MUT

Summary

Gene Symbol: MUT
Description: methylmalonyl CoA mutase
Alias: MCM, methylmalonyl Coenzyme A mutase, methylmalonyl-CoA isomerase, methylmalonyl-CoA mutase, mitochondrial
Species: human

Top Publications

  1. doi Minichromosome maintenance protein expression in benign nevi, dysplastic nevi, melanoma, and cutaneous melanoma metastases
    Alan S Boyd
    Department of Medicine Dermatology, Vanderbilt University, Nashville, Tennessee, USA
    J Am Acad Dermatol 58:750-4. 2008
  2. ncbi Energetics of interaction between the G-protein chaperone, MeaB, and B12-dependent methylmalonyl-CoA mutase
    Dominique Padovani
    Redox Biology Center and the Biochemistry Department, University of Nebraska, Lincoln, NE 68588 0664, USA
    J Biol Chem 281:17838-44. 2006
  3. doi Protection and reactivation of human methylmalonyl-CoA mutase by MMAA protein
    Tóshiko Takahashi-Íñiguez
    Departamento de Biologia Molecular y Biotecnologia, Instituto de Investigaciones Biomedicas, Universidad Nacional Autonoma de Mexico, Mexico, DF 04510, Mexico
    Biochem Biophys Res Commun 404:443-7. 2011
  4. pmc Structures of the human GTPase MMAA and vitamin B12-dependent methylmalonyl-CoA mutase and insight into their complex formation
    D Sean Froese
    Structural Genomics Consortium, University of Oxford OX3 7DU, United Kingdom
    J Biol Chem 285:38204-13. 2010
  5. ncbi Crystal structure and mutagenesis of the metallochaperone MeaB: insight into the causes of methylmalonic aciduria
    Paul A Hubbard
    Department of Chemistry, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    J Biol Chem 282:31308-16. 2007
  6. pmc Loss of allostery and coenzyme B12 delivery by a pathogenic mutation in adenosyltransferase
    Michael Lofgren
    Department of Biological Chemistry, University of Michigan Medical Center, Ann Arbor, Michigan 48109 0600, USA
    Biochemistry 50:5790-8. 2011
  7. pmc Protein kinase C-zeta mediates NF-kappa B activation in human immunodeficiency virus-infected monocytes
    L Folgueira
    Department of Immunology, Mayo Clinic, Rochester, Minnesota 55905, USA
    J Virol 70:223-31. 1996
  8. pmc Liver-directed recombinant adeno-associated viral gene delivery rescues a lethal mouse model of methylmalonic acidemia and provides long-term phenotypic correction
    Nuria Carrillo-Carrasco
    Organic Acid Research Section, Genetics and Molecular Biology Branch, National Human Genome Research Institute, National Institutes of Health Bethesda, MD 20892, USA
    Hum Gene Ther 21:1147-54. 2010
  9. ncbi Regulation of glutathione S-transferase enzymes in primary cultures of rat hepatocytes maintained under various matrix configurations
    E L LeCluyse
    Department of Pharmacology, Toxicology, and Therapeutics, Center for Environmental and Occupational Health, Kansas University Medical Center, Kansas City, Kansas, USA
    Toxicol In Vitro 14:101-15. 2000
  10. ncbi Molecular analysis and heterologous expression of the gene encoding methylmalonyl-coenzyme A mutase from rifamycin SV-producing strain Amycolatopsis mediterranei U32
    W Zhang
    Department of Microbiology, Shanghai Institute of Plant Physiology, Academia Sinica, P R China
    Appl Biochem Biotechnol 82:209-25. 1999

Detail Information

Publications218 found, 100 shown here

  1. doi Minichromosome maintenance protein expression in benign nevi, dysplastic nevi, melanoma, and cutaneous melanoma metastases
    Alan S Boyd
    Department of Medicine Dermatology, Vanderbilt University, Nashville, Tennessee, USA
    J Am Acad Dermatol 58:750-4. 2008
    Minichromosome maintenance (MCM) proteins are a recently elucidated group of polypeptides intimately involved in DNA replication and appreciable only in cycling cells...
  2. ncbi Energetics of interaction between the G-protein chaperone, MeaB, and B12-dependent methylmalonyl-CoA mutase
    Dominique Padovani
    Redox Biology Center and the Biochemistry Department, University of Nebraska, Lincoln, NE 68588 0664, USA
    J Biol Chem 281:17838-44. 2006
    ..These studies provide insights into the energetics of interaction between the radical enzyme methylmalonyl-CoA mutase and MeaB, which are discussed...
  3. doi Protection and reactivation of human methylmalonyl-CoA mutase by MMAA protein
    Tóshiko Takahashi-Íñiguez
    Departamento de Biologia Molecular y Biotecnologia, Instituto de Investigaciones Biomedicas, Universidad Nacional Autonoma de Mexico, Mexico, DF 04510, Mexico
    Biochem Biophys Res Commun 404:443-7. 2011
    ..examined the influence of human MMAA protein on the kinetics of the reaction catalyzed by methylmalonyl-CoA mutase (MCM) by testing both purified recombinant proteins in vitro...
  4. pmc Structures of the human GTPase MMAA and vitamin B12-dependent methylmalonyl-CoA mutase and insight into their complex formation
    D Sean Froese
    Structural Genomics Consortium, University of Oxford OX3 7DU, United Kingdom
    J Biol Chem 285:38204-13. 2010
    ..Cbl) is essential to the function of two human enzymes, methionine synthase (MS) and methylmalonyl-CoA mutase (MUT)...
  5. ncbi Crystal structure and mutagenesis of the metallochaperone MeaB: insight into the causes of methylmalonic aciduria
    Paul A Hubbard
    Department of Chemistry, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    J Biol Chem 282:31308-16. 2007
    ....
  6. pmc Loss of allostery and coenzyme B12 delivery by a pathogenic mutation in adenosyltransferase
    Michael Lofgren
    Department of Biological Chemistry, University of Michigan Medical Center, Ann Arbor, Michigan 48109 0600, USA
    Biochemistry 50:5790-8. 2011
    ..and an escort that delivers the product, adenosylcobalamin (AdoCbl or coenzyme B(12)), to methylmalonyl-CoA mutase (MCM), resulting in holoenzyme formation. Failure to assemble holo-MCM leads to methylmalonic aciduria...
  7. pmc Protein kinase C-zeta mediates NF-kappa B activation in human immunodeficiency virus-infected monocytes
    L Folgueira
    Department of Immunology, Mayo Clinic, Rochester, Minnesota 55905, USA
    J Virol 70:223-31. 1996
    ..In addition, cotransfection of a negative dominant molecule of PKC-zeta (PKC-zeta mut) with NF-kappa B-dependent reporter genes selectively inhibits the HIV- but not phorbol myristate acetate- or ..
  8. pmc Liver-directed recombinant adeno-associated viral gene delivery rescues a lethal mouse model of methylmalonic acidemia and provides long-term phenotypic correction
    Nuria Carrillo-Carrasco
    Organic Acid Research Section, Genetics and Molecular Biology Branch, National Human Genome Research Institute, National Institutes of Health Bethesda, MD 20892, USA
    Hum Gene Ther 21:1147-54. 2010
    ..disorder caused by a deficiency of the ubiquitously expressed mitochondrial enzyme, methylmalonyl-CoA mutase (MUT)...
  9. ncbi Regulation of glutathione S-transferase enzymes in primary cultures of rat hepatocytes maintained under various matrix configurations
    E L LeCluyse
    Department of Pharmacology, Toxicology, and Therapeutics, Center for Environmental and Occupational Health, Kansas University Medical Center, Kansas City, Kansas, USA
    Toxicol In Vitro 14:101-15. 2000
    ..Hepatocytes cultured in serum-free Dulbecco's modified Eagle's medium (DMEM) or modified Chee's medium (MCM) maintained similar levels of total GST protein regardless of the matrix configuration or corresponding cell ..
  10. ncbi Molecular analysis and heterologous expression of the gene encoding methylmalonyl-coenzyme A mutase from rifamycin SV-producing strain Amycolatopsis mediterranei U32
    W Zhang
    Department of Microbiology, Shanghai Institute of Plant Physiology, Academia Sinica, P R China
    Appl Biochem Biotechnol 82:209-25. 1999
    ..A (CoA) into methylmalonyl-CoA, catalyzed by adenosylcobalamin-dependent methylmalonyl-CoA mutase (MCM), represents an important source of building blocks for rifamycin SV biosynthesis...
  11. doi Cobalamin deficiency results in an abnormal increase in L-methylmalonyl-co-enzyme-A mutase expression in rat liver and COS-7 cells
    Motoyuki Nakao
    Department of Applied Biological Chemistry, Osaka Prefecture University, Sakai, Osaka 599 8531, Japan
    Br J Nutr 101:492-8. 2009
    ..study was to examine the effects of cobalamin (Cbl) on the activity and expression of L-methylmalonyl-CoA mutase (MCM) in rat liver and cultured COS-7 cells...
