LGR5

Summary

Gene Symbol: LGR5
Description: leucine rich repeat containing G protein-coupled receptor 5
Alias: FEX, GPR49, GPR67, GRP49, HG38, leucine-rich repeat-containing G-protein coupled receptor 5, G-protein coupled receptor 49, G-protein coupled receptor 67, G-protein coupled receptor HG38, orphan G protein-coupled receptor HG38
Species: human
Products:     LGR5

Top Publications

  1. Hsu S, Liang S, Hsueh A. Characterization of two LGR genes homologous to gonadotropin and thyrotropin receptors with extracellular leucine-rich repeats and a G protein-coupled, seven-transmembrane region. Mol Endocrinol. 1998;12:1830-45 pubmed
    ..We have identified two new leucine-rich repeat-containing, G protein-coupled receptors and named them as LGR4 and LGR5, respectively...
  2. McClanahan T, Koseoglu S, Smith K, Grein J, Gustafson E, Black S, et al. Identification of overexpression of orphan G protein-coupled receptor GPR49 in human colon and ovarian primary tumors. Cancer Biol Ther. 2006;5:419-26 pubmed
    ..This analysis revealed that GPR49, an orphan G Protein-Coupled Receptor (GPCR) is overexpressed in 66% (10/15) colon tumors compared with normal ..
  3. Becker L, Huang Q, Mashimo H. Immunostaining of Lgr5, an intestinal stem cell marker, in normal and premalignant human gastrointestinal tissue. ScientificWorldJournal. 2008;8:1168-76 pubmed publisher
    b>Lgr5 has recently been identified as a murine marker of intestinal stem cells. Its expression has not been well characterized in human gastrointestinal tissues, but has been reported in certain cancers...
  4. Takeda K, Kinoshita I, Shimizu Y, Matsuno Y, Shichinohe T, Dosaka Akita H. Expression of LGR5, an intestinal stem cell marker, during each stage of colorectal tumorigenesis. Anticancer Res. 2011;31:263-70 pubmed
    The Wnt target LGR5 has been recently identified as a murine intestinal stem cell marker. Its role during each stage of human colorectal tumorigenesis remains to be determined...
  5. Zeggini E, Scott L, Saxena R, Voight B, Marchini J, Hu T, et al. Meta-analysis of genome-wide association data and large-scale replication identifies additional susceptibility loci for type 2 diabetes. Nat Genet. 2008;40:638-45 pubmed publisher
    ..0 x 10(-14)), CDC123-CAMK1D (P = 1.2 x 10(-10)), TSPAN8-LGR5 (P = 1.1 x 10(-9)), THADA (P = 1.1 x 10(-9)), ADAMTS9 (P = 1.2 x 10(-8)) and NOTCH2 (P = 4.1 x 10(-8)) gene regions...
  6. McDonald T, Wang R, Bailey W, Xie G, Chen F, Caskey C, et al. Identification and cloning of an orphan G protein-coupled receptor of the glycoprotein hormone receptor subfamily. Biochem Biophys Res Commun. 1998;247:266-70 pubmed
    ..This gene, designated HG38, is predicted to encode a polypeptide of 907 amino acid residues with a putative signal peptide sequence at its ..
  7. de Lau W, Barker N, Low T, Koo B, Li V, Teunissen H, et al. Lgr5 homologues associate with Wnt receptors and mediate R-spondin signalling. Nature. 2011;476:293-7 pubmed publisher
    The adult stem cell marker Lgr5 and its relative Lgr4 are often co-expressed in Wnt-driven proliferative compartments...
  8. Kwon M, Park B, Kim H, Kim S. Leucine-rich repeat-containing G-protein coupled receptor 5/GPR49 activates G12/13-Rho GTPase pathway. Mol Cells. 2013;36:267-72 pubmed publisher
    Leucine-rich repeat-containing G-protein coupled receptor 5 (LGR5/GPR49) is highly expressed in adult stem cells of various tissues, such as intestine, hair follicles, and stomach...
  9. Ruffner H, Sprunger J, Charlat O, Leighton Davies J, Grosshans B, Salathe A, et al. R-Spondin potentiates Wnt/?-catenin signaling through orphan receptors LGR4 and LGR5. PLoS ONE. 2012;7:e40976 pubmed publisher
    ..The loss of LGR4 could be compensated by overexpression of LGR5, suggesting that LGR4 and LGR5 are functional homologs...

More Information

Publications81

  1. Simon E, Petke D, Böger C, Behrens H, Warneke V, Ebert M, et al. The spatial distribution of LGR5+ cells correlates with gastric cancer progression. PLoS ONE. 2012;7:e35486 pubmed publisher
    ..distribution and tumour biological significance of the Wnt target protein and cancer stem cell marker LGR5 in tumours of the human gastrointestinal tract...
