MXI1

Summary

Gene Symbol: MXI1
Description: MAX interactor 1, dimerization protein
Alias: MAD2, MXD2, MXI, bHLHc11, max-interacting protein 1, MAX dimerization protein 2, Max-related transcription factor, class C basic helix-loop-helix protein 11
Species: human

Top Publications

  1. ncbi Mutation of the MXI1 gene in prostate cancer
    L R Eagle
    Department of Pediatrics, Children s Hospital of Pittsburgh, Pennsylvania 15213, USA
    Nat Genet 9:249-55. 1995
  2. ncbi An amino-terminal domain of Mxi1 mediates anti-Myc oncogenic activity and interacts with a homolog of the yeast transcriptional repressor SIN3
    N Schreiber-Agus
    Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, New York 10461
    Cell 80:777-86. 1995
  3. ncbi Localization of the human Mxi1 transcription factor gene (MXI1) to chromosome 10q24-q25
    D S Wechsler
    Department of Pediatrics, Johns Hopkins University School of Medicine, Baltimore, Maryland 21287
    Genomics 21:669-72. 1994
  4. ncbi Mapping of two genes encoding members of a distinct subfamily of MAX interacting proteins: MAD to human chromosome 2 and mouse chromosome 6, and MXI1 to human chromosome 10 and mouse chromosome 19
    S Edelhoff
    Department of Pathology, University of Washington, Seattle 98195
    Oncogene 9:665-8. 1994
  5. ncbi Mxi1, a protein that specifically interacts with Max to bind Myc-Max recognition sites
    A S Zervos
    Department of Genetics, Harvard Medical School, Boston, Massachusetts 02114
    Cell 72:223-32. 1993
  6. ncbi Role of Mxi1 in ageing organ systems and the regulation of normal and neoplastic growth
    N Schreiber-Agus
    Department of Molecular Genetics, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    Nature 393:483-7. 1998
  7. ncbi Commonly occurring loss and mutation of the MXI1 gene in prostate cancer
    E V Prochownik
    Section of Hematology Oncology, Children s Hospital of Pittsburgh, Pennsylvania 15213, USA
    Genes Chromosomes Cancer 22:295-304. 1998
  8. pmc Mxi1 inhibits the proliferation of U87 glioma cells through down-regulation of cyclin B1 gene expression
    I Manni
    Istituto Regina Elena, Centro Ricerca Sperimentale, Laboratorio di Oncogenesi Molecolare, Via delle Messi d Oro 156, 00158 Rome, Italy
    Br J Cancer 86:477-84. 2002
  9. doi miR-24-3p and miR-27a-3p promote cell proliferation in glioma cells via cooperative regulation of MXI1
    Weiyi Xu
    Key Laboratory of Gene Engineering of the Ministry of Education, State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat Sen University, Guangzhou, P R China
    Int J Oncol 42:757-66. 2013
  10. pmc Merotelic kinetochore orientation is a major mechanism of aneuploidy in mitotic mammalian tissue cells
    D Cimini
    Department of Biology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA
    J Cell Biol 153:517-27. 2001

Research Grants

  1. Kinetochore Function and Cell Cycle Progression Revision
    Katsumi Kitagawa; Fiscal Year: 2012
  2. CELL AND TUMOR GENETICS OF MITOTIC ARREST CHECKPOINTS
    PETER KARL SORGER; Fiscal Year: 2010
  3. Structure and Function of Spindle Checkpoint Proteins
    Xuelian Luo; Fiscal Year: 2012
  4. Role of Mitotic Checkpoint Defects in Mouse Tumor Models
    ROBERT I BENEZRA; Fiscal Year: 2010
  5. KEVIN DANIEL CORBETT; Fiscal Year: 2016
  6. James E Haber; Fiscal Year: 2016
  7. Quantitative Measurements and Modeling of the Mitotic Checkpoint
    Jagesh V Shah; Fiscal Year: 2011
  8. MITOTIC CHECKPOINT GENE MAD2 IN VERTEBRATES
    Robert Benezra; Fiscal Year: 2004
  9. Analysis of Loss of Function of MAD2 in Mammals
    Loren Michel; Fiscal Year: 2006
  10. Metagenomic profiling of oral polymicrobial flora in head and neck cancers
    Charis Eng; Fiscal Year: 2011

Detail Information

Publications240 found, 100 shown here

  1. ncbi Mutation of the MXI1 gene in prostate cancer
    L R Eagle
    Department of Pediatrics, Children s Hospital of Pittsburgh, Pennsylvania 15213, USA
    Nat Genet 9:249-55. 1995
    The Mxi1 protein negatively regulates Myc oncoprotein activity and thus potentially serves a tumour suppressor function. MXI1 maps to chromosome 10q24-q25, a region that is deleted in some cases of prostate cancer...
  2. ncbi An amino-terminal domain of Mxi1 mediates anti-Myc oncogenic activity and interacts with a homolog of the yeast transcriptional repressor SIN3
    N Schreiber-Agus
    Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, New York 10461
    Cell 80:777-86. 1995
    ..of Myc-responsive genes in which transactivation-competent Myc-Max heterodimers are opposed by repressive Mxi1-Max or Mad-Max complexes...
  3. ncbi Localization of the human Mxi1 transcription factor gene (MXI1) to chromosome 10q24-q25
    D S Wechsler
    Department of Pediatrics, Johns Hopkins University School of Medicine, Baltimore, Maryland 21287
    Genomics 21:669-72. 1994
  4. ncbi Mapping of two genes encoding members of a distinct subfamily of MAX interacting proteins: MAD to human chromosome 2 and mouse chromosome 6, and MXI1 to human chromosome 10 and mouse chromosome 19
    S Edelhoff
    Department of Pathology, University of Washington, Seattle 98195
    Oncogene 9:665-8. 1994
    Both the MAD and the MXI1 genes encode basic-helix-loop-helix-leucine zipper (bHLH-Zip) transcription factors which bind Max in vitro, forming a sequence-specific DNA-binding complex similar to the Myc-Max heterodimer...
  5. ncbi Mxi1, a protein that specifically interacts with Max to bind Myc-Max recognition sites
    A S Zervos
    Department of Genetics, Harvard Medical School, Boston, Massachusetts 02114
    Cell 72:223-32. 1993
    We used the interaction trap to isolate a novel human protein that specifically interacts with Max. This protein, Mxi1 (for Max interactor 1), contains a bHLH-Zip motif that is similar to that found in Myc family proteins...
  6. ncbi Role of Mxi1 in ageing organ systems and the regulation of normal and neoplastic growth
    N Schreiber-Agus
    Department of Molecular Genetics, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    Nature 393:483-7. 1998
    b>Mxi1 belongs to the Mad (Mxi1) family of proteins, which function as potent antagonists of Myc oncoproteins...
  7. ncbi Commonly occurring loss and mutation of the MXI1 gene in prostate cancer
    E V Prochownik
    Section of Hematology Oncology, Children s Hospital of Pittsburgh, Pennsylvania 15213, USA
    Genes Chromosomes Cancer 22:295-304. 1998
    ..We previously demonstrated that the MXI1 gene maps to 10q24-q25 and is mutated in some tumors with cytogenetically detectable deletions of this locus...
  8. pmc Mxi1 inhibits the proliferation of U87 glioma cells through down-regulation of cyclin B1 gene expression
    I Manni
    Istituto Regina Elena, Centro Ricerca Sperimentale, Laboratorio di Oncogenesi Molecolare, Via delle Messi d Oro 156, 00158 Rome, Italy
    Br J Cancer 86:477-84. 2002
    b>Mxi1 is a Mad family member that plays a role in cell proliferation and differentiation. To test the role of Mxi1 on tumorigenesis of glioma cells we transfected a CMV-driven MXI1 cDNA in U87 human glioblastoma cells...
  9. doi miR-24-3p and miR-27a-3p promote cell proliferation in glioma cells via cooperative regulation of MXI1
    Weiyi Xu
    Key Laboratory of Gene Engineering of the Ministry of Education, State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat Sen University, Guangzhou, P R China
    Int J Oncol 42:757-66. 2013
    ..By integrated bioinformatic analysis and experimental confirmation, we identified MXI1, which has been found to act as a tumor suppressor gene by affecting c‑Myc, as a direct target of miRâ..
