MCM7

Summary

Gene Symbol: MCM7
Description: minichromosome maintenance complex component 7
Alias: CDC47, MCM2, P1.1-MCM3, P1CDC47, P85MCM, PNAS146, PPP1R104, DNA replication licensing factor MCM7, CDC47 homolog, homolog of S. cerevisiae Cdc47, minichromosome maintenance deficient 7, protein phosphatase 1, regulatory subunit 104
Species: human
Products:     MCM7

Top Publications

  1. Shohet J, Hicks M, Plon S, Burlingame S, Stuart S, Chen S, et al. Minichromosome maintenance protein MCM7 is a direct target of the MYCN transcription factor in neuroblastoma. Cancer Res. 2002;62:1123-8 pubmed
    ..Microarray analysis of RNA from neuroblastoma primary cell cultures revealed 10-fold higher MCM7 expression in MYCN-amplified versus nonamplified tumors...
  2. Yu Z, Feng D, Liang C. Pairwise interactions of the six human MCM protein subunits. J Mol Biol. 2004;340:1197-206 pubmed
    The eukaryotic minichromosome maintenance (MCM) proteins have six subunits, Mcm2 to 7p...
  3. Kobierzycki C, Pula B, Skiba M, Jablonska K, Latkowski K, Zabel M, et al. Comparison of minichromosome maintenance proteins (MCM-3, MCM-7) and metallothioneins (MT-I/II, MT-III) expression in relation to clinicopathological data in ovarian cancer. Anticancer Res. 2013;33:5375-83 pubmed
    ..I+II; p=0.0247). Due to the correlations shown here, the determination of MCM proteins as proliferation markers of ovarian cancer, should be strongly considered. ..
  4. Ota T, Clayton A, Minot D, Shridhar V, Hartmann L, Gilks C, et al. Minichromosome maintenance protein 7 as a potential prognostic factor for progression-free survival in high-grade serous carcinomas of the ovary. Mod Pathol. 2011;24:277-87 pubmed publisher
    Minichromosome maintenance protein 7 (MCM7) is involved in replicative licensing and synthesis of DNA...
  5. Sterner J, Dew Knight S, Musahl C, Kornbluth S, Horowitz J. Negative regulation of DNA replication by the retinoblastoma protein is mediated by its association with MCM7. Mol Cell Biol. 1998;18:2748-57 pubmed
    ..Two independent cDNAs resulting from this screen were found to encode the carboxy-terminal 137 amino acids of MCM7, a member of a family of proteins that comprise replication licensing factor...
  6. Freeman A, Morris L, Mills A, Stoeber K, Laskey R, Williams G, et al. Minichromosome maintenance proteins as biological markers of dysplasia and malignancy. Clin Cancer Res. 1999;5:2121-32 pubmed
  7. Sakwe A, Nguyen T, Athanasopoulos V, Shire K, Frappier L. Identification and characterization of a novel component of the human minichromosome maintenance complex. Mol Cell Biol. 2007;27:3044-55 pubmed
    ..MCM-BP formed a complex with MCM3 to MCM7, which excluded MCM2; and, conversely, hexameric complexes of MCM2 to MCM7 lacked MCM-BP, indicating that MCM-BP can replace MCM2 in the ..
  8. Ishimi Y, Komamura Kohno Y, You Z, Omori A, Kitagawa M. Inhibition of Mcm4,6,7 helicase activity by phosphorylation with cyclin A/Cdk2. J Biol Chem. 2000;275:16235-41 pubmed
    ..These results raise the possibility that the inactivation of Mcm4,6,7 helicase activity by Cdk2 is a part of the system for regulating DNA replication. ..
  9. Marnerides A, Vassilakopoulos T, Boltetsou E, Levidou G, Angelopoulou M, Thymara I, et al. Immunohistochemical expression and prognostic significance of CCND3, MCM2 and MCM7 in Hodgkin lymhoma. Anticancer Res. 2011;31:3585-94 pubmed
    ..Lymph node sections from 138 HL patients were immunohistochemically stained for cyclin D3 (CCND3), MCM2 and MCM7 aiming to investigate clinical outcome...

More Information

Publications98

  1. Ren B, Yu G, Tseng G, Cieply K, Gavel T, Nelson J, et al. MCM7 amplification and overexpression are associated with prostate cancer progression. Oncogene. 2006;25:1090-8 pubmed
    ..One of the identified genes, MCM7, which is a component of the DNA replication licensing complex, has been studied extensively both at the DNA and ..
  2. Liu Y, Jiang Y, Hao J, Lu S, Zhang T, Shang L, et al. Prognostic significance of MCM7 expression in the bronchial brushings of patients with non-small cell lung cancer (NSCLC). Lung Cancer. 2012;77:176-82 pubmed publisher
    ..The expression of MCM7, Ki67 and EGFR was evaluated in 494 NSCLC tissues and 174 bronchial brushings using immunohistochemical and ..
  3. Zhou Y, Zhang X, Cao L, Li B, Sui C, Li Y, et al. MCM7 expression predicts post-operative prognosis for hepatocellular carcinoma. Liver Int. 2012;32:1505-9 pubmed publisher
    Dysregulation of minichromosome maintenance protein 7 (MCM7) was previously identified in multiple human malignancies. The clinical significance of MCM7 expression is yet to be delineated in patients with hepatocellular carcinoma (HCC)...
