MED1

Summary

Gene Symbol: MED1
Description: mediator complex subunit 1
Alias: CRSP1, CRSP200, DRIP205, DRIP230, PBP, PPARBP, PPARGBP, RB18A, TRAP220, TRIP2, ARC205, PPAR binding protein, PPAR-binding protein, PPARG binding protein, TR-interacting protein 2, TRIP-2, activator-recruited cofactor 205 kDa component, mediator of RNA polymerase II transcription subunit 1, p53 regulatory protein RB18A, peroxisome proliferator-activated receptor-binding protein, thyroid hormone receptor-associated protein complex 220 kDa component, thyroid hormone receptor-associated protein complex component TRAP220, thyroid receptor interacting protein 2, thyroid receptor-interacting protein 2, vitamin D receptor-interacting protein 230 kD, vitamin D receptor-interacting protein complex component DRIP205
Species: human

Top Publications

  1. pmc Arginine and glutamate-rich 1 (ARGLU1) interacts with mediator subunit 1 (MED1) and is required for estrogen receptor-mediated gene transcription and breast cancer cell growth
    Dingxiao Zhang
    Department of Cancer and Cell Biology, College of Medicine, University of Cincinnati, Cincinnati, Ohio 45267, USA
    J Biol Chem 286:17746-54. 2011
  2. pmc The mediator complex functions as a coactivator for GATA-1 in erythropoiesis via subunit Med1/TRAP220
    Melanie Stumpf
    Department of Cellular and Molecular Immunology, Max Planck Institute of Immunobiology, Stubeweg 51, 79108 Freiburg, Germany
    Proc Natl Acad Sci U S A 103:18504-9. 2006
  3. pmc The TRAP/Mediator coactivator complex interacts directly with estrogen receptors alpha and beta through the TRAP220 subunit and directly enhances estrogen receptor function in vitro
    Yun Kyoung Kang
    Laboratory of Biochemistry and Molecular Biology, The Rockefeller University, New York, NY 10021, USA
    Proc Natl Acad Sci U S A 99:2642-7. 2002
  4. pmc Ligand-dependent interactions of coactivators steroid receptor coactivator-1 and peroxisome proliferator-activated receptor binding protein with nuclear hormone receptors can be imaged in live cells and are required for transcription
    J Llopis
    Department of Pharmacology, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093, USA
    Proc Natl Acad Sci U S A 97:4363-8. 2000
  5. pmc The DRIP complex and SRC-1/p160 coactivators share similar nuclear receptor binding determinants but constitute functionally distinct complexes
    C Rachez
    Cell Biology Program, Memorial Sloan Kettering Cancer Center, New York, NY 10021, USA
    Mol Cell Biol 20:2718-26. 2000
  6. pmc Mediator links epigenetic silencing of neuronal gene expression with x-linked mental retardation
    Ning Ding
    Department of Molecular Medicine, University of Texas Health Science Center at San Antonio, San Antonio, TX 78245, USA
    Mol Cell 31:347-59. 2008
  7. ncbi MED14 and MED1 differentially regulate target-specific gene activation by the glucocorticoid receptor
    Weiwei Chen
    Department of Microbiology, New York University Cancer Institute, New York University School of Medicine, 550 First Avenue, New York, New York 10016, USA
    Mol Endocrinol 20:560-72. 2006
  8. ncbi MED220/thyroid receptor-associated protein 220 functions as a transcriptional coactivator with Pit-1 and GATA-2 on the thyrotropin-beta promoter in thyrotropes
    David F Gordon
    Division of Endocrinology, Department of Medicine, University of Colorado Health Sciences Center Fitzsimons, Mail Stop 8106, P O Box 6511, Aurora, Colorado 80049, USA
    Mol Endocrinol 20:1073-89. 2006
  9. pmc Activation of TRAP/mediator subunit TRAP220/Med1 is regulated by mitogen-activated protein kinase-dependent phosphorylation
    Pradeep K Pandey
    Department of Physiology and Biophysics, Robert Wood Johnson Medical School, UMDNJ, Piscataway, NJ 08854, USA
    Mol Cell Biol 25:10695-710. 2005
  10. ncbi A novel human SRB/MED-containing cofactor complex, SMCC, involved in transcription regulation
    W Gu
    Laboratory of Biochemistry and Molecular Biology, Rockefeller University, New York, New York 10021, USA
    Mol Cell 3:97-108. 1999

Research Grants

Detail Information

Publications185 found, 100 shown here

  1. pmc Arginine and glutamate-rich 1 (ARGLU1) interacts with mediator subunit 1 (MED1) and is required for estrogen receptor-mediated gene transcription and breast cancer cell growth
    Dingxiao Zhang
    Department of Cancer and Cell Biology, College of Medicine, University of Cincinnati, Cincinnati, Ohio 45267, USA
    J Biol Chem 286:17746-54. 2011
    ..b>MED1 (Mediator subunit 1) is the key Mediator subunit that directly interacts with estrogen receptor to mediate its ..
  2. pmc The mediator complex functions as a coactivator for GATA-1 in erythropoiesis via subunit Med1/TRAP220
    Melanie Stumpf
    Department of Cellular and Molecular Immunology, Max Planck Institute of Immunobiology, Stubeweg 51, 79108 Freiburg, Germany
    Proc Natl Acad Sci U S A 103:18504-9. 2006
    ..Mediator subunit Med1/TRAP220 is a key component of Mediator originally found to associate with nuclear hormone receptors...
  3. pmc The TRAP/Mediator coactivator complex interacts directly with estrogen receptors alpha and beta through the TRAP220 subunit and directly enhances estrogen receptor function in vitro
    Yun Kyoung Kang
    Laboratory of Biochemistry and Molecular Biology, The Rockefeller University, New York, NY 10021, USA
    Proc Natl Acad Sci U S A 99:2642-7. 2002
    ..Studies with extracts from TRAP220(-/-) fibroblasts reveal that these interactions depend on TRAP220, a TRAP/Mediator subunit previously shown to ..
  4. pmc Ligand-dependent interactions of coactivators steroid receptor coactivator-1 and peroxisome proliferator-activated receptor binding protein with nuclear hormone receptors can be imaged in live cells and are required for transcription
    J Llopis
    Department of Pharmacology, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093, USA
    Proc Natl Acad Sci U S A 97:4363-8. 2000
    ..Here we demonstrate that both peroxisome proliferator-activated receptor binding protein (PBP) and steroid receptor coactivator-1 (SRC-1) are required for ligand-dependent transcription of transiently ..
  5. pmc The DRIP complex and SRC-1/p160 coactivators share similar nuclear receptor binding determinants but constitute functionally distinct complexes
    C Rachez
    Cell Biology Program, Memorial Sloan Kettering Cancer Center, New York, NY 10021, USA
    Mol Cell Biol 20:2718-26. 2000
    ..In this report, we characterize the nuclear receptor binding features of DRIP205, a key subunit of the DRIP complex, that interacts directly with VDR and thyroid hormone receptor in response to ..
  6. pmc Mediator links epigenetic silencing of neuronal gene expression with x-linked mental retardation
    Ning Ding
    Department of Molecular Medicine, University of Texas Health Science Center at San Antonio, San Antonio, TX 78245, USA
    Mol Cell 31:347-59. 2008
    ..These findings implicate Mediator in epigenetic restriction of neuronal gene expression to the nervous system and suggest a pathologic basis for MED12-associated XLMR involving impaired REST-dependent neuronal gene regulation...
  7. ncbi MED14 and MED1 differentially regulate target-specific gene activation by the glucocorticoid receptor
    Weiwei Chen
    Department of Microbiology, New York University Cancer Institute, New York University School of Medicine, 550 First Avenue, New York, New York 10016, USA
    Mol Endocrinol 20:560-72. 2006
    The Mediator subunits MED14 and MED1 have been implicated in transcriptional regulation by the glucocorticoid receptor (GR) by acting through its activation functions 1 and 2...