  2. Barker N, Rookmaaker M, Kujala P, Ng A, Leushacke M, Snippert H, et al. Lgr5(+ve) stem/progenitor cells contribute to nephron formation during kidney development. Cell Rep. 2012;2:540-52 pubmed publisher
    ..Here, we document expression of the adult stem cell marker Lgr5 in the developing kidney and assess the stem/progenitor identity of Lgr5(+ve) cells via in vivo lineage tracing...
  3. Al Kharusi M, Smartt H, Greenhough A, Collard T, Emery E, Williams A, et al. LGR5 promotes survival in human colorectal adenoma cells and is upregulated by PGE2: implications for targeting adenoma stem cells with NSAIDs. Carcinogenesis. 2013;34:1150-7 pubmed publisher
    ..Given that the key intestinal cancer stem cell marker LGR5 (leucine-rich G-protein coupled receptor 5) is a Wnt target and PGE2 enhances Wnt signalling, the focus of this ..
  4. Wang D, Zhou J, Fan C, Jiao F, Liu B, Sun P, et al. Knockdown of LGR5 suppresses the proliferation of glioma cells in vitro and in vivo. Oncol Rep. 2014;31:41-9 pubmed publisher
    Leucine-rich repeat containing G protein-coupled receptor 5 (LGR5), one of the target genes of the Wnt signaling pathway, has recently been identified as a marker for brain cancer stem-like cells...
  5. Lango H, Palmer C, Morris A, Zeggini E, Hattersley A, McCarthy M, et al. Assessing the combined impact of 18 common genetic variants of modest effect sizes on type 2 diabetes risk. Diabetes. 2008;57:3129-35 pubmed publisher
    ..Further studies are needed to assess whether individuals with extreme numbers of risk alleles may benefit from genetic testing. ..
  6. Rot S, Taubert H, Bache M, Greither T, Wurl P, Eckert A, et al. A novel splice variant of the stem cell marker LGR5/GPR49 is correlated with the risk of tumor-related death in soft-tissue sarcoma patients. BMC Cancer. 2011;11:429 pubmed publisher
    The human leucine-rich, repeat-containing G protein-coupled receptor (LGR) 5, also called GPR49, is a marker of stem cells in adult intestinal epithelium, stomach and hair follicles...
  7. Kobayashi S, Yamada Okabe H, Suzuki M, Natori O, Kato A, Matsubara K, et al. LGR5-positive colon cancer stem cells interconvert with drug-resistant LGR5-negative cells and are capable of tumor reconstitution. Stem Cells. 2012;30:2631-44 pubmed publisher
    ..By generating specific antibodies against LGR5, we demonstrated that these cells expressed LGR5 and underwent self-renewal using symmetrical divisions...
  8. Yamamoto Y, Sakamoto M, Fujii G, Tsuiji H, Kenetaka K, Asaka M, et al. Overexpression of orphan G-protein-coupled receptor, Gpr49, in human hepatocellular carcinomas with beta-catenin mutations. Hepatology. 2003;37:528-33 pubmed
    ..Among these genes, Gpr49 was up-regulated in PLC/PRF/5 and HepG2...
  9. Carmon K, Gong X, Lin Q, Thomas A, Liu Q. R-spondins function as ligands of the orphan receptors LGR4 and LGR5 to regulate Wnt/beta-catenin signaling. Proc Natl Acad Sci U S A. 2011;108:11452-7 pubmed publisher
    ..b>LGR5, an orphan receptor of the G protein-coupled receptor (GPCR) superfamily, is specifically expressed in stem cells ..
  10. von Rahden B, Kircher S, Lazariotou M, Reiber C, Stuermer L, Otto C, et al. LgR5 expression and cancer stem cell hypothesis: clue to define the true origin of esophageal adenocarcinomas with and without Barrett's esophagus?. J Exp Clin Cancer Res. 2011;30:23 pubmed publisher
    ..Expression of a putative intestinal stem cell marker LgR5 was analyzed in esophageal cancer specimen (n = 70: 41 EAC with BE, 19 EAC without BE, and n = 10 esophageal ..
  11. Wu C, Xie Y, Gao F, Wang Y, Guo Y, Tian H, et al. Lgr5 expression as stem cell marker in human gastric gland and its relatedness with other putative cancer stem cell markers. Gene. 2013;525:18-25 pubmed publisher
    To explore Lgr5 as the possible stem cell marker in human gastric tissue, 259 normal gastric tissues and dissected gastric adenocarcinoma were analyzed by immunohistochemistry, immunofluorescence double staining and qRT-PCR...
