Gene Symbol: MUS81
Description: MUS81 structure-specific endonuclease subunit
Alias: SLX3, crossover junction endonuclease MUS81, MUS81 endonuclease homolog, SLX3 structure-specific endonuclease subunit homolog
Species: human
Products:     MUS81

Top Publications

  1. Chen X, Melchionna R, Denis C, Gaillard P, Blasina A, Van de Weyer I, et al. Human Mus81-associated endonuclease cleaves Holliday junctions in vitro. Mol Cell. 2001;8:1117-27 pubmed
    b>Mus81, a protein with homology to the XPF subunit of the ERCC1-XPF endonuclease, is important for replicational stress tolerance in both budding and fission yeast...
  2. Kim Y, Spitz G, Veturi U, Lach F, Auerbach A, Smogorzewska A. Regulation of multiple DNA repair pathways by the Fanconi anemia protein SLX4. Blood. 2013;121:54-63 pubmed publisher
    ..It interacts with and enhances the activity of XPF-ERCC1, MUS81-EME1, and SLX1 nucleases, but the requirement for the specific nucleases in SLX4 function is unclear...
  3. Oğrünç M, Sancar A. Identification and characterization of human MUS81-MMS4 structure-specific endonuclease. J Biol Chem. 2003;278:21715-20 pubmed
    ..Recently, a novel nuclease involved in resolving Holliday junction-like structures, Mus81, has been reported in yeast and humans...
  4. Munoz I, Hain K, Déclais A, Gardiner M, Toh G, Sanchez Pulido L, et al. Coordination of structure-specific nucleases by human SLX4/BTBD12 is required for DNA repair. Mol Cell. 2009;35:116-27 pubmed publisher
    ..Here we describe the identification of human SLX4, a scaffold for DNA repair nucleases XPF-ERCC1, MUS81-EME1, and SLX1...
  5. Wu F, Shirahata A, Sakuraba K, Kitamura Y, Goto T, Saito M, et al. Downregulation of Mus81 as a novel prognostic biomarker for patients with colorectal carcinoma. Cancer Sci. 2011;102:472-7 pubmed publisher
    The Mus81 gene encodes a critical endonuclease involved in DNA repair and tumor suppression...
  6. Kim Y, Lach F, Desetty R, Hanenberg H, Auerbach A, Smogorzewska A. Mutations of the SLX4 gene in Fanconi anemia. Nat Genet. 2011;43:142-6 pubmed publisher
  7. Fekairi S, Scaglione S, Chahwan C, Taylor E, Tissier A, Coulon S, et al. Human SLX4 is a Holliday junction resolvase subunit that binds multiple DNA repair/recombination endonucleases. Cell. 2009;138:78-89 pubmed publisher
    ..Furthermore, we show that SLX4 binds the XPF(ERCC4) and MUS81 subunits of the XPF-ERCC1 and MUS81-EME1 endonucleases and is required for DNA interstrand crosslink repair...
  8. Mazina O, Mazin A. Human Rad54 protein stimulates human Mus81-Eme1 endonuclease. Proc Natl Acad Sci U S A. 2008;105:18249-54 pubmed publisher
    Rad54, a key protein of homologous recombination, physically interacts with a DNA structure-specific endonuclease, Mus81-Eme1. Genetic data indicate that Mus81-Eme1 and Rad54 might function together in the repair of damaged DNA...
  9. Franchitto A, Pirzio L, Prosperi E, Sapora O, Bignami M, Pichierri P. Replication fork stalling in WRN-deficient cells is overcome by prompt activation of a MUS81-dependent pathway. J Cell Biol. 2008;183:241-52 pubmed publisher
    ..Knockdown of the MUS81 endonuclease in WRN-deficient cells completely prevents the accumulation of DSBs after fork stalling...

More Information


  1. Boddy M, Lopez Girona A, Shanahan P, Interthal H, Heyer W, Russell P. Damage tolerance protein Mus81 associates with the FHA1 domain of checkpoint kinase Cds1. Mol Cell Biol. 2000;20:8758-66 pubmed
    ..Here we report that the forkhead-associated-1 (FHA1) protein-docking domain of Cds1 interacts with Mus81, an evolutionarily conserved damage tolerance protein...
