Lck

Summary

Gene Symbol: Lck
Description: lymphocyte-specific protein tyrosine kinase
Alias: LSK, YT16, p56lck, pp58lck, T-lymphocyte specific protein tyrosine kinase p56lck, leukocyte C-terminal Src kinase, lymphocyte cell-specific protein-tyrosine kinase, p56(LSTRA) protein-tyrosine kinase, proto-oncogene tyrosine-protein kinase LCK, t cell-specific protein-tyrosine kinase, tyrosine-protein kinase Lck
Species: human

Top Publications

  1. ncbi Function of the Src-family kinases, Lck and Fyn, in T-cell development and activation
    Emil H Palacios
    Rosalind Russell Medical Research Center for Arthritis, Department of Medicine and The Howard Hughes Medical Institute, University of California, San Francisco 94143 0795, USA
    Oncogene 23:7990-8000. 2004
  2. ncbi SHP2-interacting transmembrane adaptor protein (SIT), a novel disulfide-linked dimer regulating human T cell activation
    A Marie-Cardine
    Immunomodulation Laboratory of the Institute for Immunology, University of Heidelberg, 69120 Heidelberg, Germany
    J Exp Med 189:1181-94. 1999
  3. ncbi Specific dephosphorylation of the Lck tyrosine protein kinase at Tyr-394 by the SHP-1 protein-tyrosine phosphatase
    G G Chiang
    Molecular and Cell Biology Laboratory, The Salk Institute for Biological Studies, La Jolla, California 92037, USA
    J Biol Chem 276:23173-8. 2001
  4. ncbi A weak Lck tail bite is necessary for Lck function in T cell antigen receptor signaling
    Konstantina Nika
    Program on Inflammatory Disease Research, Infectious and Inflammatory Disease Center, The Burnham Institute for Medical Research, La Jolla, California 92037, USA
    J Biol Chem 282:36000-9. 2007
  5. ncbi Constitutively active Lck kinase in T cells drives antigen receptor signal transduction
    Konstantina Nika
    T Cell Signaling Laboratory, Sir William Dunn School of Pathology, University of Oxford, Oxford OX1 3RE, UK
    Immunity 32:766-77. 2010
  6. ncbi Human homologue of the Drosophila discs large tumor suppressor binds to p56lck tyrosine kinase and Shaker type Kv1.3 potassium channel in T lymphocytes
    T Hanada
    Laboratory of Tumor Cell Biology, St Elizabeth s Medical Center, Tufts University School of Medicine, Boston, Massachusetts 02135, USA
    J Biol Chem 272:26899-904. 1997
  7. ncbi Tyrosine phosphorylation sites at amino acids 239 and 240 of Shc are involved in epidermal growth factor-induced mitogenic signaling that is distinct from Ras/mitogen-activated protein kinase activation
    N Gotoh
    Department of Genetics, Institute of Medical Science, University of Tokyo, Japan
    Mol Cell Biol 17:1824-31. 1997
  8. ncbi Lck phosphorylates the activation loop tyrosine of the Itk kinase domain and activates Itk kinase activity
    S D Heyeck
    Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts 02138, USA
    J Biol Chem 272:25401-8. 1997
  9. ncbi Phosphatidylinositol (PI) 3-kinase and PI 4-kinase binding to the CD4-p56lck complex: the p56lck SH3 domain binds to PI 3-kinase but not PI 4-kinase
    K V Prasad
    Division of Tumor Immunology, Dana Farber Cancer Institute, Boston, Massachusetts 02115
    Mol Cell Biol 13:7708-17. 1993
  10. ncbi Regulation of Fyn through translocation of activated Lck into lipid rafts
    Dominik Filipp
    Sunnybrook and Women s College Health Sciences Centre, and Departmentof Immunology, University of Toronto, Ontario M4N 3M5, Canada
    J Exp Med 197:1221-7. 2003

Research Grants

  1. Genetic and Immunological Impact of the HRES-1/Rab4 Locus in SLE
    Andras Perl; Fiscal Year: 2010
  2. p56lck Inhibitors as Potential Immunosuppressive Drugs.
    ALEXANDER MACKERELL; Fiscal Year: 2004
  3. TYROSINE PROTEIN KINASES AND LYMPHOCYTE ACTIVATION
    ROBERT GEAHLEN; Fiscal Year: 1993
  4. TYROSINE PROTEIN KINASES AND LYMPHOCYTE ACTIVATION
    ROBERT GEAHLEN; Fiscal Year: 1991
  5. Role of Src Kinases in T Cell Receptor Signaling
    David Straus; Fiscal Year: 2004
  6. Negative regulation of TCR-associated PTKs
    Tomas Mustelin; Fiscal Year: 2006
  7. Negative regulation of TCR-associated PTKs
    ROBERT RICKERT; Fiscal Year: 2007
  8. Src Family Kinases as Molecular Targets for HIV/Nef
    THOMAS SMITHGALL; Fiscal Year: 2007
  9. Src Family Kinases as Molecular Targets for HIV/Nef
    THOMAS EDWARD SMITHGALL; Fiscal Year: 2010
  10. Phosphoproteomic Analysis of T Cell Activation Pathways
    ARTHUR ROBERT SALOMON; Fiscal Year: 2010

Detail Information

Publications197 found, 100 shown here

  1. ncbi Function of the Src-family kinases, Lck and Fyn, in T-cell development and activation
    Emil H Palacios
    Rosalind Russell Medical Research Center for Arthritis, Department of Medicine and The Howard Hughes Medical Institute, University of California, San Francisco 94143 0795, USA
    Oncogene 23:7990-8000. 2004
    The function of the Src-family kinases (SFKs) Lck and Fyn in T cells has been intensively studied over the past 15 years...
  2. ncbi SHP2-interacting transmembrane adaptor protein (SIT), a novel disulfide-linked dimer regulating human T cell activation
    A Marie-Cardine
    Immunomodulation Laboratory of the Institute for Immunology, University of Heidelberg, 69120 Heidelberg, Germany
    J Exp Med 189:1181-94. 1999
    ..However, binding of SHP2 to SIT is not required for inhibition of NF-AT induction, suggesting that SIT not only regulates NF-AT activity but also controls NF-AT unrelated pathways of T cell activation involving SHP2...
  3. ncbi Specific dephosphorylation of the Lck tyrosine protein kinase at Tyr-394 by the SHP-1 protein-tyrosine phosphatase
    G G Chiang
    Molecular and Cell Biology Laboratory, The Salk Institute for Biological Studies, La Jolla, California 92037, USA
    J Biol Chem 276:23173-8. 2001
    ..In this study, we demonstrate that SHP-1 dephosphorylates the lymphoid-specific Src family kinase Lck at Tyr-394 when both are transiently co-expressed in nonlymphoid cells...
  4. ncbi A weak Lck tail bite is necessary for Lck function in T cell antigen receptor signaling
    Konstantina Nika
    Program on Inflammatory Disease Research, Infectious and Inflammatory Disease Center, The Burnham Institute for Medical Research, La Jolla, California 92037, USA
    J Biol Chem 282:36000-9. 2007
    ..understand the relative importance of these two opposite functions of the SH2 domain of the Src family kinase Lck in TCR signaling, we created three mutants of Lck in which the intramolecular binding of the C terminus to the SH2 ..
