Gene Symbol: MCM4
Description: minichromosome maintenance complex component 4
Alias: CDC21, CDC54, IMD54, NKCD, NKGCD, P1-CDC21, hCdc21, DNA replication licensing factor MCM4, CDC21 homolog, homolog of S. pombe cell devision cycle 21, minichromosome maintenance deficient 4
Species: human
Products:     MCM4

Top Publications

  1. Ishimi Y, Komamura Kohno Y, You Z, Omori A, Kitagawa M. Inhibition of Mcm4,6,7 helicase activity by phosphorylation with cyclin A/Cdk2. J Biol Chem. 2000;275:16235-41 pubmed
    ..Our group has reported that a 600-kDa Mcm4,6,7 complex has a DNA helicase activity that is probably necessary for the initiation of DNA replication...
  2. Ishimi Y, Komamura Kohno Y. Phosphorylation of Mcm4 at specific sites by cyclin-dependent kinase leads to loss of Mcm4,6,7 helicase activity. J Biol Chem. 2001;276:34428-33 pubmed vivo, and cyclin-dependent protein kinase is at least in part responsible for the phosphorylation of Mcm4. Our group reported that the DNA helicase activity of Mcm4,6,7 complex, which may be involved in initiation of DNA ..
  3. Tsuji T, Ficarro S, Jiang W. Essential role of phosphorylation of MCM2 by Cdc7/Dbf4 in the initiation of DNA replication in mammalian cells. Mol Biol Cell. 2006;17:4459-72 pubmed
    ..Taken together, these results indicate that Cdc7/Dbf4 phosphorylation of MCM2 is essential for the initiation of DNA replication in mammalian cells. ..
  4. Masai H, Taniyama C, Ogino K, Matsui E, Kakusho N, Matsumoto S, et al. Phosphorylation of MCM4 by Cdc7 kinase facilitates its interaction with Cdc45 on the chromatin. J Biol Chem. 2006;281:39249-61 pubmed
    ..We have analyzed phosphorylation of MCM subunits during cell cycle by examining mobility shift on SDS-PAGE. MCM4 on the chromatin undergoes specific phosphorylation during S phase...
  5. Ishimi Y. A DNA helicase activity is associated with an MCM4, -6, and -7 protein complex. J Biol Chem. 1997;272:24508-13 pubmed
    ..Our group purified MCM protein complexes consisting of MCM2, -4 (Cdc21), -6 (Mis5), and -7 (CDC47) proteins from HeLa cells by using histone-Sepharose column chromatography (Ishimi, Y...
  6. Bell S, Dutta A. DNA replication in eukaryotic cells. Annu Rev Biochem. 2002;71:333-74 pubmed
    ..We emphasize recent progress in determining the function of the different replication factors once they have been assembled at the origin. ..
  7. Yu Z, Feng D, Liang C. Pairwise interactions of the six human MCM protein subunits. J Mol Biol. 2004;340:1197-206 pubmed
    ..Thus, this study may serve as a foundation for understanding the overall architecture and function of eukaryotic MCM protein complexes and as clues for developing anticancer drugs targeted to the human MCM proteins. ..
  8. Sakwe A, Nguyen T, Athanasopoulos V, Shire K, Frappier L. Identification and characterization of a novel component of the human minichromosome maintenance complex. Mol Cell Biol. 2007;27:3044-55 pubmed
    ..MCM-BP also formed a complex with the MCM4/6/7 core helicase in vitro, but, unlike MCM2, did not inhibit this helicase activity...
  9. Kikuchi J, Kinoshita I, Shimizu Y, Kikuchi E, Takeda K, Aburatani H, et al. Minichromosome maintenance (MCM) protein 4 as a marker for proliferation and its clinical and clinicopathological significance in non-small cell lung cancer. Lung Cancer. 2011;72:229-37 pubmed publisher
    ..of non-small cell lung cancer (NSCLC) cells by a cJun dominant-negative mutant, we found that reduced expression of MCM4 was correlated with this growth suppression...

More Information

Publications126 found, 100 shown here

  1. Fujita M, Kiyono T, Hayashi Y, Ishibashi M. In vivo interaction of human MCM heterohexameric complexes with chromatin. Possible involvement of ATP. J Biol Chem. 1997;272:10928-35 pubmed
    ..Nuclease digestion-immunoprecipitation techniques of the type described here should facilitate further elucidation of the mode of interaction between hMCMs and chromatin. ..
  2. Masai H, Matsui E, You Z, Ishimi Y, Tamai K, Arai K. Human Cdc7-related kinase complex. In vitro phosphorylation of MCM by concerted actions of Cdks and Cdc7 and that of a criticial threonine residue of Cdc7 bY Cdks. J Biol Chem. 2000;275:29042-52 pubmed
    ..b>MCM4 and MCM6 are also phosphorylated by huCdc7 albeit to less extent...
  3. Rizvi I, Choudhury N, Tuteja N. Arabidopsis thaliana MCM3 single subunit of MCM2-7 complex functions as 3' to 5' DNA helicase. Protoplasma. 2016;253:467-75 pubmed publisher
    ..The MCM2-7 and MCM4/6/7subcomplex exhibit DNA helicase activity, and are therefore regarded as the replicative helicase...