  2. Ciccia A, Ling C, Coulthard R, Yan Z, Xue Y, Meetei A, et al. Identification of FAAP24, a Fanconi anemia core complex protein that interacts with FANCM. Mol Cell. 2007;25:331-43 pubmed
    ..Our data indicate that the FANCM/FAAP24 complex may play a key role in recruitment of the FA core complex to damaged DNA. ..
  3. Ciccia A, Constantinou A, West S. Identification and characterization of the human mus81-eme1 endonuclease. J Biol Chem. 2003;278:25172-8 pubmed
    ..In fission yeast, the Mus81-Eme1 endonuclease complex (Mus81-Mms4 in Saccharomyces cerevisiae) has been implicated in the processing of ..
  4. Blais V, Gao H, Elwell C, Boddy M, Gaillard P, Russell P, et al. RNA interference inhibition of Mus81 reduces mitotic recombination in human cells. Mol Biol Cell. 2004;15:552-62 pubmed
    b>Mus81 is a highly conserved endonuclease with homology to the XPF subunit of the XPF-ERCC1 complex...
  5. Gao H, Chen X, McGowan C. Mus81 endonuclease localizes to nucleoli and to regions of DNA damage in human S-phase cells. Mol Biol Cell. 2003;14:4826-34 pubmed
    b>Mus81 is a highly conserved substrate specific endonuclease. Human Mus81 cleaves Holliday junctions, replication forks, and 3' flap substrates in vitro, suggesting a number of possible in vivo functions...
  6. Ciccia A, McDonald N, West S. Structural and functional relationships of the XPF/MUS81 family of proteins. Annu Rev Biochem. 2008;77:259-87 pubmed publisher
    Proteins belonging to the XPF/MUS81 family play important roles in the repair of DNA lesions caused by UV-light or DNA cross-linking agents...
  7. Taylor E, McGowan C. Cleavage mechanism of human Mus81-Eme1 acting on Holliday-junction structures. Proc Natl Acad Sci U S A. 2008;105:3757-62 pubmed publisher
    ..The human Mus81-Eme1 endonuclease is involved in recombination repair, but the exact structures it acts on in vivo are not known...
  8. Hanada K, Budzowska M, Davies S, van Drunen E, Onizawa H, Beverloo H, et al. The structure-specific endonuclease Mus81 contributes to replication restart by generating double-strand DNA breaks. Nat Struct Mol Biol. 2007;14:1096-104 pubmed
    ..Much less is known about the identities of these enzymes in mammalian cells. Mus81 is the catalytic component of a eukaryotic structure-specific endonuclease that preferentially cleaves branched ..
  9. Ying S, Minocherhomji S, Chan K, Palmai Pallag T, Chu W, Wass T, et al. MUS81 promotes common fragile site expression. Nat Cell Biol. 2013;15:1001-7 pubmed publisher
    ..Here, we report that the DNA structure-specific nuclease MUS81-EME1 localizes to CFS loci in early mitotic cells, and promotes the cytological appearance of characteristic gaps ..
  10. Zhang R, Sengupta S, Yang Q, Linke S, Yanaihara N, Bradsher J, et al. BLM helicase facilitates Mus81 endonuclease activity in human cells. Cancer Res. 2005;65:2526-31 pubmed
    ..Here, we report in vivo physical interaction and colocalization of BLM and a DNA structure-specific endonuclease, Mus81, at sites of stalled replication forks outside the promyelocytic leukemia nuclear bodies during the S-phase arrest ..
  11. Zeng S, Xiang T, Pandita T, Gonzalez Suarez I, Gonzalo S, Harris C, et al. Telomere recombination requires the MUS81 endonuclease. Nat Cell Biol. 2009;11:616-23 pubmed publisher
    ..Here we demonstrate that MUS81, a DNA structure specific recombination endonuclease, has a key role in the maintenance of telomeres in human ALT ..
  12. Shimura T, Torres M, Martin M, Rao V, Pommier Y, Katsura M, et al. Bloom's syndrome helicase and Mus81 are required to induce transient double-strand DNA breaks in response to DNA replication stress. J Mol Biol. 2008;375:1152-64 pubmed
    ..checkpoint, but they did activate a process that required functional Bloom's syndrome-associated (BLM) helicase, Mus81 nuclease and ataxia telangiectasia mutated and Rad3-related (ATR) kinase to induce transient double-stranded DNA ..