  5. ncbi Constitutively active Lck kinase in T cells drives antigen receptor signal transduction
    Konstantina Nika
    T Cell Signaling Laboratory, Sir William Dunn School of Pathology, University of Oxford, Oxford OX1 3RE, UK
    Immunity 32:766-77. 2010
    ..TCR) and coreceptor ligation is thought to initiate signal transduction by inducing activation of the kinase Lck. Here we showed that catalytically active Lck was present in unstimulated naive T cells and thymocytes and was ..
  6. ncbi Human homologue of the Drosophila discs large tumor suppressor binds to p56lck tyrosine kinase and Shaker type Kv1.3 potassium channel in T lymphocytes
    T Hanada
    Laboratory of Tumor Cell Biology, St Elizabeth s Medical Center, Tufts University School of Medicine, Boston, Massachusetts 02135, USA
    J Biol Chem 272:26899-904. 1997
    ..Immunoblotting experiments revealed that the immunoprecipitates of hDlg contain p56lck, a member of the Src family of tyrosine kinases...
  7. ncbi Tyrosine phosphorylation sites at amino acids 239 and 240 of Shc are involved in epidermal growth factor-induced mitogenic signaling that is distinct from Ras/mitogen-activated protein kinase activation
    N Gotoh
    Department of Genetics, Institute of Medical Science, University of Tokyo, Japan
    Mol Cell Biol 17:1824-31. 1997
    ..These results suggest that Shc activates two distinct signaling pathways, Y317 to Ras/MAPK and Y239 and Y240 to another pathway including Myc, and that both are involved in EGF-induced mitogenic signaling...
  8. ncbi Lck phosphorylates the activation loop tyrosine of the Itk kinase domain and activates Itk kinase activity
    S D Heyeck
    Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts 02138, USA
    J Biol Chem 272:25401-8. 1997
    ..tyrosine-phosphorylated after TCR cross-linking and that this phosphorylation depends on the presence of functional Lck. To determine if this Lck dependence results from direct phosphorylation of Itk by Lck, we generated recombinant ..
  9. ncbi Phosphatidylinositol (PI) 3-kinase and PI 4-kinase binding to the CD4-p56lck complex: the p56lck SH3 domain binds to PI 3-kinase but not PI 4-kinase
    K V Prasad
    Division of Tumor Immunology, Dana Farber Cancer Institute, Boston, Massachusetts 02115
    Mol Cell Biol 13:7708-17. 1993
    ..It is coupled to the protein-tyrosine kinase p56lck, an interaction necessary for an optimal response of certain T cells to antigen...
  10. ncbi Regulation of Fyn through translocation of activated Lck into lipid rafts
    Dominik Filipp
    Sunnybrook and Women s College Health Sciences Centre, and Departmentof Immunology, University of Toronto, Ontario M4N 3M5, Canada
    J Exp Med 197:1221-7. 2003
    Whether or how the activation of Lck and Fyn during T cell receptor (TCR) signaling is coordinated, and their delivery of function integrated, is unknown...
  11. ncbi Structural basis for activation of human lymphocyte kinase Lck upon tyrosine phosphorylation
    H Yamaguchi
    Department of Biochemistry and Molecular Biophysics, Columbia University, New York 10032, USA
    Nature 384:484-9. 1996
    Regulation through phosphorylation is a characteristic of signalling pathways and the lymphocyte kinase Lck (p56lck) both performs phosphorylation and is affected by it...
  12. ncbi Identification of preferred protein interactions by phage-display of the human Src homology-3 proteome
    Satu Kärkkäinen
    Institute of Medical Technology, University of Tampere and Tampere University Hospital, Biokatu 8, Tampere 33014, Finland
    EMBO Rep 7:186-91. 2006
    ....
  13. ncbi The SH3 domain of p56lck binds to proline-rich sequences in the cytoplasmic domain of CD2
    G M Bell
    Department of Medicine, Veterans Affairs Medical Center, San Francisco, California, USA
    J Exp Med 183:169-78. 1996
    ..An Src-like protein tyrosine kinase, p56lck, coprecipitates with CD2, and perturbation of CD2 by monoclonal antibodies results in an increase in the activity ..
  14. ncbi Cutting edge: the CD45 tyrosine phosphatase is an inhibitor of Lck activity in thymocytes
    U D'Oro
    Laboratory of Immune Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    J Immunol 162:1879-83. 1999
    A widely accepted model for regulation of the Lck tyrosine kinase is that it is activated by CD45-mediated dephosphorylation of its COOH-terminal negative regulatory tyrosine (Tyr505)...
  15. ncbi Genetically encoded Förster resonance energy transfer sensors for the conformation of the Src family kinase Lck
    Wolfgang Paster
    Department of Molecular Immunology, Center for Physiology, Pathophysiology and Immunology, Medical University of Vienna, Vienna, Austria
    J Immunol 182:2160-7. 2009
    The current model for regulation of the Src family kinase member Lck postulates a strict correlation between structural condensation of the kinase backbone and catalytic activity...
  16. ncbi Activation of phosphatidylinositol-3-kinase in Jurkat T cells depends on the presence of the p56lck tyrosine kinase
    M von Willebrand
    Division of Cell Biology, Institute for Allergy and Immunology, La Jolla, CA 92037
    Eur J Immunol 24:234-8. 1994
    ..tyrosine phosphorylation and activation of PI3K in Jurkat T leukemia cells depend on the presence of the p56lck tyrosine kinase: in a variant of the Jurkat T cell line lacking p56lck, JCaM1, these responses were absent...
  17. ncbi Regulation of the Src family kinase Lck by Hsp90 and ubiquitination
    Ana Giannini
    Department of Immunobiology, Guy s Hospital, King s College London, United Kingdom
    Mol Cell Biol 24:5667-76. 2004
    Regulation of the Src-related tyrosine kinase Lck is crucial to the outcome of T-cell receptor (TCR) stimulation. It was previously shown that the stability of the constitutively active mutant LckY505F is controlled by Hsp90 (M. J...
  18. ncbi Lck is required for stromal cell-derived factor 1 alpha (CXCL12)-induced lymphoid cell chemotaxis
    Marit Inngjerdingen
    Department of Anatomy, Institute of Basic Medical Sciences, University of Oslo, Norway
    Blood 99:4318-25. 2002
    ..Damnacanthal, a specific Lck inhibitor, but not the Syk inhibitor piceatannol, inhibited CXCL12-induced chemotaxis of both lymphocyte subsets...
  19. ncbi Proline residues in CD28 and the Src homology (SH)3 domain of Lck are required for T cell costimulation
    A D Holdorf
    Department of Pathology, Center for Immunology, Pulmonary Division, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Exp Med 190:375-84. 1999
    The Src family tyrosine kinases Lck and Fyn are critical for signaling via the T cell receptor. However, the exact mechanism of their activation is unknown...
  20. ncbi Identification of two SH3-binding motifs in the regulatory subunit of phosphatidylinositol 3-kinase
    R Kapeller
    Department of Physiology, Tufts University Medical School, Boston, Massachusetts 02111
    J Biol Chem 269:1927-33. 1994
    ..Here we show that p85, the regulatory subunit of PI 3-kinase, binds directly to the SH3 domains of Abl, Lck, Fyn, and p85 itself...
