MXI1

Summary

Gene Symbol: MXI1
Description: MAX interactor 1, dimerization protein
Alias: MAD2, MXD2, MXI, bHLHc11, max-interacting protein 1, MAX dimerization protein 2, Max-related transcription factor, class C basic helix-loop-helix protein 11
Species: human
Products:     MXI1

Top Publications

  1. Eagle L, Yin X, Brothman A, Williams B, Atkin N, Prochownik E. Mutation of the MXI1 gene in prostate cancer. Nat Genet. 1995;9:249-55 pubmed
    The Mxi1 protein negatively regulates Myc oncoprotein activity and thus potentially serves a tumour suppressor function. MXI1 maps to chromosome 10q24-q25, a region that is deleted in some cases of prostate cancer...
  2. Schreiber Agus N, Chin L, Chen K, Torres R, Rao G, Guida P, et al. An amino-terminal domain of Mxi1 mediates anti-Myc oncogenic activity and interacts with a homolog of the yeast transcriptional repressor SIN3. Cell. 1995;80:777-86 pubmed
    ..of Myc-responsive genes in which transactivation-competent Myc-Max heterodimers are opposed by repressive Mxi1-Max or Mad-Max complexes...
  3. Zervos A, Gyuris J, Brent R. Mxi1, a protein that specifically interacts with Max to bind Myc-Max recognition sites. Cell. 1993;72:223-32 pubmed
    We used the interaction trap to isolate a novel human protein that specifically interacts with Max. This protein, Mxi1 (for Max interactor 1), contains a bHLH-Zip motif that is similar to that found in Myc family proteins...
  4. Schreiber Agus N, Meng Y, Hoang T, Hou H, Chen K, Greenberg R, et al. Role of Mxi1 in ageing organ systems and the regulation of normal and neoplastic growth. Nature. 1998;393:483-7 pubmed
    b>Mxi1 belongs to the Mad (Mxi1) family of proteins, which function as potent antagonists of Myc oncoproteins...
  5. Manni I, Tunici P, Cirenei N, Albarosa R, Colombo B, Roz L, et al. Mxi1 inhibits the proliferation of U87 glioma cells through down-regulation of cyclin B1 gene expression. Br J Cancer. 2002;86:477-84 pubmed
    b>Mxi1 is a Mad family member that plays a role in cell proliferation and differentiation. To test the role of Mxi1 on tumorigenesis of glioma cells we transfected a CMV-driven MXI1 cDNA in U87 human glioblastoma cells...
  6. Wechsler D, Hawkins A, Li X, Jabs E, Griffin C, Dang C. Localization of the human Mxi1 transcription factor gene (MXI1) to chromosome 10q24-q25. Genomics. 1994;21:669-72 pubmed
  7. Edelhoff S, Ayer D, Zervos A, Steingrimsson E, Jenkins N, Copeland N, et al. Mapping of two genes encoding members of a distinct subfamily of MAX interacting proteins: MAD to human chromosome 2 and mouse chromosome 6, and MXI1 to human chromosome 10 and mouse chromosome 19. Oncogene. 1994;9:665-8 pubmed
    Both the MAD and the MXI1 genes encode basic-helix-loop-helix-leucine zipper (bHLH-Zip) transcription factors which bind Max in vitro, forming a sequence-specific DNA-binding complex similar to the Myc-Max heterodimer...
  8. Prochownik E, Eagle Grove L, Deubler D, Zhu X, Stephenson R, Rohr L, et al. Commonly occurring loss and mutation of the MXI1 gene in prostate cancer. Genes Chromosomes Cancer. 1998;22:295-304 pubmed
    ..We previously demonstrated that the MXI1 gene maps to 10q24-q25 and is mutated in some tumors with cytogenetically detectable deletions of this locus...
  9. Xu W, Liu M, Peng X, Zhou P, Zhou J, Xu K, et al. miR-24-3p and miR-27a-3p promote cell proliferation in glioma cells via cooperative regulation of MXI1. Int J Oncol. 2013;42:757-66 pubmed publisher
    ..By integrated bioinformatic analysis and experimental confirmation, we identified MXI1, which has been found to act as a tumor suppressor gene by affecting c?Myc, as a direct target of miR?..

More Information

Publications111 found, 100 shown here

  1. Huang Y, Huang X, Cai J, Ouyang Z, Wei S, Wei J, et al. Identification of orange-spotted grouper (Epinephelus coioides) interferon regulatory factor 3 involved in antiviral immune response against fish RNA virus. Fish Shellfish Immunol. 2015;42:345-52 pubmed publisher
    ..Moreover, the mRNA expression levels of type I IFN and IFN-inducible genes (MxI, ISG15 and ISG56) were increased in RGNNV infected EcIRF3 overexpressing cells compared to empty vector transfected ..
  2. Chapard C, Meraldi P, Gleich T, Bachmann D, Hohl D, Huber M. TRAIP is a regulator of the spindle assembly checkpoint. J Cell Sci. 2014;127:5149-56 pubmed publisher
    ..cells bypassed taxol-induced mitotic arrest and displayed significantly reduced kinetochore levels of MAD2 (also known as MAD2L1) but not of other spindle checkpoint proteins in the presence of nocodazole...
  3. Wang H, Jo Y, Sun T, Namgoong S, Cui X, Oh J, et al. Inhibition of CDK7 bypasses spindle assembly checkpoint via premature cyclin B degradation during oocyte meiosis. Biochim Biophys Acta. 2016;1863:2993-3000 pubmed publisher
    ..Notably, this acceleration occurred in the presence of SAC proteins including Mad2 and Bub3 at the kinetochores...
