Ku70

Summary

Gene Symbol: Ku70
Description: X-ray repair cross complementing 6
Alias: CTC75, CTCBF, G22P1, KU70, ML8, TLAA, X-ray repair cross-complementing protein 6, 5'-dRP lyase Ku70, 5'-deoxyribose-5-phosphate lyase Ku70, 70 kDa subunit of Ku antigen, ATP-dependent DNA helicase 2 subunit 1, ATP-dependent DNA helicase II, 70 kDa subunit, CTC box binding factor 75 kDa subunit, DNA repair protein XRCC6, Ku autoantigen p70 subunit, Ku autoantigen, 70kDa, X-ray repair complementing defective repair in Chinese hamster cells 6, lupus Ku autoantigen protein p70, thyroid autoantigen 70kD (Ku antigen), thyroid autoantigen 70kDa (Ku antigen), thyroid-lupus autoantigen p70
Species: human
Products:     Ku70

Top Publications

  1. Lan L, Ui A, Nakajima S, Hatakeyama K, Hoshi M, Watanabe R, et al. The ACF1 complex is required for DNA double-strand break repair in human cells. Mol Cell. 2010;40:976-87 pubmed publisher
    ..ACF1 interacts directly with KU70 and is required for the accumulation of KU proteins at DSBs...
  2. Mayeur G, Kung W, Martinez A, Izumiya C, Chen D, Kung H. Ku is a novel transcriptional recycling coactivator of the androgen receptor in prostate cancer cells. J Biol Chem. 2005;280:10827-33 pubmed
    ..AR directly interacts with both Ku70 and Ku80 in vivo and in vitro, as shown by co-immunoprecipitation, glutathione S-transferase pull-down, and Sf9 ..
  3. Fell V, Schild Poulter C. Ku regulates signaling to DNA damage response pathways through the Ku70 von Willebrand A domain. Mol Cell Biol. 2012;32:76-87 pubmed publisher
    The Ku heterodimer (Ku70/Ku80) is a main component of the nonhomologous end-joining (NHEJ) pathway that repairs DNA double-strand breaks (DSBs)...
  4. Nick McElhinny S, Snowden C, McCarville J, Ramsden D. Ku recruits the XRCC4-ligase IV complex to DNA ends. Mol Cell Biol. 2000;20:2996-3003 pubmed
    ..We have therefore defined cell-free conditions that reflect the genetic requirement for ligase IV in cellular end joining and consequently can explain in molecular terms why this factor is required. ..
  5. Gottlieb T, Jackson S. The DNA-dependent protein kinase: requirement for DNA ends and association with Ku antigen. Cell. 1993;72:131-42 pubmed
    ..The implications of the unusual DNA binding mode and multicomponent nature of the DNA-PK are discussed. ..
  6. Kang H, Lee Y, Shin J, Andrabi S, Chi Z, Gagné J, et al. Iduna is a poly(ADP-ribose) (PAR)-dependent E3 ubiquitin ligase that regulates DNA damage. Proc Natl Acad Sci U S A. 2011;108:14103-8 pubmed publisher
    ..that are either PARsylated or bind PAR including PAR polymerase-1, 2 (PARP1, 2), nucleolin, DNA ligase III, KU70, KU86, XRCC1, and histones...
  7. Mari P, Florea B, Persengiev S, Verkaik N, Bruggenwirth H, Modesti M, et al. Dynamic assembly of end-joining complexes requires interaction between Ku70/80 and XRCC4. Proc Natl Acad Sci U S A. 2006;103:18597-602 pubmed
    ..Remarkably, the Ku heterodimers on DNA ends were in dynamic equilibrium with Ku70/80 in solution, showing that NHEJ complex assembly is reversible...
  8. Fu Y, Yu J, Cheng T, Lou M, Hsu G, Wu C, et al. Breast cancer risk associated with genotypic polymorphism of the nonhomologous end-joining genes: a multigenic study on cancer susceptibility. Cancer Res. 2003;63:2440-6 pubmed
    ..genetic variation in the genome, to examine these hypotheses, we have genotyped 30 SNPs in all five NHEJ genes (Ku70, Ku80, DNA-PKcs, Ligase IV, and XRCC4) in 254 primary breast cancer patients and 379 healthy controls...
