Genomes and Genes
Gene Symbol: MXI1
Description: MAX interactor 1, dimerization protein
Alias: MAD2, MXD2, MXI, bHLHc11, max-interacting protein 1, MAX dimerization protein 2, Max-related transcription factor, class C basic helix-loop-helix protein 11
Publications240 found, 100 shown here
- Mutation of the MXI1 gene in prostate cancerL R Eagle
Department of Pediatrics, Children s Hospital of Pittsburgh, Pennsylvania 15213, USA
Nat Genet 9:249-55. 1995The Mxi1 protein negatively regulates Myc oncoprotein activity and thus potentially serves a tumour suppressor function. MXI1 maps to chromosome 10q24-q25, a region that is deleted in some cases of prostate cancer...
- An amino-terminal domain of Mxi1 mediates anti-Myc oncogenic activity and interacts with a homolog of the yeast transcriptional repressor SIN3N Schreiber-Agus
Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, New York 10461
Cell 80:777-86. 1995..of Myc-responsive genes in which transactivation-competent Myc-Max heterodimers are opposed by repressive Mxi1-Max or Mad-Max complexes...
- Localization of the human Mxi1 transcription factor gene (MXI1) to chromosome 10q24-q25D S Wechsler
Department of Pediatrics, Johns Hopkins University School of Medicine, Baltimore, Maryland 21287
Genomics 21:669-72. 1994
- Mapping of two genes encoding members of a distinct subfamily of MAX interacting proteins: MAD to human chromosome 2 and mouse chromosome 6, and MXI1 to human chromosome 10 and mouse chromosome 19S Edelhoff
Department of Pathology, University of Washington, Seattle 98195
Oncogene 9:665-8. 1994Both the MAD and the MXI1 genes encode basic-helix-loop-helix-leucine zipper (bHLH-Zip) transcription factors which bind Max in vitro, forming a sequence-specific DNA-binding complex similar to the Myc-Max heterodimer...
- Mxi1, a protein that specifically interacts with Max to bind Myc-Max recognition sitesA S Zervos
Department of Genetics, Harvard Medical School, Boston, Massachusetts 02114
Cell 72:223-32. 1993We used the interaction trap to isolate a novel human protein that specifically interacts with Max. This protein, Mxi1 (for Max interactor 1), contains a bHLH-Zip motif that is similar to that found in Myc family proteins...
- Role of Mxi1 in ageing organ systems and the regulation of normal and neoplastic growthN Schreiber-Agus
Department of Molecular Genetics, Albert Einstein College of Medicine, Bronx, New York 10461, USA
Nature 393:483-7. 1998b>Mxi1 belongs to the Mad (Mxi1) family of proteins, which function as potent antagonists of Myc oncoproteins...
- Commonly occurring loss and mutation of the MXI1 gene in prostate cancerE V Prochownik
Section of Hematology Oncology, Children s Hospital of Pittsburgh, Pennsylvania 15213, USA
Genes Chromosomes Cancer 22:295-304. 1998..We previously demonstrated that the MXI1 gene maps to 10q24-q25 and is mutated in some tumors with cytogenetically detectable deletions of this locus...
- Mxi1 inhibits the proliferation of U87 glioma cells through down-regulation of cyclin B1 gene expressionI Manni
Istituto Regina Elena, Centro Ricerca Sperimentale, Laboratorio di Oncogenesi Molecolare, Via delle Messi d Oro 156, 00158 Rome, Italy
Br J Cancer 86:477-84. 2002b>Mxi1 is a Mad family member that plays a role in cell proliferation and differentiation. To test the role of Mxi1 on tumorigenesis of glioma cells we transfected a CMV-driven MXI1 cDNA in U87 human glioblastoma cells...
- miR-24-3p and miR-27a-3p promote cell proliferation in glioma cells via cooperative regulation of MXI1Weiyi Xu
Key Laboratory of Gene Engineering of the Ministry of Education, State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat Sen University, Guangzhou, P R China
Int J Oncol 42:757-66. 2013..By integrated bioinformatic analysis and experimental confirmation, we identified MXI1, which has been found to act as a tumor suppressor gene by affecting câ€‘Myc, as a direct target of miRâ..
- Merotelic kinetochore orientation is a major mechanism of aneuploidy in mitotic mammalian tissue cellsD Cimini
Department of Biology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA
J Cell Biol 153:517-27. 2001..They also had inactivated mitotic spindle checkpoint activities since they did not label with either Mad2 or 3F3/2 antibodies...
- Influence of follicular fluid meiosis-activating sterol on aneuploidy rate and precocious chromatid segregation in aged mouse oocytesS Cukurcam
Research Laboratories of Schering AG, Berlin, Germany
Hum Reprod 22:815-28. 2007..Reduced expression of cohesion and checkpoint proteins and predivision has been hypothesized to occur in age-related aneuploidy in oocytes...
- The first mitosis of the mouse embryo is prolonged by transitional metaphase arrestMarta Sikora-Polaczek
Department of Embryology, Institute of Zoology, Warsaw University, 02 096 Warsaw, Poland
Biol Reprod 74:734-43. 2006..The end of prometaphase is correlated with checkpoint inactivation and disappearance of MAD2L1 (MAD2) and RSN (CLIP-170) proteins from kinetochores...
- The role of pre- and post-anaphase microtubules in the cytokinesis phase of the cell cycleJ C Canman
Department of Biology, University of North Carolina Chapel Hill, North Carolina 27599, USA
Curr Biol 10:611-4. 2000..Here, we have taken advantage of the discovery that microinjection of antibodies to the spindle checkpoint protein Mad2 (mitotic arrest deficient) in prometaphase abrogates the spindle checkpoint, producing premature chromosome ..
- Control of chromosome stability by the beta-TrCP-REST-Mad2 axisDaniele Guardavaccaro
Department of Pathology, NYU Cancer Institute, New York University School of Medicine, 550 First Avenue, MSB 599, New York, New York 10016, USA
Nature 452:365-9. 2008..the ubiquitin ligase SCF(beta-TrCP) during the G2 phase of the cell cycle to allow transcriptional derepression of Mad2, an essential component of the spindle assembly checkpoint...
- Mlp1 acts as a mitotic scaffold to spatially regulate spindle assembly checkpoint proteins in Aspergillus nidulansColin P De Souza
Department of Molecular Genetics, Ohio State University, Columbus, 43210, USA
Mol Biol Cell 20:2146-59. 2009..In interphase, An-Mlp1 tethers the An-Mad1 and An-Mad2 SAC proteins to NPCs...
- Ribonuclease activity of Dis3 is required for mitotic progression and provides a possible link between heterochromatin and kinetochore functionHiroaki Murakami
CREST Research Program, Japan Science and Technology Corporation, Department of Gene Mechanisms, Graduate School of Biostudies, Kyoto University, Yoshida Honmachi, Sakyo ku, Kyoto, Japan
PLoS ONE 2:e317. 2007..Previous isolation of the dis3-54 cold-sensitive mutant of fission yeast Schizosaccharomyces pombe suggested that Dis3 is also required for correct chromosome segregation...
- Molecular and cellular mechanisms of invasion of the intestinal barrier by enteric pathogens. The paradigm of ShigellaP J Sansonetti
Unite de Pathogenie Microbienne Moleculaire, INSERM U 389, Institut Pasteur, Paris, France
Folia Microbiol (Praha) 43:239-46. 1998..e. invasins) through a specialized, activable, type-III secretory apparatus (i.e. Mxi/Spa)...
