MGMT

Summary

Gene Symbol: MGMT
Description: O-6-methylguanine-DNA methyltransferase
Alias: 6-O-methylguanine-DNA methyltransferase, O-6-methylguanine-DNA-alkyltransferase, O6-methylguanine-DNA methyltransferase, methylated-DNA--protein-cysteine methyltransferase, methylguanine-DNA methyltransferase
Species: human

Top Publications

  1. ncbi MGMT promoter methylation status can predict the incidence and outcome of pseudoprogression after concomitant radiochemotherapy in newly diagnosed glioblastoma patients
    Alba A Brandes
    Department of Medical Oncology, Bellaria Maggiore Hospital, Azienda Unità Sanitaria Locale of Bologna, Bologna, Italy
    J Clin Oncol 26:2192-7. 2008
  2. ncbi The correlation and prognostic significance of MGMT promoter methylation and MGMT protein in glioblastomas
    Van Thang Cao
    Brain Tumor Research Laboratory and Research Institute of Medical Sciences, Chonnam National University, Hwasun Hospital and Medical School, Gwangju, Republic of Korea
    Neurosurgery 65:866-75; discussion 875. 2009
  3. ncbi Clinical trial substantiates the predictive value of O-6-methylguanine-DNA methyltransferase promoter methylation in glioblastoma patients treated with temozolomide
    Monika E Hegi
    Laboratory of Tumor Biology and Genetics of the Neurosurgery Departments of the University Hospitals, Lausanne, Switzerland
    Clin Cancer Res 10:1871-4. 2004
  4. ncbi Identification of regions correlating MGMT promoter methylation and gene expression in glioblastomas
    Sibille Everhard
    INSERM, U711, Batiment de la nouvelle Pharmacie, 47 bd de l Hopital, 75651 Paris Cedex 13, France
    Neuro Oncol 11:348-56. 2009
  5. ncbi O-methylguanine-DNA methyltransferase (MGMT) mRNA expression predicts outcome in malignant glioma independent of MGMT promoter methylation
    Simone Kreth
    Department of Anaesthesiology, Ludwig Maximilians University, Munich, Germany
    PLoS ONE 6:e17156. 2011
  6. ncbi MGMT promoter hypermethylation in a series of 104 glioblastomas
    Marta Mellai
    Neuro bio oncology Center of Policlinico di Monza Foundation, University of Turin, Via Pietro Micca, 29 13100 Vercelli, Italy
    Cancer Genomics Proteomics 6:219-27. 2009
  7. ncbi O(6)-methylguanine DNA-methyltransferase methylation status can change between first surgery for newly diagnosed glioblastoma and second surgery for recurrence: clinical implications
    Alba A Brandes
    Department of Medical Oncology, Azienda USL Bellaria Maggiore Hospital, Bologna, Italy
    Neuro Oncol 12:283-8. 2010
  8. ncbi MGMT activity, promoter methylation and immunohistochemistry of pretreatment and recurrent malignant gliomas: a comparative study on astrocytoma and glioblastoma
    Markus Christmann
    Institute of Toxicology, University Medical Center Mainz, D 55131 Mainz, Germany
    Int J Cancer 127:2106-18. 2010
  9. ncbi Prognostic significance of molecular markers and extent of resection in primary glioblastoma patients
    Jörg Felsberg
    Department of Neuropathology, Heinrich Heine University, Dusseldorf, Germany
    Clin Cancer Res 15:6683-93. 2009
  10. ncbi Promoter methylation analysis of O6-methylguanine-DNA methyltransferase in glioblastoma: detection by locked nucleic acid based quantitative PCR using an imprinted gene (SNURF) as a reference
    Luca Morandi
    Department of Haemathology and Oncological Sciences Section of Pathology, Bellaria Hospital, University of Bologna, Italy
    BMC Cancer 10:48. 2010

Research Grants

  1. Induction of MGMT as a Strategy for Chemoprevention
    Kalkunte Srivenugopal; Fiscal Year: 2007
  2. Hematopoietic Chemoprotection in Glioblastoma Patients
    Hans Peter Kiem; Fiscal Year: 2010
  3. Hematopoietic Chemoprotection in Glioblastoma Patients
    Hans Peter Kiem; Fiscal Year: 2007
  4. Epigenetic regulation of temozolomide responsiveness in glioblastoma
    Jann N Sarkaria; Fiscal Year: 2010
  5. Epigenetic regulation of temozolomide responsiveness in glioblastoma
    Jann Sarkaria; Fiscal Year: 2009
  6. Dual Targeting of DNA Repair and p53 pathways for treatment of brain cancer
    Karen E Pollok; Fiscal Year: 2010
  7. MODULATING BASE EXCISION REPAIR IN TUMOR DRUG RESISTANCE
    Stanton Gerson; Fiscal Year: 2007
  8. Effect of dietary components on DNA repair
    Michael Wyatt; Fiscal Year: 2004
  9. Biomarkers of survival in glioma epidemiology
    John K Wiencke; Fiscal Year: 2010
  10. Biomarkers of survival in glioma epidemiology
    John Wiencke; Fiscal Year: 2009

Detail Information

Publications213 found, 100 shown here

  1. ncbi MGMT promoter methylation status can predict the incidence and outcome of pseudoprogression after concomitant radiochemotherapy in newly diagnosed glioblastoma patients
    Alba A Brandes
    Department of Medical Oncology, Bellaria Maggiore Hospital, Azienda Unità Sanitaria Locale of Bologna, Bologna, Italy
    J Clin Oncol 26:2192-7. 2008
    ..The radiologic images obtained in this setting can be difficult to interpret since they may show radiation-induced pseudoprogression (psPD) rather than disease progression...
  2. ncbi The correlation and prognostic significance of MGMT promoter methylation and MGMT protein in glioblastomas
    Van Thang Cao
    Brain Tumor Research Laboratory and Research Institute of Medical Sciences, Chonnam National University, Hwasun Hospital and Medical School, Gwangju, Republic of Korea
    Neurosurgery 65:866-75; discussion 875. 2009
    The aim of this study was to evaluate the correlation and prognostic significance of MGMT promoter methylation and protein expression in patients with glioblastoma.
  3. ncbi Clinical trial substantiates the predictive value of O-6-methylguanine-DNA methyltransferase promoter methylation in glioblastoma patients treated with temozolomide
    Monika E Hegi
    Laboratory of Tumor Biology and Genetics of the Neurosurgery Departments of the University Hospitals, Lausanne, Switzerland
    Clin Cancer Res 10:1871-4. 2004
    ..trial, we determined the predictive value of the methylation status of the O-6-methylguanine-DNA methyltransferase (MGMT) promoter for outcome in glioblastoma patients treated with the alkylating agent temozolomide...
  4. ncbi Identification of regions correlating MGMT promoter methylation and gene expression in glioblastomas
    Sibille Everhard
    INSERM, U711, Batiment de la nouvelle Pharmacie, 47 bd de l Hopital, 75651 Paris Cedex 13, France
    Neuro Oncol 11:348-56. 2009
    The O(6)-methylguanine-DNA methyltransferase gene (MGMT) is methylated in several cancers, including gliomas...
  5. ncbi O-methylguanine-DNA methyltransferase (MGMT) mRNA expression predicts outcome in malignant glioma independent of MGMT promoter methylation
    Simone Kreth
    Department of Anaesthesiology, Ludwig Maximilians University, Munich, Germany
    PLoS ONE 6:e17156. 2011
    We analyzed prospectively whether MGMT (O(6)-methylguanine-DNA methyltransferase) mRNA expression gains prognostic/predictive impact independent of MGMT promoter methylation in malignant glioma patients undergoing radiotherapy with ..
  6. ncbi MGMT promoter hypermethylation in a series of 104 glioblastomas
    Marta Mellai
    Neuro bio oncology Center of Policlinico di Monza Foundation, University of Turin, Via Pietro Micca, 29 13100 Vercelli, Italy
    Cancer Genomics Proteomics 6:219-27. 2009
    To evaluate MGMT promoter hypermethylation as prognostic factor in a retrospective study of 104 cases of glioblastoma multiforme (GBM).
