Gene Symbol: KIF3B
Description: kinesin family member 3B
Alias: FLA8, HH0048, KLP-11, kinesin-like protein KIF3B, microtubule plus end-directed kinesin motor 3B
Species: human
Products:     KIF3B

Top Publications

  1. Whitehead J, Wang S, Bost Usinger L, Hoang E, Frazer K, Burnside B. Photoreceptor localization of the KIF3A and KIF3B subunits of the heterotrimeric microtubule motor kinesin II in vertebrate retina. Exp Eye Res. 1999;69:491-503 pubmed
    ..We also describe the localization of both KIF3A and KIF3B kinesin II subunits in Xenopus retina...
  2. Jimbo T, Kawasaki Y, Koyama R, Sato R, Takada S, Haraguchi K, et al. Identification of a link between the tumour suppressor APC and the kinesin superfamily. Nat Cell Biol. 2002;4:323-7 pubmed
    ..We show here that APC interacts with the kinesin superfamily (KIF) 3A-KIF3B proteins, microtubule plus-end-directed motor proteins, through an association with the kinesin superfamily-..
  3. Duangtum N, Junking M, Sawasdee N, Cheunsuchon B, Limjindaporn T, Yenchitsomanus P. Human kidney anion exchanger 1 interacts with kinesin family member 3B (KIF3B). Biochem Biophys Res Commun. 2011;413:69-74 pubmed publisher
    ..We report here that kAE1 interacts with kinesin family member 3B (KIF3B) in kidney cells and a dileucine motif at the carboxyl terminus of kAE1 contributes to this interaction...
  4. Wu Y, Dai X, Li Q, Chen C, Mai W, Hussain Z, et al. Kinesin-2 mediates physical and functional interactions between polycystin-2 and fibrocystin. Hum Mol Genet. 2006;15:3280-92 pubmed
    ..Here, we demonstrated, by yeast two-hybrid and biochemical assays, that KIF3B, a motor subunit of kinesin-2, associates with PC2 and FPC...
  5. Nonaka S, Tanaka Y, Okada Y, Takeda S, Harada A, Kanai Y, et al. Randomization of left-right asymmetry due to loss of nodal cilia generating leftward flow of extraembryonic fluid in mice lacking KIF3B motor protein. Cell. 1998;95:829-37 pubmed
    Microtubule-dependent motor, murine KIF3B, was disrupted by gene targeting. The null mutants did not survive beyond midgestation, exhibiting growth retardation, pericardial sac ballooning, and neural tube disorganization...
  6. Haraguchi K, Hayashi T, Jimbo T, Yamamoto T, Akiyama T. Role of the kinesin-2 family protein, KIF3, during mitosis. J Biol Chem. 2006;281:4094-9 pubmed
    ..Furthermore, we found that expression of a mutant KIF3B, which is able to associate with KIF3A but not KAP3 in NIH3T3 cells, caused chromosomal aneuploidy and abnormal ..
  7. Yamazaki H, Nakata T, Okada Y, Hirokawa N. KIF3A/B: a heterodimeric kinesin superfamily protein that works as a microtubule plus end-directed motor for membrane organelle transport. J Cell Biol. 1995;130:1387-99 pubmed
    We cloned a new member of the murine brain kinesin superfamily, KIF3B, and found that its amino acid sequence is highly homologous but not identical to KIF3A, which we previously cloned and named KIF3 (47% identical)...
  8. Keil R, Kiessling C, Hatzfeld M. Targeting of p0071 to the midbody depends on KIF3. J Cell Sci. 2009;122:1174-83 pubmed publisher
    ..p0071 interacted with the kinesin-II family member KIF3b, and knockdown of KIF3b interfered with p0071 midbody recruitment whereas Ect2 or RhoA localization was not ..
  9. Imamura T, Huang J, Usui I, Satoh H, Bever J, Olefsky J. Insulin-induced GLUT4 translocation involves protein kinase C-lambda-mediated functional coupling between Rab4 and the motor protein kinesin. Mol Cell Biol. 2003;23:4892-900 pubmed

More Information


  1. Engelke M, Waas B, Kearns S, Suber A, Boss A, Allen B, et al. Acute Inhibition of Heterotrimeric Kinesin-2 Function Reveals Mechanisms of Intraflagellar Transport in Mammalian Cilia. Curr Biol. 2019;: pubmed publisher
    ..Loss of function in any subunit of the heterotrimeric KIF3A/KIF3B/KAP kinesin-2 motor prevents ciliogenesis in mammalian cells and has hindered an understanding of how kinesin-2 ..
