Gene Symbol: MBD3
Description: methyl-CpG binding domain protein 3
Alias: methyl-CpG-binding domain protein 3
Species: human
Products:     MBD3

Top Publications

  1. Hendrich B, Bird A. Identification and characterization of a family of mammalian methyl-CpG binding proteins. Mol Cell Biol. 1998;18:6538-47 pubmed
    ..We describe here three novel human and mouse proteins (MBD2, MBD3, and MBD4) that contain the methyl-CpG binding domain. MBD2 and MBD4 bind specifically to methylated DNA in vitro...
  2. Zhang Y, LeRoy G, Seelig H, Lane W, Reinberg D. The dermatomyositis-specific autoantigen Mi2 is a component of a complex containing histone deacetylase and nucleosome remodeling activities. Cell. 1998;95:279-89 pubmed
    ..The finding that Mi2beta exists in a complex containing histone deacetylase and nucleosome remodeling activities suggests a role for chromatin reorganization in cancer metastasis. ..
  3. Morey L, Brenner C, Fazi F, Villa R, Gutierrez A, Buschbeck M, et al. MBD3, a component of the NuRD complex, facilitates chromatin alteration and deposition of epigenetic marks. Mol Cell Biol. 2008;28:5912-23 pubmed publisher
  4. Spruijt C, Bartels S, Brinkman A, Tjeertes J, Poser I, Stunnenberg H, et al. CDK2AP1/DOC-1 is a bona fide subunit of the Mi-2/NuRD complex. Mol Biosyst. 2010;6:1700-6 pubmed publisher
    ..Finally, immunofluorescence experiments reveal co-localization between MBD2 and DOC-1 in mouse NIH-3T3 nuclei. Collectively, these results indicate that DOC-1 is a bona fide subunit of the Mi-2/NuRD chromatin remodeling complex. ..
  5. Van Rechem C, Boulay G, Pinte S, Stankovic Valentin N, Guerardel C, Leprince D. Differential regulation of HIC1 target genes by CtBP and NuRD, via an acetylation/SUMOylation switch, in quiescent versus proliferating cells. Mol Cell Biol. 2010;30:4045-59 pubmed publisher
    ..Our results decipher a mechanistic link between two competitive posttranslational modifications of HIC1 and corepressor recruitment to specific genes, leading to growth control. ..
  6. Zhang Y, Ng H, Erdjument Bromage H, Tempst P, Bird A, Reinberg D. Analysis of the NuRD subunits reveals a histone deacetylase core complex and a connection with DNA methylation. Genes Dev. 1999;13:1924-35 pubmed
    ..highly related to the metastasis-associated protein 1, MTA2, and the methyl-CpG-binding domain-containing protein, MBD3, were found to be subunits of the NuRD complex...
  7. Saito M, Ishikawa F. The mCpG-binding domain of human MBD3 does not bind to mCpG but interacts with NuRD/Mi2 components HDAC1 and MTA2. J Biol Chem. 2002;277:35434-9 pubmed
    Although mammalian MBD3 contains the mCpG-binding domain (MBD) and is highly homologous with the authentic mCpG-binding protein MBD2, it was reported that the protein does not bind to mCpG specifically...
  8. Tong J, Hassig C, Schnitzler G, Kingston R, Schreiber S. Chromatin deacetylation by an ATP-dependent nucleosome remodelling complex. Nature. 1998;395:917-21 pubmed
    ..Our results establish a physical and functional link between the distinct chromatin-modifying activities of histone deacetylases and nucleosome remodelling proteins. ..
  9. Brackertz M, Boeke J, Zhang R, Renkawitz R. Two highly related p66 proteins comprise a new family of potent transcriptional repressors interacting with MBD2 and MBD3. J Biol Chem. 2002;277:40958-66 pubmed
    Methyl-CpG-binding domain proteins (MBD) mediate functional responses of methylated DNA. MBD2 and MBD3 are components of the MeCP1 protein complex, which contains the Mi-2/NuRD complex and includes 66- and 68-kDa polypeptides...

More Information

Publications103 found, 100 shown here

  1. Günther K, Rust M, Leers J, Boettger T, Scharfe M, Jarek M, et al. Differential roles for MBD2 and MBD3 at methylated CpG islands, active promoters and binding to exon sequences. Nucleic Acids Res. 2013;41:3010-21 pubmed publisher
    ..collection of nucleosome remodelling and deacetylation (NuRD) complexes can be grouped into the MBD2- or MBD3-containing complexes MBD2-NuRD and MBD3-NuRD...
  2. Le Guezennec X, Vermeulen M, Brinkman A, Hoeijmakers W, Cohen A, Lasonder E, et al. MBD2/NuRD and MBD3/NuRD, two distinct complexes with different biochemical and functional properties. Mol Cell Biol. 2006;26:843-51 pubmed
    ..To gain insight into the function of MBD2 and MBD3, we first applied protein tagging and mass spectrometry...
  3. Yildirim O, Li R, Hung J, Chen P, Dong X, Ee L, et al. Mbd3/NURD complex regulates expression of 5-hydroxymethylcytosine marked genes in embryonic stem cells. Cell. 2011;147:1498-510 pubmed publisher
    ..We find that Mbd3 and Brg1 antagonistically regulate a common set of genes by regulating promoter nucleosome occupancy...
