lysyl hydroxylase

Summary

Gene Symbol: lysyl hydroxylase
Description: procollagen-lysine, 2-oxoglutarate 5-dioxygenase 1
Alias: EDS6, LH1, LLH, PLOD, lysine hydroxylase, lysyl hydroxlase 1, procollagen-lysine 1, 2-oxoglutarate 5-dioxygenase 1
Species: human

Top Publications

  1. ncbi Identification of arginine-700 as the residue that binds the C-5 carboxyl group of 2-oxoglutarate in human lysyl hydroxylase 1
    K Passoja
    Biocenter and Department of Medical Biochemistry, University of Oulu, Finland
    FEBS Lett 434:145-8. 1998
  2. ncbi Deletion of cysteine 369 in lysyl hydroxylase 1 eliminates enzyme activity and causes Ehlers-Danlos syndrome type VI
    H N Yeowell
    Division of Dermatology, Duke University Medical Center, Durham, NC 27710, USA
    Matrix Biol 19:37-46. 2000
  3. ncbi Characterization of three fragments that constitute the monomers of the human lysyl hydroxylase isoenzymes 1-3. The 30-kDa N-terminal fragment is not required for lysyl hydroxylase activity
    Kati Rautavuoma
    Collagen Research Unit, Biocenter Oulu and Department of Medical Biochemistry and Molecular Biology, University of Oulu, FIN 90014 Oulu, Finland
    J Biol Chem 277:23084-91. 2002
  4. ncbi Effect of hyper- and microgravity on collagen post-translational controls of MC3T3-E1 osteoblasts
    Mitsuru Saito
    Department of Orthopaedic Surgery, Jikei University School of Medicine, Tokyo, Japan
    J Bone Miner Res 18:1695-705. 2003
  5. ncbi An Ehlers-Danlos syndrome type VIA patient with cystic malformations of the meninges
    Heather N Yeowell
    Div of Dermatology, Duke Univ Medical Centre, Durham, NC, USA
    Eur J Dermatol 15:353-8. 2005
  6. ncbi Interleukin 4 and prolonged hypoxia induce a higher gene expression of lysyl hydroxylase 2 and an altered cross-link pattern: important pathogenetic steps in early and late stage of systemic scleroderma?
    J Brinckmann
    Department of Dermatology, University of Lubeck, Ratzeburger Allee 160, 23538 Lubeck, Germany
    Matrix Biol 24:459-68. 2005
  7. doi A new Ehlers-Danlos syndrome with craniofacial characteristics, multiple congenital contractures, progressive joint and skin laxity, and multisystem fragility-related manifestations
    Tomoki Kosho
    Department of Medical Genetics, Shinshu University School of Medicine, Matsumoto, Japan
    Am J Med Genet A 152:1333-46. 2010
  8. ncbi Differential diagnosis of muscular hypotonia in infants: the kyphoscoliotic type of Ehlers-Danlos syndrome (EDS VI)
    Uluc Yis
    Division of Child Neurology, Dokuz Eylul University School of Medicine, Department of Pediatrics, 35340 Izmir, Turkey
    Neuromuscul Disord 18:210-4. 2008
  9. ncbi Interstitial collagen synthesis and processing in human amnion: a property of the mesenchymal cells
    M L Casey
    Cecil H and Ida Green Center for Reproductive Biology Sciences, University of Texas, Southwestern Medical Center, Dallas 75235, USA
    Biol Reprod 55:1253-60. 1996
  10. ncbi Heterogeneous basis of the type VIB form of Ehlers-Danlos syndrome (EDS VIB) that is unrelated to decreased collagen lysyl hydroxylation
    L C Walker
    Division of Dermatology, Duke University Medical Center, Durham, North Carolina 27710, USA
    Am J Med Genet A 131:155-62. 2004

Research Grants

  1. Collagen remodeling and tumor progression
    Valerie Marie Weaver; Fiscal Year: 2013
  2. Collagen Glycosylation, Maturation and Mineralization
    Mitsuo Yamauchi; Fiscal Year: 2013
  3. SKIN REACTION TO LIGHT--NORMAL, PHOTOSENSITIVITY, CANCER
    MADHUKAR PATHAK; Fiscal Year: 1992
  4. MOLECULAR AND BIOCHEMICAL STUDY OF COLLAGEN IN PROLAPSE
    Anthony Visco; Fiscal Year: 2002
  5. COLLAGEN BIOSYNTHESIS IN HUMAN SKIN FIBROBLASTS
    Sheldon Pinnell; Fiscal Year: 1999
  6. LUNG COLLAGEN CROSSLINKING--BIOSYNTHESIS AND MATURATION
    Jerold Last; Fiscal Year: 1992
  7. REGULATION OF COLLAGEN MATRIX MINERALIZATION
    Mitsuo Yamauchi; Fiscal Year: 2007
  8. Phase 3A of a 3-phase 1.3-GHz LTS/HTS NMR magnet
    YUKIKAZU NA IWASA; Fiscal Year: 2013
  9. CHARACTERIZATION OF MEMBRANE PROTEIN BY SSNMR
    Ann McDermott; Fiscal Year: 2001
  10. Structural Studies of Photosynthetic Membrane Proteins
    MATTHEW CRADDOCK; Fiscal Year: 2007

Detail Information

Publications267 found, 100 shown here

  1. ncbi Identification of arginine-700 as the residue that binds the C-5 carboxyl group of 2-oxoglutarate in human lysyl hydroxylase 1
    K Passoja
    Biocenter and Department of Medical Biochemistry, University of Oulu, Finland
    FEBS Lett 434:145-8. 1998
    b>Lysyl hydroxylase catalyzes the formation of hydroxylysine in collagens by a reaction that involves oxidative decarboxylation of 2-oxoglutarate...
  2. ncbi Deletion of cysteine 369 in lysyl hydroxylase 1 eliminates enzyme activity and causes Ehlers-Danlos syndrome type VI
    H N Yeowell
    Division of Dermatology, Duke University Medical Center, Durham, NC 27710, USA
    Matrix Biol 19:37-46. 2000
    This study describes the relative contribution of the 10 cysteine residues in lysyl hydroxylase 1 (LH1) to enzyme activity...
  3. ncbi Characterization of three fragments that constitute the monomers of the human lysyl hydroxylase isoenzymes 1-3. The 30-kDa N-terminal fragment is not required for lysyl hydroxylase activity
    Kati Rautavuoma
    Collagen Research Unit, Biocenter Oulu and Department of Medical Biochemistry and Molecular Biology, University of Oulu, FIN 90014 Oulu, Finland
    J Biol Chem 277:23084-91. 2002
    b>Lysyl hydroxylase (LH) catalyzes the formation of hydroxylysine in collagens; three human isoenzymes have been cloned so far...
  4. ncbi Effect of hyper- and microgravity on collagen post-translational controls of MC3T3-E1 osteoblasts
    Mitsuru Saito
    Department of Orthopaedic Surgery, Jikei University School of Medicine, Tokyo, Japan
    J Bone Miner Res 18:1695-705. 2003
    ..We found that altered gravitational load affected the post-translational modification of collagen, particularly the collagen maturation pathway, through altered expression of enzymes involved in cross-link formation...
  5. ncbi An Ehlers-Danlos syndrome type VIA patient with cystic malformations of the meninges
    Heather N Yeowell
    Div of Dermatology, Duke Univ Medical Centre, Durham, NC, USA
    Eur J Dermatol 15:353-8. 2005
    ..9 kb in the lysyl hydroxylase 1 (LH1) gene that is the cause of severely decreased levels of LH activity in her skin fibroblasts...
  6. ncbi Interleukin 4 and prolonged hypoxia induce a higher gene expression of lysyl hydroxylase 2 and an altered cross-link pattern: important pathogenetic steps in early and late stage of systemic scleroderma?