  13. Constantinou A, Chen X, McGowan C, West S. Holliday junction resolution in human cells: two junction endonucleases with distinct substrate specificities. EMBO J. 2002;21:5577-85 pubmed
    ..Like RuvC-mediated resolution, the products can be religated. The second, containing Mus81 protein, cuts Holliday junctions but the products are mostly non-ligatable...
  14. Wyatt H, Sarbajna S, Matos J, West S. Coordinated actions of SLX1-SLX4 and MUS81-EME1 for Holliday junction resolution in human cells. Mol Cell. 2013;52:234-47 pubmed publisher
    ..Here, we show that three structure-selective endonucleases, namely SLX1-SLX4, MUS81-EME1, and GEN1, define two pathways of HJ resolution in human cells...
  15. Fadden A, Schalbetter S, Bowles M, Harris R, Lally J, Carr A, et al. A winged helix domain in human MUS81 binds DNA and modulates the endonuclease activity of MUS81 complexes. Nucleic Acids Res. 2013;41:9741-52 pubmed publisher
    The MUS81-EME1 endonuclease maintains metazoan genomic integrity by cleaving branched DNA structures that arise during the resolution of recombination intermediates. In humans, MUS81 also forms a poorly characterized complex with EME2...
  16. Shin Y, Amangyeld T, Nguyen T, Munashingha P, Seo Y. Human MUS81 complexes stimulate flap endonuclease 1. FEBS J. 2012;279:2412-30 pubmed publisher
    The yeast heterodimeric Mus81-Mms4 complex possesses a structure-specific endonuclease activity that is critical for the restart of stalled replication forks and removal of toxic recombination intermediates...
  17. Regairaz M, Zhang Y, Fu H, Agama K, Tata N, Agrawal S, et al. Mus81-mediated DNA cleavage resolves replication forks stalled by topoisomerase I-DNA complexes. J Cell Biol. 2011;195:739-49 pubmed publisher
    ..We show that the structure-specific endonuclease Mus81-Eme1 is responsible for generating DSBs in response to Top1 inhibition and for allowing cell survival...
  18. Forment J, Blasius M, Guerini I, Jackson S. Structure-specific DNA endonuclease Mus81/Eme1 generates DNA damage caused by Chk1 inactivation. PLoS ONE. 2011;6:e23517 pubmed publisher
    ..double-strand breaks at replication forks when Chk1 activity is compromised relies on the DNA endonuclease complex Mus81/Eme1...
  19. Matos J, Blanco M, Maslen S, Skehel J, West S. Regulatory control of the resolution of DNA recombination intermediates during meiosis and mitosis. Cell. 2011;147:158-72 pubmed publisher
    ..In yeast meiosis, Mus81-Mms4 and Yen1 are controlled by phosphorylation events that lead to their sequential activation...
  20. Hiyama T, Katsura M, Yoshihara T, Ishida M, Kinomura A, Tonda T, et al. Haploinsufficiency of the Mus81-Eme1 endonuclease activates the intra-S-phase and G2/M checkpoints and promotes rereplication in human cells. Nucleic Acids Res. 2006;34:880-92 pubmed
    The Mus81-Eme1 complex is a structure-specific endonuclease that preferentially cleaves nicked Holliday junctions, 3'-flap structures and aberrant replication fork structures...
  21. Chang J, Kim J, Choi J, Lee J, Cho Y. Crystal structure of the Mus81-Eme1 complex. Genes Dev. 2008;22:1093-106 pubmed publisher
    The Mus81-Eme1 complex is a structure-specific endonuclease that plays an important role in rescuing stalled replication forks and resolving the meiotic recombination intermediates in eukaryotes...
  22. Wu F, Shirahata A, Sakuraba K, Kitamura Y, Goto T, Saito M, et al. Down-regulation of Mus81 as a potential marker for the malignancy of gastric cancer. Anticancer Res. 2010;30:5011-4 pubmed
    The Mus81 gene encodes a critical endonuclease involved in DNA repair and tumor suppression. In the present study, the role of Mus81 in gastric cancer was explored...
  23. Garner E, Kim Y, Lach F, Kottemann M, Smogorzewska A. Human GEN1 and the SLX4-associated nucleases MUS81 and SLX1 are essential for the resolution of replication-induced Holliday junctions. Cell Rep. 2013;5:207-15 pubmed publisher
    ..In vitro, HJs may be nucleolytically processed by MUS81-EME1, GEN1, and SLX4-SLX1...