  4. Feng Q, Cao R, Xia L, Erdjument Bromage H, Tempst P, Zhang Y. Identification and functional characterization of the p66/p68 components of the MeCP1 complex. Mol Cell Biol. 2002;22:536-46 pubmed
    ..is involved in the association of p66 with other MeCP1 components, CR2 plays an important role in targeting p66 and MBD3 to specific loci...
  5. Fujita N, Jaye D, Kajita M, Geigerman C, Moreno C, Wade P. MTA3, a Mi-2/NuRD complex subunit, regulates an invasive growth pathway in breast cancer. Cell. 2003;113:207-19 pubmed
    ..These results establish a mechanistic link between estrogen receptor status and invasive growth of breast cancers. ..
  6. LeShane E, Shinde U, Walker J, Barry A, Blackburn N, Ralle M, et al. Interactions between copper-binding sites determine the redox status and conformation of the regulatory N-terminal domain of ATP7B. J Biol Chem. 2010;285:6327-36 pubmed publisher
    ..Mutating MBD2 or MBD3 causes Cys oxidation in other MBDs and loss of copper binding...
  7. Veldhuis N, Kuiper M, Dobson R, Pearson R, Camakaris J. In silico modeling of the Menkes copper-translocating P-type ATPase 3rd metal binding domain predicts that phosphorylation regulates copper-binding. Biometals. 2011;24:477-87 pubmed publisher
    ..We have previously shown that the third copper-binding domain, MBD3, uniquely contains two phosphorylated residues: Thr-327, which is phosphorylated only in the presence of elevated ..
  8. Zviran A, Mor N, Rais Y, Gingold H, Peles S, Chomsky E, et al. Deterministic Somatic Cell Reprogramming Involves Continuous Transcriptional Changes Governed by Myc and Epigenetic-Driven Modules. Cell Stem Cell. 2019;24:328-341.e9 pubmed publisher
    ..Here, we characterize conversion of mouse fibroblasts into iPSCs using Gatad2a-Mbd3/NuRD-depleted and highly efficient reprogramming systems...
  9. Rodríguez Martín M, Martin Ezquerra G, Man M, Hupe M, Youm J, Mackenzie D, et al. Expression of epidermal CAMP changes in parallel with permeability barrier status. J Invest Dermatol. 2011;131:2263-70 pubmed publisher
    ..Moreover, three antimicrobial peptides (AMPs), i.e., mouse β-defensin 3 (mBD3), mouse cathelicidin antimicrobial peptide (mCAMP), and the neuroendocrine peptide, catestatin (Cst), all localize ..
  10. Peng L, Li Y, Xi Y, Li W, Li J, Lv R, et al. MBD3L2 promotes Tet2 enzymatic activity for mediating 5-methylcytosine oxidation. J Cell Sci. 2016;129:1059-71 pubmed publisher
    ..Here, we found that methyl-CpG-binding domain protein 3 (MBD3) and its homolog MBD3-like 2 (MBD3L2) can specifically modulate the enzymatic activity of Tet2 protein, but not ..
  11. Zhu D, Fang J, Li Y, Zhang J. Mbd3, a component of NuRD/Mi-2 complex, helps maintain pluripotency of mouse embryonic stem cells by repressing trophectoderm differentiation. PLoS ONE. 2009;4:e7684 pubmed publisher
    ..Here, we investigate functions of Mbd3, a component of nucleosome remodeling and histone deacetylation complex (NuRD/Mi-2) in mouse ES cells...
  12. Ekanayaka S, McClellan S, Barrett R, Kharotia S, Hazlett L. Glycyrrhizin Reduces HMGB1 and Bacterial Load in Pseudomonas aeruginosa Keratitis. Invest Ophthalmol Vis Sci. 2016;57:5799-5809 pubmed publisher
    ..Treatment with GLY enhanced antimicrobial proteins, including CRAMP and mBD2, but not mBD3. Glycyrrhizin also reduced clinical scores and improved disease outcome in corneas infected with strain 19660...
  13. Tsuboi M, Kishi Y, Yokozeki W, Koseki H, Hirabayashi Y, Gotoh Y. Ubiquitination-Independent Repression of PRC1 Targets during Neuronal Fate Restriction in the Developing Mouse Neocortex. Dev Cell. 2018;47:758-772.e5 pubmed publisher
    ..Our results also suggest that histone deacetylase (HDAC) activity of the NuRD/MBD3 complex and Phc2-dependent PRC1 clustering are necessary for the transition from the Ub-dependent to -independent ..
  14. Jung Y, Jung M, Kim M, Hong S, Choi E. IL-1α stimulation restores epidermal permeability and antimicrobial barriers compromised by topical tacrolimus. J Invest Dermatol. 2011;131:698-705 pubmed publisher
    ..in an increase in the production of LB and three major lipid synthesis-related enzymes, and in the expressions of mBD3, CRAMP, and IL-1α (n=5)...
  15. Smith J, Aldous S, Andreassi C, Cuda G, Gaspari M, Riccio A. Proteomic analysis of S-nitrosylated nuclear proteins in rat cortical neurons. Sci Signal. 2018;11: pubmed publisher
    ..addition, lysine motifs were necessary for promoting the S-nitrosylation of HDAC2 and methyl-CpG binding protein 3 (MBD3)...
  16. Hirota A, Nakajima Koyama M, Ashida Y, Nishida E. The nucleosome remodeling and deacetylase complex protein CHD4 regulates neural differentiation of mouse embryonic stem cells by down-regulating p53. J Biol Chem. 2019;294:195-209 pubmed publisher
    ..i>Chd4 knockdown suppresses neural differentiation, as did knockdown of methyl-CpG-binding domain protein Mbd3, another NuRD subunit...