  10. pmc Merotelic kinetochore orientation is a major mechanism of aneuploidy in mitotic mammalian tissue cells
    D Cimini
    Department of Biology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA
    J Cell Biol 153:517-27. 2001
    ..They also had inactivated mitotic spindle checkpoint activities since they did not label with either Mad2 or 3F3/2 antibodies...
  11. ncbi Influence of follicular fluid meiosis-activating sterol on aneuploidy rate and precocious chromatid segregation in aged mouse oocytes
    S Cukurcam
    Research Laboratories of Schering AG, Berlin, Germany
    Hum Reprod 22:815-28. 2007
    ..Reduced expression of cohesion and checkpoint proteins and predivision has been hypothesized to occur in age-related aneuploidy in oocytes...
  12. ncbi The first mitosis of the mouse embryo is prolonged by transitional metaphase arrest
    Marta Sikora-Polaczek
    Department of Embryology, Institute of Zoology, Warsaw University, 02 096 Warsaw, Poland
    Biol Reprod 74:734-43. 2006
    ..The end of prometaphase is correlated with checkpoint inactivation and disappearance of MAD2L1 (MAD2) and RSN (CLIP-170) proteins from kinetochores...
  13. ncbi The role of pre- and post-anaphase microtubules in the cytokinesis phase of the cell cycle
    J C Canman
    Department of Biology, University of North Carolina Chapel Hill, North Carolina 27599, USA
    Curr Biol 10:611-4. 2000
    ..Here, we have taken advantage of the discovery that microinjection of antibodies to the spindle checkpoint protein Mad2 (mitotic arrest deficient) in prometaphase abrogates the spindle checkpoint, producing premature chromosome ..
  14. pmc Control of chromosome stability by the beta-TrCP-REST-Mad2 axis
    Daniele Guardavaccaro
    Department of Pathology, NYU Cancer Institute, New York University School of Medicine, 550 First Avenue, MSB 599, New York, New York 10016, USA
    Nature 452:365-9. 2008
    ..the ubiquitin ligase SCF(beta-TrCP) during the G2 phase of the cell cycle to allow transcriptional derepression of Mad2, an essential component of the spindle assembly checkpoint...
  15. pmc Mlp1 acts as a mitotic scaffold to spatially regulate spindle assembly checkpoint proteins in Aspergillus nidulans
    Colin P De Souza
    Department of Molecular Genetics, Ohio State University, Columbus, 43210, USA
    Mol Biol Cell 20:2146-59. 2009
    ..In interphase, An-Mlp1 tethers the An-Mad1 and An-Mad2 SAC proteins to NPCs...
  16. pmc Ribonuclease activity of Dis3 is required for mitotic progression and provides a possible link between heterochromatin and kinetochore function
    Hiroaki Murakami
    CREST Research Program, Japan Science and Technology Corporation, Department of Gene Mechanisms, Graduate School of Biostudies, Kyoto University, Yoshida Honmachi, Sakyo ku, Kyoto, Japan
    PLoS ONE 2:e317. 2007
    ..Previous isolation of the dis3-54 cold-sensitive mutant of fission yeast Schizosaccharomyces pombe suggested that Dis3 is also required for correct chromosome segregation...
  17. ncbi Molecular and cellular mechanisms of invasion of the intestinal barrier by enteric pathogens. The paradigm of Shigella
    P J Sansonetti
    Unite de Pathogenie Microbienne Moleculaire, INSERM U 389, Institut Pasteur, Paris, France
    Folia Microbiol (Praha) 43:239-46. 1998
    ..e. invasins) through a specialized, activable, type-III secretory apparatus (i.e. Mxi/Spa)...
  18. pmc Visualization of Mad2 dynamics at kinetochores, along spindle fibers, and at spindle poles in living cells
    B J Howell
    Department of Biology, CB 3280, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA
    J Cell Biol 150:1233-50. 2000
    ..A key checkpoint component is the mitotic arrest-deficient protein 2 (Mad2), which localizes to unattached kinetochores and inhibits activation of the anaphase-promoting complex (APC) ..
  19. ncbi Mad2 is required for inhibiting securin and cyclin B degradation following spindle depolymerisation in meiosis I mouse oocytes
    Hayden A Homer
    Newcastle Fertility Centre at Life, International Centre for Life, Times Square, Newcastle upon Tyne, NE1 4EP, UK
    Reproduction 130:829-43. 2005
    b>Mad2 is a pivotal component of the spindle assembly checkpoint (SAC) which inhibits anaphase promoting complex/cyclo-some (APC/C) activity by sequestering Cdc20 thereby regulating the destruction of securin and cyclin B...
  20. ncbi Cell cycle-dependent interaction of Mad2 with conserved Box1/2 region of human granulocyte-macrophage colony-stimulating factor receptor common betac
    M Takeda
    Department of Molecular and Developmental Biology, Institute of Medical Science, Core Research for Evolutional Science and Technology, Japan
    J Biol Chem 276:41803-9. 2001
    ..The mouse homologue of Mad2 protein, which plays an important role in the M phase of the cell cycle, was revealed to associate with the box1/2 ..
  21. ncbi Anaphase onset does not require the microtubule-dependent depletion of kinetochore and centromere-binding proteins
    Julie C Canman
    University of North Carolina, Department of Biology, 607 Fordham Hall, CB3280, Chapel Hill, NC 27599, USA
    J Cell Sci 115:3787-95. 2002
    Spindle checkpoint proteins, such as Mad2 and BubR1, and the motors dynein/dynactin and CENP-E usually leave kinetochores prior to anaphase onset by microtubule-dependent mechanisms...
  22. pmc Smurf2 as a novel mitotic regulator: From the spindle assembly checkpoint to tumorigenesis
    Evan C Osmundson
    Department of Molecular Pharmacology and Biological Chemistry, Northwestern University Feinberg School of Medicine, 303 E, Chicago Avenue, Chicago, IL 60611, USA
    Cell Div 4:14. 2009
    ..The critical effector of the spindle checkpoint, Mitotic arrest deficient 2 (Mad2), is recruited to unattached kinetochores forming a complex with other regulatory proteins to efficiently and ..
  23. pmc The corepressor N-CoR and its variants RIP13a and RIP13Delta1 directly interact with the basal transcription factors TFIIB, TAFII32 and TAFII70
    G E Muscat
    University of Queensland, Centre for Molecular and Cellular Biology, Ritchie Research Laboratories, B402A, St Lucia 4072, Queensland, Australia
    Nucleic Acids Res 26:2899-907. 1998
    ..g. TR, RAR), the orphan nuclear receptors (e.g. Rev-erbAalpha/beta), Mxi-1 and Mad bHLH-zip proteins and the oncoproteins PLZF and LAZ3/BCL6 is mediated by the corepressors N-CoR and SMRT...
  24. pmc Mammalian p55CDC mediates association of the spindle checkpoint protein Mad2 with the cyclosome/anaphase-promoting complex, and is involved in regulating anaphase onset and late mitotic events
    M Kallio
    Department of Cell Biology, Health Sciences Center, University of Virginia, Charlottesville, Virginia 22908, USA
    J Cell Biol 141:1393-406. 1998
    ..p55CDC coimmunoprecipitates with three important elements of the M phase checkpoint machinery: Cdc27, Cdc16, and Mad2. p55CDC is required for binding Mad2 with the Cdc27 and Cdc16...
  25. pmc Determinants of conformational dimerization of Mad2 and its inhibition by p31comet
    Marina Mapelli
    Department of Experimental Oncology, European Institute of Oncology, Milan, Italy
    EMBO J 25:1273-84. 2006
    ..The ubiquitous SAC constituents Mad1 and Mad2 are recruited to kinetochores in prometaphase...
  26. pmc Changing Mad2 levels affects chromosome segregation and spindle assembly checkpoint control in female mouse meiosis I
    Théodora Niault
    CNRS UMR7622 Biologie du DĂ©veloppement, Paris, France
    PLoS ONE 2:e1165. 2007
    ..Prolonged metaphase arrest due to overexpression of Mad2 has been shown to be deleterious to the ensuing anaphase, leading to the generation of aneuploidies and ..
  27. ncbi FAT10 plays a role in the regulation of chromosomal stability
    Jianwei Ren
    Division of Medical Sciences, National Cancer Center, Singapore 169610, Singapore
    J Biol Chem 281:11413-21. 2006
    ..Here we show that FAT10 interacts with MAD2, a spindle checkpoint protein, during mitosis...