  24. Elango R, Sheng Z, Jackson J, DeCata J, Ibrahim Y, Pham N, et al. Break-induced replication promotes formation of lethal joint molecules dissolved by Srs2. Nat Commun. 2017;8:1790 pubmed publisher
    ..Rare survivors in the absence of Srs2 rely on structure-specific endonucleases, Mus81 and Yen1, that resolve toxic joint-molecules...
  25. Amangyeld T, Shin Y, Lee M, Kwon B, Seo Y. Human MUS81-EME2 can cleave a variety of DNA structures including intact Holliday junction and nicked duplex. Nucleic Acids Res. 2014;42:5846-62 pubmed publisher
    b>MUS81 shares a high-degree homology with the catalytic XPF subunit of the XPF-ERCC1 endonuclease complex...
  26. Yin J, Wan B, Sarkar J, Horvath K, Wu J, Chen Y, et al. Dimerization of SLX4 contributes to functioning of the SLX4-nuclease complex. Nucleic Acids Res. 2016;44:4871-80 pubmed publisher
    ..Fanconi anemia protein SLX4 assembles a genome and telomere maintenance toolkit, consisting of the nucleases SLX1, MUS81 and XPF...
  27. Kurzbauer M, Pradillo M, Kerzendorfer C, Sims J, Ladurner R, Oliver C, et al. Arabidopsis thaliana FANCD2 Promotes Meiotic Crossover Formation. Plant Cell. 2018;30:415-428 pubmed publisher
    ..i>AtFANCD2 promotes noninterfering COs in a MUS81-independent manner and is therefore part of an uncharted meiotic CO-promoting mechanism, in addition to those ..
  28. Tian Y, Paramasivam M, Ghosal G, Chen D, Shen X, Huang Y, et al. UHRF1 contributes to DNA damage repair as a lesion recognition factor and nuclease scaffold. Cell Rep. 2015;10:1957-66 pubmed publisher
    ..UHRF1 interacts physically with both ERCC1 and MUS81, two nucleases involved in the repair of ICL lesions...
  29. Mayle R, Campbell I, Beck C, Yu Y, Wilson M, Shaw C, et al. DNA REPAIR. Mus81 and converging forks limit the mutagenicity of replication fork breakage. Science. 2015;349:742-7 pubmed publisher
    ..Pol32-dependent synthesis, but that mutagenic synthesis is limited to within a few kilobases from the break by Mus81 endonuclease and a converging fork...
  30. Colosio A, Frattini C, Pellicanò G, Villa Hernández S, Bermejo R. Nucleolytic processing of aberrant replication intermediates by an Exo1-Dna2-Sae2 axis counteracts fork collapse-driven chromosome instability. Nucleic Acids Res. 2016;44:10676-10690 pubmed
    ..Genetic analyses demonstrated a functional interplay of Exo1 with Mus81, Dna2 and Sae2 nucleases in promoting cell survival following replication stress, suggestive of concerted ..
  31. Ölmezer G, Levikova M, Klein D, Falquet B, Fontana G, Cejka P, et al. Replication intermediates that escape Dna2 activity are processed by Holliday junction resolvase Yen1. Nat Commun. 2016;7:13157 pubmed publisher
    ..Yen1, but not the HJ resolvases Slx1-Slx4 and Mus81-Mms4, safeguards chromosome segregation by removing replication intermediates that escape Dna2...
  32. Wyatt H, Laister R, Martin S, Arrowsmith C, West S. The SMX DNA Repair Tri-nuclease. Mol Cell. 2017;65:848-860.e11 pubmed publisher
    ..Resolution of these potentially toxic structures requires the MUS81-EME1 endonuclease, which is activated at prometaphase by formation of the SMX tri-nuclease containing three DNA ..
  33. Klemm T, Mannuß A, Kobbe D, Knoll A, Trapp O, Dorn A, et al. The DNA translocase RAD5A acts independently of the other main DNA repair pathways, and requires both its ATPase and RING domain for activity in Arabidopsis thaliana. Plant J. 2017;91:725-740 pubmed publisher
    ..of two main pathways of replication-associated DNA repair defined by the helicase RECQ4A and the endonuclease MUS81. The enhanced sensitivity of all double mutants tested in this study indicates that the repair of damaged DNA by ..