  21. ncbi Unc119, a novel activator of Lck/Fyn, is essential for T cell activation
    Magdalena M Gorska
    Division of Allergy and Immunology, Department of Internal Medicine, University of Texas Medical Branch, Galveston 77555, USA
    J Exp Med 199:369-79. 2004
    The first step in T cell receptor for antigen (TCR) signaling is the activation of the receptor-bound Src kinases, Lck and Fyn. The exact mechanism of this process is unknown...
  22. ncbi Selective interaction of LAT (linker of activated T cells) with the open-active form of Lck in lipid rafts reveals a new mechanism for the regulation of Lck in T cells
    Panagiotis S Kabouridis
    Bone and Joint Research Unit, Barts and the London School of Medicine and Dentistry, Queen Mary, University of London, Charterhouse Square, London EC1M 6BQ, UK
    Biochem J 371:907-15. 2003
    In T cells, the lipid raft-associated Lck is strongly tyrosine phosphorylated and has reduced enzymic activity in contrast with the detergent-soluble pool, which has substantial activity...
  23. ncbi Profound block in thymocyte development in mice lacking p56lck
    T J Molina
    Ontario Cancer Institute, University of Toronto, Canada
    Nature 357:161-4. 1992
    The protein Lck (p56lck) has a relative molecular mass of 56,000 and belongs to the Src family of tyrosine kinases. It is expressed exclusively in lymphoid cells, predominantly in thymocytes and peripheral T cells...
  24. ncbi A 10-aa-long sequence in SLP-76 upstream of the Gads binding site is essential for T cell development and function
    Lalit Kumar
    Division of Immunology, Children s Hospital, CBR Institute for Biomedical Research, Harvard Medical School, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 102:19063-8. 2005
    The adapter SLP-76 is essential for T cell development and function. SLP-76 binds to the src homology 3 domain of Lck in vitro. This interaction depends on amino acids 185-194 of SLP-76...
  25. ncbi Syk and ZAP-70 mediate recruitment of p56lck/CD4 to the activated T cell receptor/CD3/zeta complex
    M Thome
    Department of Immunology, Institut Pasteur, Paris, France
    J Exp Med 181:1997-2006. 1995
    ..suggested that the association of CD4 with TCR/CD3/zeta requires the interaction of the protein tyrosine kinase p56lck with CD4...
  26. ncbi Binding of HIV-1 to its receptor induces tyrosine phosphorylation of several CD4-associated proteins, including the phosphatidylinositol 3-kinase
    G Briand
    Centre de Recherche en Infectiologie, Centre Hospitalier Universitaire de Quebec, Canada
    Virology 228:171-9. 1997
    ..Immunoblot analysis permitted the identification of two of these proteins, p56lck and phosphatidylinositol 3-kinase (PI 3-kinase) p85 alpha...
  27. ncbi Modulation of Lck function through multisite docking to T cell-specific adapter protein
    Stine Granum
    Department of Anatomy, Institute of Basic Medical Sciences, University of Oslo, Box 1105, Blindern, N 0317 Oslo, Norway
    J Biol Chem 283:21909-19. 2008
    T cell-specific adapter protein (TSAd), encoded by the SH2D2A gene, interacts with Lck through its C terminus and thus modulates Lck activity...
  28. ncbi SHP-1 regulates Lck-induced phosphatidylinositol 3-kinase phosphorylation and activity
    B Cuevas
    Division of Medicine, and the Cell Growth Regulation Laboratory, University of Texas M D Anderson Cancer Center, Houston, Texas 77030, USA
    J Biol Chem 274:27583-9. 1999
    Ligation of the T cell antigen receptor (TCR) activates the Src family tyrosine kinase p56 Lck, which, in turn, phosphorylates a variety of intracellular substrates...
  29. ncbi Lad, an adapter protein interacting with the SH2 domain of p56lck, is required for T cell activation
    Y B Choi
    Signal Transduction Laboratory Mogam Biotechnology Research Institute, Koosungmyon, Yonginsi, Kyunggido, Korea
    J Immunol 163:5242-9. 1999
    T cell-specific Src family tyrosine kinase, p56lck, plays crucial roles in T cell differentiation, activation, and proliferation...
  30. ncbi Dok-3, a novel adapter molecule involved in the negative regulation of immunoreceptor signaling
    S Lemay
    McGill Cancer Centre, McGill University, Montreal, Quebec, Canada H3G 1Y6
    Mol Cell Biol 20:2743-54. 2000
    ....
  31. ncbi Phosphoprotein associated with glycosphingolipid-enriched microdomains (PAG), a novel ubiquitously expressed transmembrane adaptor protein, binds the protein tyrosine kinase csk and is involved in regulation of T cell activation
    T Brdicka
    Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, 14220 Prague, Czech Republic
    J Exp Med 191:1591-604. 2000
    ..These findings collectively suggest that in the absence of external stimuli, the PAG-Csk complex transmits negative regulatory signals and thus may help to keep resting T cells in a quiescent state...
  32. ncbi Evidence of LAT as a dual substrate for Lck and Syk in T lymphocytes
    Yixing Jiang
    Department of Medicine and Pennstate Cancer Institute, Penn State University College of Medicine, Hershey, PA 17033, United States
    Leuk Res 31:541-5. 2007
    ..In this study, we demonstrate that LAT serves as a dual substrate for both Lck and Syk kinases. LAT phosphorylation is absent in Lck-deficient J.CaM1...
  33. ncbi T cell receptor (TCR) interacting molecule (TRIM), a novel disulfide-linked dimer associated with the TCR-CD3-zeta complex, recruits intracellular signaling proteins to the plasma membrane
    E Bruyns
    Institute for Immunology, University of Heidelberg, 69120 Heidelberg, Germany
    J Exp Med 188:561-75. 1998
    ..Thus, TRIM represents a TCR-associated transmembrane adaptor protein which is likely involved in targeting of intracellular signaling proteins to the plasma membrane after triggering of the TCR...
  34. ncbi LCK-phosphorylated human killer cell-inhibitory receptors recruit and activate phosphatidylinositol 3-kinase
    F Marti
    The Immunology Program, Memorial Sloan Kettering Cancer Center, Cornell University Medical Center, New York, NY 10021, USA
    Proc Natl Acad Sci U S A 95:11810-5. 1998
    ..Using these systems, we show that KIR CYT, once phosphorylated by the src-family tyrosine kinase LCK, additionally bind the p85alpha regulatory subunit of phosphatidylinositol (PI) 3-kinase...
  35. ncbi The association of the protein tyrosine kinases p56lck and p60fyn with the glycosyl phosphatidylinositol-anchored proteins Thy-1 and CD48 in rat thymocytes is dependent on the state of cellular activation
    D Garnett
    MRC Cellular Immunology Unit, Sir William Dunn School of Pathology, University of Oxford, GB
    Eur J Immunol 23:2540-4. 1993
    ..are shown to be associated with multimolecular complexes of phosphoproteins including the protein tyrosine kinases p56lck and p60fyn in both rat and mouse thymocytes...
  36. ncbi Molecular cloning of SKAP55, a novel protein that associates with the protein tyrosine kinase p59fyn in human T-lymphocytes
    A Marie-Cardine
    Ruprecht Karls University Heidelberg, Institute of Immunology, 69120 Heidelberg, Germany
    J Biol Chem 272:16077-80. 1997
    ..Consistent with this observation, pp55 selectively binds to isolated SH2 domains of Lck, Lyn, Src, and Fyn but not to the SH2 domains of ZAP70, Syk, Shc, SLP-76, Grb2, phosphatidylinositol 3-kinase, and ..
