi Estrogen-astrocyte-luteinizing hormone-releasing hormone signaling: a role for transforming growth factor-beta(1)
    C D Buchanan
    Department of Physiology and Endocrinology, School of Medicine, Medical College of Georgia, Augusta, Georgia 30912 3000, USA
    Biol Reprod 62:1710-21. 2000
    ..Exposure of LHRH-secreting (GT1-7) cells to conditioned media (CM) from C6 glial cells and hypothalamic astrocytes (HA) stimulated LHRH release...
  76. ncbi Spectral characterization of the voltage-sensitive dye di-4-ANEPPDHQ applied to probing live primary and immortalized neurons
    Yu Wang
    Center for Optical Technologies, Department of Electrical and Computer Engineering, Lehigh University, Bethlehem, PA 18015, USA
    Opt Express 17:984-90. 2009
    ..and bound to the plasma membrane of primary rat hippocampal neurons and immortalized mouse hypothalamic neurons (GT1-7) in vitro. Their dependence on the local chemical and electrical environment of dye molecules was determined...
  77. pmc Mouse neuronal cells expressing exogenous bovine PRNP and simultaneous downregulation of endogenous mouse PRNP using siRNAs
    Sang Gyun Kang
    Department of Infectious Diseases, College of Veterinary Medicine, KRF Zoonotic Disease Priority Research Institute and BK21 Program for Veterinary Science, Seoul National University, Seoul, Republic of Korea
    Prion 4:32-7. 2010
    ..To establish a BSE-specific in vitro cell culture model, N2a and GT1 mouse neuronal cell lines were generated to express the bovine prion protein by transfection of the bovine prion ..
  78. pmc A role for chloroplast-localized Thylakoid Formation 1 (THF1) in bacterial speck disease development
    Tamding Wangdi
    Plant Biology Division, The Samuel Roberts Noble Foundation, Ardmore, OK, USA
    Plant Signal Behav 5:425-7. 2010
    ..We designated these genes as altered COR response (ALC). When silenced, one gene designated ALC1 produced a hypersensitive/necrosis-like phenotype after COR application in a coronatine insensitive 1 (COI1)-..
  79. doi Suprachiasmatic nucleus neurons display endogenous resistance to excitotoxicity
    Kathleen Bottum
    Department of Medicine, Division of Internal Medicine and Psychiatry, Southern Illinois School of Medicine, Springfield, IL 62794 9636, USA
    Exp Biol Med (Maywood) 235:237-46. 2010
    ..2 than in the control hypothalamic neurons (GT1-7)...
  80. pmc Differential fibroblast growth factor 8 (FGF8)-mediated autoregulation of its cognate receptors, Fgfr1 and Fgfr3, in neuronal cell lines
    Natasha N Mott
    Department of Cell and Molecular Physiology, Loyola University Chicago Stritch School of Medicine, Maywood, Illinois, United States of America
    PLoS ONE 5:e10143. 2010
    ..HT-22 cells), and neuroendocrine cells derived from hypothalamic gonadotropin-releasing hormone (GnRH) neurons (GT1-7 cells)...
  81. pmc Neurogenetic networks for startle-induced locomotion in Drosophila melanogaster
    Akihiko Yamamoto
    Department of Zoology, North Carolina State University, Raleigh, NC 27695, USA
    Proc Natl Acad Sci U S A 105:12393-8. 2008
    ..In a screen of 720 lines of D. melanogaster, carrying single P[GT1] transposon insertions, we found 267 lines that showed significant changes in startle-induced locomotor behavior...
  82. ncbi Estrogen regulates KiSS1 gene expression through estrogen receptor alpha and SP protein complexes
    Dali Li
    Institute of Biosciences and Technology, Texas A and M Health Science Center, 2121 West Holcombe Boulevard, Houston, TX 77030, USA
    Endocrinology 148:4821-8. 2007
    ..gene was up-regulated with the administration of E(2) in estrogen receptor alpha (ERalpha)-positive hypothalamic GT1-7 cells...