    J Brinckmann
    Department of Dermatology, University of Lubeck, Ratzeburger Allee 160, 23538 Lubeck, Germany
    Matrix Biol 24:459-68. 2005
    ..In the present study, we analyzed the cross-link pattern and the gene expression of lysyl hydroxylase 2 (LH2) in the skin of SSc...
  7. doi A new Ehlers-Danlos syndrome with craniofacial characteristics, multiple congenital contractures, progressive joint and skin laxity, and multisystem fragility-related manifestations
    Tomoki Kosho
    Department of Medical Genetics, Shinshu University School of Medicine, Matsumoto, Japan
    Am J Med Genet A 152:1333-46. 2010
    ..and skeletal features, overlapping with the kyphoscoliosis type Ehlers-Danlos syndrome (EDS) but without lysyl hydroxylase deficiency [Kosho et al. (2005) Am J Med Genet Part A 138A:282-287]...
  8. ncbi Differential diagnosis of muscular hypotonia in infants: the kyphoscoliotic type of Ehlers-Danlos syndrome (EDS VI)
    Uluc Yis
    Division of Child Neurology, Dokuz Eylul University School of Medicine, Department of Pediatrics, 35340 Izmir, Turkey
    Neuromuscul Disord 18:210-4. 2008
    ..Biochemically, it is characterized by a deficiency of collagen lysyl hydroxylase (EC 1.14.11.4) due to mutations in PLOD1...
  9. ncbi Interstitial collagen synthesis and processing in human amnion: a property of the mesenchymal cells
    M L Casey
    Cecil H and Ida Green Center for Reproductive Biology Sciences, University of Texas, Southwestern Medical Center, Dallas 75235, USA
    Biol Reprod 55:1253-60. 1996
    ..2(I), and alpha 1(III) subunit mRNAs and the specific activities of the enzymes prolyl 4-hydroxylase and lysyl hydroxylase were greatest in human amnion tissue early in gestation, decreasing rather abruptly after 12-14 wk gestation ..
  10. ncbi Heterogeneous basis of the type VIB form of Ehlers-Danlos syndrome (EDS VIB) that is unrelated to decreased collagen lysyl hydroxylation
    L C Walker
    Division of Dermatology, Duke University Medical Center, Durham, North Carolina 27710, USA
    Am J Med Genet A 131:155-62. 2004
    ..diagnosis of Ehlers-Danlos syndrome type VI (EDS VI; kyphoscoliosis type), have significantly decreased lysyl hydroxylase (LH) activity due to mutations in the LH1 gene (classified as EDS VIA: OMIM no. 225400)...
  11. pmc Spondylocheiro dysplastic form of the Ehlers-Danlos syndrome--an autosomal-recessive entity caused by mutations in the zinc transporter gene SLC39A13
    Cecilia Giunta
    Division of Metabolism and Molecular Pediatrics, University Children s Hospital, CH 8032 Zurich, Switzerland
    Am J Hum Genet 82:1290-305. 2008
    ..2 in controls. Lysyl and prolyl residues of collagens were underhydroxylated despite normal lysyl hydroxylase and prolyl 4-hydroxylase activities; underhydroxylation was a generalized process as shown by mass ..
  12. ncbi Lysyl hydroxylase 2 is a specific telopeptide hydroxylase, while all three isoenzymes hydroxylate collagenous sequences
    Kati Takaluoma
    Collagen Research Unit, Biocenter Oulu and Department of Medical Biochemistry and Molecular Biology, University of Oulu, FIN 90014, Oulu, Finland
    Matrix Biol 26:396-403. 2007
    b>Lysyl hydroxylase (LH), with three isoenzymes in vertebrates, catalyzes the formation of hydroxylysine by acting on -X-Lys-Gly- triplets in the collagenous domains of proteins of the collagen superfamily and also in -X-Lys-Ala- or -X-Lys-..
  13. ncbi Mechanism of inhibition of lysyl hydroxylase activity by the organophosphates malathion and malaoxon
    A Samimi
    Pulmonary Critical Care Medicine, University of California, Davis, California 95616, USA
    Toxicol Appl Pharmacol 176:181-6. 2001
    Direct inhibition of lysyl hydroxylase by malathion and malaoxon was observed in an in vitro enzyme assay with recombinant lysyl hydroxylase expressed via a baculoviral system...
  14. pmc Premature aggregation of type IV collagen and early lethality in lysyl hydroxylase 3 null mice
    Kati Rautavuoma
    Collagen Research Unit and Department of Medical Biochemistry and Molecular Biology, Biocenter Oulu, University of Oulu, 90014 Oulu, Finland
    Proc Natl Acad Sci U S A 101:14120-5. 2004
    ..We generated mice with targeted inactivation of the gene for one of the three lysyl hydroxylase isoenzymes, LH3. The null embryos developed seemingly normally until embryonic day 8...
  15. ncbi Lysyl hydroxylase 3 (LH3) modifies proteins in the extracellular space, a novel mechanism for matrix remodeling
    Antti M Salo
    Department of Biochemistry and Biocenter Oulu, University of Oulu, Oulu, Finland
    J Cell Physiol 207:644-53. 2006
    b>Lysyl hydroxylase 3 (LH3), the multifunctional enzyme associated with collagen biosynthesis that possesses lysyl hydroxylase and collagen glycosyltransferase activities, has been characterized in the extracellular space in this study...
  16. ncbi Coenzyme A stimulates collagen production in cultured fibroblasts; possible mechanisms in enzymatic and gene expression
    K Mio
    Life Science Research Center, Lion Corporation, Odawara, Kanagawa, Japan
    Arch Dermatol Res 293:522-31. 2001
    ..hand, coenzyme A did not stimulate the activity nor the gene expression of two other processing enzymes: lysyl hydroxylase, which provides the sites for glycosylation and crosslinking between collagen peptides, and lysyl oxidase, a ..
  17. ncbi Procollagen synthesis is increased in hypothyroid rat ovary by a parallel and compensatory pathway
    Samir Kumar Saha
    Molecular Endocrinology Laboratory, Indian Institute of Chemical Biology, Kolkata, India
    Cell Physiol Biochem 19:313-22. 2007
    ..Down regulation of lysyl hydroxylase in hypothyroid condition results less collagen formation...
  18. ncbi Prolyl 3-hydroxylase 1, enzyme characterization and identification of a novel family of enzymes
    Janice A Vranka
    Research Department, Shriners Hospital for Children, Portland, Oregon 97239, USA
    J Biol Chem 279:23615-21. 2004
    ..3-hydroxylases, which share the conserved residues present in the active site of prolyl 4-hydroxylase and lysyl hydroxylase. P3H1 is the chick homologue of mammalian leprecan or growth suppressor 1...
  19. ncbi The myotomal diwanka (lh3) glycosyltransferase and type XVIII collagen are critical for motor growth cone migration
    Valerie A Schneider
    Department of Cell and Developmental Biology, University of Pennsylvania School of Medicine, Philadelphia, 19104, USA
    Neuron 50:683-95. 2006
    ..cloning of diwanka and show that it encodes LH3, a myotomally expressed multifunctional enzyme with lysyl hydroxylase and glycosyltransferase domains...
  20. ncbi Morphological and molecular analysis of idiopathic gingival fibromatosis: a case report
    Nicoletta Gagliano
    Department of Human Morphology LITA Segrate, University of Milan, Milan, Italy
    J Clin Periodontol 32:1116-21. 2005
    ..As this overgrowth involves collagen accumulation in the gingival connective tissue, we measured the collagen turnover to clarify the pathogenic mechanisms potentially involved...
  21. ncbi Lack of collagen type specificity for lysyl hydroxylase isoforms
    C Wang
    Department of Biochemistry, University of Oulu, Finland
    DNA Cell Biol 19:71-7. 2000
    b>Lysyl hydroxylase is the enzyme catalyzing the formation of hydroxylysyl residues in collagens. Large differences in the extent of hydroxylysyl residues are found among collagen types...