  28. pmc Cytoplasmic dynein/dynactin drives kinetochore protein transport to the spindle poles and has a role in mitotic spindle checkpoint inactivation
    B J Howell
    Department of Biology, University of North Carolina, Chapel Hill, NC 27599, USA
    J Cell Biol 155:1159-72. 2001
    ..the microtubule motors CENP-E and cytoplasmic dynein, and proteins involved with the mitotic spindle checkpoint, Mad2, Bub1R, and the 3F3/2 phosphoantigen...
  29. ncbi Differences in spindle association of the mitotic checkpoint protein Mad2 in mammalian spermatogenesis and oogenesis
    M Kallio
    Turku Center for Biotechnology, BioCity, 5th Floor, Tykistökatu 6B, Turku, FIN 20520, Finland
    Dev Biol 225:112-23. 2000
    We have investigated expression and subcellular localization of the spindle checkpoint protein Mad2 during rat and mouse spermatogenesis and in superovulated mouse oocytes...
  30. pmc Novel mad2 alleles isolated in a Schizosaccharomyces pombe gamma-tubulin mutant are defective in metaphase arrest activity, but remain functional for chromosome stability in unperturbed mitosis
    Yoshie Tange
    Kazusa DNA Research Institute, Kisarazu, Chiba 292 0818, Japan
    Genetics 175:1571-84. 2007
    ..In the absence of the spindle assembly checkpoint gene, mad2+, the spindle dynamics in the gamma-tubulin mutant were greatly compromised, leading to a high incidence of ..
  31. pmc Mad2 and BubR1 function in a single checkpoint pathway that responds to a loss of tension
    Katie B Shannon
    SPIRE fellow, Department of Biology, University of North Carolina, Chapel Hill, North Carolina 27599 3280, USA
    Mol Biol Cell 13:3706-19. 2002
    ..The metaphase delay at 23 degrees C was abrogated by injection of Mad2 inhibitors, showing that Mad2 and the spindle checkpoint were responsible for the prolonged metaphase...
  32. pmc Mad2-independent spindle assembly checkpoint activation and controlled metaphase-anaphase transition in Drosophila S2 cells
    Bernardo Orr
    Instituto de Biologia Molecular e Celular, 4150 180 Porto, Portugal
    Mol Biol Cell 18:850-63. 2007
    ..We analyzed the role of the putative Drosophila Mad2 homologue in the spindle assembly checkpoint and mitotic progression...
  33. pmc Microinjection of antibody to Mad2 protein into mammalian cells in mitosis induces premature anaphase
    G J Gorbsky
    Department of Cell Biology, University of Virginia, Charlottesville, Virginia 22908, USA
    J Cell Biol 141:1193-205. 1998
    In yeast, the Mad2 protein is required for the M phase arrest induced by microtubule inhibitors, but the protein is not essential under normal culture conditions...
  34. pmc Checkpoint protein BubR1 acts synergistically with Mad2 to inhibit anaphase-promoting complex
    Guowei Fang
    Department of Biological Sciences, Stanford University, Stanford, California 94305 5020, USA
    Mol Biol Cell 13:755-66. 2002
    ..In response to activation of the checkpoint, Mad2 binds to and inhibits Cdc20-APC...
  35. pmc MxiE regulates intracellular expression of factors secreted by the Shigella flexneri 2a type III secretion system
    Colleen D Kane
    Department of Microbiology and Immunology, F Edward Hebert School of Medicine, Uniformed Services University of the Health Sciences, Bethesda, Maryland 20814 4799, USA
    J Bacteriol 184:4409-19. 2002
    The mxi-spa locus on the virulence plasmid of Shigella flexneri encodes components of the type III secretion system...
  36. ncbi An Inv/Mxi-Spa-like type III protein secretion system in Burkholderia pseudomallei modulates intracellular behaviour of the pathogen
    Mark P Stevens
    Division of Environmental Microbiology, Institute for Animal Health, Compton Laboratory, Berkshire RG20 7NN, UK
    Mol Microbiol 46:649-59. 2002
    ..pseudomallei and host cells. The Bsa system is very similar to the Inv/Mxi-Spa type III secretion systems of Salmonella and Shigella. Moreover, B...
  37. ncbi Nuf2 and Hec1 are required for retention of the checkpoint proteins Mad1 and Mad2 to kinetochores
    Jennifer G DeLuca
    Department of Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
    Curr Biol 13:2103-9. 2003
    ..results in a strong prometaphase block with an active spindle checkpoint, which correlates with low but detectable Mad2 at kinetochores that have no or few stable kinetochore microtubules...
  38. ncbi Attenuated virulence and protective efficacy of a Burkholderia pseudomallei bsa type III secretion mutant in murine models of melioidosis
    Mark P Stevens
    Division of Microbiology, Institute for Animal Health, Compton Laboratory, Berkshire RG20 7NN, UK
    Microbiology 150:2669-76. 2004
    ..An Inv/Mxi-Spa-like type III protein secretion apparatus, encoded by the B...
  39. pmc A Tel1/MRX-dependent checkpoint inhibits the metaphase-to-anaphase transition after UV irradiation in the absence of Mec1
    Michela Clerici
    Dipartimento di Biotecnologie e Bioscienze, Universita degli Studi di Milano Bicocca, P zza della Scienza 2, 20126 Milan, Italy
    Mol Cell Biol 24:10126-44. 2004
    ..The spindle assembly checkpoint protein Mad2 also partially contributes to the G(2)/M arrest of UV-irradiated mec1Delta cells independently of Rad53 ..
  40. ncbi Cytoplasmic dynein participates in meiotic checkpoint inactivation in mouse oocytes by transporting cytoplasmic mitotic arrest-deficient (Mad) proteins from kinetochores to spindle poles
    Dong Zhang
    State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100080, China
    Reproduction 133:685-95. 2007
    ..meiosis and its relationship with two major spindle checkpoint proteins, mitotic arrest-deficient (Mad) 1 and Mad2. Oocytes at various stages during the first meiosis were fixed and immunostained for dynein, Mad1, Mad2, ..
  41. pmc The spindle checkpoint functions of Mad3 and Mad2 depend on a Mad3 KEN box-mediated interaction with Cdc20-anaphase-promoting complex (APC/C)
    Matylda Sczaniecka
    Wellcome Trust Centre for Cell Biology, Institute of Cell Biology, University of Edinburgh, EH9 3JR, United Kingdom
    J Biol Chem 283:23039-47. 2008
    ..In the fission yeast, Schizosaccharomyces pombe, we find that the Mad3 and Mad2 spindle checkpoint proteins interact stably with the APC/C in mitosis...
  42. doi Epstein-Barr virus nuclear antigen 2 disrupts mitotic checkpoint and causes chromosomal instability
    Shih Hsuan Pan
    Graduate Institute of Microbiology, College of Medicine, National Taiwan University, Taipei 100, Taiwan
    Carcinogenesis 30:366-75. 2009
    ..EBNA2 was shown to be able to downregulate mitotic arrest deficient 2 (MAD2) approximately 2- to 3-fold and upregulate polo-like kinase 1 (PLK1) approximately 2-fold...
  43. pmc Unattached kinetochores catalyze production of an anaphase inhibitor that requires a Mad2 template to prime Cdc20 for BubR1 binding
    Anita Kulukian
    Ludwig Institute for Cancer Research, University of California at San Diego, La Jolla, CA 92093, USA
    Dev Cell 16:105-17. 2009
    ..Using physiologically relevant levels of Mad2, Bub3, BubR1, and Cdc20, we demonstrate that unattached kinetochores on purified chromosomes catalytically generate ..
  44. ncbi Checkpoint signals in grasshopper meiosis are sensitive to microtubule attachment, but tension is still essential
    R B Nicklas
    Department of Biology, Duke University, Durham, NC 27708 1000, USA
    J Cell Sci 114:4173-83. 2001
    ..The checkpoint is activated by attachment-sensitive components including Mad2 and certain phosphorylated proteins detected by the 3F3/2 antibody...
  45. ncbi Requirement of chromatid cohesion proteins rad21/scc1 and mis4/scc2 for normal spindle-kinetochore interaction in fission yeast
    Yusuke Toyoda
    Department of Gene Mechanisms, Graduate School of Biostudies, 606 8502, Kyoto, Japan
    Curr Biol 12:347-58. 2002
    ..The failure to form cohesion in the S phase results in premature separation of chromatids in G2/M. Mitotic kinetochores free from microtubules or the lack of tension are known to activate spindle checkpoint...