  83. pmc Regulation of ghrelin signaling by a leptin-induced gene, negative regulatory element-binding protein, in the hypothalamic neurons
    Tadasuke Komori
    Department of Anatomy and Neurobiology, Wakayama Medical University, 811 1 Kimiidera, Wakayama 641 8509, Japan
    J Biol Chem 285:37884-94. 2010
    ..reporter assays showed that NREBP repressed GHS-R promoter activity in a hypothalamic neuronal cell line, GT1-7, and its repressive activity was abolished by the replacement of negative regulatory element in GHS-R promoter...
  84. pmc Dynamic chromatin modifications control GnRH gene expression during neuronal differentiation and protein kinase C signal transduction
    Anita K Iyer
    Department of Reproductive Medicine, University of California, San Diego, 9500 Gilman Drive, La Jolla, California 92093 0674, USA
    Mol Endocrinol 25:460-73. 2011
    ..Only in GT1-7 mature GnRH neuronal cells did GnRH regulatory elements display high sensitivity to DNase and enrichment of ..
  85. pmc Mutational analysis of the necdin gene in patients with congenital isolated hypogonadotropic hypogonadism
    Daiane Beneduzzi
    Unidade de Endocrinologia do Desenvolvimento, Laboratório de Hormônios e Genética Molecular LIM42, Hospital das Clinicas da Faculdade de Medicina da Universidade de Sao Paulo, Avenida Doutor Eneas de Carvalho Aguiar 155, 2 andar, Bloco 6, CEP 05403 900 São Paulo, Sao Paulo, Brazil
    Eur J Endocrinol 165:145-50. 2011
    ..In humans, necdin plays a potential role in the hypogonadotropic hypogonadism phenotype in patients with Prader-Willi syndrome...
  86. ncbi Oral immunization with transgenic rice seeds expressing VP2 protein of infectious bursal disease virus induces protective immune responses in chickens
    Jianxiang Wu
    State Key Laboratory of Rice Biology, Institute of Biotechnology, Zhejiang University, Hangzhou 31009, China
    Plant Biotechnol J 5:570-8. 2007
    ..The VP2 cDNA of IBDV strain ZJ2000 was cloned downstream of the Gt1 promoter of the rice glutelin GluA-2 gene in the binary expression vector, pCambia1301-Gt1...
  87. pmc Immunologic evidence for lack of heterologous protection following resolution of HCV in patients with non-genotype 1 infection
    Julian Schulze zur Wiesch
    Partners AIDS Research Center, Infectious Disease Division, Massachusetts General Hospital and Harvard Medical School, Boston, MA, USA
    Blood 110:1559-69. 2007
    ..Surprisingly, more HCV-specific CD4(+) T-cell responses were detected in patients with chronic non-GT1 infection compared with patients with chronic GT1 infection (P = .017)...
  88. doi The glycosylphosphatidylinositol anchor is a major determinant of prion binding and replication
    Clive Bate
    Department of Pathology and Infectious Diseases, Royal Veterinary College, Hawkshead Lane, North Mymms, Herts AL9 7TA, UK
    Biochem J 428:95-101. 2010
    ..In the present manuscript we report that soluble PrPSc bound to and replicated within both GT1 neuronal cells and primary cortical neurons...
  89. ncbi Retinoic acid- and phorbol ester-induced neuronal differentiation down-regulates caveolin expression in GnRH neurons
    Cristina D'Orlando
    Department of Experimental Medicine, University of Milano Bicocca, Monza, Italy
    J Neurochem 104:1577-87. 2008
    GN11 and GT1-7 are immortalized gonadotropin-releasing hormone-positive murine cell lines exhibiting the features of immature olfactory neurons and differentiated hypothalamic neurons, respectively...
  90. doi Lagos bat virus virulence in mice inoculated by the peripheral route
    W Markotter
    Department of Microbiology and Plant Pathology, University of Pretoria, South Africa
    Epidemiol Infect 137:1155-62. 2009
    Lagos bat virus (LBV) constitutes genotype (gt) 2 in the Lyssavirus genus. In contrast to the gt1 lyssavirus, rabies virus (RABV), LBV was reported to have markedly reduced levels of peripheral pathogenicity...