  17. Ozmadenci D, Féraud O, Markossian S, Kress E, Ducarouge B, Gibert B, et al. Netrin-1 regulates somatic cell reprogramming and pluripotency maintenance. Nat Commun. 2015;6:7398 pubmed publisher
    ..we found that reprogramming is accompanied by a transient transcriptional repression of Netrin-1 mediated by an Mbd3/Mta1/Chd4-containing NuRD complex...
  18. Kolar S, Baidouri H, Hanlon S, McDermott A. Protective role of murine β-defensins 3 and 4 and cathelin-related antimicrobial peptide in Fusarium solani keratitis. Infect Immun. 2013;81:2669-77 pubmed publisher
    ..In this study, we investigated the protective effect of murine β-defensin 3 (mBD3), mBD4, and the cathelicidin cathelin-related antimicrobial peptide (CRAMP) in a murine model of Fusarium solani ..
  19. Xu M, Bian S, Li J, He J, Chen H, Ge L, et al. MeCP2 suppresses LIN28A expression via binding to its methylated-CpG islands in pancreatic cancer cells. Oncotarget. 2016;7:14476-85 pubmed publisher
    ..protein 2 (MeCP2) binds preferentially to the two hypermethylated CpG islands sites at LIN28A promoter compare to MBD3. Expectedly, MeCP2 knockdown transcriptionally activates LIN28A expression in above cells, rather than MBD3 ..
  20. Li X, Yan Q, Tang S, Tan Z, Fitzsimmons C, Yi K. Effects of maternal feed intake restriction during pregnancy on the expression of growth regulation, imprinting and epigenetic transcription-related genes in foetal goats. Anim Reprod Sci. 2018;198:90-98 pubmed publisher
    ..relative abundance of mRNA of methyl-CpG-binding domain protein 2 (MBD2) and methyl-CpG-binding domain protein 3 (MBD3) genes in the foetal liver were greater (P <  0.05) in the RG...
  21. Zhang T, Wei G, Millard C, Fischer R, Konietzny R, Kessler B, et al. A variant NuRD complex containing PWWP2A/B excludes MBD2/3 to regulate transcription at active genes. Nat Commun. 2018;9:3798 pubmed publisher
    ..PWWP2A competes with MBD3 for binding to MTA1, thus defining a new variant NuRD complex that is mutually exclusive with the MBD2/3 containing ..
  22. Dos Santos R, Tosti L, Radzisheuskaya A, Caballero I, Kaji K, Hendrich B, et al. MBD3/NuRD facilitates induction of pluripotency in a context-dependent manner. Cell Stem Cell. 2014;15:102-10 pubmed publisher
    ..By knocking out MBD3, an essential scaffold subunit of the NuRD complex, at different time points in reprogramming, we found that ..
  23. Kaji K, Nichols J, Hendrich B. Mbd3, a component of the NuRD co-repressor complex, is required for development of pluripotent cells. Development. 2007;134:1123-32 pubmed
    b>Mbd3 is a core component of the NuRD (Nucleosome Remodeling and Histone Deacetylation) co-repressor complex, and NuRD-mediated silencing has been implicated in cell fate decisions in a number of contexts...
  24. Gowda R, REDFERN R, Frikeche J, Pinglay S, Foster J, Lema C, et al. Functions of Peptidoglycan Recognition Proteins (Pglyrps) at the Ocular Surface: Bacterial Keratitis in Gene-Targeted Mice Deficient in Pglyrp-2, -3 and -4. PLoS ONE. 2015;10:e0137129 pubmed publisher
    ..Transcripts for antimicrobial peptides mBD2, mBD3 and CRAMP were elevated in infected Pglyrp-2-/- males without statistical significance...
  25. Yan X, Liu H, Liu J, Zhang R, Wang G, Li Q, et al. Evidence in duck for supporting alteration of incubation temperature may have influence on methylation of genomic DNA. Poult Sci. 2015;94:2537-45 pubmed publisher
    ..reprogrammed by DNA (cytosine-5)-methyltransferases (DNMTS) and Methyl-CpG binding domain proteins (MBPS) 3&5 (MBD3&5)...
  26. Knock E, Pereira J, Lombard P, Dimond A, Leaford D, Livesey F, et al. The methyl binding domain 3/nucleosome remodelling and deacetylase complex regulates neural cell fate determination and terminal differentiation in the cerebral cortex. Neural Dev. 2015;10:13 pubmed publisher
    ..The methyl binding domain 3/nucleosome remodelling and deacetylation (MBD3/NuRD) co-repressor complex facilitates lineage commitment of pluripotent cells in early mouse embryos and is ..
  27. Mor N, Rais Y, Sheban D, Peles S, Aguilera Castrejon A, Zviran A, et al. Neutralizing Gatad2a-Chd4-Mbd3/NuRD Complex Facilitates Deterministic Induction of Naive Pluripotency. Cell Stem Cell. 2018;23:412-425.e10 pubmed publisher
    b>Mbd3, a member of nucleosome remodeling and deacetylase (NuRD) co-repressor complex, was previously identified as an inhibitor for deterministic induced pluripotent stem cell (iPSC) reprogramming, where up to 100% of donor cells ..