  8. ncbi MED220/thyroid receptor-associated protein 220 functions as a transcriptional coactivator with Pit-1 and GATA-2 on the thyrotropin-beta promoter in thyrotropes
    David F Gordon
    Division of Endocrinology, Department of Medicine, University of Colorado Health Sciences Center Fitzsimons, Mail Stop 8106, P O Box 6511, Aurora, Colorado 80049, USA
    Mol Endocrinol 20:1073-89. 2006
    ..Thus, the TSHbeta gene is maximally activated by a combination of three thyrotrope transcription factors that act via both protein-DNA and protein-protein interactions...
  9. pmc Activation of TRAP/mediator subunit TRAP220/Med1 is regulated by mitogen-activated protein kinase-dependent phosphorylation
    Pradeep K Pandey
    Department of Physiology and Biophysics, Robert Wood Johnson Medical School, UMDNJ, Piscataway, NJ 08854, USA
    Mol Cell Biol 25:10695-710. 2005
    ..b>TRAP220/Med1 is a key component of TRAP/Mediator that targets the complex to nuclear hormone receptors and other types of ..
  10. ncbi A novel human SRB/MED-containing cofactor complex, SMCC, involved in transcription regulation
    W Gu
    Laboratory of Biochemistry and Molecular Biology, Rockefeller University, New York, New York 10021, USA
    Mol Cell 3:97-108. 1999
    ..homologs of other yeast transcriptional regulatory factors (SOH1 and NUT2), and, significantly, some components (TRAP220, TRAP170/hRGR1, and TRAP100) of a human thyroid hormone receptor-associated coactivator complex...
  11. pmc Cooperative activity of cdk8 and GCN5L within Mediator directs tandem phosphoacetylation of histone H3
    Krista D Meyer
    Department of Chemistry and Biochemistry, Cellular and Developmental Biology, University of Colorado, Boulder, CO 80309, USA
    EMBO J 27:1447-57. 2008
    ..Indeed our results suggest that T/G-Mediator directs early events-such as modification of chromatin templates-in transcriptional activation...
  12. ncbi Spatio-temporal activation of chromatin on the human CYP24 gene promoter in the presence of 1alpha,25-Dihydroxyvitamin D3
    Sami Väisänen
    Department of Biochemistry, University of Kuopio, FIN 70211 Kuopio, Finland
    J Mol Biol 350:65-77. 2005
    ....
  13. ncbi Full activation of estrogen receptor alpha activation function-1 induces proliferation of breast cancer cells
    Tetsuo Fujita
    Institute of Applied Biochemistry, University of Tsukuba, 1 1 1 Tenno Dai, Tsukuba Science City, Ibaraki 305 8572, Japan
    J Biol Chem 278:26704-14. 2003
    ..These results indicated that fully activated AF-1 induces the stimulation of breast cancer growth and that the ratio between AF-1 coactivators and corepressors plays a key role to prevent proliferation of tumor by tamoxifen...
  14. ncbi Vitamin D-interacting protein 205 (DRIP205) coactivation of estrogen receptor alpha (ERalpha) involves multiple domains of both proteins
    Qian Wu
    Department of Biochemistry and Biophysics, Department of Veterinary Integrative Biosciences, Department of Veterinary Physiology and Pharmacology, Texas A and M University, College Station, TX 77843, USA
    J Biol Chem 279:53602-12. 2004
    Vitamin D-interacting protein 205 (DRIP205) is a mediator complex protein that anchors the complex to the estrogen receptor (ER) and other nuclear receptors (NRs)...
  15. pmc Regulation of the human cyclin C gene via multiple vitamin D3-responsive regions in its promoter
    Lasse Sinkkonen
    Department of Biochemistry, University of Kuopio PO Box 1627, FIN 70211 Kuopio, Finland
    Nucleic Acids Res 33:2440-51. 2005
    ....
  16. ncbi 2-Methylene-19-nor-(20S)-1,25-dihydroxyvitamin D3 potently stimulates gene-specific DNA binding of the vitamin D receptor in osteoblasts
    Hironori Yamamoto
    Department of Biochemistry, University of Wisconsin Madison, Madison, Wisconsin 53706, USA
    J Biol Chem 278:31756-65. 2003
    ..and ChIP indicate that 2MD is also more potent in promoting interaction with RXR and the coactivators SRC-1 and DRIP205. Finally, protease digestion studies revealed a unique VDR conformation in the presence of 2MD...
  17. ncbi Identity between TRAP and SMCC complexes indicates novel pathways for the function of nuclear receptors and diverse mammalian activators
    M Ito
    Laboratory of Biochemistry and Molecular Biology, Rockefeller University, New York, New York 10021, USA
    Mol Cell 3:361-70. 1999
    ..In parallel with ligand-dependent interactions of nuclear receptors with the TRAP220 subunit, p53 and VP16 activation domains interact directly with a newly cloned TRAP80 subunit...
  18. ncbi Transcription coactivator TRAP220 is required for PPAR gamma 2-stimulated adipogenesis
    Kai Ge
    Laboratory of Biochemistry and Molecular Biology, The Rockefeller University, 1230 York Avenue, New York, New York 10021, USA
    Nature 417:563-7. 2002
    ..This complex interacts physically with several nuclear receptors through the TRAP220 subunit, and with diverse activators through other subunits...
  19. ncbi Ligand-dependent transcription activation by nuclear receptors requires the DRIP complex
    C Rachez
    Cell Biology Program, Memorial Sloan Kettering Cancer Center, Graduate School of Medical Sciences, Cornell University, New York, New York 10021, USA
    Nature 398:824-8. 1999
    ..The role of nuclear-receptor ligands may, in part, be to recruit such a cofactor complex to the receptor and, in doing so, to enhance transcription of target genes...
  20. ncbi Isolation and characterization of PBP, a protein that interacts with peroxisome proliferator-activated receptor
    Y Zhu
    Department of Pathology, Northwestern University Medical School, Chicago, Illinois 60611, USA
    J Biol Chem 272:25500-6. 1997
    ..We now report the isolation of a cDNA encoding a 165-kDa PPARgamma-binding protein, designated PBP which also serves as a coactivator...
  21. ncbi Composite co-activator ARC mediates chromatin-directed transcriptional activation
    A M Näär
    Howard Hughes Medical Institute, Department of Molecular and Cell Biology, University of California, Berkeley 94720, USA
    Nature 398:828-32. 1999
    ..Thus, ARC/DRIP is a large composite co-activator that belongs to a family of related cofactors and is targeted by different classes of activator to mediate transcriptional stimulation...
  22. ncbi The USA-derived transcriptional coactivator PC2 is a submodule of TRAP/SMCC and acts synergistically with other PCs
    S Malik
    Laboratory of Biochemistry and Molecular Biology, The Rockefeller University, New York, New York 10021, USA
    Mol Cell 5:753-60. 2000
    ....
  23. ncbi Phosphorylation of transcriptional coactivator peroxisome proliferator-activated receptor (PPAR)-binding protein (PBP). Stimulation of transcriptional regulation by mitogen-activated protein kinase
    Parimal Misra
    Department of Pathology, The Feinberg School of Medicine, Northwestern University, Chicago, Illinois 60611 3008, USA
    J Biol Chem 277:48745-54. 2002
    Peroxisome proliferator-activated receptor (PPAR)-binding protein (PBP) is an important coactivator for PPARgamma and other transcription factors...
  24. pmc Differential regulation of glucocorticoid receptor transcriptional activation via AF-1-associated proteins
    A B Hittelman
    Department of Microbiology and the Kaplan Comprehensive Cancer Center, NYU School of Medicine, 550 First Avenue, New York, NY 10016, USA
    EMBO J 18:5380-8. 1999
    ..In addition, we show that another member of the DRIP complex, DRIP205, interacts with the GR ligand binding domain in a hormone-dependent manner and facilitates GR transactivation in ..
