MAN2B1

Summary

Gene Symbol: MAN2B1
Description: mannosidase, alpha, class 2B, member 1
Alias: LAMAN, MANB, lysosomal acid alpha-mannosidase, lysosomal alpha-mannosidase, mannosidase alpha-B, mannosidase, alpha B, lysosomal
Species: human

Top Publications

  1. ncbi Assignment of intrachain disulfide bonds and characterization of potential glycosylation sites of the type 1 recombinant human immunodeficiency virus envelope glycoprotein (gp120) expressed in Chinese hamster ovary cells
    C K Leonard
    Department of Medicinal Chemistry, Genentech, Inc, South San Francisco, California 94080
    J Biol Chem 265:10373-82. 1990
  2. pmc HIV-1 gp120 N-linked glycosylation differs between plasma and leukocyte compartments
    Yung Shwen Ho
    Retroviral Genetics Laboratory, Centre for Virus Research, Westmead Millennium Institute, Westmead Hospital, University of Sydney, Westmead NSW, 2145 Sydney, Australia
    Virol J 5:14. 2008
  3. pmc Env length and N-linked glycosylation following transmission of human immunodeficiency virus Type 1 subtype B viruses
    Yi Liu
    Department of Microbiology, University of Washington School of Medicine, Seattle, Washington 98195 8070, USA
    Virology 374:229-33. 2008
  4. ncbi Folding of the human immunodeficiency virus type 1 envelope glycoprotein in the endoplasmic reticulum
    A Land
    Department of Bio Organic Chemistry, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands
    Biochimie 83:783-90. 2001
  5. ncbi Role of asparagine-linked glycosylation in human immunodeficiency virus type 1 transmembrane envelope function
    D A Dedera
    Department of Medicine, Washington University School of Medicine, St Louis, Missouri 63110
    Virology 187:377-82. 1992
  6. ncbi Glycosylation inhibitors and neuraminidase enhance human immunodeficiency virus type 1 binding and neutralization by mannose-binding lectin
    Melanie L Hart
    Department of Immunology Microbiology, Rush Presbyterian St Luke s Medical Center, 1563 W Congress Parkway, Chicago, IL 60612, USA
    J Gen Virol 84:353-60. 2003
  7. pmc Role of protein N-glycosylation in pathogenesis of human immunodeficiency virus type 1
    D C Montefiori
    Department of Pathology, Vanderbilt University, School of Medicine, Nashville, TN 37232
    Proc Natl Acad Sci U S A 85:9248-52. 1988
  8. pmc Functional role of the glycan cluster of the human immunodeficiency virus type 1 transmembrane glycoprotein (gp41) ectodomain
    E Fenouillet
    Laboratoire de Biologie et Génétique des Pathologies Immunitaires, CNRS URA 1463, France
    J Virol 67:150-60. 1993
  9. ncbi Influence of N-linked glycans in V4-V5 region of human immunodeficiency virus type 1 glycoprotein gp160 on induction of a virus-neutralizing humoral response
    A Bolmstedt
    Department of Clinical Virology, University of Goteborg, Sweden
    J Acquir Immune Defic Syndr Hum Retrovirol 12:213-20. 1996
  10. pmc A single nucleotide exchange in the wzy gene is responsible for the semirough O6 lipopolysaccharide phenotype and serum sensitivity of Escherichia coli strain Nissle 1917
    Lubomir Grozdanov
    Institut für Molekulare Infektionsbiologie, Bayerische Julius Maximilians Universität Würzburg, Rontgenring 11, D 97070 Wurzburg, Germany
    J Bacteriol 184:5912-25. 2002

Scientific Experts

Detail Information

Publications89

  1. ncbi Assignment of intrachain disulfide bonds and characterization of potential glycosylation sites of the type 1 recombinant human immunodeficiency virus envelope glycoprotein (gp120) expressed in Chinese hamster ovary cells
    C K Leonard
    Department of Medicinal Chemistry, Genentech, Inc, South San Francisco, California 94080
    J Biol Chem 265:10373-82. 1990
    ....
  2. pmc HIV-1 gp120 N-linked glycosylation differs between plasma and leukocyte compartments
    Yung Shwen Ho
    Retroviral Genetics Laboratory, Centre for Virus Research, Westmead Millennium Institute, Westmead Hospital, University of Sydney, Westmead NSW, 2145 Sydney, Australia
    Virol J 5:14. 2008
    ..Although it is known that glycosylation changes can dramatically influence virus recognition by the host antibody, the actual contribution of compartmental differences in N-linked glycosylation patterns remains unclear...
  3. pmc Env length and N-linked glycosylation following transmission of human immunodeficiency virus Type 1 subtype B viruses
    Yi Liu
    Department of Microbiology, University of Washington School of Medicine, Seattle, Washington 98195 8070, USA
    Virology 374:229-33. 2008
    ....
  4. ncbi Folding of the human immunodeficiency virus type 1 envelope glycoprotein in the endoplasmic reticulum
    A Land
    Department of Bio Organic Chemistry, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands
    Biochimie 83:783-90. 2001
    ..Here we present an overview of the present knowledge on the folding process of the heavily glycosylated human immunodeficiency virus type 1 (HIV-1) envelope glycoprotein in the ER...
  5. ncbi Role of asparagine-linked glycosylation in human immunodeficiency virus type 1 transmembrane envelope function
    D A Dedera
    Department of Medicine, Washington University School of Medicine, St Louis, Missouri 63110
    Virology 187:377-82. 1992
    ....
  6. ncbi Glycosylation inhibitors and neuraminidase enhance human immunodeficiency virus type 1 binding and neutralization by mannose-binding lectin
    Melanie L Hart
    Department of Immunology Microbiology, Rush Presbyterian St Luke s Medical Center, 1563 W Congress Parkway, Chicago, IL 60612, USA
    J Gen Virol 84:353-60. 2003
    ..This study shows that specific alterations of the N-linked carbohydrates on HIV gp120/gp41 can enhance MBL-mediated neutralization of virus by strengthening the interaction of HIV-1 with MBL...