  7. ncbi O(6)-methylguanine DNA-methyltransferase methylation status can change between first surgery for newly diagnosed glioblastoma and second surgery for recurrence: clinical implications
    Alba A Brandes
    Department of Medical Oncology, Azienda USL Bellaria Maggiore Hospital, Bologna, Italy
    Neuro Oncol 12:283-8. 2010
    O(6)-methylguanine DNA-methyltransferase (MGMT) promoter methylation status is a prognostic factor in newly diagnosed glioblastoma patients. However, it is not yet clear whether, and if so how, MGMT methylation status may change...
  8. ncbi MGMT activity, promoter methylation and immunohistochemistry of pretreatment and recurrent malignant gliomas: a comparative study on astrocytoma and glioblastoma
    Markus Christmann
    Institute of Toxicology, University Medical Center Mainz, D 55131 Mainz, Germany
    Int J Cancer 127:2106-18. 2010
    The DNA repair protein O(6)-methylguanine-DNA methyltransferase (MGMT) is a key player in tumor cell resistance. Promoter methylation, MGMT activity and immunohistochemistry are used for determining the MGMT status...
  9. ncbi Prognostic significance of molecular markers and extent of resection in primary glioblastoma patients
    Jörg Felsberg
    Department of Neuropathology, Heinrich Heine University, Dusseldorf, Germany
    Clin Cancer Res 15:6683-93. 2009
    ..Recent data indicate that certain molecular markers, in particular MGMT promoter hypermethylation, are associated with response to alkylating chemotherapy and longer survival...
  10. ncbi Promoter methylation analysis of O6-methylguanine-DNA methyltransferase in glioblastoma: detection by locked nucleic acid based quantitative PCR using an imprinted gene (SNURF) as a reference
    Luca Morandi
    Department of Haemathology and Oncological Sciences Section of Pathology, Bellaria Hospital, University of Bologna, Italy
    BMC Cancer 10:48. 2010
    Epigenetic silencing of the MGMT gene by promoter methylation is associated with loss of MGMT expression, diminished DNA-repair activity and longer overall survival in patients with glioblastoma who, in addition to radiotherapy, received ..
  11. ncbi Prognostic value of MGMT promoter methylation in glioblastoma patients treated with temozolomide-based chemoradiation: a Portuguese multicentre study
    Bruno M Costa
    Life and Health Sciences Research Institute ICVS, Health Sciences School, University of Minho, Braga, Portugal
    Oncol Rep 23:1655-62. 2010
    ..The methylation status of the MGMT gene promoter is currently a promising molecular prognostic marker, but some controversial data have precluded its ..
  12. ncbi Promoter methylation and polymorphisms of the MGMT gene in glioblastomas: a population-based study
    Izabela Zawlik
    International Agency for Research on Cancer, Lyon, France
    Neuroepidemiology 32:21-9. 2009
    O(6)-methylguanine-DNA methyltransferase (MGMT) is a repair enzyme that removes promutagenic O(6)-methylguanine adducts in DNA, to protect cells from acquisition of G:C--> A:T mutations...
  13. ncbi MGMT methylation: a marker of response to temozolomide in low-grade gliomas
    Sibille Everhard
    Institut National de la Sante et de la Recherche Medicale, U711, Paris, France
    Ann Neurol 60:740-3. 2006
    ..0001). Assessment of MGMTP status could help identifying low-grade gliomas patients more likely to respond to chemotherapy or to benefit from MGMT depletion strategies.
  14. ncbi O6-methylguanine-DNA methyltransferase methylation and TP53 mutation in malignant astrocytomas and their relationships with clinical course
    Takao Watanabe
    Department of Neurological Surgery, Nihon University School of Medicine, 30 1 Oyaguchi kamumachi, Itabashi ku, Tokyo 173 8610, Japan
    Int J Cancer 113:581-7. 2005
    Epigenetic silencing of O(6)-methylguanine-DNA methyltransferase (MGMT) by promoter methylation can confer cancer cells with an increased sensitivity to alkylating chemotherapeutic agents and a higher susceptibility to TP53 transition ..
  15. ncbi Anti-O6-methylguanine-methyltransferase (MGMT) immunohistochemistry in glioblastoma multiforme: observer variability and lack of association with patient survival impede its use as clinical biomarker
    Matthias Preusser
    Department of Internal Medicine 1, Medial University of Vienna, Vienna, Austria
    Brain Pathol 18:520-32. 2008
    Silencing of O6-methylguanine-DNA methyltransferase (MGMT) protein expression because of MGMT gene promoter hypermethylation is considered to be associated with postoperative chemoradiotherapy benefits in glioblastoma multiforme (GBM) ..
  16. ncbi Intratumoral homogeneity of MGMT promoter hypermethylation as demonstrated in serial stereotactic specimens from anaplastic astrocytomas and glioblastomas
    Eva M Grasbon-Frodl
    Center for Neuropathology and Prion Research, Ludwig Maximilians Universitat Munchen, Munich, Germany
    Int J Cancer 121:2458-64. 2007
    Hypermethylation of the DNA repair gene O(6)-methyl-guanine DNA methyltransferase (MGMT) has been linked to prolonged survival in glioblastoma patients treated with alkylating agents...
  17. ncbi Pitfalls in the assessment of MGMT expression and in its correlation with survival in diffuse astrocytomas: proposal of a feasible immunohistochemical approach
    David Capper
    Institute of Brain Research, University of Tubingen, Tubingen, Germany
    Acta Neuropathol 115:249-59. 2008
    Immunohistochemical studies showed that O(6)-methylguanine-DNA methyltransferase (MGMT) protein expression is negatively associated with survival in glioblastomas treated with alkylating agents in accordance with previous results of ..
  18. ncbi MGMT immunohistochemical expression and promoter methylation in human glioblastoma
    Fausto J Rodriguez
    Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN, USA
    Appl Immunohistochem Mol Morphol 16:59-65. 2008
    O6-methylguanine-DNA methyltransferase (MGMT) expression has been recently proposed as a useful prognostic and/or predictive marker in glioblastoma patients receiving adjuvant therapy after the surgery...
  19. ncbi Frequent promoter hypermethylation and low expression of the MGMT gene in oligodendroglial tumors
    Maria Möllemann
    Department of Neuropathology, Heinrich Heine University, Dusseldorf, Germany
    Int J Cancer 113:379-85. 2005
    ..The DNA repair enzyme O(6)-methylguanine DNA methyltransferase (MGMT) may cause resistance to DNA-alkylating drugs commonly used in the treatment of anaplastic ..
  20. ncbi The global DNA methylation surrogate LINE-1 methylation is correlated with MGMT promoter methylation and is a better prognostic factor for glioma
    Fumiharu Ohka
    Department of Neurosurgery, Nagoya University School of Medicine, Nagoya, Japan
    PLoS ONE 6:e23332. 2011
    ..Although GBM patients benefit from promoter hypermethylation of the O(6)-methylguanine-DNA methyltransferase (MGMT) that is the main determinant of resistance to TMZ, recent studies suggested that MGMT promoter methylation is of ..
  21. ncbi O(6)-Methylguanine DNA methyltransferase determined by promoter hypermethylation and immunohistochemical expression is correlated with progression-free survival in patients with glioblastoma
    Yukihiko Sonoda
    Department of Neurosurgery, Tohoku University Graduate School of Medicine, 1 1 Seiryo machi, Aoba ku, Sendai 980 8574, Japan
    Int J Clin Oncol 15:352-8. 2010
    The prognostic significance of O(6)-methylguanine DNA methyltransferase (MGMT) was evaluated by analysis of both MGMT promoter methylation and protein expression in a series of patients with newly diagnosed glioblastoma.
  22. ncbi Determination of the methylation status of MGMT in different regions within glioblastoma multiforme
    Mark G Hamilton
    Division of Neurosurgery, Department of Clinical Neurosciences, University of Calgary, Foothills Hospital 12th Floor 1403 29 ST NW 1195, Calgary, AB, T2N 2T9, Canada
    J Neurooncol 102:255-60. 2011
    Epigenetic silencing of the MGMT gene through promoter methylation correlates with improved survival in Glioblastoma Multiforme (GBM) patients receiving concurrent chemoradiotherapy...