  22. pmc Cloning and characterization of a third human lysyl hydroxylase isoform
    K Passoja
    Collagen Research Unit, Biocenter and Department of Medical Biochemistry, University of Oulu, FIN 90220 Oulu, Finland
    Proc Natl Acad Sci U S A 95:10482-6. 1998
    b>Lysyl hydroxylase (EC 1.14.11.4), a homodimer, catalyzes the formation of hydroxylysine in collagens. Recently, an isoenzyme termed lysyl hydroxylase 2 has been cloned from human sources [M. Valtavaara, H. Papponen, A.-M. Pirttilä, K...
  23. ncbi Tissue specificity of a new splice form of the human lysyl hydroxylase 2 gene
    H N Yeowell
    Division of Dermatology, Duke University Medical Center, Durham, NC 27710, USA
    Matrix Biol 18:179-87. 1999
    In this study we present the first report of alternative RNA splicing in a gene for lysyl hydroxylase (LH) in a normal population. This splicing event, which we have observed in the LH2 gene, appears to be tissue specific...
  24. doi A therapeutic dose of FK506 does not affect collagen turnover pathways in healthy human gingival fibroblasts
    N Gagliano
    Department of Human Morphology, Extracellular Matrix Laboratory EML, University of Milan, Milan, Italy
    Transplant Proc 40:1419-24. 2008
    ..Collagen type I and III (COL-I, COL-III), lysyl hydroxylase 2b (LH2b), MMP-1 and -2, tissue inhibitor of MMP-1 and transforming growth factor-beta1 (TGF-beta1) mRNA ..
  25. ncbi Tissue-specific expression and regulation of the alternatively-spliced forms of lysyl hydroxylase 2 (LH2) in human kidney cells and skin fibroblasts
    Linda C Walker
    Division of Dermatology, Duke University Medical Center, Durham, Box 3135, NC 27710, USA
    Matrix Biol 23:515-23. 2005
    ..b>Lysyl hydroxylase 2 (LH2) exists as two alternatively-spliced forms; the long transcript (the major ubiquitously-expressed ..
  26. ncbi Effect of minoxidil on rabbit lens epithelial cell behavior in vitro and in situ
    I Ishida
    Department of Ophthalmology, Wakayama Medical University, Kimiidera, Japan
    Graefes Arch Clin Exp Ophthalmol 239:770-7. 2001
    ..Minoxidil is an inhibitor of lysyl hydroxylase, an enzyme involved in procollagen hydroxylation...
  27. ncbi Phenotypic and molecular characterization of Bruck syndrome (osteogenesis imperfecta with contractures of the large joints) caused by a recessive mutation in PLOD2
    Russia Ha-Vinh
    Division of Molecular Pediatrics, University of Lausanne, Lausanne, Switzerland
    Am J Med Genet A 131:115-20. 2004
    ..One BS locus has been mapped at 17p12, but more recently, two mutations in the lysyl hydroxylase 2 gene (PLOD2, 3q23-q24) have been identified in BS, showing genetic heterogeneity...
  28. ncbi Complete exon-intron organization of the gene for human lysyl hydroxylase 3 (LH3)
    K Rautavuoma
    Collagen Research Unit, Biocenter and Department of Medical Biochemistry, University of Oulu, Kajaanintie 52 A, FIN 90220, Oulu, Finland
    Matrix Biol 19:73-9. 2000
    b>Lysyl hydroxylase (LH) catalyzes the formation of hydroxylysine in collagens and related proteins by the hydroxylation of lysine residues in peptide linkages. Three isoenzymes of LH have so far been characterized...
  29. doi Differential effects of homocysteine and beta aminopropionitrile on preosteoblastic MC3T3-E1 cells
    Roman Thaler
    Ludwig Boltzmann Institute of Osteology, Hanusch Hospital, Vienna, Austria
    Bone 46:703-9. 2010
    ..increased the ratio of pyr/divalent cross-links of collagen, and down-regulated mRNA expression of lysyl hydroxylase (Plod2) and lysyl oxidase (Lox), genes which play an important role in the formation of a stable matrix...
  30. ncbi Effects of elevated circulating IGF-1 on the extracellular matrix in "high-growth" C57BL/6J mice
    K Reiser
    Department of Internal Medicine, School of Medicine, University of California, Davis 95616, USA
    Am J Physiol 271:R696-703. 1996
    ..Expression of types I and III collagen, lysyl oxidase, and lysyl hydroxylase was increased in all tissues analyzed...
  31. ncbi Evidence that impaired intracellular collagen synthesis reduces proliferation in cultured rat hepatocytes
    H Ikeda
    First Department of Internal Medicine, Faculty of Medicine, University of Tokyo, Japan
    Biochem Biophys Res Commun 200:1701-7. 1994
    ..The addition of P-1894B or minoxidil, which inhibits specifically prolyl hydroxylase or lysyl hydroxylase, respectively, also produced similar results...
  32. ncbi PITX2 isoform-specific regulation of atrial natriuretic factor expression: synergism and repression with Nkx2.5
    Mrudula Ganga
    Department of Biological Science, The University of Tulsa, Tulsa, Oklahoma 74104 3189, USA
    J Biol Chem 278:22437-45. 2003
    ..5. We further demonstrate that the procollagen lysyl hydroxylase (PLOD1) promoter is regulated by Nkx2.5. Mechanistically, PITX2C and Nkx2...
  33. ncbi Penicillin G does not alter collagen type I metabolism of dermal fibroblasts in culture
    U Anderegg
    Department of Dermatology, Leipzig University, and Saxon Academy of Science, Leipzig, Germany
    Dermatology 200:111-4. 2000
    ..Penicillin G (PenG) has successfully been used for therapy of dermal fibrosis in patients suffering from systemic sclerosis (SSc). However, there is little knowledge on the mechanism of the antifibrotic action of PenG...
  34. ncbi cDNA cloning and expression of bovine aspartyl (asparaginyl) beta-hydroxylase
    S Jia
    Merck Sharp and Dohme Research Laboratories, West Point, Pennsylvania 19486
    J Biol Chem 267:14322-7. 1992
    ..amino acid sequences of two other alpha-ketoglutarate-dependent dioxygenases, prolyl-4-hydroxylase and lysyl hydroxylase, with that of Asp beta-hydroxylase showed no significant homologies...
  35. pmc Genomic structure and embryonic expression of zebrafish lysyl hydroxylase 1 and lysyl hydroxylase 2
    Valerie A Schneider
    University of Pennsylvania School of Medicine, Department of Cell and Developmental Biology, Philadelphia, PA 19104 6058, USA
    Matrix Biol 26:12-9. 2007
    Collagen biosynthesis in both invertebrates and vertebrates is critically dependent upon the activity of lysyl hydroxylase (LH) enzymes...
  36. ncbi Cloning and characterization of a novel human lysyl hydroxylase isoform highly expressed in pancreas and muscle
    M Valtavaara
    Biocenter and Department of Biochemistry, University of Oulu, FIN 90570 Oulu, Finland
    J Biol Chem 272:6831-4. 1997
    We report the isolation and characterization of cDNA clones for a novel isoform of lysyl hydroxylase (lysyl hydroxylase 2), a posttranslational enzyme of collagen biosynthesis...
  37. pmc Lingual leiomyomatous hamartoma with bifid tip and ankyloglossia in a patient without oral-facial-digital syndrome: a case report and literature review
    Hsing Liang Wang
    Department of Otolaryngology Head and Neck Surgery, Faculty of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung City, Taiwan
    World J Surg Oncol 11:230. 2013
    Here is a rare case of lingual leiomyomatous hamartoma (LLH) with bifid tongue tip and tongue-tie in a patient with non-oral-facial-digital syndrome (OFDS)...