  7. pmc Role of protein N-glycosylation in pathogenesis of human immunodeficiency virus type 1
    D C Montefiori
    Department of Pathology, Vanderbilt University, School of Medicine, Nashville, TN 37232
    Proc Natl Acad Sci U S A 85:9248-52. 1988
    ..These results demonstrate that protein N-glycosylation is critical to the pathogenesis of HIV-1 at the levels of viral infectivity and cytopathicity but not at the level of virus replication or of host-cell infectibility...
  8. pmc Functional role of the glycan cluster of the human immunodeficiency virus type 1 transmembrane glycoprotein (gp41) ectodomain
    E Fenouillet
    Laboratoire de Biologie et Génétique des Pathologies Immunitaires, CNRS URA 1463, France
    J Virol 67:150-60. 1993
    ....
  9. ncbi Influence of N-linked glycans in V4-V5 region of human immunodeficiency virus type 1 glycoprotein gp160 on induction of a virus-neutralizing humoral response
    A Bolmstedt
    Department of Clinical Virology, University of Goteborg, Sweden
    J Acquir Immune Defic Syndr Hum Retrovirol 12:213-20. 1996
    ..These data indicated that elimination of the three N-linked glycans from gp160 resulted in an altered local antigenic conformation but did not uncover hidden neutralization epitopes, broadening the immune response...
  10. pmc A single nucleotide exchange in the wzy gene is responsible for the semirough O6 lipopolysaccharide phenotype and serum sensitivity of Escherichia coli strain Nissle 1917
    Lubomir Grozdanov
    Institut für Molekulare Infektionsbiologie, Bayerische Julius Maximilians Universität Würzburg, Rontgenring 11, D 97070 Wurzburg, Germany
    J Bacteriol 184:5912-25. 2002
    ..The DNA sequence of the wb(*) gene cluster (11 kb) exhibits no homology to known DNA sequences except manC and manB. Comparison of the genetic structures of the wb(*)(O6) (wb(*) from serotype O6) determinants of strain Nissle 1917 ..
  11. ncbi Deletion of the Escherichia coli O14:K7 O antigen gene cluster
    Slade O Jensen
    School of Molecular and Microbial Sciences, University of Sydney, New South Wales, Australia
    Can J Microbiol 50:299-302. 2004
    ..type strain (Su4411/41) was most likely deleted via homologous recombination between the GDP-mannose pathway genes (manB and manC) of the colanic acid and O antigen gene clusters...
  12. ncbi [Sequence of Escherichia coli O141 O-antigen gene cluster and analyses of its evolutionary history]
    Qing Ke Kong
    College of Life Science, Nankai University, Tianjin 300457, China
    Yi Chuan Xue Bao 31:1448-54. 2004
    ..coli O141. Phylogenetic trees for the rmlB, rmlD, rmlA, and rmlC genes and manB, manC genes were generated and the comparisons were made among different strains...
  13. pmc Cloning and heterologous expression of a beta-D-mannosidase (EC 3.2.1.25)-encoding gene from Thermobifida fusca TM51
    Emese Béki
    Department of Agricultural Biotechnology and Microbiology, Szent Istvan University, Godollo, Hungary
    Appl Environ Microbiol 69:1944-52. 2003
    ..One positive clone was identified, and a beta-mannosidase-encoding gene (manB) was isolated...
  14. pmc Multilocus sequence typing lacks the discriminatory ability of pulsed-field gel electrophoresis for typing Salmonella enterica serovar Typhimurium
    Mohamed K Fakhr
    Department of Veterinary and Microbiological Sciences, North Dakota State University, Fargo, ND 58105, USA
    J Clin Microbiol 43:2215-9. 2005
    ..For the MLST analysis, the manB, pduF, glnA, and spaM genes were amplified by PCR from the same 85 isolates...
  15. pmc DNA sequence-based subtyping and evolutionary analysis of selected Salmonella enterica serotypes
    Sharinne Sukhnanand
    Department of Food Science, 412 Stocking Hall, Cornell University, Ithaca, NY 14853, USA
    J Clin Microbiol 43:3688-98. 2005
    ..typing (MLST) scheme for Salmonella targeting seven housekeeping and virulence genes (panB, fimA, aceK, mdh, icdA, manB, and spaN). A total of eight MLST types were found among the 25 isolates sequenced...
  16. pmc MANBA, CXCR5, SOX8, RPS6KB1 and ZBTB46 are genetic risk loci for multiple sclerosis
    Christina M Lill
    Neuropsychiatric Genetics Group, Department of Vertebrate Genomics, Max Planck Institute for Molecular Genetics, Berlin, Germany
    Brain 136:1778-82. 2013
    ..Our study provides compelling evidence that these five loci are genuine multiple sclerosis susceptibility loci. These results may eventually lead to a better understanding of the underlying disease pathophysiology...
  17. pmc Ceftiofur-resistant Salmonella strains isolated from dairy farms represent multiple widely distributed subtypes that evolved by independent horizontal gene transfer
    S D Alcaine
    Department of Food Science, Cornell University, Ithaca, NY 14853, USA
    Antimicrob Agents Chemother 49:4061-7. 2005
    ..from these seven farms were analyzed for evolutionary relatedness (by DNA sequencing of the Salmonella genes fimA, manB, and mdh), antibiotic resistance profiles, and the presence of bla(CMY-2), a beta-lactamase gene associated with ..
  18. doi Francisella is sensitive to insect antimicrobial peptides
    Malin Vonkavaara
    Department of Clinical Microbiology, Umea University, Umea, Sweden
    J Innate Immun 5:50-9. 2013
    ..e. Attacin, Cecropin, Drosocin and Drosomycin. Furthermore, six bacterial genes, kpsF, manB, lpxF, slt, tolA and pal, were found to be required for resistance to Relish-dependent immune responses, ..