  23. ncbi High incidence of MGMT promoter methylation in primary glioblastomas without correlation with TP53 gene mutations
    Emilia Jesien-Lewandowicz
    Department of Radiation Oncology, Chair of Oncology, Copernicus Memorial Hospital, Lodz, Poland
    Cancer Genet Cytogenet 188:77-82. 2009
    O(6)-methylguanine DNA methyltransferase (MGMT) reduces cytotoxicity of therapeutic or environmental alkylating agents...
  24. ncbi MGMT promoter methylation is predictive of response to radiotherapy and prognostic in the absence of adjuvant alkylating chemotherapy for glioblastoma
    Andreana L Rivera
    Departments of Pathology and Radiation Oncology, The University of Texas M D Anderson Cancer Center, 1515 Holcombe Boulevard, Unit 0097, Houston, TX 77030, USA
    Neuro Oncol 12:116-21. 2010
    Hypermethylation of the O(6)-methylguanine-DNA-methyltransferase (MGMT) gene has been shown to be associated with improved outcome in glioblastoma (GBM) and may be a predictive marker of sensitivity to alkylating agents...
  25. ncbi Promoter hypermethylation of multiple genes in early gastric adenocarcinoma and precancerous lesions
    Xiao Ping Zou
    Department of Gastroenterology, The Drum Tower Hospital Affiliated to Medical School of Nanjing University, Nanjing 210008, PR China
    Hum Pathol 40:1534-42. 2009
    ..In this study, we determined the methylation frequency of 5 genes, including p16, Runx3, MGMT, DAPK, and RASSF1A, by methylation-specific polymerase chain reaction, in a series of formalin-fixed paraffin-..
  26. ncbi Mutually exclusive promoter hypermethylation patterns of hMLH1 and O6-methylguanine DNA methyltransferase in colorectal cancer
    Edward J Fox
    Department of Pathology, Conway Institute of Biomolecular and Biomedical Research, University College Dublin, Belfield, Dublin 4, Ireland
    J Mol Diagn 8:68-75. 2006
    ..Transcriptional silencing of O6-methylguanine DNA methyltransferase (MGMT) has also been described in a variety of neoplasms and has been associated with a consequent ..
  27. ncbi DNA repair gene O6-methylguanine-DNA methyltransferase: promoter hypermethylation associated with decreased expression and G:C to A:T mutations of p53 in brain tumors
    Dong Yin
    Division of Hematology Oncology, Cedars Sinai Medical Center, UCLA School of Medicine, Los Angeles, California 90048, USA
    Mol Carcinog 36:23-31. 2003
    The DNA repair enzyme O(6)-methylguanine-DNA methyltransferase (MGMT) removes alkylating adducts from the O(6) position of guanine and protects cells from cytotoxic and mutagenic effects...
  28. ncbi Aberrant promoter hypermethylation of p16 and MGMT genes in oral squamous cell carcinomas and the surrounding normal mucosa
    Keizo Kato
    Department of Oral and Maxillofacial Sciences, Graduate School of Medicine, Gifu University, 1 1 Yanagido, Gifu, 501 1194, Japan
    J Cancer Res Clin Oncol 132:735-43. 2006
    ..Recent studies have shown roles of promoter hypermethylation of tumor suppressor genes, including p16 and MGMT, in several types of cancers...
  29. ncbi RASSF1A, BLU, NORE1A, PTEN and MGMT expression and promoter methylation in gliomas and glioma cell lines and evidence of deregulated expression of de novo DNMTs
    Aiala Lorente
    Brain Tumor Biology Unit, University of Navarra, Irunlarrea 1, Pamplona, Spain
    Brain Pathol 19:279-92. 2009
    ..1) and BLU (3p21.3) have been shown to be downregulated by methylation in cancer, and PTEN (10q23.3) and MGMT (10q26.1) are located in areas commonly deleted in astrocytomas...
  30. ncbi MGMT prognostic impact on glioblastoma is dependent on therapeutic modalities
    Emmanuelle Criniere
    INSERM, U711, Biologie des Interactions Neurones and Glie, Universite Pierre et Marie Curie, Faculte de Medecine, Paris, France
    J Neurooncol 83:173-9. 2007
    b>MGMT promoter methylation, which has been correlated with the response to alkylating agents, was investigated in a retrospective series of 219 glioblastomas (GBMs) treated with various modalities...
  31. ncbi O6-Methylguanine DNA methyltransferase protein expression in tumor cells predicts outcome of temozolomide therapy in glioblastoma patients
    Sabine Spiegl-Kreinecker
    Department of Neurosurgery, Wagner Jauregg Hospital, Wagner Jauregg Weg 15, 4020 Linz, Austria
    Neuro Oncol 12:28-36. 2010
    O(6)-Methylguanine DNA methyltransferase (MGMT) is implicated as a major predictive factor for treatment response to alkylating agents including temozolomide (TMZ) of glioblastoma multiforme (GBM) patients...
  32. ncbi Folate supplementation limits the aggressiveness of glioma via the remethylation of DNA repeats element and genes governing apoptosis and proliferation
    Eric Hervouet
    Institut National de la Sante et de la Recherche Medicale U892, Centre de Recherche en Cancerologie, Equipe Apoptose et progression tumorale, Nantes, France
    Clin Cancer Res 15:3519-29. 2009
    ....
  33. ncbi Careful exclusion of non-neoplastic brain components is required for an appropriate evaluation of O6-methylguanine-DNA methyltransferase status in glioma: relationship between immunohistochemistry and methylation analysis
    Ken Sasai
    Laboratory of Molecular and Cellular Pathology, Hokkaido University Graduate School of Medicine, Sapporo, Japan
    Am J Surg Pathol 32:1220-7. 2008
    Evaluation of O6-methylguanine-DNA methyltransferase (MGMT) expression is important for antiglioma therapy as many clinical trials have demonstrated that promoter hypermethylation and low level expression of MGMT are associated with an ..
  34. ncbi Serum DNA can define tumor-specific genetic and epigenetic markers in gliomas of various grades
    Iris Lavon
    Gaffin Center for Neuro Oncology and the Department of Neurology, Hadassah Hebrew University Medical Center, Ein Kerem, PO Box 12000, Jerusalem 91120, Israel
    Neuro Oncol 12:173-80. 2010
    ..The methylation status of O(6)-methyl guanine methyltransferase (MGMT) and phosphatase and tensin homolog promoters was studied by methylation-specific PCR...
  35. ncbi Silencing effect of CpG island hypermethylation and histone modifications on O6-methylguanine-DNA methyltransferase (MGMT) gene expression in human cancer
    Tetsuji Nakagawachi
    Division of Molecular Biology and Genetics, Department of Biomolecular Sciences, Saga Medical School, 5 1 1 Nabeshima, Saga 849 8501, Japan
    Oncogene 22:8835-44. 2003
    O6-methylguanine-DNA methyltransferase (MGMT) repairs the cytotoxic and mutagenic O6-alkylguanine produced by alkylating agents such as chemotherapeutic agents and mutagens...
  36. ncbi Association between MGMT promoter hypermethylation and p53 mutation in glioblastoma
    Jamal Shamsara
    Biotechnology Research Center, Mashhad, Iran
    Cancer Invest 27:825-9. 2009
    O(6)-methylguanine-DNA methyltransferase (MGMT) is a DNA repair enzyme that removes alkyl groups from the O(6) position of guanine. MGMT is transcriptionally silenced by promoter hypermethylation in several human neoplasia...
  37. ncbi Absence of MGMT promoter methylation in endometrial cancer
    B J Rimel
    Department of Obstetrics and Gynecology, Division of Gynecologic Oncology, Washington University School of Medicine, USA
    Gynecol Oncol 112:224-8. 2009
    O(6)-methylguanine-DNA methyltransferase (MGMT) acts to repair DNA damaged by alkylation of guanine residues...