  12. pmc Primary structure and activity of mouse methylmalonyl-CoA mutase
    M F Wilkemeyer
    Howard Hughes Medical Institute, Department of Cell Biology, Baylor College of Medicine, Houston, TX 77030
    Biochem J 271:449-55. 1990
    ..Genetic deficiency of this enzyme in man causes an often fatal disorder of organic acid metabolism termed mut methylmalonicacidaemia...
  13. ncbi Effect of fetuin, a TGFbeta antagonist and pentoxifylline, a cytokine antagonist on hepatic stellate cell function and fibrotic parameters in fibrosis
    Monica Verma-Gandhu
    Department of Pharmacology Faculty of Medicine Dalhousie University, Canada
    Eur J Pharmacol 572:220-7. 2007
    We have previously shown that monocyte conditioned medium (MCM) from patients with liver fibrosis stimulated proliferation of hepatic stellate cells (HSCs), the major cell involved in hepatic fibrosis...
  14. ncbi Initiating DNA synthesis: from recruiting to activating the MCM complex
    M Lei
    Dept of Microbiology and Molecular Genetics, Medical College of Wisconsin, Milwaukee, WI 53226, USA
    J Cell Sci 114:1447-54. 2001
    ..A key component of the pre-RC is the hexameric MCM complex, which is also the presumed helicase of the growing forks...
  15. pmc Genomic structure of murine methylmalonyl-CoA mutase: evidence for genetic and epigenetic mechanisms determining enzyme activity
    M F Wilkemeyer
    Department of Cell Biology, Baylor College of Medicine, Houston, TX 77030
    Biochem J 296:663-70. 1993
    Methylmalonyl-CoA mutase (MCM) is a nuclear-encoded mitochondrial matrix enzyme...
  16. pmc Adenoviral-mediated correction of methylmalonyl-CoA mutase deficiency in murine fibroblasts and human hepatocytes
    Randy J Chandler
    National Human Genome Research Institute, National Institutes of Health, Bethesda, MD 20892, USA
    BMC Med Genet 8:24. 2007
    ..by deficiency of the mitochondrial localized, 5'deoxyadenosylcobalamin dependent enzyme, methylmalonyl-CoA mutase (MUT)...
  17. ncbi Mutation and haplotype analyses of the MUT gene in Japanese patients with methylmalonic acidemia
    Osamu Sakamoto
    Department of Pediatrics, Tohoku University School of Medicine, 1 1 Seiryo machi, Aoba ku, Sendai, 980 8574, Japan
    J Hum Genet 52:48-55. 2007
    ..Apoenzyme-deficient MMA (mut MMA) results from mutations in the nuclear gene MUT...
  18. pmc Adenovirus-mediated gene delivery rescues a neonatal lethal murine model of mut(0) methylmalonic acidemia
    Randy J Chandler
    Genetic Diseases Research Branch, National Human Genome Research Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Hum Gene Ther 19:53-60. 2008
    ..MMA), an autosomal recessive metabolic disorder, is most often caused by mutations in methylmalonyl-CoA mutase (MUT)...
  19. ncbi Cloning, expression and sequence analysis of the genes encoding the heterodimeric methylmalonyl-CoA mutase of Porphyromonas gingivalis W50
    C A Jackson
    Biochemistry and Molecular Biology Unit, School of Dental Science, University of Melbourne, Australia
    Gene 167:127-32. 1995
    Two genes that encode methylmalonyl-CoA mutase (MCM) have been characterised in Porphyromonas gingivalis W50 (Pg)...
  20. ncbi Identification of the human and bovine ATP:Cob(I)alamin adenosyltransferase cDNAs based on complementation of a bacterial mutant
    Nicole A Leal
    Department of Microbiology and Cell Science, University of Florida, Gainesville, Florida 32611, USA
    J Biol Chem 278:9227-34. 2003
    In humans, deficiencies in coenzyme B12-dependent methylmalonyl-CoA mutase (MCM) lead to methylmalonyl aciduria, a rare disease that is often fatal in newborns...
  21. doi Theoretical analysis of the diradical nature of adenosylcobalamin cofactor-tyrosine complex in B12-dependent mutases: inspiring PCET-driven enzymatic catalysis
    Pawel M Kozlowski
    Department of Chemistry, University of Louisville, Louisville, Kentucky 40292, USA
    J Phys Chem B 114:5928-39. 2010
    ..In particular, methylmalonyl-CoA mutase (MCM) and glutamate mutase (GLM) enzymes have been studied; in the case of MCM, the significance of the Y89 residue has ..
  22. ncbi Experimental study on mechanism and rarity of metastases in skeletal muscle
    Chenghua Luo
    Department of General Surgery, 301 Hospital, Beijing 100853, China
    Chin Med J (Engl) 115:1645-9. 2002
    ..To investigate the reasons for the rarity of metastases in skeletal muscle...
  23. pmc Association of germline mutations in the fumarate hydratase gene and uterine fibroids in women with hereditary leiomyomatosis and renal cell cancer
    Laveta Stewart
    Division of Cancer Epidemiology and Genetics, Genetic Epidemiology Branch, National Cancer Institute, National Institutes of Health, Rockville, MD 20892 7231, USA
    Arch Dermatol 144:1584-92. 2008
    ..To investigate the risk of uterine fibroids and other reproductive risk factors in women with hereditary leiomyomatosis and renal cell cancer (HLRCC)...
  24. doi Comparing the General Practice Research Database and the UK Epilepsy and Pregnancy Register as tools for postmarketing teratogen surveillance: anticonvulsants and the risk of major congenital malformations
    Rachel A Charlton
    Department of Pharmacy and Pharmacology, University of Bath, Bath, UK
    Drug Saf 34:157-71. 2011
    ....
  25. ncbi [Couples' knowledge and attitudes regarding the practice of family planning in the jungle of Peru]
    Doris de la Cruz-Peñarán
    Instituto Nacional de Salud Pública INSP, Avenida Universidad 655, colonia Santa María, Ahuacatitlán 62508 Cuernavaca, Morelos, Mexico
    Salud Publica Mex 45:461-71. 2003
    ..To assess the association of knowledge and attitudes about family planning (FP) with its practice in couples of the Peruvian jungle region...
  26. pmc Analysis of genomic instability in Li-Fraumeni fibroblasts with germline p53 mutations
    P K Liu
    Division of Laboratory Medicine, Department of Tumor Biology, The University of Texas MD Anderson Cancer Center, Houston 77030, USA
    Oncogene 12:2267-78. 1996
    ..We used p53(wt/mut) and p53(mut/-) LFS fibroblasts, and p53(-/-) non-LFS cells...
  27. doi Flavin adenine dinucleotide status and the effects of high-dose riboflavin treatment in short-chain acyl-CoA dehydrogenase deficiency
    Bianca T van Maldegem
    Department of Pediatrics, University of Amsterdam, Amsterdam, The Netherlands
    Pediatr Res 67:304-8. 2010
    ..in a prospective open-label cohort study involving 16 patients with SCADD, subdivided into mutation/mutation (mut/mut), mutation/variant (mut/var), and variant/variant (var/var) genotype groups...
  28. pmc Endothelial cell-derived lipase mediates uptake and binding of high-density lipoprotein (HDL) particles and the selective uptake of HDL-associated cholesterol esters independent of its enzymic activity
    Juliane G Strauss
    Institute of Molecular Biology, Biochemistry and Microbiology, University of Graz, Harrachgasse 21, A 8010 Graz, Austria
    Biochem J 368:69-79. 2002
    ..by the finding that, in contrast with EDL, the amount of cell-surface-bound enzymically inactive mutant EDL (MUT-EDL) was not reduced in the presence of HDL and foetal calf serum...
  29. ncbi Long-lasting effects of chronic ozone exposure on rat nasal epithelium
    J R Harkema
    Department of Pathology, College of Veterinary Medicine, Michigan State University, East Lansing, Michigan 48824, USA
    Am J Respir Cell Mol Biol 20:517-29. 1999
    ..We have recently reported that long-term inhalation exposure to ozone causes mucous-cell metaplasia (MCM) in the surface epithelium lining the nasal airways of F344 rats...
  30. ncbi T-->G or T-->A mutation introduced in the branchpoint consensus sequence of intron 4 of lecithin:cholesterol acyltransferase (LCAT) gene: intron retention causing LCAT deficiency
    M Li
    Atherosclerosis Specialty Laboratory, Department of Pathology and Laboratory Medicine, St Paul s Hospital and University of British Columbia, 1081 Burrard Street, Vancouver, British Columbia, Canada
    Biochim Biophys Acta 1391:256-64. 1998
    ..e., LCAT Int-4 MUT-1 (T-->G) and LCAT Int-4 MUT-2 (T-->A)...