  28. Drozd A, Walczak M, Piaskowski S, Stoczyńska Fidelus E, Rieske P, Grzela D. Generation of human iPSCs from cells of fibroblastic and epithelial origin by means of the oriP/EBNA-1 episomal reprogramming system. Stem Cell Res Ther. 2015;6:122 pubmed publisher
    ..system enhanced reprogramming efficiencies in fibroblasts and epithelial cells, whereas the downregulation of Mbd3 expression increased iPSC colony-forming efficiency in fibroblasts solely...
  29. Luo Z, Gao X, Lin C, Smith E, Marshall S, Swanson S, et al. Zic2 is an enhancer-binding factor required for embryonic stem cell specification. Mol Cell. 2015;57:685-94 pubmed publisher
    ..Detailed genome-wide and molecular studies reveal that Zic2 can function with Mbd3/NuRD in regulating the chromatin state and transcriptional output of genes linked to differentiation...
  30. Menafra R, Stunnenberg H. MBD2 and MBD3: elusive functions and mechanisms. Front Genet. 2014;5:428 pubmed publisher
    ..This review focuses on two members of the methyl binding proteins, namely MBD2 and MBD3 that reside in very similar complexes, yet appear to have very different biological roles...
  31. Hainer S, Fazzio T. Regulation of Nucleosome Architecture and Factor Binding Revealed by Nuclease Footprinting of the ESC Genome. Cell Rep. 2015;13:61-69 pubmed publisher
    ..the binding sites of 30 regulatory factors in mouse embryonic stem cells (ESCs), we provide evidence that esBAF and Mbd3/NuRD modulate the binding of several regulatory proteins...
  32. Zhao H, Han Z, Liu X, Gu J, Tang F, Wei G, et al. The chromatin remodeler Chd4 maintains embryonic stem cell identity by controlling pluripotency- and differentiation-associated genes. J Biol Chem. 2017;292:8507-8519 pubmed publisher
    ..The phenotype was different from that caused by the deletion of Mbd3, another subunit of the NuRD complex...
  33. Paolini N, Moore K, di Summa F, Fokkema I, t Hoen P, von Lindern M. Ribosome profiling uncovers selective mRNA translation associated with eIF2 phosphorylation in erythroid progenitors. PLoS ONE. 2018;13:e0193790 pubmed publisher
    ..was increased for 147 transcripts, among which transcriptional regulators such as Atf4, Tis7/Ifrd1, Pnrc2, Gtf2h, Mbd3, JunB and Kmt2e. Translation of 337 transcripts decreased more than average, among which Dym and Csde1...
  34. Zhang Y, Zhao Y, Liu H, Yu W, Yang F, Li W, et al. Mouse β-Defensin 3, A Defensin Inhibitor of Both Its Endogenous and Exogenous Potassium Channels. Molecules. 2018;23: pubmed publisher
    ..In this work, we reported that the mouse &beta;-defensin 3 (mBD3) was a novel inhibitor of both endogenous and exogenous potassium channels...
  35. Saenz de Juano M, Billooye K, Smitz J, Anckaert E. The loss of imprinted DNA methylation in mouse blastocysts is inflicted to a similar extent by in vitro follicle culture and ovulation induction. Mol Hum Reprod. 2016;22:427-41 pubmed publisher
    ..genes in the oocyte, a comparison of DNA methyltransferase 1 (Dnmt1o), methyl-CpG binding domain protein 3 (MBD3) and developmental pluripotency-associated 3 (Dppa3) was performed by qPCR between in vivo and in vitro grown ..
  36. Hendrich B, Abbott C, McQueen H, Chambers D, Cross S, Bird A. Genomic structure and chromosomal mapping of the murine and human Mbd1, Mbd2, Mbd3, and Mbd4 genes. Mamm Genome. 1999;10:906-12 pubmed
    ..MeCP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of mammalian, nuclear proteins related by the presence in each of an amino acid motif ..
  37. Zhang L, Zheng Y, Sun Y, Zhang Y, Yan J, Chen Z, et al. MiR-134-Mbd3 axis regulates the induction of pluripotency. J Cell Mol Med. 2016;20:1150-8 pubmed publisher
    ..And Mbd3 was found to promote the induction of iPSCs and could block the repression of reprogramming caused by ..
  38. Moon B, Yun H, Chang W, Steele B, Cai M, Choi S, et al. Smek promotes corticogenesis through regulating Mbd3's stability and Mbd3/NuRD complex recruitment to genes associated with neurogenesis. PLoS Biol. 2017;15:e2001220 pubmed publisher
    ..Here, we demonstrate that Suppressor of Mek null (Smek) interact with methyl-CpG-binding domain 3 (Mbd3) and the complex plays a critical role in self-renewal and neuronal differentiation of NPCs...
  39. Zhao S, Bellone S, Lopez S, Thakral D, Schwab C, English D, et al. Mutational landscape of uterine and ovarian carcinosarcomas implicates histone genes in epithelial-mesenchymal transition. Proc Natl Acad Sci U S A. 2016;113:12238-12243 pubmed
    ..We also found frequent deletions of the genes TP53 and MBD3 (a member with CHD4 of the nucleosome remodeling deacetylase complex) and frequent amplification of chromosome ..
  40. Sanna M, Galeotti N. The HDAC1/c-JUN complex is essential in the promotion of nerve injury-induced neuropathic pain through JNK signaling. Eur J Pharmacol. 2018;825:99-106 pubmed publisher
    ..No variation in HDAC3, DNMT3a, AcH3, MBD3 and MeCP2 levels was detected...