  19. doi Identification of 83 novel alpha-mannosidosis-associated sequence variants: functional analysis of MAN2B1 missense mutations
    Hilde Monica Frostad Riise Stensland
    Division of Child and Adolescent Health, Department of Medical Genetics, University Hospital of North Norway, Tromsø, Norway
    Hum Mutat 33:511-20. 2012
    ..The lysosomal storage disorder alpha-mannosidosis is caused by deficiency of the enzyme lysosomal alpha-mannosidase (MAN2B1)...
  20. pmc Intracellular transport of human lysosomal alpha-mannosidase and alpha-mannosidosis-related mutants
    Gaute Hansen
    Department of Medical Biochemistry, University of Tromsø, N 9037 Tromsø, Norway
    Biochem J 381:537-46. 2004
    Human LAMAN (lysosomal a-mannosidase) was synthesized as a 120 kDa precursor in transfected COS cells [African-green-monkey kidney cells], which was partly secreted as a single-chain form and partly sorted to the lysosomes being ..
  21. ncbi Efficacy of enzyme replacement therapy in alpha-mannosidosis mice: a preclinical animal study
    Diego Prieto Roces
    Georg August Univeresität Göttingen, Abt Biochemie II, Heinrich Düker Weg 12, 37073 Gottingen, Germany
    Hum Mol Genet 13:1979-88. 2004
    ..in a mouse model of alpha-mannosidosis after intravenous administration of lysosomal acid alpha-mannosidase (LAMAN) from bovine kidney, and human and mouse recombinant LAMAN...
  22. pmc Glycosylation site-specific analysis of HIV envelope proteins (JR-FL and CON-S) reveals major differences in glycosylation site occupancy, glycoform profiles, and antigenic epitopes' accessibility
    Eden P Go
    Department of Chemistry, University of Kansas, Lawrence, Kansas 66045, USA
    J Proteome Res 7:1660-74. 2008
    ....
  23. pmc Multilocus sequence typing supports the hypothesis that cow- and human-associated Salmonella isolates represent distinct and overlapping populations
    S D Alcaine
    Department of Food Science, 412 Stocking Hall, Cornell University, Ithaca, NY 14853, USA
    Appl Environ Microbiol 72:7575-85. 2006
    ..using serotyping and a multilocus sequence typing (MLST) scheme based on the sequencing of three genes (fimA, manB, and mdh). The 335 isolates were differentiated into 52 serotypes and 72 sequence types (STs)...
  24. pmc Multilocus sequence typing for characterization of clinical and environmental salmonella strains
    Mamuka Kotetishvili
    Division of Molecular Epidemiology, Department of Epidemiology and Preventive Medicine, University of Maryland School of Medicine, 10 South Pine Street, Baltimore, MD 21201, USA
    J Clin Microbiol 40:1626-35. 2002
    Multilocus sequence typing (MLST) based on the 16S RNA, pduF, glnA, and manB genes was developed for Salmonella, and its discriminatory ability was compared to those of pulsed-field gel electrophoresis (PFGE) and serotyping...
  25. pmc Comparative phosphoproteomics reveals components of host cell invasion and post-transcriptional regulation during Francisella infection
    Ernesto S Nakayasu
    Biological Science Division, Pacific Northwest National Laboratory, Richland, Washington
    Mol Cell Proteomics 12:3297-309. 2013
    ..Four of the identified hyper-cytotoxic strains (lpcC, manB, manC, and kdtA) had an impaired lipopolysaccharide (LPS) synthesis and produced an exposed lipid A lacking the O-..
  26. doi CD44 variant isoforms control experimental autoimmune encephalomyelitis by affecting the lifespan of the pathogenic T cells
    Lizette Ghazi-Visser
    Department of Immunology, University Medical Center Rotterdam, Rotterdam, The Netherlands
    FASEB J 27:3683-701. 2013
    ..Targeting these short CD44(v) isoform regions may reduce inflammatory processes and clinical symptoms in MS...
  27. pmc Traffic of human α-mannosidase in plant cells suggests the presence of a new endoplasmic reticulum-to-vacuole pathway without involving the Golgi complex
    Francesca De Marchis
    Istituto di Genetica Vegetale, Consiglio Nazionale delle Ricerche, 06128 Perugia, Italy
    Plant Physiol 161:1769-82. 2013
    ..This work is focused on the transport in transgenic tobacco (Nicotiana tabacum) plants of a human α-mannosidase, MAN2B1, which is a lysosomal enzyme involved in the turnover of N-linked glycoproteins and can be used in enzyme ..
  28. pmc O antigen is the receptor of Vibrio cholerae serogroup O1 El Tor typing phage VP4
    Jialiang Xu
    National Institute for Communicable Disease Control and Prevention and State Key Laboratory for Infectious Disease Prevention and Control, Chinese Center for Disease Control and Prevention, Changping, Beijing, China
    J Bacteriol 195:798-806. 2013
    ..Mutations in the manB, wbeE, and wbeU genes caused failure of adsorption of VP4 to these strains, whereas the observed amino acid residue ..
  29. ncbi Increased sensitivity to chloramphenicol by inactivation of manB in Streptomyces coelicolor
    Thangamani Rajesh
    Department of Microbial Engineering, College of Engineering, Konkuk University, Seoul, 143 701, Korea
    J Microbiol Biotechnol 22:1324-9. 2012
    Phosphomannomutase (ManB) is involved in the biosynthesis of GDP-mannose, which is vital for numerous processes such as synthesis of carbohydrates, production of alginates and ascorbic acid, and post-translational modification of proteins...
  30. doi Loss of phosphomannomutase activity enhances actinorhodin production in Streptomyces coelicolor
    Yung Hun Yang
    School of Chemical and Biological Engineering, Seoul National University, Seoul, Korea
    Appl Microbiol Biotechnol 86:1485-92. 2010
    Phosphomannomutase (ManB), whose main function is the conversion of mannose-6-phosphate to mannose-1-phosphate, is involved in biosynthesis of GDP-mannose for numerous processes such as synthesis of structural carbohydrates, production of ..