  9. Li Y, Yokota T, Gama V, Yoshida T, Gomez J, Ishikawa K, et al. Bax-inhibiting peptide protects cells from polyglutamine toxicity caused by Ku70 acetylation. Cell Death Differ. 2007;14:2058-67 pubmed
    ..Here, we show that expanded polyQ of ataxin-3 (Q79C), a gene that causes MJD, stimulates Ku70 acetylation, which in turn dissociates the proapoptotic protein Bax from Ku70, thereby promoting Bax activation and ..

More Information

Publications69

  1. Taylor T, Knipe D. Proteomics of herpes simplex virus replication compartments: association of cellular DNA replication, repair, recombination, and chromatin remodeling proteins with ICP8. J Virol. 2004;78:5856-66 pubmed
    ..In Ku70-deficient murine embryonic fibroblasts, viral yields are increased by almost 50-fold, suggesting that the cellular ..
  2. Ruis B, Fattah K, Hendrickson E. The catalytic subunit of DNA-dependent protein kinase regulates proliferation, telomere length, and genomic stability in human somatic cells. Mol Cell Biol. 2008;28:6182-95 pubmed publisher
    ..protein kinase comprised of a 469-kDa catalytic subunit (DNA-PK(cs)) and the DNA binding regulatory heterodimeric (Ku70/Ku86) complex Ku...
  3. Kerr E, Holohan C, McLaughlin K, Majkut J, Dolan S, Redmond K, et al. Identification of an acetylation-dependant Ku70/FLIP complex that regulates FLIP expression and HDAC inhibitor-induced apoptosis. Cell Death Differ. 2012;19:1317-27 pubmed publisher
    ..We report a novel interaction between FLIP and the DNA repair protein Ku70 that regulates FLIP protein stability by inhibiting its polyubiquitination...
  4. Li B, Navarro S, Kasahara N, Comai L. Identification and biochemical characterization of a Werner's syndrome protein complex with Ku70/80 and poly(ADP-ribose) polymerase-1. J Biol Chem. 2004;279:13659-67 pubmed
    ..We have previously reported that WRN interacts with Ku70/80 and this interaction strongly stimulates WRN exonuclease activity...
  5. Song K, Jung D, Jung Y, Lee S, Lee I. Interaction of human Ku70 with TRF2. FEBS Lett. 2000;481:81-5 pubmed
    ..We have utilized the yeast two-hybrid system to identify Ku70-interacting proteins other than Ku80...
  6. Ko L, Chin W. Nuclear receptor coactivator thyroid hormone receptor-binding protein (TRBP) interacts with and stimulates its associated DNA-dependent protein kinase. J Biol Chem. 2003;278:11471-9 pubmed
    ..revealed a DNA-dependent protein kinase (DNA-PK) complex including its catalytic subunit and regulatory subunits, Ku70 and Ku86...
  7. Paddock M, Bauman A, Higdon R, Kolker E, Takeda S, Scharenberg A. Competition between PARP-1 and Ku70 control the decision between high-fidelity and mutagenic DNA repair. DNA Repair (Amst). 2011;10:338-43 pubmed publisher
    ..Here we show that immunoprecipitation of the BRCT domain of PARP-1 pulls down Ku70 and the DNA-PK complex although the BRCT domain of PARP-1 does not bind DNA, suggesting that this interaction is ..
  8. Trougakos I, Lourda M, Antonelou M, Kletsas D, Gorgoulis V, Papassideri I, et al. Intracellular clusterin inhibits mitochondrial apoptosis by suppressing p53-activating stress signals and stabilizing the cytosolic Ku70-Bax protein complex. Clin Cancer Res. 2009;15:48-59 pubmed publisher
    ..In the cytoplasm, sCLU depletion disrupts the Ku70-Bax complex and triggers Bax activation and relocation to mitochondria...
  9. Song K, Jung Y, Jung D, Lee I. Human Ku70 interacts with heterochromatin protein 1alpha. J Biol Chem. 2001;276:8321-7 pubmed
    ..It consists of a heterodimer of 70- and 80-kDa subunits. Recently we have demonstrated that Ku70 interacted with TRF2, a mammalian telomere-binding protein...