  41. Nagamatsu G, Saito S, Takubo K, Suda T. Integrative Analysis of the Acquisition of Pluripotency in PGCs Reveals the Mutually Exclusive Roles of Blimp-1 and AKT Signaling. Stem Cell Reports. 2015;5:111-24 pubmed publisher
    ..AKT played a major role in suppressing genes regulated by MBD3. From these results, we defined the distinct functions of Blimp-1 and Akt and provided mechanistic insights into ..
  42. Zhang W, Aubert A, Gomez de Segura J, Karuppasamy M, Basu S, Murthy A, et al. The Nucleosome Remodeling and Deacetylase Complex NuRD Is Built from Preformed Catalytically Active Sub-modules. J Mol Biol. 2016;428:2931-42 pubmed publisher
    ..imaging of CHD4 in live mouse embryonic stem cells, in the presence and absence of one of core components (MBD3), to show how the core deacetylase and chromatin-remodeling sub-modules associate in vivo...
  43. Bednarczyk J, Dębski K, Bot A, Lukasiuk K. MBD3 expression and DNA binding patterns are altered in a rat model of temporal lobe epilepsy. Sci Rep. 2016;6:33736 pubmed publisher
    The aim of the present study was to examine involvement of MBD3 (methyl-CpG-binding domain protein 3), a protein involved in reading DNA methylation patterns, in epileptogenesis and epilepsy...
  44. Tommasi S, Zheng A, Besaratinia A. Expression of epigenetic modifiers is not significantly altered by exposure to secondhand smoke. Lung Cancer. 2015;90:598-603 pubmed publisher
    ..encoding DNA methyltransferases (Dnmt1, Dnmt3a, Dnmt3b and Dnmt3l), methyl binding domain proteins (Mecp2, Mbd2 and Mbd3) and histone deacetylases (Hdac1 and Hdac2) by quantitative reverse-transcription polymerase chain reaction ..
  45. Wang J, Lu J, Xie X, Xiong J, Huang N, Wei H, et al. Blend of organic acids and medium chain fatty acids prevents the inflammatory response and intestinal barrier dysfunction in mice challenged with enterohemorrhagic Escherichia coli O157:H7. Int Immunopharmacol. 2018;58:64-71 pubmed publisher
    ..of tight junction proteins occludin and ZO-1, mucus protein MUC-2, and host defense peptides mBD1, mBD2 and mBD3. Finally, OM1 and OM2 increased the population of Lactobacillus spp. and Bifidobacterium spp...
  46. Wang Y, Lin Z, Zheng H, Zhang S, Zhang Z, Chen Y, et al. Abnormal DNA methylation in the lumbar spinal cord following chronic constriction injury in rats. Neurosci Lett. 2016;610:1-5 pubmed publisher
    ..and methyl-CpG binding protein 2 (MeCP2) expression increased, MBD2 expression decreased, and DNMT1, MBD1 and MBD3 expression hardly changed in the lumbar spinal cord in CCI rats on day 14 after surgery...
  47. Abbott W, Mellor A, Edwards Y, Feizi T. Soluble bovine galactose-binding lectin. cDNA cloning reveals the complete amino acid sequence and an antigenic relationship with the major encephalitogenic domain of myelin basic protein. Biochem J. 1989;259:283-90 pubmed
    ..In myelin basic protein the tetrapeptide is a part of the major domain previously shown to be responsible for the induction of experimental allergic encephalomyelitis. ..
  48. Ee L, McCannell K, Tang Y, Fernandes N, Hardy W, Green M, et al. An Embryonic Stem Cell-Specific NuRD Complex Functions through Interaction with WDR5. Stem Cell Reports. 2017;8:1488-1496 pubmed publisher
    ..The NuRD subunit MBD3 is essential for targeting and assembly of a functional NuRD complex as well as embryonic stem cell (ESC) ..
  49. Cismasiu V, Adamo K, Gecewicz J, Duque J, Lin Q, Avram D. BCL11B functionally associates with the NuRD complex in T lymphocytes to repress targeted promoter. Oncogene. 2005;24:6753-64 pubmed
    ..Taken together, these data support the hypothesis that the NuRD complex mediates transcriptional repression function of BCL11B. ..
  50. Adenuga D, Rahman I. Protein kinase CK2-mediated phosphorylation of HDAC2 regulates co-repressor formation, deacetylase activity and acetylation of HDAC2 by cigarette smoke and aldehydes. Arch Biochem Biophys. 2010;498:62-73 pubmed publisher
    ..Thus, phospho-acetylation of HDAC2 negatively regulates its deacetylase activity which has implications in steroid resistance in chronic inflammatory conditions. ..
  51. Walavalkar N, Gordon N, Williams D. Unique features of the anti-parallel, heterodimeric coiled-coil interaction between methyl-cytosine binding domain 2 (MBD2) homologues and GATA zinc finger domain containing 2A (GATAD2A/p66?). J Biol Chem. 2013;288:3419-27 pubmed publisher
    ..specificity and high affinity binding, we carried out biophysical analyses of MBD2 and the related homologues MBD3, MBD3-like protein 1 (MBD3L1), and MBD3-like protein 2 (MBD3L2) as well as specific mutations that modify charge-..
  52. Nakamura T, Mori T, Tada S, Krajewski W, Rozovskaia T, Wassell R, et al. ALL-1 is a histone methyltransferase that assembles a supercomplex of proteins involved in transcriptional regulation. Mol Cell. 2002;10:1119-28 pubmed
    ..In parallel, H3-K4 is methylated, and histones H3 and H4 are acetylated at this promoter. ..