  31. pmc Comparative transcriptional study of the putative mannose donor biosynthesis genes in virulent Mycobacterium tuberculosis and attenuated Mycobacterium bovis BCG strains
    Tracy L Keiser
    Center for Microbial Interface Biology, The Ohio State University, 460 W 12th Avenue, 1004 Biomedical Research Tower, Columbus, OH 43210, USA
    Infect Immun 79:4668-73. 2011
    ..Results for M. tuberculosis extracted from within macrophages show that whiB2 is highly expressed and manB and manC are moderately expressed during infection...
  32. pmc A multi-species comparative structural bioinformatics analysis of inherited mutations in alpha-D-mannosidase reveals strong genotype-phenotype correlation
    Javed Mohammed Khan
    Department of Chemistry and Biomolecular Sciences and ARC center of excellence in Bioinformatics, Macquarie University, NSW 2109, Australia
    BMC Genomics 10:S33. 2009
    ..Mutations in the gene (MAN2B1) encoding lysosomal alpha-D-mannosidase cause improper coding, resulting in dysfunctional or non-functional ..
  33. doi Modulation of guanosine 5'-diphosphate-D-mannose metabolism in recombinant Escherichia coli for production of guanosine 5'-diphosphate-L-fucose
    Won Heong Lee
    Department of Agricultural Biotechnology, Seoul National University, Seoul 151 921, Republic of Korea
    Bioresour Technol 100:6143-8. 2009
    ..coli BL21star(DE3) strain overexpressing manB, manC, gmd and wcaG genes...
  34. pmc DNA polymorphism analysis of Brucella lipopolysaccharide genes reveals marked differences in O-polysaccharide biosynthetic genes between smooth and rough Brucella species and novel species-specific markers
    Michel S Zygmunt
    INRA, UR1282, Infectiologie Animale et Sante Publique, IASP, Nouzilly, France
    BMC Microbiol 9:92. 2009
    ..In smooth brucellae, lipopolysaccharide is made of lipid A-core oligosaccharide and N-formylperosamine O-polysaccharide. B. ovis and B. canis (rough species) lack the O-polysaccharide...
  35. ncbi Cloning, sequence, expression and characterization of human beta-mannosidase
    Zahoor Qadir Samra
    Institute of Biochemistry and Biotechnology, Quaid i Azam Campus, University of the Punjab, Lahore, Pakistan
    Acta Biochim Pol 55:479-90. 2008
    Beta-mannosidase (EC 3.2.1.25, MANB) dissects the non-reducing end of N-linked mannose moieties of glycoproteins in eukaryotic cells. The human beta-mannosidase gene was amplified by RT-PCR, cloned and sequenced...
  36. pmc Alpha-mannosidosis
    Dag Malm
    Department of Gastroenterology, University Hospital of North Norway, NO 9038, Norway
    Orphanet J Rare Dis 3:21. 2008
    ..Alpha-mannosidosis is inherited in an autosomal recessive fashion and is caused by mutations in the MAN2B1 gene located on chromosome 19 (19 p13.2-q12)...
  37. doi Characterisation of Yersinia pestis isolates from natural foci of plague in the Republic of Georgia, and their relationship to Y. pestis isolates from other countries
    T Revazishvili
    Emerging Pathogens Institute and Department of Molecular Genetics and Microbiology, University of Florida College of Medicine, Gainesville, FL 32610, USA
    Clin Microbiol Infect 14:429-36. 2008
    ..using multilocus sequence typing (MLST), based on sequence data for the 16S rRNA, hsp60, glnA, gyrB, recA, manB, thrA and tmk loci. Four virulence gene loci (caf1, lcrV, psaA and pla) were also sequenced and analysed...
  38. doi Human α-mannosidase produced in transgenic tobacco plants is processed in human α-mannosidosis cell lines
    Francesca De Marchis
    Institute of Plant Genetics, Italian National Council of Research CNR, Perugia, Italy
    Plant Biotechnol J 9:1061-73. 2011
    Deficiency in human lysosomal α-mannosidase (MAN2B1) results in α-mannosidosis, a lysosomal storage disorder; patients present a wide range of neurological, immunological, and skeletal symptoms caused by a multisystemic accumulation of ..
  39. doi Human lysosomal α-D-mannosidase regulation in promyelocytic leukaemia cells
    Lorena Urbanelli
    Department of Experimental Medicine and Biochemical Sciences, University of Perugia, Via del Giochetto, 06126 Perugia, Italy
    Biosci Rep 31:477-87. 2011
    ..The MAN2B1 transcript level correlates with enzyme activity, indicating a transcriptional up-regulation of the α-D-..
  40. doi Molecular and cellular characterization of novel {alpha}-mannosidosis mutations
    Elina Kuokkanen
    Department of Biochemistry and Food Chemistry, University of Turku, Turku, Finland
    Hum Mol Genet 20:2651-61. 2011
    Mannosidosis is a lysosomal storage disorder caused by mutations in the MAN2B1 gene. The clinical presentation of α-mannosidosis is variable, but typically includes mental retardation, skeletal abnormalities and immune deficiency...
  41. pmc The phosphoproteome of the minimal bacterium Mycoplasma pneumoniae: analysis of the complete known Ser/Thr kinome suggests the existence of novel kinases
    Sebastian R Schmidl
    Department of General Microbiology, Georg August Universitat Gottingen, Grisebachstrasse 8, D 37077 Gottingen, Germany
    Mol Cell Proteomics 9:1228-42. 2010
    ..Only one single protein with an identified phosphorylation site, a phosphosugar mutase (ManB in M...
  42. pmc Impaired lysosomal trimming of N-linked oligosaccharides leads to hyperglycosylation of native lysosomal proteins in mice with alpha-mannosidosis
    Markus Damme
    Abt Biochemie II, Georg August Universitat Gottingen, Humboldtallee 23, 37073 Gottingen, Germany
    Mol Cell Biol 30:273-83. 2010
    Alpha-mannosidosis is caused by the genetic defect of the lysosomal alpha-d-mannosidase (LAMAN), which is involved in the breakdown of free alpha-linked mannose-containing oligosaccharides originating from glycoproteins with N-linked ..
