keratin 12

Summary

Gene Symbol: keratin 12
Description: keratin 12
Alias: K12, keratin, type I cytoskeletal 12, CK-12, cytokeratin-12, keratin 12, type I
Species: human
Products:     keratin 12

Top Publications

  1. Nielsen K, Orntoft T, Hjortdal J, Rasmussen T, Ehlers N. A novel mutation as the basis for asymptomatic meesmann dystrophy in a Danish family. Cornea. 2008;27:100-2 pubmed publisher
    ..Blood samples were collected. DNA from the leukocyte population was isolated, and the cytokeratin 12 (KRT12) gene was partially sequenced. This Danish family harbors a 451G-->T mutation...
  2. Irvine A, Corden L, Swensson O, Swensson B, Moore J, Frazer D, et al. Mutations in cornea-specific keratin K3 or K12 genes cause Meesmann's corneal dystrophy. Nat Genet. 1997;16:184-7 pubmed
    The intermediate filament cytoskeleton of corneal epithelial cells is composed of cornea-specific keratins K3 and K12 (refs 1,2)...
  3. Nishida K, Honma Y, Dota A, Kawasaki S, Adachi W, Nakamura T, et al. Isolation and chromosomal localization of a cornea-specific human keratin 12 gene and detection of four mutations in Meesmann corneal epithelial dystrophy. Am J Hum Genet. 1997;61:1268-75 pubmed
    b>Keratin 12 (K12) is an intermediate-filament protein expressed specifically in corneal epithelium. Recently, we isolated K12 cDNA from a human corneal epithelial cDNA library and determined its full sequence...
  4. Corden L, Swensson O, Swensson B, Smith F, Rochels R, Uitto J, et al. Molecular genetics of Meesmann's corneal dystrophy: ancestral and novel mutations in keratin 12 (K12) and complete sequence of the human KRT12 gene. Exp Eye Res. 2000;70:41-9 pubmed
    Recently, we identified the first mutations in corneal keratins K3 and K12 in families with Meesmann's corneal dystrophy (MCD)...
  5. Corden L, Swensson O, Swensson B, Rochels R, Wannke B, Thiel H, et al. A novel keratin 12 mutation in a German kindred with Meesmann's corneal dystrophy. Br J Ophthalmol. 2000;84:527-30 pubmed
    ..study a kindred with Meesmann's corneal dystrophy (MCD) to determine if a mutation within the cornea specific K3 or K12 genes is responsible for the disease phenotype...
  6. Takahashi K, Takahashi K, Murakami A, Okisaka S, Kimura T, Kanai A. Heterozygous Ala137Pro mutation in keratin 12 gene found in Japanese with Meesmann's corneal dystrophy. Jpn J Ophthalmol. 2002;46:673-4 pubmed
    ..Sequencing of the keratin 3 and keratin 12 genes was performed in 2 patients who were siblings and in an unaffected individual in the same family...
  7. Yoon M, Warren J, Holsclaw D, Gritz D, Margolis T. A novel arginine substitution mutation in 1A domain and a novel 27 bp insertion mutation in 2B domain of keratin 12 gene associated with Meesmann's corneal dystrophy. Br J Ophthalmol. 2004;88:752-6 pubmed
    ..To determine the disease causing gene defects in two patients with Meesmann's corneal dystrophy...
  8. Chen Y, Tseng S, Chao S. Novel mutations in the helix termination motif of keratin 3 and keratin 12 in 2 Taiwanese families with Meesmann corneal dystrophy. Cornea. 2005;24:928-32 pubmed
    To analyze mutations of the keratin 3 gene (KRT3) and keratin 12 gene (KRT12) in 2 Taiwanese families with Meesmann corneal dystrophy (MCD)...
  9. Nichini O, Manzi V, Munier F, Schorderet D. Meesmann corneal dystrophy (MECD): report of 2 families and a novel mutation in the cornea specific keratin 12 (KRT12) gene. Ophthalmic Genet. 2005;26:169-73 pubmed
    ..In one family, the M129T heterozygous mutation was observed in KRT12. In the second family, we identified a novel I426S heterozygous mutation in exon 6 of KRT12. ..

More Information

Publications120 found, 100 shown here

  1. Sullivan L, Baylin E, Font R, Daiger S, Pepose J, Clinch T, et al. A novel mutation of the Keratin 12 gene responsible for a severe phenotype of Meesmann's corneal dystrophy. Mol Vis. 2007;13:975-80 pubmed
    To determine if a mutation within the coding region of the keratin 12 gene (KRT12) is responsible for a severe form of Meesmann's corneal dystrophy...
  2. Seto T, Fujiki K, Kishishita H, Fujimaki T, Murakami A, Kanai A. A novel mutation in the cornea-specific keratin 12 gene in Meesmann corneal dystrophy. Jpn J Ophthalmol. 2008;52:224-226 pubmed publisher
    To report a novel mutation in the keratin 12 gene (KRT12) found in a Japanese family in association with Meesmann corneal dystrophy (MECD)...
  3. Liimatta K, Flaherty E, Ro G, Nguyen D, Prado C, Purdy A. A Putative Acetylation System in Vibrio cholerae Modulates Virulence in Arthropod Hosts. Appl Environ Microbiol. 2018;84: pubmed publisher
    ..These findings further highlight the many layers of regulation that tune bacterial metabolism to alter the trajectory of interactions between bacteria and their hosts. ..
