integrin linked kinase

Summary

Gene Symbol: integrin linked kinase
Description: integrin linked kinase
Alias: HEL-S-28, ILK-1, ILK-2, P59, p59ILK, integrin-linked protein kinase, 59 kDa serine/threonine-protein kinase, epididymis secretory protein Li 28, integrin-linked kinase-2
Species: human
Products:     integrin linked kinase

Top Publications

  1. Nikolopoulos S, Turner C. Molecular dissection of actopaxin-integrin-linked kinase-Paxillin interactions and their role in subcellular localization. J Biol Chem. 2002;277:1568-75 pubmed
    ..This mutant also exhibited in vitro kinase activity comparable with wild-type ILK. Taken together, these data suggest that normal ILK signaling is dependent on efficient localization involving multiple protein interactions. ..
  2. Persad S, Dedhar S. The role of integrin-linked kinase (ILK) in cancer progression. Cancer Metastasis Rev. 2003;22:375-84 pubmed
    ..In this review, we summarize the adaptor and signaling properties ofILK, and also progress in the identification of therapeutic strategies for inhibition of ILK activity. ..
  3. Kim M, Ogawa M, Fujita Y, Yoshikawa Y, Nagai T, Koyama T, et al. Bacteria hijack integrin-linked kinase to stabilize focal adhesions and block cell detachment. Nature. 2009;459:578-82 pubmed publisher
    ..These results indicate that Shigella sustain their infectious foothold by using special tactics to prevent detachment of infected cells. ..
  4. Durbin A, Pasic I, Wong D, Hannigan G, Malkin D. The oncogenic and growth-suppressive functions of the integrin-linked kinase are distinguished by JNK1 expression in human cancer cells. Cell Cycle. 2010;9:1951-9 pubmed
    ..These results provide a rationale for stratifying patients to receive ILK kinase inhibitors based on individualized tumor-specific ILK function. ..
  5. Tu Y, Li F, Goicoechea S, Wu C. The LIM-only protein PINCH directly interacts with integrin-linked kinase and is recruited to integrin-rich sites in spreading cells. Mol Cell Biol. 1999;19:2425-34 pubmed
  6. Sakai T, Li S, Docheva D, Grashoff C, Sakai K, Kostka G, et al. Integrin-linked kinase (ILK) is required for polarizing the epiblast, cell adhesion, and controlling actin accumulation. Genes Dev. 2003;17:926-40 pubmed
    ..Altogether these data show that mammalian ILK modulates actin rearrangements at integrin-adhesion sites. ..
  7. Ahmed N, Oliva K, Rice G, Quinn M. Cell-free 59 kDa immunoreactive integrin-linked kinase: a novel marker for ovarian carcinoma. Clin Cancer Res. 2004;10:2415-20 pubmed
    ..These data suggest that irILK is an ovarian tumor-associated antigen and implicates its potential not only as a biomarker for early-stage screening but also as a marker for monitoring the clinical condition of patients after treatment. ..
  8. Li Y, Dai C, Wu C, Liu Y. PINCH-1 promotes tubular epithelial-to-mesenchymal transition by interacting with integrin-linked kinase. J Am Soc Nephrol. 2007;18:2534-43 pubmed
    ..We conclude that PINCH-1, through its interaction with ILK, plays an important role in regulating TGF-beta1-mediated EMT and could be a potential future therapeutic target to prevent progression of renal disease. ..
  9. Li Y, Yang J, Dai C, Wu C, Liu Y. Role for integrin-linked kinase in mediating tubular epithelial to mesenchymal transition and renal interstitial fibrogenesis. J Clin Invest. 2003;112:503-16 pubmed
    ..These findings suggest that ILK is a critical mediator for tubular EMT and likely plays a crucial role in the pathogenesis of chronic renal fibrosis. ..