- Visualization of Mad2 dynamics at kinetochores, along spindle fibers, and at spindle poles in living cellsB J Howell
Department of Biology, CB 3280, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA
J Cell Biol 150:1233-50. 2000..A key checkpoint component is the mitotic arrest-deficient protein 2 (Mad2), which localizes to unattached kinetochores and inhibits activation of the anaphase-promoting complex (APC) ..
- Mad2 is required for inhibiting securin and cyclin B degradation following spindle depolymerisation in meiosis I mouse oocytesHayden A Homer
Newcastle Fertility Centre at Life, International Centre for Life, Times Square, Newcastle upon Tyne, NE1 4EP, UK
Reproduction 130:829-43. 2005b>Mad2 is a pivotal component of the spindle assembly checkpoint (SAC) which inhibits anaphase promoting complex/cyclo-some (APC/C) activity by sequestering Cdc20 thereby regulating the destruction of securin and cyclin B...
- Cell cycle-dependent interaction of Mad2 with conserved Box1/2 region of human granulocyte-macrophage colony-stimulating factor receptor common betacM Takeda
Department of Molecular and Developmental Biology, Institute of Medical Science, Core Research for Evolutional Science and Technology, Japan
J Biol Chem 276:41803-9. 2001..The mouse homologue of Mad2 protein, which plays an important role in the M phase of the cell cycle, was revealed to associate with the box1/2 ..
- Anaphase onset does not require the microtubule-dependent depletion of kinetochore and centromere-binding proteinsJulie C Canman
University of North Carolina, Department of Biology, 607 Fordham Hall, CB3280, Chapel Hill, NC 27599, USA
J Cell Sci 115:3787-95. 2002Spindle checkpoint proteins, such as Mad2 and BubR1, and the motors dynein/dynactin and CENP-E usually leave kinetochores prior to anaphase onset by microtubule-dependent mechanisms...
- Smurf2 as a novel mitotic regulator: From the spindle assembly checkpoint to tumorigenesisEvan C Osmundson
Department of Molecular Pharmacology and Biological Chemistry, Northwestern University Feinberg School of Medicine, 303 E, Chicago Avenue, Chicago, IL 60611, USA
Cell Div 4:14. 2009..The critical effector of the spindle checkpoint, Mitotic arrest deficient 2 (Mad2), is recruited to unattached kinetochores forming a complex with other regulatory proteins to efficiently and ..
- The corepressor N-CoR and its variants RIP13a and RIP13Delta1 directly interact with the basal transcription factors TFIIB, TAFII32 and TAFII70G E Muscat
University of Queensland, Centre for Molecular and Cellular Biology, Ritchie Research Laboratories, B402A, St Lucia 4072, Queensland, Australia
Nucleic Acids Res 26:2899-907. 1998..g. TR, RAR), the orphan nuclear receptors (e.g. Rev-erbAalpha/beta), Mxi-1 and Mad bHLH-zip proteins and the oncoproteins PLZF and LAZ3/BCL6 is mediated by the corepressors N-CoR and SMRT...
- Mammalian p55CDC mediates association of the spindle checkpoint protein Mad2 with the cyclosome/anaphase-promoting complex, and is involved in regulating anaphase onset and late mitotic eventsM Kallio
Department of Cell Biology, Health Sciences Center, University of Virginia, Charlottesville, Virginia 22908, USA
J Cell Biol 141:1393-406. 1998..p55CDC coimmunoprecipitates with three important elements of the M phase checkpoint machinery: Cdc27, Cdc16, and Mad2. p55CDC is required for binding Mad2 with the Cdc27 and Cdc16...
- Determinants of conformational dimerization of Mad2 and its inhibition by p31cometMarina Mapelli
Department of Experimental Oncology, European Institute of Oncology, Milan, Italy
EMBO J 25:1273-84. 2006..The ubiquitous SAC constituents Mad1 and Mad2 are recruited to kinetochores in prometaphase...
- Changing Mad2 levels affects chromosome segregation and spindle assembly checkpoint control in female mouse meiosis IThĂ©odora Niault
CNRS UMR7622 Biologie du DĂ©veloppement, Paris, France
PLoS ONE 2:e1165. 2007..Prolonged metaphase arrest due to overexpression of Mad2 has been shown to be deleterious to the ensuing anaphase, leading to the generation of aneuploidies and ..
- FAT10 plays a role in the regulation of chromosomal stabilityJianwei Ren
Division of Medical Sciences, National Cancer Center, Singapore 169610, Singapore
J Biol Chem 281:11413-21. 2006..Here we show that FAT10 interacts with MAD2, a spindle checkpoint protein, during mitosis...
- Cytoplasmic dynein/dynactin drives kinetochore protein transport to the spindle poles and has a role in mitotic spindle checkpoint inactivationB J Howell
Department of Biology, University of North Carolina, Chapel Hill, NC 27599, USA
J Cell Biol 155:1159-72. 2001..the microtubule motors CENP-E and cytoplasmic dynein, and proteins involved with the mitotic spindle checkpoint, Mad2, Bub1R, and the 3F3/2 phosphoantigen...
- Differences in spindle association of the mitotic checkpoint protein Mad2 in mammalian spermatogenesis and oogenesisM Kallio
Turku Center for Biotechnology, BioCity, 5th Floor, TykistĂ¶katu 6B, Turku, FIN 20520, Finland
Dev Biol 225:112-23. 2000We have investigated expression and subcellular localization of the spindle checkpoint protein Mad2 during rat and mouse spermatogenesis and in superovulated mouse oocytes...
- Novel mad2 alleles isolated in a Schizosaccharomyces pombe gamma-tubulin mutant are defective in metaphase arrest activity, but remain functional for chromosome stability in unperturbed mitosisYoshie Tange
Kazusa DNA Research Institute, Kisarazu, Chiba 292 0818, Japan
Genetics 175:1571-84. 2007..In the absence of the spindle assembly checkpoint gene, mad2+, the spindle dynamics in the gamma-tubulin mutant were greatly compromised, leading to a high incidence of ..
- Mad2 and BubR1 function in a single checkpoint pathway that responds to a loss of tensionKatie B Shannon
SPIRE fellow, Department of Biology, University of North Carolina, Chapel Hill, North Carolina 27599 3280, USA
Mol Biol Cell 13:3706-19. 2002..The metaphase delay at 23 degrees C was abrogated by injection of Mad2 inhibitors, showing that Mad2 and the spindle checkpoint were responsible for the prolonged metaphase...
- Mad2-independent spindle assembly checkpoint activation and controlled metaphase-anaphase transition in Drosophila S2 cellsBernardo Orr
Instituto de Biologia Molecular e Celular, 4150 180 Porto, Portugal
Mol Biol Cell 18:850-63. 2007..We analyzed the role of the putative Drosophila Mad2 homologue in the spindle assembly checkpoint and mitotic progression...
- Microinjection of antibody to Mad2 protein into mammalian cells in mitosis induces premature anaphaseG J Gorbsky
Department of Cell Biology, University of Virginia, Charlottesville, Virginia 22908, USA
J Cell Biol 141:1193-205. 1998In yeast, the Mad2 protein is required for the M phase arrest induced by microtubule inhibitors, but the protein is not essential under normal culture conditions...