  10. Drouet J, Delteil C, Lefrançois J, Concannon P, Salles B, Calsou P. DNA-dependent protein kinase and XRCC4-DNA ligase IV mobilization in the cell in response to DNA double strand breaks. J Biol Chem. 2005;280:7060-9 pubmed
    ..DNA-PK comprises the Ku70/Ku80 heterodimer and the catalytic subunit DNA-PKcs...
  11. Yano K, Morotomi Yano K, Lee K, Chen D. Functional significance of the interaction with Ku in DNA double-strand break recognition of XLF. FEBS Lett. 2011;585:841-6 pubmed publisher
    ..These results demonstrate the significance of Ku-XLF interaction in the molecular assembly of NHEJ factors. ..
  12. Leskov K, Klokov D, Li J, Kinsella T, Boothman D. Synthesis and functional analyses of nuclear clusterin, a cell death protein. J Biol Chem. 2003;278:11590-600 pubmed
    Nuclear clusterin (nCLU) is an ionizing radiation (IR)-inducible protein that binds Ku70, and triggers apoptosis when overexpressed in MCF-7 cells. We demonstrate that endogenous nCLU synthesis is a product of alternative splicing...
  13. O Connor M, Safari A, Liu D, Qin J, Songyang Z. The human Rap1 protein complex and modulation of telomere length. J Biol Chem. 2004;279:28585-91 pubmed
    ..In addition to TRF2, we found DNA repair proteins Rad50, Mre11, PARP1 (poly(ADP-ribose) polymerase), and Ku86/Ku70 to be in this telomeric complex...
  14. Subramanian C, Jarzembowski J, Opipari A, Castle V, Kwok R. HDAC6 deacetylates Ku70 and regulates Ku70-Bax binding in neuroblastoma. Neoplasia. 2011;13:726-34 pubmed
    b>Ku70 was first characterized as a nuclear factor that binds DNA double-strand breaks in nonhomolog end-joining DNA repair...
  15. Warriar N, Page N, Govindan M. Expression of human glucocorticoid receptor gene and interaction of nuclear proteins with the transcriptional control element. J Biol Chem. 1996;271:18662-71 pubmed
    ..Both Ku80 and Ku70 polypeptides were present in high concentrations in human placenta and HeLa cells...
  16. Hu H, Zhang Y, Zou M, Yang S, Liang X. Expression of TRF1, TRF2, TIN2, TERT, KU70, and BRCA1 proteins is associated with telomere shortening and may contribute to multistage carcinogenesis of gastric cancer. J Cancer Res Clin Oncol. 2010;136:1407-14 pubmed publisher
    ..The expression of TRF1, TRF2, and TIN2 proteins was measured by Western blotting, while the expression of TERT, KU70, and BRCA1 proteins was detected using the immunohistochemical method...
  17. Zheng Y, Ao Z, Wang B, Jayappa K, Yao X. Host protein Ku70 binds and protects HIV-1 integrase from proteasomal degradation and is required for HIV replication. J Biol Chem. 2011;286:17722-35 pubmed publisher
    ..In the present study, we provide evidence that the host protein Ku70 interacts with HIV-1 IN and protects it from the Lys(48)-linked polyubiquitination proteasomal pathway...
  18. Wang H, Fang R, Cho J, Libermann T, Oettgen P. Positive and negative modulation of the transcriptional activity of the ETS factor ESE-1 through interaction with p300, CREB-binding protein, and Ku 70/86. J Biol Chem. 2004;279:25241-50 pubmed
    ..We have determined that Ku70, Ku86, p300, and CREB-binding protein (CBP) are ESE-1 interacting proteins...
  19. Li B, Comai L. Functional interaction between Ku and the werner syndrome protein in DNA end processing. J Biol Chem. 2000;275:28349-52 pubmed
    ..Peptide sequence analysis indicates that the two proteins are identical to Ku70 and Ku80, a heterodimer involved in double strand DNA break repair by non-homologous DNA end joining...