  25. ncbi The activation function-1 domain of Nur77/NR4A1 mediates trans-activation, cell specificity, and coactivator recruitment
    K D Senali Abayratna Wansa
    University of Queensland Centre for Molecular and Cellular Biology Institute for Molecular Bioscience, St Lucia, Queensland 4072, Australia
    J Biol Chem 277:33001-11. 2002
    ..Thus, the AF-1 domain plays a major role in Nur77-mediated transcriptional activation, cofactor recruitment, and intra- and intermolecular interactions...
  26. pmc The TRAP220 component of a thyroid hormone receptor- associated protein (TRAP) coactivator complex interacts directly with nuclear receptors in a ligand-dependent fashion
    C X Yuan
    Laboratory of Biochemistry and Molecular Biology, The Rockefeller University, New York, NY 10021, USA
    Proc Natl Acad Sci U S A 95:7939-44. 1998
    ..Both TRAP220 and TRAP100 contain LXXLL domains found in other nuclear receptor-interacting proteins and both appear to reside ..
  27. ncbi BRCA1 function mediates a TRAP/DRIP complex through direct interaction with TRAP220
    Osamu Wada
    Institute of Molecular and Cellular Biosciences, University of Tokyo, Yayoi 1 1 1, Bunkyo ku, Tokyo 113 0034, Japan
    Oncogene 23:6000-5. 2004
    ..HeLa S3 nuclear extracts, we isolated BRCT-associated complexes and identified one of the purified components as TRAP220. We then performed interaction studies in vivo (co-immunoprecipitation) and in vitro (glutathione S-transferase ..
  28. ncbi RB18A, whose gene is localized on chromosome 17q12-q21.1, regulates in vivo p53 transactivating activity
    R Frade
    Immunochimie des Régulations Cellulaires et des Interactions Virales, Institut National de la Santé et de la Recherche Médicale INSERM U 354, Centre INSERM, H pital Saint Antoine, Paris, France
    Cancer Res 60:6585-9. 2000
    ..Drane et al., Oncogene, 15: 3013-3024, 1997) RB18A, a new gene whose encoded Mr 205,000 protein interacted in vitro, through its COOH-terminal domain, with p53...
  29. ncbi Acetylation of poly(ADP-ribose) polymerase-1 by p300/CREB-binding protein regulates coactivation of NF-kappaB-dependent transcription
    Paul O Hassa
    Institute of Veterinary Biochemistry and Molecular Biology, University of Zurich, Winterthurerstrasse 190, 8057 Zurich, Switzerland
    J Biol Chem 280:40450-64. 2005
    ..Thus, acetylation of PARP-1 by p300/CREB-binding protein plays an important regulatory role in NF-kappaB-dependent gene activation by enhancing its functional interaction with p300 and the Mediator complex...
  30. ncbi Distinct roles of the steroid receptor coactivator 1 and of MED1 in retinoid-induced transcription and cellular differentiation
    Sébastien Flajollet
    INSERM U459, FacultédeMédecine Henri Warembourg, Lille F 59045, France
    J Biol Chem 281:20338-48. 2006
    ..Unexpectedly, Med1/TRAP220/DRIP205-depleted cells exhibited an exacerbated response to retinoids, both in terms transcriptional responses ..
  31. pmc Ligand induction of a transcriptionally active thyroid hormone receptor coactivator complex
    J D Fondell
    Laboratory of Biochemistry and Molecular Biology, Rockefeller University, New York, NY 10021, USA
    Proc Natl Acad Sci U S A 93:8329-33. 1996
    ..These findings demonstrate the ligand-dependent in vivo formation of a transcriptionally active TR-multisubunit protein complex and suggest a role for TRAPs as positive coactivators for gene-specific transcriptional activation...
  32. ncbi p300 mediates functional synergism between AF-1 and AF-2 of estrogen receptor alpha and beta by interacting directly with the N-terminal A/B domains
    Y Kobayashi
    Institute of Molecular and Cellular Biosciences, The University of Tokyo, Bunkyo-ku, Tokyo 113-0032, Japan
    J Biol Chem 275:15645-51. 2000
    ..proteins and thyroid hormone receptor-associated protein-220/(vitamin D3 receptor-interacting protein- 205-(TRAP220/DRIP205) on the AF-1 activity in terms of synergism with the AF-2 function...
  33. ncbi The TRAP/SMCC/Mediator complex and thyroid hormone receptor function
    M Ito
    Laboratory of Biochemistry and Molecular Biology, The Rockefeller University, 1230 York Avenue, New York, NY 10021-6399, USA
    Trends Endocrinol Metab 12:127-34. 2001
    ..The TRAP220 subunit mediates ligand-dependent interactions of the complex with TR and other nuclear receptors; and genetic ..
  34. ncbi [A case of penicillin-resistant pneumococcal pneumonia and penicillin-binding proteins of the clinical isolates]
    H Shigeno
    Second Department of Internal Medicine, Medical College of Oita
    Kansenshogaku Zasshi 66:508-15. 1992
    ..8%). The CZX resistance was observed only in the present case. The detection of penicillin-binding protein (PBP) and binding affinity of beta-lactam antibiotics were studied using PCG sensitive strain, S...
  35. ncbi Functional interactions between the estrogen receptor and DRIP205, a subunit of the heteromeric DRIP coactivator complex
    D Burakov
    Cell Biology Program, Memorial Sloan Kettering Cancer Center, Sloan Kettering Division, Cornell University, Graduate School of Medical Sciences, New York, New York 10021, USA
    J Biol Chem 275:20928-34. 2000
    ..It is recruited to the receptor in response to ligand through specific interactions of one subunit, DRIP205. We present evidence that DRIP205 interacts with another member of the steroid receptor subfamily, estrogen ..
  36. ncbi Phenotypes and genes of resistance of pneumococci to penicillin isolated from children
    V Kotevska
    Microbiology and Parasitology Institute, Medical Faculty, Ss Cyril and Methodius University, Skopje, R Macedonia
    Prilozi 30:143-54. 2009
    ..0 microg/ml) and susceptible (< 0,06 microg/ml). The genes pbp2b and pbp 2x, which are the genes mainly responsible for the onset of PRP, were detected using polymerase chain reaction (PCR)..
  37. ncbi The catalytic, glycosyl transferase and acyl transferase modules of the cell wall peptidoglycan-polymerizing penicillin-binding protein 1b of Escherichia coli
    M Terrak
    Centre d Ingénierie des Protéines, Universite de Liege, Institut de Chimie, B6, B 4000 Sart Tilman Liège, Belgium
    Mol Microbiol 34:350-64. 1999
    The penicillin-binding protein (PBP) 1b of Escherichia coli catalyses the assembly of lipid-transported N-acetyl glucosaminyl-beta-1, 4-N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-(L)-meso-diaminopimelyl+ ++- (L)-D-alanyl-D-alanine ..
  38. ncbi 1,25-Dihydroxyvitamin D3 stimulates cyclic vitamin D receptor/retinoid X receptor DNA-binding, co-activator recruitment, and histone acetylation in intact osteoblasts
    Sungtae Kim
    Department of Biochemistry, University of Wisconsin Madison, Madison, WI 53706, USA
    J Bone Miner Res 20:305-17. 2005
    ....
  39. doi Ceftaroline: a novel broad-spectrum cephalosporin with activity against meticillin-resistant Staphylococcus aureus
    George G Zhanel
    Department of Medical Microbiology, Faculty of Medicine, University of Manitoba, Winnipeg, Manitoba, Canada
    Drugs 69:809-31. 2009
    ..Ceftaroline has the ability to bind to penicillin-binding protein (PBP)2a, an MRSA-specific PBP that has low affinity for most other beta-lactam antibacterials...
  40. ncbi Peroxisome proliferator-activated receptors, coactivators, and downstream targets
    C Qi
    Department of Pathology, Northwestern University Medical School, Chicago, Illinois 60611, USA
    Cell Biochem Biophys 32:187-204. 2000
    ..system, the authors recently cloned mouse steroid receptor coactivator-1 (SRC-1) and PPAR-binding protein (PBP), and identified them as PPAR coactivators...