  31. ncbi Sorbitol co-feeding reduces metabolic burden caused by the overexpression of a Rhizopus oryzae lipase in Pichia pastoris
    Ramon Ramon
    Departament d Enginyeria Quimica, ETSE, Universitat Autonoma de Barcelona, 08193 Bellaterra, Barcelona, Spain
    J Biotechnol 130:39-46. 2007
    ..Remarkably, a different substrate consumption behaviour was observed depending on the host's phenotype (Mut(+) or Mut(s)) in batch cultures: when the methanol assimilation capacity is genetically reduced (Mut(s) phenotype), ..
  32. doi Involvement of toll-like receptor 4 in alveolar bone loss and glucose homeostasis in experimental periodontitis
    K Watanabe
    Department of Periodontics, College of Dentistry, University of Illinois at Chicago, Chicago, IL 60612 7212, USA
    J Periodontal Res 46:21-30. 2011
    ..We therefore hypothesized that mice with periodontitis and a TLR4 loss-of-function (LOF) mutation fed a high-fat (HF) diet would develop improved glucose homeostasis compared with wild-type (WT) animals with periodontitis fed a HF diet...
  33. ncbi The nuclear envelope prevents reinitiation of replication by regulating the binding of MCM3 to chromatin in Xenopus egg extracts
    M A Madine
    Wellcome CRC Institute, Cambridge, UK
    Curr Biol 5:1270-9. 1995
    A complex of MCM proteins is implicated in ensuring that DNA replicates only once in each cell cycle, by 'replication licensing'...
  34. ncbi Physical and functional interaction between the mini-chromosome maintenance-like DNA helicase and the single-stranded DNA binding protein from the crenarchaeon Sulfolobus solfataricus
    Floriana Carpentieri
    Istituto di Biochimica delle Proteine, Consiglio Nazionale delle Ricerche, Via P Castellino 111, Napoli 80131, Italy
    J Biol Chem 277:12118-27. 2002
    Mini-chromosome Maintenance (MCM) proteins play an essential role in both initiation and elongation phases of DNA replication in Eukarya...
  35. pmc Altered expression of cell cycle and apoptotic proteins in chronic hepatitis C virus infection
    Saira Sarfraz
    1Department of Biological and Biomedical Sciences, Aga Khan University, Karachi, Pakistan
    BMC Microbiol 8:133. 2008
    ..and normal histology (n = 5) were analyzed by immunohistochemistry using antibodies against proliferation marker Mcm-2, G1 phase marker Cyclin D1, S phase marker Cyclin A, cell cycle regulators p21 (CDK inhibitor) and p53 (tumor ..
  36. pmc Virulence variation among isolates of western equine encephalitis virus in an outbred mouse model
    Christopher H Logue
    Division of Vector Borne Infectious Diseases, Centers for Disease Control and Prevention, Fort Collins, CO 80521, USA
    J Gen Virol 90:1848-58. 2009
    ..f.u. of each virus. Two WEEV strains, McMillan (McM) and Imperial 181 (IMP), were the most divergent of the six in genome sequence; McM caused 100 % mortality by 5 ..
  37. ncbi Functional localization and competition between the androgen receptor and T-cell factor for nuclear beta-catenin: a means for inhibition of the Tcf signaling axis
    David J Mulholland
    The Prostate Centre, Jack Bell Research, Vancouver, BC, Canada V6H 3Z6
    Oncogene 22:5602-13. 2003
    ..Transient transfections in SW480 cells (APC(mut/mut)) showed that this mode of repression is functionally independent of APC-mediated beta-catenin ubiquitination...
  38. ncbi [Histologic study of local infiltration of spinal bone giant cell tumor and chordoma]
    Jie Lan
    Department of Orthopedics, Peking University Third Hospital, Beijing 100191, China
    Zhonghua Wai Ke Za Zhi 46:1808-11. 2008
    ..To study the local infiltration length of spinal bone giant cell tumor and chordoma in various para-tumorous tissues...
  39. ncbi Improvement of dissolution rate of piroxicam by inclusion into MCM-41 mesoporous silicate
    V Ambrogi
    Dipartimento di Chimica e Tecnologia del Farmaco, Via del Liceo 1, Perugia, Italy
    Eur J Pharm Sci 32:216-22. 2007
    The aim of the present paper was the use of mesoporous silicate MCM-41 to increase the dissolution rate of piroxicam, a non-steroidal anti-inflammatory drug-class II of the Biopharmaceutic Classification System...
  40. ncbi Modular organization of the Sulfolobus solfataricus mini-chromosome maintenance protein
    Biagio Pucci
    Istituto di Biochimica delle Proteine, Consiglio Nazionale Ricerche, Via P Castellino, 111, 80131 Napoli, Italy
    J Biol Chem 282:12574-82. 2007
    Mini-chromosome maintenance (MCM) proteins form ring-like hexameric complexes that are commonly believed to act as the replicative DNA helicase at the eukaryotic/archaeal DNA replication fork...
  41. ncbi Glucose-6 phosphate dehydrogenase deficiency decreases the vascular response to angiotensin II
    Reiko Matsui
    Vascular Biology Unit, Whitaker Cardiovascular Institute and Evans Department of Medicine, Boston University School of Medicine, Boston, Mass 02118 2393, USA
    Circulation 112:257-63. 2005
    ..We determined the hypertensive and vascular hypertrophic response to Ang II in G6PD-deficient mice...
  42. pmc An enzymatically inactivated hemoglobinase from Necator americanus induces neutralizing antibodies against multiple hookworm species and protects dogs against heterologous hookworm infection
    Mark S Pearson
    Division of Infectious Diseases, Queensland Institute of Medical Research and The Australian Centre for Vaccine Development, Brisbane, Australia
    FASEB J 23:3007-19. 2009
    ..Toward developing a human hookworm vaccine, we expressed both wild-type (Na-APR-1(wt)) and mutant (Na-APR-1(mut)-mutagenesis of the catalytic aspartic acids) forms of Na-APR-1 from the human hookworm, Necator americanus...
  43. doi Methanococcus maripaludis: an archaeon with multiple functional MCM proteins?
    Alison D Walters
    Department of Biology Area 5, PO Box 373, University of York, York YO10 5YW, UK
    Biochem Soc Trans 37:1-6. 2009
    ..Key proteins involved in the initiation and elongation phases of DNA replication include the MCM (minchromosome maintenance) proteins, MCM2-MCM7, a family of six related proteins believed to act as the ..
  44. pmc Cloning and characterization of mouse intestinal MUC3 mucin: 3' sequence contains epidermal-growth-factor-like domains
    L L Shekels
    Department of Medicine, University of Minnesota and VA Medical Center, Minneapolis, MN 55417, USA
    Biochem J 330:1301-8. 1998
    ..We report here the cloning of a mouse caecal mucin (MCM). Amino acid analysis of purified MCM revealed a high content of serine (10.8%) and threonine (25.1%)...
  45. doi Accelerated oxidation of epinephrine by silica nanoparticles
    Zhimin Tao
    Department of Chemistry, Syracuse University, Syracuse, New York 13244, USA
    Langmuir 25:10183-8. 2009
    We have measured the influence of mesoporous silica (MCM-41 and SBA-15) nanoparticles and dense silica nanoparticles on epinephrine oxidation, a pH-dependent reaction, whose rate is small in acidic or neutral solutions but much greater ..
  46. ncbi Study on the indoor volatile organic compound treatment and performance assessment with TiO2/MCM-41 and TiO2/quartz photoreactor under ultraviolet irradiation
    Chia Wei Tsai
    Department of Environmental Engineering, National I Lan University, Taiwan, Republic of China
    J Air Waste Manag Assoc 58:1266-73. 2008
    ..activated by visible irradiation and packed with TiO2/quartz or TiO2/mobile catalytic material number 41 (MCM-41)...
  47. pmc Calcicludine binding to the outer pore of L-type calcium channels is allosterically coupled to dihydropyridine binding
    Xianming Wang
    Department of Cellular and Molecular Physiology, The Penn State Milton S Hershey College of Medicine, Hershey, Pennsylvania 17033, USA
    Biochemistry 46:7590-8. 2007
    ..segment of repeat III were sequentially changed to alanine in groups of three, resulting in the mutant channels, Mut-A, Mut-B, and Mut-C. Mut-A, whose substitutions are proximal to IIIS5, exhibits a 4...
  48. ncbi Immunohistochemical evaluation of metallothionein, Mcm-2 and Ki-67 antigen expression in tumors of the adrenal cortex
    Artur Szajerka
    Second Department of General and Oncological Surgery, Medical University, M Curie Sklodowska Street 66, Poland
    Anticancer Res 28:2959-65. 2008
    The aim of this study was to assess the metallothionein (MT), maintenance protein 2 (Mcm-2) and Ki-67 expressions in adrenocortical adenomas and carcinomas in comparison to normal tissue and evaluate the correlations between these ..