  38. ncbi Characterization of a mutant strain of Rhodovulum sulfidophilum lacking the pufA and pufB genes encoding the polypeptides for the light-harvesting complex 1 (B 870)
    Laura J Raiger-Iustman
    Catedra de Microbiologia, Facultad de Agronomia, UBA and Instituto de Investigaciones Bioquímicas y Fisiológicas IByF CONICET, Av San Martin 4453, 1417, Capital Federal, Argentina
    Arch Microbiol 185:407-15. 2006
    ..complex 2 (LH2) directly to the reaction center (RC) in mutant strains lacking the core light-harvesting complex 1 (LH1) have been obtained with cells of Rhodobacter capsulatus and Rhodobacter sphaeroides...
  39. pmc Projection structure of the photosynthetic reaction centre-antenna complex of Rhodospirillum rubrum at 8.5 A resolution
    Stuart J Jamieson
    Krebs Institute for Biomolecular Research, Department of Molecular Biology and Biotechnology, University of Sheffield, Firth Court, Western Bank, Sheffield S10 2TN, UK
    EMBO J 21:3927-35. 2002
    Two-dimensional crystals of the reaction-centre-light-harvesting complex I (RC-LH1) of the purple non- sulfur bacterium Rhodospirillum rubrum have been formed from detergent-solubilized and purified protein complexes...
  40. pmc Excitation dynamics of two spectral forms of the core complexes from photosynthetic bacterium Thermochromatium tepidum
    Fei Ma
    Beijing National Laboratory for Molecular Science, State Key Laboratory for Structural Chemistry of Unstable and Stable Species, Institute of Chemistry, Chinese Academy of Sciences, Beijing, China
    Biophys J 95:3349-57. 2008
    The intact core antenna-reaction center (LH1-RC) core complex of thermophilic photosynthetic bacterium Thermochromatium (Tch.) tepidum is peculiar in its long-wavelength LH1-Q(y) absorption (915 nm)...
  41. pmc Abnormal type I collagen post-translational modification and crosslinking in a cyclophilin B KO mouse model of recessive osteogenesis imperfecta
    Wayne A Cabral
    Bone and Extracellular Matrix Branch, NICHD, NIH, Bethesda, Maryland, United States of America
    PLoS Genet 10:e1004465. 2014
    ..We confirmed and extended the reported role of CyPB in supporting collagen lysyl hydroxylase (LH1) activity...
  42. doi Construction and structural analysis of tethered lipid bilayer containing photosynthetic antenna proteins for functional analysis
    Ayumi Sumino
    Department of Frontier Materials, Graduate School of Engineering, Nagoya Institute of Technology, Gokiso cho, Showa Ku, Nagoya 466 8555, Japan
    Biomacromolecules 12:2850-8. 2011
    ..bacterial photosynthetic membrane proteins, light-harvesting complex 2 (LH2), and light-harvesting core complex (LH1-RC) is described and establishes this system as an experimental platform for their functional analysis...
  43. pmc Rhabdoid tumor: gene expression clues to pathogenesis and potential therapeutic targets
    Samantha Gadd
    Department of Pathology, Northwestern University s Feinberg School of Medicine, Chicago, IL, USA
    Lab Invest 90:724-38. 2010
    ....
  44. ncbi Energy transfer and charge separation in the purple non-sulfur bacterium Roseospirillum parvum
    H P Permentier
    Department of Biophysics, Huygens Laboratory, Leiden University, The Netherlands
    Biochim Biophys Acta 1460:338-45. 2000
    ..6 ps. This and other evidence indicate that both types of BChl belong to a single LH1 complex. Flash induced difference spectra show that the primary electron donor absorbs at 886 nm, i.e...
  45. doi Natronovirga wadinatrunensis gen. nov., sp. nov. and Natranaerobius trueperi sp. nov., halophilic, alkalithermophilic micro-organisms from soda lakes of the Wadi An Natrun, Egypt
    Noha M Mesbah
    Department of Microbiology, University of Georgia, Athens, GA 30602, USA
    Int J Syst Evol Microbiol 59:2042-8. 2009
    ..nov. Strain JW/NM-WN-LH1T represents a novel genus and species within the family Natranaerobiaceae, Natronovirga wadinatrunensis gen. nov., sp. nov. The type strain of the type species is JW/NM-WN-LH1T (=DSM 18770T=ATCC BAA-1444T)...
  46. ncbi Evaluation of structure-function relationships in the core light-harvesting complex of photosynthetic bacteria by reconstitution with mutant polypeptides
    C M Davis
    Department of Biochemistry, Molecular Biology, and Cell Biology, Northwestern University, Evanston, Illinois 60208 3500, USA
    Biochemistry 36:3671-9. 1997
    Seven mutant LH1 polypeptides of Rhodobactor sphaeroides have been isolated, and their behaviors in in vitro reconstitution of LH1 and its subunit complex have been characterized...
  47. doi Reconstitution of bacterial photosynthetic unit in a lipid bilayer studied by single-molecule spectroscopy at 5 K
    Daisuke Uchiyama
    Department of Physics, Tokyo Institute of Technology, Tokyo 152 8551, Japan
    Phys Chem Chem Phys 13:11615-9. 2011
    ..a lipid bilayer by reconstitution of the light-harvesting 2 (LH2) complex and light-harvesting 1-reaction center (LH1-RC) complex through detergent removal of their micelles in the presence of lipids...
  48. ncbi Oxygen tension regulates the expression of a group of procollagen hydroxylases
    Karl Heinz Hofbauer
    Institut für Physiologie der Universität Regensburg, Germany
    Eur J Biochem 270:4515-22. 2003
    ..involved in collagen fiber formation, such as prolyl-4-hydroxylases (Ph4) and procollagen lysyl-hydroxylases (PLOD)...
  49. pmc Reduction of lysyl hydroxylase 3 causes deleterious changes in the deposition and organization of extracellular matrix
    Maija Risteli
    Department of Biochemistry, P O Box 3000, University of Oulu, FI 90014 Oulu, Finland
    J Biol Chem 284:28204-11. 2009
    b>Lysyl hydroxylase 3 (LH3) is a multifunctional enzyme possessing lysyl hydroxylase, collagen galactosyltransferase, and glucosyltransferase (GGT) activities...
  50. ncbi Mutational analysis of the lysyl hydroxylase 1 gene (PLOD) in six unrelated patients with Ehlers-Danlos syndrome type VI: prenatal exclusion of this disorder in one family
    H N Yeowell
    Duke University Medical Center, Durham, NC 27710, USA
    Hum Mutat 16:90. 2000
    Screening of full length cDNAs for lysyl hydroxylase 1 (LH1; also PLOD) amplified from dermal fibroblasts from six unrelated patients with the autosomal recessive disorder Ehlers-Danlos syndrome type VI (EDS VI) has shown them to be both ..
  51. pmc A lysine-rich motif in the phosphatidylserine receptor PSR-1 mediates recognition and removal of apoptotic cells
    Hengwen Yang
    Department of Molecular, Cellular and Developmental Biology, University of Colorado, Boulder, Colorado 80309, USA
    Nat Commun 6:5717. 2015
    ..However, several studies suggest that PSR may also act as an arginine demethylase, a lysyl hydroxylase, or an RNA-binding protein through its N-terminal JmjC domain...
  52. ncbi Probing binding site of bacteriochlorophyll a and carotenoid in the reconstituted LH1 complex from Rhodospirillum rubrum S1 by Stark spectroscopy
    Katsunori Nakagawa
    Department of Applied Chemistry, Graduate School of Engineering, Nagoya Institute of Technology, Gokiso cho, Nagoya, 466 8555, Japan
    Photosynth Res 95:339-44. 2008
    ..Tr(Deltaalpha) and static dipole-moment change |Deltamu| upon photoexcitation) of isolated and of reconstituted LH1 complexes from the purple photosynthetic bacterium, Rhodospirillum (Rs.) rubrum...
  53. ncbi Lysyl hydroxylase-2b directs collagen cross-linking pathways in MC3T3-E1 cells
    Suchaya Pornprasertsuk
    Dental Research Center, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599 7455, USA
    J Bone Miner Res 19:1349-55. 2004
    ..The data indicate that LH2b directs collagen cross-linking pathways through its action on telopeptidyl lysine residues...