  53. Spensberger D, Vermeulen M, Le Guezennec X, Beekman R, van Hoven A, Bindels E, et al. Myeloid transforming protein Evi1 interacts with methyl-CpG binding domain protein 3 and inhibits in vitro histone deacetylation by Mbd3/Mi-2/NuRD. Biochemistry. 2008;47:6418-26 pubmed publisher
    ..We show that interaction of Evi1 with Mbd3 requires 40 amino acids that are adjacent and downstream of the methyl binding domain (MBD)...
  54. Shimbo T, Du Y, Grimm S, Dhasarathy A, Mav D, Shah R, et al. MBD3 localizes at promoters, gene bodies and enhancers of active genes. PLoS Genet. 2013;9:e1004028 pubmed publisher
    ..Here, we provide location analysis of endogenous MBD3, a component of NuRD complex, in two human breast cancer cell lines (MCF-7 and MDA-MB-231) using two independent ..
  55. Loughran S, Comoglio F, Hamey F, Giustacchini A, Errami Y, Earp E, et al. Mbd3/NuRD controls lymphoid cell fate and inhibits tumorigenesis by repressing a B cell transcriptional program. J Exp Med. 2017;214:3085-3104 pubmed publisher
    ..Here we show that the Mbd3/nucleosome remodeling and deacetylation (NuRD) chromatin remodeling complex opposes this transcriptional pioneering ..
  56. Humphrey G, Wang Y, Russanova V, Hirai T, Qin J, Nakatani Y, et al. Stable histone deacetylase complexes distinguished by the presence of SANT domain proteins CoREST/kiaa0071 and Mta-L1. J Biol Chem. 2001;276:6817-24 pubmed
    ..Complex cII (15 S) contained >/=15 proteins, including CHD3/4 (Mi-2), Mta-L1, RbAp48/46, and MBD3, characteristic of vertebrate nucleosome-remodeling complexes...
  57. Brown S, Szyf M. Epigenetic programming of the rRNA promoter by MBD3. Mol Cell Biol. 2007;27:4938-52 pubmed
    ..Here, we propose a role for the methyl-CpG binding domain protein MBD3 in epigenetically maintaining active rRNA promoters...
  58. Kloet S, Baymaz H, Makowski M, Groenewold V, Jansen P, Berendsen M, et al. Towards elucidating the stability, dynamics and architecture of the nucleosome remodeling and deacetylase complex by using quantitative interaction proteomics. FEBS J. 2015;282:1774-85 pubmed publisher
    ..Altogether, these findings extend our understanding of MBD3-NuRD structure and stability...
  59. Cui J, Duan B, Zhao X, Chen Y, Sun S, Deng W, et al. MBD3 mediates epigenetic regulation on EPAS1 promoter in cancer. Tumour Biol. 2016;37:13455-13467 pubmed
    ..Methylated CpG binding protein 3 (MBD3) contributes to the intricate regulatory mechanism...
  60. Feng Q, Zhang Y. The MeCP1 complex represses transcription through preferential binding, remodeling, and deacetylating methylated nucleosomes. Genes Dev. 2001;15:827-32 pubmed
    ..Thus, our study defines the MeCP1 complex, and provides biochemical evidence linking nucleosome remodeling and histone deacetylation to methylated gene silencing. ..
  61. Sun J, Chen H, Davie J. Differential distribution of unmodified and phosphorylated histone deacetylase 2 in chromatin. J Biol Chem. 2007;282:33227-36 pubmed
    ..We provide evidence that unmodified HDAC2 is associated with the coding region of transcribed genes, whereas phosphorylated HDAC2 is primarily recruited to promoters. ..
  62. Bachmann N, Spengler S, Binder G, Eggermann T. MBD3 mutations are not responsible for ICR1 hypomethylation in Silver-Russell syndrome. Eur J Med Genet. 2010;53:23-4 pubmed publisher
    ..Based on the recent findings that a reduced level of the methyl-CpG binding protein 3 (Mbd3) in mice results in a specific hypomethylation of the ICR1 and in a smaller size of embryos we hypothesized that ..
  63. Aguilera C, Nakagawa K, Sancho R, Chakraborty A, Hendrich B, Behrens A. c-Jun N-terminal phosphorylation antagonises recruitment of the Mbd3/NuRD repressor complex. Nature. 2011;469:231-5 pubmed publisher
    ..Here we show that unphosphorylated, but not N-terminally phosphorylated c-Jun, interacts with Mbd3 and thereby recruits the nucleosome remodelling and histone deacetylation (NuRD) repressor complex...
  64. Cramer J, Scarsdale J, Walavalkar N, Buchwald W, Ginder G, Williams D. Probing the dynamic distribution of bound states for methylcytosine-binding domains on DNA. J Biol Chem. 2014;289:1294-302 pubmed publisher
    Although highly homologous to other methylcytosine-binding domain (MBD) proteins, MBD3 does not selectively bind methylated DNA, and thus the functional role of MBD3 remains in question...
  65. Cui Y, Irudayaraj J. Dissecting the behavior and function of MBD3 in DNA methylation homeostasis by single-molecule spectroscopy and microscopy. Nucleic Acids Res. 2015;43:3046-55 pubmed publisher
    ..relies on an intricate regulatory network with a possible contribution from methyl-CpG-binding domain protein 3 (MBD3)...