  4. Courtheoux T, Diallo A, Damodaran A, Reboutier D, Watrin E, Prigent C. Aurora A kinase activity is required to maintain an active spindle assembly checkpoint during prometaphase. J Cell Sci. 2018;131: pubmed publisher
    ..Aurora A activity directly affects SAC function, that Aurora A is essential for maintaining the checkpoint protein Mad2 on unattached kinetochores and that inhibition of Aurora A leads to loss of the SAC, even in the presence of ..
  5. Zhou W, Yin M, Cui H, Wang N, Zhao L, Yuan L, et al. Identification of potential therapeutic target genes and mechanisms in non-small-cell lung carcinoma in non-smoking women based on bioinformatics analysis. Eur Rev Med Pharmacol Sci. 2015;19:3375-84 pubmed
    ..DEGs including hyaluronan-mediated motility receptor (HMMR), collagen, type I alpha 2 (COL1A2), cyclin A2 (CCNA2), MAD2 mitotic arrest deficient-like 1 (MAD2L1), interleukin 6 (IL6) and interleukin 1, beta (IL1B) were identified in ..
  6. Spence J, Phua H, Mills W, Carpenter A, Porter A, Farr C. Depletion of topoisomerase IIalpha leads to shortening of the metaphase interkinetochore distance and abnormal persistence of PICH-coated anaphase threads. J Cell Sci. 2007;120:3952-64 pubmed
    ..the influence of sumoylated topo II on sister centromere cohesion; and its involvement in the activation of a Mad2-dependent spindle checkpoint...
  7. Edgerton H, Paolillo V, Oakley B. Spatial regulation of the spindle assembly checkpoint and anaphase-promoting complex in Aspergillus nidulans. Mol Microbiol. 2015;95:442-57 pubmed publisher
    ..have created functional fluorescent protein fusions of four SAC proteins in Aspergillus nidulans, the homologs of Mad2, Mps1, Bub1/BubR1 and Bub3...
  8. Sharma B, Kolhe R, Black S, Keller J, Mivechi N, Satyanarayana A. Inhibitor of differentiation 1 transcription factor promotes metabolic reprogramming in hepatocellular carcinoma cells. FASEB J. 2016;30:262-75 pubmed publisher
    ..and overexpression of oxygen-insensitive hypoxia-inducible factor 1α (Hif1α) or its downstream target Mxi1 resulted in a significant reduction of c-Myc and Id1 (∼70%), suggesting that Hif1α suppresses Id1 and c-Myc ..
  9. George A, Walworth N. Escape from Mitotic Arrest: An Unexpected Connection Between Microtubule Dynamics and Epigenetic Regulation of Centromeric Chromatin in Schizosaccharomyces pombe. Genetics. 2015;201:1467-78 pubmed publisher
    ..Suppression of the dis phenotype through loss of msc1 function requires the spindle checkpoint protein Mad2 and is limited by the presence of the heterochromatin-associated HP1 protein homolog Swi6...
  10. Silva R, Mirkovic M, Guilgur L, Rathore O, Martinho R, Oliveira R. Absence of the Spindle Assembly Checkpoint Restores Mitotic Fidelity upon Loss of Sister Chromatid Cohesion. Curr Biol. 2018;28:2837-2844.e3 pubmed publisher
    ..Surprisingly, knockdown of key SAC proteins, Mad2 and Mps1, suppressed developmental defects associated with San depletion...
  11. Lee K, Kenny A, Rieder C. P38 mitogen-activated protein kinase activity is required during mitosis for timely satisfaction of the mitotic checkpoint but not for the fidelity of chromosome segregation. Mol Biol Cell. 2010;21:2150-60 pubmed publisher
    ..However, under this condition chromatid segregation and cytokinesis are normal. Using Mad2/YFP-expressing cells, we show the prolongation of mitosis in the absence of p38 activity is directly due to a delay ..
  12. Wood L, Booth D, Vargiu G, Ohta S, deLima Alves F, Samejima K, et al. Auxin/AID versus conventional knockouts: distinguishing the roles of CENP-T/W in mitotic kinetochore assembly and stability. Open Biol. 2016;6:150230 pubmed publisher
    ..Interestingly, a putative super-complex involving Rod-Zw10-zwilch (RZZ complex), Spindly, Mad1/Mad2 and CENP-E requires the function of CENP-T/W during kinetochore assembly for its stable association with the outer ..
  13. Bentley A, Normand G, Hoyt J, King R. Distinct sequence elements of cyclin B1 promote localization to chromatin, centrosomes, and kinetochores during mitosis. Mol Biol Cell. 2007;18:4847-58 pubmed
    ..Kinetochore recruitment of cyclin B1 required the kinetochore proteins Hec1 and Mad2, and it was stimulated by microtubule destabilization...
  14. Ibrahim B. Systems Biology Modeling of Five Pathways for Regulation and Potent Inhibition of the Anaphase-Promoting Complex (APC/C): Pivotal Roles for MCC and BubR1. OMICS. 2015;19:294-305 pubmed publisher
    ..In this study, five possible model variants of APC/C regulation were constructed, namely BubR1, Mad2, MCC, MCF2, and an all-pathways model variant...