  4. Liu D, Torchen L, Sung Y, Paparodis R, Legro R, Grebe S, et al. Evidence for gonadotrophin secretory and steroidogenic abnormalities in brothers of women with polycystic ovary syndrome. Hum Reprod. 2014;29:2764-72 pubmed publisher
    ..This research was supported by P50 HD044405 (A.D.), K12 HD055884 (L.C.T.), U54 HD034449 (A.D., R.S.L.) from the National Institute of Child Health and Development...
  5. Lu X, Lu H, Guo L, Zhang Z, Zhao X, Zhong M, et al. Cloning and characterization of a novel hemocyanin variant LvHMCV4 from shrimp Litopenaeus vannamei. Fish Shellfish Immunol. 2015;46:398-405 pubmed publisher
    ..Infection by Escherichia coli K12, Vibrio parahaemolyticus, Vibrio alginolyticus, Vibrio fluvialis, Streptococcus pyogenes or Staphylococcus aureus ..
  6. Delp E, Swamynathan S, Kao W, Swamynathan S. Spatiotemporally Regulated Ablation of Klf4 in Adult Mouse Corneal Epithelial Cells Results in Altered Epithelial Cell Identity and Disrupted Homeostasis. Invest Ophthalmol Vis Sci. 2015;56:3549-58 pubmed publisher
  7. Singh N, Bezdan D, Checinska Sielaff A, Wheeler K, Mason C, Venkateswaran K. Multi-drug resistant Enterobacter bugandensis species isolated from the International Space Station and comparative genomic analyses with human pathogenic strains. BMC Microbiol. 2018;18:175 pubmed publisher
    ..more than 60 genes, including upregulation of drug efflux systems that have been reported in Escherichia coli K12, was also observed in the ISS strains...
  8. Tanvir R, Sajid I, Hasnain S, Kulik A, Grond S. Rare actinomycetes Nocardia caishijiensis and Pseudonocardia carboxydivorans as endophytes, their bioactivity and metabolites evaluation. Microbiol Res. 2016;185:22-35 pubmed publisher
    ..tropicalis (20 mm), S. aureus ATCC 25923 (17 mm), MRSA (17 mm), E. coli K12 (W1130) (16 mm) and Chlorella vulgaris (10 mm)...
  9. Rashid H, Rahman M. Possible transfer of plasmid mediated third generation cephalosporin resistance between Escherichia coli and Shigella sonnei in the human gut. Infect Genet Evol. 2015;30:15-8 pubmed publisher
    ..coli K12 and S. sonnei. Plasmid profile analysis revealed that the first-generation E. coli K12 and S...
  10. Arivaradarajan P, Warburton P, Paramasamy G, Nair S, Allan E, Mullany P. Identification of an antibacterial protein by functional screening of a human oral metagenomic library. FEMS Microbiol Lett. 2015;362:fnv142 pubmed publisher
    ..subflava ATCC A1078, E. coli K12 JM109 and BL21(DE3) Fusobacterium nucleatum ATCC 25586 and NCTC 11326, Prevotella intermedia ATCC 25611, ..
  11. Xu X, McAteer S, Tree J, Shaw D, Wolfson E, Beatson S, et al. Lysogeny with Shiga toxin 2-encoding bacteriophages represses type III secretion in enterohemorrhagic Escherichia coli. PLoS Pathog. 2012;8:e1002672 pubmed publisher
    ..This regulation was confirmed in an E. coli K12 background transduced with a marked Stx2 phage followed by measurement of a T3S reporter controlled by induced ..
  12. Du Z, Nandakumar R, Nickerson K, Li X. Proteomic adaptations to starvation prepare Escherichia coli for disinfection tolerance. Water Res. 2015;69:110-9 pubmed publisher
    ..The proteomes of normal and starved Escherichia coli (K12 MG1655) cells were studied using quantitative proteomics...
  13. Tudor S, Gîză D, Lin H, Fabris L, Yoshiaki K, D Abundo L, et al. Cellular and Kaposi's sarcoma-associated herpes virus microRNAs in sepsis and surgical trauma. Cell Death Dis. 2014;5:e1559 pubmed publisher
    ..groups had high KSHV miRNAs levels compared with controls; Afro-American patients had higher levels of KSHV-miR-K12-12* than non-Afro-American patients...
  14. Hayashi Takanaka Y, Maehara K, Harada A, Umehara T, Yokoyama S, Obuse C, et al. Distribution of histone H4 modifications as revealed by a panel of specific monoclonal antibodies. Chromosome Res. 2015;23:753-66 pubmed publisher
    ..generated a panel of mouse monoclonal antibodies against histone H4 modifications, including acetylation at K5, K8, K12, and K16, and different levels of methylation at K20...
  15. Ketchum C, Larsen C, McNeil A, Meyer Ficca M, Meyer R. Early Histone H4 Acetylation during Chromatin Remodeling in Equine Spermatogenesis. Biol Reprod. 2017;: pubmed publisher
    ..In the stallion, but not the mouse, H4 was already highly acetylated in lysine residues K5, K8 and K12 in round spermatids almost immediately after meiotic division...
  16. Loughner C, Tiwari A, Kenchegowda D, Swamynathan S, Swamynathan S. Spatiotemporally Controlled Ablation of Klf5 Results in Dysregulated Epithelial Homeostasis in Adult Mouse Corneas. Invest Ophthalmol Vis Sci. 2017;58:4683-4693 pubmed publisher
  17. Orru G, Demontis C, Mameli A, Tuveri E, Coni P, Pichiri G, et al. The Selective Interaction of Pistacia lentiscus Oil vs. Human Streptococci, an Old Functional Food Revisited with New Tools. Front Microbiol. 2017;8:2067 pubmed publisher
    ..Two probiotic strains of S. salivarius K12/M18 appeared to be non-sensitive to this product, while a set of five different pathogenic strains (S...