  4. Tsao C, Geisen C, Abraham R. Interaction between human MCM7 and Rad17 proteins is required for replication checkpoint signaling. EMBO J. 2004;23:4660-9 pubmed
    ..These results demonstrate that hMCM7 plays a direct role in the transmission of DNA damage signals from active replication forks to the S-phase checkpoint machinery in human cells. ..
  5. Lau K, Chan Q, Pang J, Li K, Yeung W, Chung N, et al. Minichromosome maintenance proteins 2, 3 and 7 in medulloblastoma: overexpression and involvement in regulation of cell migration and invasion. Oncogene. 2010;29:5475-89 pubmed publisher
    ..In this study, we showed the frequent deregulation of MCM2 and MCM3 expression in 7 MB cell lines and 31 clinical samples...
  6. Toyokawa G, Masuda K, Daigo Y, Cho H, Yoshimatsu M, Takawa M, et al. Minichromosome Maintenance Protein 7 is a potential therapeutic target in human cancer and a novel prognostic marker of non-small cell lung cancer. Mol Cancer. 2011;10:65 pubmed publisher
    ..In this study, we evaluated Minichromosome Maintenance Protein 7 (MCM7) as a novel therapeutic target in cancer...
  7. Fujioka S, Shomori K, Nishihara K, Yamaga K, Nosaka K, Araki K, et al. Expression of minichromosome maintenance 7 (MCM7) in small lung adenocarcinomas (pT1): Prognostic implication. Lung Cancer. 2009;65:223-9 pubmed publisher
    ..Immunohistochemistry was conducted to obtain labeling indices (LIs%) for MCM7, MCM2 and Ki-67 in 100 surgically removed pT1 ACs...
  8. Tsuji T, Ficarro S, Jiang W. Essential role of phosphorylation of MCM2 by Cdc7/Dbf4 in the initiation of DNA replication in mammalian cells. Mol Biol Cell. 2006;17:4459-72 pubmed
    We report the identification of Cdc7/Dbf4 phosphorylation sites in human MCM2 and the determination of the role of Cdc7/Dbf4 phosphorylation of MCM2 in the initiation of DNA replication...
  9. Facoetti A, Ranza E, Grecchi I, Benericetti E, Ceroni M, Morbini P, et al. Immunohistochemical evaluation of minichromosome maintenance protein 7 in astrocytoma grading. Anticancer Res. 2006;26:3513-6 pubmed
    ..In the present study, the expressions of MCM7 and Ki67 were estimated in 66 primary human astrocytomas in relation to tumor grade (Grade I-IV, WHO)...
  10. Nishihara K, Shomori K, Fujioka S, Tokuyasu N, Inaba A, Osaki M, et al. Minichromosome maintenance protein 7 in colorectal cancer: implication of prognostic significance. Int J Oncol. 2008;33:245-51 pubmed
    ..In the present study, we evaluated the expression levels of MCM7, MCM2 and Ki67 in colorectal cancer to clarify their pathobiological significance...
  11. Cortez D, Glick G, Elledge S. Minichromosome maintenance proteins are direct targets of the ATM and ATR checkpoint kinases. Proc Natl Acad Sci U S A. 2004;101:10078-83 pubmed
    ..First, ATM phosphorylates MCM3 on S535 in response to ionizing radiation. Second, ATR phosphorylates MCM2 on S108 in response to multiple forms of DNA damage and stalling of replication forks...
  12. Johnson E, Kinoshita Y, Daniel D. A new member of the MCM protein family encoded by the human MCM8 gene, located contrapodal to GCD10 at chromosome band 20p12.3-13. Nucleic Acids Res. 2003;31:2915-25 pubmed
    The MCM8 protein from HeLa cells, a new member of the MCM family, co-isolates through several steps with MCM6 and MCM7, and MCM8 co-immunoprecipitates with MCM4, MCM6 and MCM7, proteins reportedly forming a helicase complex involved in ..
  13. Bell S, Dutta A. DNA replication in eukaryotic cells. Annu Rev Biochem. 2002;71:333-74 pubmed
    ..We emphasize recent progress in determining the function of the different replication factors once they have been assembled at the origin. ..
  14. You Z, Komamura Y, Ishimi Y. Biochemical analysis of the intrinsic Mcm4-Mcm6-mcm7 DNA helicase activity. Mol Cell Biol. 1999;19:8003-15 pubmed
    ..Recently, we reported that a DNA helicase activity is associated with an Mcm4-Mcm6-Mcm7 (Mcm4,6,7) complex, suggesting that this complex is involved in the initiation of DNA replication as a DNA-..
  15. Ishimi Y. A DNA helicase activity is associated with an MCM4, -6, and -7 protein complex. J Biol Chem. 1997;272:24508-13 pubmed
    ..Our group purified MCM protein complexes consisting of MCM2, -4 (Cdc21), -6 (Mis5), and -7 (CDC47) proteins from HeLa cells by using histone-Sepharose column chromatography (Ishimi, Y., Ichinose, S., Omori, A...