  34. Piazza A, Wright W, Heyer W. Multi-invasions Are Recombination Byproducts that Induce Chromosomal Rearrangements. Cell. 2017;170:760-773.e15 pubmed publisher
    ..spatial proximity of the donors and depends on the overlapping activities of the structure-selective endonucleases Mus81-Mms4, Slx1-Slx4, and Yen1...
  35. Stringer J, Counter C. Snm1B interacts with PSF2. PLoS ONE. 2012;7:e49626 pubmed publisher
    ..Ectopic expression of PSF2 increased the amount of Mus81, a protein component of the endonucleolytic complex involved in ICL repair, co-immunoprecipitating with Snm1B...
  36. Nomura Y, Adachi N, Koyama H. Human Mus81 and FANCB independently contribute to repair of DNA damage during replication. Genes Cells. 2007;12:1111-22 pubmed
    ..In mammals, the Mus81 endonuclease and the Fanconi anemia (FA) pathway have been implicated in HR repair; however, their functional ..
  37. Lemaçon D, Jackson J, Quinet A, Brickner J, Li S, Yazinski S, et al. MRE11 and EXO1 nucleases degrade reversed forks and elicit MUS81-dependent fork rescue in BRCA2-deficient cells. Nat Commun. 2017;8:860 pubmed publisher
    ..Next, we show that the initial limited resection of the regressed arms establishes the substrate for MUS81 in BRCA2-deficient cells...
  38. Palma A, Pugliese G, Murfuni I, Marabitti V, Malacaria E, Rinalducci S, et al. Phosphorylation by CK2 regulates MUS81/EME1 in mitosis and after replication stress. Nucleic Acids Res. 2018;46:5109-5124 pubmed publisher
    The MUS81 complex is crucial for preserving genome stability through the resolution of branched DNA intermediates in mitosis. However, untimely activation of the MUS81 complex in S-phase is dangerous...
  39. Schuster B, Knies K, Stoepker C, Velleuer E, Friedl R, Gottwald Mühlhauser B, et al. Whole exome sequencing reveals uncommon mutations in the recently identified Fanconi anemia gene SLX4/FANCP. Hum Mutat. 2013;34:93-6 pubmed publisher
    ..detected no residual SLX4 protein, which was consistent with abrogated interactions with XPF/ERCC1 and MUS81/EME1...
  40. Keyamura K, Arai K, Hishida T. Srs2 and Mus81-Mms4 Prevent Accumulation of Toxic Inter-Homolog Recombination Intermediates. PLoS Genet. 2016;12:e1006136 pubmed publisher
    ..The structure-specific complex of crossover junction endonucleases Mus81 and Mms4 was also required for viability of diploid, but not haploid, SRS2 deletion mutants (srs2?), and diploid ..
  41. Wan B, Yin J, Horvath K, Sarkar J, Chen Y, Wu J, et al. SLX4 assembles a telomere maintenance toolkit by bridging multiple endonucleases with telomeres. Cell Rep. 2013;4:861-9 pubmed publisher
    ..complex with the TRFH domain of TRF2 (TRF2TRFH) and map the interactions of SLX4 with endonucleases SLX1, XPF, and MUS81. TRF2 recognizes a unique HxLxP motif on SLX4 via the peptide-binding site in its TRFH domain...
  42. Berger G, Lawrence M, Hué S, Neil S. G2/M cell cycle arrest correlates with primate lentiviral Vpr interaction with the SLX4 complex. J Virol. 2015;89:230-40 pubmed publisher
    ..human immunodeficiency virus type 1 (HIV-1) viral protein R (Vpr) mediates this through activation of the SLX4/MUS81/EME1 exonuclease complex that forms part of the Fanconi anemia DNA repair pathway...
  43. Olivier M, Da Ines O, Amiard S, Serra H, Goubely C, White C, et al. The Structure-Specific Endonucleases MUS81 and SEND1 Are Essential for Telomere Stability in Arabidopsis. Plant Cell. 2016;28:74-86 pubmed publisher
    ..However, combined absence of the endonucleases MUS81 and SEND1 results in severe developmental defects, spontaneous cell death, and genome instability...