  4. Fernández Rubio C, Larrea E, Peña Guerrero J, Sesma Herrero E, Gamboa I, Berrio C, et al. Leishmanicidal Activity of Isoselenocyanate Derivatives. Antimicrob Agents Chemother. 2019;63: pubmed publisher
    ..genes involved in the cell cycle, such as topoisomerase-2 (TOP-2), PCNA, and MCM4, therefore contributing to its leishmanicidal activity...
  5. McNairn A, Chuang C, Bloom J, Wallace M, Schimenti J. Female-biased embryonic death from inflammation induced by genomic instability. Nature. 2019;567:105-108 pubmed publisher
    ..In addition, deficiency in the anti-inflammatory IL10 receptor was synthetically lethal with the Mcm4Chaos3 helicase mutant...
  6. Guo W, Sarkar S, Peddada S. Controlling false discoveries in multidimensional directional decisions, with applications to gene expression data on ordered categories. Biometrics. 2010;66:485-92 pubmed publisher
    ..We identified several important cell-cycle genes, such as DNA replication/repair gene MCM4 and replication factor subunit C2, which were not identified by the previous analyses of the same data by ..
  7. Liao X, Han C, Wang X, Huang K, Yu T, Yang C, et al. Prognostic value of minichromosome maintenance mRNA expression in early-stage pancreatic ductal adenocarcinoma patients after pancreaticoduodenectomy. Cancer Manag Res. 2018;10:3255-3271 pubmed publisher
    ..Through mining public databases, we observed that MCM genes (MCM2, MCM3, MCM4, MCM5, MCM6, and MCM7) were upregulated in pancreatic cancer tumor tissue and have a ..
  8. Froelich C, Nourse A, Enemark E. MCM ring hexamerization is a prerequisite for DNA-binding. Nucleic Acids Res. 2015;43:9553-63 pubmed publisher
    ..that exhibits severely defective hexamerization and ssDNA-binding is at a conserved phenylalanine that aligns with the mouse Mcm4(Chaos3) mutation associated with chromosomal instability, cancer, and decreased intersubunit association.
  9. Ishimi Y. Regulation of MCM2-7 function. Genes Genet Syst. 2018;93:125-133 pubmed publisher
    ..A point mutation in MCM4 that disturbs MCM2-7 function results in genomic instability, leading to the generation of cancer cells...
  10. von Eyss B, Maaskola J, Memczak S, Möllmann K, Schuetz A, Loddenkemper C, et al. The SNF2-like helicase HELLS mediates E2F3-dependent transcription and cellular transformation. EMBO J. 2012;31:972-85 pubmed publisher
    ..Our work reveals that HELLS is important for E2F3 in tumour cell proliferation. ..
  11. Li T, Luo W, Liu K, Lv X, Xi T. miR-31 promotes proliferation of colon cancer cells by targeting E2F2. Biotechnol Lett. 2015;37:523-32 pubmed publisher
    ..suppressor in colon cancer by repressing the expression of survivin and regulating the expression of CCNA2, C-MYC, MCM4 and CDK2...
  12. Cohen J. Primary Immunodeficiencies Associated with EBV Disease. Curr Top Microbiol Immunol. 2015;390:241-65 pubmed publisher
    ..These include diseases due to mutations in PIK3CD, PIK3R1, CTPS1, STK4, GATA2, MCM4, FCGR3A, CARD11, ATM, and WAS...
  13. Bruck I, Pérez Arnaiz P, Colbert M, Kaplan D. Insights into the Initiation of Eukaryotic DNA Replication. Nucleus. 2015;6:449-54 pubmed publisher
    ..DDK, on the other hand, phosphorylates the Mcm2, Mcm4, and Mcm6 subunits of the Mcm2-7 complex...
  14. Sorte H, Osnes L, Fevang B, Aukrust P, Erichsen H, Backe P, et al. A potential founder variant in CARMIL2/RLTPR in three Norwegian families with warts, molluscum contagiosum, and T-cell dysfunction. Mol Genet Genomic Med. 2016;4:604-616 pubmed
    ..and a differential molecular diagnosis to CXCR4-,DOCK8-,GATA2-,MAGT1-,MCM4-,STK4-,RHOH-,TMC6-, and TMC8-related diseases...
  15. Toteva T, Mason B, Kanoh Y, Brøgger P, Green D, Verhein Hansen J, et al. Establishment of expression-state boundaries by Rif1 and Taz1 in fission yeast. Proc Natl Acad Sci U S A. 2017;114:1093-1098 pubmed publisher
    ..Moreover, efficient boundary formation required the amino-terminal domain of the Mcm4 replicative helicase onto which the antagonistic activities of the replication-promoting Dbf4-dependent kinase and ..
  16. Dowling J, Becker C, Bourke N, Corr S, Connolly D, Quinn S, et al. Promyelocytic leukemia protein interacts with the apoptosis-associated speck-like protein to limit inflammasome activation. J Biol Chem. 2014;289:6429-37 pubmed publisher
    ..Collectively, our data indicate that PML limits ASC function, retaining ASC in the nucleus. ..
  17. Terrell A, Anand D, Smith S, Dang C, Waters S, Pathania M, et al. Molecular characterization of mouse lens epithelial cell lines and their suitability to study RNA granules and cataract associated genes. Exp Eye Res. 2015;131:42-55 pubmed publisher
    ..Expression of lens epithelium-enriched genes Foxe3, Pax6, Anxa4 and Mcm4 is up-regulated in LEC lines, compared to isolated lens fiber cells...