  43. pmc Mutations of Francisella novicida that alter the mechanism of its phagocytosis by murine macrophages
    Xin He Lai
    Department of Molecular Microbiology and Immunology, Oregon Health and Science University, Portland, Oregon, United States of America
    PLoS ONE 5:e11857. 2010
    ..Surprisingly, we found that F. novicida with mutations in four genes (lpcC, manB, manC and kdtA) were taken up by and killed macrophages at a much higher rate than the parent strain, even upon ..
  44. doi Synthesis of GDP-mannose using coupling fermentation of recombinant Escherichia coli
    Jia Honghong
    Key Laboratory of Industrial Microbiology, Ministry of Education, College of Biotechnology, Tianjin University of Science and Technology, Tianjin, 300457, People s Republic of China
    Biotechnol Lett 33:1145-50. 2011
    Glucokinase (glk), phosphomannomutase (manB), and mannose-1-phosphate guanylytransferase (manC) are needed for the biosynthesis of GDP-mannose. A recombinant E...
  45. doi A celluloytic complex from Clostridium cellulovorans consisting of mannanase B and endoglucanase E has synergistic effects on galactomannan degradation
    Sang Duck Jeon
    School of Life Sciences and Biotechnology, Korea University, Seoul 136 701, Republic of Korea
    Appl Microbiol Biotechnol 90:565-72. 2011
    ..In this study, a mannanase gene, manB from C. cellulovorans, was expressed in Escherichia coli. The optimal pH of a purified enzyme was around pH 7...
  46. pmc Crystallization and preliminary crystallographic analysis of β-mannanase from Bacillus licheniformis
    Chomphunuch Songsiriritthigul
    Synchrotron Light Research Institute, Nakhon Ratchasima, Thailand
    Acta Crystallogr Sect F Struct Biol Cryst Commun 67:217-20. 2011
    The mannan endo-1,4-β-mannosidase (ManB) from Bacillus licheniformis strain DSM13 was overexpressed in Escherichia coli...
  47. doi Genetic diversity of capsular polysaccharide biosynthesis in Klebsiella pneumoniae clinical isolates
    Hung Yu Shu
    Department of Bioscience Technology, Chang Jung Christian University, Tainan County, Taiwan, ROC
    Microbiology 155:4170-83. 2009
    ..The rmlBADC genes were found in CPS K9-positive, K14-positive and K52-positive strains, while manC and manB were found in K1, K2, K5, K14, K62 and two undefined strains...
  48. pmc Human lysosomal alpha-mannosidases exhibit different inhibition and metal binding properties
    Meenakshi Venkatesan
    Ontario Cancer Institute, Division of Cancer Genomics and Proteomics, Toronto, Ontario M5G 1L7, Canada
    Protein Sci 18:2242-51. 2009
    ..the lysosomal mannosidase family, the broad substrate specificity enzyme human lysosomal alpha-mannosidase (hLM, MAN2B1) and the human core alpha-1, 6-specific mannosidase (hEpman, MAN2B2) act in a complementary fashion on different ..
  49. doi Co-transcription of the celC gene cluster in Clostridium thermocellum
    Michael Newcomb
    Department of Chemical Engineering, University of Rochester, Room 206 Gavett Hall, Rochester, NY 14627 0166, USA
    Appl Microbiol Biotechnol 90:625-34. 2011
    ..We mapped the transcripts of the six-gene cluster celC-glyR3-licA-orf4-manB-celT and determined their transcription initiation sites by primer extension...
  50. doi Glycoside hydrolase inventory drives plant polysaccharide deconstruction by the extremely thermophilic bacterium Caldicellulosiruptor saccharolyticus
    Amy L VanFossen
    Department of Chemical and Biomolecular Engineering, North Carolina State University, Raleigh, NC 27695 7905, USA
    Biotechnol Bioeng 108:1559-69. 2011
    ..CelA-ManB (Csac_1076-Csac_1080) encodes four multi-domain, extracellular GHs, including CelB (Csac_1078), a 118 kDa ..
  51. pmc Phosphoproteomics of Klebsiella pneumoniae NTUH-K2044 reveals a tight link between tyrosine phosphorylation and virulence
    Miao Hsia Lin
    Institute of Biochemical Sciences, College of Life Sciences, National Taiwan University, Taipei 106, Taiwan
    Mol Cell Proteomics 8:2613-23. 2009
    ..three of the identified tyrosine phosphorylated proteins, namely protein-tyrosine kinase (Wzc), phosphomannomutase (ManB), and undecaprenyl-phosphate glycosyltransferase (WcaJ), were found to be distributed in the cps locus and thus ..
  52. pmc Identification of type 3 fimbriae in uropathogenic Escherichia coli reveals a role in biofilm formation
    Cheryl Lynn Y Ong
    School of Molecular and Microbial Sciences, Centre for Microscopy and Microanalysis, University of Queensland, Brisbane, Australia
    J Bacteriol 190:1054-63. 2008
    ..Mutants containing insertions in genes involved in O antigen synthesis (rmlC and manB) and capsule synthesis (kpsM) possessed enhanced biofilm phenotypes...
  53. ncbi Early onset alpha-mannosidosis with slow progression in three Hispanic males
    Michael J Lyons
    Greenwood Genetic Center, Greenwood, South Carolina, USA
    Dev Med Child Neurol 49:854-7. 2007
    ..They were aged between 6 and 24 years at their last assessment. Homozygous mutations in the MAN2B1 gene were found in all three patients, one of which is a newly reported mutation...
  54. ncbi Synteny mapping of four genes from the short arm of human chromosome 19 to bovine chromosome 7
    Z X Gu
    Department of Animal Sciences, University of Wisconsin Madison, 53706, USA
    Cytogenet Cell Genet 79:225-7. 1997
    ..oligomeric matrix protein (COMP), lymphoblastic leukemia derived sequence 1 (LYL1), lysosomal alpha-mannosidase (MANB), and RAS oncogene family member RAB3A...
  55. ncbi Characterization of the human MANB gene encoding lysosomal alpha-D-mannosidase
    N Wakamatsu
    First Department of Internal Medicine, School of Medicine, The University of Tokushima, Japan
    Gene 198:351-7. 1997
    Genomic clones of human MANB gene encoding the lysosomal enzyme, alpha-mannosidase, have been isolated, sequenced and analyzed. The human MANB gene spans approximately 22 kb and consists of 24 exons...