More Information

Publications78

  1. Dai D, Makretsov N, Campos E, Huang C, Zhou Y, Huntsman D, et al. Increased expression of integrin-linked kinase is correlated with melanoma progression and poor patient survival. Clin Cancer Res. 2003;9:4409-14 pubmed
    ..05). ILK expression increases dramatically with melanoma invasion and progression and is inversely correlated with patient survival. ..
  2. McDonald P, Oloumi A, Mills J, Dobreva I, Maidan M, Gray V, et al. Rictor and integrin-linked kinase interact and regulate Akt phosphorylation and cancer cell survival. Cancer Res. 2008;68:1618-24 pubmed publisher
    ..These data show that rictor regulates the ability of ILK to promote Akt phosphorylation and cancer cell survival. ..
  3. Matsui Y, Assi K, Ogawa O, Raven P, Dedhar S, Gleave M, et al. The importance of integrin-linked kinase in the regulation of bladder cancer invasion. Int J Cancer. 2012;130:521-31 pubmed publisher
    ..ILK may be a new molecular target to suppress tumor progression in advanced and high-risk bladder cancer patients. ..
  4. Abboud E, Coffelt S, Figueroa Y, Zwezdaryk K, Nelson A, Sullivan D, et al. Integrin-linked kinase: a hypoxia-induced anti-apoptotic factor exploited by cancer cells. Int J Oncol. 2007;30:113-22 pubmed
    ..The identification of ILK as a player in hypoxia survival signaling employed by cancer cells further validates ILK as a unique target for cancer therapy. ..
  5. Delcommenne M, Tan C, Gray V, Rue L, Woodgett J, Dedhar S. Phosphoinositide-3-OH kinase-dependent regulation of glycogen synthase kinase 3 and protein kinase B/AKT by the integrin-linked kinase. Proc Natl Acad Sci U S A. 1998;95:11211-6 pubmed
    ..ILK is thus a receptor-proximal effector for the Pi(3)K-dependent, extracellular matrix and growth factor mediated, activation of PKB/AKT, and inhibition of GSK-3. ..
  6. Attwell S, Mills J, Troussard A, Wu C, Dedhar S. Integration of cell attachment, cytoskeletal localization, and signaling by integrin-linked kinase (ILK), CH-ILKBP, and the tumor suppressor PTEN. Mol Biol Cell. 2003;14:4813-25 pubmed
    ..These data demonstrate that the kinase and adaptor properties of ILK function together, in a Pi3 kinase-dependent manner, to regulate integrin-mediated cell attachment and signal transduction. ..
  7. Lu H, Fedak P, Dai X, Du C, Zhou Y, Henkelman M, et al. Integrin-linked kinase expression is elevated in human cardiac hypertrophy and induces hypertrophy in transgenic mice. Circulation. 2006;114:2271-9 pubmed
    ..Taken together, these results identify ILK-regulated signaling as a broadly adaptive hypertrophic response mechanism relevant to a wide range of clinical heart disease. ..
  8. McPhee T, McDonald P, Oloumi A, Dedhar S. Integrin-linked kinase regulates E-cadherin expression through PARP-1. Dev Dyn. 2008;237:2737-47 pubmed publisher
    ..We suggest a model in which ILK represses E-cadherin expression by regulating PARP-1, leading to the binding of PARP-1 to SIRE and modulation of Snail expression. ..
  9. Lynch D, Ellis C, Edwards P, Hiles I. Integrin-linked kinase regulates phosphorylation of serine 473 of protein kinase B by an indirect mechanism. Oncogene. 1999;18:8024-32 pubmed
    ..These data strongly suggest that integrin-linked kinase does not possess serine-473 kinase activity but functions as an adaptor to recruit a serine-473 kinase or phosphatase. ..
  10. Maydan M, McDonald P, Sanghera J, Yan J, Rallis C, Pinchin S, et al. Integrin-linked kinase is a functional Mn2+-dependent protein kinase that regulates glycogen synthase kinase-3? (GSK-3beta) phosphorylation. PLoS ONE. 2010;5:e12356 pubmed publisher
    ..Taken together, our data demonstrate that ILK is a bona fide protein kinase with enzyme kinetic properties similar to other active protein kinases. ..