  18. Dong L, Li H, Zhang S, Su L. Identification of genes related to consecutive trauma-induced sepsis via gene expression profiling analysis. Medicine (Baltimore). 2018;97:e0362 pubmed publisher
    ..Some genes composing protein complex A were associated with cell proliferation (eg, CDC20, CCNB1, MCM4, RPA2, and PRIM2), and several genes composing protein complex F were implicated in regulation of actin ..
  19. Lee Y, Park S, Lee S, Lee H. Characterization of genetic aberrations in a single case of metastatic thymic adenocarcinoma. BMC Cancer. 2017;17:330 pubmed publisher
    ..number analysis revealed a complex chromosomal rearrangement of chromosome 8, which resulted in gene fusion between MCM4 and SNTB1 and dramatic amplification of MYC and NDRG1...
  20. Leturcq M, Mortuaire M, Hardiville S, Schulz C, Lefebvre T, Vercoutter Edouart A. O-GlcNAc transferase associates with the MCM2-7 complex and its silencing destabilizes MCM-MCM interactions. Cell Mol Life Sci. 2018;75:4321-4339 pubmed publisher
    ..Finally, OGT silencing or OGA inhibition destabilizes MCM2/6 and MCM4/7 interactions in the chromatin-enriched fraction...
  21. Kucherlapati M. Examining transcriptional changes to DNA replication and repair factors over uveal melanoma subtypes. BMC Cancer. 2018;18:818 pubmed publisher
    ..Genes with significant alteration include MCM2, MCM4, MCM5, CDC45, MCM10, CIZ1, PCNA, FEN1, LIG1, POLD1, POLE, HUS1, CHECK1, ATRIP, MLH3, and MSH6...
  22. Wang Q, Li Q, Liu R, Zheng M, Wen J, Zhao G. Host cell interactome of PA protein of H5N1 influenza A virus in chicken cells. J Proteomics. 2016;136:48-54 pubmed publisher
    ..enrichment of PA interacting proteins in the category of DNA replication, covering genes such as PCNA, MCM2, MCM3, MCM4, MCM5 and MCM7...
  23. Lian M, Fang J, Han D, Ma H, Feng L, Wang R, et al. Microarray gene expression analysis of tumorigenesis and regional lymph node metastasis in laryngeal squamous cell carcinoma. PLoS ONE. 2013;8:e84854 pubmed publisher
    ..We found that the six genes (CDK1, CDK2, CDK4, MCM2, MCM3, MCM4) were most frequently differently expressed functional genes related to tumorigenesis while eIF3a and RPN2 were ..
  24. Flück C. MECHANISMS IN ENDOCRINOLOGY: Update on pathogenesis of primary adrenal insufficiency: beyond steroid enzyme deficiency and autoimmune adrenal destruction. Eur J Endocrinol. 2017;177:R99-R111 pubmed publisher
    ..the genetics of some syndromic forms of PAI including IMAGe syndrome (CDKN1C), Irish traveler syndrome (MCM4), MIRAGE syndrome (SAMD9); and most recently a syndrome combining FGD with steroid-resistant nephrotic ..
  25. Gineau L, Cognet C, Kara N, Lach F, Dunne J, Veturi U, et al. Partial MCM4 deficiency in patients with growth retardation, adrenal insufficiency, and natural killer cell deficiency. J Clin Invest. 2012;122:821-32 pubmed publisher
    ..Using linkage analysis and fine mapping, we identified the disease-causing gene, MCM4, which encodes a component of the MCM2-7 helicase complex required for DNA replication...
  26. Sabatinos S, Forsburg S. Managing Single-Stranded DNA during Replication Stress in Fission Yeast. Biomolecules. 2015;5:2123-39 pubmed publisher
    ..Another category of helicase mutant (mcm4-degron) causes fork stalling in early S-phase due to immediate loss of helicase function...
  27. Qian Z, Leung Pineda V, Xuan B, Piwnica Worms H, Yu D. Human cytomegalovirus protein pUL117 targets the mini-chromosome maintenance complex and suppresses cellular DNA synthesis. PLoS Pathog. 2010;6:e1000814 pubmed publisher
    ..HCMV to delay the accumulation of the mini-chromosome maintenance (MCM) complex proteins, represented by MCM2 and MCM4, and prevent their loading onto chromatin, was compromised in the absence of pUL117...
  28. Sidhu K, Kumar V. c-ETS transcription factors play an essential role in the licensing of human MCM4 origin of replication. Biochim Biophys Acta. 2015;1849:1319-28 pubmed publisher
    ..Here we show that ETS transcription factors are novel regulators of MCM4 origin, whose binding sites are localized between two divergently transcribing MCM4 and PRKDC genes...
  29. Melaiu O, Melissari E, Mutti L, Bracci E, De Santi C, Iofrida C, et al. Expression status of candidate genes in mesothelioma tissues and cell lines. Mutat Res. 2015;771:6-12 pubmed publisher
    ..COL1A1, CXADR, EIF4G1, GALNT7, ITGA4, KRT5, PTGIS, RAN, SOD1) in at least one cell line, whereas 7 (DSP, HEG1, MCM4, MSLN, NME2, NMU, TNPO2) were close but did not reach the statistical significance in any of the cell line...