  56. ncbi Cloning, expression, purification, and characterization of the murine lysosomal acid alpha-mannosidase
    R K Merkle
    Complex Carbohydrate Research Center and the Department of Biochemistry and Molecular Biology, University of Georgia, Athens 30602, USA
    Biochim Biophys Acta 1336:132-46. 1997
    ..5 kb RNA transcript in all murine tissues tested. A minor transcript of 5.4 kb was also detected in some murine tissues similar to the alternatively spliced transcripts that have been previously identified in human tissues...
  57. ncbi Genomic structure of the human lysosomal alpha-mannosidase gene (MANB)
    H M Riise
    Department of Medical Biochemistry, Institute of Medical Biology, University of Tromsø, Norway
    Genomics 42:200-7. 1997
    Lysosomal alpha-mannosidase (LAMAN) (EC 3.2.1.24) is an exoglycosidase involved in the ordered degradation of N-linked oligosaccharides. Lack of LAMAN activity leads to the lysosomal storage disorder alpha-mannosidosis (MIM No. 248500)...
  58. ncbi alpha-Mannosidosis: functional cloning of the lysosomal alpha-mannosidase cDNA and identification of a mutation in two affected siblings
    O Nilssen
    Department of Medical Genetics, University Hospital and University of Tromso, Norway
    Hum Mol Genet 6:717-26. 1997
    ..autosomal recessive lysosomal storage disorder resulting from deficient activity of lysosomal alpha-mannosidase (LAMAN) (EC 3.2.1.24)...
  59. pmc Polymorphism, duplication, and IS1-mediated rearrangement in the chromosomal his-rfb-gnd region of Escherichia coli strains with group IA and capsular K antigens
    J Drummelsmith
    Department of Microbiology, University of Guelph, Ontario, Canada
    J Bacteriol 179:3232-8. 1997
    ..Previously, we reported a duplication of the manC-manB genes, encoding enzymes involved in GDP-mannose formation, upstream of rfb in strain E69 (P. Jayaratne et al., J...
  60. ncbi Effect of various glycosidase treatments on the resistance of the HIV-1 envelope to degradation
    M J Papandreou
    CNRS, UMR 6560, IFR Jean Roche, Faculte de Medecine Nord, Marseille, France
    FEBS Lett 406:191-5. 1997
    ..Thus, partial deglycosylation increases Env sensitivity to proteases but only its total deglycosylation alters its stability...
  61. ncbi Cloning, expression, purification, and characterization of the human broad specificity lysosomal acid alpha-mannosidase
    Y F Liao
    Complex Carbohydrate Research Center and the Department of Biochemistry and Molecular Biology, University of Georgia, Athens, Georgia 30602, USA
    J Biol Chem 271:28348-58. 1996
    ..Human chromosome mapping of the mannosidase gene confirmed that the functional gene maps to the MANB locus on chromosome 19...
  62. pmc Infectivities of human and other primate lentiviruses are activated by desialylation of the virion surface
    H Hu
    Department of Viral Infection, Institute of Medical Science, University of Tokyo, Minato ku, Japan
    J Virol 70:7462-70. 1996
    ..Thus, the sialylation catalyzed by host cell pathways appeared to reduce the infectivity of human and nonhuman primate lentiviruses. Our results further suggested that desialylation would help increase the titers of HIV-based vectors...
  63. ncbi Localization and genomic structure of human deoxyhypusine synthase gene on chromosome 19p13.2-distal 19p13.1
    E Mantuano
    Istituto di Medicina Sperimentale, CNR, Rome, Italy
    Gene 215:153-7. 1998
    ..2-distal 19p13.1 between MANB and JUNB...
  64. ncbi Human lysosomal alpha-mannosidase: isolation and nucleotide sequence of the full-length cDNA
    V L Nebes
    Dept of Ophthalmology, Allegheny Singer Research Institute, Pittsburgh, PA 15212
    Biochem Biophys Res Commun 200:239-45. 1994
    ..The cDNA maps proximal to the centromere on chromosome 19q, the same locus as the MANB gene...
  65. pmc Partial sequence of the purified protein confirms the identity of cDNA coding for human lysosomal alpha-mannosidase B
    C Emiliani
    Dipartimento di Biologia Cellulare e Molecolare, Universita degli Studi di Perugia, Italy
    Biochem J 305:363-6. 1995
    ..Res. Commun. 200, 239-245] and to locate the position of this polypeptide within the total deduced amino acid sequence. This finding will therefore provide a firm foundation for the characterization of alpha-mannosidosis mutations...
  66. ncbi Glycosylation inhibitors block the expression of LAV/HTLV-III (HIV) glycoproteins
    H A Blough
    Biochem Biophys Res Commun 141:33-8. 1986
    ..The reverse transcriptase was not affected by the compounds. Glycosylation inhibitors may be considered for the chemotherapy of AIDS or AIDS-related complex, or chemoprophylaxis of HIV-positive individuals...
  67. ncbi Regional assignment of five genes on human chromosome 19
    Y Kaneda
    Chromosoma 95:8-12. 1987
    ..chromosome 19 (PVS, E11S and RDRC) were lost in HM76Dd40-6, while six other genes (C3, LDLR, EF2, GPI, PEPD and MANB) were retained...
  68. pmc Electrophoretic abnormalities of lysosomal enzymes in mucolipidosis fibroblast lines
    M J Champion
    Am J Hum Genet 29:149-63. 1977
    ..These results are consistent with the mucolipidosis defect(s) being associated with abnormal post-translatinal processing of multiple lysosomal enzymes and adenosine deaminase-d...
  69. pmc Characterization of human liver alpha-D-mannosidase purified by affinity chromatography
    N C Phillips
    Biochem J 153:579-87. 1976
    ..These results validate the use of synthetic substrates for determining the mannosidosis genotype. They are also further evidence that mannosidosis is a lysosomal storage disease resulting from a deficiency of acidic alpha-mannosidase...