  2. Schimert K, Budaitis B, Reinemann D, Lang M, Verhey K. Intracellular cargo transport by single-headed kinesin motors. Proc Natl Acad Sci U S A. 2019;116:6152-6161 pubmed publisher
    ..Strikingly, monomeric versions of the kinesin-2 family motors KIF3A and KIF3B are able to drive Golgi dispersion in cells, and teams of monomeric KIF3B motors can generate over 8 pN of force ..
  3. Ji L, Zhu Z, He C, Shen X. MiR-127-3p targets KIF3B to inhibit the development of oral squamous cell carcinoma. Eur Rev Med Pharmacol Sci. 2019;23:630-640 pubmed publisher
    ..MiR-127-3p and KIF3B expressions were observed via quantitative Real-time polymerase chain reaction (qRT-PCR) or Western blot in OSCC...
  4. Nag S, Rani S, Mahanty S, Bissig C, Arora P, Azevedo C, et al. Rab4A organizes endosomal domains for sorting cargo to lysosome-related organelles. J Cell Sci. 2018;131: pubmed publisher
    ..Consistent with this, inactivation of rabenosyn-5, KIF3A or KIF3B phenocopied the defects observed in Rab4A-knockdown melanocytes...
  5. Quinn S, Howsmon D, Hahn J, Gilbert S. Kinesin-2 heterodimerization alters entry into a processive run along the microtubule but not stepping within the run. J Biol Chem. 2018;293:13389-13400 pubmed publisher
    ..s-1 These results led us to hypothesize that heterodimerization of KIF3A with KIF3C and KIF3A with KIF3B altered the intrinsic catalytic properties of each motor domain...
  6. Liang Y, Zhu X, Wu Q, Pan J. Ciliary Length Sensing Regulates IFT Entry via Changes in FLA8/KIF3B Phosphorylation to Control Ciliary Assembly. Curr Biol. 2018;28:2429-2435.e3 pubmed publisher
    ..Previously, we showed that phosphorylation of the FLA8/KIF3B subunit of the anterograde kinesin-II IFT motor blocks IFT entry and flagellar assembly in Chlamydomonas [11]...
  7. Broekhuis J, Verhey K, Jansen G. Regulation of cilium length and intraflagellar transport by the RCK-kinases ICK and MOK in renal epithelial cells. PLoS ONE. 2014;9:e108470 pubmed publisher
    ..the effects of ICK and MOK on the IFT machinery, we set up live imaging of five fluorescently tagged IFT proteins: KIF3B, a subunit of kinesin-II, the main anterograde IFT motor, complex A protein IFT43, complex B protein IFT20, BBSome ..
  8. Craige B, Witman G. Flipping a phosphate switch on kinesin-II to turn IFT around. Dev Cell. 2014;30:492-3 pubmed publisher
    ..In this issue of Developmental Cell, Liang et al. (2014) report that IFT is regulated in part by the phosphorylation status of the kinesin-II subunit FLA8/KIF3B.
  9. Albracht C, Rank K, Obrzut S, Rayment I, Gilbert S. Kinesin-2 KIF3AB exhibits novel ATPase characteristics. J Biol Chem. 2014;289:27836-48 pubmed publisher
    ..To define the ATPase mechanism and coordination of KIF3A and KIF3B stepping, a presteady-state kinetic analysis was pursued...
  10. Funabashi T, Katoh Y, Okazaki M, Sugawa M, Nakayama K. Interaction of heterotrimeric kinesin-II with IFT-B-connecting tetramer is crucial for ciliogenesis. J Cell Biol. 2018;217:2867-2876 pubmed publisher
    ..assay, we identified in this study a three-to-four protein interaction involving the kinesin-II trimer KIF3A-KIF3B-KAP3 and the IFT-B-connecting tetramer IFT38-IFT52-IFT57-IFT88; among the kinesin-II subunits, KIF3B contributed ..
  11. Liang Y, Pang Y, Wu Q, Hu Z, Han X, Xu Y, et al. FLA8/KIF3B phosphorylation regulates kinesin-II interaction with IFT-B to control IFT entry and turnaround. Dev Cell. 2014;30:585-97 pubmed publisher
    ..We show that the kinesin-II motor subunit FLA8, a homolog of KIF3B, is phosphorylated on the conserved S663 by a calcium-dependent kinase in Chlamydomonas...
  12. Ginkel L, Wordeman L. Expression and partial characterization of kinesin-related proteins in differentiating and adult skeletal muscle. Mol Biol Cell. 2000;11:4143-58 pubmed
    ..pan-kinesin antibodies to screen a differentiating C2C12 cell library, we identified the kinesin proteins KIF3A, KIF3B, and conventional kinesin heavy chain to be present in differentiating skeletal muscle...