  37. ncbi Modification of phosphatidylinositol 3-kinase SH2 domain binding properties by Abl- or Lck-mediated tyrosine phosphorylation at Tyr-688
    M von Willebrand
    Divisions of Cell Biology and Cellular Immunology, La Jolla Institute for Allergy and Immunology, San Diego, California 92121, USA
    J Biol Chem 273:3994-4000. 1998
    ..We report that this phosphorylation event is readily catalyzed by the Abl and Lck protein-tyrosine kinases in vitro, by Bcr-Abl or a catalytically activated Lck-Y505F in co-transfected COS cells, ..
  38. ncbi Regulation of Bcl-3 through interaction with the Lck tyrosine kinase
    Yujie Zhao
    Center for Advanced Biotechnology and Medicine, University of Medicine and Dentistry of New Jersey, Robert Wood Johnson Medical School, Piscataway, NJ 08854, USA
    Biochem Biophys Res Commun 335:865-73. 2005
    ..that Bcl-3 interacts with the Fyn tyrosine kinase in platelets, we investigated possible interactions of Bcl-3 with Lck, a related tyrosine kinase important in lymphoid cells...
  39. ncbi A membrane proximal domain of the human interleukin-3 receptor beta c subunit that signals DNA synthesis in NIH 3T3 cells specifically binds a complex of Src and Janus family tyrosine kinases and phosphatidylinositol 3-kinase
    P Rao
    Holland Laboratory for BioMedical Science, American Red Cross, Rockville, Maryland 20855
    J Biol Chem 270:6886-93. 1995
    ..Purified Lyn and Lck kinase, but not Fes, could phosphorylate tyrosines in both domains...
  40. ncbi Association between mitogen-activated protein kinase and the zeta chain of the T cell receptor (TcR) with the SH2,3 domain of p56lck. Differential regulation by TcR cross-linking
    A August
    Immunology Program, Sloan Kettering Institute for Cancer Research, New York, New York 10021, USA
    J Biol Chem 271:10054-9. 1996
    A number of protein-tyrosine kinases have been shown to be important in T cell activation. One such kinase, Lck, has been demonstrated genetically to be essential for T cell receptor (TcR) signaling, and the SH2 and SH3 (src homology 2 ..
  41. ncbi Sequential involvement of Lck and SHP-1 with MHC-recognizing receptors on NK cells inhibits FcR-initiated tyrosine kinase activation
    B A Binstadt
    Department of Immunology, Mayo Clinic and Foundation, Rochester, Minnesota 55905, USA
    Immunity 5:629-38. 1996
    ..Using a somatic genetic model, we first define a requirement for the Src family protein tyrosine kinase (PTK) Lck in mediating KIR tyrosine phosphorylation...
  42. ncbi Activation of rabbit platelets by Ca2+ influx and thromboxane A2 release in an external Ca(2+)-dependent manner by zooxanthellatoxin-A, a novel polyol
    M C Rho
    Department of Pharmaceutical Molecular Biology, Faculty of Pharmaceutical Sciences, Tohoku University, Sendai, Japan
    Br J Pharmacol 115:433-40. 1995
    ..The resulting increase in [Ca2+]i subsequently stimulates the secondary release of TXA2 from platelets. Furthermore, the response to ZT-A may be associated with tyrosine phosphorylation...
  43. ncbi Detection of a physical and functional interaction between Csk and Lck which involves the SH2 domain of Csk and is mediated by autophosphorylation of Lck on tyrosine 394
    C Bougeret
    INSERM, , France
    J Biol Chem 271:7465-72. 1996
    ..analysis (BIAcore(TM)), we detected in vitro a specific interaction between Csk and one of its substrates Lck, a lymphocyte-specific member of the Src family...
  44. ncbi Signaling mechanisms of basic fibroblast growth factor in arterial cells from genetically hypertensive rat
    D L Zhu
    CNRS URA 1482, Laboratoire de Pharmacologie, , Paris, France
    Am J Hypertens 7:351-6. 1994
    ..Therefore, one may envisage that bFGF contributes, through paracrine/autocrine mechanisms, to the vascular smooth muscle hyperplasia/hypertrophy in SHR...
  45. ncbi Platelet-derived growth factor. Structure, function and implications in normal and malignant cell growth
    B Westermark
    Department of Pathology, University Hospital, Uppsala, Sweden
    Acta Oncol 32:101-5. 1993
    ..This view is supported by our recent finding that human melanoma cells that have been stably transfected with a PDGF B-chain cDNA, elicit a stroma response when transplanted to nude mice...
  46. ncbi Protein tyrosine kinase in colorectal adenoma
    T Hatada
    Department of Surgery, Hyogo College of Medicine, Japan
    Acta Oncol 32:23-8. 1993
    ....
  47. ncbi Protein tyrosine kinase activity in human thyroid papillary carcinoma
    Y Yasuda
    Second Department of Surgery, Hyogo College of Medicine, Japan
    Acta Oncol 31:409-12. 1992
    ..These findings indicate that elevated cytosolic PTK activity may be a potentially useful marker for papillary thyroid carcinoma...
  48. ncbi Activation of p58c-fgr and p53/56lyn in adherent human neutrophils: evidence for a role of divalent cations in regulating neutrophil adhesion and protein tyrosine kinase activities
    S R Yan
    Institute of General Pathology, University of Verona, Italy
    J Inflamm 45:297-311. 1995
    ....
  49. ncbi Effect of hypoxia on protein tyrosine kinase activity in cortical membranes of newborn piglets--the role of nitric oxide
    Om Prakash Mishra
    Department of Pediatrics, Neonatal Research Laboratory, Drexel University College of Medicine, MCP, Room 701, 7th Floor Heritage Building, 3300 Henry Avenue, Philadelphia, PA 19129, USA
    Neurosci Lett 372:114-8. 2004
    ..We propose that the hypoxia-induced increase in protein tyrosine kinase activity leading to increased phosphorylation of Bcl-2 is a critical link to hypoxic neuronal injury pathway...
  50. ncbi Demonstration of selective protein kinase C-dependent activation of Src and Lck tyrosine kinases during ischemic preconditioning in conscious rabbits
    P Ping
    Experimental Research Laboratory, Division of Cardiology and the Department of Physiology and Biophysics, University of Louisville and Jewish Hospital Heart and Lung Institute, KY 40202, USA
    Circ Res 85:542-50. 1999
    ..and 4-minute reperfusion) induces selective activation of 2 members of the Src family of tyrosine kinases, Src and Lck, in the heart of conscious rabbits...
  51. ncbi L-selectin activates the Ras pathway via the tyrosine kinase p56lck
    B Brenner
    Department of Pediatrics, University of Heidelberg, Germany
    Proc Natl Acad Sci U S A 93:15376-81. 1996
    ..of Jurkat cells using different antibodies or glycomimetics resulted in activation of the src-tyrosine kinase p56lck; tyrosine phosphorylation of intracellular proteins, in particular mitogen-activating protein kinase and L-..
  52. ncbi Effects of the novel and potent lymphocyte-specific protein tyrosine kinase inhibitor TKM0150 on mixed lymphocyte reaction and contact hypersensitivity in mice
    Tetsuo Takayama
    Medicinal Research Laboratories, Taisho Pharmaceutical Co, Ltd, Saitama, Japan
    Arzneimittelforschung 60:282-5. 2010
    Lymphocyte-specific protein tyrosine kinase (Lck) plays a critical role in T cell activation...