  46. ncbi Loss of heterozygosity and microsatellite instability on chromosome arm 10q in neuroblastoma
    Paula Lázcoz
    Department of Health Sciences, Public University of Navarra, 31006 Pamplona, Spain
    Cancer Genet Cytogenet 174:1-8. 2007
    ..We screened the 10q locus for loss of heterozygosity and the promoter methylation status of PTEN, MGMT, MXI1, and FGFR2 in neuroblastic tumors and neuroblastoma cell lines...
  47. pmc The HECT E3 ligase Smurf2 is required for Mad2-dependent spindle assembly checkpoint
    Evan C Osmundson
    Department of Molecular Pharmacology and Biological Chemistry, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USA
    J Cell Biol 183:267-77. 2008
    ..Smurf2 depletion results in enhanced polyubiquitination and degradation of Mad2, a critical checkpoint effector...
  48. pmc Induction of type III secretion in Shigella flexneri is associated with differential control of transcription of genes encoding secreted proteins
    B Demers
    Unité de Pathogénie Microbienne and Unité INSERM, Institut Pasteur, Paris, France
    EMBO J 17:2894-903. 1998
    ..Entry of Shigella into epithelial cells involves a type III secretion machinery, encoded by the mxi and spa operons, and the IpaA-D secreted proteins...
  49. ncbi Induction of max by adrenomedullin and calcitonin gene-related peptide antagonizes endothelial apoptosis
    M Shichiri
    Second Department of Internal Medicine, Tokyo Medical and Dental University, Japan
    Mol Endocrinol 13:1353-63. 1999
    ..is a basic-helix-loop-helix-leucine zipper protein that forms heterodimers with its alternative partners, Mad and Mxi-1, to behave as an antagonist for Myc-Max heterodimer through competition for common DNA targets...
  50. ncbi Control of localization of a spindle checkpoint protein, Mad2, in fission yeast
    Amy E Ikui
    Department of Radiation Oncology, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461, USA
    J Cell Sci 115:1603-10. 2002
    ..b>Mad2, a component of the checkpoint, targets fission yeast Slp1/budding yeast Cdc20/human p55CDC and prevents it from ..
  51. ncbi Mouse Sin3A interacts with and can functionally substitute for the amino-terminal repression of the Myc antagonist Mxi1
    G Rao
    Department of Cell Biology, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    Oncogene 12:1165-72. 1996
    b>Mxi1 is a basic region helix-loop-helix leucine zipper (bHLH/LZ) protein that, in association with Max, antagonizes Myc oncogenic activities...
  52. pmc Insights into mad2 regulation in the spindle checkpoint revealed by the crystal structure of the symmetric mad2 dimer
    Maojun Yang
    Department of Pharmacology, The University of Texas Southwestern Medical Center, Dallas, Texas, United States of America
    PLoS Biol 6:e50. 2008
    In response to misaligned sister chromatids during mitosis, the spindle checkpoint protein Mad2 inhibits the anaphase-promoting complex or cyclosome (APC/C) through binding to its mitotic activator Cdc20, thus delaying anaphase onset...
  53. doi Kinetic analysis of Mad2-Cdc20 formation: conformational changes in Mad2 are catalyzed by a C-Mad2-ligand complex
    Latesh Lad
    Cytokinetics Inc, South San Francisco, California 94080, USA
    Biochemistry 48:9503-15. 2009
    Structural changes in the mitotic arrest deficient protein 2 (Mad2) have been proposed to be essential for spindle checkpoint function...
  54. ncbi MAD2 downregulation in hypoxia is independent of promoter hypermethylation
    Maria Prencipe
    UCD School of Medicine and Medical Science SMMS, University College Dublin, Belfield, Dublin, Ireland
    Cell Cycle 9:2856-65. 2010
    Aberrant expression of the MAD2 protein has been linked to chromosomal instability, malignant transformation and chemoresistance...
  55. ncbi Assignment of the human MAD and MXI1 genes to chromosomes 2p12-p13 and 10q24-q25
    D N Shapiro
    Department of Experimental Oncology, St Jude Children s Research Hospital, Memphis, Tennessee 38101
    Genomics 23:282-5. 1994
    MAD and MXI1, two recently described members of the basic helix-loop-helix (bHLH) gene family, encode proteins that dimerize with and modulate the DNA binding of max...
  56. pmc The LAZ3(BCL-6) oncoprotein recruits a SMRT/mSIN3A/histone deacetylase containing complex to mediate transcriptional repression
    P Dhordain
    U124 INSERM IRCL, Place de Verdun, F 59045 Lille Cedex, France and Howard Hughes Medical Institute and The Salk Institute for Biological Studies, La Jolla, CA 92037, USA
    Nucleic Acids Res 26:4645-51. 1998
    ..For instance, the bHLH-Zip transcriptional repressors MAD/MXI recruit HDACs together with the mSIN3 corepressors, whereas unliganded nuclear receptors contact another ..
  57. ncbi IpgD, a protein secreted by the type III secretion machinery of Shigella flexneri, is chaperoned by IpgE and implicated in entry focus formation
    K Niebuhr
    Unite de Pathogenie Microbienne Moleculaire, INSERM U389, Institut Pasteur, 28 rue du Dr Roux, 75724 Paris Cedex 15, France
    Mol Microbiol 38:8-19. 2000
    ..Entry of bacteria into non-phagocytic cells requires the IpaA-D proteins that are secreted by the Mxi-Spa type III secretion machinery...
  58. ncbi Partially functional Cenpa-GFP fusion protein causes increased chromosome missegregation and apoptosis during mouse embryogenesis
    Paul Kalitsis
    Murdoch Childrens Research Institute, Royal Children s Hospital, Flemington Road, Parkville 3052, Melbourne, Victoria, Australia
    Chromosome Res 11:345-57. 2003
    ..that bind to a host of centromere-specific structural and mitotic spindle checkpoint proteins (Cenpc, BubR1, Mad2 and Zw10)...
  59. pmc Mad2 prevents aneuploidy and premature proteolysis of cyclin B and securin during meiosis I in mouse oocytes
    Hayden A Homer
    Newcastle Fertility Centre at Life, Centre for Life, Newcastle upon Tyne NE1 4EP, United Kingdom
    Genes Dev 19:202-7. 2005
    In mitosis, the spindle checkpoint protein Mad2 averts aneuploidy by delaying anaphase onset until chromosomes align. Here we show that depletion of Mad2 in meiosis I mouse oocytes induced an increased incidence of aneuploidy...
  60. ncbi Genomic models of metastatic cancer: functional analysis of death-from-cancer signature genes reveals aneuploid, anoikis-resistant, metastasis-enabling phenotype with altered cell cycle control and activated Polycomb Group (PcG) protein chromatin silencin
    Gennadi V Glinsky
    Functional Genomics Laboratory, Ordway Cancer Center, Ordway Research Institute, Inc, Center for Medical Sciences, Division of Urology, Albany Medical College, New York 12208 USA
    Cell Cycle 5:1208-16. 2006
    ..protein (IAP) family (Survivin and XIAP); activation of mitotic spindle check point proteins (BUB1, BUB3, KNTC2, Mad2, PLK1, PLK4, STK6/Aurora A); and elevated levels of certain cell cycle control/marker proteins (CCNB1, CCNB2, CCND1,..
  61. pmc In vivo analysis of chromosome condensation in Saccharomyces cerevisiae
    Amit C J Vas
    Department of Genetics, Cell Biology, and Development, University of Minnesota Medical School, Minneapolis, MN 55455, USA
    Mol Biol Cell 18:557-68. 2007
    ..progression by activation of the spindle assembly checkpoint caused a loss of condensation that was dependent on Mad2, followed by a delayed loss of cohesion between sister chromatids...
  62. ncbi Mechanisms of mitotic cell death induced by chemotherapy-mediated G2 checkpoint abrogation
    Celia Vogel
    Institute for Molecular Biology and Tumor Research, Philipps University of Marburg, Emil Mannkopff Strasse 2, D 35037 Marburg, Germany
    Cancer Res 67:339-45. 2007
    ..treatment results in a mitotic arrest that requires an active mitotic spindle checkpoint, involving the function of Mad2, Bub1, BubR1, Mps1, Aurora B, and survivin...