- Checkpoint protein BubR1 acts synergistically with Mad2 to inhibit anaphase-promoting complexGuowei Fang
Department of Biological Sciences, Stanford University, Stanford, California 94305 5020, USA
Mol Biol Cell 13:755-66. 2002..In response to activation of the checkpoint, Mad2 binds to and inhibits Cdc20-APC...
- MxiE regulates intracellular expression of factors secreted by the Shigella flexneri 2a type III secretion systemColleen D Kane
Department of Microbiology and Immunology, F Edward Hebert School of Medicine, Uniformed Services University of the Health Sciences, Bethesda, Maryland 20814 4799, USA
J Bacteriol 184:4409-19. 2002The mxi-spa locus on the virulence plasmid of Shigella flexneri encodes components of the type III secretion system...
- An Inv/Mxi-Spa-like type III protein secretion system in Burkholderia pseudomallei modulates intracellular behaviour of the pathogenMark P Stevens
Division of Environmental Microbiology, Institute for Animal Health, Compton Laboratory, Berkshire RG20 7NN, UK
Mol Microbiol 46:649-59. 2002..pseudomallei and host cells. The Bsa system is very similar to the Inv/Mxi-Spa type III secretion systems of Salmonella and Shigella. Moreover, B...
- Nuf2 and Hec1 are required for retention of the checkpoint proteins Mad1 and Mad2 to kinetochoresJennifer G DeLuca
Department of Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
Curr Biol 13:2103-9. 2003..results in a strong prometaphase block with an active spindle checkpoint, which correlates with low but detectable Mad2 at kinetochores that have no or few stable kinetochore microtubules...
- Attenuated virulence and protective efficacy of a Burkholderia pseudomallei bsa type III secretion mutant in murine models of melioidosisMark P Stevens
Division of Microbiology, Institute for Animal Health, Compton Laboratory, Berkshire RG20 7NN, UK
Microbiology 150:2669-76. 2004..An Inv/Mxi-Spa-like type III protein secretion apparatus, encoded by the B...
- A Tel1/MRX-dependent checkpoint inhibits the metaphase-to-anaphase transition after UV irradiation in the absence of Mec1Michela Clerici
Dipartimento di Biotecnologie e Bioscienze, Universita degli Studi di Milano Bicocca, P zza della Scienza 2, 20126 Milan, Italy
Mol Cell Biol 24:10126-44. 2004..The spindle assembly checkpoint protein Mad2 also partially contributes to the G(2)/M arrest of UV-irradiated mec1Delta cells independently of Rad53 ..
- Cytoplasmic dynein participates in meiotic checkpoint inactivation in mouse oocytes by transporting cytoplasmic mitotic arrest-deficient (Mad) proteins from kinetochores to spindle polesDong Zhang
State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100080, China
Reproduction 133:685-95. 2007..meiosis and its relationship with two major spindle checkpoint proteins, mitotic arrest-deficient (Mad) 1 and Mad2. Oocytes at various stages during the first meiosis were fixed and immunostained for dynein, Mad1, Mad2, ..
- The spindle checkpoint functions of Mad3 and Mad2 depend on a Mad3 KEN box-mediated interaction with Cdc20-anaphase-promoting complex (APC/C)Matylda Sczaniecka
Wellcome Trust Centre for Cell Biology, Institute of Cell Biology, University of Edinburgh, EH9 3JR, United Kingdom
J Biol Chem 283:23039-47. 2008..In the fission yeast, Schizosaccharomyces pombe, we find that the Mad3 and Mad2 spindle checkpoint proteins interact stably with the APC/C in mitosis...
- Epstein-Barr virus nuclear antigen 2 disrupts mitotic checkpoint and causes chromosomal instabilityShih Hsuan Pan
Graduate Institute of Microbiology, College of Medicine, National Taiwan University, Taipei 100, Taiwan
Carcinogenesis 30:366-75. 2009..EBNA2 was shown to be able to downregulate mitotic arrest deficient 2 (MAD2) approximately 2- to 3-fold and upregulate polo-like kinase 1 (PLK1) approximately 2-fold...
- Unattached kinetochores catalyze production of an anaphase inhibitor that requires a Mad2 template to prime Cdc20 for BubR1 bindingAnita Kulukian
Ludwig Institute for Cancer Research, University of California at San Diego, La Jolla, CA 92093, USA
Dev Cell 16:105-17. 2009..Using physiologically relevant levels of Mad2, Bub3, BubR1, and Cdc20, we demonstrate that unattached kinetochores on purified chromosomes catalytically generate ..
- Checkpoint signals in grasshopper meiosis are sensitive to microtubule attachment, but tension is still essentialR B Nicklas
Department of Biology, Duke University, Durham, NC 27708 1000, USA
J Cell Sci 114:4173-83. 2001..The checkpoint is activated by attachment-sensitive components including Mad2 and certain phosphorylated proteins detected by the 3F3/2 antibody...
- Requirement of chromatid cohesion proteins rad21/scc1 and mis4/scc2 for normal spindle-kinetochore interaction in fission yeastYusuke Toyoda
Department of Gene Mechanisms, Graduate School of Biostudies, 606 8502, Kyoto, Japan
Curr Biol 12:347-58. 2002..The failure to form cohesion in the S phase results in premature separation of chromatids in G2/M. Mitotic kinetochores free from microtubules or the lack of tension are known to activate spindle checkpoint...
- Loss of heterozygosity and microsatellite instability on chromosome arm 10q in neuroblastomaPaula LĂˇzcoz
Department of Health Sciences, Public University of Navarra, 31006 Pamplona, Spain
Cancer Genet Cytogenet 174:1-8. 2007..We screened the 10q locus for loss of heterozygosity and the promoter methylation status of PTEN, MGMT, MXI1, and FGFR2 in neuroblastic tumors and neuroblastoma cell lines...
- The HECT E3 ligase Smurf2 is required for Mad2-dependent spindle assembly checkpointEvan C Osmundson
Department of Molecular Pharmacology and Biological Chemistry, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USA
J Cell Biol 183:267-77. 2008..Smurf2 depletion results in enhanced polyubiquitination and degradation of Mad2, a critical checkpoint effector...
- Induction of type III secretion in Shigella flexneri is associated with differential control of transcription of genes encoding secreted proteinsB Demers
UnitĂ© de PathogĂ©nie Microbienne and UnitĂ© INSERM, Institut Pasteur, Paris, France
EMBO J 17:2894-903. 1998..Entry of Shigella into epithelial cells involves a type III secretion machinery, encoded by the mxi and spa operons, and the IpaA-D secreted proteins...
- Induction of max by adrenomedullin and calcitonin gene-related peptide antagonizes endothelial apoptosisM Shichiri
Second Department of Internal Medicine, Tokyo Medical and Dental University, Japan
Mol Endocrinol 13:1353-63. 1999..is a basic-helix-loop-helix-leucine zipper protein that forms heterodimers with its alternative partners, Mad and Mxi-1, to behave as an antagonist for Myc-Max heterodimer through competition for common DNA targets...
- Control of localization of a spindle checkpoint protein, Mad2, in fission yeastAmy E Ikui
Department of Radiation Oncology, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461, USA
J Cell Sci 115:1603-10. 2002..b>Mad2, a component of the checkpoint, targets fission yeast Slp1/budding yeast Cdc20/human p55CDC and prevents it from ..
- Mouse Sin3A interacts with and can functionally substitute for the amino-terminal repression of the Myc antagonist Mxi1G Rao
Department of Cell Biology, Albert Einstein College of Medicine, Bronx, New York 10461, USA
Oncogene 12:1165-72. 1996b>Mxi1 is a basic region helix-loop-helix leucine zipper (bHLH/LZ) protein that, in association with Max, antagonizes Myc oncogenic activities...