  20. Galande S, Kohwi Shigematsu T. Poly(ADP-ribose) polymerase and Ku autoantigen form a complex and synergistically bind to matrix attachment sequences. J Biol Chem. 1999;274:20521-8 pubmed
    ..These results provide a mechanistic link toward understanding the functional overlap of PARP and DNA-PK and suggest a novel role for these proteins in the regulation of chromatin structure and function. ..
  21. Karmakar P, Snowden C, Ramsden D, Bohr V. Ku heterodimer binds to both ends of the Werner protein and functional interaction occurs at the Werner N-terminus. Nucleic Acids Res. 2002;30:3583-91 pubmed
    ..We have now mapped this interaction physically and functionally. The Ku70 subunit specifically interacts with the N-terminus (amino acids 1-368) of WRN, while the Ku80 subunit interacts ..
  22. Ayene I, Ford L, Koch C. Ku protein targeting by Ku70 small interfering RNA enhances human cancer cell response to topoisomerase II inhibitor and gamma radiation. Mol Cancer Ther. 2005;4:529-36 pubmed
    Ku protein is a heterodimer (Ku70 and Ku86) known to play an important role in V(D)J recombination, apoptosis, telomere fusion, and double-strand break repair...
  23. Amsel A, Rathaus M, Kronman N, Cohen H. Regulation of the proapoptotic factor Bax by Ku70-dependent deubiquitylation. Proc Natl Acad Sci U S A. 2008;105:5117-22 pubmed publisher
    The DNA end-joining protein Ku70 is one of several proteins that inhibit apoptosis by sequestering the proapoptotic factor Bax from the mitochondria...
  24. Singleton B, Torres Arzayus M, Rottinghaus S, Taccioli G, Jeggo P. The C terminus of Ku80 activates the DNA-dependent protein kinase catalytic subunit. Mol Cell Biol. 1999;19:3267-77 pubmed
    ..This residue is conserved among species and lies outside of the previously reported Ku70-Ku80 interaction domain...
  25. Davis A, Lee K, Chen D. The N-terminal region of the DNA-dependent protein kinase catalytic subunit is required for its DNA double-stranded break-mediated activation. J Biol Chem. 2013;288:7037-46 pubmed publisher
    ..DNA-PK is a holoenzyme consisting of a DNA-binding (Ku70/Ku80) and catalytic (DNA-PKcs) subunit...
  26. Cohen H, Lavu S, Bitterman K, Hekking B, Imahiyerobo T, Miller C, et al. Acetylation of the C terminus of Ku70 by CBP and PCAF controls Bax-mediated apoptosis. Mol Cell. 2004;13:627-38 pubmed
    ..The Ku70 DNA end-joining protein has recently been shown to suppress apoptosis by sequestering Bax from mitochondria...
  27. Mischo H, Hemmerich P, Grosse F, Zhang S. Actinomycin D induces histone gamma-H2AX foci and complex formation of gamma-H2AX with Ku70 and nuclear DNA helicase II. J Biol Chem. 2005;280:9586-94 pubmed
    ..5 microg/ml DNA intercalating agent actinomycin D. In untreated cells, gamma-H2AX co-immunoprecipitated with Ku70, a subunit of DNA-dependent protein kinase, as well as with nuclear DNA helicase II (NDH II), a DEXH family ..
  28. Chan D, Ye R, Veillette C, Lees Miller S. DNA-dependent protein kinase phosphorylation sites in Ku 70/80 heterodimer. Biochemistry. 1999;38:1819-28 pubmed
    Ku antigen is composed of 70 and 82 kDa subunits (Ku70 and Ku80, respectively) that together bind with high affinity to ends of double-stranded DNA and other DNA structures in vitro...
  29. Cooper M, Machwe A, Orren D, Brosh R, Ramsden D, Bohr V. Ku complex interacts with and stimulates the Werner protein. Genes Dev. 2000;14:907-12 pubmed
    ..Here we identify Ku70 and Ku86 as proteins that interact with WRNp...