  63. pmc Two different mitotic checkpoint inhibitors of the anaphase-promoting complex/cyclosome antagonize the action of the activator Cdc20
    Esther Eytan
    Unit of Biochemistry, Rappaport Faculty of Medicine, Technion Israel Institute of Technology, Haifa 31096, Israel
    Proc Natl Acad Sci U S A 105:9181-5. 2008
    ..complex (MCC) inhibitor of APC/C composed of the APC/C activator Cdc20 associated with the checkpoint proteins Mad2, BubR1, and Bub3...
  64. doi Effects of heat stress during in vitro maturation on cytoplasmic versus nuclear components of mouse oocytes
    Jun Zuo Wang
    Laboratory for Animal Reproduction and Embryology, College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Shandong Province, People s Republic of China
    Reproduction 137:181-9. 2009
    ..While spindle assembling was normal and no MAD2 was activated in oocytes matured at 37 or 40 degrees C, spindle assembling was affected and MAD2 was activated in ..
  65. doi Methylation of p16INK4A and mitotic arrest defective protein 2 (MAD2) genes in gastric marginal-zone B-cell lymphomas
    Sanghui Park
    Department of Pathology, Gachon University of Medicine and Science, Gil Medical Center, Incheon, Republic of Korea
    Acta Haematol 120:217-24. 2008
    Products of cyclin-dependent kinase inhibitor p16(INK4A)and mitotic arrest defective protein 2 (MAD2) genesare key regulator proteins at the G1 restriction point and mitotic checkpoint of the cell cycle...
  66. pmc Dynein light intermediate chain 1 is required for progress through the spindle assembly checkpoint
    Mylavarapu V S Sivaram
    Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, MA 01605, USA
    EMBO J 28:902-14. 2009
    ..Metaphase delay and increased interkinetochore distances are suppressed by depletion of Mad1, Mad2 or BubR1 or by re-expression of wtLIC1 or a Cdk1 site phosphomimetic LIC1 mutant, but not Cdk1-phosphorylation-..
  67. doi Heat shock factor 1-mediated aneuploidy requires a defective function of p53
    Eun Ho Kim
    Division of Radiation Effects, Korea Institute of Radiological and Medical Sciences, Seoul, Korea
    Cancer Res 69:9404-12. 2009
    ..Moreover, the interaction affinity of Cdc20 with Mad2 was inhibited in p53Mut cells, whereas the interaction between Cdc20 and HSF1 was increased...
  68. pmc ATP is required for the release of the anaphase-promoting complex/cyclosome from inhibition by the mitotic checkpoint
    Shirly Miniowitz-Shemtov
    Unit of Biochemistry, Rappaport Faculty of Medicine, Technion Israel Institute of Technology, Haifa 31096, Israel
    Proc Natl Acad Sci U S A 107:5351-6. 2010
    ..APC/C is inhibited by a mitotic checkpoint complex (MCC) composed of BubR1, Bub3, Mad2, and Cdc20...
  69. pmc Small-molecule kinase inhibitors provide insight into Mps1 cell cycle function
    Nicholas Kwiatkowski
    Department of Cancer Biology, Dana Farber Cancer Institute, Boston, Massachusetts, USA
    Nat Chem Biol 6:359-68. 2010
    ..Consistent with RNAi studies, chemical inhibition of Mps1 leads to defects in Mad1 and Mad2 establishment at unattached kinetochores, decreased Aurora B kinase activity, premature mitotic exit and gross ..
  70. doi Replication-compromised cells require the mitotic checkpoint to prevent tetraploidization
    Zilai Zhang
    Department of Pathology, Stanford University Medical Center, Edwards Building, Room R270, 300 Pasteur Drive, Stanford, CA 94305 5324, USA
    Chromosoma 120:73-82. 2011
    ..the mutant Polα-protein failed to sustain mitotic arrest and, when propagated progressively, down-regulated Mad2 and BubR1 and accumulated 4N-DNA from the 2N-DNA cells...
  71. pmc DNA methylation profiling of medulloblastoma allows robust subclassification and improved outcome prediction using formalin-fixed biopsies
    Edward C Schwalbe
    Northern Institute for Cancer Research, Newcastle University, Sir James Spence Institute Level 5, Royal Victoria Infirmary, Newcastle upon Tyne, NE1 4LP, UK
    Acta Neuropathol 125:359-71. 2013
    ..b>MXI1 and IL8 methylation were identified as novel independent high-risk biomarkers in cross-validated survival models of ..
  72. doi A synthetic dolastatin 10 analogue suppresses microtubule dynamics, inhibits cell proliferation, and induces apoptotic cell death
    Praveen Kumar Gajula
    CSIR Central Drug Research Institute, Lucknow 226 001 India
    J Med Chem 56:2235-45. 2013
    ..D5 increased the accumulation of checkpoint proteins BubR1 and Mad2 at the kinetochoric region and caused G2/M block in these cells...
  73. doi MYC and MXI1 protein expression: potential prognostic significance in women with breast cancer in China
    Lin Ping Xu
    The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China
    Oncol Res Treat 37:118-23. 2014
    To investigate the expression levels and the clinical significance of MYC and MXI1 proteins in breast cancer.
  74. ncbi Mitotic checkpoint proteins Mad1 and Mad2 - structural and functional relationship with implication in genetic diseases
    Speranta Avram
    Anatomy, Animal Physiology and Biophysics Department, Faculty of Biology, University of Bucharest, 91 95th Independentei Str, Sector 5, Bucharest 050095, Romania
    Curr Comput Aided Drug Des 10:168-81. 2014
    ..deeply involved in correct cell divisions, but some of these, such as mitotic arrest-deficient proteins (Mad1 and Mad2), are critical...
  75. doi Spindle assembly checkpoint protein expression correlates with cellular proliferation and shorter time to recurrence in ovarian cancer
    Barbara McGrogan
    UCD School of Medicine and Medical Science, University College Dublin, UCD, Belfield, Dublin 4, Ireland UCD Conway Institute of Biomolecular and Biomedical Research, University College Dublin, Belfield, Dublin 4, Ireland National Cancer Control Programme, King s Inns House, Dublin 1, Ireland Electronic address
    Hum Pathol 45:1509-19. 2014
    ..BUB1-related protein kinase (BUBR1) and mitotic arrest deficient 2 (MAD2), essential spindle assembly checkpoint components, play a key role in response to taxanes...
  76. pmc MicroRNA 28 controls cell proliferation and is down-regulated in B-cell lymphomas
    Christof Schneider
    Institute for Cancer Genetics
    Proc Natl Acad Sci U S A 111:8185-90. 2014
    ..of miR-28 impairs cell proliferation and clonogenic properties of BL cells by modulating several targets including MAD2 mitotic arrest deficient-like 1, MAD2L1, a component of the spindle checkpoint whose down-regulation is essential ..
  77. pmc Stranglehold on the spindle assembly checkpoint: the human papillomavirus E2 protein provokes BUBR1-dependent aneuploidy
    Chye Ling Tan
    a Cell Cycle Control in Skin Epidermis Institute of Medical Biology A Star, Biopolis Immunos, Singapore
    Cell Cycle 14:1459-70. 2015
    ..This phenotype is associated with interaction of E2 with the Mitotic Checkpoint Complex (MCC) proteins Cdc20, MAD2 and BUBR1...
  78. pmc The Spindle Assembly Checkpoint Is Not Essential for Viability of Human Cells with Genetically Lowered APC/C Activity
    Thomas Wild
    Proteomics Program, The Novo Nordisk Foundation Center for Protein Research, Faculty of Health and Medical Sciences, University of Copenhagen, Blegdamsvej 3b, 2200 Copenhagen, Denmark
    Cell Rep 14:1829-40. 2016
    ..transition and, strikingly, renders cells insensitive to chemical inhibition of MPS1 and genetic ablation of MAD2, both of which are essential for the SAC...
  79. doi RING domain is essential for the antiviral activity of TRIM25 from orange spotted grouper
    Ying Yang
    Key Laboratory of Tropical Marine Bio resources and Ecology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, 164 West Xingang Road, Guangzhou 510301, China University of Chinese Academy of Sciences, Beijing, China
    Fish Shellfish Immunol 55:304-14. 2016
    ..signaling molecules, including interferon regulatory factor (IRF) 3, interferon-induced 35-kDa protein (IFP35), MXI, IRF7 and myeloid differentiation factor 88 (MyD88), suggesting that the positive regulation of interferon immune ..