  30. Bakema J, de Haij S, Den Hartog Jager C, Bakker J, Vidarsson G, van Egmond M, et al. Signaling through mutants of the IgA receptor CD89 and consequences for Fc receptor gamma-chain interaction. J Immunol. 2006;176:3603-10 pubmed
    ..This suggests the involvement of additional amino acids in the FcalphaRI-FcR gamma-chain interaction. ..
  31. Zekri A, Hassan Z, Bahnassy A, Khaled H, El Rouby M, Haggag R, et al. Differentially expressed genes in metastatic advanced Egyptian bladder cancer. Asian Pac J Cancer Prev. 2015;16:3543-9 pubmed
    ..different pathways, MDM2 in 9 pathways, FZD4 in 5 pathways, p15 and FGF12 in 4 pathways, POLE2 in 3 pathways, and MCM4 and POLR2E in 2 pathways...
  32. Jazayeri O, Liu X, van Diemen C, Bakker van Waarde W, Sikkema Raddatz B, Sinke R, et al. A novel homozygous insertion and review of published mutations in the NNT gene causing familial glucocorticoid deficiency (FGD). Eur J Med Genet. 2015;58:642-9 pubmed publisher
    ..FGD is a heterogeneous disorder for which causal mutations have been identified in MC2R, MRAP, MCM4 and TXNRD2. Also mutations in STAR and CYP11A1 can sometimes present with a phenotype resembling FGD...
  33. Suman S, Mishra A. An interaction network driven approach for identifying biomarkers for progressing cervical intraepithelial neoplasia. Sci Rep. 2018;8:12927 pubmed publisher
    ..DEGs namely NDC80, ZWINT, CDC7, MCM4, MCM2 and MCM6 were found to be common in both the significant modules as well as the hubs...
  34. Guo W, Wu Z, Song J, Jiang F, Wang Z, Deng S, et al. Juvenile hormone-receptor complex acts on mcm4 and mcm7 to promote polyploidy and vitellogenesis in the migratory locust. PLoS Genet. 2014;10:e1004702 pubmed publisher
    ..b>Mcm4 and Mcm7, two genes in the DNA replication pathway were expressed in the presence of JH and Met...
  35. Guo Y, Fan Y, Zhang J, Chang L, Lin J, Chen Y. Peroxisome proliferator-activated receptor ? coactivator 1? (PGC-1?) protein attenuates vascular lesion formation by inhibition of chromatin loading of minichromosome maintenance complex in smooth muscle cells. J Biol Chem. 2013;288:4625-36 pubmed publisher
    ..Accordingly, overexpression of PGC-1? decreased the expression of minichromosome maintenance 4 (MCM4), which leads to a decreased loading of the MCM complex onto chromatin at the replication origins and decreased ..
  36. Choy B, LaLonde A, Que J, Wu T, Zhou Z. MCM4 and MCM7, potential novel proliferation markers, significantly correlated with Ki-67, Bmi1, and cyclin E expression in esophageal adenocarcinoma, squamous cell carcinoma, and precancerous lesions. Hum Pathol. 2016;57:126-135 pubmed publisher
    ..Our study investigated the correlation between MCM4 and MCM7 expression and Ki-67, Bmi1, and cyclin E expression in esophageal adenocarcinoma, squamous cell carcinoma, ..
  37. Hu B, Burkhart R, Schulte D, Musahl C, Knippers R. The P1 family: a new class of nuclear mammalian proteins related to the yeast Mcm replication proteins. Nucleic Acids Res. 1993;21:5289-93 pubmed
    ..of proteins with high sequence similarities to the yeast proteins Mcm3 and Cdc46 from Saccharomyces cerevisiae and Cdc21 from S. pombe. Our results establish a novel and highly conserved family of nuclear proteins in mammalian cells.
  38. Lim C, Chong S, Jiang Y. Udu deficiency activates DNA damage checkpoint. Mol Biol Cell. 2009;20:4183-93 pubmed publisher
    ..and coimmunoprecipitation data demonstrated that PAH-L repeats and SANT-L domain of Udu interacts with MCM3 and MCM4. Furthermore, Udu is colocalized with 5-bromo-2'-deoxyuridine and heterochromatin during DNA replication, ..
  39. Nakazaki Y, Tsuyama T, Seki M, Takahashi M, Enomoto T, Tada S. Excess Cdt1 inhibits nascent strand elongation by repressing the progression of replication forks in Xenopus egg extracts. Biochem Biophys Res Commun. 2016;470:405-410 pubmed publisher
    ..To identify the mechanism by which Cdt1 inhibits nascent strand elongation, the effect of Cdt1 on loading of Mcm4 and Rpa70 onto chromatin was examined...
  40. Bruck I, Dhingra N, Kaplan D. A Positive Amplification Mechanism Involving a Kinase and Replication Initiation Factor Helps Assemble the Replication Fork Helicase. J Biol Chem. 2017;292:3062-3073 pubmed publisher
    ..We show here that Dpb11 stimulates DDK phosphorylation of the minichromosome maintenance complex protein Mcm4 alone and also of the Mcm2-7 complex and the dsDNA-loaded Mcm2-7 complex...