  16. Chibazakura T, Kamachi K, Ohara M, Tane S, Yoshikawa H, Roberts J. Cyclin A promotes S-phase entry via interaction with the replication licensing factor Mcm7. Mol Cell Biol. 2011;31:248-55 pubmed publisher
    ..can activate cyclin-dependent kinase but cannot promote S-phase entry, and isolated replication licensing factor Mcm7 as a factor that interacts with the wild-type cyclin A but not with the mutant...
  17. Hubbi M, Luo W, Baek J, Semenza G. MCM proteins are negative regulators of hypoxia-inducible factor 1. Mol Cell. 2011;42:700-12 pubmed publisher
    ..MCM3 inhibits transactivation domain function, whereas MCM7 enhances HIF-1? ubiquitination and proteasomal degradation...
  18. Masai H, Matsui E, You Z, Ishimi Y, Tamai K, Arai K. Human Cdc7-related kinase complex. In vitro phosphorylation of MCM by concerted actions of Cdks and Cdc7 and that of a criticial threonine residue of Cdc7 bY Cdks. J Biol Chem. 2000;275:29042-52 pubmed
    ..Among them, MCM2 protein, either in a free form or in a MCM2-4-6-7 complex, serves as an excellent substrate for huCdc7-ASK kinase ..
  19. Malaney P, Palumbo E, Semidey Hurtado J, Hardee J, Stanford K, Kathiriya J, et al. PTEN Physically Interacts with and Regulates E2F1-mediated Transcription in Lung Cancer. Cell Cycle. 2018;17:947-962 pubmed publisher
    ..Protein 1/Tripartite Motif Containing 28 MAF1 Repressor of RNA polymerase III transcription MAF1 homolog MCM2 Minichromosome Maintenance Complex Component 2 miRNA micro RNA MTF1 Metal-Regulatory Transcription Factor 1 PARP ..
  20. Haridoss S, Yovchev M, Schweizer H, Megherhi S, Beecher M, Locker J, et al. Activin A is a prominent autocrine regulator of hepatocyte growth arrest. Hepatol Commun. 2017;1:852-870 pubmed publisher
    ..genes (Ki-67, E2F transcription factor 1 [E2F1], minichromosome maintenance complex component 2 [Mcm2], forkhead box M1 [FoxM1]) and senescence-related genes (cyclin-dependent kinase inhibitor 2B [p15..
  21. Pajaud J, Ribault C, Ben Mosbah I, Rauch C, Henderson C, Bellaud P, et al. Glutathione transferases P1/P2 regulate the timing of signaling pathway activations and cell cycle progression during mouse liver regeneration. Cell Death Dis. 2015;6:e1598 pubmed publisher
    ..In addition, the expression and/or activation of cell cycle regulators such as Cyclin D1, CDK4, E2F1 and MCM7 was postponed demonstrating that the absence of GSTP1/2 delayed the entry into and progression through the G1 phase ..
  22. Naumov A, Kratzer S, Ting L, Kim K, Suvorova E, White M. The Toxoplasma Centrocone Houses Cell Cycle Regulatory Factors. MBio. 2017;8: pubmed publisher
    ..maintenance complex, and the loss of ECR1 led to increased stability of a key member of this complex, MCM2. ECR1 also forms a stable complex with the cyclin-dependent kinase (CDK)-related kinase, Tgondii Crk5 ..
  23. Mai Y, Li Y, Li R, Li W, Huang X, Mo Z, et al. Proteomic analysis of differentially expressed proteins in the marine fish parasitic ciliate Cryptocaryon irritans. Vet Parasitol. 2015;211:1-11 pubmed publisher
    ..differences in molecular weights and isoelectric points; 4 proteins (vacuolar ATP synthase catalytic subunit α, mcm2-3-5 family protein, 26S proteasome subunit P45 family protein and dnaK protein) were highly expressed only in ..
  24. Yan S, Coffing B, Li Z, Xie H, Brennick J, Beg H, et al. Diagnostic and Prognostic Value of ProEx C and GLUT1 in Melanocytic Lesions. Anticancer Res. 2016;36:2871-80 pubmed
    ..method with antibodies to topoisomerase II? (TOP2A) and minichromosome maintenance complex component 2 (MCM2) using ProEx C and to GLUT1...
  25. Choy B, LaLonde A, Que J, Wu T, Zhou Z. MCM4 and MCM7, potential novel proliferation markers, significantly correlated with Ki-67, Bmi1, and cyclin E expression in esophageal adenocarcinoma, squamous cell carcinoma, and precancerous lesions. Hum Pathol. 2016;57:126-135 pubmed publisher
    ..Our study investigated the correlation between MCM4 and MCM7 expression and Ki-67, Bmi1, and cyclin E expression in esophageal adenocarcinoma, squamous cell carcinoma, and ..
  26. Li S, Xue W, Khoo U, Ngan H, Chan K, Tam I, et al. Replicative MCM7 protein as a proliferation marker in endometrial carcinoma: a tissue microarray and clinicopathological analysis. Histopathology. 2005;46:307-13 pubmed
    ..the proliferative activity of endometrial carcinoma using one of the minichromosome maintenance (MCM) proteins (MCM7), and to explore its potential value for prognosis...