  12. Fan X, Wu H, Yu H, Zhou Q, Zhang Y, Huang Q. Expression of Lgr5 in human colorectal carcinogenesis and its potential correlation with beta-catenin. Int J Colorectal Dis. 2010;25:583-90 pubmed publisher
    b>Lgr5 is a member of the G protein receptor super-family and was shown recently to be a stem cell marker for cells with intestinal differentiation...
  13. Hsu S, Kudo M, Chen T, Nakabayashi K, Bhalla A, van der Spek P, et al. The three subfamilies of leucine-rich repeat-containing G protein-coupled receptors (LGR): identification of LGR6 and LGR7 and the signaling mechanism for LGR7. Mol Endocrinol. 2000;14:1257-71 pubmed
    ..to two recently isolated mammalian LGRs (leucine-rich repeat-containing, G protein-coupled receptors), LGR4 and LGR5, we further identified two new paralogs, LGR6 and LGR7, for glycoprotein hormone receptors...
  14. Becker L, Huang Q, Mashimo H. Lgr5, an intestinal stem cell marker, is abnormally expressed in Barrett's esophagus and esophageal adenocarcinoma. Dis Esophagus. 2010;23:168-74 pubmed publisher
    b>Lgr5 (leucine-rich-repeat-containing G-protein-coupled receptor 5), a recently discovered intestinal stem cell marker, is expressed in premalignant lesions including Barrett's esophagus (BE) and cancers including colon cancer, ovarian ..
  15. Fukuma M, Tanese K, Effendi K, Yamazaki K, Masugi Y, Suda M, et al. Leucine-rich repeat-containing G protein-coupled receptor 5 regulates epithelial cell phenotype and survival of hepatocellular carcinoma cells. Exp Cell Res. 2013;319:113-21 pubmed publisher
    The leucine-rich repeat containing G protein-coupled receptor 5 (LGR5), also known as GPR49, is a seven-transmembrane receptor that is expressed in stem cells of the intestinal crypts and hair follicles of mice...
  16. Hsu H, Liu Y, Tseng K, Hsu C, Liang Y, Yang T, et al. Overexpression of Lgr5 correlates with resistance to 5-FU-based chemotherapy in colorectal cancer. Int J Colorectal Dis. 2013;28:1535-46 pubmed publisher
    The leucine-rich repeat-containing G protein-coupled receptor 5 (Lgr5) is an adult intestinal stem cell marker frequently detected in human colorectal cancers (CRCs)...
  17. Meigs J, Shrader P, Sullivan L, McAteer J, Fox C, Dupuis J, et al. Genotype score in addition to common risk factors for prediction of type 2 diabetes. N Engl J Med. 2008;359:2208-19 pubmed publisher
    ..A genotype score based on 18 risk alleles predicted new cases of diabetes in the community but provided only a slightly better prediction of risk than knowledge of common risk factors alone. ..
  18. Carmon K, Lin Q, Gong X, Thomas A, Liu Q. LGR5 interacts and cointernalizes with Wnt receptors to modulate Wnt/?-catenin signaling. Mol Cell Biol. 2012;32:2054-64 pubmed publisher
    b>LGR5, a seven-transmembrane domain receptor of the rhodopsin family, is a Wnt target gene and a bona fide marker of adult stem cells in the gastrointestinal tract and hair follicle bulge...
  19. Saigusa S, Inoue Y, Tanaka K, Toiyama Y, Matsushita K, Kawamura M, et al. Clinical significance of LGR5 and CD44 expression in locally advanced rectal cancer after preoperative chemoradiotherapy. Int J Oncol. 2012;41:1643-52 pubmed publisher
    b>LGR5, known as a target of Wnt signaling, has been reported as an intestinal stem cell marker. Recent reports showed that LGR5 was associated with carcinogenesis and tumor invasion in colorectal cancer...
  20. Lyssenko V, Jonsson A, Almgren P, Pulizzi N, Isomaa B, Tuomi T, et al. Clinical risk factors, DNA variants, and the development of type 2 diabetes. N Engl J Med. 2008;359:2220-32 pubmed publisher
    ..The value of genetic factors increased with an increasing duration of follow-up. ..
  21. Takahashi H, Ishii H, Nishida N, Takemasa I, Mizushima T, Ikeda M, et al. Significance of Lgr5(+ve) cancer stem cells in the colon and rectum. Ann Surg Oncol. 2011;18:1166-74 pubmed publisher
    Although recent studies show that leucine-rich repeat-containing G-protein-coupled receptor 5 (Lgr5)(+ve) cells targeted by Wnt drive self-renewal in the skin and gastrointestinal organs, the clinicopathological significance of Lgr5(+ve) ..