  41. Ishimi Y, Ichinose S, Omori A, Sato K, Kimura H. Binding of human minichromosome maintenance proteins with histone H3. J Biol Chem. 1996;271:24115-22 pubmed
    ..We detected in HeLa cell extracts six proteins, CDC47, CDC46/MCM5, Cdc21, P1/MCM3, Mis5, and BM28/MCM2, by their binding to a specific antibody and by partial sequencing...
  42. Han J, Liu T, Huen M, Hu L, Chen Z, Huang J. SIVA1 directs the E3 ubiquitin ligase RAD18 for PCNA monoubiquitination. J Cell Biol. 2014;205:811-27 pubmed publisher
    ..Collectively, our results provide evidence that the RAD18 E3 ligase requires an accessory protein for binding to its substrate PCNA. ..
  43. Turnbull A, Arthur L, Renshaw L, Larionov A, Kay C, Dunbier A, et al. Accurate Prediction and Validation of Response to Endocrine Therapy in Breast Cancer. J Clin Oncol. 2015;33:2270-8 pubmed publisher
    ..signaling, and the other [NGFRAP1] was associated with apoptosis) and the level of two proliferation genes (ASPM, MCM4) after 2 weeks of therapy...
  44. Larasati -, Duncker B. Mechanisms Governing DDK Regulation of the Initiation of DNA Replication. Genes (Basel). 2016;8: pubmed publisher
    ..Numerous investigations have determined mechanistic details, including the regions of Mcm2, Mcm4, and Mcm6 phosphorylated by DDK, and a number of DDK docking sites...
  45. O Connell B, Adamson B, Lydeard J, Sowa M, Ciccia A, Bredemeyer A, et al. A genome-wide camptothecin sensitivity screen identifies a mammalian MMS22L-NFKBIL2 complex required for genomic stability. Mol Cell. 2010;40:645-57 pubmed publisher
    ..This study identifies MMS22L-NFKBIL2 as components of the replication stress control pathway and provides a resource for discovery of additional components of this pathway. ..
  46. Sheu Y, Kinney J, Stillman B. Concerted activities of Mcm4, Sld3, and Dbf4 in control of origin activation and DNA replication fork progression. Genome Res. 2016;26:315-30 pubmed publisher
    ..b>Mcm4, a helicase subunit, possesses an unstructured regulatory domain that mediates control from multiple kinase ..
  47. Nan Y, Hu Y, Liu Z, Duan F, Xu Y, Li S, et al. Relationships between cell cycle pathway gene polymorphisms and risk of hepatocellular carcinoma. World J Gastroenterol. 2016;22:5558-67 pubmed publisher
    ..The HCC risk was lower in patients with the MCM4 rs2305952 CC (OR = 0.22, 95%CI: 0.08-0.63, P = 0.01) and with the CHEK1 rs515255 TC, TT, TC/TT (OR = 0.73, 95%CI: 0...
  48. Hiraga S, Ly T, Garzón J, Horejsi Z, Ohkubo Y, Endo A, et al. Human RIF1 and protein phosphatase 1 stimulate DNA replication origin licensing but suppress origin activation. EMBO Rep. 2017;18:403-419 pubmed publisher
    ..MCM helicase subunits that show hyperphosphorylation upon RIF1 depletion, with the regulatory N-terminal domain of MCM4 being particularly strongly affected...
  49. Zhai Y, Cheng E, Wu H, Li N, Yung P, Gao N, et al. Open-ringed structure of the Cdt1-Mcm2-7 complex as a precursor of the MCM double hexamer. Nat Struct Mol Biol. 2017;24:300-308 pubmed publisher
    ..Cdt1 wraps around the N-terminal regions of Mcm2, Mcm6 and Mcm4 to stabilize the whole complex...
  50. Gurvich N, Perna F, Farina A, Voza F, Menendez S, Hurwitz J, et al. L3MBTL1 polycomb protein, a candidate tumor suppressor in del(20q12) myeloid disorders, is essential for genome stability. Proc Natl Acad Sci U S A. 2010;107:22552-7 pubmed publisher
    ..We propose that the loss of L3MBTL1 contributes to the development of 20q(-) hematopoietic malignancies by inducing replicative stress, DNA damage, and genomic instability. ..
  51. Yun H, Hyun S, Park J, Kim B, Kwon H. Widdrol activates DNA damage checkpoint through the signaling Chk2-p53-Cdc25A-p21-MCM4 pathway in HT29 cells. Mol Cell Biochem. 2012;363:281-9 pubmed publisher
    ..Moreover, only the expression level of mini-chromosome maintenance 4 (MCM4) protein, a subunit of the eukaryotic DNA replicative helicase, is rapidly down-regulated in HT29 cells treated ..
  52. Watanabe E, Ohara R, Ishimi Y. Effect of an MCM4 mutation that causes tumours in mouse on human MCM4/6/7 complex formation. J Biochem. 2012;152:191-8 pubmed publisher
    ..the effect of this mutation at level of MCM2-7 interaction, we examined the effect of the same mutation of human MCM4 on the complex formation with MCM6 and MCM7 in insect cells...
  53. Liu C, Zhang Y, Huang T, Cai Y. Identification of transcription factors that may reprogram lung adenocarcinoma. Artif Intell Med. 2017;83:52-57 pubmed publisher
    ..Seven core transcription factors (MCM4, VWF, ECT2, RBMS3, LIMCH1, MYBL2 and FBXL7) are detected, and have been reported to contribute to tumorigenesis of ..