  11. Tan C, Mui A, Dedhar S. Integrin-linked kinase regulates inducible nitric oxide synthase and cyclooxygenase-2 expression in an NF-kappa B-dependent manner. J Biol Chem. 2002;277:3109-16 pubmed
    ..Furthermore, we also demonstrate that ILK activity is required for LPS stimulated cyclooxygenase-2 expression in murine and human macrophages. These findings implicate ILK as a potential target for anti-inflammatory applications. ..
  12. Mills J, Niewmierzycka A, Oloumi A, Rico B, St Arnaud R, Mackenzie I, et al. Critical role of integrin-linked kinase in granule cell precursor proliferation and cerebellar development. J Neurosci. 2006;26:830-40 pubmed
    ..Together, these results implicate ILK as a critical effector in a signaling pathway necessary for granule cell proliferation and cerebellar development. ..
  13. Zhang Y, Chen K, Tu Y, Velyvis A, Yang Y, Qin J, et al. Assembly of the PINCH-ILK-CH-ILKBP complex precedes and is essential for localization of each component to cell-matrix adhesion sites. J Cell Sci. 2002;115:4777-86 pubmed
    ..These results provide new insights into the molecular mechanism underlying the assembly and regulation of cell-matrix adhesion structures. ..
  14. Herranz B, Marquez S, Guijarro B, Aracil E, Aicart Ramos C, Rodríguez Crespo I, et al. Integrin-linked kinase regulates vasomotor function by preventing endothelial nitric oxide synthase uncoupling: role in atherosclerosis. Circ Res. 2012;110:439-49 pubmed publisher
    ..Our results identify ILK as a regulatory partner of eNOS in vivo that prevents eNOS uncoupling, and suggest ILK as a therapeutic target for prevention of endothelial dysfunction related to shear stress-induced vascular diseases. ..
  15. Tan C, Cruet Hennequart S, Troussard A, Fazli L, Costello P, Sutton K, et al. Regulation of tumor angiogenesis by integrin-linked kinase (ILK). Cancer Cell. 2004;5:79-90 pubmed
    ..These data demonstrate an important and essential role of ILK in two key aspects of tumor angiogenesis: VEGF expression by tumor cells and VEGF-stimulated blood vessel formation. ..
  16. Knoll R, Postel R, Wang J, Krätzner R, Hennecke G, Vacaru A, et al. Laminin-alpha4 and integrin-linked kinase mutations cause human cardiomyopathy via simultaneous defects in cardiomyocytes and endothelial cells. Circulation. 2007;116:515-25 pubmed
  17. Fukuda T, Guo L, Shi X, Wu C. CH-ILKBP regulates cell survival by facilitating the membrane translocation of protein kinase B/Akt. J Cell Biol. 2003;160:1001-8 pubmed
  18. Chen Z, Yang A, Xu C, Xing Y, Gong W, Li J. c-Jun N-terminal kinase is involved in the regulation of proliferation and apoptosis by integrin-linked kinase in human retinoblastoma cells. Graefes Arch Clin Exp Ophthalmol. 2011;249:1399-407 pubmed publisher
    ..This might provide a potential therapeutic target in the treatment of this deadly disease. ..
  19. Wani A, Jafarnejad S, Zhou J, Li G. Integrin-linked kinase regulates melanoma angiogenesis by activating NF-?B/interleukin-6 signaling pathway. Oncogene. 2011;30:2778-88 pubmed publisher
  20. Gagné D, Groulx J, Benoit Y, Basora N, Herring E, Vachon P, et al. Integrin-linked kinase regulates migration and proliferation of human intestinal cells under a fibronectin-dependent mechanism. J Cell Physiol. 2010;222:387-400 pubmed publisher
  21. Troussard A, Mawji N, Ong C, Mui A, St Arnaud R, Dedhar S. Conditional knock-out of integrin-linked kinase demonstrates an essential role in protein kinase B/Akt activation. J Biol Chem. 2003;278:22374-8 pubmed
    ..ILK knock-out also resulted in the suppression of phosphorylation of GSK-3beta on Ser-9 and cyclin D1 expression. These data establish ILK as an essential upstream regulator of PKB/Akt activation. ..