  18. Nikolić B, Vasilijević B, Mitić Ćulafić D, Vuković Gačić B, Knežević Vukćević J. Comparative study of genotoxic, antigenotoxic and cytotoxic activities of monoterpenes camphor, eucalyptol and thujone in bacteria and mammalian cells. Chem Biol Interact. 2015;242:263-71 pubmed publisher
    ..eucalyptol and thujone were determined in bacteria and mammalian cells using alkaline comet assay, Escherichia coli K12 reversion test and MTT assay, respectively...
  19. Fischer N, Sechet E, Friedman R, Amiot A, Sobhani I, Nigro G, et al. Histone deacetylase inhibition enhances antimicrobial peptide but not inflammatory cytokine expression upon bacterial challenge. Proc Natl Acad Sci U S A. 2016;113:E2993-3001 pubmed publisher
    ..Using an in vitro model of colonic epithelial cells challenged with Escherichia coli K12, we showed that inhibition of histone deacetylases (HDAC) by trichostatin A dramatically enhanced induction of HBD2 ..
  20. Hosseinkhan N, Zarrineh P, Rokni Zadeh H, Ashouri M, Masoudi Nejad A. Co-expressional conservation in virulence and stress related genes of three Gammaproteobacterial species: Escherichia coli, Salmonella enterica and Pseudomonas aeruginosa. Mol Biosyst. 2015;11:3137-48 pubmed publisher
    ..kinds of stresses in three most intensively studied members of Gammaproteobacteria including Escherichia coli K12, Pseudomonas aeruginosa PAO1 and Salmonella enterica for which large sets of gene expression data are available...
  21. Jobling M. The chromosomal nature of LT-II enterotoxins solved: a lambdoid prophage encodes both LT-II and one of two novel pertussis-toxin-like toxin family members in type II enterotoxigenic Escherichia coli. Pathog Dis. 2016;74: pubmed publisher
    ..coli K12 strains...
  22. Qian Y, Ding Q, Li Y, Zou Z, Yan B, Ou L. Phosphorylation of uridine and cytidine by uridine-cytidine kinase. J Biotechnol. 2014;188:81-7 pubmed publisher
    ..The ack and uck genes from Escherichia coli K12 and the uck1, uck2 and ack genes from Lactobacillus bulgaricus ATCC 11842 were cloned and inserted into the plasmid ..
  23. Halter M, Zahn J. Characterization of a novel lytic bacteriophage from an industrial Escherichia coli fermentation process and elimination of virulence using a heterologous CRISPR-Cas9 system. J Ind Microbiol Biotechnol. 2018;45:153-163 pubmed publisher
    ..bacteriophage, referred to as bacteriophage DTL that is capable of rapid lytic infections of an Escherichia coli K12 strain used for commercial production of 1,3-propanediol (PDO)...
  24. Douvaras P, Dorà N, Mort R, Lodge E, Hill R, West J. Abnormal corneal epithelial maintenance in mice heterozygous for the micropinna microphthalmia mutation Mp. Exp Eye Res. 2016;149:26-39 pubmed publisher
    ..formed an abnormally wide limbal vasculature ring, K19-positive cells were distributed more widely than normal and K12 was weakly expressed in the peripheral cornea...
  25. Zimmermann E, Laureano J, Dos Santos C, Schmidt S, Lagishetty C, De Castro W, et al. Simultaneous Semimechanistic Population Analyses of Levofloxacin in Plasma, Lung, and Prostate To Describe the Influence of Efflux Transporters on Drug Distribution following Intravenous and Intratracheal Administration. Antimicrob Agents Chemother. 2016;60:946-54 pubmed publisher
    ..180 liters/h. The intercompartmental distribution rate constants (K values) were as follows: K12, 8.826 h(-1); K21, 7.271 h(-1); K13, 0.047 h(-1); K31, 7.738 h(-1); K14, 0.908 h(-1); K41, 0...
  26. Chen K, Liu X, Zhao Y, Hieronymi K, Rossi G, Auvil L, et al. Long-Term Administration of Conjugated Estrogen and Bazedoxifene Decreased Murine Fecal ?-Glucuronidase Activity Without Impacting Overall Microbiome Community. Sci Rep. 2018;8:8166 pubmed publisher
    ..We further confirmed in Escherichia coli K12 and Lactobacillus gasseri ADH that Tamoxifen-, 4-hydroxy-Tamoxifen- and Estradiol-Glucuronides competed for GUS ..
  27. Montagud Romero S, Montesinos J, Pascual M, Aguilar M, Roger Sánchez C, Guerri C, et al. `Up-regulation of histone acetylation induced by social defeat mediates the conditioned rewarding effects of cocaine. Prog Neuropsychopharmacol Biol Psychiatry. 2016;70:39-48 pubmed publisher
    ..Three weeks after the last defeat, mice displayed an increase in histone H4(K12) acetylation and an upregulation of histone acetyl transferase (HAT) activity in the hippocampus...
  28. Gupte K, Vanikar A, Trivedi H, Patel C, Patel J. In-vitro generation of interleukin-10 secreting B-regulatory cells from donor adipose tissue derived mesenchymal stem cells and recipient peripheral blood mononuclear cells for potential cell therapy. Biomed J. 2017;40:49-54 pubmed publisher
    ..coli K12 strain at 37 °C with 5% CO2...