  54. Chen Q, Wang L, Jiang M, Huang J, Jiang Z, Feng H, et al. E2F1 interactive with BRCA1 pathway induces HCC two different small molecule metabolism or cell cycle regulation via mitochondrion or CD4+T to cytosol. J Cell Physiol. 2018;233:1213-1221 pubmed publisher
    ..indirect membrane to cytosol pathway as upstream CCNB2-NUSAP1 activation, feedback TTK-HIST1H2BJ-CENPF, downstream MCM4-TK1 reflecting ATP, and microtubule binding with enrichment of CD4+T-related cell cycle regulation in HCC...
  55. Xie L, Li T, Yang L. E2F2 induces MCM4, CCNE2 and WHSC1 upregulation in ovarian cancer and predicts poor overall survival. Eur Rev Med Pharmacol Sci. 2017;21:2150-2156 pubmed
    ..5) in ovarian cancer cohort in TCGA was identified. The association between E2F2, MCM4, CCNE2 and WHSC1 and overall survival (OS) and recurrence-free survival (RFS) in ovarian cancer patients were ..
  56. Connelly M, Zhang H, Kieleczawa J, Anderson C. The promoters for human DNA-PKcs (PRKDC) and MCM4: divergently transcribed genes located at chromosome 8 band q11. Genomics. 1998;47:71-83 pubmed
    ..comparison with the NCBI nonredundant database revealed the locations of the first 13 exons of the upstream gene, MCM4. MCM4 is an essential component of a protein complex that prevents DNA from being replicated more than once per ..
  57. Ranatunga N, Forsburg S. Characterization of a Novel MMS-Sensitive Allele of Schizosaccharomyces pombe mcm4. G3 (Bethesda). 2016;6:3049-3063 pubmed publisher
    ..Mutations in the Mcm4 subunit are associated with replication stress and double strand breaks in multiple systems...
  58. Yuan Z, Riera A, Bai L, Sun J, Nandi S, Spanos C, et al. Structural basis of Mcm2-7 replicative helicase loading by ORC-Cdc6 and Cdt1. Nat Struct Mol Biol. 2017;24:316-324 pubmed publisher
    ..Flexible Mcm2-7 winged-helix domains (WHDs) engage ORC-Cdc6. A three-domain Cdt1 configuration embraces Mcm2, Mcm4, and Mcm6, thus comprising nearly half of the hexamer...
  59. Deegan T, Yeeles J, Diffley J. Phosphopeptide binding by Sld3 links Dbf4-dependent kinase to MCM replicative helicase activation. EMBO J. 2016;35:961-73 pubmed publisher
    ..Sld3 binds specifically to DDK-phosphorylated peptides from two MCM subunits (Mcm4, 6) and then recruits Cdc45...
  60. You Z, Ishimi Y, Masai H, Hanaoka F. Roles of Mcm7 and Mcm4 subunits in the DNA helicase activity of the mouse Mcm4/6/7 complex. J Biol Chem. 2002;277:42471-9 pubmed
    ..A subassembly of Mcm, the Mcm4/6/7 double-trimeric complex, possesses DNA helicase activity, and it has been proposed that Mcm may function as a ..
  61. Hughes C, Guasti L, Meimaridou E, Chuang C, Schimenti J, King P, et al. MCM4 mutation causes adrenal failure, short stature, and natural killer cell deficiency in humans. J Clin Invest. 2012;122:814-20 pubmed publisher
    ..71-1insG) in minichromosome maintenance-deficient 4 (MCM4) that was predicted to result in a severely truncated protein (p.Pro24ArgfsX4)...
  62. Lecona E, Rodriguez Acebes S, Specks J, López Contreras A, Ruppen I, Murga M, et al. USP7 is a SUMO deubiquitinase essential for DNA replication. Nat Struct Mol Biol. 2016;23:270-7 pubmed publisher
  63. Wu Z, Guo W, Xie Y, Zhou S. Juvenile Hormone Activates the Transcription of Cell-division-cycle 6 (Cdc6) for Polyploidy-dependent Insect Vitellogenesis and Oogenesis. J Biol Chem. 2016;291:5418-27 pubmed publisher
    ..composed of methoprene-tolerant and steroid receptor co-activator, acts on mini-chromosome maintenance (Mcm) genes Mcm4 and Mcm7 to promote DNA replication and polyploidy for the massive vitellogenin (Vg) synthesis required for egg ..
  64. Leontovich A, Intine R, Sarras M. Epigenetic Studies Point to DNA Replication/Repair Genes as a Basis for the Heritable Nature of Long Term Complications in Diabetes. J Diabetes Res. 2016;2016:2860780 pubmed publisher found that genes of the DNA replication/DNA metabolism process group (with upregulation of the apex1, mcm2, mcm4, orc3, lig1, and dnmt1 genes) were altered in the DM state and these molecular changes continued into MM...
  65. Luo M, Li D, Wang Z, Guo W, Kang L, Zhou S. Juvenile hormone differentially regulates two Grp78 genes encoding protein chaperones required for insect fat body cell homeostasis and vitellogenesis. J Biol Chem. 2017;292:8823-8834 pubmed publisher
    ..We found that Grp78-1 is regulated by JH through Mcm4/7-dependent DNA replication and polyploidization, whereas Grp78-2 expression is directly activated by the JH-..