  22. Fukuda K, Gupta S, Chen K, Wu C, Qin J. The pseudoactive site of ILK is essential for its binding to alpha-Parvin and localization to focal adhesions. Mol Cell. 2009;36:819-30 pubmed publisher
    ..They also highlight functional diversity of the kinase fold and its "active" site in mediating many biological processes. ..
  23. Hill M, Feng J, Hemmings B. Identification of a plasma membrane Raft-associated PKB Ser473 kinase activity that is distinct from ILK and PDK1. Curr Biol. 2002;12:1251-5 pubmed
    ..In contrast to previous observations, however, ILK immunoprecipitates did not retain Ser473 kinase activity. Thus, we have identified a novel raft-associated PKB Ser473 kinase, implicating a role for lipid rafts in PKB signaling. ..
  24. Durbin A, Somers G, Forrester M, Pienkowska M, Hannigan G, Malkin D. JNK1 determines the oncogenic or tumor-suppressive activity of the integrin-linked kinase in human rhabdomyosarcoma. J Clin Invest. 2009;119:1558-70 pubmed publisher
  25. Fielding A, Lim S, Montgomery K, Dobreva I, Dedhar S. A critical role of integrin-linked kinase, ch-TOG and TACC3 in centrosome clustering in cancer cells. Oncogene. 2011;30:521-34 pubmed publisher
    ..In addition, we identify a specific TACC3 phosphorylation site that is required for centrosome clustering and demonstrate that ILK regulates this phosphorylation in an Aurora-A-dependent manner...
  26. Guo L, Sanders P, Woods A, Wu C. The distribution and regulation of integrin-linked kinase in normal and diabetic kidneys. Am J Pathol. 2001;159:1735-42 pubmed
    ..These results suggest that ILK is likely involved in mesangial matrix expansion in response to hyperglycemia in the pathogenesis of diabetic glomerulosclerosis. ..
  27. Fielding A, Dobreva I, McDonald P, Foster L, Dedhar S. Integrin-linked kinase localizes to the centrosome and regulates mitotic spindle organization. J Cell Biol. 2008;180:681-9 pubmed publisher
    ..These data demonstrate a critical and unexpected function for ILK in the organization of centrosomal protein complexes during mitotic spindle assembly and DNA segregation. ..
  28. Tu Y, Huang Y, Zhang Y, Hua Y, Wu C. A new focal adhesion protein that interacts with integrin-linked kinase and regulates cell adhesion and spreading. J Cell Biol. 2001;153:585-98 pubmed
    ..These findings reveal a novel CH-ILKBP-ILK-PINCH complex and provide important evidence for a crucial role of this complex in the regulation of cell adhesion and cytoskeleton organization. ..
  29. Lange A, Wickström S, Jakobson M, Zent R, Sainio K, Fassler R. Integrin-linked kinase is an adaptor with essential functions during mouse development. Nature. 2009;461:1002-6 pubmed publisher
    ..Thus, we provide genetic evidence that the kinase activity of Ilk is dispensable for mammalian development; however, an interaction between Ilk and alpha-parvin is critical for kidney development. ..
  30. Hannigan G, Bayani J, Weksberg R, Beatty B, Pandita A, Dedhar S, et al. Mapping of the gene encoding the integrin-linked kinase, ILK, to human chromosome 11p15.5-p15.4. Genomics. 1997;42:177-9 pubmed
    We have recently reported the identification and cloning of the gene encoding p59ILK, a novel protein ser/thr kinase that is found in physiologic complexes with beta integrin subunits...