  38. ncbi Hypermethylation of CpG island in O6-methylguanine-DNA methyltransferase gene was associated with K-ras G to A mutation in colorectal tumor
    Jian Qi
    Department of Gastroenterology, Zhongnan Hospital, Wuhan University, Wuhan 430071, Hubei Province, China
    World J Gastroenterol 11:2022-5. 2005
    To investigate the functions of promoter hypermethylation of O6-methylguanine-DNA methyltransferase (MGMT) gene in colorectal tumorigenesis and progression.
  39. ncbi Is inactivation of O6-methylguanine DNA methyltransferase still a favorable prognostic factor of patients with diffuse large B-cell lymphoma in the era of R-CHOP chemotherapy?
    Gyeong Won Lee
    Division of Hematology Oncology, Department of Internal Medicine, Gyeongsang National University School of Medicine, Jinju, Republic of Korea
    Leuk Lymphoma 50:1992-8. 2009
    The prognostic significance of O6-methylguanine DNA methyltransferase (MGMT) inactivation was evaluated in patients with diffuse large B-cell lymphoma (DLBCL) who received cyclophosphamide, doxorubicin, vincristine, and prednisone (CHOP) ..
  40. ncbi Detection of MGMT promoter methylation in normal individuals is strongly associated with the T allele of the rs16906252 MGMT promoter single nucleotide polymorphism
    Ida L M Candiloro
    Peter MaCallum Cancer Centre, St Andrew s Place, East Melbourne, Victoria 3002, Australia
    Cancer Prev Res (Phila) 2:862-7. 2009
    Methylation of the CpG island in the MGMT promoter region is a frequent event in several cancer types including colorectal cancer, lung cancer, lymphoma, and glioblastoma...
  41. ncbi Aberrant methylation of APC, MGMT, RASSF2A, and Wif-1 genes in plasma as a biomarker for early detection of colorectal cancer
    Bo Bin Lee
    Molecular Cell Biology, Samsung Medical Center, Seoul, Korea
    Clin Cancer Res 15:6185-91. 2009
    ..To identify epigenetic molecular makers in plasma for the early detection of colorectal cancer...
  42. ncbi Tumor progression through epigenetic gene silencing of O(6)-methylguanine-DNA methyltransferase in human biliary tract cancers
    Yasuo Koga
    Department of Surgery, Saga University Faculty of Medicine, Nabeshima 5 1 1, Saga 849 8501, Japan
    Ann Surg Oncol 12:354-63. 2005
    ..demonstrated in an immunohistochemical study that reduced expression of O(6)-methylguanine-DNA methyltransferase (MGMT) correlated with a poorer prognosis in patients with biliary tract cancers...
  43. ncbi O(6)-Methylguanine-DNA methyltransferase expression and prognostic value in brain metastases of lung cancers
    Pei Fang Wu
    Graduate Institute of Clinical Medicine, National Taiwan University, College of Medicine, Taipei, Taiwan
    Lung Cancer 68:484-90. 2010
    O(6)-Methylguanine-DNA methyltransferase (MGMT) is critical for repairing pro-mutagenic DNA bases and is correlated with response to alkylating agents in cancers...
  44. ncbi Alteration of O6-methylguanine-DNA methyltransferase in colorectal neoplasms in sporadic and familial adenomatous polyposis patients
    Sun Hee Kim
    Department of Pathology and Cancer Research Institute, Seoul National University College of Medicine, Seoul, Korea
    Mol Carcinog 37:32-8. 2003
    ..To investigate whether O(6)-methylguanine-DNA methyltransferase (MGMT) is altered during colorectal carcinogenesis, we performed immunohistochemical staining on 265 sporadic colorectal ..
  45. ncbi CpG methylation of MGMT and hMLH1 promoter in hepatocellular carcinoma associated with hepatitis viral infection
    S Matsukura
    Department of Biochemistry, Saga Medical School, Japan
    Br J Cancer 88:521-9. 2003
    Inactivations of DNA repair genes, O(6)-methylguanine-DNA methyltransferase (MGMT) and hMLH1, by promoter hypermethylation have been reported in several types of primary human neoplasia...
  46. ncbi Detailed methylation patterns and protein expression profiles of MGMT in colorectal carcinoma surgical margins
    Dingdong Zhang
    State Key Laboratory of Bioelectronics, Southeast University, Nanjing 210096, China
    Clin Biochem 41:19-25. 2008
    ..Hence, we examined MGMT expression profile and methylation status in histologically negative margins of colorectal cancer patients.
  47. ncbi Infrequent promoter methylation of the MGMT gene in liver metastases from uveal melanoma
    Verena Voelter
    Centre Pluridisciplinaire d Oncologie, Centre Hospitalier Universitaire Vaudois, University of Lausanne, Lausanne, Switzerland
    Int J Cancer 123:1215-8. 2008
    ..Here, we investigated whether the DNA-repair-protein MGMT may be involved in the variability of response to fotemustine and temozolomide in uveal melanoma...
  48. ncbi Prognostic significance of the immunohistochemical expression of O6-methylguanine-DNA methyltransferase, P-glycoprotein, and multidrug resistance protein-1 in glioblastomas
    Takao Nakagawa
    Department of Neurosurgery, Faculty of Medical Sciences, University of Fukui, Yoshida gun, Fukui, Japan
    Neuropathology 29:379-88. 2009
    We studied the expression of O(6)-methylguanine-DNA methyltransferase (O(6)-MGMT), P-glycoprotein (Pgp), and multidrug resistance protein-1 (MRP-1) in 23 glioblastomas using RT-PCR, methylation-specific PCR, and immunohistochemistry, and ..
  49. ncbi Silencing of MGMT expression by promoter hypermethylation in the metaplasia-dysplasia-carcinoma sequence of Barrett's esophagus
    Doerthe Kuester
    Department of Pathology, Otto von Guericke University Magdeburg, Germany
    Cancer Lett 275:117-26. 2009
    To determine the relevance of MGMT in Barrett's carcinogenesis, we analyzed promotor hypermethylation and expression of MGMT in Barrett's adenocarcinomas and its paired precursor lesions from 133 patients using a methylation-specific PCR, ..
  50. ncbi Silencing of O6-methylguanine DNA methyltransferase in the absence of promoter hypermethylation in hepatocellular carcinomas from Australia and South Africa
    Nirmitha I Herath
    Leukaemia Foundation of Queensland, Queensland Institute of Medical Research, Brisbane, QLD 4029, Australia
    Oncol Rep 17:817-22. 2007
    The DNA repair protein O6-methylguanine-DNA methyltransferase (MGMT) is involved in cellular defences against alkylating agents...
  51. ncbi O(6)-Methylguanine-DNA methyltransferase promoter hypermethylation shifts the p53 mutational spectrum in non-small cell lung cancer
    P Wolf
    Department of Surgery, Medical College of Wisconsin, 601 Elmwood Avenue, Milwaukee, WI 53226, USA
    Cancer Res 61:8113-7. 2001
    The DNA repair protein O6-methylguanine-DNA methyltransferase (MGMT) removes mutagenic adducts from the O6 position of guanine, thereby protecting the genome against G to A transition mutations...
  52. ncbi Low frequency of promoter methylation of O6-methylguanine DNA methyltransferase and hMLH1 in ulcerative colitis-associated tumors: comparison with sporadic colonic tumors
    Tetuo Mikami
    Department of Pathology, Kitasato University School of Medicine, Sagamihara, Japan
    Am J Clin Pathol 127:366-73. 2007
    ..to genesis of ulcerative colitis (UC)-associated tumors, promoter methylation and expression of O6-methylguanine DNA methyltransferase (MGMT), hMLH1, p16INK4, and E-cadherin were examined in 14 low-grade dysplasias (LGDs), 15 high-..
  53. ncbi Changes of the O6-methylguanine-DNA methyltransferase promoter methylation and MGMT protein expression after adjuvant treatment in glioblastoma
    Tae Young Jung
    Department of Neurosurgery, Chonnam National University Hwasun Hospital, Gwangju, Republic of Korea
    Oncol Rep 23:1269-76. 2010
    The aim of this study was to evaluate variations of O6-methylguanine-DNA methyltransferase (MGMT) promoter methylation and protein expression after adjuvant treatment in glioblastoma patients...