- Insights into mad2 regulation in the spindle checkpoint revealed by the crystal structure of the symmetric mad2 dimerMaojun Yang
Department of Pharmacology, The University of Texas Southwestern Medical Center, Dallas, Texas, United States of America
PLoS Biol 6:e50. 2008In response to misaligned sister chromatids during mitosis, the spindle checkpoint protein Mad2 inhibits the anaphase-promoting complex or cyclosome (APC/C) through binding to its mitotic activator Cdc20, thus delaying anaphase onset...
- Kinetic analysis of Mad2-Cdc20 formation: conformational changes in Mad2 are catalyzed by a C-Mad2-ligand complexLatesh Lad
Cytokinetics Inc, South San Francisco, California 94080, USA
Biochemistry 48:9503-15. 2009Structural changes in the mitotic arrest deficient protein 2 (Mad2) have been proposed to be essential for spindle checkpoint function...
- MAD2 downregulation in hypoxia is independent of promoter hypermethylationMaria Prencipe
UCD School of Medicine and Medical Science SMMS, University College Dublin, Belfield, Dublin, Ireland
Cell Cycle 9:2856-65. 2010Aberrant expression of the MAD2 protein has been linked to chromosomal instability, malignant transformation and chemoresistance...
- Assignment of the human MAD and MXI1 genes to chromosomes 2p12-p13 and 10q24-q25D N Shapiro
Department of Experimental Oncology, St Jude Children s Research Hospital, Memphis, Tennessee 38101
Genomics 23:282-5. 1994MAD and MXI1, two recently described members of the basic helix-loop-helix (bHLH) gene family, encode proteins that dimerize with and modulate the DNA binding of max...
- The LAZ3(BCL-6) oncoprotein recruits a SMRT/mSIN3A/histone deacetylase containing complex to mediate transcriptional repressionP Dhordain
U124 INSERM IRCL, Place de Verdun, F 59045 Lille Cedex, France and Howard Hughes Medical Institute and The Salk Institute for Biological Studies, La Jolla, CA 92037, USA
Nucleic Acids Res 26:4645-51. 1998..For instance, the bHLH-Zip transcriptional repressors MAD/MXI recruit HDACs together with the mSIN3 corepressors, whereas unliganded nuclear receptors contact another ..
- IpgD, a protein secreted by the type III secretion machinery of Shigella flexneri, is chaperoned by IpgE and implicated in entry focus formationK Niebuhr
Unite de Pathogenie Microbienne Moleculaire, INSERM U389, Institut Pasteur, 28 rue du Dr Roux, 75724 Paris Cedex 15, France
Mol Microbiol 38:8-19. 2000..Entry of bacteria into non-phagocytic cells requires the IpaA-D proteins that are secreted by the Mxi-Spa type III secretion machinery...
- Partially functional Cenpa-GFP fusion protein causes increased chromosome missegregation and apoptosis during mouse embryogenesisPaul Kalitsis
Murdoch Childrens Research Institute, Royal Children s Hospital, Flemington Road, Parkville 3052, Melbourne, Victoria, Australia
Chromosome Res 11:345-57. 2003..that bind to a host of centromere-specific structural and mitotic spindle checkpoint proteins (Cenpc, BubR1, Mad2 and Zw10)...
- Mad2 prevents aneuploidy and premature proteolysis of cyclin B and securin during meiosis I in mouse oocytesHayden A Homer
Newcastle Fertility Centre at Life, Centre for Life, Newcastle upon Tyne NE1 4EP, United Kingdom
Genes Dev 19:202-7. 2005In mitosis, the spindle checkpoint protein Mad2 averts aneuploidy by delaying anaphase onset until chromosomes align. Here we show that depletion of Mad2 in meiosis I mouse oocytes induced an increased incidence of aneuploidy...
- Genomic models of metastatic cancer: functional analysis of death-from-cancer signature genes reveals aneuploid, anoikis-resistant, metastasis-enabling phenotype with altered cell cycle control and activated Polycomb Group (PcG) protein chromatin silencinGennadi V Glinsky
Functional Genomics Laboratory, Ordway Cancer Center, Ordway Research Institute, Inc, Center for Medical Sciences, Division of Urology, Albany Medical College, New York 12208 USA
Cell Cycle 5:1208-16. 2006..protein (IAP) family (Survivin and XIAP); activation of mitotic spindle check point proteins (BUB1, BUB3, KNTC2, Mad2, PLK1, PLK4, STK6/Aurora A); and elevated levels of certain cell cycle control/marker proteins (CCNB1, CCNB2, CCND1,..
- In vivo analysis of chromosome condensation in Saccharomyces cerevisiaeAmit C J Vas
Department of Genetics, Cell Biology, and Development, University of Minnesota Medical School, Minneapolis, MN 55455, USA
Mol Biol Cell 18:557-68. 2007..progression by activation of the spindle assembly checkpoint caused a loss of condensation that was dependent on Mad2, followed by a delayed loss of cohesion between sister chromatids...
- Mechanisms of mitotic cell death induced by chemotherapy-mediated G2 checkpoint abrogationCelia Vogel
Institute for Molecular Biology and Tumor Research, Philipps University of Marburg, Emil Mannkopff Strasse 2, D 35037 Marburg, Germany
Cancer Res 67:339-45. 2007..treatment results in a mitotic arrest that requires an active mitotic spindle checkpoint, involving the function of Mad2, Bub1, BubR1, Mps1, Aurora B, and survivin...
- Two different mitotic checkpoint inhibitors of the anaphase-promoting complex/cyclosome antagonize the action of the activator Cdc20Esther Eytan
Unit of Biochemistry, Rappaport Faculty of Medicine, Technion Israel Institute of Technology, Haifa 31096, Israel
Proc Natl Acad Sci U S A 105:9181-5. 2008..complex (MCC) inhibitor of APC/C composed of the APC/C activator Cdc20 associated with the checkpoint proteins Mad2, BubR1, and Bub3...
- Effects of heat stress during in vitro maturation on cytoplasmic versus nuclear components of mouse oocytesJun Zuo Wang
Laboratory for Animal Reproduction and Embryology, College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Shandong Province, People s Republic of China
Reproduction 137:181-9. 2009..While spindle assembling was normal and no MAD2 was activated in oocytes matured at 37 or 40 degrees C, spindle assembling was affected and MAD2 was activated in ..
- Methylation of p16INK4A and mitotic arrest defective protein 2 (MAD2) genes in gastric marginal-zone B-cell lymphomasSanghui Park
Department of Pathology, Gachon University of Medicine and Science, Gil Medical Center, Incheon, Republic of Korea
Acta Haematol 120:217-24. 2008Products of cyclin-dependent kinase inhibitor p16(INK4A)and mitotic arrest defective protein 2 (MAD2) genesare key regulator proteins at the G1 restriction point and mitotic checkpoint of the cell cycle...
- Dynein light intermediate chain 1 is required for progress through the spindle assembly checkpointMylavarapu V S Sivaram
Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, MA 01605, USA
EMBO J 28:902-14. 2009..Metaphase delay and increased interkinetochore distances are suppressed by depletion of Mad1, Mad2 or BubR1 or by re-expression of wtLIC1 or a Cdk1 site phosphomimetic LIC1 mutant, but not Cdk1-phosphorylation-..