  66. Stafford J, Dyson G, Levin N, Chaudhry S, Rosati R, Kalpage H, et al. Reanalysis of BRCA1/2 negative high risk ovarian cancer patients reveals novel germline risk loci and insights into missing heritability. PLoS ONE. 2017;12:e0178450 pubmed publisher
    ..pathways; DNA repair and cell cycle control, including CHEK1, TP53I3, REC8, HMMR, RAD52, RAD1, POLK, POLQ, and MCM4. In summary, our study implicates novel risk loci as well as highlights the clinical utility for retesting BRCA1/2 ..
  67. Polotskaia A, Xiao G, Reynoso K, Martin C, Qiu W, Hendrickson R, et al. Proteome-wide analysis of mutant p53 targets in breast cancer identifies new levels of gain-of-function that influence PARP, PCNA, and MCM4. Proc Natl Acad Sci U S A. 2015;112:E1220-9 pubmed publisher
    ..Mtp53 increased the level of proliferating cell nuclear antigen (PCNA) and minichromosome maintenance 4 (MCM4) proteins without changing the amount of pcna and mcm4 transcripts...
  68. Han J, Lian M, Fang J, Liu H, Wang R, Zhai J, et al. Minichromosome maintenance (MCM) protein 4 overexpression is a potential prognostic marker for laryngeal squamous cell carcinoma. J BUON. 2017;22:1272-1277 pubmed
    The minichromosomal maintenance (MCM) proteins are involved in the initiation and DNA replication. The role of MCM4 remains to be elucidated...
  69. You Z, Komamura Y, Ishimi Y. Biochemical analysis of the intrinsic Mcm4-Mcm6-mcm7 DNA helicase activity. Mol Cell Biol. 1999;19:8003-15 pubmed
    ..Recently, we reported that a DNA helicase activity is associated with an Mcm4-Mcm6-Mcm7 (Mcm4,6,7) complex, suggesting that this complex is involved in the initiation of DNA replication as a ..
  70. Clarke C, Clarke P. DNA-dependent phosphorylation of Chk1 and Claspin in a human cell-free system. Biochem J. 2005;388:705-12 pubmed
    ..This cell-free system will facilitate further biochemical analysis of the Chk1 pathway in humans. ..
  71. Wei L, Wang Q, Zhang Y, Yang C, Guan H, Chen Y, et al. Identification of key genes, transcription factors and microRNAs involved in intracranial aneurysm. Mol Med Rep. 2017;: pubmed publisher
    ..of miRNA?125b, and myosin heavy chain 11 (MYH11), a target of minichromosome maintenance complex component 4 (MCM4), had higher degree scores in the integrated network...
  72. Chu Y, Ko C, Wang S, Lin P, Wang H, Lin W, et al. Bortezomib-induced miRNAs direct epigenetic silencing of locus genes and trigger apoptosis in leukemia. Cell Death Dis. 2017;8:e3167 pubmed publisher
    ..including protein kinase DNA-activated catalytic polypeptide (PRKDC), minichromosome maintenance-deficient 4 (MCM4) and ubiquitin-conjugating enzyme E2 variant 2 (UBE2V2), upon bortezomib treatment...
  73. Ladenburger E, Fackelmayer F, Hameister H, Knippers R. MCM4 and PRKDC, human genes encoding proteins MCM4 and DNA-PKcs, are close neighbours located on chromosome 8q12-->q13. Cytogenet Cell Genet. 1997;77:268-70 pubmed
    A human genomic DNA fragment containing the 5' region of MCM4, a gene encoding replication protein MCM4/Cdc21 was isolated...
  74. Izumi M, Yanagi K, Mizuno T, Yokoi M, Kawasaki Y, Moon K, et al. The human homolog of Saccharomyces cerevisiae Mcm10 interacts with replication factors and dissociates from nuclease-resistant nuclear structures in G(2) phase. Nucleic Acids Res. 2000;28:4769-77 pubmed
    ..HsMcm10 also interacted with Orc2, Mcm2 and Mcm6 proteins in the yeast two-hybrid system. These results suggest that HsMcm10 may function in DNA replication through the interaction with Orc and Mcm2-7 complexes. ..
  75. Yabuta N, Kajimura N, Mayanagi K, Sato M, Gotow T, Uchiyama Y, et al. Mammalian Mcm2/4/6/7 complex forms a toroidal structure. Genes Cells. 2003;8:413-21 pubmed
    ..They form Mcm4/6/7 and Mcm2/4/6/7 complexes, but their structures are not known...
  76. Ishimi Y, Komamura Kohno Y, Karasawa Shimizu K, Yamada K. Levels of MCM4 phosphorylation and DNA synthesis in DNA replication block checkpoint control. J Struct Biol. 2004;146:234-41 pubmed
    ..We reported that MCM4, a subunit of a putative replicative DNA helicase, is extensively phosphorylated in the presence of hydroxyurea (HU)..
  77. Huang X, Zhang X, Su X, Ma G, Zhao J, Rong T. [Expression and significance of MCM4 in esophageal cancer]. Ai Zheng. 2007;26:96-9 pubmed
    b>MCM4 belongs to minichromosome maintenance (MCM) protein family. It is a DNA replication licensing factor. This study was to explore the expression and significance of MCM4 in esophageal squamous cell cancer (ESCC)...