  13. Kaur M, Panikkath D, Yan X, Liu Z, Berel D, Li D, et al. Perianal Crohn's Disease is Associated with Distal Colonic Disease, Stricturing Disease Behavior, IBD-Associated Serologies and Genetic Variation in the JAK-STAT Pathway. Inflamm Bowel Dis. 2016;22:862-9 pubmed publisher
    ..008) antibody levels. pCD was associated with known inflammatory bowel disease loci, including KIF3B, CRTC3, TRAF3IP2, JAZF1, NRIP1, MST1, FUT2, and PTGER (all P < 0.05)...
  14. Zhao Y, Mu D, Wang D, Han Y, Hou C, Zhu J. Analysis of the function of KIF3A and KIF3B in the spermatogenesis in Boleophthalmus pectinirostris. Fish Physiol Biochem. 2018;44:769-788 pubmed publisher
    ..and maintenance of the flagella and intracellular transport of membrane-bound organelles required KIF3A and KIF3B. Our main goal was to test KIF3A and KIF3B location during spermatogenesis of Boleophthalmus pectinirostris...
  15. Zhou W, Yuan T, Gao Y, Yin P, Liu W, Pan C, et al. IL-1β-induces NF-κB and upregulates microRNA-372 to inhibit spinal cord injury recovery. J Neurophysiol. 2017;117:2282-2291 pubmed publisher
    ..In this study we have shown that in cultured human neural stem cells (hNSC), IL-1β reduced the expression of both KIF3B (kinesin family member 3B) and NOSIP (nitric oxide synthase-interacting protein), two key modulators for ..
  16. Nagata K, Puls A, Futter C, Aspenstrom P, Schaefer E, Nakata T, et al. The MAP kinase kinase kinase MLK2 co-localizes with activated JNK along microtubules and associates with kinesin superfamily motor KIF3. EMBO J. 1998;17:149-58 pubmed
  17. Reed A, Loh N, Terryn S, Lippiat J, Partridge C, Galvanovskis J, et al. CLC-5 and KIF3B interact to facilitate CLC-5 plasma membrane expression, endocytosis, and microtubular transport: relevance to pathophysiology of Dent's disease. Am J Physiol Renal Physiol. 2010;298:F365-80 pubmed publisher
    ..Here, we report that CLC-5 interacts with kinesin family member 3B (KIF3B), a heterotrimeric motor protein that facilitates fast anterograde translocation of membranous organelles...
  18. Shen H, Xiao Y, She Z, Tan F, Yang W. A novel role of KIF3b in the seminoma cell cycle. Exp Cell Res. 2017;352:95-103 pubmed publisher
    b>KIF3b is a protein of the kinesin-2 family which plays an important role in intraflagellar transport. Testis cancer is a common cancer among young men. Its diagnostic rate is increasing and over half of the cases are seminomas...
  19. Becher A, Eiseler T, Porzner M, Walther P, Keil R, Bobrovich S, et al. The armadillo protein p0071 controls KIF3 motor transport. J Cell Sci. 2017;130:3374-3387 pubmed publisher
    ..p0071 directly interacts with the KIF3 motor subunit KIF3B. Our data indicate that p0071 also interacts with the kinesin cargo adaptor protein KAP3 (also known as KIFAP3) ..
  20. Feng D, Chen Z, Yang K, Miao S, Xu B, Kang Y, et al. The cytoplasmic tail of rhodopsin triggers rapid rod degeneration in kinesin-2 mutants. J Biol Chem. 2017;292:17375-17386 pubmed publisher
    ..Here we examined photoreceptor development in zebrafish kif3a and kif3b mutants, which affect two subunits of the kinesin-2 complex...
  21. Ferreira P, Yunfei C, Schick D, Roepman R. The cyclophilin-like domain mediates the association of Ran-binding protein 2 with subunits of the 19 S regulatory complex of the proteasome. J Biol Chem. 1998;273:24676-82 pubmed
  22. Lolkema M, Mans D, Snijckers C, van Noort M, van Beest M, Voest E, et al. The von Hippel-Lindau tumour suppressor interacts with microtubules through kinesin-2. FEBS Lett. 2007;581:4571-6 pubmed
    ..The interaction with kinesin-2 facilitates pVHL binding to microtubules. These data suggest that microtubule-dependent functions of pVHL are influenced by kinesin-2. ..