  31. Medici D, Nawshad A. Type I collagen promotes epithelial-mesenchymal transition through ILK-dependent activation of NF-kappaB and LEF-1. Matrix Biol. 2010;29:161-5 pubmed publisher
    ..These data provide a foundation for understanding the mechanisms by which collagen I stimulates EMT and identify potential therapeutic targets for suppressing this transition in pathological conditions. ..
  32. Serrano I, McDonald P, Lock F, Dedhar S. Role of the integrin-linked kinase (ILK)/Rictor complex in TGF?-1-induced epithelial-mesenchymal transition (EMT). Oncogene. 2013;32:50-60 pubmed publisher
    ..These data demonstrate a requirement for ILK function in TGF?-1-induced EMT in mammary epithelial cells and identify the ILK/Rictor complex as a potential molecular target for preventing/reversing EMT. ..
  33. Lee S, Youn S, Cho H, Li L, Kim T, Yook H, et al. Integrin-linked kinase, a hypoxia-responsive molecule, controls postnatal vasculogenesis by recruitment of endothelial progenitor cells to ischemic tissue. Circulation. 2006;114:150-9 pubmed
    ..Endogenous ILK is a novel and physiological upstream responder of numerous intracellular molecules involved in hypoxic stress in ECs and may control the recruitment of EPCs to ischemic tissue. ..
  34. Oinuma I, Katoh H, Negishi M. R-Ras controls axon specification upstream of glycogen synthase kinase-3beta through integrin-linked kinase. J Biol Chem. 2007;282:303-18 pubmed
    ..In addition, membrane targeting of ILK was sufficient to inactivate GSK-3beta and to form multiple axons. Our study demonstrates a novel role of R-Ras and ILK upstream of GSK-3beta in the regulation of neuronal polarity. ..
  35. Duxbury M, Ito H, Benoit E, Waseem T, Ashley S, Whang E. RNA interference demonstrates a novel role for integrin-linked kinase as a determinant of pancreatic adenocarcinoma cell gemcitabine chemoresistance. Clin Cancer Res. 2005;11:3433-8 pubmed
    ..This novel finding suggests that therapies directed against ILK and its downstream signaling targets may have the potential to enhance the efficacy of gemcitabine-based chemotherapy. ..
  36. Persad S, Attwell S, Gray V, Mawji N, Deng J, Leung D, et al. Regulation of protein kinase B/Akt-serine 473 phosphorylation by integrin-linked kinase: critical roles for kinase activity and amino acids arginine 211 and serine 343. J Biol Chem. 2001;276:27462-9 pubmed
    ..These data demonstrate that ILK is an important upstream kinase for the regulation of PKB/Akt. ..
  37. Nikolopoulos S, Turner C. Integrin-linked kinase (ILK) binding to paxillin LD1 motif regulates ILK localization to focal adhesions. J Biol Chem. 2001;276:23499-505 pubmed
    ..Thus, paxillin binding is necessary for efficient focal adhesion targeting of ILK and may therefore impact the role of ILK in integrin-mediated signal transduction events. ..
  38. Sawai H, Okada Y, Funahashi H, Matsuo Y, Takahashi H, Takeyama H, et al. Integrin-linked kinase activity is associated with interleukin-1 alpha-induced progressive behavior of pancreatic cancer and poor patient survival. Oncogene. 2006;25:3237-46 pubmed
    ..Our results suggest that ILK is involved with aggressive capability in pancreatic cancer and that these regulations can be helpful to understand biological processes for a better translational treatment for pancreatic cancer patients. ..
  39. Kim Y, Choi S, Choi M, Oh M, Lee S, Cho M, et al. Cell adhesion-dependent cofilin serine 3 phosphorylation by the integrin-linked kinase.c-Src complex. J Biol Chem. 2008;283:10089-96 pubmed publisher
    ..Altogether, this study provides evidence for a new signaling connection from ILK to cofilin for dynamic actin polymerization during cell adhesion, depending on the activity of ILK-associated c-Src. ..