  80. ncbi The Bfa1/Bub2 GAP complex comprises a universal checkpoint required to prevent mitotic exit
    Y Wang
    Verna and Mars McLean Department of Biochemistry and Molecular Biology, Howard Hughes Medical Institute, Baylor College of Medicine, Houston, Texas 77030, USA
    Curr Biol 10:1379-82. 2000
    ..cdc13-1 bub2 and cdc13-1 bfa1 but not cdc13-1 mad2 double mutants rebud and reduplicate their DNA at the restrictive temperature...
  81. ncbi Significance of MAD2 expression to mitotic checkpoint control in ovarian cancer cells
    Xianghong Wang
    Department of Anatomy, Faculty of Medicine, University of Hong Kong, Hong Kong
    Cancer Res 62:1662-8. 2002
    ..Mitotic checkpoint controls are thought to be essential for accurate chromosomal segregation, and MAD2 is a key component of this checkpoint...
  82. pmc Identification of a MAD2-binding protein, CMT2, and its role in mitosis
    Toshiyuki Habu
    Department of Radiation Oncology, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461, USA
    EMBO J 21:6419-28. 2002
    b>MAD2 is a key component of the spindle checkpoint that delays the onset of anaphase until all the kinetochores are attached to the spindle...
  83. pmc The role of Cdc55 in the spindle checkpoint is through regulation of mitotic exit in Saccharomyces cerevisiae
    Christopher M Yellman
    Department of Biochemistry and Molecular Genetics, University of Virginia Medical Center, University of Virginia, Charlottesville, VA 22908, USA
    Mol Biol Cell 17:658-66. 2006
    ..pathways, one leading to inhibition of the metaphase-to-anaphase transition by checkpoint proteins, including Mad2, and the other to inhibition of mitotic exit by Bub2. We show that Cdc55 is a negative regulator of mitotic exit...
  84. ncbi RNA Interference as a tool to study the function of MAD2 in mouse oocyte meiotic maturation
    Jian Ying Wang
    Key Laboratory of Animal Resistance Research, College of Life Science, Shandong Normal University, Jinan, PR China
    Mol Reprod Dev 74:116-24. 2007
    ..As a member of spindle checkpoint proteins, MAD2 takes a central role in the regulation of anaphase onset and genome integrity...
  85. ncbi A functional genomic screen identifies a role for TAO1 kinase in spindle-checkpoint signalling
    Viji M Draviam
    Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Nat Cell Biol 9:556-64. 2007
    ..TAO1 interacts with the checkpoint kinase BubR1 and promotes enrichment of the checkpoint protein Mad2 at sites of defective attachment, providing evidence for a regulatory step that precedes the proposed Mad2-Mad1 ..
  86. ncbi Spindle formation, chromosome segregation and the spindle checkpoint in mammalian oocytes and susceptibility to meiotic error
    E Vogt
    University of Bielefeld, Faculty of Biology, Gene Technology Microbiology, Bielefeld, Germany
    Mutat Res 651:14-29. 2008
    ..in the presence of unattached chromosomes, associated with accumulation of checkpoint components, in particular, Mad2, at the kinetochores...
  87. doi Prognostic significance of O6-methylguanine DNA methyltransferase and p57 methylation in patients with diffuse large B-cell lymphomas
    Sun Mi Lee
    Department of Pathology, The Catholic University of Korea, Seoul, Korea
    APMIS 117:87-94. 2009
    ..institute (n=44) were studied for methylation of tumor-related genes, MGMT, p15(INK4B), p16(INK4A), p16(INK4A), Mad2, TMS1/ASC, CASP8, and GSTP1. The clinical behavior of DLBCL after chemotherapy was followed up and analyzed...
  88. doi Hydrophobic bile acid-induced micronuclei formation, mitotic perturbations, and decreases in spindle checkpoint proteins: relevance to genomic instability in colon carcinogenesis
    Claire M Payne
    Department of Cell Biology and Anatomy, College of Medicine, University of Arizona, Tucson, AZ 85724 5044, USA
    Nutr Cancer 62:825-40. 2010
    ..basis for these mitosis-related perturbations included decreased expression of the spindle checkpoint proteins, Mad2, BubR1, and securin...
  89. doi Knockdown of Mad2 induces osteosarcoma cell apoptosis-involved Rad21 cleavage
    Ling Yu
    Department of Orthopedics, Renmin Hospital, Wuhan University, Wuhan, Hubei, China
    J Orthop Sci 16:814-20. 2011
    Besides Mad2's role in carcinogenesis, recent study has shown that it is essential in cell survival...
  90. pmc Closed MAD2 (C-MAD2) is selectively incorporated into the mitotic checkpoint complex (MCC)
    Aaron R Tipton
    Department of Biological Sciences, University of Toledo, Toledo, OH, USA
    Cell Cycle 10:3740-50. 2011
    ..b>MAD2 is an evolutionarily conserved mitotic checkpoint protein that exists in open (O) and closed (C) conformations...
  91. pmc Aurora kinase-A inactivates DNA damage-induced apoptosis and spindle assembly checkpoint response functions of p73
    Hiroshi Katayama
    Department of Molecular Pathology, The University of Texas M D Anderson Cancer Center, Houston, TX 77054, USA
    Cancer Cell 21:196-211. 2012
    ..Aurora-A phosphorylated p73 also facilitates inactivation of SAC through dissociation of the MAD2-CDC20 complex in cells undergoing mitosis...
  92. doi Involvement of multiple cell cycle aberrations in early preneoplastic liver cell lesions by tumor promotion with thioacetamide in a two-stage rat hepatocarcinogenesis model
    Masayuki Kimura
    Laboratory of Veterinary Pathology, Tokyo University of Agriculture and Technology, 3 5 8 Saiwai cho, Fuchu shi, Tokyo 183 8509, Japan
    Exp Toxicol Pathol 65:979-88. 2013
    ..of cell proliferation marker Ki-67, G2/M and spindle checkpoint proteins (phosphorylated checkpoint kinase 1 and Mad2), the DNA damage-related protein phosphorylated histone H2AX, and G2-M phase-related proteins (topoisomerase IIα, ..
  93. pmc A direct role of Mad1 in the spindle assembly checkpoint beyond Mad2 kinetochore recruitment
    Thomas Kruse
    The Novo Nordisk Foundation Center for Protein Research, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark
    EMBO Rep 15:282-90. 2014
    ..A key component of the SAC is the Mad2 protein, which can adopt either an inactive open (O-Mad2) or active closed (C-Mad2) conformation...
  94. pmc Molecular dynamics simulation on the conformational transition of the mad2 protein from the open to the closed state
    Chaoqun Li
    College of Chemistry, Beijing Normal University, 19 Xinjiekouwai Street, Beijing 100875, China
    Int J Mol Sci 15:5553-69. 2014
    The Mad2 protein, with two distinct conformations of open- and closed-states, is a key player in the spindle checkpoint. The closed Mad2 state is more active than the open one...
  95. pmc Mps1Mph1 Kinase Phosphorylates Mad3 to Inhibit Cdc20Slp1-APC/C and Maintain Spindle Checkpoint Arrests
    Judith Zich
    Wellcome Trust Centre for Cell Biology, Institute of Cell Biology, University of Edinburgh, Edinburgh, United Kingdom
    PLoS Genet 12:e1005834. 2016
    ..This genetic dissection of APC/C inhibition demonstrates that Mps1(Mph1) kinase-dependent modifications of Mad3 and Mad2 act in a concerted manner to maintain spindle checkpoint arrests.
  96. pmc Dimethyl Sulfoxide Perturbs Cell Cycle Progression and Spindle Organization in Porcine Meiotic Oocytes
    Xuan Li
    College of Animal Science and Technology, Northeast Agricultural University, Harbin, 150030, Heilongjiang, China
    PLoS ONE 11:e0158074. 2016
    ..3% DMSO treatment did not affect expression of genes involved in spindle organization (Bub1 and Mad2) and apoptosis (NF-ÎşB, Pten, Bcl2, Caspase3 and Caspase9), however, it significantly decreased expression levels ..
  97. doi Inhibition of CDK7 bypasses spindle assembly checkpoint via premature cyclin B degradation during oocyte meiosis
    Haiyang Wang
    Department of Animal Sciences, Chungbuk National University, Cheongju, Republic of Korea
    Biochim Biophys Acta 1863:2993-3000. 2016
    ..Notably, this acceleration occurred in the presence of SAC proteins including Mad2 and Bub3 at the kinetochores...