  15. Yu Y, Huang X, Liu J, Zhang J, Hu Y, Yang Y, et al. Fish TRIM32 functions as a critical antiviral molecule against iridovirus and nodavirus. Fish Shellfish Immunol. 2017;60:33-43 pubmed publisher
    ..regulatory factor 3 (IRF3), IRF7, interferon-stimulated gene 15 (ISG15), interferon-induced 35-kDa protein (IFP35), MXI, TIR-domain-containing adaptor-inducing interferon-? (TRIF) and melanoma differentiation-associated protein 5 (MDA5)..
  16. Yang Y, Huang Y, Yu Y, Zhou S, Wang S, Yang M, et al. Negative regulation of the innate antiviral immune response by TRIM62 from orange spotted grouper. Fish Shellfish Immunol. 2016;57:68-78 pubmed publisher
    ..gene 15 (ISG15), melanoma differentiation-associated protein 5 (MDA5), myxovirus resistance gene MXI, and MXII, suggesting that the negative regulation of interferon immune response by EcTRIM62 might directly ..
  17. Marcozzi C, Pines J. Assays for the spindle assembly checkpoint in cell culture. Methods Cell Biol. 2018;144:1-13 pubmed publisher
    ..kinetochores activate the SAC and catalyze the formation of the mitotic checkpoint complex (MCC), containing Mad2, Cdc20, BubR1, and Bub3 proteins...
  18. Liang Y, Zhong Z, Huang Y, Deng W, Cao J, Tsao G, et al. Stem-like cancer cells are inducible by increasing genomic instability in cancer cells. J Biol Chem. 2010;285:4931-40 pubmed publisher
    ..Likewise, either overexpression of a key regulator of cell cycle, Mad2, or knock down of Aurora B, an important kinase in mitosis, or Cdh1, a key E3 ligase in cell cycle, resulted in a ..
  19. Zich J, May K, Paraskevopoulos K, Sen O, Syred H, van der Sar S, et al. Mps1Mph1 Kinase Phosphorylates Mad3 to Inhibit Cdc20Slp1-APC/C and Maintain Spindle Checkpoint Arrests. PLoS Genet. 2016;12:e1005834 pubmed publisher
    ..This genetic dissection of APC/C inhibition demonstrates that Mps1(Mph1) kinase-dependent modifications of Mad3 and Mad2 act in a concerted manner to maintain spindle checkpoint arrests.
  20. Jo J, Lee J, Jee B, Suh C, Kim S. Exposing mouse oocytes to necrostatin 1 during in vitro maturation improves maturation, survival after vitrification, mitochondrial preservation, and developmental competence. Reprod Sci. 2015;22:615-25 pubmed publisher
    ..The 1 μmol/L Nec1 showed significantly increased expressions of Mad2, Gdf9, and Bcl2. The Cirp level had a tendency to be downregulated in the 0...
  21. Yu Y, Huang Y, Yang Y, Wang S, Yang M, Huang X, et al. Negative regulation of the antiviral response by grouper LGP2 against fish viruses. Fish Shellfish Immunol. 2016;56:358-366 pubmed publisher
    ..decreased the expression level of interferon related molecules or effectors, including IRF3, IRF7, ISG15, IFP35, MXI, MXII, and MDA5, suggesting that the negative feedback of interferon immune response by EcLGP2 might contribute to ..
  22. Geerts D, Koster J, Albert D, Koomoa D, Feith D, Pegg A, et al. The polyamine metabolism genes ornithine decarboxylase and antizyme 2 predict aggressive behavior in neuroblastomas with and without MYCN amplification. Int J Cancer. 2010;126:2012-24 pubmed publisher
    ..003). In addition, we provide first evidence of a role for MYCN-associated transcription factors MAD2 and MAD7 in ODC regulation...
  23. Weyler L, Engelbrecht M, Mata Forsberg M, Brehwens K, Vare D, Vielfort K, et al. Restriction endonucleases from invasive Neisseria gonorrhoeae cause double-strand breaks and distort mitosis in epithelial cells during infection. PLoS ONE. 2014;9:e114208 pubmed publisher
    ..Infected cells show dysregulation of the spindle assembly checkpoint proteins MAD1 and MAD2, impaired and prolonged M-phase, nuclear swelling, micronuclei formation and chromosomal instability...
  24. Woo Seo D, Yeop You S, Chung W, Cho D, Kim J, Su Oh J. Zwint-1 is required for spindle assembly checkpoint function and kinetochore-microtubule attachment during oocyte meiosis. Sci Rep. 2015;5:15431 pubmed publisher
    ..Knockdown of Zwint-1 accelerated the first meiosis by abrogating the kinetochore recruitment of Mad2, leading to chromosome misalignment and a high incidence of aneuploidy...
  25. Li J, Dang N, Wood D, Huang J. The kinetochore-dependent and -independent formation of the CDC20-MAD2 complex and its functions in HeLa cells. Sci Rep. 2017;7:41072 pubmed publisher
    The mitotic checkpoint complex (MCC) is formed from two sub-complexes of CDC20-MAD2 and BUBR1-BUB3, and current models suggest that it is generated exclusively by the kinetochores after nuclear envelope breakdown (NEBD)...
  26. Zhang G, Lischetti T, Hayward D, Nilsson J. Distinct domains in Bub1 localize RZZ and BubR1 to kinetochores to regulate the checkpoint. Nat Commun. 2015;6:7162 pubmed publisher
    ..Checkpoint signalling requires the kinetochore localization of the Mad1-Mad2 complex that in more complex eukaryotes depends on the Rod-Zwilch-ZW10 (RZZ) complex...