  27. Ortega M, Marh J, Alarcon V, Ward W. Unique pattern of ORC2 and MCM7 localization during DNA replication licensing in the mouse zygote. Biol Reprod. 2012;87:62 pubmed publisher
    ..We examined the subcellular localization of two licensing proteins, ORC2 and MCM7, in the mouse zygotes and two-cell embryos...
  28. Reinapae A, Jalakas K, Avvakumov N, Lõoke M, Kristjuhan K, Kristjuhan A. Recruitment of Fkh1 to replication origins requires precisely positioned Fkh1/2 binding sites and concurrent assembly of the pre-replicative complex. PLoS Genet. 2017;13:e1006588 pubmed publisher
    ..recruitment of Fkh1 to the origins depends on formation of the pre-replicative complex (pre-RC) and loading of the Mcm2-7 helicase, indicating that the origins are regulated by cooperative action of Fkh1 and the pre-RC...
  29. Fristrup N, Birkenkamp Demtroder K, Reinert T, Sanchez Carbayo M, Segersten U, Malmstrom P, et al. Multicenter validation of cyclin D1, MCM7, TRIM29, and UBE2C as prognostic protein markers in non-muscle-invasive bladder cancer. Am J Pathol. 2013;182:339-49 pubmed publisher
    Transcripts from the four genes encoding cyclin D1, MCM7, TRIM29, and UBE2C have previously been included in gene expression signatures for outcome prediction in stage Ta/T1 urothelial carcinomas...
  30. Pruitt S, Qin M, Wang J, Kunnev D, Freeland A. A Signature of Genomic Instability Resulting from Deficient Replication Licensing. PLoS Genet. 2017;13:e1006547 pubmed publisher
    ..In MCM2 deficient mice a different set of preferred breakage sites is identified that includes the tumor suppressor gene ..
  31. Boskovic J, Coloma J, Aparicio T, Zhou M, Robinson C, Mendez J, et al. Molecular architecture of the human GINS complex. EMBO Rep. 2007;8:678-84 pubmed
    ..with cell-division cycle protein (Cdc)45 and the minichromosome maintenance mutant (MCM)2-7 to form the Cdc45/Mcm2-7/GINS (CMG) complex, the presumed replicative helicase...
  32. Chen Z, Yu Y, Michalopoulos G, Nelson J, Luo J. The DNA replication licensing factor miniature chromosome maintenance 7 is essential for RNA splicing of epidermal growth factor receptor, c-Met, and platelet-derived growth factor receptor. J Biol Chem. 2015;290:1404-11 pubmed publisher
    Miniature chromosome maintenance 7 (MCM7) is an essential component of DNA replication licensing complex. Recent studies indicate that MCM7 is amplified and overexpressed in a variety of human malignancies...
  33. Vedam Mai V, Baradaran Shoraka M, Reynolds B, Okun M. Tissue Response to Deep Brain Stimulation and Microlesion: A Comparative Study. Neuromodulation. 2016;19:451-8 pubmed publisher
    ..0013, mcm2; p?=?0.0002)...
  34. Samel S, Fernández Cid A, Sun J, Riera A, Tognetti S, Herrera M, et al. A unique DNA entry gate serves for regulated loading of the eukaryotic replicative helicase MCM2-7 onto DNA. Genes Dev. 2014;28:1653-66 pubmed publisher
    The regulated loading of the replicative helicase minichromosome maintenance proteins 2-7 (MCM2-7) onto replication origins is a prerequisite for replication fork establishment and genomic stability...
  35. Li W, Cao F, Li J, Wang Z, Ren Y, Liang Z, et al. Simvastatin exerts anti-hepatitis B virus activity by inhibiting expression of minichromosome maintenance protein 7 in HepG2.2.15 cells. Mol Med Rep. 2016;14:5334-5342 pubmed publisher
    ..The present study demonstrated that downregulation of MCM7 inhibited the expression of proteins transferred by adenoviral vectors...
  36. Georgescu R, Yuan Z, Bai L, de Luna Almeida Santos R, Sun J, Zhang D, et al. Structure of eukaryotic CMG helicase at a replication fork and implications to replisome architecture and origin initiation. Proc Natl Acad Sci U S A. 2017;114:E697-E706 pubmed publisher
    The eukaryotic CMG (Cdc45, Mcm2-7, GINS) helicase consists of the Mcm2-7 hexameric ring along with five accessory factors...
  37. Zhai Y, Tye B. Structure of the MCM2-7 Double Hexamer and Its Implications for the Mechanistic Functions of the Mcm2-7 Complex. Adv Exp Med Biol. 2017;1042:189-205 pubmed publisher
    ..8 Å. This review will focus on the fine details observed in the Mcm2-7 double hexameric complex and their implications for the function of the Mcm2-7 hexamer in its different roles ..
  38. Gao J, Wang Q, Dong C, Chen S, Qi Y, Liu Y. Whole Exome Sequencing Identified MCM2 as a Novel Causative Gene for Autosomal Dominant Nonsyndromic Deafness in a Chinese Family. PLoS ONE. 2015;10:e0133522 pubmed publisher
    ..Using whole exome sequencing, we identified a missense variant (c.130C>T, p.R44C) in the MCM2 gene, which has a pro-apoptosis effect and is involved in the initiation of eukaryotic genome replication...
