Gene Symbol: MAP1LC3B
Description: microtubule associated protein 1 light chain 3 beta
Alias: ATG8F, LC3B, MAP1A/1BLC3, MAP1LC3B-a, microtubule-associated proteins 1A/1B light chain 3B, MAP1 light chain 3-like protein 2, MAP1A/MAP1B LC3 B, MAP1A/MAP1B light chain 3 B, autophagy-related ubiquitin-like modifier LC3 B
Species: human
Products:     MAP1LC3B

Top Publications

  1. Othman E, Kaur G, Mutee A, Muhammad T, Tan M. Immunohistochemical expression of MAP1LC3A and MAP1LC3B protein in breast carcinoma tissues. J Clin Lab Anal. 2009;23:249-58 pubmed publisher
    ..The aims of this study were to determine the MAP1LC3A and MAP1LC3B protein expression in both normal and cancer breast tissues and to determine the relationship between the ..
  2. Pankiv S, Clausen T, Lamark T, Brech A, Bruun J, Outzen H, et al. p62/SQSTM1 binds directly to Atg8/LC3 to facilitate degradation of ubiquitinated protein aggregates by autophagy. J Biol Chem. 2007;282:24131-45 pubmed
    ..In fact, p62 bodies and these structures are indistinguishable. Taken together, our results clearly suggest that p62 is required both for the formation and the degradation of polyubiquitin-containing bodies by autophagy. ..
  3. Chen S, Jiang Y, Huang L, Zhou R, Yu K, Liu Y, et al. The residual tumor autophagy marker LC3B serves as a prognostic marker in local advanced breast cancer after neoadjuvant chemotherapy. Clin Cancer Res. 2013;19:6853-62 pubmed publisher sought to investigate the prognostic value of the autophagy marker microtubule-associated protein chain 3B (LC3B) in patients with residual tumors after neoadjuvant chemotherapy (NCT) for locally advanced breast cancer (LABC)...
  4. Dagda R, Cherra S, Kulich S, Tandon A, Park D, Chu C. Loss of PINK1 function promotes mitophagy through effects on oxidative stress and mitochondrial fission. J Biol Chem. 2009;284:13843-55 pubmed publisher
    ..Furthermore, PINK1 and Parkin may cooperate through different mechanisms to maintain mitochondrial homeostasis. ..
  5. Colecchia D, Strambi A, Sanzone S, Iavarone C, Rossi M, Dall Armi C, et al. MAPK15/ERK8 stimulates autophagy by interacting with LC3 and GABARAP proteins. Autophagy. 2012;8:1724-40 pubmed publisher
    ..We discovered ATG8-like proteins (MAP1LC3B, GABARAP and GABARAPL1) as novel interactors of MAPK15/ERK8, a MAP kinase involved in cell proliferation and ..
  6. Tanida I, Ueno T, Kominami E. Human light chain 3/MAP1LC3B is cleaved at its carboxyl-terminal Met121 to expose Gly120 for lipidation and targeting to autophagosomal membranes. J Biol Chem. 2004;279:47704-10 pubmed
    Human light chain 3/MAP1LC3B, an autophagosomal ortholog of yeast Atg8, is conjugated to phospholipid (PL) via ubiquitylation-like reactions mediated by human Atg7 and Atg3...
  7. Itoh T, Kanno E, Uemura T, Waguri S, Fukuda M. OATL1, a novel autophagosome-resident Rab33B-GAP, regulates autophagosomal maturation. J Cell Biol. 2011;192:839-53 pubmed publisher
  8. Sou Y, Tanida I, Komatsu M, Ueno T, Kominami E. Phosphatidylserine in addition to phosphatidylethanolamine is an in vitro target of the mammalian Atg8 modifiers, LC3, GABARAP, and GATE-16. J Biol Chem. 2006;281:3017-24 pubmed
    ..The discrepancy between in vitro and in vivo reactions suggested that there may be selective factor(s) involved in the endogenous LC3 conjugation system. ..
  9. Satoo K, Noda N, Kumeta H, Fujioka Y, Mizushima N, Ohsumi Y, et al. The structure of Atg4B-LC3 complex reveals the mechanism of LC3 processing and delipidation during autophagy. EMBO J. 2009;28:1341-50 pubmed publisher

More Information

Publications106 found, 100 shown here

  1. Novak I, Kirkin V, McEwan D, Zhang J, Wild P, Rozenknop A, et al. Nix is a selective autophagy receptor for mitochondrial clearance. EMBO Rep. 2010;11:45-51 pubmed publisher
    ..Thus, Nix functions as an autophagy receptor, which mediates mitochondrial clearance after mitochondrial damage and during erythrocyte differentiation. ..
  2. Huang X, Bai H, Chen L, Li B, Lu Y. Reduced expression of LC3B-II and Beclin 1 in glioblastoma multiforme indicates a down-regulated autophagic capacity that relates to the progression of astrocytic tumors. J Clin Neurosci. 2010;17:1515-9 pubmed publisher
    The aim of this study was to investigate the expression of microtubule-associated protein 1 light chain 3B (LC3B) and the autophagy-related gene Beclin 1 in astrocytic tumors and to analyze their expression profiles with respect to the ..