  54. ncbi Atomic force microscopy reveals multiple patterns of antenna organization in purple bacteria: implications for energy transduction mechanisms and membrane modeling
    James N Sturgis
    Laboratoire d Ingenierie des Systemes Macromoleculaires, Institute de Biologie Structurale et Microbiologie, UPR 9027, CNRS, 31 Chemin Joseph Aiguier, Marseille, 13402, France
    Photosynth Res 95:269-78. 2008
    ..LH2 antenna was seen either in large clusters or in fixed rows interspersed among ordered arrays of dimeric LH1-reaction center (RC) core complexes...
  55. ncbi Monitoring response to treatment in melanoma patients: potential of a serum glycomic marker
    Senthamil R Selvan
    Cell Biology Laboratory, Hoag Cancer Center, Newport Beach, CA 92663, USA
    Int J Cancer 122:1374-83. 2008
    ..volunteers on weeks 0, 4 and 24, patients were categorized into cohorts-I (LLL, n = 16), II (HHL/HLL, n = 4), III (LLH/LHH/LHL, n = 7) and IV (HHH/HLH, n = 7)...
  56. pmc Regulation of the collagen cross-linking enzymes LOXL2 and PLOD2 by tumor-suppressive microRNA-26a/b in renal cell carcinoma
    Akira Kurozumi
    Department of Functional Genomics, Chiba University Graduate School of Medicine, Chiba 260 8670, Japan
    Int J Oncol 48:1837-46. 2016
    ..Thus, our data showed that two genes promoting metastasis, LOXL2 and PLOD2, were epigenetically regulated by tumor-suppressive microRNAs, miR-26a and miR-26b, providing important insights into the molecular mechanisms of RCC metastasis...
  57. ncbi Expression profile of proteins involved in scar formation in the healing process of full-thickness excisional wounds in the porcine model
    Magda M W Ulrich
    Association of Dutch Burn Centres, Beverwijk, The Netherlands
    Wound Repair Regen 15:482-90. 2007
    ..This type of cross-linking is dependent on the enzyme lysyl hydroxylase-2b...
  58. doi Ciliate ingestion and digestion: flow cytometric measurements and regrowth of a digestion-resistant Campylobacter jejuni
    Matthew R First
    Department of Geology and Geophysics, Woods Hole Oceanographic Institution, Woods Hole, Massachusetts 02543, USA
    J Eukaryot Microbiol 59:12-9. 2012
    ..Campylobacter and the nonpathogenic bacterium Pseudomonas putida LH1 were labeled with a 5-chloromethylfluorescein diacetate, which fluoresces in intact and active cells but fades when ..
  59. doi Maternal dietary n-6/n-3 fatty acid ratio affects type 1 diabetes development in the offspring of non-obese diabetic mice
    Yukiko Kagohashi
    Department of Health and Nutrition, The University of Shimane, Matsue, Shimane, Japan
    Congenit Anom (Kyoto) 50:212-20. 2010
    ..combined L and H chows during gestation, lactation in mother and in post-weaning offspring revealed that only the LLH chow significantly suppressed the development of diabetes with similar kinetics to LLL chow, although the other ..
  60. ncbi Investigation of Rhodobacter capsulatus PufX interactions in the core complex of the photosynthetic apparatus
    Muktak Aklujkar
    Department of Microbiology and Immunology, University of British Columbia, V6T 1Z3, Vancouver, BC, Canada
    Photosynth Res 88:159-71. 2006
    ..the genus Rhodobacter includes a core complex consisting of the reaction centre (RC), light-harvesting complex 1 (LH1), and the PufX protein. PufX modulates LH1 structure and facilitates photosynthetic quinone/quinol exchange...
  61. ncbi Crystal structure of the 2-oxoglutarate- and Fe(II)-dependent lysyl hydroxylase JMJD6
    Monica Mantri
    Department of Chemistry and Oxford Centre for Integrative Systems Biology, Chemistry Research Laboratory, University of Oxford, 12 Mansfield Road, Oxford OX1 3TA, UK
    J Mol Biol 401:211-22. 2010
    ..Together with mutational studies, the structural data suggest how JMJD6 binds its lysyl residues such that it can catalyse C-5 hydroxylation rather than Nepsilon-demethylation, as for analogous enzymes...
  62. pmc Lysyl Hydroxylase 3 Localizes to Epidermal Basement Membrane and Is Reduced in Patients with Recessive Dystrophic Epidermolysis Bullosa
    Stephen A Watt
    Division of Cancer Research, University of Dundee, Dundee, United Kingdom
    PLoS ONE 10:e0137639. 2015
    ..identify a significant decrease in PLOD3 expression and its encoded protein, the collagen modifying enzyme lysyl hydroxylase 3 (LH3), in RDEB...
  63. doi Probing the effect of the binding site on the electrostatic behavior of a series of carotenoids reconstituted into the light-harvesting 1 complex from purple photosynthetic bacterium Rhodospirillum rubrum detected by stark spectroscopy
    Katsunori Nakagawa
    Department of Life and Materials Engineering, Graduate School of Engineering, Nagoya Institute of Technology, Nagoya, Japan
    J Phys Chem B 112:9467-75. 2008
    Reconstitutions of the LH1 complexes from the purple photosynthetic bacterium Rhodospirillum rubrum S1 were performed with a range of carotenoid molecules having different numbers of C=C conjugated double bonds...
  64. doi The origin of the unusual Qy red shift in LH1-RC complexes from purple bacteria Thermochromatium tepidum as revealed by Stark absorption spectroscopy
    Fei Ma
    Department of Biophysics, Faculty of Sciences, VU University Amsterdam, De Boelelaan 1081, 1081 HV Amsterdam, The Netherlands Electronic address
    Biochim Biophys Acta 1847:1479-86. 2015
    Native LH1-RC of photosynthetic purple bacteria Thermochromatium (Tch.) tepidum, B915, has an ultra-red BChl a Qy absorption...
  65. ncbi Platelet factor 4 gene transfection into tumor cells inhibits angiogenesis, tumor growth and metastasis
    Kentaro Yamaguchi
    Department of Surgery, Tokyo Women s Medical University Daini Hospital, 2 1 10 Nishiogu, Arakawa ku Tokyo, 116 8567, Japan
    Anticancer Res 25:847-51. 2005
    ..In this study, Lewis lung carcinoma (LLH) cells were transfected with the human PF4 via mammalian expression vectors and the ability of the transfected ..
  66. doi From the left to the right: 13-year experience in laparoscopic living donor liver transplantation
    Raffaele Brustia
    Department of Hepatobiliary Surgery and Liver Transplantation, Hopital Pitie Salpetriere, Assistance Publique Hopitaux de Paris, 47 83 boulevard de l Hopital, 75013, Paris, France
    Updates Surg 67:193-200. 2015
    ..Laparoscopic Left Lateral Section (LLLS) was performed in children receiver, while Laparoscopic Left Hepatectomy (LLH)-including or not the middle hepatic vein-and Laparoscopic Right Hepatectomy (LRH) in adults...
  67. ncbi In vivo carotenoid triplet formation in response to excess light: a supramolecular photoprotection mechanism revisited
    Jean Alric
    Laboratoire de Genetique et Biophysique des Plantes, UMR, 6191 CNRS CEA Aix Marseille II, France
    Photosynth Res 83:335-41. 2005
    ..work, we investigate the coupling between energy conversion and energy deactivation, in isolated complexes of RC-LH1 and LH2 increasingly integrated systems up to intact cells of the purple anaerobic photosynthetic bacterium ..
  68. ncbi Crystal structure of the RC-LH1 core complex from Rhodopseudomonas palustris
    Aleksander W Roszak
    Department of Chemistry, Institute of Biomedical and Life Sciences, University of Glasgow, Glasgow G12 8QQ, UK
    Science 302:1969-72. 2003
    The crystal structure at 4.8 angstrom resolution of the reaction center-light harvesting 1 (RC-LH1) core complex from Rhodopseudomonas palustris shows the reaction center surrounded by an oval LH1 complex that consists of 15 pairs of ..