  27. Shin H, Park E, Yun S, Kim S, Jung W, Woo S, et al. p31comet-Induced Cell Death Is Mediated by Binding and Inactivation of Mad2. PLoS ONE. 2015;10:e0141523 pubmed publisher
    b>Mad2, a key component of the spindle checkpoint, is closely associated with chromosomal instability and poor prognosis in cancer. p31comet is a Mad2-interacting protein that serves as a spindle checkpoint silencer at mitosis...
  28. Prasad S, Koch B, Chaube S. Involvement of Cyclin-Dependent Kinase 1 during Postovulatory Aging-Mediated Abortive Spontaneous Egg Activation in Rat Eggs Cultured In Vitro. Cell Reprogram. 2016;18:96-107 pubmed publisher
    ..was significantly reduced, but anaphase promoting complex/cyclosome (APC/C) and mitotic arrest deficient protein (MAD2) levels were increased initially and then reduced during a later period of in vitro culture...
  29. Vander Broek C, Stevens J. Type III Secretion in the Melioidosis Pathogen Burkholderia pseudomallei. Front Cell Infect Microbiol. 2017;7:255 pubmed publisher
    ..T3SS-3 is better characterized and is homologous to the Inv/Mxi-Spa secretion systems of Salmonella spp. and Shigella flexneri, respectively...
  30. Yang S, Hung C, Aljuffali I, Fang J. The roles of the virulence factor IpaB in Shigella spp. in the escape from immune cells and invasion of epithelial cells. Microbiol Res. 2015;181:43-51 pubmed publisher
    ..Many virulence factors are encoded in the ipa-mxi-spa region by the virulence plasmid...
  31. Musacchio A. Closing the Mad2 cycle. elife. 2015;4: pubmed publisher
    ..Chromosome separation is regulated by a cycle that involves a protein undergoing an unusual topological conversion. ..
  32. Chmielewska A, Tang N, Toda T. The hairpin region of Ndc80 is important for the kinetochore recruitment of Mph1/MPS1 in fission yeast. Cell Cycle. 2016;15:740-7 pubmed publisher
    ..checkpoint (SAC), which blocks anaphase onset via recruitment of a cohort of SAC components (Mph1/MPS1, Mad1, Mad2, Mad3/BubR1, Bub1 and Bub3) to kinetochores...
  33. Prasad S, Koch B, Chaube S. Maturation promoting factor destabilization facilitates postovulatory aging-mediated abortive spontaneous egg activation in rat. Dev Growth Differ. 2016;58:293-302 pubmed publisher
    ..inhibitor 2 (Emi2), anaphase promoting complex/cyclosome (APC/C), cyclin B1, mitotic arrest deficient protein (MAD2) levels and Cdk1 activity were analyzed...
  34. Singh G, Karade S, Ranjan R, Ahamad N, Ahmed S. C-terminal region of Mad2 plays an important role during mitotic spindle checkpoint in fission yeast Schizosaccharomyces pombe. Mol Biol Rep. 2017;44:89-96 pubmed publisher
    The mitotic arrest deficiency 2 (Mad2) protein is an essential component of the spindle assembly checkpoint that interacts with Cdc20/Slp1 and inhibit its ability to activate anaphase promoting complex/cyclosome (APC/C)...
  35. Silió V, McAinsh A, Millar J. KNL1-Bubs and RZZ Provide Two Separable Pathways for Checkpoint Activation at Human Kinetochores. Dev Cell. 2015;35:600-613 pubmed publisher
    ..Activation of the SAC occurs through a series of ordered molecular events that result in recruitment of Mad1:Mad2 complexes to improperly attached kinetochores...
  36. Zhou H, Wang T, Zheng T, Teng J, Chen J. Cep57 is a Mis12-interacting kinetochore protein involved in kinetochore targeting of Mad1-Mad2. Nat Commun. 2016;7:10151 pubmed publisher
    ..Kinetochore accumulation of the SAC component Mad1-Mad2 is crucial for SAC activation...
  37. Weng S, Matsuura S, Mowery C, Stoner S, Lam K, Ran D, et al. Restoration of MYC-repressed targets mediates the negative effects of GM-CSF on RUNX1-ETO leukemogenicity. Leukemia. 2017;31:159-169 pubmed publisher
    ..agreement with this, a functional screen of a subset of GM-CSF-responsive genes demonstrated that a MYC inhibitor, MXI1 (Max interactor 1), reduced the leukemic potential of RE HSPCs and t(8;21) acute myeloid leukemia (AML) cells...
  38. Faesen A, Thanasoula M, Maffini S, Breit C, Müller F, van Gerwen S, et al. Basis of catalytic assembly of the mitotic checkpoint complex. Nature. 2017;542:498-502 pubmed publisher
    ..The HORMA-domain-containing protein mitotic arrest deficient 2 (MAD2) is a subunit of the SAC effector mitotic checkpoint complex (MCC)...
  39. Liu Y, Singh S, Zeng X, Zhao J, Hou S. The Nuclear Matrix Protein Megator Regulates Stem Cell Asymmetric Division through the Mitotic Checkpoint Complex in Drosophila Testes. PLoS Genet. 2015;11:e1005750 pubmed publisher
    ..Expression of mitotic arrest-deficient-2 (Mad2) and monopolar spindle 1 (Mps1) of the SAC complex effectively rescued the GSC loss phenotype associated with loss ..