  44. Kim J, Harris S, Dinter T, Shah K, Mirkin S. The role of break-induced replication in large-scale expansions of (CAG)n/(CTG)n repeats. Nat Struct Mol Biol. 2017;24:55-60 pubmed publisher
    ..Genetic analysis revealed that Rad51, Rad52, Mre11, Pol32, Pif1, and Mus81 and/or Yen1 proteins are required for large-scale expansions, whereas proteins previously implicated in small-..
  45. Minocherhomji S, Ying S, Bjerregaard V, Bursomanno S, Aleliunaite A, Wu W, et al. Replication stress activates DNA repair synthesis in mitosis. Nature. 2015;528:286-90 pubmed publisher
    ..The MUS81-EME1 structure-specific endonuclease promotes the appearance of chromosome gaps or breaks at CFSs following ..
  46. Brégnard C, Benkirane M, Laguette N. DNA damage repair machinery and HIV escape from innate immune sensing. Front Microbiol. 2014;5:176 pubmed publisher
    ..Within this review we discuss the impact of this finding on our understanding of the interplay between HIV replication and nucleic acid metabolism and its implications for cancer-related chronic inflammation. ..
  47. Bogliolo M, Schuster B, Stoepker C, Derkunt B, Su Y, Raams A, et al. Mutations in ERCC4, encoding the DNA-repair endonuclease XPF, cause Fanconi anemia. Am J Hum Genet. 2013;92:800-6 pubmed publisher
  48. DePaula Silva A, Cassiday P, Chumley J, Bosque A, Monteiro Filho C, Mahon C, et al. Determinants for degradation of SAMHD1, Mus81 and induction of G2 arrest in HIV-1 Vpr and SIVagm Vpr. Virology. 2015;477:10-17 pubmed publisher
    ..We tested these constructs for their abilities to induce G2 arrest in human cells and to degrade agmSAMHD1 and Mus81. We found that the C-terminus of HIV-1 Vpr, when transferred onto SIVagm Vpr, provides the latter with the de novo ..
  49. Ghosal G, Leung J, Nair B, Fong K, Chen J. Proliferating cell nuclear antigen (PCNA)-binding protein C1orf124 is a regulator of translesion synthesis. J Biol Chem. 2012;287:34225-33 pubmed publisher
    ..Thus, C1orf124 acts at multiple steps in TLS, stabilizes RAD18 and ubiquitinated PCNA at damage sites, and facilitates the switch from replicative to TLS polymerase to bypass DNA lesion. ..
  50. Bhowmick R, Minocherhomji S, Hickson I. RAD52 Facilitates Mitotic DNA Synthesis Following Replication Stress. Mol Cell. 2016;64:1117-1126 pubmed publisher
    ..This mitotic DNA synthesis, termed MiDAS, requires the MUS81-EME1 endonuclease and a non-catalytic subunit of the Pol-delta complex, POLD3...
  51. Fu H, Martin M, Regairaz M, Huang L, You Y, Lin C, et al. The DNA repair endonuclease Mus81 facilitates fast DNA replication in the absence of exogenous damage. Nat Commun. 2015;6:6746 pubmed publisher
    The Mus81 endonuclease resolves recombination intermediates and mediates cellular responses to exogenous replicative stress...
  52. Dehé P, Gaillard P. Control of structure-specific endonucleases to maintain genome stability. Nat Rev Mol Cell Biol. 2017;18:315-330 pubmed publisher
    ..including the xeroderma pigmentosum group F-complementing protein (XPF) and MMS and UV-sensitive protein 81 (MUS81)-dependent nucleases, and the flap endonuclease 1 (FEN1), XPG and XPG-like endonuclease 1 (GEN1) enzymes - during ..
  53. Pérez Oliva A, Lachaud C, Szyniarowski P, Muñoz I, MacArtney T, Hickson I, et al. USP45 deubiquitylase controls ERCC1-XPF endonuclease-mediated DNA damage responses. EMBO J. 2015;34:326-43 pubmed publisher
    ..Together, these results establish USP45 as a new regulator of XPF-ERCC1 crucial for efficient DNA repair. ..
  54. Duda H, Arter M, Gloggnitzer J, Teloni F, Wild P, Blanco M, et al. A Mechanism for Controlled Breakage of Under-replicated Chromosomes during Mitosis. Dev Cell. 2016;39:740-755 pubmed publisher loop between replication intermediates and an M-phase-specific structure-selective endonuclease, MUS81-SLX4, which renders DNA replication and mitosis mutually exclusive...