  39. Raja H, El Elimat T, Oberlies N, Shearer C, Miller A, Tanaka K, et al. Minutisphaerales (Dothideomycetes, Ascomycota): a new order of freshwater ascomycetes including a new family, Minutisphaeraceae, and two new species from North Carolina, USA. Mycologia. 2015;107:845-62 pubmed publisher
    ..8SITS2 (ITS) and 28S nuc rDNA, and a protein-coding gene, MCM7, for newly collected strains of Minutisphaera...
  40. Douglas M, Diffley J. Recruitment of Mcm10 to Sites of Replication Initiation Requires Direct Binding to the Minichromosome Maintenance (MCM) Complex. J Biol Chem. 2016;291:5879-88 pubmed publisher
    ..Moreover, the binding site for Mcm10 on MCM includes the Mcm2 and Mcm6 subunits and overlaps that for the loading factor Cdt1...
  41. Wang Y, Wang Y, Duan X, Wang Y, Zhang Z. Interleukin-1 receptor-associated kinase 1 correlates with metastasis and invasion in endometrial carcinoma. J Cell Biochem. 2017;: pubmed publisher
    ..The expression of mitotic cell cycle (CDK1 and Cdc45) and cell division pathway (Cdc7 and MCM2) related factors was significantly suppressed by IRAK1 knockdown...
  42. Campolina Silva G, Hess R, Oliveira C. Seasonal variation of cell proliferation and apoptosis in the efferent ductules and epididymis of the Neotropical bat Artibeus lituratus (Chiroptera, Phyllostomidae). Gen Comp Endocrinol. 2019;273:3-10 pubmed publisher
    ..lituratus during the reproductive period, as few epithelial cells were positive for MCM7 (proliferation marker) and cleaved caspase-3 or TUNEL (apoptosis markers)...
  43. Feng C, Li H, Li J, Lu Y, Liao G. Expression of Mcm7 and Cdc6 in oral squamous cell carcinoma and precancerous lesions. Anticancer Res. 2008;28:3763-9 pubmed
    The present study aimed at evaluating the clinical importance of Mcm7 and Cdc6 expression in oral squamous cell carcinoma (OSCC) and precancerous lesions.
  44. Xu X, Rochette P, Feyissa E, Su T, Liu Y. MCM10 mediates RECQ4 association with MCM2-7 helicase complex during DNA replication. EMBO J. 2009;28:3005-14 pubmed publisher
    ..We found that essential replisome factors MCM10, MCM2-7 helicase, CDC45 and GINS are the primary interaction partner proteins of human RECQ4...
  45. Diffley J. The many faces of redundancy in DNA replication control. Cold Spring Harb Symp Quant Biol. 2010;75:135-42 pubmed publisher
    ..This is achieved by a two-step mechanism. The first step, licensing, involves the loading of the Mcm2-7 proteins into pre-replicative complexes (pre-RCs) at origins by ORC, Cdc6, and Cdt1...
  46. Huang H, Strømme C, Saredi G, Hödl M, Strandsby A, González Aguilera C, et al. A unique binding mode enables MCM2 to chaperone histones H3-H4 at replication forks. Nat Struct Mol Biol. 2015;22:618-26 pubmed publisher
    ..Here, we reveal how human MCM2, part of the replicative helicase, chaperones histones H3-H4...
  47. Dewar J, Low E, Mann M, Raschle M, Walter J. CRL2Lrr1 promotes unloading of the vertebrate replisome from chromatin during replication termination. Genes Dev. 2017;31:275-290 pubmed publisher
    ..CMG unloading involves ubiquitylation of its Mcm7 subunit and the action of the p97 ATPase...
  48. Kliszczak M, Sedlackova H, Pitchai G, Streicher W, Krejci L, Hickson I. Interaction of RECQ4 and MCM10 is important for efficient DNA replication origin firing in human cells. Oncotarget. 2015;6:40464-79 pubmed publisher
    ..These origins are bound by the origin recognition complex (ORC), which subsequently recruits the Mcm2-7 replicative helicase in a Cdt1/Cdc6-dependent manner...
  49. Buchsbaum S, Morris C, Bochard V, Jalinot P. Human INT6 interacts with MCM7 and regulates its stability during S phase of the cell cycle. Oncogene. 2007;26:5132-44 pubmed
    ..In this report, we investigated the functional significance of the interaction between INT6 and MCM7, which was observed in a two-hybrid screen performed with INT6 as bait...
  50. Ishimi Y, Irie D. G364R mutation of MCM4 detected in human skin cancer cells affects DNA helicase activity of MCM4/6/7 complex. J Biochem. 2015;157:561-9 pubmed publisher
    ..These results suggest that this mutation does not affect assembly of MCM2-7 complex on replication origins but it interferes some step at function of MCM2-7 helicase...
  51. Rojiani M, Siemann D, Rojiani A. Cell proliferation index determination by immunohistochemical detection of hCDC47 protein. Appl Immunohistochem Mol Morphol. 2010;18:278-82 pubmed publisher
    A member of the human minichromosome maintenance complex protein family, hCDC47 (alias MCM7) has been identified as a component of the regulatory mechanism in cell proliferation...