  29. Sui M, Li S, Pan Q, Sun G, Geng Y. Theoretical characterization on photoelectric properties of benzothiadiazole- and fluorene-based small molecule acceptor materials for the organic photovoltaics. J Mol Model. 2017;23:28 pubmed publisher
    ..Therefore, taking the K12/1 as a reference, we have theoretically designed a series of small molecule acceptors (2-4)...
  30. Agrawal A, Dudhedia M, Zimny E. Hot Melt Extrusion: Development of an Amorphous Solid Dispersion for an Insoluble Drug from Mini-scale to Clinical Scale. AAPS PharmSciTech. 2016;17:133-47 pubmed publisher
    ..Formulation blends of compound X with PVP K12 and PVP VA64 polymers were extruded through a 9-mm twin screw mini-extruder...
  31. Di Pierro F, Colombo M, Giuliani M, Danza M, Basile I, Bollani T, et al. Effect of administration of Streptococcus salivarius K12 on the occurrence of streptococcal pharyngo-tonsillitis, scarlet fever and acute otitis media in 3 years old children. Eur Rev Med Pharmacol Sci. 2016;20:4601-4606 pubmed
    Streptococcus salivarius K12 (BLIS K12) is a probiotic strain strongly antagonistic to the growth of Streptococcus pyogenes, the most important bacterial cause of pharyngeal infections in humans...
  32. Mao X, Ma Q, Liu B, Chen X, Zhang H, Xu Y. Revisiting operons: an analysis of the landscape of transcriptional units in E. coli. BMC Bioinformatics. 2015;16:356 pubmed publisher
    ..Here we present a computational study of the landscape of the transcriptional units (TUs) of E. coli K12, revealed by the available genomic and transcriptomic data, providing new understanding about the complexity of TUs ..
  33. Sührer I, Langemann T, Lubitz W, Weuster Botz D, Castiglione K. A novel one-step expression and immobilization method for the production of biocatalytic preparations. Microb Cell Fact. 2015;14:180 pubmed publisher
    ..expression and immobilization technique for enzymes on the example of the β-galactosidase from Escherichia coli K12. The enzyme was expressed in E...
  34. Pourseif M, Moghaddam G, Naghili B, Saeedi N, Parvizpour S, Nematollahi A, et al. A novel in silico minigene vaccine based on CD4+ T-helper and B-cell epitopes of EG95 isolates for vaccination against cystic echinococcosis. Comput Biol Chem. 2018;72:150-163 pubmed publisher
    ..It was also codon-optimized for high-level expression in Escherichia coli k12. Taken all, we propose the present in silico vaccine constructs as a promising platform for the generation of ..
  35. Leuzzi A, Grossi M, Di Martino M, Pasqua M, Micheli G, Colonna B, et al. Role of the SRRz/Rz1 lambdoid lysis cassette in the pathoadaptive evolution of Shigella. Int J Med Microbiol. 2017;307:268-275 pubmed publisher
    ..coli K12 by virtue of its beneficial effect on cell physiology, has undergone a significant decay in Shigella...
  36. Manrique Y, Suriyarak S, Gibis M, Schmidt H, Weiss J. Survival of spoilage bacteria subjected to sequential eugenol and temperature treatments. Int J Food Microbiol. 2016;218:6-16 pubmed publisher
    ..temperature on the survival of two model spoilage organisms, Staphylococcus carnosus LTH1502 and Escherichia coli K12 C600, were studied...
  37. Tokunaga H, Maeda J, Arakawa T, Tokunaga M. Reversible Activation of Halophilic ?-lactamase from Methanol-Induced Inactive Form: Contrast to Irreversible Inactivation of Non-Halophilic Counterpart. Protein J. 2017;36:228-237 pubmed publisher
    ..In contrast, non-halophilic ?-lactamase from Escherichia coli K12 strain MG1655 (EcBla) was irreversibly denatured in the presence of 40% methanol...
  38. Chojecka A, Tarka P, Kierzkowska A, Nitsch Osuch A, Kanecki K. Neutralization efficiency of alcohol based products used for rapid hand disinfection. Rocz Panstw Zakl Hig. 2017;68:389-394 pubmed
    ..of the tested products and their derivatives produced during neutralization (10 s) for test organisms (Staphylococcus aureus ATCC 6538; Pseudomonas aeruginosa ATCC 15442; Enterococcus hirae ATCC 10541; Escherichia coli K12 NCTC 10538)
  39. Lin J, Tsai Y, Liu Y, Lin Y, Tai H, Chen C. Identification of 2-oxohistidine Interacting Proteins Using E. coli Proteome Chips. Mol Cell Proteomics. 2016;15:3581-3593 pubmed
    ..coli K12 proteome microarray containing >4200 proteins...
  40. Meng F, Meckel J, Zhang F. Investigation of itraconazole ternary amorphous solid dispersions based on povidone and Carbopol. Eur J Pharm Sci. 2017;106:413-421 pubmed publisher
    We investigate a ternary system that consists of itraconazole (ITZ) and two polymers: povidone K12 and Carbopol 907...
  41. Ogasawara M, Matsumoto Y, Hayashi T, Ohno K, Yamada H, Kawakita T, et al. KRT12 mutations and in vivo confocal microscopy in two Japanese families with Meesmann corneal dystrophy. Am J Ophthalmol. 2014;157:93-102.e1 pubmed publisher
    ..The in vivo confocal microscopy examinations revealed previously unreported depth-dependent ultrastructural changes in the living cornea of Meesmann corneal dystrophy patients. ..