  91. pmc Cell death mechanisms in GT1-7 GnRH cells exposed to polychlorinated biphenyls PCB74, PCB118, and PCB153
    Sarah M Dickerson
    Division of Pharmacology and Toxicology, University of Texas at Austin, Austin, TX 78712, USA
    Toxicol Appl Pharmacol 237:237-45. 2009
    ..We used an immortalized hypothalamic GT1-7 cell line, which synthesizes the neuroendocrine peptide gonadotropin-releasing hormone (GnRH), to examine the ..
  92. doi Antiprion properties of prion protein-derived cell-penetrating peptides
    Kajsa Löfgren
    Department of Biochemistry and Biophysics, The Arrhenius Laboratories, Stockholm University, SE 106 91 Stockholm, Sweden
    FASEB J 22:2177-84. 2008
    ..Healthy (GT1-1) and scrapie-infected (ScGT1-1) mouse neuronal hypothalamic cells were treated with various CPPs, including the ..
  93. ncbi Underlying mechanism of actions of tefluthrin, a pyrethroid insecticide, on voltage-gated ion currents and on action currents in pituitary tumor (GH3) cells and GnRH-secreting (GT1-7) neurons
    Sheng Nan Wu
    Department of Physiology, National Cheng Kung University Medical College, Tainan, Taiwan
    Toxicology 258:70-7. 2009
    ..Its effects on membrane ion currents in pituitary tumor (GH3) cells and in hypothalamic (GT1-7) neurons were investigated...
  94. doi Intracellular oxidative stress and cytotoxicity in rat primary cortical neurons exposed to cholesterol secoaldehyde
    Achuthan C Raghavamenon
    Department of Environmental Toxicology, Southern University and A and M College, Baton Rouge, LA 70813, USA
    Biochem Biophys Res Commun 386:170-4. 2009
    ..ChSeco or 3beta-hydroxy-5-oxo-5,6-secocholestan-6-al) has been shown to induce Abeta aggregation and apoptosis in GT1-7 hypothalamic neurons...
  95. pmc Pertussis toxin-sensitive signaling of melanocortin-4 receptors in hypothalamic GT1-7 cells defines agouti-related protein as a biased agonist
    Thomas R H Büch
    Walther Straub Institut für Pharmakologie und Toxikologie, Ludwig Maximilians Universitat Munchen, 80336 Munchen
    J Biol Chem 284:26411-20. 2009
    ..Analyzing a hypothalamic cell line (GT1-7 cells), we observed activation of pertussis toxin-sensitive G proteins by the wild-type MC4R as well, reflecting ..
  96. pmc A rapid accurate culture assay for infectivity in Transmissible Encephalopathies
    Ying Liu
    Section of Neuropathology, Yale Medical School, New Haven, CT 06510, USA
    J Neurovirol 14:352-61. 2008
    ..Because we found GT1 hypothalamic cells, unlike neuroblastoma N2a clones, were highly susceptible to a variety of TSE agents, and could ..
  97. pmc Enterostatin alters protein trafficking to inhibit insulin secretion in Beta-TC6 cells
    Miejung Park
    Center for Advanced Nutrition, Utah State University, 4715 Old Main Hill, Logan, UT 84322 4715, USA
    Peptides 30:1866-73. 2009
    ..of the genomic response of both a human hepatoma cell line (HepG2 cells) and a mouse hypothalamic cell line (GT1-7 cells) to enterostatin suggested that it might regulate protein trafficking...
  98. ncbi Gene expression profile of quinacrine-cured prion-infected mouse neuronal cells
    Carlo Fasano
    Unité de Trafic Membranaire et Pathogénèse, Institut Pasteur, Paris, France
    J Neurochem 105:239-50. 2008
    ..to avoid the complexities of shifting gene expression in a heterogeneous cell population, we used a single clone of GT1 cells that was de novo infected with mouse prion-infected brain homogenate and then treated with quinacrine to ..
  99. doi Pharmacological analysis of human D1 AND D2 dopamine receptor missense variants
    Munya A Al-Fulaij
    Sackler School of Graduate Biomedical Sciences, Tufts University School of Medicine, Boston, MA 02111, USA
    J Mol Neurosci 34:211-23. 2008
    ..After expression in either HEK293 or neuronal GT1 cells, basal and ligand-induced signaling of each of these receptors was determined and compared to wild type...