  3. Sancho A, Duran J, Garcia Espana A, Mauvezin C, Alemu E, Lamark T, et al. DOR/Tp53inp2 and Tp53inp1 constitute a metazoan gene family encoding dual regulators of autophagy and transcription. PLoS ONE. 2012;7:e34034 pubmed publisher
    ..Taken together, our data reveal DOR and TP53INP1 as dual regulators of transcription and autophagy, and identify two conserved regions in the DOR family that concentrate multiple functions crucial for autophagy and transcription. ..
  4. Liu L, Feng D, Chen G, Chen M, Zheng Q, Song P, et al. Mitochondrial outer-membrane protein FUNDC1 mediates hypoxia-induced mitophagy in mammalian cells. Nat Cell Biol. 2012;14:177-85 pubmed publisher
    ..Mechanistic studies further revealed that hypoxia induced dephosphorylation of FUNDC1 and enhanced its interaction with LC3 for selective mitophagy. Our findings thus offer insights into mitochondrial quality control in mammalian cells. ..
  5. Kabeya Y, Mizushima N, Ueno T, Yamamoto A, Kirisako T, Noda T, et al. LC3, a mammalian homologue of yeast Apg8p, is localized in autophagosome membranes after processing. EMBO J. 2000;19:5720-8 pubmed
    ..The amount of LC3-II is correlated with the extent of autophagosome formation. LC3-II is the first mammalian protein identified that specifically associates with autophagosome membranes. ..
  6. Tanida I, Ueno T, Kominami E. LC3 conjugation system in mammalian autophagy. Int J Biochem Cell Biol. 2004;36:2503-18 pubmed
    ..We also highlight recent findings regarding the pathobiology of LC3-modification, including its role in microbial infection, cancer and neuromuscular diseases. ..
  7. Tanida I, Tanida Miyake E, Komatsu M, Ueno T, Kominami E. Human Apg3p/Aut1p homologue is an authentic E2 enzyme for multiple substrates, GATE-16, GABARAP, and MAP-LC3, and facilitates the conjugation of hApg12p to hApg5p. J Biol Chem. 2002;277:13739-44 pubmed
    ..Furthermore, hApg3p coimmunoprecipitates with hApg12p, and the overexpression of hApg3p facilitates the formation of the GFPhApg12p.thApg5p conjugate, suggesting that hApg3p cross-talks with the hApg12p conjugation system. ..
  8. Rouschop K, van den Beucken T, Dubois L, Niessen H, Bussink J, Savelkouls K, et al. The unfolded protein response protects human tumor cells during hypoxia through regulation of the autophagy genes MAP1LC3B and ATG5. J Clin Invest. 2010;120:127-41 pubmed publisher
    ..increased transcription of the essential autophagy genes microtubule-associated protein 1 light chain 3beta (MAP1LC3B) and autophagy-related gene 5 (ATG5) through the transcription factors ATF4 and CHOP, respectively, which are ..
  9. He H, Dang Y, Dai F, Guo Z, Wu J, She X, et al. Post-translational modifications of three members of the human MAP1LC3 family and detection of a novel type of modification for MAP1LC3B. J Biol Chem. 2003;278:29278-87 pubmed
    ..we report the identification and characterization of three human orthologs of the rat Map1LC3, named MAP1LC3A, MAP1LC3B, and MAP1LC3C...
  10. Komatsu M, Waguri S, Koike M, Sou Y, Ueno T, Hara T, et al. Homeostatic levels of p62 control cytoplasmic inclusion body formation in autophagy-deficient mice. Cell. 2007;131:1149-63 pubmed
  11. Kirkin V, Lamark T, Sou Y, Bjørkøy G, Nunn J, Bruun J, et al. A role for NBR1 in autophagosomal degradation of ubiquitinated substrates. Mol Cell. 2009;33:505-16 pubmed publisher
    ..We propose that NBR1 and p62 act as receptors for selective autophagosomal degradation of ubiquitinated targets. ..
  12. Espert L, Varbanov M, Robert Hebmann V, Sagnier S, Robbins I, Sanchez F, et al. Differential role of autophagy in CD4 T cells and macrophages during X4 and R5 HIV-1 infection. PLoS ONE. 2009;4:e5787 pubmed publisher
    ..Taken together, our data suggest that autophagy plays a complex, but essential, role in HIV pathology by regulating both viral replication and the fate of the target cells. ..
  13. Wild P, Farhan H, McEwan D, Wagner S, Rogov V, Brady N, et al. Phosphorylation of the autophagy receptor optineurin restricts Salmonella growth. Science. 2011;333:228-33 pubmed publisher
    ..We propose that phosphorylation of autophagy receptors might be a general mechanism for regulation of cargo-selective autophagy. ..
  14. Popovic D, Akutsu M, Novak I, Harper J, Behrends C, Dikic I. Rab GTPase-activating proteins in autophagy: regulation of endocytic and autophagy pathways by direct binding to human ATG8 modifiers. Mol Cell Biol. 2012;32:1733-44 pubmed publisher
    ..We propose that LC3-interacting Rab GAPs are implicated in the reprogramming of the endocytic trafficking events under starvation-induced autophagy. ..