  42. Akkermans S, Noriega Fernández E, Logist F, Van Impe J. Introducing a novel interaction model structure for the combined effect of temperature and pH on the microbial growth rate. Int J Food Microbiol. 2017;240:85-96 pubmed publisher
    ..As a case study, the effect of temperature and pH on the growth rate of Escherichia coli K12 is modeled, based on a set of computer controlled bioreactor experiments performed under static environmental ..
  43. Scheithauer S, Hafner H, Seef R, Seef S, Hilgers R, Lemmen S. Disinfection of gloves: feasible, but pay attention to the disinfectant/glove combination. J Hosp Infect. 2016;94:268-272 pubmed publisher
    ..coli K12 NCTC 10538, (2) recovery rates after contamination, (3) reduction efficacy, (4) fingertip immersion culture, and (5)..
  44. Botto S, Totonchy J, Gustin J, Moses A. Kaposi Sarcoma Herpesvirus Induces HO-1 during De Novo Infection of Endothelial Cells via Viral miRNA-Dependent and -Independent Mechanisms. MBio. 2015;6:e00668 pubmed publisher
    ..A viral microRNA miR-K12-11 deletion mutant of KSHV was found to be defective for induction of HO-1 during latency...
  45. Sun F, Ding J, Lü X, Gao R, Lu X, Shi E, et al. Mass spectral characterization of tabun-labeled lysine biomarkers in albumin. J Chromatogr B Analyt Technol Biomed Life Sci. 2017;1057:54-61 pubmed publisher
    ..types of albumin were treated with overdose of tabun in vitro and 17 tabun-labeled lysine residues were found: K4, K12, K224, K377, and K524 in bovine albumin, K186, K188, K212, K329, K414, and K525 in leporine albumin, and K79, K186, ..
  46. Abdel Mohsen A, Jancar J, Abdel Rahman R, Vojtek L, Hyrsl P, Duskova M, et al. A novel in situ silver/hyaluronan bio-nanocomposite fabrics for wound and chronic ulcer dressing: In vitro and in vivo evaluations. Int J Pharm. 2017;520:241-253 pubmed publisher
    ..The antibacterial performance of medical HA/Ag-NPs fabrics was evaluated against gram negative bacteria E. coli K12, exhibiting significant bactericidal activity...
  47. Maphis N, Jiang S, Binder J, Wright C, Gopalan B, Lamb B, et al. Whole Genome Expression Analysis in a Mouse Model of Tauopathy Identifies MECP2 as a Possible Regulator of Tau Pathology. Front Mol Neurosci. 2017;10:69 pubmed publisher
    ..Together, these results suggest that MECP2 is a potential novel regulator of tau pathology relevant to AD and tauopathies. ..
  48. den Breems N, Eftimie R. The re-polarisation of M2 and M1 macrophages and its role on cancer outcomes. J Theor Biol. 2016;390:23-39 pubmed publisher
    ..Moreover, we show that (i) the ratio k of the transition rates k12 (for the re-polarisation M1→M2) and k21 (for the re-polarisation M2→M1) is important in reducing ..
  49. Steiner B, Eberly A, Hurst M, Zhang E, Green H, Behr S, et al. Evidence of Cross-Regulation in Two Closely Related Pyruvate-Sensing Systems in Uropathogenic Escherichia coli. J Membr Biol. 2018;251:65-74 pubmed publisher
    ..The YpdA/YpdB and BtsS/BtsR TCSs have been previously reported to interact in K12 E. coli, where they alter the expression of putative transporter genes yhjX and yjiY, respectively...
  50. Gao W, Ma H, Zheng D, Dong Z, Wu L, Bi L. An effective combination of electrodeposition and layer-by-layer assembly to construct composite films with luminescence switching behavior. Dalton Trans. 2015;44:14763-70 pubmed publisher
    ..Then, the multilayer films containing different layers of tungstophosphate K12.5Na1...
  51. Tiwari A, Loughner C, Swamynathan S, Swamynathan S. KLF4 Plays an Essential Role in Corneal Epithelial Homeostasis by Promoting Epithelial Cell Fate and Suppressing Epithelial-Mesenchymal Transition. Invest Ophthalmol Vis Sci. 2017;58:2785-2795 pubmed publisher
    ..Collectively, these results reveal that KLF4 plays an essential role in CE homeostasis by promoting epithelial cell fate and suppressing EMT. ..
  52. Pohl S, Madzgalla M, Manz W, Bart H. Biofouling on polymeric heat exchanger surfaces with E. coli and native biofilms. Biofouling. 2015;31:699-707 pubmed publisher
    ..polyether ether ketone) in comparison to stainless steel (SS) was studied for the model bacterium Escherichia coli K12 DSM 498 and native biofilms originating from Rhine water...
  53. Jain V, Plaisance Bonstaff K, Sangani R, Lanier C, Dolce A, Hu J, et al. A Toolbox for Herpesvirus miRNA Research: Construction of a Complete Set of KSHV miRNA Deletion Mutants. Viruses. 2016;8: pubmed publisher
    ..lines based on iSLK cells have been created for wildtype KSHV, for 12 individual miRNA knock-out mutants (ΔmiR-K12-1 through -12), and for mutants deleted for 10 of 12 (ΔmiR-cluster) or all 12 miRNAs (ΔmiR-all)...
  54. Yan Z, Yin M, Xu D, Zhu Y, Li X. Structural insights into the secretin translocation channel in the type II secretion system. Nat Struct Mol Biol. 2017;24:177-183 pubmed publisher
    ..Here we report cryo-EM structures of two GspD channels (∼1 MDa), from Escherichia coli K12 and Vibrio cholerae, at ∼3 Å resolution...