  53. ncbi Calcium-induced ERK activation in human T lymphocytes occurs via p56(Lck) and CaM-kinase
    R A Franklin
    Department of Microbiology and Immunology, East Carolina University School of Medicine, Brody Building, Greenville, NC 27858, USA
    Mol Immunol 37:675-83. 2000
    ..of T lymphocytes with ionomycin or A23187 resulted in a CaM-kinase-dependent shift in the mobility of p56(Lck)...
  54. ncbi Properties of tripartite chimeras between Src and Lck
    A Kashishian
    Fred Hutchinson Cancer Research Center, Seattle, Washington 98104
    Oncogene 5:1463-70. 1990
    ..domains of Src-family kinases, we have assembled five chimeric molecules containing parts of p60c-src (Src) and p56lck (Lck)...
  55. ncbi Differential effects of expression of the CD45 tyrosine protein phosphatase on the tyrosine phosphorylation of the lck, fyn, and c-src tyrosine protein kinases
    T R Hurley
    Molecular Biology and Virology Laboratory, Salk Institute, San Diego, California 92186
    Mol Cell Biol 13:1651-6. 1993
    ..We have studied the phosphorylation of the closely related lck, fyn, and c-src tyrosine protein kinases in leukemic murine T-cell lines that have lost the expression of CD45...
  56. ncbi Partial purification and characterization of the lck protein-tyrosine kinase from bovine thymus
    Q M Wang
    Department of Medicinal Chemistry and Pharmacognosy, School of Pharmacy, Purdue University, West Lafayette, IN 47907
    Biochem J 279:567-74. 1991
    ..were also recognized by polyclonal anti-peptide antibodies raised against the C-terminal 33 amino acids of p56lck, a major T lymphocyte protein-tyrosine kinase...
  57. ncbi Zap70 signaling pathway mediates glucocorticoid receptor-dependent transcriptional activation: role in the regulation of annexin 1 expression in T cells
    Mohammad Ishaq
    Laboratory of Molecular Cell Biology, SAIC Frederick, National Cancer Institute, Frederick, MD 21702, USA
    J Immunol 179:3851-8. 2007
    ..b>Lck-lacking Jurkat cells were also found to show markedly reduced GR activation, and reconstitution with Lck restored ..
  58. ncbi Neocortical neurons lacking the protein-tyrosine kinase B receptor display abnormal differentiation and process elongation in vitro and in vivo
    M A Gates
    Division of Neuroscience, Children s Hospital, Harvard Medical School, Boston, MA 02115, USA
    Neuroscience 98:437-47. 2000
    ....
  59. ncbi Regulation of eicosanoid biosynthesis in the macrophage. Involvement of protein tyrosine phosphorylation and modulation by selective protein tyrosine kinase inhibitors
    K B Glaser
    Division of Immunopharmacology, Wyeth Ayerst Research, Princeton, NJ 08543 8000
    Biochem Pharmacol 45:711-21. 1993
    ..The use of selective PTK inhibitors suggests a common role for PTK and tyrosine phosphorylation in eicosanoid biosynthesis in the murine peritoneal macrophage...
  60. ncbi Differential regulation of fyn-associated protein tyrosine kinase activity by macrophage colony-stimulating factor (M-CSF) and granulocyte-macrophage colony-stimulating factor (GM-CSF)
    Y Li
    Department of Internal Medicine, Wayne State University School of Medicine, Detroit, Michigan 48201
    J Leukoc Biol 57:484-90. 1995
    ..Collectively, our data suggest that the activation of fyn kinase is closely associated with the acquisition of adherent capacity in maturing macrophages...
  61. ncbi Differential regulation of T cell receptor-mediated Th1 cell IFN-gamma production and proliferation by divergent cAMP-mediated redox pathways
    R Cochrane
    Department of Pathology, The University of Connecticut Health Center, Farmington, CT 06030 3105, USA
    J Interferon Cytokine Res 21:797-807. 2001
    ..Immunoblotting of lysates from an FSK-treated Th1 clone with antibodies to a carboxy-terminal epitope of p56(lck), a signal transduction enzyme upstream from ERK-1 and ERK2, did not detect p56(lck) unless the lysates were ..
  62. ncbi Transcriptional activation of lck by retrovirus promoter insertion between two lymphoid-specific promoters
    H T Adler
    Molecular Biology and Virology Laboratory, Salk Institute, San Diego, California 92138
    J Virol 62:4113-22. 1988
    b>p56lck, a member of the src family of cytoplasmic tyrosine protein kinases, is expressed primarily in lymphoid cells...
  63. ncbi Increased ubiquitination and reduced expression of LCK in T lymphocytes from patients with systemic lupus erythematosus
    Elizabeth C Jury
    Royal Free and University College Medical School, University College, London, UK
    Arthritis Rheum 48:1343-54. 2003
    ..To explore regulation of proximal signaling and composition of lipid rafts in T lymphocytes from patients with systemic lupus erythematosus (SLE)...
  64. ncbi Association of herpesvirus saimiri tip with lipid raft is essential for downregulation of T-cell receptor and CD4 coreceptor
    Nam Hyuk Cho
    Department of Microbiology and Molecular Genetics and Tumor Virology Division, New England Primate Research Center, Harvard Medical School, Southborough, Massachusetts 01772 9102, USA
    J Virol 80:108-18. 2006
    ..HVS), which is a T-lymphotropic tumor virus, is constitutively targeted to lipid rafts and interacts with cellular Lck tyrosine kinase and p80 WD repeat-containing endosomal protein...
  65. ncbi The cytoplasmic domain of CD4 is sufficient for its down-regulation from the cell surface by human immunodeficiency virus type 1 Nef
    S J Anderson
    Department of Virology and Molecular Biology, St Jude Children s Research Hospital, Memphis, Tennessee 38101
    J Virol 68:3092-101. 1994
    ..Since the cytoplasmic domain of CD4 is also the site of its association with p56lck, we used a series of CD4 mutants to determine whether the regions of the cytoplasmic domain of CD4 required for ..
  66. ncbi Human airway epithelial cells stimulate T-lymphocyte lck and fyn tyrosine kinase
    T H Kalb
    Department of Medicine, Mt Sinai Medical Center, New York, New York 10029, USA
    Am J Respir Cell Mol Biol 17:561-70. 1997
    ..To determine whether CD3 associated p59fyn, or CD4 and CD8 associated p56lck phosphotyrosine kinases (PTK) were involved, we assayed kinase activity in lymphocyte lysates immunoprecipitated ..
  67. ncbi The reproductive activity in the testis of Podarcis s. sicula involves D-aspartic acid: a study on c-kit receptor protein, tyrosine kinase activity and PCNA protein during annual sexual cycle
    Franca Raucci
    Department of Life Sciences, Second University of Naples, Via Vivaldi 43, 81100 Caserta, Italy
    Gen Comp Endocrinol 161:373-83. 2009
    ..sicula, suggesting that a sequential cascade of a functional relationship between testosterone levels, c-kit receptor protein, tyrosine kinase activity and PCNA could be partly mediated by D-aspartic acid...