  40. Zhang Q, Teng J, Zou G, Peng Q, Du Q, Jiao T, et al. Efficient phosphate sequestration for water purification by unique sandwich-like MXene/magnetic iron oxide nanocomposites. Nanoscale. 2016;8:7085-93 pubmed publisher
    ..Herein, a novel sandwiched structural 2D MXene-iron oxide (MXI) material, prepared by selectively exfoliating an Al layer followed by magnetic ferric oxide intercalation, ..
  41. Yuan Y, Ren Y, Yuan P, Yan L, Qiao J. TRAIP is involved in chromosome alignment and SAC regulation in mouse oocyte meiosis. Sci Rep. 2016;6:29735 pubmed publisher
    ..TRAIP knockdown decreased the accumulation of Mad2 on centromeres, potentially explaining why oocyte maturation was not affected following formation of DNA lesions...
  42. Wu D, Wang L, Yang Y, Huang J, Hu Y, Shu Y, et al. MAD2-p31comet axis deficiency reduces cell proliferation, migration and sensitivity of microtubule-interfering agents in glioma. Biochem Biophys Res Commun. 2018;498:157-163 pubmed publisher
    Mitotic arrest deficient-like-1 (MAD2, also known as MAD2L1) is thought to be an important spindle assembly checkpoint protein, which ensures accurate chromosome segregation and is closely associated with poor prognosis in many cancer...
  43. Olbryt M, Habryka A, Tyszkiewicz T, Rusin A, Cichon T, Jarząb M, et al. Melanoma-associated genes, MXI1, FN1, and NME1, are hypoxia responsive in murine and human melanoma cells. Melanoma Res. 2011;21:417-25 pubmed publisher
    ..and activator of transcription 3 (Stat3), microphthalmia-associated transcription factor (Mitf), max interacting protein 1 (Max1), and non-metastatic cells 1, protein (NM23A) expressed in (Nme1) are known to be associated with ..
  44. Nelson C, Hwang T, Chen P, Bhalla N. TRIP13PCH-2 promotes Mad2 localization to unattached kinetochores in the spindle checkpoint response. J Cell Biol. 2015;211:503-16 pubmed publisher
    ..During checkpoint activation, Mad1 recruits Mad2 to kinetochores to generate a signal that delays anaphase onset...
  45. Sisinni L, Maddalena F, Condelli V, Pannone G, Simeon V, Li Bergolis V, et al. TRAP1 controls cell cycle G2-M transition through the regulation of CDK1 and MAD2 expression/ubiquitination. J Pathol. 2017;243:123-134 pubmed publisher
    ..of key cell cycle regulators through a dual mechanism: (i) transcriptional regulation of CDK1, CYCLIN B1, and MAD2, as suggested by gene expression profiling of TRAP1-silenced breast carcinoma cells; and (ii) post-transcriptional ..
  46. Long Q, Xu J, Osunkoya A, Sannigrahi S, Johnson B, Zhou W, et al. Global transcriptome analysis of formalin-fixed prostate cancer specimens identifies biomarkers of disease recurrence. Cancer Res. 2014;74:3228-37 pubmed publisher
    ..have been associated with cancer, with five specifically associated with prostate cancer (BTG2, IGFBP3, SIRT1, MXI1, and FDPS)...
  47. Vleugel M, Hoek T, Tromer E, Sliedrecht T, Groenewold V, Omerzu M, et al. Dissecting the roles of human BUB1 in the spindle assembly checkpoint. J Cell Sci. 2015;128:2975-82 pubmed publisher
    ..catalyze the formation of a diffusible APC/C(CDC20) inhibitor that comprises BUBR1 (also known as BUB1B), BUB3, MAD2 (also known as MAD2L1) and a second molecule of CDC20...
  48. Sitry Shevah D, Kaisari S, Teichner A, Miniowitz Shemtov S, Hershko A. Role of ubiquitylation of components of mitotic checkpoint complex in their dissociation from anaphase-promoting complex/cyclosome. Proc Natl Acad Sci U S A. 2018;115:1777-1782 pubmed publisher
    ..It promotes the assembly of a mitotic checkpoint complex (MCC), composed of BubR1, Bub3, Cdc20, and Mad2, which inhibits the activity of the anaphase-promoting complex/cyclosome (APC/C) ubiquitin ligase...
  49. Zhang T, Zhou Y, Wang H, Meng T, Guo L, Ma X, et al. Spc24 is required for meiotic kinetochore-microtubule attachment and production of euploid eggs. Oncotarget. 2016;7:71987-71997 pubmed publisher
    ..and accelerated the first meiosis by abrogating the kinetochore recruitment of spindle assembly checkpoint protein Mad2, leading to a high incidence of aneuploidy...
  50. Hu Z, Dong N, Lu D, Jiang X, Xu J, Wu Z, et al. A positive feedback loop between ROS and Mxi1-0 promotes hypoxia-induced VEGF expression in human hepatocellular carcinoma cells. Cell Signal. 2017;31:79-86 pubmed publisher
    ..ROS mediates hypoxia-induced VEGF by upregulation of Mxi1-0...
  51. Albarosa R, DiDonato S, Finocchiaro G. Redefinition of the coding sequence of the MXI1 gene and identification of a polymorphic repeat in the 3' non-coding region that allows the detection of loss of heterozygosity of chromosome 10q25 in glioblastomas. Hum Genet. 1995;95:709-11 pubmed
    The MXI1 gene encodes a protein interacting with Max, a regulatory factor of the Myc oncogene, and is located on chromosome 10q25, a region showing frequent loss of heterozygosity in malignant gliomas...