  55. Lin L, Fu C, Huang W. Improving the activity of the endoglucanase, Cel8M from Escherichia coli by error-prone PCR. Enzyme Microb Technol. 2016;86:52-8 pubmed publisher
    ..coli K12. Through screening of approximately 10,000Cel8M variants, two variants, Cel8ME15 and Cel8ME18, respectively showing ..
  56. Shinohara N, Oshima Y, Kobayashi T, Imatanaka N, Nakai M, Ichinose T, et al. Dose-dependent clearance kinetics of intratracheally administered titanium dioxide nanoparticles in rat lung. Toxicology. 2014;325:1-11 pubmed publisher
    ..5-6.0 mg/kg (0.014-0.022/day). The translocation rate constants from compartment 1 to 2, k12, were estimated to be 0.015 and 0.018/day for the 0.375 and 0.75 mg/kg doses, and 0.0025-0.0092/day for doses of 1...
  57. Lui G, Roser D, Corkish R, Ashbolt N, Stuetz R. Point-of-use water disinfection using ultraviolet and visible light-emitting diodes. Sci Total Environ. 2016;553:626-635 pubmed publisher
    ..or semi-commercial LED arrays (270-740 nm) were compared for their ability to inactivate Escherichia coli K12 ATCC W3110 and Enterococcus faecalis ATCC 19433 over 6h...
  58. Okeigwe I, Wang C, Politch J, Heffner L, Kuohung W. Physician-scientists in obstetrics and gynecology: predictors of success in obtaining independent research funding. Am J Obstet Gynecol. 2017;217:84.e1-84.e8 pubmed publisher
    ..The purpose of this study is to describe the cohort of obstetrics and gynecology physician-scientists who were K08, K12, and K23 recipients from 1988 through 2015 and to identify predictors of success in obtaining independent federal ..
  59. Varik V, Oliveira S, Hauryliuk V, Tenson T. HPLC-based quantification of bacterial housekeeping nucleotides and alarmone messengers ppGpp and pppGpp. Sci Rep. 2017;7:11022 pubmed publisher
    ..We applied our method to quantify nucleotide pools in Escherichia coli BW25113 K12-strain both throughout the growth curve and during acute stringent response induced by mupirocin...
  60. Zhang W, Radadia A. Toward a Boron-Doped Ultrananocrystalline Diamond Electrode-Based Dielectrophoretic Preconcentrator. Anal Chem. 2016;88:2605-13 pubmed publisher
    ..coli O157:H7 colony-forming units (cfu)/mL, or ∼500 E. coli O157:H7 and ∼500 E. coli K12 cfu/mL...
  61. Scotti R, Nicolini L, Gabbianelli R. sodC genes expression in Escherichia coli O157:H7 strains. Ann Ist Super Sanita. 2016;52:309-12 pubmed publisher
    ..coli K12 strain. The expression of the chromosomal gene was always higher than that of the prophagic copies...
  62. Zhang Z, Kukita C, Humayun M, Saier M. Environment-directed activation of the Escherichia coliflhDC operon by transposons. Microbiology. 2017;163:554-569 pubmed publisher
    The flagellar system in Escherichia coli K12 is expressed under the control of the flhDC-encoded master regulator FlhDC...
  63. Fang S, Wang L, Fang Q, Chen C, Zhao X, Qian C, et al. Characterization and functional study of a Cecropin-like peptide from the Chinese oak silkworm, Antheraea pernyi. Arch Insect Biochem Physiol. 2017;94: pubmed publisher
    ..The antibacterial activity of M-ApCec is more potent than pro-ApCec against E. coli K12 or B. subtilus in both minimum inhibitory concentration and inhibition zone assays...
  64. Akkermans S, Logist F, Van Impe J. An interaction model for the combined effect of temperature, pH and water activity on the growth rate of E. coli K12. Food Res Int. 2018;106:1123-1131 pubmed publisher
    ..coli K12 based on a dataset of 68 bioreactor experiments...
  65. Latta L, Viestenz A, Stachon T, Colanesi S, Szentmary N, Seitz B, et al. Human aniridia limbal epithelial cells lack expression of keratins K3 and K12. Exp Eye Res. 2018;167:100-109 pubmed publisher
    ..factor, PAX6, potential stem cell markers, ΔNp63α and ABCG2, and corneal differentiation markers, keratin 12 (K12) and K3. Conjunctival differentiation markers, keratin 13 (K13) and K19 were also investigated...
  66. Allen E, Atkinson S, Liao H, Moore J, Pedrioli D, Smith F, et al. Allele-specific siRNA silencing for the common keratin 12 founder mutation in Meesmann epithelial corneal dystrophy. Invest Ophthalmol Vis Sci. 2013;54:494-502 pubmed publisher
    ..siRNAs against the K12 Arg135Thr mutation were evaluated using a dual luciferase reporter gene assay and the most potent and specific ..
  67. Manrique Y, Gibis M, Schmidt H, Weiss J. Influence of application sequence and timing of eugenol and lauric arginate (LAE) on survival of spoilage organisms. Food Microbiol. 2017;64:210-218 pubmed publisher
    ..and lauric arginate (LAE) was investigated against Staphylococcus carnosus, Listeria innocua, Escherichia coli K12, and Pseudomonas fluorescens...