  54. ncbi Homogeneous MGMT immunoreactivity correlates with an unmethylated MGMT promoter status in brain metastases of various solid tumors
    Barbara Ingold
    Department of Pathology, Institute of Surgical Pathology, University Hospital Zurich, Zurich, Switzerland
    PLoS ONE 4:e4775. 2009
    The O(6)-methylguanine-methyltransferase (MGMT) promoter methylation status is a predictive parameter for the response of malignant gliomas to alkylating agents such as temozolomide...
  55. ncbi Inactivation of O6-methylguanine-DNA methyltransferase in soft tissue sarcomas: association with K-ras mutations
    Jeung Il Kim
    Department of Orthopedics, School of Medicine, Pusan National University, Busan 602 739, Republic of Korea
    Hum Pathol 40:934-41. 2009
    ..methyltransferase protein expression status using immunohistochemistry and promoter hypermethylation of the MGMT gene using methylation-specific PCR...
  56. ncbi Frequent promoter hypermethylation of the O6-Methylguanine-DNA Methyltransferase (MGMT) gene in testicular cancer
    B Smith-Sørensen
    Department of Tumour Biology, Institute for Cancer Research, The Norwegian Radium Hospital, 0310 Oslo, Norway
    Oncogene 21:8878-84. 2002
    ..A series of 70 tumours were analysed for methylation of CpG sites in the O(6)-methylguanine-DNA methyltransferase (MGMT) gene promoter, and in exon 1alpha of the cyclin-dependent kinase inhibitor 2A gene (CDKN2A)...
  57. ncbi MGMT promoter methylation is prognostic but not predictive for outcome to adjuvant PCV chemotherapy in anaplastic oligodendroglial tumors: a report from EORTC Brain Tumor Group Study 26951
    Martin J van den Bent
    Department of Neuro Oncology and Pathology, Daniel den Hoed Cancer Center and Erasmus University Medical Center, Rotterdam, The Netherlands
    J Clin Oncol 27:5881-6. 2009
    O6-methylguanine-methyltransferase (MGMT) promoter methylation has been shown to predict survival of patients with glioblastomas if temozolomide is added to radiotherapy (RT)...
  58. ncbi Variation of O(6)-methylguanine-DNA methyltransferase (MGMT) promoter methylation in serial samples in glioblastoma
    Jonathon F Parkinson
    Cancer Genetics Group, Kolling Institute of Medical Research, University of Sydney, St Leonards, NSW 2065, Australia
    J Neurooncol 87:71-8. 2008
    Methylation of the promoter region of the O ( 6 ) -methylguanine-DNA methyltransferase (MGMT) gene is known to be predictive of response to temozolomide treatment in patients with glioblastoma...
  59. ncbi The hypermethylation and protein expression of p16 INK4A and DNA repair gene O6-methylguanine-DNA methyltransferase in various uterine cervical lesions
    Zhenhua Lin
    Department of Pathology, Korea University College of Medicine, Seoul, South Korea
    J Cancer Res Clin Oncol 131:364-70. 2005
    This study is aimed at investigating the significance of gene promoter methylation status and protein expression of p16 INK4A and O6-methylguanine-DNA methyltransferase (MGMT) in the various uterine cervical lesions.
  60. ncbi O6-methylguanine-DNA methyltransferase is downregulated in transformed astrocyte cells: implications for anti-glioma therapies
    Ken Sasai
    Laboratory of Molecular and Cellular Pathology, Hokkaido University Graduate School of Medicine, Kita ku, Sapporo, Japan
    Mol Cancer 6:36. 2007
    ..However, expression of O6-methylguanine-DNA methyltransferase (MGMT) renders glioma cells resistant to the treatment, indicating that identification of mechanisms underlying the gene ..
  61. ncbi Validation of real-time methylation-specific PCR to determine O6-methylguanine-DNA methyltransferase gene promoter methylation in glioma
    Ilse Vlassenbroeck
    OncoMethylome Sciences SA, Tour 5 GIGA B34, 3, Avenue de l Hôpital 11, 4000 Liege, Belgium
    J Mol Diagn 10:332-7. 2008
    Epigenetic silencing of the DNA repair protein O(6)-methylguanine-DNA methyltransferase (MGMT) by promoter methylation predicts successful alkylating agent therapy, such as with temozolomide, in glioblastoma patients...
  62. ncbi Frequent intra-tumoural heterogeneity of promoter hypermethylation in malignant melanoma
    M Rastetter
    AWG Tumour Genetics of the Medical Faculty, Martin Luther University Halle Wittenberg, Germany
    Histol Histopathol 22:1005-15. 2007
    ..genes in melanoma, we analyzed the intra-tumoural distribution of promoter methylation of RASSF1A, p16, DAPK, MGMT, and Rb in 339 assays of 34 tumours (15 melanoma primaries, 19 metastases) by methylation-specific PCR, correlation ..
  63. ncbi Aberrant methylation and loss of expression of O-methylguanine-DNA methyltransferase in pulmonary squamous cell carcinoma and adenocarcinoma
    Osamu Furonaka
    Department of Pathology, Graduate School of Biomedical Sciences, Hiroshima University, Minami, Hiroshima, Japan
    Pathol Int 55:303-9. 2005
    O(6)-Methylguanine-DNA methyltransferase (MGMT) is a DNA repair protein that protects cells against the carcinogenic effects of alkylating agents...
  64. ncbi Aberrant DNA methylation of P16, MGMT, and hMLH1 genes in combination with MTHFR C677T genetic polymorphism in esophageal squamous cell carcinoma
    Jianming Wang
    Department of Epidemiology, School of Public Health, Fudan University, 138 Yi Xue Yuan Road, Shanghai, China
    Cancer Epidemiol Biomarkers Prev 17:118-25. 2008
    To explore the role of aberrant hypermethylation of cancer-related genes, such as P16, MGMT, and hMLH1, in the esophageal squamous cell carcinoma (ESCC) as well as its relation to dietary folate intake and MTHFR C677T polymorphism, we ..
  65. ncbi Methylation pattern of the O6-methylguanine-DNA methyltransferase gene in colon during progressive colorectal tumorigenesis
    Takeshi Nagasaka
    Department of Gastroenterological Surgery and Surgical Oncology, Okayama University Graduate School of Medicine and Dentistry, Okayama, Japan
    Int J Cancer 122:2429-36. 2008
    O(6)-methylguanine-DNA methyltransferase (MGMT) is a DNA repair gene which is frequently methylated in colorectal cancer (CRC)...
  66. ncbi Hypermethylation of O6-methylguanine-DNA methyltransferase promoter may predict nonrecurrence after chemotherapy in colorectal cancer cases
    Takeshi Nagasaka
    Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine and Dentistry, Okayama, Japan
    Clin Cancer Res 9:5306-12. 2003
    Because O(6)-methylguanine-DNA methyltransferase (MGMT) plays an essential role in repairing DNA damage caused by environmental alkylating chemicals, we were interested in determining whether we could see any obvious changes in the ..
  67. ncbi Promoter methylation of TIMP3 and CDH1 predicts better outcome in head and neck squamous cell carcinoma treated by radiotherapy only
    H De Schutter
    Leuvens Kanker Instituut, UH Leuven Campus Gasthuisberg, 3000 Leuven, Belgium
    Oncol Rep 21:507-13. 2009
    ..the incidence and prognostic value of the promoter hypermethylation of TIMP3, CDH1, DAPK, RASSF1A, p16INK4A and MGMT in HNSCC treated solely by radiotherapy...
  68. ncbi Ubiquitination-dependent proteolysis of O6-methylguanine-DNA methyltransferase in human and murine tumor cells following inactivation with O6-benzylguanine or 1,3-bis(2-chloroethyl)-1-nitrosourea
    K S Srivenugopal
    Department of Experimental Pediatrics, University of Texas M D Anderson Cancer Center, Houston 77030, USA
    Biochemistry 35:1328-34. 1996
    ..the role of ubiquitination in the disposition of the inactivated O6-methylguanine-DNA methyltransferase (MGMT) protein in human (HT-29 and CEM) and murine (ts85) tumor cells...