  52. Dumit V, Kuttner V, Käppler J, Piera Velazquez S, Jimenez S, Bruckner Tuderman L, et al. Altered MCM protein levels and autophagic flux in aged and systemic sclerosis dermal fibroblasts. J Invest Dermatol. 2014;134:2321-2330 pubmed publisher
    ..We observed that lower levels of MCM7 correlate with reduced cell proliferation, lower autophagic capacity, and higher intracellular protein abundance ..
  53. Kang S, Kang M, Ryu E, Myung K. Eukaryotic DNA replication: Orchestrated action of multi-subunit protein complexes. Mutat Res. 2018;809:58-69 pubmed publisher
    ..One of the key replicative proteins that participate at every stage of DNA replication is the Mcm2-7 complex, a replicative helicase...
  54. Yankulov K, Todorov I, Romanowski P, Licatalosi D, Cilli K, McCracken S, et al. MCM proteins are associated with RNA polymerase II holoenzyme. Mol Cell Biol. 1999;19:6154-63 pubmed
    ..We report that antibodies against MCM2(BM28) specifically inhibited transcription by RNA polymerase II (Pol II) in microinjected Xenopus oocytes...
  55. Wang Q, Li Q, Liu R, Zheng M, Wen J, Zhao G. Host cell interactome of PA protein of H5N1 influenza A virus in chicken cells. J Proteomics. 2016;136:48-54 pubmed publisher
    ..strong enrichment of PA interacting proteins in the category of DNA replication, covering genes such as PCNA, MCM2, MCM3, MCM4, MCM5 and MCM7...
  56. Liu Y, Wang B, Jiang Y, Cao J, Hao J, Zhang Y, et al. MCMs expression in lung cancer: implication of prognostic significance. J Cancer. 2017;8:3641-3647 pubmed publisher
    ..We evaluated the expression of MCM2, MCM5 and MCM6 immunohistochemically in 571 primary NSCLC samples...
  57. Yoshida K, Inoue I. Conditional expression of MCM7 increases tumor growth without altering DNA replication activity. FEBS Lett. 2003;553:213-7 pubmed
    ..Here, we generated a cell line MCM7(+/-)/MCM7-FLAG, in which one allele of MCM7 is mutated whereas a tetracycline-repressible promoter could manipulate ..
  58. Winther T, Torp S. MCM7 expression is a promising predictor of recurrence in patients surgically resected for meningiomas. J Neurooncol. 2017;131:575-583 pubmed publisher
    ..the prediction of tumor recurrence, this study evaluated and compared the prognostic usefulness of the biomarker MCM7 with the conventional mitotic index and the MIB-1 biomarker...
  59. Cheung C, Hsu C, Chen K, Chong S, Wu C, Huang H, et al. MCM2-regulated functional networks in lung cancer by multi-dimensional proteomic approach. Sci Rep. 2017;7:13302 pubmed publisher
    ..Malignancies often exhibit defective minichromosome maintenance protein 2 (MCM2), a cancer proliferation biomarker that serves as a licensing factor in the initiation of DNA replication...
  60. Yang X, Qu K, Tao J, Yin G, Han S, Liu Q, et al. Inhibition of CIP2A attenuates tumor progression by inducing cell cycle arrest and promoting cellular senescence in hepatocellular carcinoma. Biochem Biophys Res Commun. 2018;495:1807-1814 pubmed publisher
    ..with CIP2A knockdown, companying expression changes of senescence associated proteins (p21, CDK2, CDK4, cyclin D1, MCM7 and FoxM1). Mechanistically, CIP2A knockdown repressed FoxM1 expression and induced FoxM1 dephosphorylation...
  61. Todorov I, Lavigne J, Sakr F, Kaneva R, Foisy S, Bibor Hardy V. Nuclear matrix protein mitotin messenger RNA is expressed at constant levels during the cell cycle. Biochem Biophys Res Commun. 1991;177:395-400 pubmed
    ..In this report, using synchronized Raji and WISH cells, we demonstrate that mitotin messenger RNA is expressed at the same level throughout the cell cycle. ..
  62. Sugimoto N, Kitabayashi I, Osano S, Tatsumi Y, Yugawa T, Narisawa Saito M, et al. Identification of novel human Cdt1-binding proteins by a proteomics approach: proteolytic regulation by APC/CCdh1. Mol Biol Cell. 2008;19:1007-21 pubmed
    ..Thus, in addition to SCF(Skp2) and cullin4-based ubiquitin ligases, APC/C(Cdh1) is a third ubiquitin ligase that plays a crucial role in proteolytic regulation of Cdt1 in mammalian cells. ..
  63. Kinoshita Y, Johnson E, Gordon R, Negri Bell H, Evans M, Coolbaugh J, et al. Colocalization of MCM8 and MCM7 with proteins involved in distinct aspects of DNA replication. Microsc Res Tech. 2008;71:288-97 pubmed
    Minichromosome maintenance (MCM) proteins are essential for DNA replication in eukaryotes. A subcomplex of the MCM2-7 family members, initially characterized in yeast, is thought to serve as a eukaryotic DNA replicative helicase...