  55. Murfuni I, Basile G, Subramanyam S, Malacaria E, Bignami M, Spies M, et al. Survival of the replication checkpoint deficient cells requires MUS81-RAD52 function. PLoS Genet. 2013;9:e1003910 pubmed publisher
    ..cells, DNA double-strand breaks (DSBs) are produced during replication by the structure-specific endonuclease MUS81. The mechanism underlying MUS81-dependent cleavage, and the effect on chromosome integrity and viability of ..
  56. Chacón M, Delivani P, Tolić I. Meiotic Nuclear Oscillations Are Necessary to Avoid Excessive Chromosome Associations. Cell Rep. 2016;17:1632-1645 pubmed publisher
    ..Mis-segregation is rescued by overexpression of the Holliday junction resolvase Mus81, suggesting that prolonged pairing results in irresolvable recombination intermediates...
  57. Li C, Wang S, Yuan H, Li W, Li Z, Li X, et al. [The mutation and expression of MUS81 gene in laryngeal squamous cell carcinoma]. Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2008;25:560-5 pubmed
    To investigate the association of mutations and expression of MUS81 gene with the tumorigenesis and progression of laryngeal squamous cell carcinoma (LSCC)...
  58. Talhaoui I, Bernal M, Mazon G. The nucleolytic resolution of recombination intermediates in yeast mitotic cells. FEMS Yeast Res. 2016;16: pubmed publisher
    ..pombe) have proven effective in determining the major nucleases involved in the processing of such intermediates: Mus81-Mms4 and Yen1...
  59. Laguette N, Brégnard C, Hue P, Basbous J, Yatim A, Larroque M, et al. Premature activation of the SLX4 complex by Vpr promotes G2/M arrest and escape from innate immune sensing. Cell. 2014;156:134-45 pubmed publisher
    ..SLX4 induced the recruitment of VPRBP and kinase-active PLK1, enhancing the cleavage of DNA by SLX4-associated MUS81-EME1 endonucleases...
  60. Fugger K, Chu W, Haahr P, Kousholt A, Beck H, Payne M, et al. FBH1 co-operates with MUS81 in inducing DNA double-strand breaks and cell death following replication stress. Nat Commun. 2013;4:1423 pubmed publisher
    ..We also show that FBH1 through its helicase activity co-operates with the MUS81 nuclease in promoting the endonucleolytic DNA cleavage following prolonged replication stress...
  61. Ii M, Ii T, Brill S. Mus81 functions in the quality control of replication forks at the rDNA and is involved in the maintenance of rDNA repeat number in Saccharomyces cerevisiae. Mutat Res. 2007;625:1-19 pubmed
    Previous studies in yeast have suggested that the SGS1 DNA helicase or the Mus81-Mms4 structure-specific endonuclease is required to suppress the accumulation of lethal recombination intermediates during DNA replication...
  62. Wu F, Chen W, Yan L, Tan G, Li W, Zhu X, et al. Mus81 knockdown improves chemosensitivity of hepatocellular carcinoma cells by inducing S-phase arrest and promoting apoptosis through CHK1 pathway. Cancer Med. 2016;5:370-85 pubmed publisher
    As a critical endonuclease in DNA repair, Mus81 is traditionally regarded as a tumor suppressor, but recently correlated with the sensitivity of mitomycin C and 5-fluorouracil in colon cancer and breast cancer cells...
  63. Blanco M, Matos J. Hold your horSSEs: controlling structure-selective endonucleases MUS81 and Yen1/GEN1. Front Genet. 2015;6:253 pubmed publisher
    ..Recent studies on two conserved SSEs - MUS81 and Yen1/GEN1- uncovered multiple layers of regulation that operate to carefully tailor JM-processing according to ..
  64. Gwon G, Kim Y, Liu Y, Watson A, Jo A, Etheridge T, et al. Crystal structure of a Fanconi anemia-associated nuclease homolog bound to 5' flap DNA: basis of interstrand cross-link repair by FAN1. Genes Dev. 2014;28:2276-90 pubmed publisher
    ..The PaFAN1 structure provides insights into how archaeal Holliday junction resolvases evolved to incise 5' flap substrates and how FAN1 integrates with the FA complex to participate in ICL repair. ..