- Heat shock factor 1-mediated aneuploidy requires a defective function of p53Eun Ho Kim
Division of Radiation Effects, Korea Institute of Radiological and Medical Sciences, Seoul, Korea
Cancer Res 69:9404-12. 2009..Moreover, the interaction affinity of Cdc20 with Mad2 was inhibited in p53Mut cells, whereas the interaction between Cdc20 and HSF1 was increased...
- ATP is required for the release of the anaphase-promoting complex/cyclosome from inhibition by the mitotic checkpointShirly Miniowitz-Shemtov
Unit of Biochemistry, Rappaport Faculty of Medicine, Technion Israel Institute of Technology, Haifa 31096, Israel
Proc Natl Acad Sci U S A 107:5351-6. 2010..APC/C is inhibited by a mitotic checkpoint complex (MCC) composed of BubR1, Bub3, Mad2, and Cdc20...
- Small-molecule kinase inhibitors provide insight into Mps1 cell cycle functionNicholas Kwiatkowski
Department of Cancer Biology, Dana Farber Cancer Institute, Boston, Massachusetts, USA
Nat Chem Biol 6:359-68. 2010..Consistent with RNAi studies, chemical inhibition of Mps1 leads to defects in Mad1 and Mad2 establishment at unattached kinetochores, decreased Aurora B kinase activity, premature mitotic exit and gross ..
- Replication-compromised cells require the mitotic checkpoint to prevent tetraploidizationZilai Zhang
Department of Pathology, Stanford University Medical Center, Edwards Building, Room R270, 300 Pasteur Drive, Stanford, CA 94305 5324, USA
Chromosoma 120:73-82. 2011..the mutant PolÎ±-protein failed to sustain mitotic arrest and, when propagated progressively, down-regulated Mad2 and BubR1 and accumulated 4N-DNA from the 2N-DNA cells...
- DNA methylation profiling of medulloblastoma allows robust subclassification and improved outcome prediction using formalin-fixed biopsiesEdward C Schwalbe
Northern Institute for Cancer Research, Newcastle University, Sir James Spence Institute Level 5, Royal Victoria Infirmary, Newcastle upon Tyne, NE1 4LP, UK
Acta Neuropathol 125:359-71. 2013..b>MXI1 and IL8 methylation were identified as novel independent high-risk biomarkers in cross-validated survival models of ..
- A synthetic dolastatin 10 analogue suppresses microtubule dynamics, inhibits cell proliferation, and induces apoptotic cell deathPraveen Kumar Gajula
CSIR Central Drug Research Institute, Lucknow 226 001 India
J Med Chem 56:2235-45. 2013..D5 increased the accumulation of checkpoint proteins BubR1 and Mad2 at the kinetochoric region and caused G2/M block in these cells...
- MYC and MXI1 protein expression: potential prognostic significance in women with breast cancer in ChinaLin Ping Xu
The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China
Oncol Res Treat 37:118-23. 2014To investigate the expression levels and the clinical significance of MYC and MXI1 proteins in breast cancer.
- Mitotic checkpoint proteins Mad1 and Mad2 - structural and functional relationship with implication in genetic diseasesSperanta Avram
Anatomy, Animal Physiology and Biophysics Department, Faculty of Biology, University of Bucharest, 91 95th Independentei Str, Sector 5, Bucharest 050095, Romania
Curr Comput Aided Drug Des 10:168-81. 2014..deeply involved in correct cell divisions, but some of these, such as mitotic arrest-deficient proteins (Mad1 and Mad2), are critical...
- Spindle assembly checkpoint protein expression correlates with cellular proliferation and shorter time to recurrence in ovarian cancerBarbara McGrogan
UCD School of Medicine and Medical Science, University College Dublin, UCD, Belfield, Dublin 4, Ireland UCD Conway Institute of Biomolecular and Biomedical Research, University College Dublin, Belfield, Dublin 4, Ireland National Cancer Control Programme, King s Inns House, Dublin 1, Ireland Electronic address
Hum Pathol 45:1509-19. 2014..BUB1-related protein kinase (BUBR1) and mitotic arrest deficient 2 (MAD2), essential spindle assembly checkpoint components, play a key role in response to taxanes...
- MicroRNA 28 controls cell proliferation and is down-regulated in B-cell lymphomasChristof Schneider
Institute for Cancer Genetics
Proc Natl Acad Sci U S A 111:8185-90. 2014..of miR-28 impairs cell proliferation and clonogenic properties of BL cells by modulating several targets including MAD2 mitotic arrest deficient-like 1, MAD2L1, a component of the spindle checkpoint whose down-regulation is essential ..
- Stranglehold on the spindle assembly checkpoint: the human papillomavirus E2 protein provokes BUBR1-dependent aneuploidyChye Ling Tan
a Cell Cycle Control in Skin Epidermis Institute of Medical Biology A Star, Biopolis Immunos, Singapore
Cell Cycle 14:1459-70. 2015..This phenotype is associated with interaction of E2 with the Mitotic Checkpoint Complex (MCC) proteins Cdc20, MAD2 and BUBR1...
- The Spindle Assembly Checkpoint Is Not Essential for Viability of Human Cells with Genetically Lowered APC/C ActivityThomas Wild
Proteomics Program, The Novo Nordisk Foundation Center for Protein Research, Faculty of Health and Medical Sciences, University of Copenhagen, Blegdamsvej 3b, 2200 Copenhagen, Denmark
Cell Rep 14:1829-40. 2016..transition and, strikingly, renders cells insensitive to chemical inhibition of MPS1 and genetic ablation of MAD2, both of which are essential for the SAC...
- RING domain is essential for the antiviral activity of TRIM25 from orange spotted grouperYing Yang
Key Laboratory of Tropical Marine Bio resources and Ecology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, 164 West Xingang Road, Guangzhou 510301, China University of Chinese Academy of Sciences, Beijing, China
Fish Shellfish Immunol 55:304-14. 2016..signaling molecules, including interferon regulatory factor (IRF) 3, interferon-induced 35-kDa protein (IFP35), MXI, IRF7 and myeloid differentiation factor 88 (MyD88), suggesting that the positive regulation of interferon immune ..
- The Bfa1/Bub2 GAP complex comprises a universal checkpoint required to prevent mitotic exitY Wang
Verna and Mars McLean Department of Biochemistry and Molecular Biology, Howard Hughes Medical Institute, Baylor College of Medicine, Houston, Texas 77030, USA
Curr Biol 10:1379-82. 2000..cdc13-1 bub2 and cdc13-1 bfa1 but not cdc13-1 mad2 double mutants rebud and reduplicate their DNA at the restrictive temperature...
- Significance of MAD2 expression to mitotic checkpoint control in ovarian cancer cellsXianghong Wang
Department of Anatomy, Faculty of Medicine, University of Hong Kong, Hong Kong
Cancer Res 62:1662-8. 2002..Mitotic checkpoint controls are thought to be essential for accurate chromosomal segregation, and MAD2 is a key component of this checkpoint...
- Identification of a MAD2-binding protein, CMT2, and its role in mitosisToshiyuki Habu
Department of Radiation Oncology, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461, USA
EMBO J 21:6419-28. 2002b>MAD2 is a key component of the spindle checkpoint that delays the onset of anaphase until all the kinetochores are attached to the spindle...