  22. Tanese K, Fukuma M, Yamada T, Mori T, Yoshikawa T, Watanabe W, et al. G-protein-coupled receptor GPR49 is up-regulated in basal cell carcinoma and promotes cell proliferation and tumor formation. Am J Pathol. 2008;173:835-43 pubmed publisher
    ..formation in BCC, we screened a DNA microarray database of human BCC cases; the orphan G-protein-coupled receptor GPR49 was found to be up-regulated in all cases...
  23. Yamanoi K, Fukuma M, Uchida H, Kushima R, Yamazaki K, Katai H, et al. Overexpression of leucine-rich repeat-containing G protein-coupled receptor 5 in gastric cancer. Pathol Int. 2013;63:13-9 pubmed publisher
    ..The overexpression of leucine-rich repeat-containing G protein-coupled receptor 5 (LGR5) mRNA in colorectal cancer and its correlation with clinicopathological factors were recently reported by us...
  24. Barker N, van Es J, Kuipers J, Kujala P, van den Born M, Cozijnsen M, et al. Identification of stem cells in small intestine and colon by marker gene Lgr5. Nature. 2007;449:1003-7 pubmed
    ..Lgr5 (leucine-rich-repeat-containing G-protein-coupled receptor 5, also known as Gpr49) was selected from a panel of intestinal Wnt target genes for its restricted crypt expression...
  25. Haegebarth A, Clevers H. Wnt signaling, lgr5, and stem cells in the intestine and skin. Am J Pathol. 2009;174:715-21 pubmed publisher
    ..The Wnt target gene Lgr5 has been recently identified as a novel stem cell marker of the intestinal epithelium and the hair follicle...
  26. Uchida H, Yamazaki K, Fukuma M, Yamada T, Hayashida T, Hasegawa H, et al. Overexpression of leucine-rich repeat-containing G protein-coupled receptor 5 in colorectal cancer. Cancer Sci. 2010;101:1731-7 pubmed publisher
    Leucine-rich repeat-containing G protein-coupled receptor 5 (LGR5) is a 7-transmembrane receptor reportedly expressed in stem cells of the intestinal crypts and hair follicles of mice...
  27. Nakata S, Campos B, Bageritz J, Bermejo J, Becker N, Engel F, et al. LGR5 is a marker of poor prognosis in glioblastoma and is required for survival of brain cancer stem-like cells. Brain Pathol. 2013;23:60-72 pubmed publisher
    ..b>LGR5, whose expression is positively regulated by the Wnt signaling pathway, is a stem cell marker in intestinal mucosa ..
  28. Walker F, Zhang H, Odorizzi A, Burgess A. LGR5 is a negative regulator of tumourigenicity, antagonizes Wnt signalling and regulates cell adhesion in colorectal cancer cell lines. PLoS ONE. 2011;6:e22733 pubmed publisher
    b>LGR5 (Leucine-rich repeat-containing G-protein coupled receptor 5) is the most established marker for intestinal stem cells...
  29. Valladares Ayerbes M, Blanco Calvo M, Reboredo M, Lorenzo Patiño M, Iglesias Díaz P, Haz M, et al. Evaluation of the adenocarcinoma-associated gene AGR2 and the intestinal stem cell marker LGR5 as biomarkers in colorectal cancer. Int J Mol Sci. 2012;13:4367-87 pubmed publisher
    ..performances of anterior gradient homolog-2 (AGR2) and Leucine-rich repeat-containing-G-protein-coupled receptor 5 (LGR5) in peripheral blood (PB) as mRNA biomarkers in colorectal cancer (CRC) and to explore their prognostic ..
  30. Kleist B, Xu L, Li G, Kersten C. Expression of the adult intestinal stem cell marker Lgr5 in the metastatic cascade of colorectal cancer. Int J Clin Exp Pathol. 2011;4:327-35 pubmed
    ..cancer stem cells are difficult to study due to the lack of reliable markers, but expression of the Wnt target gene Lgr5 is promising to define at least stem cell like cells in intestinal and colorectal cancer...
  31. Saigusa S, Inoue Y, Tanaka K, Toiyama Y, Kawamura M, Okugawa Y, et al. Significant correlation between LKB1 and LGR5 gene expression and the association with poor recurrence-free survival in rectal cancer after preoperative chemoradiotherapy. J Cancer Res Clin Oncol. 2013;139:131-8 pubmed publisher
    The aim of the present study was to investigate whether the gene expression levels of LKB1 and LGR5 correlated with clinical outcome in patients with locally advanced rectal cancer treated with preoperative chemoradiotherapy (CRT)...