  41. ncbi Reactivity of penicillin-binding proteins with peptidoglycan-mimetic beta-lactams: what's wrong with these enzymes?
    Helen R Josephine
    Department of Chemistry, Wesleyan University, Middletown, Connecticut 06459, USA
    Biochemistry 45:15873-83. 2006
    ..kinetics of their inhibition of a panel of penicillin-binding proteins spanning the major classes (Escherichia coli PBP 2 and PBP 5, Streptococcus pneumoniae PBP 1b, PBP 2x and PBP 3, the Actinomadura R39 DD-peptidase, and the ..
  42. pmc Target for bacteriostatic and bactericidal activities of beta-lactam antibiotics against Escherichia coli resides in different penicillin-binding proteins
    G Satta
    Istituto di Microbiologia, Universita Cattolica del Sacro Cuore, Rome, Italy
    Antimicrob Agents Chemother 39:812-8. 1995
    The relationship between cell-killing kinetics and penicillin-binding protein (PBP) saturation has been evaluated in the permeability mutant Escherichia coli DC2 in which the antimicrobial activity of beta-lactams has been described as ..
  43. ncbi Decreased affinity of mosaic-structure recombinant penicillin-binding protein 2 for oral cephalosporins in Neisseria gonorrhoeae
    Susumu Ochiai
    Research and Development Department, Mitsubishi Kagaku Bio Clinical Laboratories, Inc, 3 30 1 Shimura, Itabashi ku, Tokyo 174 8555, Japan
    J Antimicrob Chemother 60:54-60. 2007
    In Neisseria gonorrhoeae, the mosaic structure of penicillin-binding protein 2 (PBP 2), composed of fragments of PBP 2 from Neisseria cinerea and Neisseria perflava, was significantly associated with decreased susceptibility to ..
  44. pmc The highly conserved serine threonine kinase StkP of Streptococcus pneumoniae contributes to penicillin susceptibility independently from genes encoding penicillin-binding proteins
    Ricardo Dias
    Department of Infectious Diseases, National Institute of Health Dr Ricardo Jorge, Lisbon, Portugal
    BMC Microbiol 9:121. 2009
    ..with a N-terminal kinase domain a C-terminal PASTA domain carrying the signature of penicillin-binding protein (PBP) and prokaryotic serine threonine kinase...
  45. pmc Rapid detection of the mosaic structure of the Neisseria gonorrhoeae penA Gene, which is associated with decreased susceptibilities to oral cephalosporins
    Susumu Ochiai
    Advanced Medicine Development Department, R and D and Business Development Segment, Mitsubishi Chemical Medience Corporation, 3 30 1 Shimura, Itabashi ku, Tokyo 174 8555, Japan
    J Clin Microbiol 46:1804-10. 2008
    In Neisseria gonorrhoeae, the mosaic structure of the penA gene (encoding penicillin-binding protein 2 [PBP 2]), which is composed of fragments of the penA genes from Neisseria cinerea and Neisseria perflava, has been significantly ..
  46. ncbi Synthetic progestins stimulate prostatic binding protein messenger RNAs in the rat ventral prostate
    C Labrie
    Medical Research Council Group in Molecular Endocrinology, Laval University Medical Center, Quebec, Canada
    Mol Cell Endocrinol 68:169-79. 1990
    ..markers of androgen action in the rat ventral prostate, namely the levels of messenger RNAs encoding the C1 (PBP-C1) and C3 (PBP-C3) components of rat prostatic binding protein (PBP)...
  47. pmc Analysis of penicillin-binding protein genes of clinical isolates of Streptococcus pneumoniae with reduced susceptibility to amoxicillin
    Mignon du Plessis
    Pneumococcal Diseases Research Unit of the Medical Research Council, National Health Laboratory Service and University of the Witwatersrand, de Korte Street, Hillbrow, PO Box 1038, Johannesburg 2000, South Africa
    Antimicrob Agents Chemother 46:2349-57. 2002
    ..resistance phenotype of higher amoxicillin MICs in relation to the penicillin MICs prompted an analysis of the pbp genes from three such strains isolated in France...
  48. doi Investigation of the mechanism of the cell wall DD-carboxypeptidase reaction of penicillin-binding protein 5 of Escherichia coli by quantum mechanics/molecular mechanics calculations
    Qicun Shi
    Department of Chemistry and Biochemistry, University of Notre Dame, Notre Dame, Indiana 46556, USA
    J Am Chem Soc 130:9293-303. 2008
    Penicillin-binding protein 5 (PBP 5) of Escherichia coli hydrolyzes the terminal D-Ala-D-Ala peptide bond of the stem peptides of the cell wall peptidoglycan...
  49. pmc Complete sequences of six penicillin-binding protein genes from 40 Streptococcus pneumoniae clinical isolates collected in Japan
    Yumiko Sanbongi
    Pharmaceutical Research Department, Meiji Seika Kaisha Ltd, 760 Morooka cho, Kohoku Ku, Yokohama 222 8567, Japan
    Antimicrob Agents Chemother 48:2244-50. 2004
    All six penicillin-binding protein (PBP) genes, namely, pbp1a, pbp1b, pbp2a, pbp2b, pbp2x, and pbp3, of 40 Streptococcus pneumoniae clinical isolates, including penicillin-resistant S...
  50. ncbi Non-Penicillin-Binding protein mediated high-level penicillin and cephalosporin resistance in a Hungarian clone of Streptococcus pneumoniae
    A M Smith
    Department of Clinical Microbiology and Infectious Diseases, South African Institute for Medical Research, Johannesburg
    Microb Drug Resist 6:105-10. 2000
    ..The role that each penicillin-binding protein (PBP) plays in the development of resistance in these strains was investigated via transformation of susceptible strain ..
  51. pmc Association of amino acid substitutions in penicillin-binding protein 3 with beta-lactam resistance in beta-lactamase-negative ampicillin-resistant Haemophilus influenzae
    K Ubukata
    Pharmaceutical Research Center, Meiji Seika Kaisha, Ltd, 760 Morookacho, Kohoku Ku, Yokohama, Japan
    Antimicrob Agents Chemother 45:1693-9. 2001
    The affinity of [(3)H]benzylpenicillin for penicillin-binding protein (PBP) 3A was reduced in 25 clinical isolates of beta-lactamase-negative ampicillin (AMP)-resistant (BLNAR) Haemophilus influenzae for which the AMP MIC was > or =1...
  52. ncbi In vitro activity, beta-lactamase stability and PBP affinity of RU 51,746-2, the active metabolite of the new orally absorbed cephalosporin ester, RU 51807
    M Boaretti
    Istituto di Microbiologia, , Italy
    J Chemother 3:57-61. 1991
    ..RU 51,746-2 showed a good affinity for high molecular weight penicillin binding proteins (PBPs) of E. coli. PBP 3 was found to be the target for growth inhibition, whereas the additional saturation of PBP 1 and 2 was required ..
  53. ncbi [Meningitis due to streptococcus viridans]
    D Alba
    Departamento de Medicina Interna, Hospital La Paz, Paseo de la Castellana, Madrid
    Rev Neurol 23:656-60. 1995
    ..VS are normally sensitive to penicillin G. However VS may be resistant to penicillin (low affinity of PBP to penicillin) and cause serious infections, including meningitis...
  54. pmc Genetics of chromosomally mediated intermediate resistance to ceftriaxone and cefixime in Neisseria gonorrhoeae
    Shuqing Zhao
    Department of Pharmacology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599 7365, USA
    Antimicrob Agents Chemother 53:3744-51. 2009
    ..Ceph(i)] strains) contain a mosaic penA allele encoding penicillin-binding protein 2 (PBP 2) with nearly 60 amino acid differences compared to the sequence of wild-type PBP 2, together with a set of ..