  68. Cannon C, Trembley J, Kren B, Unger G, O Sullivan M, Cornax I, et al. Evaluation of protein kinase CK2 as a therapeutic target for squamous cell carcinoma of cats. Am J Vet Res. 2017;78:946-953 pubmed publisher
    ..clinically normal cats, biopsy specimens of oral SCC from cats, and feline SCC (SCCF1) and mammary gland carcinoma (K12) cell lines. PROCEDURES Immunohistochemical labeling for CK2? was performed on biopsy specimens...
  69. Satoh A, Mitogawa K, Saito N, Suzuki M, Suzuki K, Ochi H, et al. Reactivation of larval keratin gene (krt62.L) in blastema epithelium during Xenopus froglet limb regeneration. Dev Biol. 2017;432:265-272 pubmed publisher
    ..L-positive froglet blastema epithelium is able to support the limb development process. These findings imply that the developmental process is locally reactivated in an postmetamorphic body during limb regeneration. ..
  70. Zhou Y, Zhou J, Ji Y, Li L, Tan Y, Tian G, et al. Bioluminescent tracing of a Yersinia pestis pCD1+-mutant and Yersinia pseudotuberculosis in subcutaneously infected mice. Microbes Infect. 2018;20:166-175 pubmed publisher
    ..pseudotuberculosis or Escherichia coli K12 to construct stable bioluminescent strains for investigation of their dissemination in mice by bioluminescence ..
  71. Bury D, Belov V, Qi Y, Hayen H, Volmer D, Bruning T, et al. Determination of urinary metabolites of the emerging UV filter Octocrylene by online-SPE-LC-MS/MS. Anal Chem. 2017;: pubmed publisher
    ..Urine samples are incubated with ?-glucuronidase (E. coli K12) and then analyzed by liquid chromatography-electrospray ionization-triple quadrupole-tandem mass spectrometry with ..
  72. Loukanov A, Mladenova P, Toshev S, Udono H, Nakabayashi S. Visualization of the native shape of bodipy-labeled DNA in Escherichia coli by correlative microscopy. Microsc Res Tech. 2017;: pubmed publisher
    ..coli mutant K12 strain, which cannot synthesize thymine...
  73. Duplay P, Szmelcman S, Bedouelle H, Hofnung M. Silent and functional changes in the periplasmic maltose-binding protein of Escherichia coli K12. I. Transport of maltose. J Mol Biol. 1987;194:663-73 pubmed
    ..A region near the C-terminal end is important for maltose binding. Two regions of the mature protein (residues 18 to 42 and 280 to 296) are required for export to, or solubility in, the periplasm...
  74. Gilson E, Nikaido H, Hofnung M. Sequence of the malK gene in E.coli K12. Nucleic Acids Res. 1982;10:7449-58 pubmed
    We present the sequence of gene malK which encodes a component of the system for maltose transport in E.coli K12. We also determined the position of deletion (S50) which fuses malK to the following gene lamB; the malK-lamB protein hybrid ..
  75. Du X, Chen P, Sun D. Mutation analysis of TGFBI and KRT12 in a case of concomitant keratoconus and granular corneal dystrophy. Graefes Arch Clin Exp Ophthalmol. 2017;255:1779-1786 pubmed publisher
    ..KC can co-exist with GCD. The missense mutation (c.370G > A) in the TGFBI gene and insert mutation (c.1456-1457ins GAT) in the KRT12 gene were identified in a 23-year-old male patient with concurrent KC and GCD. ..
  76. Rieger K, Porter M, Schiffman J. Polyelectrolyte-Functionalized Nanofiber Mats Control the Collection and Inactivation of Escherichia coli. Materials (Basel). 2016;9: pubmed publisher
    ..In this study, the collection and inactivation of Escherichia coli K12 was examined using cellulose nanofiber mats that were surface-functionalized using three polyelectrolytes: poly (..
  77. Evseeva N, Tkachenko O, Burygin G, Matora L, Lobachev Y, Shchyogolev S. Effect of bacterial lipopolysaccharides on morphogenetic activity in wheat somatic calluses. World J Microbiol Biotechnol. 2017;34:3 pubmed publisher
    ..associative bacterium Azospirillum brasilense Sp245 and from the enteric bacterium Escherichia coli K12 on the morphogenic potential of in vitro-growing somatic calluses of soft spring wheat (Triticum aestivum L. cv...
  78. Nishida K, Adachi W, Shimizu Matsumoto A, Kinoshita S, Mizuno K, Matsubara K, et al. A gene expression profile of human corneal epithelium and the isolation of human keratin 12 cDNA. Invest Ophthalmol Vis Sci. 1996;37:1800-9 pubmed
    ..GS8025), isolated from a separately made cDNA library, contained open reading frame highly homologous to mouse keratin 12, which is known to be cornea specific...
  79. Biswas P, Bandyopadhyaya R. Water disinfection using silver nanoparticle impregnated activated carbon: Escherichia coli cell-killing in batch and continuous packed column operation over a long duration. Water Res. 2016;100:105-115 pubmed publisher
    ..e. batch-mode) and flow-column (i. e. continuous-mode) operations, using E. coli K12 (MTCC 1302) as a model organism...
  80. Pathak M, Olstad O, Drolsum L, Moe M, Smorodinova N, Kalasova S, et al. The effect of culture medium and carrier on explant culture of human limbal epithelium: A comparison of ultrastructure, keratin profile and gene expression. Exp Eye Res. 2016;153:122-132 pubmed publisher
    ..light microscopy (LM), transmission electron microscopy (TEM), immunohistochemistry (IHC) for keratins K3, K7, K8, K12, K13, K14, K15 and K19, as well as microarray and qRT-PCR analysis...