  65. Zhou X, Delucia M, Ahn J. SLX4-SLX1 Protein-independent Down-regulation of MUS81-EME1 Protein by HIV-1 Viral Protein R (Vpr). J Biol Chem. 2016;291:16936-16947 pubmed publisher
    Evolutionarily conserved structure-selective endonuclease MUS81 forms a complex with EME1 and further associates with another endonuclease SLX4-SLX1 to form a four-subunit complex of MUS81-EME1-SLX4-SLX1, coordinating distinctive ..
  66. Lin Y, Chahal H, Wu W, Cho H, Ransohoff K, Song F, et al. Association study of genetic variation in DNA repair pathway genes and risk of basal cell carcinoma. Int J Cancer. 2017;141:952-957 pubmed publisher
    ..93, P?=?1.35 × 10-6 ; rs659857 in exon of MUS81: OR?=?1.06, P?=?3.09 × 10-6 and rs57343616 in 3' UTR of NABP2: OR?=?1.11, P?=?6...
  67. Rondinelli B, Gogola E, Yücel H, Duarte A, van de Ven M, van der Sluijs R, et al. EZH2 promotes degradation of stalled replication forks by recruiting MUS81 through histone H3 trimethylation. Nat Cell Biol. 2017;19:1371-1378 pubmed publisher
    ..EZH2 localizes at stalled forks where it methylates Lys27 on histone 3 (H3K27me3), mediating recruitment of the MUS81 nuclease...
  68. Wyatt H, West S. Holliday junction resolvases. Cold Spring Harb Perspect Biol. 2014;6:a023192 pubmed publisher
    ..Finally, we review the biochemical and structural properties of some well-characterized resolvases from archaea, bacteriophage, and eukaryotes. ..
  69. Lilley D. Holliday junction-resolving enzymes-structures and mechanisms. FEBS Lett. 2017;591:1073-1082 pubmed publisher
    ..These complexes are dimerized within the crystal lattice such that the strands of the products may be simply reconnected to form a junction. These structures suggest a trajectory for the resolution process. ..
  70. Cohen E. From arrest to escape: HIV-1 Vpr cuts a deal. Cell Host Microbe. 2014;15:125-7 pubmed publisher
    ..In a recent issue of Cell, Laguette et al. (2014) demonstrate that untimely activation of the structure-specific endonuclease regulator SLX4 complex by Vpr promotes G2/M arrest and escape from innate immune sensing. ..
  71. Gwon G, Jo A, Baek K, Jin K, Fu Y, Lee J, et al. Crystal structures of the structure-selective nuclease Mus81-Eme1 bound to flap DNA substrates. EMBO J. 2014;33:1061-72 pubmed publisher
    The Mus81-Eme1 complex is a structure-selective endonuclease with a critical role in the resolution of recombination intermediates during DNA repair after interstrand cross-links, replication fork collapse, or double-strand breaks...
  72. Guenzel C, Hérate C, Benichou S. HIV-1 Vpr-a still "enigmatic multitasker". Front Microbiol. 2014;5:127 pubmed publisher
    ..In this review, we will summarize the main reported functions of HIV-1 Vpr and their significance in the context of the viral life cycle. ..
  73. González Prieto R, Cuijpers S, Luijsterburg M, van Attikum H, Vertegaal A. SUMOylation and PARylation cooperate to recruit and stabilize SLX4 at DNA damage sites. EMBO Rep. 2015;16:512-9 pubmed publisher
    ..Additionally, we elucidated a novel role for PARylation in the recruitment of SLX4 to sites of DNA damage. Combined, our results uncover how SLX4 is regulated by post-translational modifications. ..
  74. Pepe A, West S. MUS81-EME2 promotes replication fork restart. Cell Rep. 2014;7:1048-55 pubmed publisher
    ..Here, we show that the structure-selective endonuclease MUS81-EME2 is responsible for fork cleavage and restart in human cells...
  75. Saugar I, Jiménez Martín A, Tercero J. Subnuclear Relocalization of Structure-Specific Endonucleases in Response to DNA Damage. Cell Rep. 2017;20:1553-1562 pubmed publisher
    ..Here, we show that the structure-specific endonuclease Mus81-Mms4/EME1 relocalizes to subnuclear foci following DNA damage and colocalizes with the endonucleases Rad1-Rad10 (..