  100. ncbi Hyperglycaemic hyperosmolar nonketotic state as a cause of low gonadotrophin levels in postmenopausal diabetic women: a role for severe hypernatraemia
    J Lado-Abeal
    Unidade de Enfermedades Tiroideas e Metabolicas, Departamento de Medicina, Complejo Hospitalario Universitario de Santiago de Compostela, Universidad de Santiago de Compostela, Spain
    J Neuroendocrinol 19:983-7. 2007
    ..hyperglycaemia, hypernatraemia or both inhibit in vitro gonadotrophin-releasing hormone (GnRH) expression in GT1-7 neurones...
  101. doi On diamond surface properties and interactions with neurons
    P Ariano
    Nanostructured Surface and Interface Excellence Centre NIS, University of Torino, Torino, Italy
    Eur Phys J E Soft Matter 30:149-56. 2009
    ..Different surface terminations were induced through exposure to atomic hydrogen and to intense UV irradiation. GT1-7 cells, a neuronal line of hypothalamic origin, were plated directly onto the diamond surfaces without exogenous ..

Research Grants83

  1. NEUROSECRETORY GENE EXPRESSION IN THE HYPOTHALAMUS
    PAMELA MELLON; Fiscal Year: 1993
    ..We plan a systematic analysis of the responses to known modulatory agents in the GnRH neuronal cen line, GT1. Our second goal is to unravel the genetic control mechanisms which specify GnRH expression to an exclusive ..
  2. NEUROSECRETORY GENE EXPRESSION IN THE HYPOTHALAMUS
    Pamela L Mellon; Fiscal Year: 2011
    ..We will utilize two major model systems: our immortalized GnRH-secreting hypothalamic cells (GT1) and genetically manipu- lated mice...
  3. NEUROSECRETORY GENE EXPRESSION IN THE HYPOTHALAMUS
    PAMELA MELLON; Fiscal Year: 2007
    ..We will utilize two major model systems: our immortalized GnRH-secreting hypothalamic cells (GT1) and genetically manipulated mice...
  4. Circadian Regulation of Gonadotropin-Releasing Hormone
    Patrick E Chappell; Fiscal Year: 2010
    ..Studies in the previous funding period, using GnRH-secreting GT1-7 cell lines and transgenic mouse models, demonstrated that perturbations in GnRH-specific clocks can modulate ..
  5. B-Synuclein as a Treatment for Lewy Body Disease
    Eliezer Masliah; Fiscal Year: 2005
    ..To test this hypothesis, alpha-synuclein- overexpressing GT1-7 and B103 neuronal cells will be co- transfected with beta-synuclein GT1-7 cells will be evaluated for cell ..
  6. MOLECULAR MECHANISM OF PHOTORECEPTOR G PROTEIN SIGNALING
    Nikolai O Artemyev; Fiscal Year: 2010
    ..The properties and regulation of rod transducin Gt1 during phototransduction are understood to a far greater extent than those of cone transducin Gt2...
  7. Functional Characterization of Leishmania Transporters
    Scott Landfear; Fiscal Year: 2007
    ..The second aim will investigate the specific functions of the 3 glucose transporter isoforms GT1, GT2, and GT3...
  8. CELLULAR PROTEIN MATURATION AND DEGRADATION
    Daniel Hebert; Fiscal Year: 2009
    ..The UDP-glucose: glycoprotein glucosyltransferase (GT1) is currently the only known protein that can modify the glycan structure dependent upon the properties of the ..
  9. Functional Characterization of Leishmania Transporters
    Scott M Landfear; Fiscal Year: 2010
    ..The second aim will investigate the specific functions of the 3 glucose transporter isoforms GT1, GT2, and GT3...
  10. CELLULAR PROTEIN MATURATION AND DEGRADATION
    Daniel N Hebert; Fiscal Year: 2010
    ..The UDP-glucose: glycoprotein glucosyltransferase (GT1) is currently the only known protein that can modify the glycan structure dependent upon the properties of the ..