- The role of Cdc55 in the spindle checkpoint is through regulation of mitotic exit in Saccharomyces cerevisiaeChristopher M Yellman
Department of Biochemistry and Molecular Genetics, University of Virginia Medical Center, University of Virginia, Charlottesville, VA 22908, USA
Mol Biol Cell 17:658-66. 2006..pathways, one leading to inhibition of the metaphase-to-anaphase transition by checkpoint proteins, including Mad2, and the other to inhibition of mitotic exit by Bub2. We show that Cdc55 is a negative regulator of mitotic exit...
- RNA Interference as a tool to study the function of MAD2 in mouse oocyte meiotic maturationJian Ying Wang
Key Laboratory of Animal Resistance Research, College of Life Science, Shandong Normal University, Jinan, PR China
Mol Reprod Dev 74:116-24. 2007..As a member of spindle checkpoint proteins, MAD2 takes a central role in the regulation of anaphase onset and genome integrity...
- A functional genomic screen identifies a role for TAO1 kinase in spindle-checkpoint signallingViji M Draviam
Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
Nat Cell Biol 9:556-64. 2007..TAO1 interacts with the checkpoint kinase BubR1 and promotes enrichment of the checkpoint protein Mad2 at sites of defective attachment, providing evidence for a regulatory step that precedes the proposed Mad2-Mad1 ..
- Spindle formation, chromosome segregation and the spindle checkpoint in mammalian oocytes and susceptibility to meiotic errorE Vogt
University of Bielefeld, Faculty of Biology, Gene Technology Microbiology, Bielefeld, Germany
Mutat Res 651:14-29. 2008..in the presence of unattached chromosomes, associated with accumulation of checkpoint components, in particular, Mad2, at the kinetochores...
- Prognostic significance of O6-methylguanine DNA methyltransferase and p57 methylation in patients with diffuse large B-cell lymphomasSun Mi Lee
Department of Pathology, The Catholic University of Korea, Seoul, Korea
APMIS 117:87-94. 2009..institute (n=44) were studied for methylation of tumor-related genes, MGMT, p15(INK4B), p16(INK4A), p16(INK4A), Mad2, TMS1/ASC, CASP8, and GSTP1. The clinical behavior of DLBCL after chemotherapy was followed up and analyzed...
- Hydrophobic bile acid-induced micronuclei formation, mitotic perturbations, and decreases in spindle checkpoint proteins: relevance to genomic instability in colon carcinogenesisClaire M Payne
Department of Cell Biology and Anatomy, College of Medicine, University of Arizona, Tucson, AZ 85724 5044, USA
Nutr Cancer 62:825-40. 2010..basis for these mitosis-related perturbations included decreased expression of the spindle checkpoint proteins, Mad2, BubR1, and securin...
- Knockdown of Mad2 induces osteosarcoma cell apoptosis-involved Rad21 cleavageLing Yu
Department of Orthopedics, Renmin Hospital, Wuhan University, Wuhan, Hubei, China
J Orthop Sci 16:814-20. 2011Besides Mad2's role in carcinogenesis, recent study has shown that it is essential in cell survival...
- Closed MAD2 (C-MAD2) is selectively incorporated into the mitotic checkpoint complex (MCC)Aaron R Tipton
Department of Biological Sciences, University of Toledo, Toledo, OH, USA
Cell Cycle 10:3740-50. 2011..b>MAD2 is an evolutionarily conserved mitotic checkpoint protein that exists in open (O) and closed (C) conformations...
- Aurora kinase-A inactivates DNA damage-induced apoptosis and spindle assembly checkpoint response functions of p73Hiroshi Katayama
Department of Molecular Pathology, The University of Texas M D Anderson Cancer Center, Houston, TX 77054, USA
Cancer Cell 21:196-211. 2012..Aurora-A phosphorylated p73 also facilitates inactivation of SAC through dissociation of the MAD2-CDC20 complex in cells undergoing mitosis...
- Involvement of multiple cell cycle aberrations in early preneoplastic liver cell lesions by tumor promotion with thioacetamide in a two-stage rat hepatocarcinogenesis modelMasayuki Kimura
Laboratory of Veterinary Pathology, Tokyo University of Agriculture and Technology, 3 5 8 Saiwai cho, Fuchu shi, Tokyo 183 8509, Japan
Exp Toxicol Pathol 65:979-88. 2013..of cell proliferation marker Ki-67, G2/M and spindle checkpoint proteins (phosphorylated checkpoint kinase 1 and Mad2), the DNA damage-related protein phosphorylated histone H2AX, and G2-M phase-related proteins (topoisomerase IIÎ±, ..
- A direct role of Mad1 in the spindle assembly checkpoint beyond Mad2 kinetochore recruitmentThomas Kruse
The Novo Nordisk Foundation Center for Protein Research, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark
EMBO Rep 15:282-90. 2014..A key component of the SAC is the Mad2 protein, which can adopt either an inactive open (O-Mad2) or active closed (C-Mad2) conformation...
- Molecular dynamics simulation on the conformational transition of the mad2 protein from the open to the closed stateChaoqun Li
College of Chemistry, Beijing Normal University, 19 Xinjiekouwai Street, Beijing 100875, China
Int J Mol Sci 15:5553-69. 2014The Mad2 protein, with two distinct conformations of open- and closed-states, is a key player in the spindle checkpoint. The closed Mad2 state is more active than the open one...
- Mps1Mph1 Kinase Phosphorylates Mad3 to Inhibit Cdc20Slp1-APC/C and Maintain Spindle Checkpoint ArrestsJudith Zich
Wellcome Trust Centre for Cell Biology, Institute of Cell Biology, University of Edinburgh, Edinburgh, United Kingdom
PLoS Genet 12:e1005834. 2016..This genetic dissection of APC/C inhibition demonstrates that Mps1(Mph1) kinase-dependent modifications of Mad3 and Mad2 act in a concerted manner to maintain spindle checkpoint arrests.
- Dimethyl Sulfoxide Perturbs Cell Cycle Progression and Spindle Organization in Porcine Meiotic OocytesXuan Li
College of Animal Science and Technology, Northeast Agricultural University, Harbin, 150030, Heilongjiang, China
PLoS ONE 11:e0158074. 2016..3% DMSO treatment did not affect expression of genes involved in spindle organization (Bub1 and Mad2) and apoptosis (NF-ÎşB, Pten, Bcl2, Caspase3 and Caspase9), however, it significantly decreased expression levels ..
- Inhibition of CDK7 bypasses spindle assembly checkpoint via premature cyclin B degradation during oocyte meiosisHaiyang Wang
Department of Animal Sciences, Chungbuk National University, Cheongju, Republic of Korea
Biochim Biophys Acta 1863:2993-3000. 2016..Notably, this acceleration occurred in the presence of SAC proteins including Mad2 and Bub3 at the kinetochores...
- Generation of a Spindle Checkpoint Arrest from Synthetic Signaling AssembliesIvan Yuan
Wellcome Trust Centre for Cell Biology, University of Edinburgh King s Buildings, Max Born Crescent, Edinburgh EH9 3BF, UK
Curr Biol . 2016..This leads to dynamic kinetochore recruitment of Mad proteins [8, 9], a conformational change in Mad2 [10-12], and formation of the mitotic checkpoint complex (MCC: Cdc20-Mad3-Mad2 [13-15])...
- The spindle checkpoint of budding yeast depends on a tight complex between the Mad1 and Mad2 proteinsR H Chen
Department of Physiology, University of California, San Francisco, San Francisco, California 94143 0444, USA
Mol Biol Cell 10:2607-18. 1999..We have cloned the MAD2 gene, which encodes a protein of 196 amino acids that remains at a constant level during the cell cycle...