  68. ncbi Effects of magnesium sulfate administration during hypoxia on CaM kinase IV and protein tyrosine kinase activities in the cerebral cortex of newborn piglets
    Ahmed G Mami
    Department of Pediatric Surgery, Drexel University College of Medicine and St Christopher s Hospital for Children, Philadelphia, PA 19129, USA
    Neurochem Res 31:57-62. 2006
    ..As a result, phosphorylation of CREB and Bcl-2 family of proteins is prevented leading to prevention of programmed cell death...
  69. ncbi JAK3 protein tyrosine kinase mediates interleukin-7-induced activation of phosphatidylinositol-3' kinase
    N Sharfe
    Division of Immunology and Allergy, Hospital for Sick Children, University of Toronto, Ontario, Canada
    Blood 86:2077-85. 1995
    ..IL-7-induced activation of three cyoplasmic tyrosine kinases in T cells, Jak1, Jak3, and the src-like kinase p56lck. Many members of the cytokine receptor superfamily activate the Jak protein tyrosine kinase family, with resultant ..
  70. ncbi Selective inhibition of the platelet-derived growth factor signal transduction pathway by a protein-tyrosine kinase inhibitor of the 2-phenylaminopyrimidine class
    E Buchdunger
    CIBA Pharmaceuticals Division, Oncology Research Department, Ciba Geigy Limited, Basel, Switzerland
    Proc Natl Acad Sci U S A 92:2558-62. 1995
    ..These findings suggest that CGP 53716 may have therapeutic potential for the treatment of diseases involving abnormal cellular proliferation induced by PDGF receptor activation...
  71. ncbi Association between Lyn protein tyrosine kinase (p53/56lyn) and the beta subunit of the granulocyte-macrophage colony-stimulating factor (GM-CSF) receptors in a GM-CSF-dependent human megakaryocytic leukemia cell line (M-07e)
    Y Li
    Department of Internal Medicine, Wayne State University School of Medicine, Detroit, MI 48201, USA
    J Immunol 155:2165-74. 1995
    ....
  72. ncbi Protein tyrosine kinase-dependent release of intracellular calcium in the sea urchin egg
    S S Shen
    Department of Zoology and Genetics, Iowa State University, Ames 50011 3223, USA
    Dev Growth Differ 41:345-55. 1999
    ..Therefore, it was concluded that sea urchin eggs contain a PTK-dependent pathway that can mediate intracellular Ca2+ release, but PTK activity does not appear to be required for the fertilization response...
  73. ncbi Two lck transcripts containing different 5' untranslated regions are present in T cells
    A F Voronova
    Molecular Biology and Virology Laboratory, Salk Institute, San Diego, California 92138
    Mol Cell Biol 7:4407-13. 1987
    b>p56lck is a new member of the src family of cellular tyrosine protein kinases...
  74. ncbi The structure-activity relationship of the series of non-peptide small antagonists for p56lck SH2 domain
    See Hyoung Park
    Signal Transduction Laboratory, Mogam Biotechnology Research Institute, 341 Pojung Ri, Koosung Myun, Yongin City, Kyunggi Do, 449 910, Republic of Korea
    Bioorg Med Chem 15:3938-50. 2007
    ..acid (alpha-o-caffeoyl-3,4-dihydroxyphenyl-lactic acid; RosA) from Prunella vulgaris as an antagonist for the p56lck SH2 domain by screening natural products...
  75. ncbi Interleukin 2 regulates the activity of the lyn protein-tyrosine kinase in a B-cell line
    T Torigoe
    Department of Pathology and Laboratory Medicine, University of Pennsylvania School of Medicine, Philadelphia 19104 6082
    Proc Natl Acad Sci U S A 89:2674-8. 1992
    Recently, interleukin 2 (IL-2) has been shown to induce increased activity of the p56lck protein-tyrosine kinase (PTK) in T-cell and natural killer cell lines, and evidence for a direct interaction between the p75 subunit of the IL-2 ..
  76. ncbi Signal transducer and activator of transcription 5b: a new target of breast tumor kinase/protein tyrosine kinase 6
    Amanda M Weaver
    Department of Microbiology, University of Virginia, Charlottesville, VA 22908, USA
    Breast Cancer Res 9:R79. 2007
    ..Only a few substrates of the Brk tyrosine kinase have been identified, the most recent being STAT3. In the present article we investigate the potential role of Brk in the phosphorylation and activation STAT5b...
  77. ncbi [Effects of matrine on the activity of protein tyrosine kinase and phosphatase in K562 cells]
    Bei Zhong Liu
    Department of Clinical Biochemistry, Chongqing Medical University, Chongqing 400016, P R China
    Ai Zheng 21:1292-5. 2002
    ..The differentiation of K562 cells can be induced by a given concentration of matrine. This study was designed to investigate the mechanism of signal transduction in induced differentiation of K562 cells...
  78. ncbi Protein-tyrosine kinase CAKbeta/PYK2 is activated by binding Ca2+/calmodulin to FERM F2 alpha2 helix and thus forming its dimer
    Takayuki Kohno
    The Department of Biochemistry, Cancer Research Institute, Sapporo Medical University School of Medicine, South 1, West 17, Chuo Ku, Sapporo 060 8556, Japan
    Biochem J 410:513-23. 2008
    ..Our results indicate that Ca2+/calmodulin binding to the FERM F2-alpha2 helix of CAKbeta/PYK2 releases its kinase domain from autoinhibition by forming a dimer...
  79. ncbi The protein tyrosine kinase p56lck regulates TCR expression and T cell selection
    P O Ericsson
    Department of Microbiology and Immunology, University of British Columbia, Vancouver, Canada
    Int Immunol 7:617-24. 1995
    The role of the protein tyrosine kinase (PTK), p56lck, in T cell development was evaluated by mating TCR transgenic mice with transgenic mice that expressed lckF505, a constitutively activated form of p56lck which is under the control of ..
  80. ncbi Matrix metalloproteinase-2 and -9 are induced differently by metal nanoparticles in human monocytes: The role of oxidative stress and protein tyrosine kinase activation
    Rong Wan
    Department of Environmental Health and Occupational Health Sciences, School of Public Health and Information Sciences, University of Louisville, 555 South Floyd Street, Louisville, KY 40202, USA
    Toxicol Appl Pharmacol 233:276-85. 2008
    ..Our results suggest that Nano-Co causes an imbalance between the expression and activity of MMPs and their inhibitors which is mediated by the AP-1 and tyrosine kinase pathways due to oxidative stress...
  81. ncbi Solution structure and backbone dynamics of the non-receptor protein-tyrosine kinase-6 Src homology 2 domain
    Eunmi Hong
    Department of Biochemistry and Protein Network Research Center, College of Science, Yonsei University, Seoul 120 749, Korea
    J Biol Chem 279:29700-8. 2004
    ..The solution structure together with data from the ligand binding mode of PTK6-SH2 provides insight into the molecular basis of the autoinhibitory role of PTK6...
  82. ncbi Organization of the human PTK7 gene encoding a receptor protein tyrosine kinase-like molecule and alternative splicing of its mRNA
    Jae Won Jung
    National Research Laboratory of Cellular Biochemistry, Department of Biochemistry, College of Science, and Protein Network Research Center, Yonsei University, Seoul, South Korea
    Biochim Biophys Acta 1579:153-63. 2002
    ..Our findings suggest that PTK7 is evolutionarily distinct from other RPTKs, and that the alternative splicing of PTK7 mRNA may contribute to its diverse function in cell signaling...