  49. doi Determination of very low levels of gold and palladium in wastewater and soil samples by atomic absorption after preconcentration on modified MCM-48 and MCM-41 silica
    H Ebrahimzadeh
    Department of Chemistry, Shahid Beheshti University, G C, Evin, Tehran, Iran
    Talanta 81:1183-8. 2010
    ..Extraction was investigated on adsorbents prepared by grafting aminopropyl on the surface of MCM-41 and MCM-48 mesoporous silica...
  50. ncbi The influence of flotation solution, sample dilution and the choice of McMaster slide area (volume) on the reliability of the McMaster technique in estimating the faecal egg counts of gastrointestinal strongyles and Dicrocoelium dendriticum in sheep
    G Cringoli
    Dipartimento di Patologia e Sanita Animale, Facolta di Medicina Veterinaria, Universita degli Studi di Napoli Federico II, CREMOPAR Regione Campania, Via Della Veterinaria 1, 80137 Napoli, Italy
    Vet Parasitol 123:121-31. 2004
    ..For each McMaster slide, the GI strongyle and D. dendriticum egg counts were performed under one grid (McM 0.15 ml), two grids (McM 0.3 ml), one chamber (McM 0.5 ml), and both chambers (McM 1.0 ml)...
  51. ncbi The Xenopus origin recognition complex is essential for DNA replication and MCM binding to chromatin
    P Romanowski
    Wellcome CRC Institute, Cambridge
    Curr Biol 6:1416-25. 1996
    The origin recognition complex (ORC) and the minichromosome maintenance (MCM) protein complex were initially discovered in yeast and shown to be essential for DNA replication...
  52. doi Induction of glioma apoptosis by microglia-secreted molecules: The role of nitric oxide and cathepsin B
    So Young Hwang
    Department of Physiology and Biophysics, and Center for Advanced Medical Education by BK21 Project, Inha University, College of Medicine, Nam Ku, Incheon 402 751, Korea
    Biochim Biophys Acta 1793:1656-68. 2009
    ..Microglia conditioned culture medium (MCM) actively stimulated apoptotic death of glioma cells, and the effects of MCM prepared from LPS- or IFN-gamma-..
  53. pmc Specific targeting to B cells by lipid-based nanoparticles conjugated with a novel CD22-ScFv
    Kristin Loomis
    Center for Cancer Research Nanobiology Program, National Cancer Institute at Frederick, National Institutes of Health, Frederick, MD 21702 1201, USA
    Exp Mol Pathol 88:238-49. 2010
    ..Therefore, we generated a mutant of the targeting segment of HA22 (a CD22 scFv) to increase its soluble expression (mut-HA22), and conjugated it to the surface of sonicated liposomes to generate immunoliposomes (mut-HA22-liposomes)...
  54. ncbi Omeprazole treatment of Korean patients: effects on gastric pH and gastrin release in relation to CYP2C19 geno- and phenotypes
    Hyung Keun Roh
    Department of Laboratory Medicine, Division of Clinical Pharmacology, Karolinska Institutet, Huddinge University Hospital, Stockholm, Sweden
    Basic Clin Pharmacol Toxicol 95:112-9. 2004
    ..patients with acid related disease were genotyped for CYP2C19 by allele specific PCR (wt/wt, CYP2C19*1/*1; wt/mut, CYP2C19*1/*2 or *1/*3; mut/mut, CYP2C19*2/*2, *2/*3 or *3/*3)...
  55. pmc A genome-wide screen identifies genes required for formation of the wobble nucleoside 5-methoxycarbonylmethyl-2-thiouridine in Saccharomyces cerevisiae
    Bo Huang
    Department of Molecular Biology, Umea University, 901 87 Umea, Sweden
    RNA 14:2183-94. 2008
    ..tRNA(mcm5s2UUU Lys), and tRNA(mcm5s2UUG Gln) 3' of the wobble nucleoside 5-methoxycarbonylmethyl-2-thiouridine (mcm(5)s(2)U)...
  56. ncbi Photophysical Properties of Lanthanide Hybrids Covalently Bonded To Functionalized MCM-41 by Modified Aromatic Carboxylic Acids
    Ying Li
    Department of Chemistry, Tongji University, Shanghai, 200092, People s Republic of China
    J Fluoresc 19:191-201. 2009
    b>MCM-41 mesoporous silica has been functionalized with aromatic carboxylic acids salicylic acid (Sal) and 2-hydroxyl-3-methylbenzoic acid (HMBA) through co-condensation approach of tetraethoxysilane (TEOS) in the presence of the ..
  57. pmc The Nrf2-inducible antioxidant defense in astrocytes can be both up- and down-regulated by activated microglia:Involvement of p38 MAPK
    Fernando Correa
    Department of Medical Biochemistry and Cell Biology, Institute of Biomedicine, University of Gothenburg, Sweden
    Glia 59:785-99. 2011
    The effects of microglia-conditioned medium (MCM) on the inducible Nrf2 system in astrocyte-rich cultures were investigated by determination of glutathione (GSH) levels, γglutamylcysteine ligase (γGCL) activity, the protein levels of ..
  58. doi Novel and existing mutations in the tyrosine kinase domain of the epidermal growth factor receptor are predictors of optimal resectability in malignant peritoneal mesothelioma
    Jason M Foster
    Division of Surgical Oncology, Creighton University Medical Center, Creighton University, Omaha, NE 68131, USA
    Ann Surg Oncol 16:152-8. 2009
    ..and their tumors were probed for point mutations in the catalytic TK domain of epidermal growth factor receptor (mut+)...
  59. doi Effects of lipophilic components on the compatibility of lipid-based formulations with hard gelatin capsules
    Feng Jing Chen
    Boehringer Ingelheim Pharmaceuticals Inc, 900 Ridgebury Road, Ridgefield, Connecticut 06877, USA
    J Pharm Sci 99:128-41. 2010
    ..1) and an additional lipophilic excipient (Capmul MCM) significantly affected the activity of PG in the fills and the equilibrium of PG between capsule shells and fills...
  60. pmc Immunohistochemical estimation of cell cycle phase in laryngeal neoplasia
    P Chatrath
    MRC Cancer Cell Unit, Hutchison MRC Research Centre, Cambridge CB2 2XZ, UK
    Br J Cancer 95:314-21. 2006
    ..Antibodies against the markers of cell cycle entry, minichromosome maintenance protein-2 (Mcm-2) and Ki67, and putative markers of cell cycle phase, cyclin D1 (G1-phase), cyclin A (S-phase), cyclin B1 (G2-..
  61. ncbi Nuclear envelope alterations in fibroblasts from LGMD1B patients carrying nonsense Y259X heterozygous or homozygous mutation in lamin A/C gene
    Antoine Muchir
    INSERM U582, Institut de Myologie, Groupe Hospitalier Pitie Salpetriere, 75013, Paris, France
    Exp Cell Res 291:352-62. 2003
    ..limb-girdle muscular dystrophy and from her deceased newborn grandchild carrying, respectively, a heterozygous (+/mut) and a homozygous (mut/mut) nonsense Y259X mutation...
  62. pmc Caenorhabditis elegans ABCRNAi transporters interact genetically with rde-2 and mut-7
    Prema Sundaram
    Department of Molecular Biosciences, University of Kansas, Lawrence, Kansas 66045, USA
    Genetics 178:801-14. 2008
    ..Genetic complementation tests reveal functions for ABC(RNAi) transporters in the mut-7/rde-2 branch of the RNAi pathway...
  63. pmc Chimeric alphavirus vaccine candidates protect mice from intranasal challenge with western equine encephalitis virus
    Svetlana Atasheva
    Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, TX 77555, USA
    Vaccine 27:4309-19. 2009
    ..Second-generation vaccine candidates SIN/SIN/McM and SIN/EEE/McM included the envelope glycoprotein genes from WEEV strain McMillan; however, the amino-terminal ..
  64. doi A mammalian cell-based reverse two-hybrid system for functional analysis of 3C viral protease of human enterovirus 71
    Jin Ching Lee
    Division of Biotechnology and Pharmaceutical Research, National Health Research Institutes, Taipei, Taiwan 115, Republic of China
    Anal Biochem 375:115-23. 2008
    ..this system, the GAL4 DNA binding domain (M3) and transactivation domain (VP16) were fused, in-frame, with 3C or 3C(mut). The 3C(mut) was an inactivated protease with mutations at the predicted catalytic triad...
  65. doi MCM2 is a therapeutic target of lovastatin in human non-small cell lung carcinomas
    Xu Zhang
    Department of Stomatology, The Fourth Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang, P R China
    Oncol Rep 33:2599-605. 2015
    ..However, whether or not this effect is mediated through changes in minichromosome maintenance (MCM) 2 expression remains unclear...