  69. ncbi Epigenetic regulation of O6-methylguanine-DNA methyltransferase gene expression by histone acetylation and methyl-CpG binding proteins
    Rebecca P Danam
    Department of Molecular Pharmacology, St Jude Children s Research Hospital 332 North Lauderdale Memphis, TN 38105, USA
    Mol Cancer Ther 4:61-9. 2005
    Transcriptional silencing of the DNA repair gene, O6-methylguanine-DNA methyltransferase (MGMT) in a proportion of transformed cell lines is associated with methylated CpG hotspots in the MGMT 5' flank...
  70. ncbi Folate deficiency alters hepatic and colon MGMT and OGG-1 DNA repair protein expression in rats but has no effect on genome-wide DNA methylation
    Susan J Duthie
    Rowett Institute of Nutrition and Health, University of Aberdeen, United Kingdom
    Cancer Prev Res (Phila) 3:92-100. 2010
    ..diet for 24 weeks on DNA damage (8-oxo-7,8-dihydroguanine), DNA repair [O(6)-methylguanine-DNA methyltransferase (MGMT) and 8-oxoguanine-DNA glycosylase (OGG-1) activity], and epigenetic parameters (genome-wide cytosine methylation ..
  71. ncbi Nomograms for predicting survival of patients with newly diagnosed glioblastoma: prognostic factor analysis of EORTC and NCIC trial 26981-22981/CE.3
    Thierry Gorlia
    EORTC Data Centre, Brussels, Belgium
    Lancet Oncol 9:29-38. 2008
    ....
  72. ncbi DNA polymorphism and risk of esophageal squamous cell carcinoma in a population of North Xinjiang, China
    Wen Jing Ma
    Medical Research Center, The First Affiliated Hospital, Xinjiang Medical University, Urumqi 830054, Xinjiang Uygur Autonomous Region, China
    World J Gastroenterol 16:641-7. 2010
    ..To investigate the role of metabolic enzyme and DNA repair genes in susceptibility of esophageal squamous cell carcinoma (ESCC)...
  73. ncbi O6-methylguanine-DNA methyltransferase, O6-benzylguanine, and resistance to clinical alkylators in pediatric primary brain tumor cell lines
    Michael S Bobola
    Division of Neurosurgery, Department of Surgery and Hematology Oncology, Children s Hospital and Regional Medical Center, Seattle, Washington 98105, USA
    Clin Cancer Res 11:2747-55. 2005
    ..Our purpose is (a) to assess the contribution of the DNA repair protein O6-methylguanine-DNA methyltransferase (MGMT) to the resistance of pediatric brain tumor cell lines to clinical alkylating agents and (b) to evaluate variables ..
  74. ncbi MGMT analysis at DNA, RNA and protein levels in glioblastoma tissue
    Matthias Preusser
    Department of Internal Medicine I, Medical University of Vienna, Waehringer Guertel 18 20, Vienna, Austria
    Histol Histopathol 24:511-8. 2009
    ..the epigenetic, transcriptional and translational regulation and expression of O6-methylguanine-methyltransferase (MGMT) is relevant for prognostic and predictive considerations in glioblastoma patients...
  75. ncbi Both base excision repair and O6-methylguanine-DNA methyltransferase protect against methylation-induced colon carcinogenesis
    Stefan Wirtz
    Department of Molecular Medicine, University of Erlangen Nuremberg, D 91052 Erlangen, German
    Carcinogenesis 31:2111-7. 2010
    ..The lesions are repaired by O(6)-methylguanine-DNA methyltransferase (Mgmt) and by enzymes of the base excision repair (BER) pathway, respectively...
  76. ncbi Predominant influence of MGMT methylation in non-resectable glioblastoma after radiotherapy plus temozolomide
    Niklas Thon
    Department of Neurosurgery, University Hospital Großhadern, Ludwig Maximilians Universitat Munchen, Munich, Germany
    J Neurol Neurosurg Psychiatry 82:441-6. 2011
    ..Unfortunately, no data are available concerning the predictive value of O(6)-methylguanine-DNA methyltransferase (MGMT) promoter methylation for this important subpopulation. For clarification, a prospective study was conducted.
  77. ncbi DNA repair in resistance to alkylating anticancer drugs
    B Kaina
    Institute of Toxicology, Division of Applied Toxicology, University of Mainz, Mainz, Gernany
    Int J Clin Pharmacol Ther 40:354-67. 2002
    ..DNA alkylation lesions can be repaired by the action of alkyltransferase (MGMT) and base excision repair enzymes...
  78. ncbi [Aberrant promoter methylation of tumor suppressor genes in serum from lung cancer patients: frequency and correlation with clinicopathological characteristics]
    Marius Zemaitis
    Department of Pulmonology and Immunology, Institute for Biomedical Research, Kaunas University of Medicine, Eiveniu 2, 50009 Kaunas, Lithuania
    Medicina (Kaunas) 41:123-31. 2005
    ....
  79. ncbi Modes of actions of two types of anti-neoplastic drugs, dacarbazine and ACNU, to induce apoptosis
    Masayuki Sanada
    Department of Physiological Science and Molecular Biology, Fukuoka Dental College, Fukuoka 814 0193, Japan
    Carcinogenesis 28:2657-63. 2007
    ..ACNU), respectively, can be repaired by O(6)-methylguanine-DNA methyltransferase (MGMT), coded by the MGMT gene...
  80. ncbi Aberrant promoter hypermethylation of multiple genes in head and neck squamous cell carcinoma
    Sajeev K Puri
    Department of Otolaryngology Head and Neck Surgery, University of Arkansas for Medical Sciences, Little Rock, Arkansas 72205, USA
    Am J Otolaryngol 26:12-7. 2005
    ..The aim of this study is to characterize and correlate, with clinical parameters, the promoter methylation profile of DNA repair genes, hMLH1 and O6-methylguanine-DNA methyltransferase (MGMT), and tumor-suppressor gene p16.
  81. ncbi A comparative study of the biochemical properties of human and mouse recombinant O6-methylguanine-DNA methyltransferases
    R Roy
    Sealy Center for Molecular Sciences, University of Texas Medical Branch, Galveston 77555
    Carcinogenesis 16:405-11. 1995
    The O6-methylguanine-DNA methyltransferase (MGMT) repairs mutagenic and carcinogenic O6-alkylguanine in DNA by accepting stoichiometrically the alkyl group from the base...
  82. ncbi Comprehensive analysis of MGMT promoter methylation: correlation with MGMT expression and clinical response in GBM
    Nameeta Shah
    The Ben and Catherine Ivy Center for Advanced Brain Tumor Treatment, Swedish Neuroscience Institute, Seattle, Washington, United States of America
    PLoS ONE 6:e16146. 2011
    O⁶-methylguanine DNA-methyltransferase (MGMT) promoter methylation has been identified as a potential prognostic marker for glioblastoma patients...
  83. ncbi Down-regulation of O6-methylguanine-DNA methyltransferase gene expression in gliomas by platinum compounds
    Satoshi Tanaka
    Department of Neurosurgery, The Kitasato Institute Medical Center Hospital, 6 100 Arai, Kitamoto, Saitama 364 8501, Japan
    Oncol Rep 14:1275-80. 2005
    ..0292 by Fisher's exact probability test). O(6)-methylguanine-DNA methyltransferase (MGMT) mRNA expression was measured twice by real-time quantitative reverse transcription-polymerase chain reaction (RT-..
  84. ncbi O6-methylguanine-DNA methyltransferase activity in cerebral gliomas. A guidance for nitrosourea treatment?
    K Mineura
    Neurological Service, Akita University Hospital, Japan
    Acta Oncol 33:29-32. 1994
    ..The activity of brain tissue, available from the peritumoral region of five cases, varied between 38 to 415 fmol/mg. Four of the five regions showed a higher value than the respective tumor, and one showed a lower value...