  83. ncbi Silica-induced nuclear factor-kappaB activation: involvement of reactive oxygen species and protein tyrosine kinase activation
    J L Kang
    Department of Physiology, College of Medicine, Ewha Medical Research Center, Ewha Womans University, Seoul, Korea
    J Toxicol Environ Health A 60:27-46. 2000
    ..The results suggest that ROS may play a role in silica-induced NF-kappaB activation in macrophages and that phosphorylation events mediated by tyrosine kinase may be involved in this activation...
  84. ncbi Mechanism of desensitization of the epidermal growth factor receptor protein-tyrosine kinase
    J L Countaway
    Howard Hughes Medical Institute, University of Massachusetts Medical School, Worcester 01605
    J Biol Chem 267:1129-40. 1992
    ..This regulatory phosphorylation site is located at the carboxyl terminus of the EGF receptor within the subdomain that binds src homology 2 regions of signaling molecules...
  85. ncbi Generation of an active protein-tyrosine kinase from lymphocytes by proteolysis
    T F Zioncheck
    Department of Medicinal Chemistry and Pharmacognosy, School of Pharmacy and Pharmacal Sciences, Purdue University, West Lafayette, Indiana 47907
    J Biol Chem 263:19195-202. 1988
    ..p72 is also found in certain T and B cell-derived cell lines and in NIH3T3 cells...
  86. ncbi Regulation of the human c-fes protein tyrosine kinase (p93c-fes) by its src homology 2 domain and major autophosphorylation site (Tyr-713)
    S J Hjermstad
    Eppley Institute for Research in Cancer, University of Nebraska Medical Center, Omaha 68198 6805
    Oncogene 8:2283-92. 1993
    ..These results indicate that the c-fes SH2 domain and consensus autophosphorylation site (Tyr-713) play major roles in the positive regulation of p93c-fes tyrosine kinase activity, possibly through intramolecular interaction...
  87. ncbi Frequent activation of the lck gene by promoter insertion and aberrant splicing in murine leukemia virus-induced rat lymphomas
    S Shin
    Division of Molecular Virology, Baylor College of Medicine, Houston, Texas 77030
    Oncogene 8:141-9. 1993
    ..in Fischer rats by Moloney murine leukemia virus for alterations affecting the structure or expression of the lck gene. At least five primary tumors (14%) have a proviral insertion upstream of lck...
  88. ncbi Structural analysis of the lymphocyte-specific kinase Lck in complex with non-selective and Src family selective kinase inhibitors
    X Zhu
    Kinetix Pharmaceuticals, Inc, Medford, MA 02155, USA
    Structure 7:651-61. 1999
    The lymphocyte-specific kinase Lck is a member of the Src family of non-receptor tyrosine kinases. Lck catalyzes the initial phosphorylation of T-cell receptor components that is necessary for signal transduction and T-cell activation...
  89. ncbi Retinal dysplasia in mice lacking p56lck
    B Omri
    CNRS UPR 9035, Paris, France
    Oncogene 16:2351-6. 1998
    The product of the proto-oncogene p56lck is a non-receptor tyrosine kinase member of the Src family. It is found in T cells (Marth et al., 1985, 1988) and in the mouse brain (Omri et al., 1996; Van Tan et al., 1996)...
  90. ncbi The diabetic BB rat. Neither Th1 nor Th2?
    D Bellgrau
    Barbara Davis Center for Childhood Diabetes, University of Colorado Health Sciences Center, Denver, USA
    Horm Metab Res 28:299-301. 1996
    ..Our data also indicate that transcript expression for p56lck, an enzyme required for T cell development, is abnormal in BB peripheral T cells...
  91. ncbi Hypophosphorylated TCR/CD3zeta signals through a Grb2-SOS1-Ras pathway in Lck knockdown cells
    Trond Methi
    The Biotechnology Centre of Oslo, University of Oslo, Oslo, Norway
    Eur J Immunol 37:2539-48. 2007
    ..signaling events, downstream T cell responses are paradoxically augmented in T cells with siRNA-mediated Lck knockdown (Methi et al., J. Immunol. 2005. 175: 7398-7406)...
  92. ncbi Inhibition of p56(lck) tyrosine kinase by isothiazolones
    J M Trevillyan
    Abbott Laboratories, Immunological Disease Research, Abbott Park, Illinois, 60064 6119, USA
    Arch Biochem Biophys 364:19-29. 1999
    b>Lck encodes a 56-kDa protein-tyrosine kinase, predominantly expressed in T lymphocytes, crucial for initiating T cell antigen receptor (TCR) signal transduction pathways, culminating in T cell cytokine gene expression and effector ..
  93. ncbi Effect of dietary K intake on apical small-conductance K channel in CCD: role of protein tyrosine kinase
    Y Wei
    Department of Pharmacology, New York Medical College, Valhalla, New York 10595, USA
    Am J Physiol Renal Physiol 281:F206-12. 2001
    ..We conclude that dietary K intake plays a key role in regulating the activity of the SK channels and that PTK is involved in mediating the effect of the K intake on channel activity in the CCD...
  94. ncbi The protein-tyrosine kinase Lck associates with and is phosphorylated by Cdc2
    N I Pathan
    Department of Biology, Purdue University, West Lafayette, Indiana 47907, USA
    J Biol Chem 271:27517-23. 1996
    The protein-tyrosine kinase Lck is essential for signaling through the T-cell antigen receptor. Treatment of T-cells with a variety of extracellular stimuli increases the phosphorylation of Lck on serine residues...
  95. ncbi Analysis of CD28 cytoplasmic tail tyrosine residues as regulators and substrates for the protein tyrosine kinases, EMT and LCK
    P D King
    The Immunology Program, Sloan Kettering Institute for Cancer Research, New York 10021, USA
    J Immunol 158:580-90. 1997
    ..and activation of phosphatidylinositol 3-kinase (PI3-kinase) and activation of the protein tyrosine kinases (PTKs), LCK and EMT...
  96. ncbi Expression of p56lck in B-cell neoplasias
    A von Knethen
    Klinik IV für Nephrologie, Universitat Erlangen, Germany
    Leuk Lymphoma 26:551-62. 1997
    The protooncogene p56lck is considered to participate in malignant transformation of lymphoid cells...
  97. ncbi Src tyrosine kinase augments taxotere-induced apoptosis through enhanced expression and phosphorylation of Bcl-2
    V Boudny
    Department of Medicine and Biosystemic Science, Graduate School of Medicine, Kyushu University, 3 1 1 Maidashi, Higashi ku, Fukuoka, Fukuoka 812 8582, Japan
    Br J Cancer 86:463-9. 2002
    ....
  98. ncbi Characterization of the interactions between the active site of a protein tyrosine kinase and a divalent metal activator
    Xiaofeng Lin
    Department of Cell and Molecular Biology, University of Rhode Island, Kingston, RI 02881, USA
    BMC Biochem 6:25. 2005
    ..Protein tyrosine kinase Csk requires two Mg2+ cations for activity: one (M1) binds to ATP, and the other (M2) acts as an essential activator...
  99. ncbi Role of lymphocyte-specific protein tyrosine kinase (LCK) in the expansion of glioma-initiating cells by fractionated radiation
    Rae Kwon Kim
    Department of Chemistry, Research Institute for Natural Sciences, Hanyang University, Seoul, South Korea
    Biochem Biophys Res Commun 402:631-6. 2010
    ..In this study, we show that LCK (lymphocyte-specific protein tyrosine kinase) is involved in the fractionated radiation-induced expansion of the ..