  66. Fujita N, Jaye D, Geigerman C, Akyildiz A, Mooney M, Boss J, et al. MTA3 and the Mi-2/NuRD complex regulate cell fate during B lymphocyte differentiation. Cell. 2004;119:75-86 pubmed
  67. Li Q, Shi L, Gui B, Yu W, Wang J, Zhang D, et al. Binding of the JmjC demethylase JARID1B to LSD1/NuRD suppresses angiogenesis and metastasis in breast cancer cells by repressing chemokine CCL14. Cancer Res. 2011;71:6899-908 pubmed publisher
    ..Our findings indicate that CCL14 is a critical mediator of the JARID1B/LSD1/NuRD complex in regulation of angiogenesis and metastasis in breast cancer, identifying a novel potential therapeutic target for breast cancer intervention. ..
  68. Pontes T, Chen E, Gigek C, Calcagno D, Wisnieski F, Leal M, et al. Reduced mRNA expression levels of MBD2 and MBD3 in gastric carcinogenesis. Tumour Biol. 2014;35:3447-53 pubmed publisher
    ..To evaluate the role of MBD2 and MBD3 in gastric carcinogenesis and the possible association with clinicopathological characteristics, we assessed the ..
  69. Schlegel J, Güneysu S, Mennel H. Expression of the genes of methyl-binding domain proteins in human gliomas. Oncol Rep. 2002;9:393-5 pubmed
    ..We found expression of MBDs including MBD1, MBD2, MBD3 and MBD4/MED1 in all glioma cell lines and glioma biopsies...
  70. Fukushige S, Kondo E, Gu Z, Suzuki H, Horii A. RET finger protein enhances MBD2- and MBD4-dependent transcriptional repression. Biochem Biophys Res Commun. 2006;351:85-92 pubmed
    ..Furthermore, RFP forms a protein complex not only with MBD4 but also with MBD2 or MBD3 and was shown to enhance transcriptional repression through MBD2...
  71. Opsahl J, Hjørnevik L, Bull V, Fismen L, Frøyset A, Gromyko D, et al. Increased interaction between DJ-1 and the Mi-2/ nucleosome remodelling and deacetylase complex during cellular stress. Proteomics. 2010;10:1494-504 pubmed publisher
    ..The observed interaction between DJ-1 and the NuRD complex may give new clues to how DJ-1 can protect cells from p53-dependent cell death. ..
  72. Mahajan M, Narlikar G, Boyapaty G, Kingston R, Weissman S. Heterogeneous nuclear ribonucleoprotein C1/C2, MeCP1, and SWI/SNF form a chromatin remodeling complex at the beta-globin locus control region. Proc Natl Acad Sci U S A. 2005;102:15012-7 pubmed
    ..Independently of the initiation of transcription, LARC becomes associated with beta-like globin promoters. ..
  73. Rais Y, Zviran A, Geula S, Gafni O, Chomsky E, Viukov S, et al. Deterministic direct reprogramming of somatic cells to pluripotency. Nature. 2013;502:65-70 pubmed publisher
    ..Here we show that depleting Mbd3, a core member of the Mbd3/NuRD (nucleosome remodelling and deacetylation) repressor complex, together with OSKM ..
  74. Cui Y, Li J, Weng L, Wirbisky S, Freeman J, Liu J, et al. Regulatory landscape and clinical implication of MBD3 in human malignant glioma. Oncotarget. 2016;7:81698-81714 pubmed publisher
    In this article we inspect the roles and functions of the methyl-CpG-binding domain protein 3 (MBD3) in human malignant glioma, to assess its potential as an epigenetic biomarker for prognosis...
  75. Carter C, Lin C, Liu C, Yang L, Liu Z. Phosphorylated p68 RNA helicase activates Snail1 transcription by promoting HDAC1 dissociation from the Snail1 promoter. Oncogene. 2010;29:5427-36 pubmed publisher
    ..Our results showed that p68 interacted with the nuclear remodeling and deacetylation complex MBD3:Mi-2/NuRD...
  76. Li X, Foley E, Kawashima S, Molloy K, Li Y, Chait B, et al. Examining post-translational modification-mediated protein-protein interactions using a chemical proteomics approach. Protein Sci. 2013;22:287-95 pubmed publisher
    ..Together, our findings indicate the CLASPI approach can be broadly applied to profile protein-protein interactions mediated by PTMs. ..
  77. Quan J, Adelmant G, Marto J, Look A, Yusufzai T. The chromatin remodeling factor CHD5 is a transcriptional repressor of WEE1. PLoS ONE. 2014;9:e108066 pubmed publisher
    ..Our study shows that CHD5 is a NuRD-associated transcriptional repressor and identifies WEE1 as one of the CHD5-regulated genes that may link CHD5 to tumor suppression. ..
  78. Sakai H, Urano T, Ookata K, Kim M, Hirai Y, Saito M, et al. MBD3 and HDAC1, two components of the NuRD complex, are localized at Aurora-A-positive centrosomes in M phase. J Biol Chem. 2002;277:48714-23 pubmed
    b>MBD3, a component of the histone deacetylase NuRD complex, contains the methyl-CpG-binding domain (MBD), yet does not possess appreciable mCpG-specific binding activity...
  79. Wang Y, Zhang H, Chen Y, Sun Y, Yang F, Yu W, et al. LSD1 is a subunit of the NuRD complex and targets the metastasis programs in breast cancer. Cell. 2009;138:660-72 pubmed publisher
    ..By enlisting LSD1, the NuRD complex expands its chromatin remodeling capacity to include ATPase, histone deacetylase, and histone demethylase. ..