  64. Choi J, Lim H, Kim J, Song O, Cho Y. Crystal structure of the human GINS complex. Genes Dev. 2007;21:1316-21 pubmed
    The GINS complex mediates the assembly of the MCM2-7 (minichromosome maintenance) complex with proteins in a replisome progression complex...
  65. Ask K, Jasencakova Z, Menard P, Feng Y, Almouzni G, Groth A. Codanin-1, mutated in the anaemic disease CDAI, regulates Asf1 function in S-phase histone supply. EMBO J. 2012;31:2013-23 pubmed publisher
    ..This function is compromised by two CDAI mutations that impair complex formation with Asf1, providing insight into the molecular basis for CDAI disease. ..
  66. Zheng L, Kanagaraj R, Mihaljevic B, Schwendener S, Sartori A, Gerrits B, et al. MRE11 complex links RECQ5 helicase to sites of DNA damage. Nucleic Acids Res. 2009;37:2645-57 pubmed publisher
    ..Collectively, these data suggest that the MRN complex recruits RECQ5 to sites of DNA damage to regulate DNA repair. ..
  67. Zhang X, Tang L, Ren B, Yu Y, Nelson J, Michalopoulos G, et al. Interaction of MCM7 and RACK1 for activation of MCM7 and cell growth. Am J Pathol. 2013;182:796-805 pubmed publisher
    b>MCM7 is one of the pivotal DNA replication licensing factors in controlling DNA synthesis and cell entry into S phase...
  68. Brake T, Connor J, Petereit D, Lambert P. Comparative analysis of cervical cancer in women and in a human papillomavirus-transgenic mouse model: identification of minichromosome maintenance protein 7 as an informative biomarker for human cervical cancer. Cancer Res. 2003;63:8173-80 pubmed
    ..We then used our mouse model to identify minichromosome maintenance protein 7 (MCM7), an E2F-induced cellular DNA replication factor, as a novel biomarker for cervical cancer...
  69. Tian L, Liu J, Xia G, Chen B. RNAi-mediated knockdown of MCM7 gene on CML cells and its therapeutic potential for leukemia. Med Oncol. 2017;34:21 pubmed publisher
    b>MCM7 is one of the subunits of MCM2-7 complex, which is essential to DNA replication licensing and the control of cell cycle progression...
  70. Bruemmer D, Yin F, Liu J, Kiyono T, Fleck E, Van Herle A, et al. Expression of minichromosome maintenance proteins in vascular smooth muscle cells is ERK/MAPK dependent. Exp Cell Res. 2003;290:28-37 pubmed
    ..studies were designed to investigate the signal transduction pathways controlling the expression of MCM6 and MCM7 in VSMC in response to mitogenic stimuli...
  71. Jankowska Konsur A, Kobierzycki C, Reich A, Grzegrzolka J, Maj J, Dziegiel P. Expression of MCM-3 and MCM-7 in Primary Cutaneous T-cell Lymphomas. Anticancer Res. 2015;35:6017-26 pubmed
    ..Further studies are required to analyze their implementation into patient stratification and treatment process such that will improve prognosis in CTCL. ..
  72. Wang H, Li Y, Guo H, Yu L, Wu Z, Yin J, et al. A cocktail of p16INK4a and Ki-67, p16INK4a and minichromosome maintenance protein 2 as triage tests for human papillomavirus primary cervical cancer screening. Oncotarget. 2017;8:83890-83899 pubmed publisher
    ..We evaluated the accuracy of p16/Ki-67 and p16/mcm2 dual staining as biomarkers for triaging HPV positive women in China...
  73. Schaarschmidt D, Ladenburger E, Keller C, Knippers R. Human Mcm proteins at a replication origin during the G1 to S phase transition. Nucleic Acids Res. 2002;30:4176-85 pubmed
    ..Thus, human Mcm proteins first assemble at or adjacent to bound ORC and move to other sites during genome replication. ..
  74. Petrak J, Toman O, Simonova T, Halada P, Cmejla R, Klener P, et al. Identification of molecular targets for selective elimination of TRAIL-resistant leukemia cells. From spots to in vitro assays using TOP15 charts. Proteomics. 2009;9:5006-15 pubmed publisher
    ..Decreased expression of DNA replication and maintenance proteins MCM7 and RPA32 in HL-60/P1 cells, and the marked down-regulation of enzyme adenosine deaminase in HL-60/P2 cells, ..
  75. Hua C, Zhao G, Li Y, Bie L. Minichromosome Maintenance (MCM) Family as potential diagnostic and prognostic tumor markers for human gliomas. BMC Cancer. 2014;14:526 pubmed publisher
    ..We found that MCMs expression was significantly up-regulated in glioma samples. MCM2-7 and MCM10 expressions were associated with WHO tumor grade...
  76. You Z, Masai H. Potent DNA strand annealing activity associated with mouse Mcm2?7 heterohexameric complex. Nucleic Acids Res. 2017;45:6494-6506 pubmed publisher
    ..b>Mcm2?7, each containing a conserved ATPase motif, form a six subunit-heterohexamer...