  15. Alemu E, Lamark T, Torgersen K, Birgisdottir A, Larsen K, Jain A, et al. ATG8 family proteins act as scaffolds for assembly of the ULK complex: sequence requirements for LC3-interacting region (LIR) motifs. J Biol Chem. 2012;287:39275-90 pubmed publisher
    ..Importantly, the LIR motif is required for starvation-induced association of ULK1 with autophagosomes. Our data suggest that ATG8 proteins act as scaffolds for assembly of the ULK complex at the phagophore. ..
  16. Ko Y, Cho Y, Won H, Jeon E, An H, Hong S, et al. Prognostic significance of autophagy-related protein expression in resected pancreatic ductal adenocarcinoma. Pancreas. 2013;42:829-35 pubmed publisher
    ..3% (36/73) for Atg5, 63.9% (46/72) for Ambra1, 47.9% (35/73) for beclin-1, 83.3% (60/72) for LC3B, and 69.9% (51/73) for Bif-1...
  17. So K, Lee B, Oh S. The critical role of autophagy in cadmium-induced immunosuppression regulated by endoplasmic reticulum stress-mediated calpain activation in RAW264.7 mouse monocytes. Toxicology. 2018;393:15-25 pubmed publisher
    ..Furthermore, Cd induced autophagy, as demonstrated by microtubule-associated protein 1 light chain 3B (LC3B) plasmid DNA transfection and its conversion from LC3-I to the LC3-II form by autophagy inhibitors, via AMP-..
  18. Yang Q, Sun G, Yin H, Li H, Cao Z, Wang J, et al. PINK1 Protects Auditory Hair Cells and Spiral Ganglion Neurons from Cisplatin-induced Ototoxicity via Inducing Autophagy and Inhibiting JNK Signaling Pathway. Free Radic Biol Med. 2018;120:342-355 pubmed publisher
    ..The expressions of PINK1, LC3B, cleaved-caspase 3 and p-JNK were measured by Western blotting...
  19. Chen Y, Shi S, Wang H, Li N, Su J, Chou G, et al. A Homogeneous Polysaccharide from Fructus Schisandra chinensis (Turz.) Baill Induces Mitochondrial Apoptosis through the Hsp90/AKT Signalling Pathway in HepG2 Cells. Int J Mol Sci. 2016;17: pubmed publisher
    ..c (Cyt C); tumor protein 53 (p53); survivin; sequestosome 1 (p62); microtubule-associated protein 1 light chain-3B (LC3B); mitogen-activated protein kinase p38 (p38); extracellular regulated protein kinases (ERK); c-Jun N-terminal ..
  20. Frank B, Marcu A, de Oliveira Almeida Petersen A, Weber H, Stigloher C, Mottram J, et al. Autophagic digestion of Leishmania major by host macrophages is associated with differential expression of BNIP3, CTSE, and the miRNAs miR-101c, miR-129, and miR-210. Parasit Vectors. 2015;8:404 pubmed publisher
    ..cathepsin E (CTSE), mechanistic target of rapamycin (MTOR), microtubule-associated proteins 1A/1B light chain 3B (LC3B), and ubiquitin (UB) were investigated through western blot analyses...
  21. Pantazi E, Zaouali M, Bejaoui M, Folch Puy E, Ben Abdennebi H, Varela A, et al. Sirtuin 1 in rat orthotopic liver transplantation: an IGL-1 preservation solution approach. World J Gastroenterol. 2015;21:1765-74 pubmed publisher
    ..activated protein kinase (AMPK), p-mTOR, p-p70S6K (direct substrate of mTOR), autophagy parameters (beclin-1, LC3B) and MAP kinases (p-p38 and p-ERK) were determined by Western blot...
  22. Felszeghy S, Viiri J, Paterno J, Hyttinen J, Koskela A, Chen M, et al. Loss of NRF-2 and PGC-1α genes leads to retinal pigment epithelium damage resembling dry age-related macular degeneration. Redox Biol. 2019;20:1-12 pubmed publisher
    ..Moreover, levels of protein ubiquitination and autophagy markers p62/SQSTM1 (sequestosome 1), Beclin-1 and LC3B (microtubule associated protein 1 light chain 3 beta) were significantly increased together with the Iba-1 (ionized ..
  23. Wang Y, Twu Y, Wang C, Lin F, Lee C, Liao Y. Niemann-Pick Type C2 Protein Regulates Free Cholesterol Accumulation and Influences Hepatic Stellate Cell Proliferation and Mitochondrial Respiration Function. Int J Mol Sci. 2018;19: pubmed publisher
    ..Although NPC2 expression did not affect caspase-related apoptosis, the autophagy marker light chain 3β (LC3B) was decreased in NPC2 knockdown, and free cholesterol accumulated in the HSCs...
  24. Wang B, Cao P, Wang Z, Li Z, Wang Z, Ma J, et al. Interferon-γ-induced insufficient autophagy contributes to p62-dependent apoptosis of epithelial cells in chronic rhinosinusitis with nasal polyps. Allergy. 2017;72:1384-1397 pubmed publisher
    ..The expression of autophagic proteins [microtubule-associated protein 1 light chain 3B (LC3B)-II, autophagy-related proteins (Atg), and Beclin 1], substrate proteins (p62 and ubiquitinated proteins), and ..