  52. Billin A, Eilers A, Queva C, Ayer D. Mlx, a novel Max-like BHLHZip protein that interacts with the Max network of transcription factors. J Biol Chem. 1999;274:36344-50 pubmed
    ..In contrast with Max, Mlx interacts only with Mad1 and Mad4. Together, these findings suggest that Mlx may act to diversify Mad family function by its restricted association with a subset of the Mad family of transcriptional repressors. ..
  53. Engstrom L, Youkilis A, Gorelick J, Zheng D, Ackley V, Petroff C, et al. Mxi1-0, an alternatively transcribed Mxi1 isoform, is overexpressed in glioblastomas. Neoplasia. 2004;6:660-73 pubmed
    The c-Myc transcription factor regulates expression of genes related to cell growth, division, and apoptosis. Mxi1, a member of the Mad family, represses transcription of c-Myc-regulated genes by mediating chromatin condensation via ..
  54. Zhang K, Faiola F, Martinez E. Six lysine residues on c-Myc are direct substrates for acetylation by p300. Biochem Biophys Res Commun. 2005;336:274-80 pubmed
    ..Our results further indicate that p300 can acetylate DNA-bound Myc:Max complexes and that acetylated Myc:Max heterodimers efficiently interact with Miz-1. ..
  55. Delpuech O, Griffiths B, East P, Essafi A, Lam E, Burgering B, et al. Induction of Mxi1-SR alpha by FOXO3a contributes to repression of Myc-dependent gene expression. Mol Cell Biol. 2007;27:4917-30 pubmed
    ..activation induced expression of several members of the Mad/Mxd family of transcriptional repressors, most notably Mxi1. The induction of Mxi1 by FOXO3a was specific to the Mxi1-SR alpha isoform and was mediated by three highly ..
  56. Tsao C, Teh B, Jonasch E, Shreiber Agus N, Efstathiou E, Hoang A, et al. Inhibition of Mxi1 suppresses HIF-2alpha-dependent renal cancer tumorigenesis. Cancer Biol Ther. 2008;7:1619-27 pubmed
    ..b>Mxi1, a c-Myc antagonist, is a HIF target gene that inhibits mitochondrial biogenesis, reprograms cellular energy ..
  57. Chan A, Singh A, Northcote P, Miller J. Peloruside A, a microtubule-stabilizing agent, induces aneuploidy in ovarian cancer cells. Invest New Drugs. 2016;34:424-38 pubmed publisher
    ..effects of peloruside A, a microtubule-stabilizing agent, on expression levels of several key cell cycle proteins, MAD2, BUBR1, p55CDC and cyclin B1...
  58. Aichem A, Groettrup M. The ubiquitin-like modifier FAT10 in cancer development. Int J Biochem Cell Biol. 2016;79:451-461 pubmed publisher
    ..Moreover, FAT10 directly interacts with and influences downstream targets such as MAD2, p53 or ?-catenin, leading to enhanced survival, proliferation, invasion and metastasis formation of cancer cells ..
  59. Quartuccio S, Dipali S, Schindler K. Haspin inhibition reveals functional differences of interchromatid axis-localized AURKB and AURKC. Mol Biol Cell. 2017;28:2233-2240 pubmed publisher
    ..is the sole CPC kinase, does not alter MI completion timing, and no change in localization of the SAC protein, MAD2, is observed...
  60. Zervos A, Gyuris J, Brent R. Mxi1, a protein that specifically interacts with Max to bind Myc-Max recognition sites. Cell. 1994;79:following 388 pubmed
  61. Chen R, Waters J, Salmon E, Murray A. Association of spindle assembly checkpoint component XMAD2 with unattached kinetochores. Science. 1996;274:242-6 pubmed
    ..A frog homolog of MAD2 (XMAD2) was isolated and found to play an essential role in the spindle assembly checkpoint in frog egg extracts...
  62. Dugast Darzacq C, Grange T, Schreiber Agus N. Differential effects of Mxi1-SRalpha and Mxi1-SRbeta in Myc antagonism. FEBS J. 2007;274:4643-53 pubmed
    b>Mxi1 belongs to the Myc-Max-Mad transcription factor network. Two Mxi1 protein isoforms, Mxi1-SRalpha and Mxi1-SRbeta, have been described as sharing many biological properties...
  63. Nascimento A, Singh A, Bousbaa H, Ferreira D, Sarmento B, Amiji M. Overcoming cisplatin resistance in non-small cell lung cancer with Mad2 silencing siRNA delivered systemically using EGFR-targeted chitosan nanoparticles. Acta Biomater. 2017;47:71-80 pubmed publisher
    ..chitosan (CS) nanoparticles as a versatile delivery system for silencing the essential mitotic checkpoint gene Mad2, and induce cell death...
  64. Tzeng Y, Li D, Chen Y, Yang C, Chang C, Juang Y. LMO7 exerts an effect on mitosis progression and the spindle assembly checkpoint. Int J Biochem Cell Biol. 2018;94:22-30 pubmed publisher
    ..LIM domain of LMO7 was a determinant of its ability to interfere with kinetochore localization of the SAC proteins MAD2 and BUBR1 and cause a SAC defect though the LIM peptide itself did neither bind to MAD1, MAD2 and BUBR1 nor ..
  65. Shimizu E, Shirasawa H, Kodama K, Sato T, Simizu B. Expression, regulation and polymorphism of the mxi1 genes. Gene. 1996;176:45-8 pubmed
    The myc proto-oncogene family plays an important role in the control of cellular growth and differentiation. Mxi1, one of the Max-associated proteins, has been known to have an antagonistic action on Myc activity...