  32. Kemper K, Prasetyanti P, de Lau W, Rodermond H, Clevers H, Medema J. Monoclonal antibodies against Lgr5 identify human colorectal cancer stem cells. Stem Cells. 2012;30:2378-86 pubmed publisher
    ..Here, we analyzed whether the Wnt target Lgr5, which has earlier been identified as a marker for murine intestinal stem cells, could potentially serve as a ..
  33. Uehara T, Ma D, Yao Y, Lynch J, Morales K, Ziober A, et al. H. pylori infection is associated with DNA damage of Lgr5-positive epithelial stem cells in the stomach of patients with gastric cancer. Dig Dis Sci. 2013;58:140-9 pubmed publisher
    ..To characterize the expression of Lgr5, a marker of epithelial stem cells in human gastric mucosa, to determine whether Hp infection affects Lgr5-positive ..
  34. Hou Q, Ye L, Liu H, Huang L, Yang Q, Turner J, et al. Lactobacillus accelerates ISCs regeneration to protect the integrity of intestinal mucosa through activation of STAT3 signaling pathway induced by LPLs secretion of IL-22. Cell Death Differ. 2018;25:1657-1670 pubmed publisher
    ..The repair process stimulated by L. reuteri D8 was also accompanied with increased numbers of Lgr5+ and lysozyme+ cells both in intestinal organoids and mice intestine...
  35. Wei L, Leibowitz B, Wang X, Epperly M, Greenberger J, Zhang L, et al. Inhibition of CDK4/6 protects against radiation-induced intestinal injury in mice. J Clin Invest. 2016;126:4076-4087 pubmed publisher
    ..PD treatment also enhanced LGR5+ stem cell survival and regeneration after radiation...
  36. Lehoczky J, Tabin C. Lgr6 marks nail stem cells and is required for digit tip regeneration. Proc Natl Acad Sci U S A. 2015;112:13249-54 pubmed publisher
    ..Here, we report on Lgr5 and Lgr6 (leucine-rich repeat-containing G protein-coupled receptor 5 and 6), two important agonists of the Wnt ..
  37. Miao J, Jiang Y, Wang D, Zhou J, Fan C, Jiao F, et al. Trichosanthin suppresses the proliferation of glioma cells by inhibiting LGR5 expression and the Wnt/β-catenin signaling pathway. Oncol Rep. 2015;34:2845-52 pubmed publisher
    ..to assess the expression of leucine‑rich repeat‑containing G protein‑coupled receptor 5 (LGR5) and key proteins in the Wnt/β‑catenin signaling pathway...
  38. Dame M, Attili D, McClintock S, Dedhia P, Ouillette P, Hardt O, et al. Identification, isolation and characterization of human LGR5-positive colon adenoma cells. Development. 2018;145: pubmed publisher
    The intestine is maintained by stem cells located at the base of crypts and distinguished by the expression of LGR5. Genetically engineered mouse models have provided a wealth of information about intestinal stem cells, whereas less is ..
  39. Lanfredini S, Olivero C, Borgogna C, Calati F, Powell K, Davies K, et al. HPV8 Field Cancerization in a Transgenic Mouse Model Is due to Lrig1+ Keratinocyte Stem Cell Expansion. J Invest Dermatol. 2017;137:2208-2216 pubmed publisher
    ..55%, P < 0.01, n = 7), and not in the CD34+, LGR5+, and LGR6+ keratinocyte stem cell populations. This was associated with a 2...
  40. Xi H, Zhang K, Li J, Cui J, Gao Y, Wei B, et al. RNAi-mediated inhibition of Lgr5 leads to decreased angiogenesis in gastric cancer. Oncotarget. 2017;8:31581-31591 pubmed publisher
    Leucine-rich repeat-containing G protein-coupled receptor 5 (Lgr5) is a novel gastric cancer marker. However, it is unclear whether it can play roles in tumor angiogenesis...
  41. Carmon K, Gong X, Yi J, Wu L, Thomas A, Moore C, et al. LGR5 receptor promotes cell-cell adhesion in stem cells and colon cancer cells via the IQGAP1-Rac1 pathway. J Biol Chem. 2017;292:14989-15001 pubmed publisher
    Leucine-rich repeat-containing G protein-coupled receptor 5 (LGR5) is a bona fide marker of adult stem cells in several epithelial tissues, most notably in the intestinal crypts, and is highly up-regulated in many colorectal, ..