  66. ncbi Further studies on using multiple-cross mapping (MCM) to map quantitative trait loci
    Barry Malmanger
    Department of Behavioral Neuroscience, Oregon Health and Science University, Portland, Oregon 97239 3098, USA
    Mamm Genome 17:1193-204. 2006
    ..will be detected in multiple crosses which in turn will provide support for the strategy of multiple-cross mapping (MCM). QTLs with LOD scores greater than 4 were detected on Chrs 1, 2, 3, 8, 9, 13, 14, and 16...
  67. pmc Mutant p53 facilitates somatic cell reprogramming and augments the malignant potential of reprogrammed cells
    Rachel Sarig
    Department of Molecular Cell Biology, Weizmann Institute of Science, Rehovot 76100, Israel
    J Exp Med 207:2127-40. 2010
    ..in human tumors and many mutated forms of p53 gain novel activities, we studied the influence of mutant p53 (mut-p53) on somatic cell reprogramming...
  68. pmc Chromosome association of minichromosome maintenance proteins in Drosophila mitotic cycles
    T T Su
    Department of Biochemistry and Biophysics, University of California San Francisco 94143 0448, USA
    J Cell Biol 139:13-21. 1997
    Minichromosome maintenance (MCM) proteins are essential DNA replication factors conserved among eukaryotes. MCMs cycle between chromatin bound and dissociated states during each cell cycle...
  69. ncbi Wild-type p53 and p73 negatively regulate expression of proliferation related genes
    M J Scian
    Department of Biochemistry and Molecular Biology and the Massey Cancer Center, Virginia Commonwealth University, Richmond, VA 23298, USA
    Oncogene 27:2583-93. 2008
    ..real-time PCR verified the repression of E2F-5, centromere protein A and E, minichromosome maintenance proteins (MCM)-2, -3, -5, -6 and -7 and human CDC25B after p53 expression...
  70. doi Vasopressin regulation of the renal UT-A3 urea transporter
    G S Stewart
    Faculty of Life Sciences, Core Technology Facility, University of Manchester, Manchester, United Kingdom
    Am J Physiol Renal Physiol 296:F642-8. 2009
    ..In this study, we investigated the vasopressin regulation of the basolateral urea transporter UT-A3 using an MDCK-mUT-A3 cell line...
  71. doi Sorptive removal of trinitroglycerin (TNG) from water using nanostructured silica-based materials
    Rabih Saad
    Biotechnology Research Institute, National Research Council of Canada, Montreal, QC, Canada
    J Environ Qual 39:580-6. 2010
    ..The present study describes the use of a nanostructured silica material (Mobil Composite Material no. 48 [MCM-48]) prepared by mixing tetraethylorthosilicate (TEOS) and cetyltrimethylammonium bromide (CTAB) to remove TNG from ..
  72. doi Detailed chemical analysis of regional-scale air pollution in western Portugal using an adapted version of MCM v3.1
    P G Pinho
    Department of Environment, Polytechnic Institute of Viseu, 3504 510 Viseu, Portugal
    Sci Total Environ 407:2024-38. 2009
    A version of the Master Chemical Mechanism (MCM) v3...
  73. ncbi Synthesis of surface modified mesoporous materials via co-assemble route and their drug release properties
    Yi Wang
    School of Chemistry and Chemical Engineering, Yangzhou University, Yangzhou, 225002, Jiangsu, PR China
    J Nanosci Nanotechnol 9:6700-9. 2009
    A series of mesostructured Mn-MCM-41 composites were synthesized via a co-assembly route with tetraethylorthosilicate (TEOS) and manganese sulfate (MnSO4) as silica and manganese sources, respectively...
  74. doi Photocatalytic degradation of isoproturon herbicide over TiO2/Al-MCM-41 composite systems using solar light
    M V Phanikrishna Sharma
    Inorganic and Physical Chemistry Division, Indian Institute of Chemical Technology, Hyderabad 500 607, India
    Chemosphere 72:644-51. 2008
    ..investigation covers immobilization of TiO2 using a simple solid state dispersion technique over mesoporous Al-MCM-41 support for the treatment of isoproturon herbicide...
  75. pmc Differential arrival of leading and lagging strand DNA polymerases at fission yeast telomeres
    Bettina A Moser
    Department of Biochemistry and Molecular Genetics, University of Illinois at Chicago, Chicago, IL 60607, USA
    EMBO J 28:810-20. 2009
    ..telomere-specific proteins (telomerase Trt1, Taz1, Rap1, Pot1 and Stn1), DNA replication proteins (DNA polymerases, MCM, RPA), checkpoint protein Rad26 and DNA repair protein Nbs1 to telomeres...
  76. doi Controlled release of vitamin B2 using mesoporous materials functionalized with amine-bearing gate-like scaffoldings
    Andrea Bernardos
    Instituto de Quimica Molecular Aplicada, Departamento de Quimica, Universidad Politecnica de Valencia, Valencia, Spain
    J Control Release 131:181-9. 2008
    ..To obtain solid S1 the mesoporous MCM-41 support was first synthesized using tetraethyl orthosilicate (TEOS) as hydrolytic inorganic precursor and the ..
  77. pmc Vaccination with an attenuated strain of Francisella novicida prevents T-cell depletion and protects mice infected with the wild-type strain from severe sepsis
    Jyotika Sharma
    South Texas Center for Emerging Diseases and Department of Biology, University of Texas at San Antonio, One UTSA Circle, San Antonio, TX 78249 1644, USA
    Infect Immun 77:4314-26. 2009
    ..Intranasal infection with an attenuated mutant (Mut) of F...
  78. ncbi Antagonistic effect of electromagnetic field exposure on coccidiosis infection in broiler chickens
    M A Elmusharaf
    Department of Nutrition, Faculty of Veterinary Medicine, Utrecht University, The Netherlands
    Poult Sci 86:2139-43. 2007
    ..In the cages of EMF-treated birds, a field strength of 5 muT root mean square was created for a period of 30 min/d...
  79. doi Cloning and expression of functional full-length human tissue plasminogen activator in Pichia pastoris
    Keivan Majidzadeh-A
    Biotechnology Department, Pasteur Institute of Iran, Tehran, Iran
    Appl Biochem Biotechnol 162:2037-48. 2010
    ..The methylotrophic yeast, Pichia pastoris GS115 strain, was transformed with this cassette, and methanol utilizing (mut+) transformants were selected for production and secretion of human t-PA into culture media...
  80. pmc Assessing the accuracy of quantitative molecular microbial profiling
    Denise M O'Sullivan
    Molecular Biology, LGC Ltd, Queens Road, Teddington TW11 0LY, UK
    Int J Mol Sci 15:21476-91. 2014
    ..We previously developed a metagenomic control material (MCM) to investigate error when performing different sequencing strategies...
  81. pmc Coupling mutagenesis and parallel deep sequencing to probe essential residues in a genome or gene
    William P Robins
    Department of Microbiology and Immunobiology, Harvard Medical School, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 110:E848-57. 2013
    ..Here we describe an approach called Mut-seq, which allows the identification of virtually all of the essential residues present in a whole genome through ..
  82. pmc Rsf-1, a chromatin remodelling protein, interacts with cyclin E1 and promotes tumour development
    Jim Jinn Chyuan Sheu
    Department of Pathology, Gynecology and Obstetrics and Oncology, Johns Hopkins Medical Institutions, Baltimore, MD 21231, USA
    J Pathol 229:559-68. 2013
    ..Ectopic expression of Rsf-1 and cyclin E1 in non-tumourigenic TP53(mut) RK3E cells led to an increase in cellular proliferation and tumour formation by activating cyclin E1-associated ..
  83. ncbi Suppressors of Escherichia coli mutT: antimutators for DNA replication errors
    R M Schaaper
    Laboratory of Molecular Genetics, National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709, USA
    Mutat Res 350:17-23. 1996
    ..8-oxoG) transversion mispairs and A.C or T.G transition mispairs. The finding may also have significance for teh interpretation of the antimutator effect conferred by these dnaE alleles in a wild-type (mut+) background.
  84. doi Quick photo-Fenton degradation of phenolic compounds by Cu/Al2O3-MCM-41 under visible light irradiation: small particle size, stabilization of copper, easy reducibility of Cu and visible light active material
    Amaresh C Pradhan
    Colloids and Material Chemistry Department, Institute of Minerals and Materials Technology CSIR, Bhubaneswar 751013, Orissa, India
    Dalton Trans 42:558-66. 2013
    ..phenol, 2-chloro-4-nitrophenol and 4-chloro-2-nitrophenol) in aqueous solution using mesoporous Cu/Al(2)O(3)-MCM-41 nanocomposite as a heterogeneous photo-Fenton-like catalyst...