  70. ncbi Overexpression of Mycobacterium tuberculosis manB, a phosphomannomutase that increases phosphatidylinositol mannoside biosynthesis in Mycobacterium smegmatis and mycobacterial association with human macrophages
    Travis R McCarthy
    Department of Medicine and Molecular Virology, The Center for Microbial Interface Biology, and the Division of Infectious Diseases, The Ohio State University, Columbus, OH 43210, USA
    Mol Microbiol 58:774-90. 2005
    ..We conclude that MtmanB encodes a functional PMM involved in the biosynthesis of mannosylated lipoglycans that participate in the association of mycobacteria with macrophage phagocytic receptors...
  71. ncbi Funtional characterization of four novel MAN2B1 mutations causing juvenile onset alpha-mannosidosis
    M G Pittis
    Unità Malattie Metaboliche, IRCCS Burlo Garofolo, Trieste, Italy
    Clin Chim Acta 375:136-9. 2007
    ..Finally, the c.2660delC causes a frameshift introducing a premature stop codon (p.T887SfsX45), presuming to be a severe mutation...
  72. ncbi [Occurrence of selected genes of the Klebsiella pneumoniae clusters waa and wb for lipolysaccharide synthesis in reference and epidemic strains]
    Rafał Gierczyński
    Zakład Bakteriologii PZH w Warszawie
    Med Dosw Mikrobiol 57:383-93. 2005
    ..and 6 casual isolates for the existence of 7 (waaA, waaE, waaL, waaQ, waaZ, waaX and uge) and 4 (wbdA, wbdC, manB, wbbO) genes of the waa and wb clusters for LPS biosynthesis...
  73. ncbi Multivariate neurocognitive and emotional profile of a mannosidosis murine model for therapy assessment
    Karen Caeyenberghs
    Laboratory of Biological Psychology, Department of Psychology, KU Leuven, Belgium
    Neurobiol Dis 23:422-32. 2006
    alpha-Mannosidosis is a lysosomal storage disorder caused by lysosomal alpha-mannosidase (LAMAN) deficiency that leads to neurocognitive dysfunctions, psychotic symptoms and emotional changes in human patients...
  74. ncbi Development of an efficient production method for beta-mannosidase by the creation of an overexpression system in Aspergillus aculeatus
    S Kanamasa
    Department of Life Science, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Kanagawa, Japan
    Lett Appl Microbiol 45:142-7. 2007
    To develop an overexpression system in Aspergillus aculeatus in order to establish an efficient overproduction method of beta-mannosidase (MANB).
  75. pmc Limited genetic diversity in Salmonella enterica serovar Enteritidis PT13
    Adam B Olson
    National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, MB, Canada
    BMC Microbiol 7:87. 2007
    ..Enteritidis PT13...
  76. ncbi Sisters with alpha-mannosidosis and systemic lupus erythematosus
    Maki Urushihara
    Department of Paediatrics, School of Medicine, University of Tokushima, Kuramoto cho 3 chome, 770 8503 Tokushima, Japan
    Eur J Pediatr 163:192-5. 2004
    Alpha-mannosidosis is an autosomal recessive disorder caused by deficiency of lysosomal alpha-mannosidase (LAMAN). Here, we report two sisters with alpha-mannosidosis who developed systemic lupus erythematosus (SLE)...
  77. doi Low-dose photons modify liver response to simulated solar particle event protons
    Daila S Gridley
    Department of Radiation Medicine, Molecular Radiation Biology Laboratories, Loma Linda University and Medical Center, Loma Linda, California 92354, USA
    Radiat Res 169:280-7. 2008
    ..those up-regulated after either photons or the simulated SPE alone (photons, Cstb; simulated SPE, Dctn2, Khsrp, Man2b1, Snrp70; photons + simulated SPE, Casp1, Col1a1, Hspcb, Il6st, Rpl28, Spnb2)...
  78. ncbi Molecular and biochemical characterization of endo-beta-mannanases from germinating coffee (Coffea arabica) grains
    P Marraccini
    Department of Plant Science, Nestle Research Centre, Tours, France
    Planta 213:296-308. 2001
    ..In the present paper, we report the cloning and sequencing of two endo-beta-mannanase cDNAs (manA and manB) by different strategies from Coffea arabica L....
  79. pmc Molecular characterization of Lactobacillus plantarum genes for beta-ketoacyl-acyl carrier protein synthase III (fabH) and acetyl coenzyme A carboxylase (accBCDA), which are essential for fatty acid biosynthesis
    P Kiatpapan
    Department of Biotechnology, Graduate School of Engineering, Yamada oka, Suita, Osaka 565 0871, Japan
    Appl Environ Microbiol 67:426-33. 2001
    ..We identified six potential open reading frames, namely, manB, fabH, accB, accC, accD, and accA, in that order...
  80. ncbi Characterization of a novel alpha-mannosidosis-causing mutation and its use in leukocyte genotyping after bone marrow transplantation
    H M Frostad Riise
    Department of Medical Biochemistry, University of Tromsø, Norway
    Hum Genet 104:106-7. 1999
    Alpha-Mannosidosis is a lysosomal storage disorder caused by deficiency of lysosomal alpha-mannosidase (LAMAN). Major symptoms include mental retardation, skeletal changes and recurrent infections...
  81. pmc Spectrum of mutations in alpha-mannosidosis
    T Berg
    Department of Medical Genetics, University Hospital and University of Tromso, N 9037, Norway
    Am J Hum Genet 64:77-88. 1999
    alpha-Mannosidosis is an autosomal recessive disorder caused by deficiency of lysosomal alpha-mannosidase (LAMAN)...
  82. pmc Missense and nonsense mutations in the lysosomal alpha-mannosidase gene (MANB) in severe and mild forms of alpha-mannosidosis
    Y Gotoda
    First Department of Internal Medicine, School of Medicine, The University of Tokushima, Japan
    Am J Hum Genet 63:1015-24. 1998
    ..analysis or direct sequencing, to analyze the 24 exons and intron/exon boundaries of the alpha-mannosidase gene (MANB) from five patients...