  78. Lv L, Wang F, Ma X, Yang Y, Wang Z, Liu H, et al. Mismatch repair protein MSH2 regulates translesion DNA synthesis following exposure of cells to UV radiation. Nucleic Acids Res. 2013;41:10312-22 pubmed publisher
    ..Moreover, we observed that MSH2 can facilitate TLS across cyclobutane pyrimidine dimers photoproducts in living cells, presenting a novel role of MSH2 in post-UV cellular responses. ..
  79. Schulte D, Richter A, Burkhart R, Musahl C, Knippers R. Properties of the human nuclear protein p85Mcm. Expression, nuclear localization and interaction with other Mcm proteins. Eur J Biochem. 1996;235:144-51 pubmed
    ..The complex dissociates at high ionic strength giving rise to stable subcomplexes, one of which contains protein p85Mcm together with Mcm proteins hCdc21 and p1O5Mcm.
  80. Schmitz N, Leibundgut K, Hirt A. MCM4 shares homology to a replication/DNA-binding domain in CTF and is contacted by pRb. Biochem Biophys Res Commun. 2004;317:779-86 pubmed
    ..A new binding protein of under-phosphorylated pRb, MCM4, was identified using LexA-Rb(561-660) in the two-hybrid assay...
  81. Tan B, Chien C, Hirose S, Lee S. Functional cooperation between FACT and MCM helicase facilitates initiation of chromatin DNA replication. EMBO J. 2006;25:3975-85 pubmed
  82. Vijayraghavan S, Schwacha A. The eukaryotic Mcm2-7 replicative helicase. Subcell Biochem. 2012;62:113-34 pubmed publisher
  83. Tatsumi R, Ishimi Y. An MCM4 mutation detected in cancer cells affects MCM4/6/7 complex formation. J Biochem. 2017;161:259-268 pubmed publisher
    An MCM4 mutation detected in human cancer cells from endometrium was characterized...
  84. Musahl C, Schulte D, Burkhart R, Knippers R. A human homologue of the yeast replication protein Cdc21. Interactions with other Mcm proteins. Eur J Biochem. 1995;230:1096-101 pubmed
    ..Specific antibodies, raised against protein hCdc21, were used to investigate the expression of the protein through the cell cycle...
  85. Lee J, Hurwitz J. Processive DNA helicase activity of the minichromosome maintenance proteins 4, 6, and 7 complex requires forked DNA structures. Proc Natl Acad Sci U S A. 2001;98:54-9 pubmed
    ..The presence of both 5' and 3' tails facilitated the formation of double heterohexameric complexes of Mcm4/6/7 on substrate DNA, which appeared to be essential for the processive helicase activity...
  86. DaFonseca C, Shu F, Zhang J. Identification of two residues in MCM5 critical for the assembly of MCM complexes and Stat1-mediated transcription activation in response to IFN-gamma. Proc Natl Acad Sci U S A. 2001;98:3034-9 pubmed
    ..Together, these results strongly suggest that Stat1 recruits a MCM5/3 subcomplex through direct interaction with MCM5 in the process of IFN-gamma-induced gene activation. ..
  87. Sar F, Lindsey Boltz L, Subramanian D, Croteau D, Hutsell S, Griffith J, et al. Human claspin is a ring-shaped DNA-binding protein with high affinity to branched DNA structures. J Biol Chem. 2004;279:39289-95 pubmed
  88. Karmakar S, Mahajan M, Schulz V, Boyapaty G, Weissman S. A multiprotein complex necessary for both transcription and DNA replication at the ?-globin locus. EMBO J. 2010;29:3260-71 pubmed publisher
    ..These results imply a direct link between mammalian DNA replication, transcription and histone acetylation mediated by a single multiprotein complex. ..
  89. Huang T, Huo L, Wang Y, Xia W, Wei Y, Chang S, et al. Epidermal growth factor receptor potentiates MCM7-mediated DNA replication through tyrosine phosphorylation of Lyn kinase in human cancers. Cancer Cell. 2013;23:796-810 pubmed publisher
    ..These results establish a signaling cascade in which EGFR enhances MCM7 phosphorylation and DNA replication through Lyn phosphorylation in human cancer cells. ..
  90. Lossaint G, Larroque M, Ribeyre C, Bec N, Larroque C, Décaillet C, et al. FANCD2 binds MCM proteins and controls replisome function upon activation of s phase checkpoint signaling. Mol Cell. 2013;51:678-90 pubmed publisher
    ..These data reveal that FANCD2 is an effector of ATR signaling implicated in a general replisome surveillance mechanism that is necessary for sustaining cell proliferation and attenuating carcinogenesis. ..
  91. Kusunoki S, Ishimi Y. Interaction of human minichromosome maintenance protein-binding protein with minichromosome maintenance 2-7. FEBS J. 2014;281:1057-67 pubmed publisher
    ..These results suggest that MCM-BP functions in disassembling MCM2-7 on chromatin during S phase and G2 phase by interacting with MCM3, MCM5, and MCM7. ..
  92. Herbig U, Marlar C, Fanning E. The Cdc6 nucleotide-binding site regulates its activity in DNA replication in human cells. Mol Biol Cell. 1999;10:2631-45 pubmed
    ..Microinjection of either mutant protein into human cells in G1 inhibited DNA replication, suggesting that ATP binding and hydrolysis by HsCdc6 are essential for DNA replication. ..