- The interaction between Ran and NTF2 is required for cell cycle progressionB B Quimby
Department of Biochemistry, Emory University School of Medicine, Atlanta, GA 30322, USA
Mol Biol Cell 11:2617-29. 2000..Moreover, when Ran is inefficiently imported into the nucleus, cells arrest in G(2) in a MAD2 checkpoint-dependent manner...
- Inhibition of Cdh1-APC by the MAD2-related protein MAD2L2: a novel mechanism for regulating Cdh1C M Pfleger
Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02115, USA
Genes Dev 15:1759-64. 2001..Cdc20-APC is regulated through inhibition by the spindle assembly checkpoint protein MAD2. Knowledge of Cdh1-APC regulation is limited to the phosphorylation-dependent dissociation of Cdh1 from APC...
- Kinetochore Function and Cell Cycle Progression RevisionKatsumi Kitagawa; Fiscal Year: 2012..Our synthetic-lethality screen using a mad2-deletion mutant against a set of yeast deletion mutations identified 32 genes, including those encoding previously ..
- CELL AND TUMOR GENETICS OF MITOTIC ARREST CHECKPOINTSPETER KARL SORGER; Fiscal Year: 2010..will be analyzed in vitro and in vivo (2) The mechanism of action of a recently discovered negative regulator of Mad2 (CMT2) will be analyzed in detail to uncover key aspects of Mad2-mediated checkpoint signaling...
- Structure and Function of Spindle Checkpoint ProteinsXuelian Luo; Fiscal Year: 2012..The spindle checkpoint protein Mad2 binds to Cdc20, the mitotic activator of APC/C, and inhibits APC/CCdc20, thus delaying the onset of anaphase...
- Role of Mitotic Checkpoint Defects in Mouse Tumor ModelsROBERT I BENEZRA; Fiscal Year: 2010The mitotic checkpoint gene Mad2 has been shown in metazoans to be an important part of the mitotic checkpoint apparatus through biochemical and genetic experiments...
- KEVIN DANIEL CORBETT; Fiscal Year: 2016..the roles of Hop1's conserved HORMA domain, a signaling domain shared with the spindle checkpoint protein Mad2, in regulating inter-homolog meiotic recombination, and in a meiosis-specific checkpoint monitoring recombination...
- James E Haber; Fiscal Year: 2016..between the DNA damage checkpoint and the Spindle Assembly Checkpoint, following up our findings that deleting Mad2 shortens checkpoint arrest in wild type cells and suppresses the permanent arrest of adaptation-defective mutations ..
- Quantitative Measurements and Modeling of the Mitotic CheckpointJagesh V Shah; Fiscal Year: 2011..Recent observations are now converging on a model whereby genes, particularly in the kinetochore-mediated Mad2 signaling pathway, are not necessarily mutated directly, but that the expression levels of genes and thereby ..
- MITOTIC CHECKPOINT GENE MAD2 IN VERTEBRATESRobert Benezra; Fiscal Year: 2004The mitotic checkpoint gene Mad2 has been shown in metazoans to be an important part of the mitotic checkpoint apparatus through biochemical experiments...
- Analysis of Loss of Function of MAD2 in MammalsLoren Michel; Fiscal Year: 2006..have recently shown in both primary murine embryonic fibroblasts and human tumor cells that loss of one allele of MAD2, a mitotic checkpoint gene, is sufficient to cause chromosome instability...
- Metagenomic profiling of oral polymicrobial flora in head and neck cancersCharis Eng; Fiscal Year: 2011..epigenetic alterations of target tumor suppressor genes in HNSCC;(3) To functionally validate the involvement of MAD2 and/or MDR1 in HNSCC tumor suppression...
- Wen Hwa Lee; Fiscal Year: 2014..been increasingly recognized as a key regulator in mitotic processes including kinetochore-microtubule attachment, Mad2-dependent spindle assembly checkpoint activation and spindle assembly...
- Mechanism of Mitotic Checkpoint in Mammalian CellsTIMOTHY YEN; Fiscal Year: 2007..hBUB 1 specifies the assembly of subdomain of the kinetochore that consists of checkpoint proteins hBUBR1, MAD1, MAD2 and Cdc20...
- John Pierce Wise; Fiscal Year: 2016..cell division causing centrosome amplification focusing on Mps1 and Nek2A;3) Determine the role of Nek2A, Mad1 and Mad2 interactions in particulate Cr(VI)-induced spindle assembly checkpoint bypass;and 4) Identify those cellular and ..
- Mechanism of the Spindle Assembly Checkpoint ControlGuowei Fang; Fiscal Year: 2006..The activity of APC at metaphase is controlled by its activator CDC20 and by its inhibitor MAD2, a checkpoint protein...
- REGULATION OF THE ONSET OF ANAPHASETomohiro Matsumoto; Fiscal Year: 2001..The goal of this proposal is to elucidate the function of two genes, s1p1+ and mad2+, both of which play key roles in regulating the onset of anaphase to insure accurate chromosome segregation...
- S WALTER WALTER ENGLANDER; Fiscal Year: 2016..Study the role of transient partially unfolded states in other protein functions, with initial work directed at the Mad2 mitotic checkpoint protein...
- YsrRS regulon of Y. enterocoliticaVIRGINIA MILLER; Fiscal Year: 2009..enterocolitica strains. The Ysa TTSS is most closely related to the SPI-1 TTSS of Salmonella and Mxi-Spa TTSS of Shigella...
- IMMEDIATE-EARLY REGULATORY GENES OF CYTOMEGALOVIRUSGary Hayward; Fiscal Year: 2007..of post-transcriptional interactions of IE2 with four potential cell cycle regulatory proteins p21, JAB1, APC2 and MAD2; (II) continued exploration of the role of PML, DAXX, SUMO and the PODs at very early times in the nucleus, ..
- DIVERSE NATURAL PRODUCTS THAT STABILIZE MICROTUBULESSusan Horwitz; Fiscal Year: 2006..HeLa cells will be transfected with MAD2, a checkpoint component, to determine if its overexpression can rescue from cell death, those cells that escape ..
- HMG-1/Y AND NEOPLASTIC TRANSFORMATIONLinda Resar; Fiscal Year: 2002..4) Explore the role of Mad, Mxi, and AP-2 in the regulation of HMG-I/Y...
- Functional Analysis of Mitotic Checkpoint Gene Bub1bRakesh Goorha; Fiscal Year: 2005..For example, haplo-insufficiency of mitotic checkpoint gene Mad2 causes chromosome missegregation leading to aneuploidy in mammalian cells and the development of lung tumors in ..
- FAT10 a candidate gene for epithelial apoptosis in HIV-associated nephropathyMichael Ross; Fiscal Year: 2007..determine whether these SNPs alter the ability of FAT10 to associate with the mitotic spindle checkpoint protein MAD2. These studies will advance our understanding of HIVAN pathogenesis and may yield a novel therapeutic target for ..
- Mitotic Checkpoint & Genomic Stability in Ovarian CancerDong Yan Jin; Fiscal Year: 2006..proteins MAD1 and CDH1; (b) study the transcriptional regulation of the expression of checkpoint proteins MAD1 and MAD2; (c) characterize the interaction of MAD1-MAD2 with the nuclear pore complex; and (d) identify the upstream signals ..