  42. Zhang Y, Li P, Zhang F, Sun S, Cao Y. Lgr5 regulates the regeneration of lesioned nasal respiratory epithelium. Biochem Biophys Res Commun. 2016;481:195-200 pubmed publisher
    ..In this study, we found that adult stem cell marker leucine-rich repeat-containing G-protein coupled receptor 5 (Lgr5) is expressed in the mice nasal respiratory epithelium...
  43. Funahashi S, Suzuki Y, Nakano K, Kawai S, Suzuki M. Generation and characterization of monoclonal antibodies against human LGR6. J Biochem. 2017;161:361-368 pubmed publisher
    ..Hybridomas were screened by flow cytometry using LGR6-transfected cells. Because the molecules of LGR4, LGR5, and LGR6 are 50% homologous at the amino acid level, specificity of the mAbs was confirmed by transfectants ..
  44. Sanz Navarro M, Seidel K, Sun Z, Bertonnier Brouty L, Amendt B, Klein O, et al. Plasticity within the niche ensures the maintenance of a Sox2+ stem cell population in the mouse incisor. Development. 2018;145: pubmed publisher
    ..Furthermore, we show that Lgr5-expressing cells are a subpopulation of dental Sox2+ cells that also arise from Sox2<..
  45. Zebisch M, Xu Y, Krastev C, MacDonald B, Chen M, Gilbert R, et al. Structural and molecular basis of ZNRF3/RNF43 transmembrane ubiquitin ligase inhibition by the Wnt agonist R-spondin. Nat Commun. 2013;4:2787 pubmed publisher
  46. Hou M, Chen P, Chu P. LGR5 overexpression confers poor relapse-free survival in breast cancer patients. BMC Cancer. 2018;18:219 pubmed publisher
    ..Leucine-rich repeat-containing G protein-coupled receptor 5 (LGR5) has been identified as a CSC-associated Wnt-regulated target gene, but its clinical significance in the context of ..
  47. Peñarando J, Lopez Sanchez L, Mena R, Guil Luna S, Conde F, Hernandez V, et al. A role for endothelial nitric oxide synthase in intestinal stem cell proliferation and mesenchymal colorectal cancer. BMC Biol. 2018;16:3 pubmed publisher
    ..the scavenger carboxy-PTIO (c-PTIO) decreased the proliferation and the expression of stem-cell markers, such as Lgr5, Troy, Vav3, and Slc14a1, in these intestinal organoids...
  48. Di Claudio F, Muglia C, Smaldini P, Orsini Delgado M, Trejo F, Grigera J, et al. Use of a Collagen Membrane to Enhance the Survival of Primary Intestinal Epithelial Cells. J Cell Physiol. 2017;232:2489-2496 pubmed publisher
    ..Apoptosis was dramatically reduced and cultured enterocytes expressed cytokeratin and LGR5 (low frequency)...
  49. Schilderink R, Verseijden C, Seppen J, Muncan V, van den Brink G, Lambers T, et al. The SCFA butyrate stimulates the epithelial production of retinoic acid via inhibition of epithelial HDAC. Am J Physiol Gastrointest Liver Physiol. 2016;310:G1138-46 pubmed publisher
    ..maturation markers (Mucin-2 and villin) but did not consistently affect stemness markers or other Wnt target genes (lgr5, olfm4, ascl2, cdkn1)...
  50. Tsai Y, VanDussen K, Sawey E, Wade A, Kasper C, Rakshit S, et al. ADAM10 regulates Notch function in intestinal stem cells of mice. Gastroenterology. 2014;147:822-834.e13 pubmed publisher
    ..Vil-Cre;Adam10(f/f) mice) and conditional (Vil-CreER;Adam10(f/f) and Leucine-rich repeat-containing GPCR5 [Lgr5]-CreER;Adam10(f/f) mice) deletion of ADAM10...
  51. Scott A, Rouch J, Jabaji Z, Khalil H, Solorzano S, Lewis M, et al. Long-term renewable human intestinal epithelial stem cells as monolayers: A potential for clinical use. J Pediatr Surg. 2016;51:995-1000 pubmed publisher
    ..Less differentiated monolayers expressed high levels of gene marker LGR5, while more differentiated monolayers had higher expressions of CDX2, MUC2, LYZ, DEF5, and CHGA...
  52. Szkandera J, Herzog S, Pichler M, Stiegelbauer V, Stotz M, Schaberl Moser R, et al. LGR5 rs17109924 is a predictive genetic biomarker for time to recurrence in patients with colon cancer treated with 5-fluorouracil-based adjuvant chemotherapy. Pharmacogenomics J. 2015;15:391-6 pubmed publisher
    We recently found variants in cancer stem cell genes (CD44, ALCAM and LGR5) significantly associated with increased time to recurrence (TTR) in patients with stage III and high-risk stage II colon cancer treated with 5-fluorouracil (5-FU)-..