  69. pmc Missense mutations that cause Bruck syndrome affect enzymatic activity, folding, and oligomerization of lysyl hydroxylase 2
    Marjo Hyry
    Oulu Center for Cell Matrix Research, Biocenter Oulu, and Department of Medical Biochemistry and Molecular Biology, University of Oulu, FIN 90014 Oulu, Finland
    J Biol Chem 284:30917-24. 2009
    ..Three point mutations in lysyl hydroxylase (LH) 2, the enzyme responsible for the hydroxylation of collagen telopeptides, have been identified in Bruck ..
  70. ncbi Identification of amino acids important for the catalytic activity of the collagen glucosyltransferase associated with the multifunctional lysyl hydroxylase 3 (LH3)
    Chunguang Wang
    Department of Biochemistry, Biocenter Oulu, University of Oulu, P O Box 3000, University of Oulu, FIN 90014, Finland
    J Biol Chem 277:18568-73. 2002
    Collagen glucosyltransferase (GGT) activity has recently been shown to be associated with human lysyl hydroxylase (LH) isoform 3 (LH3) (Heikkinen, J., Risteli, M., Wang, C., Latvala, J., Rossi, M., Valtavaara, M., Myllylä, R. (2000) J...
  71. pmc A connective tissue disorder caused by mutations of the lysyl hydroxylase 3 gene
    Antti M Salo
    Department of Biochemistry, University of Oulu, PO Box 3000, FI 90014 University of Oulu, Finland
    Am J Hum Genet 83:495-503. 2008
    b>Lysyl hydroxylase 3 (LH3, encoded by PLOD3) is a multifunctional enzyme capable of catalyzing hydroxylation of lysyl residues and O-glycosylation of hydroxylysyl residues producing either monosaccharide (Gal) or disaccharide (Glc-Gal) ..
  72. doi A case of Ehlers-Danlos syndrome type VIA with a novel PLOD1 gene mutation
    Ayse Tosun
    Division of Pediatric Neurology, Department of Pediatrics, Faculty of Medicine, Adnan Menderes University, Aydin, Turkey Electronic address
    Pediatr Neurol 51:566-9. 2014
    ..The kyphoscoliotic type of the Ehlers-Danlos syndrome is an autosomal recessive connective tissue disorder characterized by soft extensible skin, laxity of joints, severe muscle hypotonia at birth, and kyphoscoliosis...
  73. ncbi Lysyl hydroxylase 3 is a multifunctional protein possessing collagen glucosyltransferase activity
    J Heikkinen
    Department of Biochemistry, University of Oulu, P O Box 3000, FIN 90014 Oulu, Finland
    J Biol Chem 275:36158-63. 2000
    b>Lysyl hydroxylase (EC ) and glucosyltransferase (EC ) are enzymes involved in post-translational modifications during collagen biosynthesis...
  74. ncbi Vitamin C and bone markers: investigations in a Gambian population
    Karen Munday
    Medical Research Council Human Nutrition Research, Elsie Widdowson Laboratory, Fulbourn Road, Cambridge CB1 9NL, UK
    Proc Nutr Soc 62:429-36. 2003
    ..Vitamin C acts as a cofactor for the enzyme lysyl hydroxylase, which is required for the hydroxylation of lysine residues in procollagen chains...
  75. doi Collagen cross-links as a determinant of bone quality: a possible explanation for bone fragility in aging, osteoporosis, and diabetes mellitus
    M Saito
    Department of Orthopaedic Surgery, Jikei University School of Medicine, 3 25 8 Nishi shinbashi, Minato ku, Tokyo 105 8461, Japan
    Osteoporos Int 21:195-214. 2010
    ..Collagen cross-links can be divided into lysyl hydroxylase and lysyloxidase-mediated enzymatic immature divalent cross-links,mature trivalent pyridinoline and pyrrole ..
  76. pmc Glycosylation and cross-linking in bone type I collagen
    Masahiko Terajima
    From the North Carolina Oral Health Institute, School of Dentistry, University of North Carolina, Chapel Hill, North Carolina 27599
    J Biol Chem 289:22636-47. 2014
    ..Perdivara, I., Yokoyama, M., Nagaoka, H., Terajima, M., Tomer, K. B., and Yamauchi, M. (2012) Lysyl hydroxylase 3-mediated glucosylation in type I collagen: molecular loci and biological significance. J. Biol. Chem...
  77. doi Excitation energy transfer and trapping dynamics in the core complex of the filamentous photosynthetic bacterium Roseiflexus castenholzii
    Yueyong Xin
    Departments of Biology and Chemistry, Washington University, St Louis, MO 63130, USA
    Photosynth Res 111:149-56. 2012
    ..Assuming that the light harvesting complex is generally similar to LH1 of purple bacteria, the possible structural and functional aspects of this unique antenna complex are discussed...
  78. doi Ca(2+)-binding reduces conformational flexibility of RC-LH1 core complex from thermophile Thermochromatium tepidum
    Selma Jakob-Grun
    Department Biology I, Botany, Ludwig Maximilians University Munich, 82152, Planegg Martinsried, Germany
    Photosynth Res 111:139-47. 2012
    The light-harvesting complex, LH1, of thermophile purple bacteria Thermochromatium tepidum consists of an array of α- and β-polypeptides which assemble the photoactive bacteriochlorophyll and closely interact with the membrane-lipids...
  79. ncbi Molecular cloning of chick lysyl hydroxylase. Little homology in primary structure to the two types of subunit of prolyl 4-hydroxylase
    R Myllyla
    Collagen Research Unit, University of Oulu, Finland
    J Biol Chem 266:2805-10. 1991
    b>Lysyl hydroxylase (EC 1.14.11.4), an alpha 2 dimer, catalyzes the formation of hydroxylysine in collagens by the hydroxylation of lysine residues in X-Lys-Gly sequences...
  80. ncbi Isolation, size estimates, and spectral heterogeneity of an oligomeric series of light-harvesting 1 complexes from Rhodobacter sphaeroides
    Willem H J Westerhuis
    Department of Molecular Biology and Biochemistry, Rutgers University, Piscataway, New Jersey 08854 8082, USA
    Biochemistry 41:8698-707. 2002
    A series of light-harvesting 1 (LH1) complexes was isolated by lithium dodecyl sulfate-polyacrylamide gel electrophoresis at 4 degrees C from Rhodobacter sphaeroides M21, which lacks the peripheral light-harvesting 2 (LH2) complex...
  81. pmc Excited state dynamics in photosynthetic reaction center and light harvesting complex 1
    Johan Strumpfer
    Center for Biophysics and Computational Biology, University of Illinois at Urbana Champaign, Urbana, Illinois 61801, USA
    J Chem Phys 137:065101. 2012
    ..the purple photosynthetic bacterium Rhodobacter sphaeroides studied here, surrounded by light harvesting complex 1 (LH1)...
  82. ncbi Polarization angle dependence of stark absorption spectra of spirilloxanthin bound to the reconstituted LH1 complexes using LH1-subunits isolated from the purple photosynthetic bacterium Rhodospirillum rubrum
    Tomoko Horibe
    Department of Physics, Graduate School of Science, Osaka City University, Sugimoto, Sumiyoshi ku, Japan
    Acta Biochim Pol 59:97-100. 2012
    Reconstituted LH1 complexes were prepared using the LH1 subunit-type complexes, isolated from the purple photosynthetic bacterium Rhodospirillum (Rs.) rubrum, and purified all-trans spirilloxanthin...
  83. ncbi Dynamics of excitation energy transfer in the LH1 and LH2 light-harvesting complexes of photosynthetic bacteria
    R van Grondelle
    Department of Biophysics and Physics of Complex Systems, Faculty of Sciences, Vrije Universiteit, De Boelelaan 1081, 1081 HV Amsterdam, The Netherlands
    Biochemistry 40:15057-68. 2001
    ..We are now in a position to discuss the physical origins of excitation energy transfer and trapping in the LH2 and LH1 antennae of photosynthetic purple bacteria...