  55. doi Biogenesis of phycobiliproteins: I. cpcS-I and cpcU mutants of the cyanobacterium Synechococcus sp. PCC 7002 define a heterodimeric phyococyanobilin lyase specific for beta-phycocyanin and allophycocyanin subunits
    Gaozhong Shen
    Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, Pennsylvania 16802, USA
    J Biol Chem 283:7503-12. 2008
    ..Single and double mutant strains for cpcS-I and cpcU exhibited slower growth rates, reduced PBP levels, and impaired assembly of phycobilisomes, but a cpcV mutant had no discernable phenotype...
  56. pmc Diversity of beta-lactam resistance-conferring amino acid substitutions in penicillin-binding protein 3 of Haemophilus influenzae
    Henri Dabernat
    Laboratoire de Microbiologie, Centre national de reference des Haemophilus influenzae, Toulouse, France
    Antimicrob Agents Chemother 46:2208-18. 2002
    The sequences of the ftsI gene, encoding the transpeptidase domain of penicillin binding protein (PBP) 3A and/or PBP 3B, which are involved in septal peptidoglycan synthesis, were determined for 108 clinical strains of Haemophilus ..
  57. doi Structure of PBP-A from Thermosynechococcus elongatus, a penicillin-binding protein closely related to class A beta-lactamases
    Carole Urbach
    Laboratoire d Ingénierie des Protéines et des Peptides, Institut des Sciences de la Vie, Universite Catholique de Louvain, Place Croix du Sud 4 5, bte3, 1348 Louvain la Neuve, Belgium
    J Mol Biol 386:109-20. 2009
    ..With the aim of evolving a member of this family into a beta-lactamase, PBP-A from Thermosynechococcus elongatus has been chosen because of its thermostability...
  58. pmc Altering enzymatic activity: recruitment of carboxypeptidase activity into an RTEM beta-lactamase/penicillin-binding protein 5 chimera
    Y H Chang
    Department of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena 91125
    Proc Natl Acad Sci U S A 87:2823-7. 1990
    ..into a beta-lactamase background, we have prepared a chimeric protein by inserting a 28-amino acid segment of PBP-5 of Escherichia coli in place of the corresponding region of the RTEM-1 beta-lactamase...
  59. ncbi Alterations in penicillin binding protein gene of Streptococcus pneumoniae and their correlation with susceptibility patterns
    Yoshinobu Ohsaki
    First Department of Medicine, Asahikawa Medical College, 2 1 1 1 Midorigaoka Higashi, Asahikawa 078 8510, Hokkaido, Japan
    Int J Antimicrob Agents 22:140-6. 2003
    Penicillin binding protein (pbp) gene alterations of 328 clinical isolates of Streptococcus pneumoniae were examined for a correlation with their antibiotic-resistance...
  60. doi Single dose 1 g ceftriaxone for urogenital and pharyngeal infection caused by Neisseria gonorrhoeae
    Tetsuro Muratani
    Department of Urology, Asian Clinical Research Group, School of Medicine, University of Occupational and Environmental Health, Kitakyushu, Fukuoka, Japan
    Int J Urol 15:837-42. 2008
    ..gonorrhoeae) including the oral cephem-resistant strain with chimera penicillin-binding protein 2 (PBP-2) (cefozopran-resistant N. gonorrhoeae, CZRNG).
  61. ncbi Continuous administration of PBP-2- and PBP-3-specific beta-lactams causes higher cytokine responses in murine Pseudomonas aeruginosa and Escherichia coli sepsis
    Jacqueline Buijs
    Department of Medical Microbiology and Infectious Diseases, Canisius Wilhelmina Hospital, Nijmegen, The Netherlands
    J Antimicrob Chemother 59:926-33. 2007
    ..High-dose ceftazidime, which inhibits penicillin-binding protein (PBP)-1 in Gram-negative bacteria, causes quick bacteriolysis and low endotoxin release...
  62. ncbi Evaluation of bactericidal activity of cefotaxime and other beta-lactams by a novel method
    G Satta
    Istituto di Microbiologia dell Università di Siena
    Drugs 35:35-40. 1988
    ..first evaluated the kinetics of cellular killing by various beta-lactams as related to penicillin-binding-protein (PBP) binding in Escherichia coli DC2, a hyperpermeable mutant. We concluded that in E...
  63. doi The predominance of IgG4 in prodromal bullous pemphigoid
    Philina M Lamb
    Department of Dermatology, University of California, Davis, Sacramento, California, USA
    Int J Dermatol 47:150-3. 2008
    Prodromal bullous pemphigoid (PBP) and bullous pemphigoid (BP) demonstrate immunoglobulin G (IgG) and/or C3 deposition at the basement membrane zone (BMZ) on direct immunofluorescence...
  64. ncbi Penicillin-binding protein 1B from Escherichia coli contains a membrane association site in addition to its transmembrane anchor
    R A Nicholas
    University of North Carolina, Department of Pharmacology, Chapel Hill 27599 7365
    J Biol Chem 268:5632-41. 1993
    A working structural model of penicillin-binding protein 1B (PBP 1B) from Escherichia coli derived from previous data consists of a highly charged aminoterminal cytoplasmic tail, a 23-amino-acid hydrophobic transmembrane anchor, and a ..
  65. pmc First characterization of heterogeneous resistance to imipenem in invasive nontypeable Haemophilus influenzae isolates
    Marina Cerquetti
    Department of Infectious, Parasitic and Immune Mediated Diseases, Istituto Superiore di Sanita, Viale Regina Elena 299, 00161 Roma, Italy
    Antimicrob Agents Chemother 51:3155-61. 2007
    ..The sequencing of the ftsI gene encoding penicillin-binding protein 3 (PBP 3) and comparison with the sequence of the imipenem-susceptible H...
  66. pmc Escherichia coli mutants lacking all possible combinations of eight penicillin binding proteins: viability, characteristics, and implications for peptidoglycan synthesis
    S A Denome
    Department of Microbiology and Immunology, School of Medicine, University of North Dakota, Grand Forks, North Dakota 58202 9037, USA
    J Bacteriol 181:3981-93. 1999
    ..these proteins make to the physiology of Escherichia coli, we constructed 192 mutants from which eight PBP genes were deleted in every possible combination...
  67. pmc Association of a thr-371 substitution in a conserved amino acid motif of penicillin-binding protein 1A with penicillin resistance of Streptococcus pneumoniae
    Y Asahi
    Department of Clinical Pathology, Teikyo University School of Medicine, 2 11 1 Kaga, Itabashi ku, Tokyo 173 8605, Japan
    Antimicrob Agents Chemother 42:2267-73. 1998
    ..We determined the nucleotide sequence between 1,903 and 3,097 bp of pbp1a, which encodes the transpeptidase domain of PBP 1A, from clinical isolates of penicillin-resistant Streptococcus pneumoniae (PRSP) serotypes 19 (n = 8), 6 (n = 9), ..
  68. pmc Crystal structures of covalent complexes of β-lactam antibiotics with Escherichia coli penicillin-binding protein 5: toward an understanding of antibiotic specificity
    George Nicola
    Department of Biochemistry, Medical University of South Carolina, Charleston, South Carolina 29425, USA
    Biochemistry 49:8094-104. 2010
    ..We have determined crystal structures of Escherichia coli PBP 5 as covalent complexes with imipenem, cloxacillin, and cefoxitin...
  69. ncbi On the relationships between molecular conformation, affinity towards penicillin-binding proteins, and biological activity of penicillin G-sulfoxide
    F Beise
    Robert Koch Institute, Federal Health Organization, Berlin, Bundesrepublik Deutschland
    Z Naturforsch C 43:656-64. 1988
    The binding capacity of penicillin G-sulfoxide towards the penicillin-binding proteins (PBP) of Staphylococcus aureus H was studied...
  70. pmc Amino acid mutations essential to production of an altered PBP 2X conferring high-level beta-lactam resistance in a clinical isolate of Streptococcus pneumoniae
    Anthony M Smith
    Respiratory and Meningeal Pathogens Research Unit, National Institute for Communicable Diseases, Medical Research Council, Johannesburg, South Africa
    Antimicrob Agents Chemother 49:4622-7. 2005
    Altered penicillin-binding protein 2X (PBP 2X) is a primary beta-lactam antibiotic resistance determinant and is essential to the development of penicillin and cephalosporin resistance in the pneumococcus...