  40. Kiss E, Murányi A, Csortos C, Gergely P, Ito M, Hartshorne D, et al. Integrin-linked kinase phosphorylates the myosin phosphatase target subunit at the inhibitory site in platelet cytoskeleton. Biochem J. 2002;365:79-87 pubmed
    ..The above results raise the potential for regulation of MP activity in platelet cytoskeleton by ILK and suggest an alternative to the Rho-linked pathway. ..
  41. Guo L, Wu C. Regulation of fibronectin matrix deposition and cell proliferation by the PINCH-ILK-CH-ILKBP complex. FASEB J. 2002;16:1298-300 pubmed
  42. Zhang Y, Chen K, Guo L, Wu C. Characterization of PINCH-2, a new focal adhesion protein that regulates the PINCH-1-ILK interaction, cell spreading, and migration. J Biol Chem. 2002;277:38328-38 pubmed
    ..These results identify a novel nuclear and focal adhesion protein that associates with ILK and reveals an important role of PINCH-2 in the regulation of the PINCH-1-ILK interaction, cell shape change, and migration. ..
  43. Hashiramoto A, Murata M, Kawazoe T, Yoshida K, Akiyama C, Shiozawa K, et al. Heat shock protein 90 maintains the tumour-like character of rheumatoid synovial cells by stabilizing integrin-linked kinase, extracellular signal-regulated kinase and protein kinase B. Rheumatology (Oxford). 2011;50:852-61 pubmed publisher
    ..The contribution of HSP90 is important in the pathogenesis of RA that potentiates a tumour-like synovial overgrowth by stabilizing ILK, extracellular signal-regulated kinase and Akt. ..
  44. Cordes N, Blaese M, Meineke V, Van Beuningen D. Ionizing radiation induces up-regulation of functional beta1-integrin in human lung tumour cell lines in vitro. Int J Radiat Biol. 2002;78:347-57 pubmed
    ..Identification of such mechanisms will provide considerable insights into the understanding of tumorigenicity and metastatic phenotypes, possibly leading to new, optimized radiochemotherapeutic regimens. ..
  45. Oloumi A, Syam S, Dedhar S. Modulation of Wnt3a-mediated nuclear beta-catenin accumulation and activation by integrin-linked kinase in mammalian cells. Oncogene. 2006;25:7747-57 pubmed
    ..Finally, we suggest that a novel small molecule inhibitor of ILK, QLT-0267, may be a useful tool in the regulation of pathological Wnt signaling. ..
  46. Chung D, Lee J, Kook M, Kim J, Kim S, Choi E, et al. ILK (beta1-integrin-linked protein kinase): a novel immunohistochemical marker for Ewing's sarcoma and primitive neuroectodermal tumour. Virchows Arch. 1998;433:113-7 pubmed
    ..ILK may be a sensitive and specific immunohistochemical marker and useful for the positive identification of ES and PNET in formalin-fixed, paraffin-embedded tissue sections. ..
  47. Han Y, Yu Y, Nelson J, Wu C, Wang H, Michalopoulos G, et al. Interaction of integrin-linked kinase and miniature chromosome maintenance 7-mediating integrin {alpha}7 induced cell growth suppression. Cancer Res. 2010;70:4375-84 pubmed publisher
    ..We conclude that ILK interaction with MCM7 and MCM7 phosphorylation may be a critical event in ITGA7 signaling pathway, leading to tumor suppression. ..
  48. Fukuda T, Chen K, Shi X, Wu C. PINCH-1 is an obligate partner of integrin-linked kinase (ILK) functioning in cell shape modulation, motility, and survival. J Biol Chem. 2003;278:51324-33 pubmed
    ..These results provide new insights into the functions of PINCH proteins in regulation of ILK and alpha-parvin and control of cell behavior. ..
  49. Hannigan G, McDonald P, Walsh M, Dedhar S. Integrin-linked kinase: not so 'pseudo' after all. Oncogene. 2011;30:4375-85 pubmed publisher
    ..Here, we review evidence for and against ILK being an active kinase, and provide a framework for strategies to further analyze the kinase and adaptor functions of ILK in different cellular contexts. ..