  85. doi The MCART Consortium animal models series
    Thomas J MacVittie
    Department of Radiation Oncology, University of Maryland, School of Medicine, Baltimore, MD, USA
    Health Phys 103:340-2. 2012
    ..Medical Countermeasures Against Radiological Threats (MCART), is the development of medical countermeasures (MCM) to treat the key sequelae of the acute radiation syndrome (ARS) and the delayed effects of acute radiation ..
  86. doi Developmental competence and embryo quality of small oocytes from pre-pubertal goats cultured in IVM medium supplemented with low level of hormones, insulin-transferrin-selenium and ascorbic acid
    S Hammami
    Facultat de Veterinaria, Universitat Autonoma de Barcelona, Bellaterra, Spain
    Reprod Domest Anim 48:339-44. 2013
    ..media: conventional IVM medium (CM), growth medium (GM: CM+ITS+AA and low level of hormones), modified CM (mCM: CM with low level of hormones) and modified GM (mGM: CM+ITS+AA and normal level of hormones)...
  87. pmc Multiple domains of fission yeast Cdc19p (MCM2) are required for its association with the core MCM complex
    D A Sherman
    Molecular Biology and Virology Laboratory, The Salk Institute, La Jolla, California 92037, USA
    Mol Biol Cell 9:1833-45. 1998
    The members of the MCM protein family are essential eukaryotic DNA replication factors that form a six-member protein complex...
  88. ncbi Gastrointestinal expression and partial cDNA cloning of murine Muc2
    B J Van Klinken
    Emma Children s Hospital, Academic Medical Center, 1105 AZ Amsterdam, The Netherlands
    Am J Physiol 276:G115-24. 1999
    ..of mucin in the protection of the colonic epithelium in the mouse, we aimed to identify the murine colonic mucin (MCM) and its encoding gene...
  89. ncbi Catalytic pyrolysis of waste mandarin over nanoporous materials
    Young Kwon Park
    Graduate School of Energy and Environmental Engineering, University of Seoul, Seoul 130 743, Korea
    J Nanosci Nanotechnol 13:718-21. 2013
    Catalytic pyrolysis of waste mandarin was performed using nanoporous catalysts. AI-MCM-41 and Meso-MFI, which had different acid characteristics, were used...
  90. doi Synthesis of mesoporous hollow silica nanospheres using polymeric micelles as template and their application as a drug-delivery carrier
    Manickam Sasidharan
    Department of Chemistry, Faculty of Science and Engineering, Saga University, 1 Honjo Machi, Saga 840 8502, Japan
    Dalton Trans 42:13381-9. 2013
    ..The hollow silica nanospheres exhibited higher storage capacity than the well-known mesoporous silica MCM-41...
  91. doi MCM-41 as a useful vector for rutin topical formulations: synthesis, characterization and testing
    Gloria Berlier
    Universita di Torino, Dipartimento di Chimica and NIS, Nanostructured Interfaces and Surfaces Centre of Excellence, Via Giuria 7, 10125 Torino, Italy
    Int J Pharm 457:177-86. 2013
    ..These issues were addressed by preparing, characterizing and testing rutin inclusion complexes with MCM-41 mesoporous silica...
  92. doi Multifunctional enveloped mesoporous silica nanoparticles for subcellular co-delivery of drug and therapeutic peptide
    Guo Feng Luo
    Key Laboratory of Biomedical Polymers of Ministry of Education and Department of Chemistry, Wuhan University, Wuhan 430072, P R China
    Sci Rep 4:6064. 2014
    ..Mesoporous silica MCM-41 nanoparticles were used as the core for loading antineoplastic drug topotecan (TPT)...
  93. pmc Analysis of the crystal structure of an active MCM hexamer
    Justin M Miller
    Department of Structural Biology, St Jude Children s Research Hospital, Memphis, United States
    elife 3:e03433. 2014
    In a previous Research article (Froelich et al., 2014), we suggested an MCM helicase activation mechanism, but were limited in discussing the ATPase domain because it was absent from the crystal structure...
  94. doi Dual stimuli-responsive nano-vehicles for controlled drug delivery: mesoporous silica nanoparticles end-capped with natural chitosan
    Abdul Hakeem
    Key Laboratory for Large Format Battery Materials and Systems, Ministry of Education, School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology, Wuhan 430074, China
    Chem Commun (Camb) 50:13268-71. 2014
    Herein, we report natural chitosan end-capped MCM-41 type MSNPs as novel, dual stimuli, responsive nano-vehicles for controlled anticancer drug delivery...
  95. pmc DNA binding polarity, dimerization, and ATPase ring remodeling in the CMG helicase of the eukaryotic replisome
    Alessandro Costa
    London Research Institute, Cancer Research UK, London, United Kingdom
    elife 3:e03273. 2014
    ..The structure shows that the MCM subunits of the CMG bind preferentially to single-stranded DNA, establishes the polarity by which DNA enters into ..
  96. pmc Origin licensing requires ATP binding and hydrolysis by the MCM replicative helicase
    Gideon Coster
    Cancer Research UK London Research Institute, Clare Hall Laboratories, South Mimms, Herts EN6 3LD, UK
    Mol Cell 55:666-77. 2014
    Loading of the six related Minichromosome Maintenance (MCM) proteins as head-to-head double hexamers during DNA replication origin licensing is crucial for ensuring once-per-cell-cycle DNA replication in eukaryotic cells...
  97. doi Single cell in situ detection and quantification of metal oxide nanoparticles using multimodal correlative microscopy
    Quentin Le Trequesser
    Universite de Bordeaux, Centre d Etudes Nucléaires Bordeaux Gradignan CENBG, Chemin du Solarium, 33175 Gradignan, France
    Anal Chem 86:7311-9. 2014
    ..and above all quantify NPs in a single cell, we propose an approach based on a multimodal correlative microscopy (MCM), via the complementarity of three imaging techniques: fluorescence microscopy (FM), scanning electron microscopy (..
  98. doi Peculiar behavior of MWW materials in aldol condensation of furfural and acetone
    Oleg Kikhtyanin
    Research Institute of Inorganic Chemistry, RENTECH UniCRE, Chempark Litvínov, Záluží Litvínov, 436 70, Czech Republic
    Dalton Trans 43:10628-41. 2014
    MWW family of different structural types (MCM-22, MCM-49, MCM-56 and MCM-36) was used as catalysts for aldol condensation of furfural and acetone studied in a batch reactor at 100 °C, autogenous pressure and a reaction time of 0-4 h...
  99. pmc Mitochondria-targeted Ogg1 and aconitase-2 prevent oxidant-induced mitochondrial DNA damage in alveolar epithelial cells
    Seok Jo Kim
    From the Department of Medicine, Division of Pulmonary and Critical Care Medicine, Jesse Brown Veterans Affairs Medical Center and Northwestern University Feinberg School of Medicine, Chicago, Illinois 60611
    J Biol Chem 289:6165-76. 2014
    ..Overexpression of wild-type mt-hOgg1 or mt-long α/β 317-323 hOgg1 mutant incapable of DNA repair (mt-hOgg1-Mut) each blocked A549 cell oxidant-induced mtDNA damage, mitochondrial p53 translocation, and intrinsic apoptosis as ..
  100. ncbi A DNA helicase activity is associated with an MCM4, -6, and -7 protein complex
    Y Ishimi
    Mitsubishi Kasei Institute of Life Sciences, 11 Minamiooya, Machida, Tokyo 194, Japan
    J Biol Chem 272:24508-13. 1997
    All six minichromosome maintenance (MCM) proteins have DNA-dependent ATPase motifs in the central domain which is conserved from yeast to mammals...
  101. doi Study of catalytic reduction and photodegradation of methylene blue by heterogeneous catalyst
    Sh Sohrabnezhad
    Department of Chemistry, Faculty of Science, University of Mohaghegh Ardabili, Ardabil, Iran
    Spectrochim Acta A Mol Biomol Spectrosc 81:228-35. 2011
    The photocatalytic degradation of methylene blue is investigated in aqueous solution containing CoS/nanoAl-MCM-41 photocatalyst under visible light...

Research Grants63

  1. Understanding the Biological Function of MCM 10
    Anja Katrin Bielinsky; Fiscal Year: 2013
    ..Lessons learnt from simple organisms will be applied to the human system to understand the molecular pathways of cancer. ..
  2. Regulation of RPA Activity in DNA Repair
    James A Borowiec; Fiscal Year: 2012
    ..RPA selectively associates with the replication fork, by characterization of its interaction with the MCM complex...
  3. Genetic Analysis of MSM Protein Function in S. Pombe
    Susan L Forsburg; Fiscal Year: 2012
    ..Dysregulation of the genes encoding the conserved MCM helicase occurs in cancer cells, and mcm mutations are also linked to cancer development in mice...
  4. The molecular basis of RECQ4-associated genetic disorders and cancer predispositi
    Yilun Liu; Fiscal Year: 2013
    ..Our studies allow us to conclude that RECQ4 is an integral component of the MCM replicative helicase complex participating in DNA replication in human cells...