  71. ncbi Presence of international multiresistant clones of Streptococcus pneumoniae in Colombia
    M C Vela
    Grupo de Microbiologia, Instituto Nacional de Salud, Bogota, Colombia
    Microb Drug Resist 7:153-64. 2001
    ..serotype 23F isolates were grouped in two clusters: 15 (31%) multiresistant isolates showed PFGE pattern A and PBP I profile, thus making them indistinguishable from Spain23F-1 clone, and 34 (69%) with PFGE pattern C, PBP II ..
  72. ncbi Investigation of the substrate spectrum of the human mismatch-specific DNA N-glycosylase MED1 (MBD4): fundamental role of the catalytic domain
    F Petronzelli
    Fox Chase Cancer Center, Philadelphia, Pennsylvania, USA
    J Cell Physiol 185:473-80. 2000
    The human DNA repair protein MED1 (also known as MBD4) was isolated as an interactor of the mismatch repair protein MLH1 in a yeast two-hybrid screening...
  73. pmc Endopeptidase penicillin-binding proteins 4 and 7 play auxiliary roles in determining uniform morphology of Escherichia coli
    Bernadette M Meberg
    Department of Microbiology and Immunology, University of North Dakota School of Medicine, Grand Forks, ND 58202 9037, USA
    J Bacteriol 186:8326-36. 2004
    The low-molecular-weight (LMW) penicillin-binding protein, PBP 5, plays a dominant role in determining the uniform cell shape of Escherichia coli...
  74. ncbi The mechanism of staphylococci resistance to methicillin: a critical analysis of dominant opinions
    G Satta
    Institute of Microbiology of the University of Siena, Italy
    J Chemother 3:144-8. 1991
    ..to methicillin has been reported to be constantly associated with production of a novel penicillin-binding protein (PBP), indicated as PBP2a...
  75. doi Mutational analysis of the substrate specificity of Escherichia coli penicillin binding protein 4
    Thomas B Clarke
    Department of Biological Sciences, University of Warwick, Coventry, UK
    Biochemistry 48:2675-83. 2009
    Escherichia coli PBP4 is the archetypal class C, low molecular mass penicillin binding protein (LMM-PBP) and possesses both dd-carboxypeptidase and dd-endopeptidase activity...
  76. ncbi Site-directed mutants of a soluble form of penicillin-binding protein 5 from Escherichia coli and their catalytic properties
    R A Nicholas
    Department of Biochemistry and Molecular Biology, Harvard University, Cambridge, Massachusetts 02138
    J Biol Chem 263:2034-40. 1988
    ..protein 5 (sPBP 5) from Escherichia coli were produced in large amounts by placing the dacA gene that encodes PBP 5 under the control of the trp-lac fusion promoter...
  77. ncbi Mosaic pbpX genes of major clones of penicillin-resistant Streptococcus pneumoniae have evolved from pbpX genes of a penicillin-sensitive Streptococcus oralis
    C Sibold
    Max Planck Institut fur Molekulare Genetik, Berlin, Germany
    Mol Microbiol 12:1013-23. 1994
    Penicillin-resistant clinical isolates of Streptococcus pneumoniae contain mosaic penicillin-binding protein (PBP) genes that encode PBPs with decreased affinity for beta-lactam antibiotics...
  78. pmc Restoration of susceptibility of methicillin-resistant Staphylococcus aureus to beta-lactam antibiotics by acidic pH: role of penicillin-binding protein PBP 2a
    Sandrine Lemaire
    Unité de Pharmacologie Cellulaire et Moléculaire, Universite Catholique de Louvain, B 1200 Brussels, Belgium
    J Biol Chem 283:12769-76. 2008
    ..The central factor for the MRSA phenotype is the function of the penicillin-binding protein (PBP) 2a, which maintains transpeptidase activity while being poorly inhibited by beta-lactams because of a closed ..
  79. pmc Discrete roles for peroxisome proliferator-activated receptor gamma and retinoid X receptor in recruiting nuclear receptor coactivators
    W Yang
    Cell Biology Program, Memorial Sloan Kettering Cancer Center, New York, New York 10021, USA
    Mol Cell Biol 20:8008-17. 2000
    ..A single DRIP subunit, DRIP205 (TRAP220, PBP), binds directly to PPARgamma...
  80. pmc Mecillinam resistance in Escherichia coli is conferred by loss of a second activity of the AroK protein
    D Vinella
    Laboratory of Molecular Genetics, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892 2785, USA
    J Bacteriol 178:3818-28. 1996
    Mecillinam, a beta-lactam antibiotic specific to penicillin-binding protein 2 (PBP 2) in Escherichia coli, blocks cell wall elongation and, indirectly, cell division, but its lethality can be overcome by increased levels of ppGpp, the ..
  81. pmc Highly variable penicillin resistance determinants PBP 2x, PBP 2b, and PBP 1a in isolates of two Streptococcus pneumoniae clonal groups, Poland 23F-16 and Poland 6B-20
    Radoslaw Izdebski
    National Medicines Institute, Division of Clinical Microbiology and Infection Prevention, Chełmska 30 34, Warsaw 00 725, Poland
    Antimicrob Agents Chemother 52:1021-7. 2008
    ..pneumoniae clonal groups that are prevalent in Poland, Poland 23F-16 and Poland 6B-20, were investigated by PBP profile analysis, antibody reactivity pattern analysis, and DNA sequence analysis of the transpeptidase (TP) domain-..
  82. doi Comparative genome analysis of high-level penicillin resistance in Streptococcus pneumoniae
    Amelia G Tait-Kamradt
    Antibacterial Biology, Pfizer Global Research and Development, Groton, Connecticut, USA
    Microb Drug Resist 15:69-75. 2009
    ..Previous studies have traced the changes in penicillin binding proteins (PBP) that result in decreased penicillin susceptibility, and the role of several PBP genes in high-level resistance...
  83. ncbi Crystal structure of the 47-kDa lipoprotein of Treponema pallidum reveals a novel penicillin-binding protein
    Ranjit K Deka
    Departments of Microbiology and Biochemistry, University of Texas Southwestern Medical Center, Dallas 75390, USA
    J Biol Chem 277:41857-64. 2002
    ..We previously identified the T. pallidum 47-kDa lipoprotein (Tp47) as a penicillin-binding protein (PBP)...
  84. ncbi Extract of Pelargonium sidoides (EPs 7630) improves phagocytosis, oxidative burst, and intracellular killing of human peripheral blood phagocytes in vitro
    Andreas Conrad
    Institute of Environmental Medicine and Hospital Epidemiology, University Medical Center Freiburg, Freiburg, Germany
    Phytomedicine 14:46-51. 2007
    ..objective of this study was to examine the impact of EPs 7630 on the activity of human peripheral blood phagocytes (PBP). A whole blood-based, flow cytometric assay was used to simultaneously assess phagocytosis and oxidative burst...
  85. pmc Contributions of PBP 5 and DD-carboxypeptidase penicillin binding proteins to maintenance of cell shape in Escherichia coli
    D E Nelson
    Department of Microbiology and Immunology, University of North Dakota School of Medicine and Health Sciences, Grand Forks, ND 58202 9037, USA
    J Bacteriol 183:3055-64. 2001
    ..by investigating the effects of these proteins on the aberrant morphology of DeltadacA mutants, which produce no PBP 5...
  86. pmc Nasopharyngeal Haemophilus influenzae carriage in Japanese children attending day-care centers
    Koichi Hashida
    Department of Otorhinolaryngology, School of Medicine, University of Occupational and Environmental Health, 1 1 Iseigaoka, Yahatanishi ku, Kitakyushu 807 8555, Japan
    J Clin Microbiol 46:876-81. 2008
    ..and analyzed them by antimicrobial susceptibility testing, PCR for bla(TEM-1) and the penicillin-binding protein (PBP) gene, and pulsed-field gel electrophoresis (PFGE). The overall carriage rate was 47.4% (172/363), and 37...