  98. pmc Generation of a Spindle Checkpoint Arrest from Synthetic Signaling Assemblies
    Ivan Yuan
    Wellcome Trust Centre for Cell Biology, University of Edinburgh King s Buildings, Max Born Crescent, Edinburgh EH9 3BF, UK
    Curr Biol . 2016
    ..This leads to dynamic kinetochore recruitment of Mad proteins [8, 9], a conformational change in Mad2 [10-12], and formation of the mitotic checkpoint complex (MCC: Cdc20-Mad3-Mad2 [13-15])...
  99. pmc The spindle checkpoint of budding yeast depends on a tight complex between the Mad1 and Mad2 proteins
    R H Chen
    Department of Physiology, University of California, San Francisco, San Francisco, California 94143 0444, USA
    Mol Biol Cell 10:2607-18. 1999
    ..We have cloned the MAD2 gene, which encodes a protein of 196 amino acids that remains at a constant level during the cell cycle...
  100. pmc The interaction between Ran and NTF2 is required for cell cycle progression
    B B Quimby
    Department of Biochemistry, Emory University School of Medicine, Atlanta, GA 30322, USA
    Mol Biol Cell 11:2617-29. 2000
    ..Moreover, when Ran is inefficiently imported into the nucleus, cells arrest in G(2) in a MAD2 checkpoint-dependent manner...
  101. pmc Inhibition of Cdh1-APC by the MAD2-related protein MAD2L2: a novel mechanism for regulating Cdh1
    C M Pfleger
    Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Genes Dev 15:1759-64. 2001
    ..Cdc20-APC is regulated through inhibition by the spindle assembly checkpoint protein MAD2. Knowledge of Cdh1-APC regulation is limited to the phosphorylation-dependent dissociation of Cdh1 from APC...

Research Grants52

  1. Kinetochore Function and Cell Cycle Progression Revision
    Katsumi Kitagawa; Fiscal Year: 2012
    ..Our synthetic-lethality screen using a mad2-deletion mutant against a set of yeast deletion mutations identified 32 genes, including those encoding previously ..
  2. CELL AND TUMOR GENETICS OF MITOTIC ARREST CHECKPOINTS
    PETER KARL SORGER; Fiscal Year: 2010
    ..will be analyzed in vitro and in vivo (2) The mechanism of action of a recently discovered negative regulator of Mad2 (CMT2) will be analyzed in detail to uncover key aspects of Mad2-mediated checkpoint signaling...
  3. Structure and Function of Spindle Checkpoint Proteins
    Xuelian Luo; Fiscal Year: 2012
    ..The spindle checkpoint protein Mad2 binds to Cdc20, the mitotic activator of APC/C, and inhibits APC/CCdc20, thus delaying the onset of anaphase...
  4. Role of Mitotic Checkpoint Defects in Mouse Tumor Models
    ROBERT I BENEZRA; Fiscal Year: 2010
    The mitotic checkpoint gene Mad2 has been shown in metazoans to be an important part of the mitotic checkpoint apparatus through biochemical and genetic experiments...
  5. KEVIN DANIEL CORBETT; Fiscal Year: 2016
    ..the roles of Hop1's conserved HORMA domain, a signaling domain shared with the spindle checkpoint protein Mad2, in regulating inter-homolog meiotic recombination, and in a meiosis-specific checkpoint monitoring recombination...
  6. James E Haber; Fiscal Year: 2016
    ..between the DNA damage checkpoint and the Spindle Assembly Checkpoint, following up our findings that deleting Mad2 shortens checkpoint arrest in wild type cells and suppresses the permanent arrest of adaptation-defective mutations ..
  7. Quantitative Measurements and Modeling of the Mitotic Checkpoint
    Jagesh V Shah; Fiscal Year: 2011
    ..Recent observations are now converging on a model whereby genes, particularly in the kinetochore-mediated Mad2 signaling pathway, are not necessarily mutated directly, but that the expression levels of genes and thereby ..
  8. MITOTIC CHECKPOINT GENE MAD2 IN VERTEBRATES
    Robert Benezra; Fiscal Year: 2004
    The mitotic checkpoint gene Mad2 has been shown in metazoans to be an important part of the mitotic checkpoint apparatus through biochemical experiments...
  9. Analysis of Loss of Function of MAD2 in Mammals
    Loren Michel; Fiscal Year: 2006
    ..have recently shown in both primary murine embryonic fibroblasts and human tumor cells that loss of one allele of MAD2, a mitotic checkpoint gene, is sufficient to cause chromosome instability...
  10. Metagenomic profiling of oral polymicrobial flora in head and neck cancers
    Charis Eng; Fiscal Year: 2011
    ..epigenetic alterations of target tumor suppressor genes in HNSCC;(3) To functionally validate the involvement of MAD2 and/or MDR1 in HNSCC tumor suppression...
  11. Wen Hwa Lee; Fiscal Year: 2014
    ..been increasingly recognized as a key regulator in mitotic processes including kinetochore-microtubule attachment, Mad2-dependent spindle assembly checkpoint activation and spindle assembly...
  12. Mechanism of Mitotic Checkpoint in Mammalian Cells
    TIMOTHY YEN; Fiscal Year: 2007
    ..hBUB 1 specifies the assembly of subdomain of the kinetochore that consists of checkpoint proteins hBUBR1, MAD1, MAD2 and Cdc20...
  13. John Pierce Wise; Fiscal Year: 2016
    ..cell division causing centrosome amplification focusing on Mps1 and Nek2A;3) Determine the role of Nek2A, Mad1 and Mad2 interactions in particulate Cr(VI)-induced spindle assembly checkpoint bypass;and 4) Identify those cellular and ..
  14. Mechanism of the Spindle Assembly Checkpoint Control
    Guowei Fang; Fiscal Year: 2006
    ..The activity of APC at metaphase is controlled by its activator CDC20 and by its inhibitor MAD2, a checkpoint protein...
  15. REGULATION OF THE ONSET OF ANAPHASE
    Tomohiro Matsumoto; Fiscal Year: 2001
    ..The goal of this proposal is to elucidate the function of two genes, s1p1+ and mad2+, both of which play key roles in regulating the onset of anaphase to insure accurate chromosome segregation...
  16. S WALTER WALTER ENGLANDER; Fiscal Year: 2016
    ..Study the role of transient partially unfolded states in other protein functions, with initial work directed at the Mad2 mitotic checkpoint protein...
  17. YsrRS regulon of Y. enterocolitica
    VIRGINIA MILLER; Fiscal Year: 2009
    ..enterocolitica strains. The Ysa TTSS is most closely related to the SPI-1 TTSS of Salmonella and Mxi-Spa TTSS of Shigella...
  18. IMMEDIATE-EARLY REGULATORY GENES OF CYTOMEGALOVIRUS
    Gary Hayward; Fiscal Year: 2007
    ..of post-transcriptional interactions of IE2 with four potential cell cycle regulatory proteins p21, JAB1, APC2 and MAD2; (II) continued exploration of the role of PML, DAXX, SUMO and the PODs at very early times in the nucleus, ..
  19. DIVERSE NATURAL PRODUCTS THAT STABILIZE MICROTUBULES
    Susan Horwitz; Fiscal Year: 2006
    ..HeLa cells will be transfected with MAD2, a checkpoint component, to determine if its overexpression can rescue from cell death, those cells that escape ..
  20. HMG-1/Y AND NEOPLASTIC TRANSFORMATION
    Linda Resar; Fiscal Year: 2002
    ..4) Explore the role of Mad, Mxi, and AP-2 in the regulation of HMG-I/Y...
  21. Functional Analysis of Mitotic Checkpoint Gene Bub1b
    Rakesh Goorha; Fiscal Year: 2005
    ..For example, haplo-insufficiency of mitotic checkpoint gene Mad2 causes chromosome missegregation leading to aneuploidy in mammalian cells and the development of lung tumors in ..
  22. FAT10 a candidate gene for epithelial apoptosis in HIV-associated nephropathy
    Michael Ross; Fiscal Year: 2007
    ..determine whether these SNPs alter the ability of FAT10 to associate with the mitotic spindle checkpoint protein MAD2. These studies will advance our understanding of HIVAN pathogenesis and may yield a novel therapeutic target for ..