  50. Pontier S, Huck L, White D, Rayment J, Sanguin Gendreau V, Hennessy B, et al. Integrin-linked kinase has a critical role in ErbB2 mammary tumor progression: implications for human breast cancer. Oncogene. 2010;29:3374-85 pubmed publisher
    ..These observations provide direct evidence that ILK has a critical role in the initiation phase of ErbB2 tumor induction. ..
  51. Mills J, Digicaylioglu M, Legg A, Young C, Young S, Barr A, et al. Role of integrin-linked kinase in nerve growth factor-stimulated neurite outgrowth. J Neurosci. 2003;23:1638-48 pubmed
    ..Consequently, ILK inhibition resulted in an increase in the hyperphosphorylation of Tau, a substrate of GSK-3. Together these findings indicate that ILK is an important effector in NGF-mediated neurite outgrowth. ..
  52. Kalra J, Warburton C, Fang K, Edwards L, Daynard T, Waterhouse D, et al. QLT0267, a small molecule inhibitor targeting integrin-linked kinase (ILK), and docetaxel can combine to produce synergistic interactions linked to enhanced cytotoxicity, reductions in P-AKT levels, altered F-actin architecture and improved treatment. Breast Cancer Res. 2009;11:R25 pubmed publisher
    ..The findings indicate that the 267/Dt drug combination confers increased (synergistic) therapeutic efficacy towards human breast cancer cells that express low levels of Her2. ..
  53. Fielding A, Dedhar S. The mitotic functions of integrin-linked kinase. Cancer Metastasis Rev. 2009;28:99-111 pubmed publisher
    ..In light of these findings, the therapeutic potential of the anti-mitotic effects of genetic or pharmacological inhibition of ILK will also be discussed. ..
  54. Zhang Y, Chen K, Tu Y, Wu C. Distinct roles of two structurally closely related focal adhesion proteins, alpha-parvins and beta-parvins, in regulation of cell morphology and survival. J Biol Chem. 2004;279:41695-705 pubmed
    ..Furthermore, they demonstrate that alpha- and beta-parvins play distinct roles in mammalian cells and suggest that the formation of the ILK-alpha-parvin complex is crucial for protection of cells from apoptosis. ..
  55. Dobreva I, Fielding A, Foster L, Dedhar S. Mapping the integrin-linked kinase interactome using SILAC. J Proteome Res. 2008;7:1740-9 pubmed publisher
  56. Zhao M, Gao Y, Wang L, Liu S, Han B, Ma L, et al. Overexpression of integrin-linked kinase promotes lung cancer cell migration and invasion via NF-?B-mediated upregulation of matrix metalloproteinase-9. Int J Med Sci. 2013;10:995-1002 pubmed publisher
    ..Taken together, these findings suggest that ILK promotes lung cancer cell migration and invasion via NF-?B-mediated upregulation of MMP-9. ..
  57. Zhu J, Pan X, Zhang Z, Gao J, Zhang L, Chen J. Downregulation of integrin-linked kinase inhibits epithelial-to-mesenchymal transition and metastasis in bladder cancer cells. Cell Signal. 2012;24:1323-32 pubmed
    ..Taken together, our findings highlight that ILK signaling pathway plays a novel role in the development of bladder cancer through regulating EMT. ILK could be a promising diagnostic marker and therapeutic target for bladder cancer. ..
  58. Nho R, Xia H, Kahm J, Kleidon J, Diebold D, Henke C. Role of integrin-linked kinase in regulating phosphorylation of Akt and fibroblast survival in type I collagen matrices through a beta1 integrin viability signaling pathway. J Biol Chem. 2005;280:26630-9 pubmed
    ..These data establish that matrix-derived mechanical forces sensed by beta1 integrin are capable of modulating ILK activity which regulates fibroblast viability via an Akt-dependent mechanism. ..