  87. pmc Reconstruction of the phenotypes of methicillin-resistant Staphylococcus aureus by replacement of the staphylococcal cassette chromosome mec with a plasmid-borne copy of Staphylococcus sciuri pbpD gene
    Aude Antignac
    The Rockefeller University, New York, NY 10021, USA
    Antimicrob Agents Chemother 53:435-41. 2009
    ..carry a close homologue of mecA in the form of pbpD, the genetic determinant of penicillin binding protein 4 (PBP 4) of S. sciuri...
  88. ncbi Use of a beta-lactamase fusion vector to investigate the organization of penicillin-binding protein 1B in the cytoplasmic membrane of Escherichia coli
    A Edelman
    Microbial Genetics Group, School of Biological Sciences, University of Sussex, Falmer, Brighton, UK
    Mol Microbiol 1:101-6. 1987
    ..of TEM beta-lactamase was fused to random positions within the coding region of the penicillin-binding protein 1B (PBP 1B) gene and the nucleotide sequences across the fusion junctions of 100 in-frame fusions were determined...
  89. doi Concentration-dependency of beta-lactam-induced filament formation in Gram-negative bacteria
    J Buijs
    Department of Medical Microbiology and Infectious Diseases, Atrium Medical Centre, Heerlen, The Netherlands
    Clin Microbiol Infect 14:344-9. 2008
    Ceftazidime and cefotaxime are beta-lactam antibiotics with dose-related affinities for penicillin-binding protein (PBP)-3 and PBP-1. At low concentrations, these antibiotics inhibit PBP-3, leading to filament formation...
  90. pmc Diversity of penA alterations and subtypes in Neisseria gonorrhoeae strains from Sydney, Australia, that are less susceptible to ceftriaxone
    David M Whiley
    Queensland Paediatric Infectious Diseases Laboratory, Sir Albert Sakzewski Virus Research Centre, Royal Children s Hospital and Health Service District, Brisbane, Queensland, Australia
    Antimicrob Agents Chemother 51:3111-6. 2007
    ..In this study, we examined the complete penicillin-binding protein 2 (PBP 2) amino acid sequences of 109 gonococci, selected on the basis of their diverse temporal and geographic origins ..
  91. pmc Induction of the major integral membrane protein of mouse liver peroxisomes by peroxisome proliferators
    N Chen
    Division of Science and Technology, Griffith University, Brisbane, Queensland, Australia
    Biochem J 283:605-10. 1992
    ..PMP68) by a number of these agents, and compared this with their effect on the peroxisomal bifunctional protein (PBP), an enzyme of the beta-oxidation pathway which is located in the peroxisome matrix...
  92. ncbi Enhancement of acid tolerance in Zymomonas mobilis by a proton-buffering peptide
    David J Baumler
    Cellular and Molecular Biology, University of Wisconsin, 1925 Willow Drive, Madison, 53706 1187, USA
    Appl Biochem Biotechnol 134:15-26. 2006
    A portion of the cbpA gene from Escherichia coli K-12 encoding a 24 amino acid proton-buffering peptide (Pbp) was cloned via the shuttle vector pJB99 into E. coli JM105 and subsequently into Zymomonas mobilis CP4...
  93. pmc A conserved Mediator-CDK8 kinase module association regulates Mediator-RNA polymerase II interaction
    Kuang Lei Tsai
    Department of Cell Biology, Scripps Research Institute, La Jolla, California, USA
    Nat Struct Mol Biol 20:611-9. 2013
    ....
  94. ncbi 3D-RISM-KH molecular theory of solvation and density functional theory investigation of the role of water in the aggregation of model asphaltenes
    L M da Costa
    National Institute for Nanotechnology, National Research Council of Canada, 11421 Saskatchewan Drive, Edmonton, Alberta, T6G 2M9, Canada
    Phys Chem Chem Phys 14:3922-34. 2012
    ..the role of water in aggregation of the asphaltene model compound 4,4'-bis(2-pyren-1-yl-ethyl)-2,2'-bipyridine (PBP) [X. Tan, H. Fenniri and M. R. Gray, Energy Fuels, 2008, 22, 715]...
  95. ncbi Potential transition state analogue inhibitors for the penicillin-binding proteins
    Aleksandr Pechenov
    Division of Pharmaceutical Sciences, University of Missouri Kansas City, 5005 Rockhill Road, Kansas City, Missouri 64110, USA
    Biochemistry 42:579-88. 2003
    ..The development of new PBP inhibitors is a potentially viable strategy for developing new antibacterial agents...
  96. pmc Ligand-dependent degradation of retinoid X receptors does not require transcriptional activity or coactivator interactions
    D L Osburn
    Nuclear Receptor Discovery, Ligand Pharmaceuticals, San Diego, California 92121, USA
    Mol Cell Biol 21:4909-18. 2001
    ..Taken together, the data suggest a model for targeted destruction of transcription factors based on structural or conformational signals as opposed to functional coupling with gene transcription...
  97. doi Activator-mediator binding stabilizes RNA polymerase II orientation within the human mediator-RNA polymerase II-TFIIF assembly
    Carrie Bernecky
    Department of Chemistry and Biochemistry, University of Colorado, Boulder, CO 80309, USA
    J Mol Biol 417:387-94. 2012
    ..Because Mediator interacts extensively with pol II, these results suggest that Mediator structural shifts induced by activator binding help stably orient pol II prior to transcription initiation...
  98. doi Coupling of different isolated photosynthetic light harvesting complexes and CdSe/ZnS nanocrystals via Förster resonance energy transfer
    F J Schmitt
    Institute of Chemistry, Biophysical Chemistry, Berlin Institute of Technology, Berlin, Germany
    Biochim Biophys Acta 1817:1461-70. 2012
    ..2 (LH1 and LH2, respectively) from purple bacteria, phycobiliproteins (PBPs) from cyanobacteria and the rod-shaped PBP from the cyanobacterium Acaryochloris marina)...
  99. doi Polymeric black tea polyphenols modulate the localization and activity of 12-O-tetradecanoylphorbol-13-acetate-mediated kinases in mouse skin: mechanisms of their anti-tumor-promoting action
    Gaurav Kumar
    Advanced Centre for Treatment, Research and Education in Cancer, Tata Memorial Centre, Kharghar, Navi Mumbai 410210, India
    Free Radic Biol Med 53:1358-70. 2012
    ..However, their mechanisms of action are not fully elucidated. In this study, mechanisms of PBP-mediated antipromoting effects were investigated in a mouse model employing the tumor promoter 12-O-..
  100. doi Cross-talk between HER2 and MED1 regulates tamoxifen resistance of human breast cancer cells
    Jiajun Cui
    Department of Cancer and Cell Biology, University of Cincinnati College of Medicine, Cincinnati, OH 45267, USA
    Cancer Res 72:5625-34. 2012
    ..In this study, we report that the ER coactivator MED1 is a novel cross-talk point for the HER2 and ERα pathways...
  101. pmc Reconstruction of Escherichia coli mrcA (PBP 1a) mutants lacking multiple combinations of penicillin binding proteins
    B M Meberg
    Department of Microbiology and Immunology, University of North Dakota School of Medicine, Grand Forks, North Dakota 58202 9037, USA
    J Bacteriol 183:6148-9. 2001
    Previously, we constructed a set of mutants from which eight penicillin binding protein (PBP) genes were deleted in 192 combinations from Escherichia coli (S. A. Denome, P. K. Elf, T. A. Henderson, D. E. Nelson, and K. D. Young, J...

Research Grants97

  1. Cell wall synthesis enzymes and beta-lactam resistance in Enterococcus faecium
    Louis B Rice; Fiscal Year: 2010
    ..faecium is the expression of low affinity class B penicillin-binding protein Pbp5. As a class B Pbp with only transpeptidase activity, Pbp5 must coordinate its activities with that of glycosyltransferases to ..