  80. Wade P, Gegonne A, Jones P, Ballestar E, Aubry F, Wolffe A. Mi-2 complex couples DNA methylation to chromatin remodelling and histone deacetylation. Nat Genet. 1999;23:62-6 pubmed
    ..Our data substantiate the mechanistic link between DNA methylation, histone deacetylation and transcriptional silencing. ..
  81. Tatematsu K, Yamazaki T, Ishikawa F. MBD2-MBD3 complex binds to hemi-methylated DNA and forms a complex containing DNMT1 at the replication foci in late S phase. Genes Cells. 2000;5:677-88 pubmed
    ..However, the function of another member of this family, MBD3, remained elusive. It was shown that MBD2 and MBD3 form homo- and hetero-dimers (or multimers) in vitro and in vivo...
  82. Shi Y, Downes M, Xie W, Kao H, Ordentlich P, Tsai C, et al. Sharp, an inducible cofactor that integrates nuclear receptor repression and activation. Genes Dev. 2001;15:1140-51 pubmed
    ..Surprisingly, the expression of SHARP is itself steroid inducible, suggesting a simple feedback mechanism for attenuation of the hormonal response. ..
  83. Jin S, Jiang C, Rauch T, Li H, Pfeifer G. MBD3L2 interacts with MBD3 and components of the NuRD complex and can oppose MBD2-MeCP1-mediated methylation silencing. J Biol Chem. 2005;280:12700-9 pubmed
    MBD2 and MBD3 are two proteins that contain methyl-CpG binding domains and have a transcriptional repression function...
  84. Gong Z, Brackertz M, Renkawitz R. SUMO modification enhances p66-mediated transcriptional repression of the Mi-2/NuRD complex. Mol Cell Biol. 2006;26:4519-28 pubmed
    ..are two potent transcriptional repressors that interact with the methyl-CpG-binding domain proteins MBD2 and MBD3. An analysis of the molecular mechanisms mediating repression resulted in the identification of two major ..
  85. Brown S, Suderman M, Hallett M, Szyf M. DNA demethylation induced by the methyl-CpG-binding domain protein MBD3. Gene. 2008;420:99-106 pubmed publisher
    The methyl-CpG binding domain protein MBD3 has been shown to be essential for embryonic development and differentiation, and to act by suppressing gene expression through the recruitment of co-repressor complexes...
  86. Choi W, Yoon J, Kim M, Koh D, Licht J, Kim K, et al. Promyelocytic leukemia zinc finger-retinoic acid receptor ? (PLZF-RAR?), an oncogenic transcriptional repressor of cyclin-dependent kinase inhibitor 1A (p21WAF/CDKN1A) and tumor protein p53 (TP53) genes. J Biol Chem. 2014;289:18641-56 pubmed publisher
    ..PLZF-RAR? also interacts with the MBD3-NuRD complex, leading to epigenetic silencing of CDKN1A through DNA methylation...
  87. Li R, He Q, Han S, Zhang M, Liu J, Su M, et al. MBD3 inhibits formation of liver cancer stem cells. Oncotarget. 2017;8:6067-6078 pubmed publisher
    ..We showed that suppression of methyl-CpG binding domain protein 3 (MBD3), a core subunit of the NuRD repressor complex, together with OSKM transduction, induces conversion of liver cancer ..
  88. Xu M, He J, Li J, Feng W, Zhou H, Wei H, et al. Methyl-CpG-binding domain 3 inhibits epithelial-mesenchymal transition in pancreatic cancer cells via TGF-β/Smad signalling. Br J Cancer. 2017;116:91-99 pubmed publisher
    Methyl-CpG-binding domain 3 (MBD3) is an aberrant expression in human malignancies. However, the role of MBD3 in pancreatic cancer progression remains to be clarified...
  89. Jiang C, Jin S, Pfeifer G. MBD3L1 is a transcriptional repressor that interacts with methyl-CpG-binding protein 2 (MBD2) and components of the NuRD complex. J Biol Chem. 2004;279:52456-64 pubmed
    Methyl-CpG-binding domain proteins 2 and 3 (MBD2 and MBD3) are transcriptional repressors that contain methyl-CpG binding domains and are components of a CpG-methylated DNA binding complex named MeCP1...
  90. Zhu P, Baek S, Bourk E, Ohgi K, Garcia Bassets I, Sanjo H, et al. Macrophage/cancer cell interactions mediate hormone resistance by a nuclear receptor derepression pathway. Cell. 2006;124:615-29 pubmed
  91. Sillibourne J, Delaval B, Redick S, Sinha M, Doxsey S. Chromatin remodeling proteins interact with pericentrin to regulate centrosome integrity. Mol Biol Cell. 2007;18:3667-80 pubmed
    ..We conclude that pericentrin forms complexes with CHD3 and CHD4, but a distinct CHD3-pericentrin complex is required for centrosomal anchoring of pericentrin/gamma-tubulin and for centrosome integrity. ..
  92. Noh E, Lim D, Lee J. A novel role for methyl CpG-binding domain protein 3, a component of the histone deacetylase complex, in regulation of cell cycle progression and cell death. Biochem Biophys Res Commun. 2009;378:332-7 pubmed publisher
    ..Here, we investigated the molecular mechanisms by which methyl CpG-binding domain protein 3 (MBD3), a component of the HDAC complex, modulates these processes via its functional interplay with HDAC...