  5. GENETIC AND MOLECULAR ANALYSIS OF YEAST DNA REPLICATION
    Robert A Sclafani; Fiscal Year: 2012
    ..Three specific aims are proposed. In aim #1, we will elucidate the function and regulation of the MCM DNA helicase by investigating both the N- terminal and C- terminal [unreadable] (Beta)-fingers and the N-terminal ..
  6. Linking DNA Replication Origin Licensing with Cell Cycle Progression
    Kate E Coleman; Fiscal Year: 2013
    ..replication in S phase by the chromatin-loading of a DNA helicase known as the Mini-Chromosome Maintenance Complex (MCM) during G1 phase of the cell cycle...
  7. Regulation of MCM helicase loading and activation in the model system Saccharomyc
    Rebecca Ferguson; Fiscal Year: 2013
    ..The long-term objective of this proposal is to understand the regulation of MCM helicase function for eukaryotic chromosomal DNA replication using the model system Saccharomyces cerevisiae...
  8. The Role of the MCM2-7 Complex in the Replication Fork Processivity
    Anthony Schwacha; Fiscal Year: 2013
    ..As one of the few factors essential in both the initiation and elongation phases of DNA replication, defects in MCM activity can compromise the precision of DNA replication in multiple ways...
  9. Functional analysis of Chk2 in DNA damage response
    Jun Hyun Kim; Fiscal Year: 2013
    ..large-scale phosphoproteomic screening has resulted in the finding of new Chk2 targets, minichromosome maintenance (MCM) that might be associated with Chk2 functions in S-phase...
  10. The Structural Basis of Eukaryotic Replication Origin Licensing by Cryo-EM
    Huilin Li; Fiscal Year: 2010
    ..structural feature in the ORC-Cdc6p complex that is similar in size to the presumptive replicative hexameric MCM helicase...
  11. Replication Initiation in Bacteria and Eukaryotes
    David Jeruzalmi; Fiscal Year: 2012
    ..Aim #3 exploits our recent identification of a unique bacterial MCM complex, which is more tractable, but should still provide a faithful model for its eukaryotic counterparts...
  12. The Regulation of DNA Replication Kinetics
    NICHOLAS R RHIND; Fiscal Year: 2013
    ..We propose that the probability of origin firing is regulated by the number of MCM complexes - the replicative helicase which establishes an origin as a site of replication initiation - loaded ..
  13. Chromatin Remodeling by Cdt1: Role in DNA Replication and Tumorigenesis
    Mark G Alexandrow; Fiscal Year: 2013
    ..Cdt1 stimulates the loading of Mini- Chromosome Maintenance (MCM) proteins at preRCs, and Geminin blocks this effect, but the molecular mechanisms behind this are unknown...
  14. DNA-PROTEIN INTERACTIONS AT THE REPLICATION FORK
    Jack D Griffith; Fiscal Year: 2012
    ..attached to magnetic particles will be incubated with yeast cell extracts to load the pre-RC complex including Mcm and Cdc6 factors. Their structure will be probed by Western analysis and EM using nanoscale 'biopointers'...
  15. Role of Histone H2B Ubiquitylation in DNA Replication
    KELLY MIGUEL TRUJILLO; Fiscal Year: 2013
    ..I have discovered that the MCM helicase falls off the template in htb-K123R cells that cannot be ubiquitylated at the G1-S phase transition...
  16. Structural Studies of MCM Complex
    Xiaojiang S Chen; Fiscal Year: 2012
    Project Summary: Structural studies of MCM complex Minichromosome maintenance protein (MCM) complex functions as a helicase that unwinds DNA to provide template for DNA replication and plays a critical role in regulating cell growth...
  17. Molecular mechanisms of DNA replication
    ERIC ENEMARK; Fiscal Year: 2013
    ..In human and other eukaryotic cells, the helicase engine is the hexameric MCM complex. The mechanisms that MCMs use to operate upon DNA are poorly understood at the molecular level...
  18. Structural Basis of Large T Helicase Function in SV40 DNA Replication
    Xiaojiang S Chen; Fiscal Year: 2013
    ..the helicase and cellular initiator proteins that consist of multiple initiator factors (such as Orc, Ctd1, Cdc6, MCM in eukaryotes, or DnaA/DnaC/DnaB in prokaryotes) to initiate DNA replication, i.e...
  19. MCM PROTEIN FUNCTION DURING EUKARYOTIC DNA REPLICATION
    Stephen Bell; Fiscal Year: 2002
    ..The MCM proteins are a family of six related proteins that play a central role in the replication process in the yeast S...
  20. Role of Ddk Kinase in Regulation of DNA Replication
    Wei Jiang; Fiscal Year: 2006
    ..We will examine whether HsCdc7/HsDbf4 phosphorylation of MCM2 affects the formation or conformation of MCM heteromeric complexes and regulates the helicase activity of MCM complex(es)...
  21. Role of GLIS2 in the Development of Cystic Kidney Disease
    Massimo Attanasio; Fiscal Year: 2013
    ..the effect of the inhibition of TLR-2/NF-?B signaling on the inflammatory infiltration and fibrosis in Glis2mut/mut mice kidneys by generating Tlr2-/-;Glis2mut/mut and Myd88flox/flox;Glis2mut/mut;KspCre double mutants and ..
  22. FANCD2 interaction with mismatch repair proteins and MCM2-7
    Gary M Kupfer; Fiscal Year: 2013
    ..Our data indicate that binding to a subset of the MCM subunits results in inhibition of replication complex assembly...
  23. Nanotheranostics for Early Colorectal Cancer Detection and Treatment
    Jeffrey S Souris; Fiscal Year: 2013
    ..invasive M-T staging of polyp/neoplastic disease in situ, we will synthesize and tri-functionalize biocompatible MCM-41 mesoporous silica nanoparticles (MSNs) for the targeted, endoscopically-traceable, delivery of conventional ..
  24. INBORN ERRORS--MOLECULAR ANALYSIS IN CULTURED CELLS
    Leon Rosenberg; Fiscal Year: 1990
    ..to cystathionine beta-synthase (CS) deficiency; methylmalonic acidemia, due to methylmalonyl CoA mutase apoenzyme (MUT) deficiency; hyperammonemia, due to ornithine transcarbamylase (OTC) deficiency; and propionic acidemia, due to ..
  25. Regulation of V(D)J recombination by Rag2 C terminus
    Patricia Cortes; Fiscal Year: 2013
    ..will address these issues while extending our findings that showed interaction of Rag2 with core histones and the MCM complex...
  26. VEGF and Skeletal Muscle Adaptation during Chronic Overload
    KIMBERLY ANN HUEY; Fiscal Year: 2011
    ..or mice overexpressing wild type Hsp27 (human form of Hsp25) (hspTG) or a non-phosphorylatable Hsp27 mutant (mut-hspTG), we will test the hypothesis that (p)Hsp25 is a critical mediator of the angiogenic, myogenic and/or anti-..
  27. Antisense oligonucleotide treatment for myotonic dystrophy
    Charles A Thornton; Fiscal Year: 2013
    ..end, we are proposing to develop RNase H-active antisense oligonucleotides (ASOs) targeting the mutant human DMPK {mut-hDMPK) mRNA...
  28. Resolution of Epithelial Cell Hyperplasia
    Yohannes Tesfaigzi; Fiscal Year: 2013
    ..Epithelial cell hyperplasia in response to an inflammatory response is accompanied by mucous cell metaplasia (MCM), the appearance of mucous cells in airways that are normally devoid of these cells...
  29. Mechanisms of CDX2 regulation of the IGF axis
    Duyen Dang; Fiscal Year: 2013
    ..Conversely, in colon cancers with MUT-p53, CDX2 has the opposite effect...
  30. KL4 Surfactant to Mitigate Radiation-Induced Lung Injury
    Robert Segal; Fiscal Year: 2013
    ..is evaluating its proprietary peptide-based synthetic KL4 surfactant (lucinactant) as a broad-spectrum, multi-use MCM against chemical, biological, radiological and nuclear threat agents targeting the lung...
  31. Error Correction in DNA Synthesis: A Biochemical Study
    MYRON GOODMAN; Fiscal Year: 2013
    ..to transfer a molecule of RecA7ATP from its 3'- end to convert inactive pol V into mutagenically active pol V Mut. The properties of pol V Mut (UmuD'2C- RecA7ATP) are regulated through a biochemical cycle of polymerase ..
  32. Regulation of mucous cell metaplasia in asthma
    Yohannes Tesfaigzi; Fiscal Year: 2012
    ..This phenotype is called mucous cell metaplasia (MCM)...
  33. MOLECULAR GENETIC STUDIES ON METHYLMALONYL COA MUTASE
    Fred Ledley; Fiscal Year: 1992
    ..clone for methylmalonyl CoA mutase from human liver and demonstrated that this clone can be used to transduce MCM activity into cultured cells by genetic transfer...