- The Mechanism of Lung Chemoprevention Mediated by ITCsYang Ming Yang; Fiscal Year: 2005..The presence of BubR1 at kinetochores greatly facilitates the binding of Bub3, Mad1, Mad2 and CENP-E, so BubR1 may integrate the signals for spindle check point regulation...
- MYC NETWORK FUNCTION IN THE DEVELOPMENT OF CANCERLeila Alland; Fiscal Year: 1999..This interacting network of bHLH/LZ proteins consists of Myc,Max,Mad,and Mxi1. In addition to being an obligate partner in the transcriptionally competent Myc/Max complex,Max also appears to ..
- Analysis of Cdc20-Mad2 in spindle checkpoint signallingCLINTON COPELAND; Fiscal Year: 2006It has been well-documented that Mad1, Mad2, and Cdc20 have important roles in the spindle checkpoint in mitosis. In mammalian cells, Mad1 is responsible for Mad2 localization at the kinetochore...
- C-MYC REARRANGEMENTS IN HEMATOPOIETIC NEOPLASIASRiccardo Dalla Favera; Fiscal Year: 2000..Using this approach, we have identified two novel c-Myc target genes: I) Mxi which codes for a transcription factor which antagonizes the function of c-Myc by heterodimerizing with Max...
- Role of Alternative Mxi1 Isoforms in the Myc NetworkDaniel Wechsler; Fiscal Year: 2004..Through these studies, we will gain a better understanding of the role of these Mxii isoforms in the complex Myc signaling pathways involved in cell growth regulation and neoplasia. ..
- REGULATION OF MYC NETWORK FUNCTION IN DEVELOPMENTRonald DePinho; Fiscal Year: 1999..of Myc are highly dependent upon its dimerization with Max and are opposed by two other bHLH/LZ proteins, Mad and Mxi1. The contrasting biochemical properties of Myc and Mad or Mxi1 have led to a working model for the regulation of ..
- 800 MHZ NMR SPECTROMETERJOSE RIZO REY; Fiscal Year: 2002..structure and dynamics induced by natural and non-natural ligands; competition for the mitotic checkpoint protein Mad2 by its upstream regulator, Mad1, and its downstream target, Cdc20...
- Protection of Centromeric Cohesion by Bub1 and Sgo1Hongtao Yu; Fiscal Year: 2010....
- Regulation of the Anaphase-Promoting Complex by the Spindle CheckpointHongtao Yu; Fiscal Year: 2010..MCC contains BubR1, Bub3, Cdc20, and Mad2. BubR1 and Bub3 bind to each other constitutively throughout the cell cycle whereas the BubR1-Cdc20 and Mad2-Cdc20 ..
- Role of Nuf2 in Kinetochore FunctionJennifer DeLuca; Fiscal Year: 2004..abstract_text> ..
- SPINDLE ASSEMBLY CHECKPOINT IN VERTEBRATES AND YEASTRey Huei Chen; Fiscal Year: 2003..In addition to the biochemical studies, yeast genetics will be used to analyze Mad2 in detail and to identify other spindle assembly checkpoint components by suppressor screens...
- S6 Phosphorylation & Tyrosine Protein KinasesJAMES MALLER; Fiscal Year: 2004..Experiments will evaluate whether Bub1 mediates CSF arrest by the MAPK pathway. This work provides a link between the MAPK/Rsk pathway and checkpoint control of the cell cycle at the metaphase/anaphase transition. ..
- NEGATIVE REGULATION OF CELL CYCLE PROGRESSIONM Hoyt; Fiscal Year: 2004..The identified genes will report checkpoint activation and will allow a powerful dissection of this signal transduction pathway. ..
- CONTROL OF CELL DIVISION IN XENOPUS OOCYTESJAMES MALLER; Fiscal Year: 2003..Further work will examine the regulation, localization, and targets of PIx1 and identify kinases that activate xPIkk1. ..
- FUNCTION OF A DUAL SPECIFICITY TRANSCRIPTION FACTORPeter Hurlin; Fiscal Year: 2003..Finally, gene targeting in the mouse will be used to study the biological activities of Mga and the individual roles of its bHLHZip domain and T-domain. ..
- SHARED HIGH RESOLUTION MULTIMODE MICROSCOPE SYSTEMGreenfield Sluder; Fiscal Year: 2001..The proposed instrument system will allow these researchers to take their investigations in new directions, obtain novel information, and thus, significantly advance their programs in basic biomedical research. ..
- ANALYSIS AND CONTROL OF CHROMOSOME MOVEMENTR Nicklas; Fiscal Year: 2005..Kinetochores will be reconstructed by threedimensional tomography. The ultimate goal is to understand how tension, by altering structure, can lead to chemical changes such as dephosphorylation. ..
- Preventive of Bile Acid-Induced Colonic Oxidative DNA DamageClaire Margaret Payne; Fiscal Year: 2010..129 mice) developed in our laboratory;2) NOS-/- k.o./DOC mouse model;3) arginine-enhanced colon tumor ApcMin/+ mouse model developed in our laboratory;4) the AOM mouse model of colon carcinogenesis using A/J mice. ..
- Myc Signaling in MedulloblastomasCharles Eberhart; Fiscal Year: 2006..Finally, in Specific Aim 4 we propose developing a novel medulloblastoma transgenic model by overexpressing c-Myc in the cerebellum of transgenic mice. ..
- PREVENTION OF COLORECTAL CANCER BY iNOS AND COX-2 SELECTIVE INHIBITORSCHINTHALAPALLY RAO; Fiscal Year: 2008..abstract_text> ..
- Myc Antagonism and Tumor Suppression by MntPeter Hurlin; Fiscal Year: 2008..abstract_text> ..
- Cell Cycle Genes and Female Reproductive FunctionHiroaki Kiyokawa; Fiscal Year: 2008..No available cell line would generate such information. It is our expectation that this program contributes to a scientific foundation for therapeutic targets against infertility and other ovarian diseases. ..
- Regulation of the von Hippel-Lindau ProteinPaul Corn; Fiscal Year: 2007..abstract_text> ..
- Structure and function of the Mad2 checkpoint proteinXuelian Luo; Fiscal Year: 2007..During her mentored training period, she will continue to study the structure and function of the Mad2 spindle checkpoint protein...
- MECHANISMS FOR CHEMOPREVENTION OF COLON CANCERCHINTHALAPALLY RAO; Fiscal Year: 2004..Tissue distribution and comparative metabolism studies of curcumin and PEMC will be studied with synthetic [3H] curcumin and [3H]-PEMC in vivo in male F-344 rats. ..
- CHEMOKINES/CYTOKINES AND RECEPTORS IN STEM CELL HOMINGHal Broxmeyer; Fiscal Year: 2005..These studies should clarity the relevance of SDF-1 and CXCR4 for homing/engraftment and mobilization of primary stem/progenitor cells. ..
- Cytochrome C Oxidase I:A Biomarker for Colon Cancer RiskClaire Payne; Fiscal Year: 2006..Various molecular and cellular techniques will be used in these studies. ..
- Transcriptional Repression by the Myc Antagonist MxilNicole Schreiber Agus; Fiscal Year: 2006unreadable] DESCRIPTION (provided by applicant): The Mxi1 protein (and other members of the Mad/Mxi1 family) functions as a Myc antagonist and a growth/tumor suppressor, in part by effecting transcriptional repression of key target genes...
- Role of G1-Cdks in immortalization and oncogenesisHiroaki Kiyokawa; Fiscal Year: 2006..abstract_text> ..