  23. Oh D, Houston D. Role of maternal Xenopus syntabulin in germ plasm aggregation and primordial germ cell specification. Dev Biol. 2017;432:237-247 pubmed publisher
    ..Additionally, we show that a localized kinesin-like protein, Kif3b, is also required for germ plasm aggregation and that Sybu functionally interacts with Kif3b and Kif4a in germ ..
  24. Baker S, Freeman K, Luby Phelps K, Pazour G, Besharse J. IFT20 links kinesin II with a mammalian intraflagellar transport complex that is conserved in motile flagella and sensory cilia. J Biol Chem. 2003;278:34211-8 pubmed
    ..IFT20 exhibited a strong interaction with IFT57/Hippi and the kinesin II subunit, KIF3B. Our data indicate that all four mammalian IFT proteins are part of a highly conserved complex in multiple ..
  25. Araki N, Ishigami T, Ushio H, Minegishi S, Umemura M, Miyagi Y, et al. Identification of NPC2 protein as interaction molecule with C2 domain of human Nedd4L. Biochem Biophys Res Commun. 2009;388:290-6 pubmed publisher
    ..NPC2 might regulate sodium reabsorption in the terminal nephron by interacting with ENaC-Nedd4L system. ..
  26. Zhang Y, Hancock W. The two motor domains of KIF3A/B coordinate for processive motility and move at different speeds. Biophys J. 2004;87:1795-804 pubmed
    ..To test this, we investigated the motility of wild-type KIF3A/B heterodimer and chimaeric KIF3A/A and KIF3B/B homodimers made by splicing the head of one subunit to the rod and tail of the other...
  27. Schonteich E, Wilson G, Burden J, Hopkins C, Anderson K, Goldenring J, et al. The Rip11/Rab11-FIP5 and kinesin II complex regulates endocytic protein recycling. J Cell Sci. 2008;121:3824-33 pubmed publisher
    ..Thus, we propose that the Rip11/FIP5-kinesin-II complex has a key role in the routing of internalized receptors through the perinuclear recycling endosomes. ..
  28. Zhao Y, Yang H, Zhang D, Han Y, Hou C, Zhu J. Dynamic transcription and expression patterns of KIF3A and KIF3B genes during spermiogenesis in the shrimp, Palaemon carincauda. Anim Reprod Sci. 2017;184:59-77 pubmed publisher
    ..The microtubule-based motor proteins KIF3A and KIF3B are required for the progression of the stages of spermiogenesis...
  29. Carpenter B, Barry R, Verhey K, Allen B. The heterotrimeric kinesin-2 complex interacts with and regulates GLI protein function. J Cell Sci. 2015;128:1034-50 pubmed publisher
    ..To examine a role for the heterotrimeric KIF3A-KIF3B-KAP3 kinesin-2 motor complex in regulating GLI activity, we performed a series of structure-function analyses ..
  30. Huang X, Liu F, Zhu C, Cai J, Wang H, Wang X, et al. Suppression of KIF3B expression inhibits human hepatocellular carcinoma proliferation. Dig Dis Sci. 2014;59:795-806 pubmed publisher
    ..g. mitosis, motility and organelle transportation) and have been involved in human carcinogenesis. KIF3B, the kinesin superfamily of proteins (KIFs), plays an important role in the regulation of mitotic progression...
  31. Shimizu K, Shirataki H, Honda T, Minami S, Takai Y. Complex formation of SMAP/KAP3, a KIF3A/B ATPase motor-associated protein, with a human chromosome-associated polypeptide. J Biol Chem. 1998;273:6591-4 pubmed
    ..indicated that HCAP was ubiquitously expressed and highly concentrated in the nuclear fraction, where SMAP and KIF3B were also present...
  32. Navarro E, Espinosa L, Adell T, Tora M, Berrozpe G, Real F. Expressed sequence tag (EST) phenotyping of HT-29 cells: cloning of ser/thr protein kinase EMK1, kinesin KIF3B, and of transcripts that include Alu repeated elements. Biochim Biophys Acta. 1999;1450:254-64 pubmed well as unknown transcripts (three clones), and the human homologues of the molecular motor kinesin KIF3B and of the ser/thr protein kinase EMK1...
  33. Aoki T, Ueda S, Kataoka T, Satoh T. Regulation of mitotic spindle formation by the RhoA guanine nucleotide exchange factor ARHGEF10. BMC Cell Biol. 2009;10:56 pubmed publisher
    ..We further isolated the kinesin-like motor protein KIF3B as a binding partner of ARHGEF10. Knockdown of KIF3B again caused multipolar spindle phenotypes...