  30. Kelavkar U, Wang S, Badr K. Ku autoantigen (DNA helicase) is required for interleukins-13/-4-induction of 15-lipoxygenase-1 gene expression in human epithelial cells. Genes Immun. 2000;1:237-50 pubmed
    ..proteins, identified one as the 86 kDA Lupus KU autoantigen protein P86 and the second as the 70 kDa Lupus KU autoantigen protein P70. Gel shift and supershift experiments using monoclonal antibodies toward Ku antigen and its ..
  31. DeFazio L, Stansel R, Griffith J, Chu G. Synapsis of DNA ends by DNA-dependent protein kinase. EMBO J. 2002;21:3192-200 pubmed
    ..Our results suggest that DNA-PK(CS) brings DNA ends together and then undergoes activation of its kinase, presumably to regulate subsequent steps for processing and ligation of the ends. ..
  32. Ting N, Yu Y, Pohorelic B, Lees Miller S, Beattie T. Human Ku70/80 interacts directly with hTR, the RNA component of human telomerase. Nucleic Acids Res. 2005;33:2090-8 pubmed
    ..there is no sequence similarity between TLC1 and the RNA component (hTR) of human telomerase, we show that human Ku70/80 interacts with hTR both in vitro and in a cellular context...
  33. Featherstone C, Jackson S. Ku, a DNA repair protein with multiple cellular functions?. Mutat Res. 1999;434:3-15 pubmed
    ..Ku also recruits DNA-PKcs to the DSB, activating its kinase function. Targeted disruption of the genes encoding Ku70 and Ku80 has identified significant differences between Ku-deficient mice and DNA-PKcs-deficient mice...
  34. Park E, Chan D, Park J, Oettinger M, Kwon J. DNA-PK is activated by nucleosomes and phosphorylates H2AX within the nucleosomes in an acetylation-dependent manner. Nucleic Acids Res. 2003;31:6819-27 pubmed
  35. Daniel R, Katz R, Skalka A. A role for DNA-PK in retroviral DNA integration. Science. 1999;284:644-7 pubmed
    ..These results suggest that the initial events in retroviral integration are detected as DNA damage by the host cell and that completion of the integration process requires the DNA-PK-mediated repair pathway. ..
  36. Nolens G, Pignon J, Koopmansch B, ElMoualij B, Zorzi W, De Pauw E, et al. Ku proteins interact with activator protein-2 transcription factors and contribute to ERBB2 overexpression in breast cancer cell lines. Breast Cancer Res. 2009;11:R83 pubmed publisher
    ..Among the proteins retained specifically on GST-AP2 coated beads Ku70 and Ku80 proteins were identified by mass spectrometry...
  37. Yin H, Glass J. In prostate cancer cells the interaction of C/EBPalpha with Ku70, Ku80, and poly(ADP-ribose) polymerase-1 increases sensitivity to DNA damage. J Biol Chem. 2006;281:11496-505 pubmed
    ..b>Ku70, Ku80, and poly(ADP-ribose) polymerase-1 (PARP-1) were identified as proteins that associated with C/EBPalpha...
  38. Tsai Y, Bau D, Chiang C, Cheng Y, Tseng S, Tsai F. Pterygium and genetic polymorphism of DNA double strand break repair gene Ku70. Mol Vis. 2007;13:1436-40 pubmed
    ..In humans, NHEJ is the predominant repair system and Ku70 protein plays an initial and important role in the NHEJ system...
  39. Idogawa M, Masutani M, Shitashige M, Honda K, Tokino T, Shinomura Y, et al. Ku70 and poly(ADP-ribose) polymerase-1 competitively regulate beta-catenin and T-cell factor-4-mediated gene transactivation: possible linkage of DNA damage recognition and Wnt signaling. Cancer Res. 2007;67:911-8 pubmed
    ..Using immunoprecipitation and mass spectrometry, we found that two Ku proteins, Ku70 and Ku80, were also associated with the complex...
  40. Bau D, Tseng H, Wang C, Chiu C, Hua C, Wu C, et al. Oral cancer and genetic polymorphism of DNA double strand break gene Ku70 in Taiwan. Oral Oncol. 2008;44:1047-51 pubmed publisher
    The DNA repair gene Ku70, an important caretaker of the overall genome stability, is thought to play a major role in the DNA double strand break repair system...