  11. BIOCHEMISTRY OF EUKARYOTIC MESSENGER RNA SYNTHESIS
    RONALD C contact CONAWAY; Fiscal Year: 2010
    ..ATP-dependent chromatin remodelers referred to as the human INO80 complex and the SNF2 family member and oncogene ALC1 (Activated in Liver Cancer 1)...
  12. NEUROSECRETORY GENE EXPRESSION IN THE HYPOTHALAMUS
    PAMELA MELLON; Fiscal Year: 2005
    ..We will utilize two model systems: our immortalized GnRH-secreting hypothalamic cell line (GT1) and genetically manipulated mice...
  13. ONTOGENY AND EXPRESSION OF THE CFTR IN BRAIN
    ANDREW MULBERG; Fiscal Year: 2003
    ..Control mechanisms of neurosecretion will be examined in the fetal brain, and GT1-7, a GnRH-expressing hypothalamic neuronal cell line...
  14. Multiple circadian oscillators: Regulation of a neuroendocrine rhythm
    Sandra J Legan; Fiscal Year: 2010
    ..Furthermore, immortalized GnRH neurons, GT1-7 cells, exhibit circadian rhythms of clock gene expression in vitro...
  15. Multiple circadian oscillators: Regulation of a neuroendocrine rhythm
    MARILYN J contact DUNCAN; Fiscal Year: 2011
    ..Furthermore, immortalized GnRH neurons, GT1-7 cells, exhibit circadian rhythms of clock gene expression in vitro...
  16. Multiple circadian oscillators: Regulation of a neuroendocrine rhythm
    MARILYN DUNCAN; Fiscal Year: 2009
    ..Furthermore, immortalized GnRH neurons, GT1-7 cells, exhibit circadian rhythms of clock gene expression in vitro...
  17. DEVELOPMENTAL CONTROL OF GNRH EXPRESSION BY POU PROTEINS
    Margaret Wierman; Fiscal Year: 2002
    ..The POU-domain family members, SCIP and Brn-4 were cloned from GT1-7 neuronal cells...
  18. A Molecular Dynamics Approach to GnRH Pulsatility
    FREDRIC BOOCKFOR; Fiscal Year: 2006
    ..Recent development of GT1 clonal neuronal cultures that release GnRH in a pulsatile manner provided an excellent model system to study this ..
  19. NOVEL FACTORS THAT REGULATE GNRH NEURONAL DEVELOPMENT
    Margaret Wierman; Fiscal Year: 2002
    ..ability to study the regulation of GnRH synthesis was advanced with the development of GnRH producing cell lines: GT1-7 cells derived from postmigratory GnRH neurons in the forebrain that make abundant GnRH, and Gn10 cells derived ..
  20. Circadian Regulation of Gonadotropin-Releasing Hormone
    PATRICK CHAPPELL; Fiscal Year: 2007
    ..in this laboratory demonstrates that not only are all molecular clock components expressed in the GnRH-secreting GT1-7 cell line, but that transcripts oscillate in these cultured cells with a circadian period...
  21. Circadian Regulation of Gonadotropin-Releasing Hormone
    PATRICK CHAPPELL; Fiscal Year: 2005
    ..in this laboratory demonstrates that not only are all molecular clock components expressed in the GnRH-secreting GT1-7 cell line, but that transcripts oscillate in these cultured cells with a circadian period...
  22. Circadian Clock Regulation of Pulsatile GnRH Secretion
    PATRICK CHAPPELL; Fiscal Year: 2005
    ..that not only are all molecular clock components, such as mPer1, mPer2, and clock present in the GnRH-secreting GT1-7 cell line, but that transcripts of mPer1 and mPer2 oscillate in these cultured cells with a circadian period...
  23. APOPROTEIN E AND NEURITE OUTGROWTH
    GODFREY GETZ; Fiscal Year: 2001
    ..The goal of this proposal is to examine the influence of apoE4 on neurite outgrowth using GT1-1 trk-9 cells exposed to exogenous apoE or stably expressing apoE in the cells...