  77. Saade E, Mechold U, Kulyyassov A, Vertut D, Lipinski M, Ogryzko V. Analysis of interaction partners of H4 histone by a new proteomics approach. Proteomics. 2009;9:4934-43 pubmed publisher
    ..In addition, we report all components of the licensing MCM2-7 complex and the apoptosis-related DAXX protein among the interaction partners of the soluble H4...
  78. Mlcochova P, Caswell S, Taylor I, Towers G, Gupta R. DNA damage induced by topoisomerase inhibitors activates SAMHD1 and blocks HIV-1 infection of macrophages. EMBO J. 2018;37:50-62 pubmed publisher
    ..We report that SAMHD1 was activated by dephosphorylation following ETO treatment, along with loss of expression of MCM2 and CDK1, and reduction in dNTP levels...
  79. Chan K, Tsui S, Ngai S, Lee S, Kotaka M, Waye M, et al. Protein-protein interaction of FHL2, a LIM domain protein preferentially expressed in human heart, with hCDC47. J Cell Biochem. 2000;76:499-508 pubmed
  80. Hernández Pérez S, Cabrera E, Amoedo H, Rodriguez Acebes S, Koundrioukoff S, Debatisse M, et al. USP37 deubiquitinates Cdt1 and contributes to regulate DNA replication. Mol Oncol. 2016;10:1196-206 pubmed publisher
    ..origin of replication, and the Cdt1 and Cdc6 proteins, that license these origins to replicate by recruiting the MCM2-7 helicase. To accurately achieve its functions, Cdt1 is tightly regulated...
  81. DaFonseca C, Shu F, Zhang J. Identification of two residues in MCM5 critical for the assembly of MCM complexes and Stat1-mediated transcription activation in response to IFN-gamma. Proc Natl Acad Sci U S A. 2001;98:3034-9 pubmed
    ..Together, these results strongly suggest that Stat1 recruits a MCM5/3 subcomplex through direct interaction with MCM5 in the process of IFN-gamma-induced gene activation. ..
  82. Kusunoki S, Ishimi Y. Interaction of human minichromosome maintenance protein-binding protein with minichromosome maintenance 2-7. FEBS J. 2014;281:1057-67 pubmed publisher
    ..The results show that MCM-BP mainly interacts with MCM7 in the Triton-soluble fraction from S phase and G(2) phase cells, and it also interacts with structural maintenance ..
  83. Lossaint G, Larroque M, Ribeyre C, Bec N, Larroque C, Décaillet C, et al. FANCD2 binds MCM proteins and controls replisome function upon activation of s phase checkpoint signaling. Mol Cell. 2013;51:678-90 pubmed publisher
    ..ATR signaling promoted the transient association of endogenous FANCD2 with the MCM2-MCM7 replicative helicase independently of FANCD2 monoubiquitination...
  84. Riera A, Fernández Cid A, Speck C. The ORC/Cdc6/MCM2–7 complex, a new power player for regulated helicase loading. Cell Cycle. 2013;12:2155-6 pubmed
  85. Zhong H, Chen B, Neves H, Xing J, Ye Y, Lin Y, et al. Expression of minichromosome maintenance genes in renal cell carcinoma. Cancer Manag Res. 2017;9:637-647 pubmed publisher
    ..We found that the expression levels of MCM genes are positively correlated with one another. Expression levels of MCM2, MCM5, MCM6, and MCM7, but not of MCM3 and MCM4, were higher in RCC compared to paired adjacent normal tissue...
  86. Sar F, Lindsey Boltz L, Subramanian D, Croteau D, Hutsell S, Griffith J, et al. Human claspin is a ring-shaped DNA-binding protein with high affinity to branched DNA structures. J Biol Chem. 2004;279:39289-95 pubmed
  87. Maric M, Mukherjee P, Tatham M, Hay R, Labib K. Ufd1-Npl4 Recruit Cdc48 for Disassembly of Ubiquitylated CMG Helicase at the End of Chromosome Replication. Cell Rep. 2017;18:3033-3042 pubmed publisher
    ..is the key regulated step during DNA replication termination in eukaryotes, involving ubiquitylation of the Mcm7 helicase subunit, leading to a disassembly process that requires the Cdc48 "segregase"...
  88. Kim D, Kim J, Moon J, Kim K, Kim T, Hong S, et al. Transcriptional induction of minichromosome maintenance protein 7 (Mcm7) in human cholangiocarcinoma cells treated with Clonorchis sinensis excretory-secretory products. Mol Biochem Parasitol. 2010;173:10-6 pubmed publisher
    ..Among the upregulated genes, we focused on minichromosome maintenance protein 7 (Mcm7), which is implicated in various cancer types, and analyzed transcriptional regulation mediated by ESP to further ..
  89. Musahl C, Schulte D, Burkhart R, Knippers R. A human homologue of the yeast replication protein Cdc21. Interactions with other Mcm proteins. Eur J Biochem. 1995;230:1096-101 pubmed
    ..Protein hCdc21 forms a stable trimeric complex with two novel human Mcm proteins, p85Mcm and p105Mcm. Protein BM28/Mcm2 is more loosely associated with the trimeric hCdc21 complex.