  25. Huang D, Bao L. Mycobacterium tuberculosis EspB protein suppresses interferon-γ-induced autophagy in murine macrophages. J Microbiol Immunol Infect. 2016;49:859-865 pubmed publisher
    ..Levels of autophagic markers, including Microtubule-associated protein 1 light chain 3 beta (LC3B-I and -II), phosphorylated signal transducer and activator of transcription (STAT)1 and total STAT1 in ANA-1 cells ..
  26. O Donnell J, Jackson J, Robinson M. Transient Oxygen/Glucose Deprivation Causes a Delayed Loss of Mitochondria and Increases Spontaneous Calcium Signaling in Astrocytic Processes. J Neurosci. 2016;36:7109-27 pubmed publisher
    ..mitochondria from astrocytic processes and increased colocalization of mitochondria with the autophagosome marker LC3B. The losses of neurons in area CA1 and mitochondria in astrocytic processes were blocked by ionotropic glutamate ..
  27. Dutta D, Chakraborty B, Sarkar A, Chowdhury C, Das P. A potent betulinic acid analogue ascertains an antagonistic mechanism between autophagy and proteasomal degradation pathway in HT-29 cells. BMC Cancer. 2016;16:23 pubmed publisher
    ..2c was found to alter expression levels of different autophagic proteins like Beclin-1, Atg 5, Atg 7, Atg 5-Atg 12, LC3B and autophagic adapter protein, p62...
  28. McManus S, Bisson M, Chamberland R, Roy M, Nazari S, Roux S. Autophagy and 3-Phosphoinositide-Dependent Kinase 1 (PDK1)-Related Kinome in Pagetic Osteoclasts. J Bone Miner Res. 2016;31:1334-43 pubmed publisher
    ..The resistance to apoptosis and the bone resorption were also overcome upon PDK1 inhibition. Studying autophagy by LC3B expression, we found a less inducible autophagy compared with control cells, which was reversed by PDK1 inhibition...
  29. Sotthibundhu A, McDonagh K, von Kriegsheim A, Garcia Munoz A, Klawiter A, Thompson K, et al. Rapamycin regulates autophagy and cell adhesion in induced pluripotent stem cells. Stem Cell Res Ther. 2016;7:166 pubmed
    ..levels of autophagy, including key components of APMK?, ULK1/2, BECLIN-1, ATG13, ATG101, ATG12, ATG3, ATG5, and LC3B. Block of autophagy by bafilomycin induces iPSC death and rapamycin attenuates the bafilomycin effect...
  30. Dong W, Wang R, Ma L, Xu B, Zhang J, Zhao Z, et al. Influence of age-related learning and memory capacity of mice: different effects of a high and low caloric diet. Aging Clin Exp Res. 2016;28:303-11 pubmed publisher
    ..The expression of GFAP was determined by immunofluorescence and western blot. The expression of mTOR, S6K and LC3B in the hippocampus was determined by immunofluorescence...
  31. Xu S, Guo X, Gao X, Xue H, Zhang J, Guo X, et al. Macrophage migration inhibitory factor enhances autophagy by regulating ROCK1 activity and contributes to the escape of dendritic cell surveillance in glioblastoma. Int J Oncol. 2016;49:2105-2115 pubmed publisher
    ..In glioma specimens, MIF expression was significantly correlated with LC3B levels...
  32. Zhang Z, Ren Z, Chen S, Guo X, Liu F, Guo L, et al. ROS generation and JNK activation contribute to 4-methoxy-TEMPO-induced cytotoxicity, autophagy, and DNA damage in HepG2 cells. Arch Toxicol. 2018;92:717-728 pubmed publisher
    ..In addition, 4-methoxy-TEMPO also induced autophagy as demonstrated by the conversion of LC3B-I to II, decreased level of p62, and the appearance of GFP-LC3B punctae...
  33. Wu S, Lu I, Lee S, Kwan A, Chai C, Huang S. Erythropoietin attenuates motor neuron programmed cell death in a burn animal model. PLoS ONE. 2018;13:e0190039 pubmed publisher
    ..In addition, we examined autophagy changes by biomarkers of LC3B and ATG5...
  34. Rogov V, Rozenknop A, Rogova N, Lohr F, Tikole S, Jaravine V, et al. A universal expression tag for structural and functional studies of proteins. Chembiochem. 2012;13:959-63 pubmed publisher
    ..With this new system we have been able to characterize several peptide-protein interactions by ITC and by NMR and CD spectroscopic methods, including the interactions of LIR domains with autophagy modifiers. ..
  35. Contreras A, Mebratu Y, Delgado M, Montaño G, Hu C, Ryter S, et al. Deacetylation of p53 induces autophagy by suppressing Bmf expression. J Cell Biol. 2013;201:427-37 pubmed publisher
    ..Furthermore, autophagy was prominent in cultured bmf(-/-) but not in bmf(+/+) cells. Collectively, these observations show that deacetylation of p53 suppresses Bmf expression and facilitates autophagy. ..