  66. FitzGerald M, Arsura M, Bellas R, Yang W, Wu M, Chin L, et al. Differential effects of the widely expressed dMax splice variant of Max on E-box vs initiator element-mediated regulation by c-Myc. Oncogene. 1999;18:2489-98 pubmed
    ..In in vitro protein:protein association assays, dMax interacted with c-Myc, N-Myc, L-Myc, Mad1, Mxi1, Mad3 and Mad4, but not with itself or wild-type Max. These interactions required an intact leucine zipper...
  67. Ariyanayagam Baksh S, Baksh F, Swalsky P, Finkelstein S. Loss of heterozygosity in the MXI1 gene is a frequent occurrence in melanoma. Mod Pathol. 2003;16:992-5 pubmed
    ..3. The MXI1 gene at 10q24-25 is another candidate tumor suppressor that has only rarely been studied in melanomas, with ..
  68. Scott D, Straughton D, Cole M, Bailey S, Ellison D, Clifford S. Identification and analysis of tumor suppressor loci at chromosome 10q23.3-10q25.3 in medulloblastoma. Cell Cycle. 2006;5:2381-9 pubmed
    ..7Mb common interval, affecting the region 10q23.3-10q25.3. This region contains three genes, MXI1, SUFU and BTRC, which represent putative medulloblastoma tumor suppressor (TS) genes on the basis of either (i) ..
  69. Ólafsson G, Thorpe P. Synthetic Physical Interactions Map Kinetochore-Checkpoint Activation Regions. G3 (Bethesda). 2016;6:2531-42 pubmed publisher
    ..The recruitment of the Mad1 and Mad2 proteins to the kinetochore is normally necessary for SAC activation...
  70. Byrnes A, Slep K. TOG-tubulin binding specificity promotes microtubule dynamics and mitotic spindle formation. J Cell Biol. 2017;216:1641-1657 pubmed publisher
    ..b>Mad2 knockdown released the spindle assembly checkpoint triggered when TOG5-microtubule binding was compromised, ..
  71. Borges D, Dos Santos A, Paier C, Ribeiro H, Costa M, Farias I, et al. Prognostic importance of Aurora Kinases and mitotic spindle genes transcript levels in Myelodysplastic syndrome. Leuk Res. 2018;64:61-70 pubmed publisher
    ..Proteins related to the mitotic spindle (AURKA, AURKB, TPX2), to the mitotic checkpoint (MAD2, CDC20) and the regulation of the cell cycle (p21) are directly related to chromosomal stability and tumor ..
  72. Benson L, Coon M, Krueger L, Han G, Sarnaik A, Wechsler D. Expression of MXI1, a Myc antagonist, is regulated by Sp1 and AP2. J Biol Chem. 1999;274:28794-802 pubmed
    b>MXI1, a member of the MAD family of Myc antagonists, encodes a transcription factor whose expression must be tightly regulated to maintain normal cell growth and differentiation...
  73. Taj M, Tawil R, Engstrom L, Zeng Z, Hwang C, Sanda M, et al. Mxi1, a Myc antagonist, suppresses proliferation of DU145 human prostate cells. Prostate. 2001;47:194-204 pubmed
    b>Mxi1, an antagonist of c-Myc, maps to human chromosome 10q24-q25, a region altered in a substantial fraction of prostate tumors. Mice deficient for Mxi1 exhibit significant prostate hyperplasia...
  74. Kawamata N, Sugimoto K, Sakajiri S, Oshimi K, Koeffler H. Mxi1 isoforms are expressed in hematological cell lines and normal bone marrow. Int J Oncol. 2005;26:1369-75 pubmed
    b>Mxi1 protein is a basic helix-loop-helix, leucine zipper (bHLHZIP) transcriptional factor, which dimerizes with the Max protein. This heterodimer binds a specific DNA sequence (E-box) and suppresses transcription of target genes...
  75. Löfstedt T, Fredlund E, Noguera R, Navarro S, Holmquist Mengelbier L, Beckman S, et al. HIF-1alpha induces MXI1 by alternate promoter usage in human neuroblastoma cells. Exp Cell Res. 2009;315:1924-36 pubmed publisher
    ..In this report we show that the MAX interactor-1 (MXI1) gene is directly regulated by HIF proteins in neuroblastoma and breast cancer cells...
  76. Armstrong M, Mody R, Ellis D, Hill A, Erichsen D, Wechsler D. N-Myc differentially regulates expression of MXI1 isoforms in neuroblastoma. Neoplasia. 2013;15:1363-70 pubmed
    ..Overexpression of N-Myc leads to transcriptional activation and an increase in NB cell proliferation. Mxi1, a member of the Myc family of transcriptional regulators, also binds to Max...
  77. Shirnekhi H, Kelley E, DeLuca J, Herman J. Spindle assembly checkpoint signaling and sister chromatid cohesion are disrupted by HPV E6-mediated transformation. Mol Biol Cell. 2017;28:2035-2041 pubmed publisher
    ..This SAC defect is caused in part by a loss of kinetochore-associated Mad2 in E6-expressing cells...