  84. doi Differential effects of bone morphogenetic protein-2 and transforming growth factor-beta1 on gene expression of collagen-modifying enzymes in human adipose tissue-derived mesenchymal stem cells
    Marlene Knippenberg
    Department of Oral Cell Biology, Academic Centre of Dentistry Amsterdam ACTA, Universiteit van Amsterdam, and Research Institute MOVE, Vrije Universiteit, Amsterdam, The Netherlands
    Tissue Eng Part A 15:2213-25. 2009
    ..AT-MSCs may thus be able to appropriately modify type I collagen to form a functional bone extracellular matrix for tissue engineering, dependent on the growth factor added...
  85. ncbi Combination of Genistein and Cisplatin with Two Designed Monofunctional Platinum Agents in Human Ovarian Tumour Models
    Laila Arzuman
    Discipline of Biomedical Science, Sydney Medical School, The University of Sydney, Cumberland Campus, Lidcombe, NSW, Australia
    Anticancer Res 35:6027-39. 2015
    ..we report the synthesis of monofunctional platinum tris(3-hydroxypyridine)chloroplatinum(II) chloride (coded as LH1) and tris(imidazole)chloroplatinum(II) chloride (coded as LH2), and their activity alone and in combination with ..
  86. doi Discovery and characterization of hydroxylysine in recombinant monoclonal antibodies
    Qing Xie
    a Protein Analytical Chemistry Department, Genentech, 1 DNA Way, 94080, South San Francisco, CA, USA
    MAbs 8:371-8. 2016
    ..proteins such as collagen, and was therefore presumed to result from the activity of the CHO homolog of the lysyl hydroxylase complex...
  87. ncbi Role of the C-terminal extrinsic region of the alpha polypeptide of the light-harvesting 2 complex of Rhodobacter sphaeroides: a domain swap study
    John D Olsen
    Robert Hill Institute for Photosynthesis, Department of Molecular Biology and Biotechnology, University of Sheffield, Western Bank, United Kingdom
    Biochemistry 42:15114-23. 2003
    The LH1 and LH2 complexes of Rhodobacter sphaeroides form ring structures of 16 and 9 protomers, respectively, comprising alpha and beta polypeptides, bacteriochlorophylls (Bchl), and carotenoids...
  88. doi Ultra-broadband 2D electronic spectroscopy of carotenoid-bacteriochlorophyll interactions in the LH1 complex of a purple bacterium
    Margherita Maiuri
    CNR IFN, Dipartimento di Fisica, Politecnico di Milano, P zza L Da Vinci 32, Milano 20133, Italy
    J Chem Phys 142:212433. 2015
    We investigate the excitation energy transfer (EET) pathways in the photosynthetic light harvesting 1 (LH1) complex of purple bacterium Rhodospirillum rubrum with ultra-broadband two-dimensional electronic spectroscopy (2DES)...
  89. ncbi Self-assembled monolayer of light-harvesting core complexes from photosynthetic bacteria on a gold electrode modified with alkanethiols
    Masaharu Kondo
    Department of Applied Chemistry, Nagoya Institute of Technology, Gokiso cho, Showa Ku, Nagoya 466 8555, Japan
    Biomacromolecules 8:2457-63. 2007
    Light-harvesting antenna core (LH1-RC) complexes isolated from Rhodoseudomonas palustris were self-assembled on a gold electrode modified with self-assembled monolayers (SAMs) of the alkanethiols NH2(CH2)nSH, n = 2, 6, 8, 11; HOOC(CH2)..
  90. doi Rapid detection of gene mutations responsible for non-syndromic aortic aneurysm and dissection using two different methods: resequencing microarray technology and next-generation sequencing
    Haruya Sakai
    Department of Human Genetics, Yokohama City University Graduate School of Medicine, Kanazawa Ku, Yokohama, Japan
    Hum Genet 131:591-9. 2012
    ..Next-generation sequencing was able to detect almost all types of mutation, but requires improved informatics methods...
  91. ncbi Increased formation of pyridinoline cross-links due to higher telopeptide lysyl hydroxylase levels is a general fibrotic phenomenon
    Annemarie J van der Slot
    TNO Prevention and Health, Biomedical Research Division, Department of Tissue Repair, PO Box 2215, 2301 CE Leiden, The Netherlands
    Matrix Biol 23:251-7. 2004
    ..This is consistent with our previous observation that LH2b is a telopeptide lysyl hydroxylase. We conclude that the increased expression of LH2b, leading to the increased formation of pyridinoline cross-..
  92. doi The nature of coherences in the B820 bacteriochlorophyll dimer revealed by two-dimensional electronic spectroscopy
    Marco Ferretti
    VU University, Amsterdam, The Netherlands
    Phys Chem Chem Phys 16:9930-9. 2014
    ..2DES to an excitonically coupled bacteriochlorophyll dimer, the B820 subunit of the light harvesting complex 1 (LH1-RC) of R. rubrum G9...
  93. ncbi A translocation interrupts the COL5A1 gene in a patient with Ehlers-Danlos syndrome and hypomelanosis of Ito
    H V Toriello
    Cytogenetics Laboratory, Butterworth Hospital, Grand Rapids, Michigan 49503, USA
    Nat Genet 13:361-5. 1996
    ..types I and III have been demonstrated in EDS types VII and IV, respectively, while mutations in the lysyl hydroxylase and ATP7A genes, with roles in collagen cross-linking, are responsible for EDS types VI and IX...
  94. ncbi Hydrophobic pockets at the membrane interface: an original mechanism for membrane protein interactions
    Veronique Arluison
    Service de Biophysique des Fonctions Membranaires, DBJC CEA and URA 2096 CNRS, CE Saclay, 91191 Gif Yvette cedex, France
    Biochemistry 43:1276-82. 2004
    The effect of partial digestion by trypsin and GluC protease on the association of the membrane polypeptides of LH1 from Rhodospirillum (Rsp.) rubrum was studied...
  95. pmc Nanodissection and high-resolution imaging of the Rhodopseudomonas viridis photosynthetic core complex in native membranes by AFM. Atomic force microscopy
    Simon Scheuring
    Institut Curie, Unité Mixte de Recherche Centre National de la Recherche Scientifique 168 and Laboratoire de Recherche Correspondant Commissariat à l Energie Atomique 34V, 11 rue Pierre et Marie Curie, 75231 Paris Cedex 05, France
    Proc Natl Acad Sci U S A 100:1690-3. 2003
    ..vertical resolution reveal a single reaction center (RC) surrounded by a closed ellipsoid of 16 light-harvesting (LH1) subunits...
  96. doi Monomeric RC-LH1 core complexes retard LH2 assembly and intracytoplasmic membrane formation in PufX-minus mutants of Rhodobacter sphaeroides
    Peter G Adams
    Department of Molecular Biology and Biochemistry, University of Sheffield, United Kingdom
    Biochim Biophys Acta 1807:1044-55. 2011
    ..of light-harvesting 2 (LH2) complexes surround and interconnect dimeric reaction centre-light-harvesting 1-PufX (RC-LH1-PufX) 'core' complexes, forming extensive networks for energy transfer and trapping...
  97. ncbi Secretion and assembly of type IV and VI collagens depend on glycosylation of hydroxylysines
    Laura Sipilä
    Biocenter Oulu, Department of Biochemistry, University of Oulu, FI 90014 Oulu, Finland
    J Biol Chem 282:33381-8. 2007
    ..The formation of glycosylated hydroxylysines is catalyzed by multifunctional lysyl hydroxylase 3 (LH3) in vivo, and we have used LH3-manipulated mice and cells as models to study the function of these ..