  36. Sudhagar S, Sathya S, Gokulapriya G, Lakshmi B. AKT-p53 axis protect cancer cells from autophagic cell death during nutrition deprivation. Biochem Biophys Res Commun. 2016;471:396-401 pubmed publisher
    ..Silencing of AKT and p53 enhanced autophagy as evidenced by increased acidic vesicular organelles and LC3B II levels, suggesting AKT-p53 to play a significant role in cell survival through regulating autophagy during ..
  37. Altmann C, Hardt S, Fischer C, Heidler J, Lim H, Häussler A, et al. Progranulin overexpression in sensory neurons attenuates neuropathic pain in mice: Role of autophagy. Neurobiol Dis. 2016;96:294-311 pubmed publisher
    ..Nerve injury in vivo caused an accumulation of LC3b-EGFP positive bodies in neurons of the dorsal root ganglia and nerves suggesting an impairment of autophagic flux...
  38. Samuel S, Ghosh S, Majeed Y, Arunachalam G, Emara M, Ding H, et al. Metformin represses glucose starvation induced autophagic response in microvascular endothelial cells and promotes cell death. Biochem Pharmacol. 2017;132:118-132 pubmed publisher evidenced by the significant increase in the levels of ER stress and autophagic markers while accumulation of LC3B stained punctae in the MECs confirmed autophagic activation...
  39. Huang J, Pan W, Ou D, Dai W, Lin Y, Chen Y, et al. LC3B, a Protein That Serves as an Autophagic Marker, Modulates Angiotensin II-induced Myocardial Hypertrophy. J Cardiovasc Pharmacol. 2015;66:576-83 pubmed publisher
    b>LC3B is a marker of autophagic activity, and growing evidence supports its importance in myocardial hypertrophy...
  40. Lee J, Shin J, Shim H, Lee C, Kim D, Kim Y, et al. Autophagy contributes to the chemo-resistance of non-small cell lung cancer in hypoxic conditions. Respir Res. 2015;16:138 pubmed publisher
    ..Hypoxic exposure also induced resistance of cancer cells to cisplatin, and LC3B siRNA restored the sensitivity of cancer cells to chemotherapy...
  41. Dyshlovoy S, Madanchi R, Hauschild J, Otte K, Alsdorf W, Schumacher U, et al. The marine triterpene glycoside frondoside A induces p53-independent apoptosis and inhibits autophagy in urothelial carcinoma cells. BMC Cancer. 2017;17:93 pubmed publisher
    ..Effects on autophagy were studied using LC3B-I/II and SQSTM/p62 as markers. The unpaired Student's t-test was used for comparison of the data sets...
  42. Yamamoto J, Kamata S, Miura A, Nagata T, Kainuma R, Ishii I. Differential adaptive responses to 1- or 2-day fasting in various mouse tissues revealed by quantitative PCR analysis. FEBS Open Bio. 2015;5:357-68 pubmed publisher
    ..using 15 marker primer sets for the activation of ubiquitin-proteasome (Atrogin-1 and MuRF1), autophagy-lysosome (LC3b, p62 and Lamp2), amino acid response (Asns, Trib3, Herpud1, xCT, and Chop), Nrf2-mediated antioxidant (HO-1 and ..
  43. Wang Y, Yan J, Yin F, Li L, Qin Y, Meng C, et al. Role of autophagy in cadmium-induced testicular injury. Hum Exp Toxicol. 2017;36:1039-1048 pubmed publisher
    ..showed that levels of the autophagy-related proteins Beclin1 and microtubule-associated protein 1 light chain 3B (LC3B) increased with increased cadmium exposure...
  44. Zhao H, Xi H, Wei B, Cai A, Wang T, Wang Y, et al. Expression of decorin in intestinal tissues of mice with inflammatory bowel disease and its correlation with autophagy. Exp Ther Med. 2016;12:3885-3892 pubmed publisher
    ..The expression of DCN as well as the autophagy-associated proteins beclin 1 and LC3B, was increased in the intestinal tissues of the IBD mice...
  45. Ahn M, Yoon J. Histone deacetylase 7 silencing induces apoptosis and autophagy in salivary mucoepidermoid carcinoma cells. J Oral Pathol Med. 2017;46:276-283 pubmed publisher
    ..HDAC7 silencing induced an increase in autophagic markers, number of acidic vesicular organelles, and LC3B II levels, and decrease in p62 levels. HDAC7 siRNAs reduced the activation of ERK...
  46. Wu Z, Zhao J, Qiu M, Mi Z, Meng M, Guo Y, et al. CRISPR/Cas9 Mediated GFP Knock-in at the MAP1LC3B Locus in 293FT Cells Is Better for Bona Fide Monitoring Cellular Autophagy. Biotechnol J. 2018;13:e1700674 pubmed publisher
    ..Ectopically expressed fluorescent-tagged microtubule-associated protein light chain 3B (MAP1LC3B, LC3) is the most widely used reporter for monitoring autophagy activity thus far...