  81. Monteagudo J, Duran A, Martín I, Acevedo A. A novel combined solar pasteurizer/TiO2 continuous-flow reactor for decontamination and disinfection of drinking water. Chemosphere. 2017;168:1447-1456 pubmed publisher
    ..by studying the effect of heat treatment on the death kinetic parameters (D and z values) of Escherichia coli K12 (CECT 4624). 99.1% bacteria photo-inactivation was reached in the TiO2-CPC system (0...
  82. Dassa E, Hofnung M. Sequence of gene malG in E. coli K12: homologies between integral membrane components from binding protein-dependent transport systems. EMBO J. 1985;4:2287-93 pubmed
    The MalG protein is needed for the transport of maltose in Escherichia coli K12. We present the sequence of gene malG. The deduced amino acid sequence corresponds to a protein of 296 amino acid residues (mol. wt. = 32 188 daltons)...
  83. Courtney D, Atkinson S, Allen E, Moore J, Walsh C, Pedrioli D, et al. siRNA silencing of the mutant keratin 12 allele in corneal limbal epithelial cells grown from patients with Meesmann's epithelial corneal dystrophy. Invest Ophthalmol Vis Sci. 2014;55:3352-60 pubmed publisher
    The aim of this study is to further assess our previously reported keratin 12 (K12)-Leu132Pro specific siRNA in silencing the mutant allele in Meesmann's Epithelial Corneal Dystrophy (MECD) in experimental systems more akin to the in vivo ..
  84. Sasamoto Y, Hayashi R, Park S, Saito Adachi M, Suzuki Y, Kawasaki S, et al. PAX6 Isoforms, along with Reprogramming Factors, Differentially Regulate the Induction of Cornea-specific Genes. Sci Rep. 2016;6:20807 pubmed publisher
    ..of genes specific to the structure and functions of the corneal epithelium, particularly keratin 3 (KRT3) and keratin 12 (KRT12). PAX6 isoform-a induced KRT3 expression by targeting its upstream region. KLF4 enhanced this induction...
  85. Li X, Qi C, Wei P, Huang L, Cai J, Xu Z. Efficient chemoenzymatic synthesis of uridine 5'-diphosphate N-acetylglucosamine and uridine 5'-diphosphate N-trifluoacetyl glucosamine with three recombinant enzymes. Prep Biochem Biotechnol. 2017;47:852-859 pubmed publisher
    ..N-acetylglucosamine-1-phosphate uridyltransferase from Escherichia coli K12 MG1655 was used to produce UDP-GlcNAc/GlcNTFA from GlcNAc/GlcNTFA-1-phosphate. Inorganic pyrophosphatase from E...
  86. Zhao X, Guo L, Lu X, Lu H, Wang F, Zhong M, et al. Evidences of abundant hemocyanin variants in shrimp Litopenaeus vannamei. Mol Immunol. 2016;77:103-12 pubmed publisher
    ..distribution, which were affected by stimulation with six pathogenic bacteria, including Escherichia coli K12, Vibrio parahaemolyticus, Vibrio alginolyticus, Vibrio fluvialis, Streptococcus pyogenes and Staphylococcus aureus, ..
  87. Trivillin V, Pozzi E, Colombo L, Thorp S, Garabalino M, Monti Hughes A, et al. Abscopal effect of boron neutron capture therapy (BNCT): proof of principle in an experimental model of colon cancer. Radiat Environ Biophys. 2017;56:365-375 pubmed publisher
    ..Twenty-six BDIX rats were inoculated subcutaneously with 1 × 106 DHD/K12/TRb syngeneic colon cancer cells in the right hind flank...
  88. Jiang T, Zhang C, He Q, Zheng Z, Ouyang J. Metabolic Engineering of Escherichia coli K12 for Homofermentative Production of L-Lactate from Xylose. Appl Biochem Biotechnol. 2018;184:703-715 pubmed publisher
    ..90, 20.13, and 233.88% relative to the control, respectively. This was the first report that adding formate not only could increase the xylose utilization but also led to the fewer by-product levels. ..
  89. Yuan P, D Lima N, Slavoff S. Comparative Membrane Proteomics Reveals a Nonannotated E. coli Heat Shock Protein. Biochemistry. 2018;57:56-60 pubmed publisher
    ..quantitative proteomics protocol, revealing a never-before-identified heat shock protein in Escherichia coli K12. This putative smORF-encoded heat shock protein, GndA, is likely to be ∼36-55 amino acids in length and contains ..
  90. Shuman H. Active transport of maltose in Escherichia coli K12. Role of the periplasmic maltose-binding protein and evidence for a substrate recognition site in the cytoplasmic membrane. J Biol Chem. 1982;257:5455-61 pubmed
    ..A model for the operation of the transport system is presented. In this model, the substrate recognition site in the cytoplasmic membrane is exposed to alternate sides of the membrane...
  91. Li D, Chen Z, Song X, de Paiva C, Kim H, Pflugfelder S. Partial enrichment of a population of human limbal epithelial cells with putative stem cell properties based on collagen type IV adhesiveness. Exp Eye Res. 2005;80:581-90 pubmed
    ..BrdU-label retention, (b) expression of basal cell (integrin beta1, p63, ABCG2) and differentiation (involucrin, keratin 12) markers, and (c) colony forming efficiency (CFE) and growth capacity on a 3T3 fibroblast feeder layer...
  92. Yang Y, Ballent W, Mayer B. High-affinity phosphate-binding protein (PBP) for phosphorous recovery: proof of concept using recombinant Escherichia coli. FEMS Microbiol Lett. 2016;363: pubmed
    ..coli and E. coli K12). Lower pH (3...