  100. ncbi The c-Fes protein-tyrosine kinase suppresses cytokine-independent outgrowth of myeloid leukemia cells induced by Bcr-Abl
    J M Lionberger
    Eppley Institute for Research in Cancer and Department of Pathology and Microbiology University of Nebraska Medical Center, Omaha 68198, USA
    Cancer Res 60:1097-103. 2000
    ..These results show that Fes and Bcr-Abl interact in myeloid cells, leading to Fes activation and suppression of Bcr-Abl-induced conversion to cytokine independence...
  101. ncbi The role of p56lck in the development of gamma delta T cells and their function during an infection by Listeria monocytogenes
    S Fujise
    Department of Immunology, Medical Institute of Bioregulation, Kyusyu University, Fukuoka, Japan
    J Immunol 157:247-54. 1996
    We investigated roles of p56lck tyrosine kinase (Lck) on the development and function of gamma delta T cells in adult mice using lck gene knockout (lck -/-) mice...

Research Grants73

  1. Genetic and Immunological Impact of the HRES-1/Rab4 Locus in SLE
    Andras Perl; Fiscal Year: 2010
    ..The key intracellular transducer of T-cell activation, Lck, is brought to the IS via binding to CD4. TCR6 chain binds to the TFR...
  2. p56lck Inhibitors as Potential Immunosuppressive Drugs.
    ALEXANDER MACKERELL; Fiscal Year: 2004
    SH2 domains in non-receptor tyrosine kinases, such as p56lck, represent molecular targets that are central to cellular signaling pathways related to immune response...
  3. TYROSINE PROTEIN KINASES AND LYMPHOCYTE ACTIVATION
    ROBERT GEAHLEN; Fiscal Year: 1993
    ..It is likely that the molecular mechanisms of signal transduction in B lymphocytes will serve as a prototype for receptor signaling pathways in a variety of cell types...
  4. TYROSINE PROTEIN KINASES AND LYMPHOCYTE ACTIVATION
    ROBERT GEAHLEN; Fiscal Year: 1991
    ..It is our contention that a greater understanding of the biochemical pathways that regulate normal cell growth and development is necessary to begin to define the lesions within this pathway that can lead to malignant growth...
  5. Role of Src Kinases in T Cell Receptor Signaling
    David Straus; Fiscal Year: 2004
    ..We will take advantage of our ability to express mutants of the Src-family kinase Lck in an Lck-deficient T cell line to determine what steps in the activation process are dependent upon particular ..
  6. Negative regulation of TCR-associated PTKs
    Tomas Mustelin; Fiscal Year: 2006
    ..We will determine to what extent TCR signaling is altered in the absence of PEP and if Lck and Fyn are hyperactive...
  7. Negative regulation of TCR-associated PTKs
    ROBERT RICKERT; Fiscal Year: 2007
    ..We will determine to what extent TCR signaling is altered in the absence of PEP and if Lck and Fyn are hyperactive...
  8. Src Family Kinases as Molecular Targets for HIV/Nef
    THOMAS SMITHGALL; Fiscal Year: 2007
    ..Our work is focused on the interaction of Nef with protein kinases in infected cells. Completion of these experiments will validate these Nef-kinase complexes as targets for the discovery of new anti-HIV therapeutics. ..
  9. Src Family Kinases as Molecular Targets for HIV/Nef
    THOMAS EDWARD SMITHGALL; Fiscal Year: 2010
    ..Our work is focused on the interaction of Nef with protein kinases in infected cells. Completion of these experiments will validate these Nef-kinase complexes as targets for the discovery of new anti-HIV therapeutics. ..
  10. Phosphoproteomic Analysis of T Cell Activation Pathways
    ARTHUR ROBERT SALOMON; Fiscal Year: 2010
    ..within the pathway through quantitative phosphoproteomic analysis of T cells with disrupted pathway proteins LCK, PLC1, VAV, and ERK...
  11. OSTEOCLASTIC PHOSPHOTYROSYL PHOSPHATASE AND RESORPTION
    KIN HING LAU; Fiscal Year: 2002
    ....
  12. Focal Adhesion Kinase - Tumor Biology and Therapeutics
    William G Cance; Fiscal Year: 2010
    ..Thus, this application will provide novel molecular cancer therapeutics that target the focal adhesion kinase. ..
  13. INTERFERON SIGNAL TRANSDUCTION IN T CELLS
    Andrew Larner; Fiscal Year: 2001
    ..a rapid association with the alpha chain of the IFNalpha receptor (IFNalphaR1) of the protein tyrosine kinases Lck and ZAP-70 and the protein tyrosine phosphatase, CD-45...
  14. SIGNAL TRANSDUCTION IN T LYMPHOCYTE ACTIVATION
    Amnon Altman; Fiscal Year: 1993
    ..the following approaches will be undertaken to elucidate the roles of two T cell-expressed TPKs, p59fyn and p56lck, that are associated with the TCR/CD3 complex or the CD4/CD8 coreceptors, respectively, in T cell signaling, in ..
  15. PROTEIN TYROSINE KINASE TYRO-3
    ANNE PRIETO; Fiscal Year: 2003
    ..Through these efforts we hope to uncover the role of Tyro-3 in molecular mechanisms of cell adhesion, intracellular signaling and neuroplasticity in the normal and diseases nervous system. ..
  16. REGULATION OF BCR MEDIATED SIGNAL TRANSDUCTION BY CD45
    LOUIS JUSTEMENT; Fiscal Year: 2002
    ..Thus, the studies proposed above may provide information that enhances our understanding of the events which result in aberrant activation of the B cell. ..
  17. T cell regulation by N-glycosylation
    Michael Demetriou; Fiscal Year: 2010
    ..This in turn blocks recruitment of Nck, WASp, SLP-76 and CD4-Lck to TCR, F-actin remodeling and transfer of the complex to GM1/cholesterol-enriched microdomains (GEMs)...
  18. T cell regulation by N-glycosylation
    Michael Demetriou; Fiscal Year: 2010
    ..This in turn blocks recruitment of Nck, WASp, SLP-76 and CD4-Lck to TCR, F-actin remodeling and transfer of the complex to GM1/cholesterol-enriched microdomains (GEMs)...
  19. Mechanisms of Breast Tumor Cell Growth, Motility, and Antiestrogen Response
    Amy Bouton; Fiscal Year: 2009
    ....
  20. Mechanisms of Breast Tumor Cell Growth, Motility, and Antiestrogen Response
    Amy H Bouton; Fiscal Year: 2010
    ....
  21. A Tumor Suppressor Function for the c-Fes Tyrosine Kinase in Colon Cancer
    THOMAS EDWARD SMITHGALL; Fiscal Year: 2010
    ..If c-fes is a true tumor suppressor, then mice lacking c-fes function are expected to develop more severe disease in terms of tumor size, number, and invasiveness. ..
  22. MITOGENIC SIGNALING BY ENDOTHELIN PEPTIDES
    Michael Simonson; Fiscal Year: 1993
    ..Because ET-1 has been implicated the vascular response to injury and in renal failure, the ability to block the mitogenic actions of ET might be relevant in clinical settings...