  47. Liu Z, Tang J, Chen Z, Zhang H, Wang H, Yang J, et al. The novel mTORC1/2 dual inhibitor INK128 enhances radiosensitivity of breast cancer cell line MCF-7. Int J Oncol. 2016;49:1039-45 pubmed publisher
    ..Furthermore, INK128 plus radiation downregulated p-Chk2, p21 and upregulated cleaved PARP, LC3B expression...
  48. Awan F, Obaid A, Ikram A, Janjua H. Mutation-Structure-Function Relationship Based Integrated Strategy Reveals the Potential Impact of Deleterious Missense Mutations in Autophagy Related Proteins on Hepatocellular Carcinoma (HCC): A Comprehensive Informatics Approach. Int J Mol Sci. 2017;18: pubmed publisher
    ..that are highly damaging to the structure as well as function of autophagy related proteins including LC3A, LC3B, BECN1 and SCD1...
  49. Cai S, Shi G, Cheng H, Zeng Y, Li G, Zhang M, et al. Exosomal miR-7 Mediates Bystander Autophagy in Lung after Focal Brain Irradiation in Mice. Int J Biol Sci. 2017;13:1287-1296 pubmed publisher
    ..Meanwhile, Dil-stained exosomes released from 10 Gy or sham irradiated astrocytes were injected into LC3B-GFP mice via the tail vein. Lung tissues were then extracted for western blotting and qRT-PCR...
  50. Peterse E, Niessen B, Addie R, de Jong Y, Cleven A, Kruisselbrink A, et al. Targeting glutaminolysis in chondrosarcoma in context of the IDH1/2 mutation. Br J Cancer. 2018;118:1074-1083 pubmed publisher
    ..Metformin and phenformin decreased mTOR activity in chondrosarcoma cells, and metformin decreased LC3B-II levels, which is counteracted by chloroquine...
  51. Du J, Wang L, Wang Y, Shen J, Pan C, Meng Y, et al. Autophagy and apoptosis induced by Chinese giant salamander (Andrias davidianus) iridovirus (CGSIV). Vet Microbiol. 2016;195:87-95 pubmed publisher
    ..The LC3B protein displayed uneven distribution by Immunohistochemistry and the level mRNA of Atg5 was higher in these ..
  52. Muzes G, Kiss A, Tulassay Z, Sipos F. Cell-free DNA-induced alteration of autophagy response and TLR9-signaling: Their relation to amelioration of DSS-colitis. Comp Immunol Microbiol Infect Dis. 2017;52:48-57 pubmed publisher
    ..These as well as LC3B were downregulated in the spleen...
  53. Zhou Y, Ren Y, Cong Y, Mu Y, Yin R, Ding Z. Autophagy induced by bovine viral diarrhea virus infection counteracts apoptosis and innate immune activation. Arch Virol. 2017;162:3103-3118 pubmed publisher
    ..Madin-Darby bovine kidney cells stably expressing microtubule-associated protein 1 light chain 3B (LC3B) with green fluorescent protein (GFP) (GFP-LC3-MDBK cells) and autophagy-deficient MDBKs (shBCN1-MDBK cells) were ..
  54. Espinoza Mellado M, Reyes Picaso C, Garcés Pérez M, Jardón Serrano C, López Villegas E, Giono Cerezo S. Haemophilus influenzae triggers autophagy in HEp-2 cells. Arch Microbiol. 2016;198:199-204 pubmed publisher
    ..We demonstrate that higher expression of the LC3B-II protein was induced, particularly by nontypeable Haemophilus influenzae (NTHi) 49766 and by supernatants, ..
  55. Marzetti E, Calvani R, Lorenzi M, Tanganelli F, Picca A, Bossola M, et al. Association between myocyte quality control signaling and sarcopenia in old hip-fractured patients: Results from the Sarcopenia in HIp FracTure (SHIFT) exploratory study. Exp Gerontol. 2016;80:1-5 pubmed publisher
    ..fusion factor mitofusin (Mfn) 2 and the autophagy mediator microtubule-associated protein 1 light chain 3B (LC3B) was significantly lower in patients with sarcopenia compared with non-sarcopenic controls...
  56. Liu Y, Li S, Zhang S, Cao X, Zhang Y. [Lipopolysaccharide of Porphyromonas gingivalis promotes the autophagy of human gingival fibroblasts]. Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2017;33:315-319 pubmed
    ..Rapamycin was used as a positive control. The expression of LC3B was detected by Western blotting and the distribution of autophagosomes was observed by indirect immunofluorescence ..
  57. Lai K, Killingsworth M, Yong J, Matthews S, Ebrahimi A, McGuinness J, et al. Specific localization of LC3B in autophagosome: A correlative labelling study with nanoparticle in oral squamous cell carcinoma. Exp Mol Pathol. 2017;102:422-427 pubmed publisher
  58. Wei F, Jiang X, Gao H, Gao S. Liquiritin induces apoptosis and autophagy in cisplatin (DDP)-resistant gastric cancer cells in vitro and xenograft nude mice in vivo. Int J Oncol. 2017;51:1383-1394 pubmed publisher
    ..and autophagy both in vitro and in vivo through enhancing cleavage of caspase-8/-9/-3 and PARP, as well as LC3B and Beclin 1 expression...