  41. Sundaresan N, Samant S, Pillai V, Rajamohan S, Gupta M. SIRT3 is a stress-responsive deacetylase in cardiomyocytes that protects cells from stress-mediated cell death by deacetylation of Ku70. Mol Cell Biol. 2008;28:6384-401 pubmed publisher
    ..We also identified Ku70 as a new target of SIRT3...
  42. Grote J, König S, Ackermann D, Sopalla C, Benedyk M, Los M, et al. Identification of poly(ADP-ribose)polymerase-1 and Ku70/Ku80 as transcriptional regulators of S100A9 gene expression. BMC Mol Biol. 2006;7:48 pubmed
    ..Using the MRE motif for a pull-down assay, poly(ADP-ribose)polymerase-1 (PARP-1) and the heterodimeric complex Ku70/Ku80 were identified by mass spectrometry and confirmed by chromatin immunoprecipitation...
  43. Lebrun P, Montminy M, Van Obberghen E. Regulation of the pancreatic duodenal homeobox-1 protein by DNA-dependent protein kinase. J Biol Chem. 2005;280:38203-10 pubmed
    ..We identified new proteins associated with PDX-1 by mass spectrometry. These proteins, Ku70 and Ku80, are regulatory subunits of DNA-dependent protein kinase (DNA-PK)...
  44. Bianchi A, de Lange T. Ku binds telomeric DNA in vitro. J Biol Chem. 1999;274:21223-7 pubmed
    ..Our results indicate that the DNA binding characteristics of Ku are consistent with its direct interaction with telomeric DNA in mammalian cells and its proposed role as a telomere end factor. ..
  45. Chai W, Ford L, Lenertz L, Wright W, Shay J. Human Ku70/80 associates physically with telomerase through interaction with hTERT. J Biol Chem. 2002;277:47242-7 pubmed
    ..The DNA repair protein Ku (a heterodimer of Ku70 and Ku80 subunits) associates with mammalian telomeres and contributes to telomere maintenance...
  46. Sucharov C, Helmke S, Langer S, Perryman M, Bristow M, Leinwand L. The Ku protein complex interacts with YY1, is up-regulated in human heart failure, and represses alpha myosin heavy-chain gene expression. Mol Cell Biol. 2004;24:8705-15 pubmed
    ..Through peptide mass spectrometry, we identified this binding activity to be a heterodimer of Ku70 and Ku80. Expression of Ku represses the human alphaMyHC promoter in neonatal rat ventricular myocytes...
  47. Ju B, Lunyak V, Perissi V, Garcia Bassets I, Rose D, Glass C, et al. A topoisomerase IIbeta-mediated dsDNA break required for regulated transcription. Science. 2006;312:1798-802 pubmed
    ..Our data mechanistically link DNA topoisomerase IIbeta-dependent dsDNA breaks and the components of the DNA damage and repair machinery in regulated gene transcription. ..
  48. Fnu S, Williamson E, De Haro L, BRENNEMAN M, Wray J, Shaheen M, et al. Methylation of histone H3 lysine 36 enhances DNA repair by nonhomologous end-joining. Proc Natl Acad Sci U S A. 2011;108:540-5 pubmed publisher
    ..This dimethylation of H3K36 improved the association of early DNA repair components, including NBS1 and Ku70, with the induced DSB, and enhanced DSB repair...
  49. Sibani S, Price G, Zannis Hadjopoulos M. Ku80 binds to human replication origins prior to the assembly of the ORC complex. Biochemistry. 2005;44:7885-96 pubmed
    ..Interestingly, the association of Ku80 with these origins was not affected in this hypomorphic cell line, indicating that Ku and HsOrc-2 bind to origins independently of each other. ..
  50. Heo K, Kim H, Choi S, Choi J, Kim K, Gu J, et al. FACT-mediated exchange of histone variant H2AX regulated by phosphorylation of H2AX and ADP-ribosylation of Spt16. Mol Cell. 2008;30:86-97 pubmed publisher
    ..Thus, these data establish FACT as the major regulator involved in H2AX exchange process that is modulated by H2AX phosphorylation and Spt16 ADP-ribosylation. ..