  53. Simonis Bik A, Nijpels G, van Haeften T, Houwing Duistermaat J, Boomsma D, Reiling E, et al. Gene variants in the novel type 2 diabetes loci CDC123/CAMK1D, THADA, ADAMTS9, BCL11A, and MTNR1B affect different aspects of pancreatic beta-cell function. Diabetes. 2010;59:293-301 pubmed publisher
    ..All subjects were genotyped for gene variants in JAZF1, CDC123/CAMK1D, TSPAN8/LGR5, THADA, ADAMTS9, NOTCH2/ADAMS30, DCD, VEGFA, BCL11A, HNF1B, WFS1, and MTNR1B...
  54. Wang Y, Jiang C, Fan L. Correlation of Musashi-1, Lgr5, and pEGFR expressions in human small intestinal adenocarcinomas. Tumour Biol. 2015;36:6075-82 pubmed publisher
    Recent studies have revealed that Musashi-1 and Lgr5 (leucine-rich-repeat-containing G-protein-coupled receptor 5) were putative stem cell genes...
  55. Katoh M. Canonical and non-canonical WNT signaling in cancer stem cells and their niches: Cellular heterogeneity, omics reprogramming, targeted therapy and tumor plasticity (Review). Int J Oncol. 2017;51:1357-1369 pubmed publisher
    ..signaling cascades and regulate expression of functional CSC markers, such as CD44, CD133 (PROM1), EPCAM and LGR5 (GPR49)...
  56. Amsterdam A, Raanan C, Schreiber L, Freyhan O, Schechtman L, Givol D. Localization of the stem cell markers LGR5 and Nanog in the normal and the cancerous human ovary and their inter-relationship. Acta Histochem. 2013;115:330-8 pubmed publisher
    b>LGR5 and Nanog were recently characterized as stem cell markers in various embryonic, adult and cancer stem cells...
  57. Kumar K, Burgess A, Gulbis J. Structure and function of LGR5: an enigmatic G-protein coupled receptor marking stem cells. Protein Sci. 2014;23:551-65 pubmed publisher
    ..One member of this class is the leucine-rich repeat-containing G-protein-coupled receptor 5 (LGR5), a stem cell marker in intestinal crypts, and mammary glands...
  58. Lin Y, Fang Z, Liu H, Wang L, Cheng Z, Tang N, et al. HGF/R-spondin1 rescues liver dysfunction through the induction of Lgr5+ liver stem cells. Nat Commun. 2017;8:1175 pubmed publisher
    ..Carbon tetrachloride treatment promotes both fibrosis and Lgr5+ liver stem cell proliferation, whereas Lgr5 knockdown worsens fibrosis...
  59. Lee J, Tammela T, Hofree M, Choi J, Marjanovic N, Han S, et al. Anatomically and Functionally Distinct Lung Mesenchymal Populations Marked by Lgr5 and Lgr6. Cell. 2017;170:1149-1163.e12 pubmed publisher
    ..We used genetic lineage tracing, single-cell RNA sequencing, and organoid culture approaches to show that Lgr5 and Lgr6, well-known markers of stem cells in epithelial tissues, are markers of mesenchymal cells in the adult ..
  60. Karunanithi S, Levi L, DeVecchio J, Karagkounis G, Reizes O, Lathia J, et al. RBP4-STRA6 Pathway Drives Cancer Stem Cell Maintenance and Mediates High-Fat Diet-Induced Colon Carcinogenesis. Stem Cell Reports. 2017;9:438-450 pubmed publisher
    ..Furthermore, we show that high-fat diet (HFD) increases LGR5 expression and promotes tumor growth in a xenograft model independent of obesity...
  61. Han Y, Zhi Q, Xue X, Yuan B, Zhao H, Kuang Y, et al. Lgr5 Contributes to Intestinal Metaplasia During Gastric Carcinogenesis: A Meta analysis. Anticancer Agents Med Chem. 2016;16:1079-84 pubmed
    b>Lgr5, which is a somatic stem cell biomarker, plays an important role during the carcinogenesis and tumor progression. But in gastric cancer, the functions of Lgr5 still remain controversial...
  62. Basak O, Beumer J, Wiebrands K, Seno H, van Oudenaarden A, Clevers H. Induced Quiescence of Lgr5+ Stem Cells in Intestinal Organoids Enables Differentiation of Hormone-Producing Enteroendocrine Cells. Cell Stem Cell. 2017;20:177-190.e4 pubmed publisher
    b>Lgr5+ adult intestinal stem cells are highly proliferative throughout life. Single Lgr5+ stem cells can be cultured into three-dimensional organoids containing all intestinal epithelial cell types at near-normal ratios...