  85. ncbi Protection of hematopoietic cells from O(6)-alkylation damage by O(6)-methylguanine DNA methyltransferase gene transfer: studies with different O(6)-alkylating agents and retroviral backbones
    M Jansen
    Department of Internal Medicine Cancer Research, West German Cancer Center, University of Essen Medical School, Essen, Germany
    Eur J Haematol 67:2-13. 2001
    Overexpression of O(6)-methylguanine DNA methyltransferase (MGMT) can protect hematopoietic cells from O(6)-alkylation damage...
  86. ncbi Acquired resistance to 6-thioguanine in melanoma cells involves the repair enzyme O6-methylguanine-DNA methyltransferase (MGMT)
    Nir Gefen
    Dept of Microbiology and Immunology, Faculty of Health Sciences, Ben Gurion University, Beer Sheva, Israel
    Cancer Biol Ther 9:49-55. 2010
    O(6)-methylguanine-DNA methyltransferase (MGMT), is a DNA repair enzyme that recognizes O(6)-alkylated guanine, a base analog resulting from treatment with alkylating agents...
  87. ncbi Progressive low-grade oligodendrogliomas: response to temozolomide and correlation between genetic profile and O6-methylguanine DNA methyltransferase protein expression
    Netta Levin
    Leslie and Michael Gaffin Center for Neuro Oncology, Hadassah Hebrew University Hospital, Jerusalem, Israel imbar
    Cancer 106:1759-65. 2006
    ..The DNA repair enzyme O6-methylguanine DNA methyltransferase (MGMT) may induce resistance to DNA-alkylating agents...
  88. ncbi O6-methylguanine DNA methyltransferase status determined by promoter methylation and immunohistochemistry in gliosarcoma and their clinical implications
    Shin Hyuk Kang
    Department of Neurosurgery, Korea University Anam Hospital, Korea University College of Medicine, 126 1, 5 ga, Anam Dong, Seongbuk gu, Seoul, 136 705, Korea
    J Neurooncol 101:477-86. 2011
    O(6)-methylguanine-DNA methyltransferase (MGMT) is known as a DNA repair protein, and loss of function in MGMT is related to an increase in survival in patients with malignant gliomas treated with alkylating agents...
  89. ncbi Mice defective in the DNA mismatch gene PMS2 are hypersensitive to MNU induced thymic lymphoma and are partially protected by transgenic expression of human MGMT
    X Qin
    Division of Hematology Oncology, Case Western Reserve University, Cleveland, Ohio, OH 44106 4937, USA
    Oncogene 18:4394-400. 1999
    ..the sensitivity of PMS2 knockout mice to environmental carcinogens and the protective effect of O6-methylguanine DNA methyltransferase (MGMT), heterozygous PMS2 knockout mice and human MGMT (hMGMT) transgenic mice were mated and the ..
  90. ncbi Different repair of O6-methylguanine occurring in DNA modified by MMS in vivo or in vitro
    E Sledziewska-Gojska
    Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, Poland
    Mutat Res 383:31-7. 1997
    ..These mutations are avoided in adapted mutS- cells, when induced by MMS in vitro. This confirms that mismatch repair system of E. coli recognises mismatches formed in DNA by O6-methylguanine...
  91. ncbi Inhibition of O6-methylguanine-DNA methyltransferase by glucose-conjugated inhibitors: comparison with nonconjugated inhibitors and effect on fotemustine and temozolomide-induced cell death
    Bernd Kaina
    Institute of Toxicology, University of Mainz, Obere Zahlbacher Strasse 67, D 55131 Mainz, Germany
    J Pharmacol Exp Ther 311:585-93. 2004
    The DNA repair protein O(6)-methylguanine-DNA methyltransferase (MGMT) is an important suicide enzyme involved in the defense against O(6)-alkylating mutagens...
  92. ncbi Hyperthermostable protein structure maintained by intra and inter-helix ion-pairs in archaeal O6-methylguanine-DNA methyltransferase
    H Hashimoto
    Department of Materials Chemistry, Graduate School of Engineering, Osaka University, Suita, 565 0871, Japan
    J Mol Biol 292:707-16. 1999
    ..1.1.63) of hyperthermophilic archaeon Pyrococcuskodakaraensis strain KOD1 (Pk -MGMT) was determined by single isomorphous replacement method with anomalous scattering (SIRAS) at 1.8 A resolution...
  93. ncbi Temozolomide- and fotemustine-induced apoptosis in human malignant melanoma cells: response related to MGMT, MMR, DSBs, and p53
    S C Naumann
    Department of Toxicology, University of Mainz, Mainz, Germany
    Br J Cancer 100:322-33. 2009
    ..The O(6)-methylguanine-DNA methyltransferase (MGMT) activity in the cell lines was between 0 and 1100 fmol mg(-1) protein, and there was a correlation between MGMT ..
  94. ncbi Molecular correlates with MGMT promoter methylation and silencing support CpG island methylator phenotype-low (CIMP-low) in colorectal cancer
    Shuji Ogino
    Department of Medical Oncology, Dana Farber Cancer Institute, Harvard Medical School, 75 Francis St, BWH Pathology, Boston, MA 02115, USA
    Gut 56:1564-71. 2007
    ..O-6-methylguanine-DNA methyltransferase (MGMT) promoter methylation and silencing have been associated with G>A mutations and microsatellite instability-low (MSI-..
  95. ncbi The O6-methylguanine-DNA methyltransferase from the hyperthermophilic archaeon Pyrococcus sp. KOD1: a thermostable repair enzyme
    M M Leclere
    Department of Biotechnology, Graduate School of Engineering, Osaka University, Suita, Japan
    Mol Gen Genet 258:69-77. 1998
    The enzyme O6-methylguanine-DNA methyltransferase (MGMT) is the most common form of cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA...
  96. ncbi O6-methylguanine-DNA methyltransferase activity in adult gliomas: relation to patient and tumor characteristics
    J R Silber
    Department of Neurological Surgery, University of Washington, Seattle 98195 6470, USA
    Cancer Res 58:1068-73. 1998
    The DNA repair protein O6-methylguanine-DNA methyltransferase (MGMT) confers resistance to therapeutic methylating and chloroethylating agents in human brain tumor-derived cell lines...
  97. ncbi Co-expression of MGMT(P140K) and alpha-L-iduronidase in primary hepatocytes from mucopolysaccharidosis type I mice enables efficient selection with metabolic correction
    Daren Wang
    Cell and Molecular Therapy Program, and Division of Experimental Hematology, Cincinnati Children s Hospital Medical Center, Cincinnati, OH, USA
    J Gene Med 10:249-59. 2008
    ..It has been shown that an O(6)-methylguanine-DNA-methyltransferase variant (MGMT(P140K)) mediated in vivo selection of transduced hematopoietic stem cells (HSC) in animals.
  98. ncbi Individual adjuvant therapy for malignant gliomas based on O6-methylguanine-DNA methyltransferase messenger RNA quantitation by real-time reverse-transcription polymerase chain-reaction
    Satoshi Tanaka
    Department of Neurosurgery, Kawasaki Hospital, Ibaraki 313 8511, Japan
    Oncol Rep 20:165-71. 2008
    ..of real-time reverse-transcription polymerase chain-reaction (RT-PCR) for O6-methylguanine-DNA methyltransferase (MGMT), was used to treat malignant gliomas...
  99. ncbi Correlations between O6-methylguanine DNA methyltransferase promoter methylation status, 1p and 19q deletions, and response to temozolomide in anaplastic and recurrent oligodendroglioma: a prospective GICNO study
    Alba A Brandes
    Department of Medical Oncology, Bellaria Hospital, Via Altura 3, Bologna, Italy
    J Clin Oncol 24:4746-53. 2006
    ..The aim of this study was to evaluate correlations between 1p/19q deletions, O6-methylguanine DNA methyltransferase (MGMT) promoter methylation, and response rate to TMZ in this setting.
  100. ncbi Phase I pharmacokinetic and pharmacodynamic study of temozolomide in pediatric patients with refractory or recurrent leukemia: a Children's Oncology Group Study
    Terzah M Horton
    Texas Children s Cancer Center Baylor College of Medicine, Houston, TX 77030, USA
    J Clin Oncol 25:4922-8. 2007
    ..To determine the tolerability, pharmacokinetics, and mechanisms of temozolomide resistance in children with relapsed or refractory leukemia...