  51. Sun J, Lee K, Davis A, Chen D. Human Ku70/80 protein blocks exonuclease 1-mediated DNA resection in the presence of human Mre11 or Mre11/Rad50 protein complex. J Biol Chem. 2012;287:4936-45 pubmed publisher
    ..MR), we established an in vitro DNA end resection system using a forked model dsDNA substrate and purified human Ku70/80, Mre11, Mre11/Rad50, and exonuclease 1 (Exo1)...
  52. Spagnolo L, Rivera Calzada A, Pearl L, Llorca O. Three-dimensional structure of the human DNA-PKcs/Ku70/Ku80 complex assembled on DNA and its implications for DNA DSB repair. Mol Cell. 2006;22:511-9 pubmed
    ..DNA-PKcs is recruited to DSBs by the Ku70/Ku80 heterodimer, with which it forms the core of a multiprotein complex that promotes synapsis of the broken DNA ..
  53. Ilina E, Lavrik O, Khodyreva S. Ku antigen interacts with abasic sites. Biochim Biophys Acta. 2008;1784:1777-85 pubmed publisher
    ..Ku antigen as a part of DNA-PK was shown to inhibit AP site cleavage by apurinic/apyrimidinic endonuclease 1. ..
  54. Li B, Reddy S, Comai L. Depletion of Ku70/80 reduces the levels of extrachromosomal telomeric circles and inhibits proliferation of ALT cells. Aging (Albany NY). 2011;3:395-406 pubmed
    ..Here we show that shRNA-mediated knockdown of the Ku70/80 heterodimer, a factor with functions at both pathological and natural DNA ends, inhibits ALT cell growth and ..
  55. Zhang Z, Zhu L, Lin D, Chen F, Chen D, Chen Y. The three-dimensional structure of the C-terminal DNA-binding domain of human Ku70. J Biol Chem. 2001;276:38231-6 pubmed
    The proteins Ku70 (69.8 kDa) and Ku80 (82.7 kDa) form a heterodimeric complex that is an essential component of the nonhomologous end joining DNA double-strand break repair pathway in mammalian cells...
  56. Kim E, Li K, Lieu C, Tong S, Kawai S, Fukutomi T, et al. Expression of apolipoprotein C-IV is regulated by Ku antigen/peroxisome proliferator-activated receptor gamma complex and correlates with liver steatosis. J Hepatol. 2008;49:787-98 pubmed publisher
    ..We defined a 163-bp ApoC-IV promoter as a core protein responsive element, and identified Ku antigen complex (Ku70 and Ku80) as well as nuclear receptors PPARgamma/RXRalpha as key regulators of ApoC-IV gene expression...
  57. Cohen H, Miller C, Bitterman K, Wall N, Hekking B, Kessler B, et al. Calorie restriction promotes mammalian cell survival by inducing the SIRT1 deacetylase. Science. 2004;305:390-2 pubmed
    ..SIRT1 deacetylates the DNA repair factor Ku70, causing it to sequester the proapoptotic factor Bax away from mitochondria, thereby inhibiting stress-induced ..
  58. Gu Y, Jin S, Gao Y, Weaver D, Alt F. Ku70-deficient embryonic stem cells have increased ionizing radiosensitivity, defective DNA end-binding activity, and inability to support V(D)J recombination. Proc Natl Acad Sci U S A. 1997;94:8076-81 pubmed
    ..The Ku70 protein is an additional component of DNA-PK; Ku70 forms a heterodimer with Ku80 to generate the DNA end-binding ..
  59. Walker J, Corpina R, Goldberg J. Structure of the Ku heterodimer bound to DNA and its implications for double-strand break repair. Nature. 2001;412:607-14 pubmed
    The Ku heterodimer (Ku70 and Ku80 subunits) contributes to genomic integrity through its ability to bind DNA double-strand breaks and facilitate repair by the non-homologous end-joining pathway...
  60. Jin S, Weaver D. Double-strand break repair by Ku70 requires heterodimerization with Ku80 and DNA binding functions. EMBO J. 1997;16:6874-85 pubmed
    Heterodimers of the 70 and 80 kDa Ku autoantigens (Ku70 and Ku80) activate the DNA-dependent protein kinase (DNA-PK)...