  78. Wu W, Hu Z, Wang F, Gu H, Jiang X, Xu J, et al. Mxi1-0 regulates the growth of human umbilical vein endothelial cells through extracellular signal-regulated kinase 1/2 (ERK1/2) and interleukin-8 (IL-8)-dependent pathways. PLoS ONE. 2017;12:e0178831 pubmed publisher
    b>Mxi1 plays an important role in the regulation of cell proliferation. Mxi1-0, a Mxi1 isoform, has a different N-terminal amino acid sequence, intracellular location and expression profile from Mxi1...
  79. Ji W, Luo Y, Ahmad E, Liu S. Direct interactions of mitotic arrest deficient 1 (MAD1) domains with each other and MAD2 conformers are required for mitotic checkpoint signaling. J Biol Chem. 2018;293:484-496 pubmed publisher
    ..amplification reaction for the checkpoint is the conformational conversion of "open" mitotic arrest deficient 2 (O-MAD2) into "closed" MAD2 (C-MAD2)...
  80. Chen J, Adamiak W, Huang G, Atasoy U, Rostami A, Yu S. Interaction of RNA-binding protein HuR and miR-466i regulates GM-CSF expression. Sci Rep. 2017;7:17233 pubmed publisher
    ..Furthermore, we found that HuR promoted Mxi1 expression to inhibit certain miRNA expression...
  81. Estojak J, Brent R, Golemis E. Correlation of two-hybrid affinity data with in vitro measurements. Mol Cell Biol. 1995;15:5820-9 pubmed
    ..For the calibration, we used two sets of proteins, the Myc-Max-Mxi1 helix-loop-helix proteins, and wild-type and dimerization-defective versions of the lambda cI repressor...
  82. Wechsler D, Shelly C, Dang C. Genomic organization of human MXI1, a putative tumor suppressor gene. Genomics. 1996;32:466-70 pubmed
    b>MXI1, a member of the MYC family of transcription factors, is thought to negatively regulate MYC function and may therefore be a potential tumor suppressor gene...
  83. Gupta K, Anand G, Yin X, Grove L, Prochownik E. Mmip1: a novel leucine zipper protein that reverses the suppressive effects of Mad family members on c-myc. Oncogene. 1998;16:1149-59 pubmed
    ..Four additional bHLH-ZIP proteins, Mad1, Mxi1, Mad3 and Mad4, heterodimerize with Max and also repress transcription of c-myc-responsive genes...
  84. Grinberg A, Hu C, Kerppola T. Visualization of Myc/Max/Mad family dimers and the competition for dimerization in living cells. Mol Cell Biol. 2004;24:4294-308 pubmed
    ..Complexes formed by Max with Mxi1, Mad3, or Mad4 were enriched in nuclear foci, whereas complexes formed with Myc were more uniformly distributed in ..
  85. Dugast Darzacq C, Pirity M, Blanck J, Scherl A, Schreiber Agus N. Mxi1-SRalpha: a novel Mxi1 isoform with enhanced transcriptional repression potential. Oncogene. 2004;23:8887-99 pubmed
    b>Mxi1 belongs to the Myc/Max/Mad network of proteins that have been implicated in the control of multiple aspects of cellular behavior...
  86. Wang Z, Lai F. [Analysis of loss of heterozygosity on chromosome 10 in human prostate carcinoma and high grade prostatic intraepithelial neoplasia]. Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2004;21:515-7 pubmed
    ..The rate of LOH in high grade PIN was much lower than that in prostate carcinoma. PTEN and MXI1 were two candidate tumor suppressor genes on 10q23 and 10q24-q25...
  87. Guo X, Pan L, Zhang X, Suo X, Niu Z, Zhang J, et al. Expression and mutation analysis of genes that encode the Myc antagonists Mad1, Mxi1 and Rox in acute leukaemia. Leuk Lymphoma. 2007;48:1200-7 pubmed
    The Myc antagonists Mad1, Mxi1 and Rox proteins share two highly conserved domains, Sin3-interacting domain (SID) and basic helix-loop-helix leucine zipper domain (bHLHzip), which are essential for these proteins to function during ..
  88. Tambe M, Pruikkonen S, Mäki Jouppila J, Chen P, Elgaaen B, Straume A, et al. Novel Mad2-targeting miR-493-3p controls mitotic fidelity and cancer cells' sensitivity to paclitaxel. Oncotarget. 2016;7:12267-85 pubmed publisher
    ..We demonstrate miR-493-3p to be a novel negative regulator of mitotic arrest deficient-2 (MAD2), an essential component of the spindle assembly checkpoint that monitors the fidelity of chromosome segregation...
  89. West A, Komives E, Corbett K. Conformational dynamics of the Hop1 HORMA domain reveal a common mechanism with the spindle checkpoint protein Mad2. Nucleic Acids Res. 2017;: pubmed publisher
    ..interaction module found in eukaryotic signaling proteins including the spindle assembly checkpoint protein Mad2 and the meiotic HORMAD proteins...
  90. Li X, Wang D, Zhu Y, Guo R, Wang X, Lubomir K, et al. Mxi1 mutations in human neurofibrosarcomas. Jpn J Cancer Res. 1999;90:740-6 pubmed
    b>Mxi1 is thought to negatively regulate Myc function and may therefore be a potential tumor suppressor gene. Little effort has yet been made to find alterations involving this gene in human solid tumors...
  91. Xu L, Sun Y, Li W, Mai L, Guo Y, Fan Q. MYC and MXI1 protein expression: potential prognostic significance in women with breast cancer in China. Oncol Res Treat. 2014;37:118-23 pubmed publisher
    To investigate the expression levels and the clinical significance of MYC and MXI1 proteins in breast cancer...