  59. Wen J, Fang F, Guo S, Zhang Y, Peng X, Sun W, et al. Amyloid ?-Derived Diffusible Ligands (ADDLs) Induce Abnormal Autophagy Associated with A? Aggregation Degree. J Mol Neurosci. 2018;64:162-174 pubmed publisher
    ..In addition, the quantitative Western blot data demonstrated that the ratio of LC3B-II/I was significantly increased, and SQSTM1/p62 decreased over time...
  60. Giatromanolaki A, Sivridis E, Mendrinos S, Koutsopoulos A, Koukourakis M. Autophagy proteins in prostate cancer: relation with anaerobic metabolism and Gleason score. Urol Oncol. 2014;32:39.e11-8 pubmed publisher
    ..the expression of four major autophagy proteins, namely the microtubule-associated protein 1 light chain 3A (LC3A), LC3B, Beclin 1, and p62, together with an enzyme of anaerobic metabolism, the lactate dehydrogenase 5 (LDH5), in ..
  61. Yao X, Lu B, Lü C, Bai Q, Yan D, Wu Y, et al. Solanesol induces the expression of heme oxygenase-1 via p38 and Akt and suppresses the production of proinflammatory cytokines in RAW264.7 cells. Food Funct. 2017;8:132-141 pubmed publisher
    ..Solanesol also elevated the autophagic protein LC3B-II level...
  62. Wu D, Jia C, Chen J, Lin Z, Ruan D, Li X, et al. Autophagic LC3B overexpression correlates with malignant progression and predicts a poor prognosis in hepatocellular carcinoma. Tumour Biol. 2014;35:12225-33 pubmed publisher
    ..this study is aimed to address the clinicopathologic effect of microtubule-associated protein 1 light chain 3B (LC3B) and Beclin-1, as autophagic markers, in HCC patients...
  63. Wurzer B, Zaffagnini G, Fracchiolla D, Turco E, Abert C, Romanov J, et al. Oligomerization of p62 allows for selection of ubiquitinated cargo and isolation membrane during selective autophagy. elife. 2015;4:e08941 pubmed publisher
    ..that selectively link the cargo to the autophagosomal membrane decorated with ATG8 family proteins such as LC3B. Here we show that the human cargo receptor p62/SQSTM-1 employs oligomerization to stabilize its interaction with ..
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    ..Six hours after LPS initiation, activation of the autophagic process, demonstrated by increases in Atg12 and LC3B-II/LC3B-I ratios, and induction of haem oxygenase (HO)-1 and heat-shock protein (HSP) 70 protein expression in ..
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    ..The expression of ULK1 and other autophagic marker (LC3B) were test by Tissue microarray-based immunohistochemistry in 156 operable HCC patients...
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    ..Additionally, we demonstrated that HS induced autophagy, including increased LC3B and Beclin-1 expression and decreased phosphorylation of mTOR and Stat3, as well as phosphorylation of ERK...
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    ..pathway as the main route for aggresomes clearance, CCHE-45 cells displayed increased autophagy flux mediated by MAP1LC3B. MAP1LC3A-Variant1 gene expression was silenced by promoter methylation...
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    ..subunits causes not only juxtanuclear clustering of lysosomes, but also increased levels of the autophagy protein LC3B-II and the receptor SQSTM1...
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    ..In humans, six Atg8 homologs, including MAP1LC3A, MAP1LC3B, MAP1LC3C (refer to LC3A, LC3B, and LC3C hereafter), GABARAP, GABARAPL1, and GABARAPL2 have been reported...
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    ..We have previously shown that FYCO1 binds to LC3B via a 19-amino acid sequence containing a putative core LC3-interacting region (LIR) motif...
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    ..Autophagy has clear implications as a therapeutic target with which to improve the efficacy of neoadjuvant chemoradiotherapy. ..
  73. Kudou M, Shiozaki A, Kosuga T, Shimizu H, Ichikawa D, Konishi H, et al. Heat shock exerts anticancer effects on liver cancer via autophagic degradation of aquaporin 5. Int J Oncol. 2017;50:1857-1867 pubmed publisher
    ..Cycloheximide (CHX) chase experiments and double IF of AQP5 and light chain 3B (LC3B) were performed to investigate the mechanisms underlying changes in AQP5 expression...
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    ..Expression of autophagosome marker LC3B-II and mitochondria-specific autophagy, mitophagy, regulator Park2, were significantly increased in the retinas of ..
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    ..We found that knockdown of PSF markedly decreased the autophagic molecule LC3B in DLD-1 cells but not in HT-29 cells...
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    ..Binding of ALFY to GABARAP is indispensable for its recruitment to LC3B-positive structures and, thus, for the clearance of certain p62 structures by autophagy...
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    ..Western blot revealed that the expression of Beclin-1 and LC3B-II was enhanced, and the phosphorylation levels of the mammalian target of rapamycin (mTOR) protein and p70S6K were ..
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    Microtubule-associated protein 1 light chain 3B (LC3B) is a known marker of autophagy in mammals...
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    ..These data suggest that WDR81 coordinates p62 and LC3C to facilitate autophagic removal of Ub proteins, and provide important insights into CAMRQ2 syndrome, a WDR81-related developmental disorder. ..