  23. Mitotic Checkpoint & Genomic Stability in Ovarian Cancer
    Dong Yan Jin; Fiscal Year: 2006
    ..proteins MAD1 and CDH1; (b) study the transcriptional regulation of the expression of checkpoint proteins MAD1 and MAD2; (c) characterize the interaction of MAD1-MAD2 with the nuclear pore complex; and (d) identify the upstream signals ..
  24. The Mechanism of Lung Chemoprevention Mediated by ITCs
    Yang Ming Yang; Fiscal Year: 2005
    ..The presence of BubR1 at kinetochores greatly facilitates the binding of Bub3, Mad1, Mad2 and CENP-E, so BubR1 may integrate the signals for spindle check point regulation...
  25. MYC NETWORK FUNCTION IN THE DEVELOPMENT OF CANCER
    Leila Alland; Fiscal Year: 1999
    ..This interacting network of bHLH/LZ proteins consists of Myc,Max,Mad,and Mxi1. In addition to being an obligate partner in the transcriptionally competent Myc/Max complex,Max also appears to ..
  26. Analysis of Cdc20-Mad2 in spindle checkpoint signalling
    CLINTON COPELAND; Fiscal Year: 2006
    It has been well-documented that Mad1, Mad2, and Cdc20 have important roles in the spindle checkpoint in mitosis. In mammalian cells, Mad1 is responsible for Mad2 localization at the kinetochore...
  27. C-MYC REARRANGEMENTS IN HEMATOPOIETIC NEOPLASIAS
    Riccardo Dalla Favera; Fiscal Year: 2000
    ..Using this approach, we have identified two novel c-Myc target genes: I) Mxi which codes for a transcription factor which antagonizes the function of c-Myc by heterodimerizing with Max...
  28. Role of Alternative Mxi1 Isoforms in the Myc Network
    Daniel Wechsler; Fiscal Year: 2004
    ..Through these studies, we will gain a better understanding of the role of these Mxii isoforms in the complex Myc signaling pathways involved in cell growth regulation and neoplasia. ..
  29. REGULATION OF MYC NETWORK FUNCTION IN DEVELOPMENT
    Ronald DePinho; Fiscal Year: 1999
    ..of Myc are highly dependent upon its dimerization with Max and are opposed by two other bHLH/LZ proteins, Mad and Mxi1. The contrasting biochemical properties of Myc and Mad or Mxi1 have led to a working model for the regulation of ..
  30. 800 MHZ NMR SPECTROMETER
    JOSE RIZO REY; Fiscal Year: 2002
    ..structure and dynamics induced by natural and non-natural ligands; competition for the mitotic checkpoint protein Mad2 by its upstream regulator, Mad1, and its downstream target, Cdc20...
  31. Protection of Centromeric Cohesion by Bub1 and Sgo1
    Hongtao Yu; Fiscal Year: 2010
    ....
  32. Regulation of the Anaphase-Promoting Complex by the Spindle Checkpoint
    Hongtao Yu; Fiscal Year: 2010
    ..MCC contains BubR1, Bub3, Cdc20, and Mad2. BubR1 and Bub3 bind to each other constitutively throughout the cell cycle whereas the BubR1-Cdc20 and Mad2-Cdc20 ..
  33. Role of Nuf2 in Kinetochore Function
    Jennifer DeLuca; Fiscal Year: 2004
    ..abstract_text> ..
  34. SPINDLE ASSEMBLY CHECKPOINT IN VERTEBRATES AND YEAST
    Rey Huei Chen; Fiscal Year: 2003
    ..In addition to the biochemical studies, yeast genetics will be used to analyze Mad2 in detail and to identify other spindle assembly checkpoint components by suppressor screens...
  35. S6 Phosphorylation & Tyrosine Protein Kinases
    JAMES MALLER; Fiscal Year: 2004
    ..Experiments will evaluate whether Bub1 mediates CSF arrest by the MAPK pathway. This work provides a link between the MAPK/Rsk pathway and checkpoint control of the cell cycle at the metaphase/anaphase transition. ..
  36. NEGATIVE REGULATION OF CELL CYCLE PROGRESSION
    M Hoyt; Fiscal Year: 2004
    ..The identified genes will report checkpoint activation and will allow a powerful dissection of this signal transduction pathway. ..
  37. CONTROL OF CELL DIVISION IN XENOPUS OOCYTES
    JAMES MALLER; Fiscal Year: 2003
    ..Further work will examine the regulation, localization, and targets of PIx1 and identify kinases that activate xPIkk1. ..
  38. FUNCTION OF A DUAL SPECIFICITY TRANSCRIPTION FACTOR
    Peter Hurlin; Fiscal Year: 2003
    ..Finally, gene targeting in the mouse will be used to study the biological activities of Mga and the individual roles of its bHLHZip domain and T-domain. ..
  39. SHARED HIGH RESOLUTION MULTIMODE MICROSCOPE SYSTEM
    Greenfield Sluder; Fiscal Year: 2001
    ..The proposed instrument system will allow these researchers to take their investigations in new directions, obtain novel information, and thus, significantly advance their programs in basic biomedical research. ..
  40. ANALYSIS AND CONTROL OF CHROMOSOME MOVEMENT
    R Nicklas; Fiscal Year: 2005
    ..Kinetochores will be reconstructed by threedimensional tomography. The ultimate goal is to understand how tension, by altering structure, can lead to chemical changes such as dephosphorylation. ..
  41. Preventive of Bile Acid-Induced Colonic Oxidative DNA Damage
    Claire Margaret Payne; Fiscal Year: 2010
    ..129 mice) developed in our laboratory;2) NOS-/- k.o./DOC mouse model;3) arginine-enhanced colon tumor ApcMin/+ mouse model developed in our laboratory;4) the AOM mouse model of colon carcinogenesis using A/J mice. ..
  42. Myc Signaling in Medulloblastomas
    Charles Eberhart; Fiscal Year: 2006
    ..Finally, in Specific Aim 4 we propose developing a novel medulloblastoma transgenic model by overexpressing c-Myc in the cerebellum of transgenic mice. ..
  43. PREVENTION OF COLORECTAL CANCER BY iNOS AND COX-2 SELECTIVE INHIBITORS
    CHINTHALAPALLY RAO; Fiscal Year: 2008
    ..abstract_text> ..
  44. Myc Antagonism and Tumor Suppression by Mnt
    Peter Hurlin; Fiscal Year: 2008
    ..abstract_text> ..
  45. Cell Cycle Genes and Female Reproductive Function
    Hiroaki Kiyokawa; Fiscal Year: 2008
    ..No available cell line would generate such information. It is our expectation that this program contributes to a scientific foundation for therapeutic targets against infertility and other ovarian diseases. ..
  46. Regulation of the von Hippel-Lindau Protein
    Paul Corn; Fiscal Year: 2007
    ..abstract_text> ..
  47. Structure and function of the Mad2 checkpoint protein
    Xuelian Luo; Fiscal Year: 2007
    ..During her mentored training period, she will continue to study the structure and function of the Mad2 spindle checkpoint protein...
  48. MECHANISMS FOR CHEMOPREVENTION OF COLON CANCER
    CHINTHALAPALLY RAO; Fiscal Year: 2004
    ..Tissue distribution and comparative metabolism studies of curcumin and PEMC will be studied with synthetic [3H] curcumin and [3H]-PEMC in vivo in male F-344 rats. ..
  49. CHEMOKINES/CYTOKINES AND RECEPTORS IN STEM CELL HOMING
    Hal Broxmeyer; Fiscal Year: 2005
    ..These studies should clarity the relevance of SDF-1 and CXCR4 for homing/engraftment and mobilization of primary stem/progenitor cells. ..
  50. Cytochrome C Oxidase I:A Biomarker for Colon Cancer Risk
    Claire Payne; Fiscal Year: 2006
    ..Various molecular and cellular techniques will be used in these studies. ..
  51. Transcriptional Repression by the Myc Antagonist Mxil
    Nicole Schreiber Agus; Fiscal Year: 2006
    unreadable] DESCRIPTION (provided by applicant): The Mxi1 protein (and other members of the Mad/Mxi1 family) functions as a Myc antagonist and a growth/tumor suppressor, in part by effecting transcriptional repression of key target genes...
  52. Role of G1-Cdks in immortalization and oncogenesis
    Hiroaki Kiyokawa; Fiscal Year: 2006
    ..abstract_text> ..