  83. ncbi [Sequence of Escherichia coli O11 O-antigen gene cluster and identification of molecular markers specific to O11]
    Wei Wang
    TEDA School of Biological Sciences, Nankai University, Tianjin 300457, China
    Wei Sheng Wu Xue Bao 46:341-6. 2006
    ..glucosamine-4-epimerase gene (gne), genes responsible for the biosynthesis of GDP-L-fucose (gmd, fcl, gmm, manC, manB), glycosyl transferase genes, O-unit flippase gene (wzx) and O-antigen polymerase gene (wzy)...
  84. pmc Efficient recombinant expression and secretion of a thermostable GH26 mannan endo-1,4-beta-mannosidase from Bacillus licheniformis in Escherichia coli
    Chomphunuch Songsiriritthigul
    School of Biotechnology, Institute of Agricultural Technology, Suranaree University of Technology, 111 University Avenue, Nakhon Ratchasima, Thailand
    Microb Cell Fact 9:20. 2010
    ..and has been used extensively for large-scale industrial production of various enzymes; however, there has been no previous report on the cloning and expression of mannan endo-1,4-beta-mannosidase gene (manB) from B. licheniformis.
  85. ncbi Alpha-mannosidosis and mutational analysis in a Turkish patient
    Akgun Olmez
    Section of Pediatric Nutrition and Metabolism, Hacettepe University Faculty of Medicine, Ankara, Turkey
    Turk J Pediatr 45:46-50. 2003
    ..Enzyme studies on white cells confirmed a deficiency of alpha-mannosidase activity, which was 2.6 micromol/g/hr. Her DNA analysis showed a S453Y mutation...
  86. ncbi Identification and characterization of five novel MAN2B1 mutations in Italian patients with alpha-mannosidosis
    Michele Sbaragli
    Dipartimento di Medicina Interna, Universita degli Studi di Perugia, Perugia, Italy
    Hum Mutat 25:320. 2005
    ..on six Italian families with alpha-mannosidosis type II allowed the identification of five new mutations in the MAN2B1 gene: c.157G>T, c.562C>T, c.599A>T, c.293dupA, c.2402G>A (p.E53X, p.R188X, p.H200L, p.Y99VfsX61, p...
  87. ncbi Effect of N-(3-phenyl-2-propenyl)-1-deoxynojirimycin on the lectin binding to HIV-1 glycoproteins
    H Shimizu
    AIDS Research Center, National Institute of Health, Tokyo, Japan
    Jpn J Med Sci Biol 43:75-87. 1990
    ..ppDNM potentially inhibited HIV-1-induced syncytium formation and viral infectivity of HIV-1 without cytotoxicity. The lectin binding assay showed that ppDNM treatment reduced Con A binding to gp120 of HIV-1...
  88. ncbi Site-specific N-glycosylation and oligosaccharide structures of recombinant HIV-1 gp120 derived from a baculovirus expression system
    J C Yeh
    Department of Biochemistry and Molecular Biology, University of Oklahoma Health Sciences Center, Oklahoma City 73190
    Biochemistry 32:11087-99. 1993
    ..These results demonstrate that N-glycosylation of rgp120 in the baculovirus expression system occurs at all potential sites and is site specific in terms of oligosaccharide structures...

Research Grants30

  1. Gene transfer & NMR studies in alpha-mannosidosis brain
    John Wolfe; Fiscal Year: 2006
    ..In this project we will study an inherited deficiency of acidic alpha-D-mannosidase (MANB), which results in the accumulation of undegraded mannose-rich oligosaccharides in cellular lysosomes, prominently ..
  2. Gene Transfer and NMR Studies in Alpha-Mannosidosis Brain
    John H Wolfe; Fiscal Year: 2010
    ..will use a cat model of human alpha- mannosidosis (AMD), caused by a mutation in the lysosomal alpha-mannosidase (LAMAN) gene...
  3. Gene Transfer and NMR Studies in Alpha-Mannosidosis Brain
    John H Wolfe; Fiscal Year: 2010
    ..will use a cat model of human alpha- mannosidosis (AMD), caused by a mutation in the lysosomal alpha-mannosidase (LAMAN) gene...
  4. Altered M. tuberculosis Mannosylation and the Macrophage
    Larry S Schlesinger; Fiscal Year: 2010
    ..One such gene is M.tb manB which we have found encodes a functional phosphomannomutase. Overexpression of manB in M...
  5. IN UTERO TRANSPLANTATION FOR LYSOSOMAL STORAGE DISEASE
    JANIS ABKOWITZ; Fiscal Year: 2002
    ..cells and in utero gene transfer (with FeLV-pseudotyped, retroviral vectors containing marker genes and/or MANB cDNA)...
  6. TB and Innate Immune Regulation of Lung Macrophages
    Larry Schlesinger; Fiscal Year: 2009
    ..abstract_text> ..
  7. Interdisciplinary Study of the Microbe-Host Interface
    Larry Schlesinger; Fiscal Year: 2007
    ....
  8. Mycobacterium tuberculosis lipoarabinomannan metabolism within human macrophages
    Larry Schlesinger; Fiscal Year: 2007
    ..Characterization of intracellular ManLAM metabolism using biochemical means would represent a significant advance for the field in terms of identifying new molecular targets for diagnostics, therapies and vaccines. ..
  9. Altered Mycobacterium tuberculosis Mannosylation and the Macrophage
    Larry Schlesinger; Fiscal Year: 2007
    ..The assembled investigators will combine techniques in genetics, biochemistry, and cell biology to accomplish the goals of this proposal. ..
  10. MACROPHAGE COMPLEMENT RECEPTORS IN TUBERCULOSIS
    Larry Schlesinger; Fiscal Year: 2002
    ..tb-MP interaction in order to enhance our understanding of disease pathogenesis. Ultimately these studies may help to define specific novel therapeutic targets. ..
  11. TB and Innate Immune Regulation of Lung Macrophages
    Larry Schlesinger; Fiscal Year: 2007
    ..abstract_text> ..