  2. Molecular Targets in Peptidoglycan Synthesis
    Christopher Davies; Fiscal Year: 2009
    ..basis for antibiotic resistance will be investigated by structural and biochemical studies of a unique variant of PBP 2 from strains of N. gonorrhoeae with intermediate-level resistance to ceftriaxone...
  3. Molecular Targets in Peptidoglycan Synthesis
    Christopher Davies; Fiscal Year: 2010
    ..basis for antibiotic resistance will be investigated by structural and biochemical studies of a unique variant of PBP 2 from strains of N. gonorrhoeae with intermediate-level resistance to ceftriaxone...
  4. Antibiotic Resistance in the Oral Streptococci
    Jeffrey Rogers; Fiscal Year: 2003
    ..pneumoniae quickly led to the hypothesis that these pbp genes evolved by recombination with homologs from other streptococcal species, in particular the oral streptococci...
  5. B-lactam mediated SOS response and expression of resistance in clinical MRSA
    Adriana E Rosato; Fiscal Year: 2010
    ..to the acquisition of a gene (mecA) that encodes a ?-lactam insensitive target enzyme, penicillin-binding protein (PBP)2a...
  6. Penicillin-Binding Proteins, Mechanism and Inhibition
    Shahriar Mobashery; Fiscal Year: 2004
    ..is already synthesized, are proposed as mechanistic probes for the transpeptidase reaction carried out by certain PBP in the last step of cell wall biosynthesis (cross-linking of cell wall)...
  7. Peptidoglycan Biogenesis in Escherichia Coli
    Thomas G Bernhardt; Fiscal Year: 2010
    ..beyond the "crutch" of antibiotic probes, we developed a genetic approach to identify factors needed for proper PBP function in vivo using E. coli as a model system. E. coli encodes three bi-functional PBPs: PBP1A, PBP1B, and PBP1C...
  8. Molecular Targets in Peptidoglycan Synthesis
    Christopher Davies; Fiscal Year: 2006
    ..coli (PBP 5) that serves as a model system for elucidating PBP function, an essential transpeptidase (PBP 2) from N...
  9. PEPTIDOGLYCAN SYNTHESIS IN BACILLUS SUBTILIS
    DAVID POPHAM; Fiscal Year: 2006
    ..subtilis class A PBP, PBP1, will be assessed...
  10. Peptidomimetics of D-Ala-D-Ala as Novel Antibacterials
    Norma Dunlap; Fiscal Year: 2003
    ..Penicillins are bacterial cell wall synthesis inhibitors and act by inhibition of Penicillin Binding Proteins (PBP's), also know as D-D-peptidases...
  11. STAPHYLOCOCCAL METHICILLIN RESISTANCE LOCUS
    GORDON LEE ARCHER; Fiscal Year: 2011
    ..The gene responsible for methicillin resistance (MR), mecA, encodes a new PBP (PBP2a) that can maintain cell wall structure but is resistant to beta-lactam inhibition mecA is carried on a ..
  12. NEW INHIBITORS FOR THE PENICILLIN BINDING PROTEINS
    WILLIAM GUTHEIL; Fiscal Year: 2002
    ..In preliminary studies precise and sensitive UV-vis based coupled enzyme assays have been established for PBP activity, for both D-Ala and D-Lac based substrates, and used to partially characterize the activity of E...
  13. GONOCOCCI: GENETICS OF RESISTANCE TO PMN PROTEINS
    William Shafer; Fiscal Year: 1993
    ..This enzyme is termed penicillin binding protein 2 (PBP 2) and it gives evidence of being the lethal target of cathepsin G action...
  14. Regulation/mecA gene expression/clinic S.aureus isolates
    Adriana Rosato; Fiscal Year: 2005
    ..by acquiring a gene (mecA) that encodes a beta-lactam insensitive target enzyme, penicillin binding protein (PBP)2a...
  15. RESISTANCE TO BETA-LACTAM ANTIBIOTICS
    Shahriar Mobashery; Fiscal Year: 2010
    ..both beta- lactamases, enzymes that hydrolytically destroy these antibiotics, or a penicillin-binding protein (PBP 2a) that is not readily inhibited by beta-lactam antibiotics. Three specific Aims are proposed...
  16. RESISTANCE TO BETA-LACTAM ANTIBIOTICS
    Shahriar Mobashery; Fiscal Year: 2007
    ..both beta-lactamases, enzymes that hydrolytically destroy these antibiotics, or a penicillin-binding protein (PBP 2a) that is not readily inhibited by beta-lactam antibiotics. Three specific Aims are proposed...
  17. PENICILLIN RESISTANT NEISSERIA GONORRHOEAE (CMRNG)
    Robert Nicholas; Fiscal Year: 2000
    ..The investigator proposes to clone the gene encoding PBP 1 from penicillin-resistant strains, identify the mutations in PBP1 that lead to the decrease in affinity for ..
  18. Vitamin D Receptor Coactivators in Keratinocytes
    Daniel Bikle; Fiscal Year: 2009
    ..Furthermore, DRIP205, the protein in the DRIP complex that binds directly to VDR, is abundantly expressed in proliferating ..
  19. PENICILLIN INTERACTIVE PROTEINS OF STAPHYLOCOCCUS AUREUS
    Henry Chambers; Fiscal Year: 2003
    ..Resistance is mainly due to production a novel low affinity penicillin bind protein, PBP 2a, a well wall synthetic enzyme. PBP 2a seems to substitute for all other PBPs...
  20. ANALYSIS OF TRYPANOSOME MRNA SYNTHESIS BY GENE TRANSFER
    Vivian Bellofatto; Fiscal Year: 2007
    ..in the field of trypanosome gene expression by identifying and beginning to characterize Leptomonas seymouri PBP-1 and PBP-2...
  21. MODULATION OF IFN ACTION VIA NOVEL REGULATORY FACTORS
    DHAN V KALVAKOLANU; Fiscal Year: 2010
    ..Studies during the last funding period also identified Med1, a major subunit of Mediator, an IFN-induced binding partner of C/EBP- 2...
  22. Regulation of Genomic and Epigenomic Stability at CpG Sites
    Alfonso Bellacosa; Fiscal Year: 2010
    The overall goal of this project is to understand how two related mammalian base excision repair enzymes, MED1 and TDG, maintain genomic and epigenomic stability at CpG sites, by preventing both mutations and altered methylation ..
  23. Penicillin-resistant Neisseria gonorrhoeae (CMRNG)
    Robert Nicholas; Fiscal Year: 2005
    ..These include the penA gene encoding altered forms of penicillin-binding protein 2 (PBP 2), the mtr loci conferring resistance to hydrophobic agents, and the penB gene, which decreases outer membrane ..
  24. The MED1 Protein in DNA Damage Response and Repair
    Alfonso Bellacosa; Fiscal Year: 2007
    ..We identified one of these enzymes, human MED1 (also known as MBD4), as an interact or of the mismatch repair (MMR) protein MLH1...
  25. MED1 MUTATIONS IN COLORECTAL CANCER
    Alfonso Bellacosa; Fiscal Year: 2002
    ..In a yeast two-hybrid screening with the mismatch repair protein MLH1 as "bait", we cloned MED1, a novel human DNA repair gene...
  26. Mechanisms of antibiotic action in Neisseria gonorrhoeae.
    Robert Nicholas; Fiscal Year: 2007
    ..we describe a proteomics approach to identify and characterize proteins that bind to penicillin-binding protein 1 (PBP 1), PBP 2, and the lytic transglycosylase MltA, and thus reveal the form and function of the multi-enzyme complexes ..
  27. Mechanisms of antibiotic action in Neisseria gonorrhoeae.
    Robert A Nicholas; Fiscal Year: 2010
    ..we describe a proteomics approach to identify and characterize proteins that bind to penicillin-binding protein 1 (PBP 1), PBP 2, and the lytic transglycosylase MltA, and thus reveal the form and function of the multi-enzyme complexes ..