  101. ncbi O6-methylguanine-DNA methyltransferase-deficient phenotype in human gliomas: frequency and time to tumor progression after alkylating agent-based chemotherapy
    J R Silber
    Department of Neurological Surgery, University of Washington, Seattle 98195 6470, USA
    Clin Cancer Res 5:807-14. 1999
    The DNA repair protein O6-methylguanine-DNA methyltransferase (MGMT) contributes to the resistance of human brain tumor cell lines and xenografts to methylating and chloroethylating agents...

Research Grants75

  1. Induction of MGMT as a Strategy for Chemoprevention
    Kalkunte Srivenugopal; Fiscal Year: 2007
    ..b>MGMT (O6-methylguanine-DNA-methyltransferase), 6 which elicits a direct reversal of guanine-O6 alkylations, plays a ..
  2. Hematopoietic Chemoprotection in Glioblastoma Patients
    Hans Peter Kiem; Fiscal Year: 2010
    ..that genetic modification of hematopoietic stem cells with the P140K mutant of methylguanine methyltransferase (MGMT) results in chemoprotection and the ability to administer higher doses of temozolomide and BCNU when given in ..
  3. Hematopoietic Chemoprotection in Glioblastoma Patients
    Hans Peter Kiem; Fiscal Year: 2007
    ..that genetic modification of hematopoietic stem cells with the P140K mutant of methylguanine methyltransferase (MGMT) results in chemoprotection and the ability to administer higher doses of temozolomide and BCNU when given in ..
  4. Epigenetic regulation of temozolomide responsiveness in glioblastoma
    Jann N Sarkaria; Fiscal Year: 2010
    ..A key cytotoxic lesion induced by TMZ is repaired by the O6- methylguanine-methyltransferase (MGMT) DNA repair protein, and disruption of MGMT activity significantly increases the sensitivity of tumors to TMZ...
  5. Epigenetic regulation of temozolomide responsiveness in glioblastoma
    Jann Sarkaria; Fiscal Year: 2009
    ..A key cytotoxic lesion induced by TMZ is repaired by the O6- methylguanine-methyltransferase (MGMT) DNA repair protein, and disruption of MGMT activity significantly increases the sensitivity of tumors to TMZ...
  6. Dual Targeting of DNA Repair and p53 pathways for treatment of brain cancer
    Karen E Pollok; Fiscal Year: 2010
    ..primary cultures that differ in EGFR gene amplification, p53 status (wild-type or mutant), HDM2 status, MGMT expression, and sensitivities to TMZ and irradiation will be utilized...
  7. MODULATING BASE EXCISION REPAIR IN TUMOR DRUG RESISTANCE
    Stanton Gerson; Fiscal Year: 2007
    ..MMR) triggers apoptosis by aberrant repair but is successfully repaired by O6-methylguanine DNA-methyltransferase (MGMT)...
  8. Effect of dietary components on DNA repair
    Michael Wyatt; Fiscal Year: 2004
    ..pathways, including Base Excision Repair (BER), mismatch DNA repair (MMR), and methylguanine DNA-methyltransferase (MGMT), in the response to alkylating agent-induced DNA damage; and 2) the effect of dietary constituents on these DNA ..
  9. Biomarkers of survival in glioma epidemiology
    John K Wiencke; Fiscal Year: 2010
    ..In Aim 3 we will determine whether polymorphisms in MGMT or tumor TP53 mutation or expression influence survival in the presence or absence of tumor MGMT methylation in ..
  10. Biomarkers of survival in glioma epidemiology
    John Wiencke; Fiscal Year: 2009
    ..In Aim 3 we will determine whether polymorphisms in MGMT or tumor TP53 mutation or expression influence survival in the presence or absence of tumor MGMT methylation in ..
  11. DNA REPAIR DEFECTS AFFECTING HEMATOPOIETIC STEM CELLS
    Stanton Gerson; Fiscal Year: 2005
    ..methyl DNA adduct repair are mismatch repair [MMR] and 06-alkylguanine-DNA alkyltransferase [AGT], encoded by the MGMT gene...
  12. COMBATING ALKYLATING AGENT RESISTANCE IN HUMAN GLIOMAS
    JOHN SILBER; Fiscal Year: 2002
    ..agents is based on a mechanism(s) in addition to the DNA repair protein O6-methylguanine-DNA methyltransferase (MGMT)...
  13. ENZYMES AND REACTIONS FOR REPAIR OF DNA IN HUMAN CELLS
    THOMAS BRENT; Fiscal Year: 1993
    ..Such O6-alkylguanine- DNA alkyltransferase (MGMT previously called GATase) can incidentally repair precursors of cytotoxic DNA crosslinks induced by anticancer ..
  14. NEOPLASTIC COMPLICATIONS IN INFLAMMATORY BOWEL DISEASE
    Stephen Meltzer; Fiscal Year: 2007
    ..including E-cadherin (CDH 1), p 16, p 15, p 14-ARF, death-associated protein kinase (DAPK), O6-methylguanine DNA methyltransferase (MGMT), human mutL homolog 1 (hMLH 1), adenomatous polyposis coli (APC), RASSF1A, deleted in colon ..
  15. Treatment Study Using Depot Naltrexone(1/6)Philadelphia Coord/Data Mgmt Site
    CHARLES P O apos BRIEN; Fiscal Year: 2010
    ..The data from this study can also be used by the FDA to evaluate the benefits of this treatment that could improve the lives of patients suffering from opioid addiction. ..
  16. Neonatal Chemoselection Following Ex Vivo Gene Transfer For Hereditary Disorders
    KARIN L GAENSLER; Fiscal Year: 2010
    ..HSC will be transduced ex vivo by lentivirus-mediated gene transfer of P140K-methylguanine methytransferase (MGMT)...
  17. Development of Novel AIDS Gene Therapy Strategies in the Macaque SHIV Model
    Hans Peter Kiem; Fiscal Year: 2010
    ..The anti-HIV vectors we propose to test contain MGMT transgenes to allow in vivo selection post-transplantation...
  18. Development of Novel AIDS Gene Therapy Strategies in the Macaque SHIV Model
    Hans Peter Kiem; Fiscal Year: 2010
    ..The anti-HIV vectors we propose to test contain MGMT transgenes to allow in vivo selection post-transplantation...
  19. Role of DNA In Protecting Against Secondary Leukemias
    M Dolan; Fiscal Year: 2007
    ..but not addressed directly, is the possibility that the DNA repair protein, O6-alkylguanine-DNA alkyltransferase (MGMT), protects against toxicities associated with ifosfamide and cyclophosphamide...
  20. Gene Methylation and Therapeutic Response in Lung Cancer
    Steven Belinsky; Fiscal Year: 2005
    ..identified two excellent biomarkers, the p16 tumor suppressor gene and the O6-methylguanine-DNA methyltransferase (MGMT) repair gene, whose inactivations through aberrant CpG island promoter hypermethylation occur frequently and early ..
  21. TUMOR SUPPRESSOR GENE METHYLATION IN LUNG ADENOCARCINOMA
    Steven Belinsky; Fiscal Year: 2007
    ..Transient transfection of minimized promoters into cell lines with an endogenously active or silenced gene will be used to elucidate the sequence of changes involved in repression of promoter activity. REVISED: July 7, 2005 ..
  22. TUMOR SUPPRESSOR GENE METHYLATION IN LUNG ADENOCARCINOMA
    Steven Belinsky; Fiscal Year: 2009
    ..Transient transfection of minimized promoters into cell lines with an endogenously active or silenced gene will be used to elucidate the sequence of changes involved in repression of promoter activity. REVISED: July 7, 2005 ..
  23. TUMOR SUPPRESSOR GENE METHYLATION IN LUNG ADENOCARCINOMA
    Steven A Belinsky; Fiscal Year: 2010
    ..Transient transfection of minimized promoters into cell lines with an endogenously active or silenced gene will be used to elucidate the sequence of changes involved in repression of promoter activity. REVISED: July 7, 2005 ..