  98. ncbi Reaction mechanism of CO oxidation on Cu(2)O(111): A density functional study
    Bao Zhen Sun
    College of Chemistry and Chemical Engineering, Fuzhou University, Fuzhou 350108, People s Republic of China
    J Chem Phys 133:154502. 2010
    ..Four possible mechanisms are explored (denoted as M(ER1), M(ER2), M(LH1), and M(LH2), respectively): M(ER1) is CO((gas))+O(2(ads))→CO(2(gas)); M(ER2) is CO((gas))+O(2(ads))→CO(3(ads))..
  99. ncbi Spectroscopic characterisation of a tetrameric subunit form of the core antenna protein from Rhodospirillum rubrum
    Attila P Vegh
    Section de Biophysique des Fonctions Membranaires, DBJC CEA et URA CNRS 2096 C E A Saclay, 91191 Gif Yvette cedex, France
    FEBS Lett 528:222-6. 2002
    The core light-harvesting complex (LH1) of Rhodospirillum rubrum is constituted of multiple heterodimeric subunits, each containing two transmembrane polypeptides, alpha and beta...
  100. doi Subtle spectral effects accompanying the assembly of bacteriochlorophylls into cyclic light harvesting complexes revealed by high-resolution fluorescence spectroscopy
    Margus Rätsep
    Institute of Physics, University of Tartu, Riia 142, 51014 Tartu, Estonia
    J Chem Phys 141:155102. 2014
    ..the bacteriochloropyll a molecules into B850 and B875 groups of cyclic bacterial light-harvesting complexes LH2 and LH1, respectively, results an almost total loss of the intra-molecular vibronic structure in the fluorescence spectrum, ..
  101. ncbi Characterization of a highly purified, fully active, crystallizable RC-LH1-PufX core complex from Rhodobacter sphaeroides
    E C Abresch
    Department of Physics, University California San Diego, 9500 Gilman Dr, La Jolla, CA 92093, USA
    Photosynth Res 86:61-70. 2005
    ..We describe the isolation of a highly purified, active, reaction center-light-harvesting 1-PufX complex (RC-LH1-PufX core complex) from a strain of the photosynthetic bacterium, Rhodobacter sphaeroides, which lacks the light-..

Research Grants21

  1. Collagen remodeling and tumor progression
    Valerie Marie Weaver; Fiscal Year: 2013
    ..The lysyl oxidase and lysyl hydroxylase family of enzymes are also expressed in the stroma where they cross-link collagen fibrils to enhance the ..
  2. Collagen Glycosylation, Maturation and Mineralization
    Mitsuo Yamauchi; Fiscal Year: 2013
    ..Most recently we have demonstrated that lysyl hydroxylase 3 functions as GGT and, possibly, glycosyltransferase 25 family member 1 (GLT25D1) as GT for bone type I ..
  3. SKIN REACTION TO LIGHT--NORMAL, PHOTOSENSITIVITY, CANCER
    MADHUKAR PATHAK; Fiscal Year: 1992
    ..determination of Type I and III collagen, cross-links and collagen, changes in 4-OH-prolyl-4'- hydroxylase, lysyl hydroxylase, desmosine, isodesmosine, and proteoglycans and evaluation of the role of certain free radical scavengers ..
  4. MOLECULAR AND BIOCHEMICAL STUDY OF COLLAGEN IN PROLAPSE
    Anthony Visco; Fiscal Year: 2002
    ..for the cross-linking process and the level of hydroxylation varies among tissues, Lysyl oxidase and lysyl hydroxylase are two enzymes involved in the early steps of the cross-linking process...
  5. COLLAGEN BIOSYNTHESIS IN HUMAN SKIN FIBROBLASTS
    Sheldon Pinnell; Fiscal Year: 1999
    ..mechanisms responsible for: (1) Inhibition of collagen synthesis by benzoic hydrazide; and (2) inhibition of lysyl hydroxylase expression by minoxidil...
  6. LUNG COLLAGEN CROSSLINKING--BIOSYNTHESIS AND MATURATION
    Jerold Last; Fiscal Year: 1992
    ..collagen" from "normal collagen" in the lung; (ii) that this difference is mediated by increased activity of lysyl hydroxylase in the (pre)fibrotic lung; and (iii) that once crosslinked, "fibrotic collagen" in the lung is not subject ..
  7. REGULATION OF COLLAGEN MATRIX MINERALIZATION
    Mitsuo Yamauchi; Fiscal Year: 2007
    ..To establish osteoblastic cell clones that synthesize low levels of lysyl hydroxylase-2 to switch the cross-linking pathway from mineralized to non-mineralized tissue phenotype, 1b...
  8. Phase 3A of a 3-phase 1.3-GHz LTS/HTS NMR magnet
    YUKIKAZU NA IWASA; Fiscal Year: 2013
    ..3 GHz LTS/HTS NMR magnet (LH1.3G)...
  9. CHARACTERIZATION OF MEMBRANE PROTEIN BY SSNMR
    Ann McDermott; Fiscal Year: 2001
    ..and side-chain chemical shifts of hydrated micro-crystalline U-13C, 15N BPTI and of the intrinsic membrane protein, LH1. Typical 13C peak linewidths are 0...
  10. Structural Studies of Photosynthetic Membrane Proteins
    MATTHEW CRADDOCK; Fiscal Year: 2007
    ..understanding of this efficiency is a detailed structural knowledge of the major components of the antenna system, LH1, LH2, and the Reaction Center (RC), which are all membrane proteins...
  11. MEMBRANE PROTEINS--STRUCTURE, DYNAMICS AND STABILITY
    CRAIG SCHENCK; Fiscal Year: 2002
    ..b>LH1 protein, a 200 kD hetero-oligomeric membrane protein from photoheterotrophic eubacteria, undergoes multiple ..
  12. CLIENTS WHO SEEK ON-LINE DRUG TREATMENT
    MAMIE WONG; Fiscal Year: 2003
    ..Without this "cultural competence," investigators may plod forth with increasingly complex studies that yield equally complex findings, with unknown generalizability to ..
  13. CHARACTERIZATION OF LYSYL HYDROXYLASE ISOFORMS IN SKIN
    Heather Yeowell; Fiscal Year: 2008
    ..The identity of the telopeptide lysyl hydroxylase therefore becomes important to establish...
  14. TWO CANDIDATE GENES LINKED TO LOW BONE DENSITY
    LORETTA SPOTILA; Fiscal Year: 2001
    ..Two candidate genes located within this interval are PLOD, the gene for lysyl hydroxylase, and TNFR2, the gene for tumor necrosis factor-alpha receptor 2...
  15. COLLAGEN CROSS-LINKING IN SKELETAL AGING AND DISEASE
    David R Eyre; Fiscal Year: 2010
    ..In parallel with basic tissue studies, collagen fragments in urine are being examined by similar methods as potential non-invasive biomarkers of a patient's bone and joint health. ..
  16. ACTIVATION OF TONGUE MUSCLES IN OBSTRUCTIVE SLEEP APNEA
    Dominique Durand; Fiscal Year: 2004
    ..It is anticipated that the experimental delineation and validation of these methods will lead to a design of a neuroprosthetic device for the treatment of OSA. ..
  17. Collagen type II/IX/XI heteropolymer assembly
    RUSSELL FERNANDES; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  18. WNT PROTEINS IN ADULT, AGED AND OSTEOARTHRITIC CARTILAGE
    Karen Yates; Fiscal Year: 2005
    ..This work will also provide a solid foundation for future research on Wnts in cartilage biology. ..
  19. Analysis & Control of Non-Synaptic Epileptiform Activity
    Dominique Durand; Fiscal Year: 2004
    ..The results of our studies should provide valuable insight into mechanisms underlying epileptogenesis as well as new tools for the control and suppression of epileptic seizures. ..
  20. TRANSCRIPTIONAL REGULATORS OF CHONDROINDUCTION BY DBP
    Karen Yates; Fiscal Year: 2004
    ..We will determine which DBP-regulated transcription factors are necessary and/or sufficient for postnatal chondrogenesis. This proposed work would have a high impact of design of strategies for repair and/or regeneration of cartilage. ..