  63. Sigal M, Logan C, Kapalczynska M, Mollenkopf H, Berger H, Wiedenmann B, et al. Stromal R-spondin orchestrates gastric epithelial stem cells and gland homeostasis. Nature. 2017;548:451-455 pubmed publisher
    ..Axin2 is expressed by Lgr5+ cells, as well as adjacent, highly proliferative Lgr5- cells that are able to repopulate ..
  64. Zhao H, Wen J, Dong X, He R, Gao C, Zhang W, et al. Identification of AQP3 and CD24 as biomarkers for carcinogenesis of gastric intestinal metaplasia. Oncotarget. 2017;8:63382-63391 pubmed publisher
    ..CD24, LGR5 and Ki67 are expressed in GIM; we previously demonstrated that aquaporin 3 (AQP3) is expressed in goblet cells and ..
  65. Tong Z, Martyn K, Yang A, Yin X, Mead B, Joshi N, et al. Towards a defined ECM and small molecule based monolayer culture system for the expansion of mouse and human intestinal stem cells. Biomaterials. 2018;154:60-73 pubmed publisher
    ..culture system that supports the growth of mouse and human intestinal epithelial cells (IECs) enriched for an Lgr5+ population comparable or higher to the levels found in a standard Matrigel-based organoid culture...
  66. Morgan R, Mortensson E, Legge D, Gupta B, Collard T, Greenhough A, et al. LGR5 expression is regulated by EGF in early colorectal adenomas and governs EGFR inhibitor sensitivity. Br J Cancer. 2017;: pubmed publisher
    b>LGR5 serves as a co-receptor for Wnt/?-catenin signalling and marks normal intestinal stem cells; however, its role in colorectal cancer (CRC) remains controversial...
  67. Brzeszczynska J, Ramaesh K, Dhillon B, Ross J. Molecular profile of organ culture-stored corneal epithelium: LGR5 is a potential new phenotypic marker of residual human corneal limbal epithelial stem cells. Int J Mol Med. 2012;29:871-6 pubmed publisher
    ..Furthermore, we examined global markers of adult stem cells including p63? and ABCG2 but also LGR5 as a novel stem cell marker...
  68. Satoh J, Obayashi S, Tabunoki H, Wakana T, Kim S. Stable expression of neurogenin 1 induces LGR5, a novel stem cell marker, in an immortalized human neural stem cell line HB1.F3. Cell Mol Neurobiol. 2010;30:415-26 pubmed publisher
    ..Notably, leucine-rich repeat-containing G protein-coupled receptor 5 (LGR5), a novel stem cell marker, showed an 167-fold increase in F3-Ngn1, although transient overexpression of Ngn1 did ..
  69. Yu W, Ma Y, Shankar S, Srivastava R. SATB2/?-catenin/TCF-LEF pathway induces cellular transformation by generating cancer stem cells in colorectal cancer. Sci Rep. 2017;7:10939 pubmed publisher
    ..and these transformed cells gained the characteristics of CSCs by expressing stem cell markers (CD44, CD133, LGR5 and DCLK1) and transcription factors (c-Myc, Nanog and Sox2)...
  70. Vieira G, Chockalingam S, Melegh Z, Greenhough A, Malik S, Szemes M, et al. LGR5 regulates pro-survival MEK/ERK and proliferative Wnt/β-catenin signalling in neuroblastoma. Oncotarget. 2015;6:40053-67 pubmed publisher
    b>LGR5 is a marker of normal and cancer stem cells in various tissues where it functions as a receptor for R-spondins and increases canonical Wnt signalling amplitude...
  71. Fernandez Vallone V, Leprovots M, Strollo S, Vasile G, Lefort A, Libert F, et al. Trop2 marks transient gastric fetal epithelium and adult regenerating cells after epithelial damage. Development. 2016;143:1452-63 pubmed publisher
    ..Trop2(+) cells generating gastric spheroids differed from adult glandular Lgr5(+) stem cells, but appeared highly related to fetal intestinal spheroids...
  72. Stanisavljević L, Myklebust M, Leh S, Dahl O. LGR5 and CD133 as prognostic and predictive markers for fluoropyrimidine-based adjuvant chemotherapy in colorectal cancer. Acta Oncol. 2016;55:1425-1433 pubmed
    Expression of leucine-rich-repeat-containing G-protein-coupled receptor 5 (LGR5) gene is associated with a metastatic phenotype and poor prognosis in colorectal cancer (CRC)...