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    ..Here, we use purified recombinant proteins to define LC3B interactions with a specific LIR sequence from NEDD4, present a crystal structure showing atomic details of the ..
  81. Chen Z, Lam H, Jin Y, Kim H, Cao J, Lee S, et al. Autophagy protein microtubule-associated protein 1 light chain-3B (LC3B) activates extrinsic apoptosis during cigarette smoke-induced emphysema. Proc Natl Acad Sci U S A. 2010;107:18880-5 pubmed publisher
    ..We identified the autophagic protein microtubule-associated protein 1 light chain-3B (LC3B) as a positive regulator of CS-induced lung epithelial cell death...
  82. Liu K, Zhou D, Ouyang D, Xu L, Wang Y, Wang L, et al. LC3B-II deacetylation by histone deacetylase 6 is involved in serum-starvation-induced autophagic degradation. Biochem Biophys Res Commun. 2013;441:970-5 pubmed publisher
    ..In this study, we found that the acetylation levels of phosphotidylethanolamine (PE)-conjugated LC3B (LC3B-II), which is a critical component of double-membrane autophagosome, were profoundly decreased in HeLa cells ..
  83. Kraft L, Nguyen T, Vogel S, Kenworthy A. Size, stoichiometry, and organization of soluble LC3-associated complexes. Autophagy. 2014;10:861-77 pubmed publisher
    b>MAP1LC3B, an ortholog of yeast Atg8 and a member of the family of proteins formerly also known as ATG8 in mammals (LC3B henceforth in the text), functions in autophagosome formation and autophagy substrate recruitment...
  84. Strappazzon F, Nazio F, Corrado M, Cianfanelli V, Romagnoli A, Fimia G, et al. AMBRA1 is able to induce mitophagy via LC3 binding, regardless of PARKIN and p62/SQSTM1. Cell Death Differ. 2015;22:419-32 pubmed publisher
    ..These results highlight a novel role for AMBRA1 as a powerful mitophagy regulator, through both canonical or noncanonical pathways. ..
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    ..Here, we show that NDP52 also promotes the maturation of autophagosomes via its interaction with LC3A, LC3B, and/or GABARAPL2 through a distinct LC3-interacting region, and with MYOSIN VI...
  86. Riaz Ahmed K, Kanduluru A, Feng L, Fuchs P, Huang P. Antitumor agent 25-epi Ritterostatin GN1N induces endoplasmic reticulum stress and autophagy mediated cell death in melanoma cells. Int J Oncol. 2017;50:1482-1490 pubmed publisher
    ..the ER stress marker CHOP, loss of mitochondrial membrane potential, and lipidation of the autophagy marker protein LC3B. Importantly, normal melanocytes exhibited limited sensitivity to 25-epi Ritterostatin GN1N...
  87. Wnuk A, Rzemieniec J, Lasoń W, Krzeptowski W, Kajta M. Benzophenone-3 Impairs Autophagy, Alters Epigenetic Status, and Disrupts Retinoid X Receptor Signaling in Apoptotic Neuronal Cells. Mol Neurobiol. 2018;55:5059-5074 pubmed publisher determined by the downregulation of autophagy-related genes, decreased autophagosome formation, and reduced LC3B-to-LC3A ratio...
  88. Chiang M, Liang C, Liu C, Pan B, Chen W, Yang Y, et al. Aliskiren Improves Ischemia- and Oxygen Glucose Deprivation-Induced Cardiac Injury through Activation of Autophagy and AMP-Activated Protein Kinase. Front Pharmacol. 2017;8:819 pubmed publisher
    ..Aliskiren increased autophagy, as demonstrated by LC3B-II expression and transmission electron microscopy...
  89. Tumbarello D, Manna P, Allen M, Bycroft M, Arden S, Kendrick Jones J, et al. The Autophagy Receptor TAX1BP1 and the Molecular Motor Myosin VI Are Required for Clearance of Salmonella Typhimurium by Autophagy. PLoS Pathog. 2015;11:e1005174 pubmed publisher
    ..Our study identifies essential new machinery for the autophagy-dependent clearance of Salmonella typhimurium and suggests modulation of myosin VI motor activity as a potential therapeutic target in cellular immunity. ..
  90. Dai L, Bai A, Smith C, Rodriguez P, Yu F, Qin Z. ABC294640, A Novel Sphingosine Kinase 2 Inhibitor, Induces Oncogenic Virus-Infected Cell Autophagic Death and Represses Tumor Growth. Mol Cancer Ther. 2017;16:2724-2734 pubmed publisher
    ..immortalized endothelial cell-line, TIVE-LTC, but not in uninfected TIVE cells, through the upregulation of LC3B protein...
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    ..resulted in regression of tumor cell growth and activation of caspase-independent autophagic cell death, via LC3B-II activation pathway in Hep3B cells...
  92. Adams O, Dislich B, Berezowska S, Schläfli A, Seiler C, Kröll D, et al. Prognostic relevance of autophagy markers LC3B and p62 in esophageal adenocarcinomas. Oncotarget. 2016;7:39241-39255 pubmed publisher
    ..Protein light chain 3 B (LC3B) and p62 are associated with autophagosomal membranes and degraded...