  34. Defining the importance of CD8+ T Cell Breadth in SIV/HIV protective immunity
    DAVID H O'CONNOR; Fiscal Year: 2013
    ..We have discovered that Mauritian cynomolgus macaques (MCM) infected with simian immunodeficiency virus (SIV), a virus that causes the same disease as HIV causes in humans, ..
  35. The Role of Osteoactivin in Osteoblast Development and Function
    Fayez F Safadi; Fiscal Year: 2013
    ..During the previous funding period and since the last submission, we established colonies of OA KO and OA mut. mice, and also generated transgenic (Tg) mice that over-express OA in bone. Preliminary data from OA KO, OA mut...
  36. GABA(A) Receptor Assembly/Trafficking/Function and Epilepsy Missense Mutations
    ROBERT LOUDEN MACDONALD; Fiscal Year: 2013
    ..to a dominant negative effect of the mutant subunit, 2) the mutation alters cortical and thalamic inhibition, 3) mut [unreadable]3 (P11S) subunits are reduced in mouse brain, and 4) the mutation causes altered transcriptional ..
  37. Optimization of SWCNT/siRNA complex formulation for tumor accumulation
    Lynn Kirkpatrick; Fiscal Year: 2012
    ..Mutant KRAS (mut-KRAS) is the prototypical undruggable cancer target...
  38. BIOCHEMICAL BASIS OF SOS-INDUCED MUTAGENESIS
    MYRON GOODMAN; Fiscal Year: 2013
    ..is to transfer a molecule of RecA-ATP from its 32-end to convert inactive Pol V into mutagenically active Pol V Mut (UmuD22C-RecA-ATP)...
  39. Abstinence Reinforcement Therapy (ART) for Rural Veteran Smokers
    Patrick S Calhoun; Fiscal Year: 2013
    ..TC), a tele-medicine clinic for access to nicotine replacement (NRT), and mobile contingency management (mCM) or a control condition (i.e...
  40. Optimizing Genetic Testing for Deafness for Clinical Diagnostics
    Richard J H Smith; Fiscal Year: 2013
    ..e. low frequency hearing loss as seen with WFS1) (Hilgert et al Mut Res 2009)...
  41. MDEpiNet: MCM (Medical Countermeasures)
    Art Sedrakyan; Fiscal Year: 2012
    Summary This application proposes creation of a major innovative initiative/program on medical countermeasures (MCM) within the Medical Device Epidemiology Network (MDEpiNet)...
  42. Meiotic recombination in Drosophila
    JEFF JOSEPH SEKELSKY; Fiscal Year: 2013
    ..To better understand crossover control, we will test hypotheses for functions of a set of genes that encode MCM-like proteins essential for crossover formation, using a combination of genetic, biochemical, and cytological ..
  43. Treatment of Colitis using a Novel CXCR3 Biologic Antagonist
    James W Lillard; Fiscal Year: 2013
    ..b CXCR3 interactions, JYANT Technologies has developed a novel mutant CXCR3 ligand-immunoglobulin fusion protein (mut-CXCL11-Ig) that lacks immunogenic, glycosaminoglycan (connective tissues)-binding sites but retains the ability to ..
  44. 2D Silicon Transducer Compression Plates for Breast Ultrasound
    KAI ERIK THOMENIUS; Fiscal Year: 2010
    ..the development of a novel breast imaging device based on the use of silicon micro-machined ultrasound transducer (MUT) arrays placed on both upper and lower compression plates in a manner similar to mammography...
  45. THE MDEPINET MEDICAL COUNTER MEASURES STUDY
    Frederic S Resnic; Fiscal Year: 2012
    ..Because medical countermeasures (MCM) devices require rapid assessment and approval to ensure an effective public health response in the event of a ..
  46. Stress Signaling Pathways and Resistance in Colorectal Cancer
    Scott Kopetz; Fiscal Year: 2013
    ..and resistance to targeted therapy for CRC;and 3) conduct clinical trials of inhibitors of AKT, PI3K, and mut-KRAS signaling in patients with advanced CRC using patient tumor biopsy results to identify potential biomarkers ..
  47. Inhibiting oncogenic KRAS for cancer therapy
    Garth Powis; Fiscal Year: 2013
    ..Mutant KRAS (mut-KRAS) is the predominant oncogenic form of RAS, and is present in 17-25% of all human cancers where it plays a ..
  48. MCM Helicase as a Novel Target for Pancreatic Cancer Treatment
    Mark G Alexandrow; Fiscal Year: 2012
    ..The goal of this proposal is to explore the novel possibility that co-suppression of the replicative MCM helicase complex can enhance the anti-proliferative effects of chemotherapeutic agents used in pancreatic cancer ..
  49. RNA-Mediated Silencing: Mechanisms and Biological Roles in Chlamydomonas
    Heriberto Cerutti; Fiscal Year: 2009
    ..1) Molecular characterization of mutants defective in dsRNA-mediated silencing (Mut-68, Mut-70, and Mut-91)...
  50. Molecular interactions and restoration strategies of PTEN and p53 in gliomas
    Roger Abounader; Fiscal Year: 2013
    ..tumor promoting properties by enhancing the half-life and oncogenic effects of gain-of-function p53 mutants (mut-p53)...
  51. FACTORS REGULATING TRANSPOSITION IN C. ELEGANS
    John Collins; Fiscal Year: 1992
    ..activity of Tc3, Tc4, and Tc5 has been detected only in genetic backgrounds containing the mutator mutation mut-2(r459). Genetic methods will be used to determine. if the activity of these elements is indeed mut-2-specific...
  52. Targeting mutant KRAS for cancer therapy
    Lynn Kirkpatrick; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): Mutant KRAS (mut-KRAS) is the prototypical undruggable cancer target...
  53. Role of ATM in Radiosensitivity of Gliomas
    Chandan Guha; Fiscal Year: 2004
    ..t.) and U-138 (p53 mut) GBM cells resulting in enhanced radiosensitivity in vitro...
  54. ENDOTOXIN INDUCED MUCOUS CELL METAPLASIA
    Yohannes Tesfaigzi; Fiscal Year: 2003
    ..that Bcl-2 expression (a gene that inhibits apoptosis or programmed cell death) plays in mucous cell metaplasia (MCM)...
  55. Defining non-classical, CMV-induced CD8 T cell responses
    Jonah B Sacha; Fiscal Year: 2013
    ..In contrast, Mauritian cynomolgus macaques (MCM), descendants from a population bottleneck event, exhibit simplified MHC genetics with only seven completely ..
  56. Canine model for assessing CNS access P-gp substrates
    KATRINA MEALEY; Fiscal Year: 2005
    The overall goal of the proposed research is to validate a P-glycoprotein (P-gp) deficient canine model [MDR1(mut/mut)] for delineating the role of P-gp in restricting access of therapeutic agents to the central nervous system (CNS)...
  57. PROTEIN PHOSPHATASE 2A AND TUMOR ANTIGENS IN CELL GROWTH
    Gernot Walter; Fiscal Year: 2004
    ..The main focus will be on identifying the substrate(s) of PP2A. Potential candidates are ORC, cdc6, MCM, cdc45, RPA, and DNA polymerase alpha, known to be involved in initiation...
  58. REGULATION OF MISMATCH REPAIR IN A MODEL E COLI SYSTEM
    ALICIA DOMBROSKI; Fiscal Year: 2001
    DESCRIPTION: The long-term goal of this project is to understand the regulatio of the mut S, mutL, and mut H DNA repair genes and the possible implications o this regulation to DNA repair, spontaneous and induced mutagenesis, and ..
  59. FISSION YEAST DNA REPLICATION ORGINS
    Joel Huberman; Fiscal Year: 2001
    ..ORC binding appears necessary for the subsequent binding of Cdc6p, the Mcm family of proteins, and perhaps other proteins at the beginning of G1 phase...
  60. EFFECTS OF 03/ENDOTOXIN COEXPOSURE ON NASAL EPITHELIUM
    James Wagner; Fiscal Year: 2000
    ..Ozone exposure causes inflammation that is followed by mucous cell metaplasia (MCM) which is the appearance of mucous secreting cells in regions of nasal epithelium normally devoid of secretory ..
  61. MOOD AND MEMORY
    JOHN KIHLSTROM; Fiscal Year: 1990
    ..autobiographical memory designs to explore the phenomena of mood- dependent memory (MDM) and mood-congruent memory (MCM) and resource allOcation effects...
  62. Development of Medical Countermeasures to Mitigate or Treat GI-ARS
    THOMAS RIVENBURG; Fiscal Year: 2013
    The contracts awarded under this initiative will advance the development of candidate medical countermeasures (MCM) that can mitigate or treat gastrointestinal (GI) syndrome of the acute radiation syndrome (GI-ARS) and increase survival...