  59. Yamaji S, Suzuki A, Sugiyama Y, Koide Y, Yoshida M, Kanamori H, et al. A novel integrin-linked kinase-binding protein, affixin, is involved in the early stage of cell-substrate interaction. J Cell Biol. 2001;153:1251-64 pubmed
    ..The coexpression of ILK enhances this effect. These results provide evidence suggesting that affixin is involved in integrin-ILK signaling required for the establishment of cell-substrate adhesion. ..
  60. Chan J, Ko F, Yeung Y, Ng I, Yam J. Integrin-linked kinase overexpression and its oncogenic role in promoting tumorigenicity of hepatocellular carcinoma. PLoS ONE. 2011;6:e16984 pubmed publisher
    ..Here our findings also provide the first validation about the oncogenic capacity of ILK in vivo by suppressing its expression in HCC cells. The oncogenic role of ILK is implicated to be mediated by Akt pathway. ..
  61. Chiswell B, Zhang R, Murphy J, Boggon T, Calderwood D. The structural basis of integrin-linked kinase-PINCH interactions. Proc Natl Acad Sci U S A. 2008;105:20677-82 pubmed publisher
    ..These data provide an atomic-resolution description of a key interaction in the ILK-PINCH-parvin scaffolding complex. ..
  62. Albasri A, Al Ghamdi S, Fadhil W, Aleskandarany M, Liao Y, Jackson D, et al. Cten signals through integrin-linked kinase (ILK) and may promote metastasis in colorectal cancer. Oncogene. 2011;30:2997-3002 pubmed publisher
    ..002). We conclude that Cten expression is of prognostic significance in CRC, and we delineate a Cten-ILK pathway controlling cell motility and possibly promoting metastasis. ..
  63. Peroukides S, Bravou V, Varakis J, Alexopoulos A, Kalofonos H, Papadaki H. ILK overexpression in human hepatocellular carcinoma and liver cirrhosis correlates with activation of Akt. Oncol Rep. 2008;20:1337-44 pubmed
    ..Our results indicate that ILK overexpression during liver oncogenesis and cirrhosis correlates with activation of Akt but not with other conventional ILK targets. ..
  64. Intaraprasong P, Assi K, Owen D, Huntsman D, Chung S, Scudamore C, et al. Expression of integrin-linked kinase is not a useful prognostic marker in resected hepatocellular cancer. Anticancer Res. 2007;27:4371-6 pubmed
    ..0004), however, there was no direct correlation between the levels of expression of ILK with patient survival (log-rank test, p= 0.864). ILK expression does not appear to have a role in predicting outcome in patients with resected HCC. ..
  65. Hannigan G, Leung Hagesteijn C, Fitz Gibbon L, Coppolino M, Radeva G, Filmus J, et al. Regulation of cell adhesion and anchorage-dependent growth by a new beta 1-integrin-linked protein kinase. Nature. 1996;379:91-6 pubmed
    ..Endogenous ILK kinase activity was reduced in response to fibronectin. Overexpression of p59ILK disrupted epithelial cell architecture and inhibited adhesion to integrin substrates, while inducing anchorage-..
  66. Haase M, Gmach C, Eke I, Hehlgans S, Baretton G, Cordes N. Expression of integrin-linked kinase is increased in differentiated cells. J Histochem Cytochem. 2008;56:819-29 pubmed publisher
    ..This manuscript contains online supplemental material at http://www.jhc.org. Please visit this article online to view these materials. ..
  67. Bock Marquette I, Saxena A, White M, Dimaio J, Srivastava D. Thymosin beta4 activates integrin-linked kinase and promotes cardiac cell migration, survival and cardiac repair. Nature. 2004;432:466-72 pubmed
    ..These findings suggest that thymosin beta4 promotes cardiomyocyte migration, survival and repair and the pathway it regulates may be a new therapeutic target in the setting of acute myocardial damage. ..
  68. Acconcia F, Barnes C, Singh R, Talukder A, Kumar R. Phosphorylation-dependent regulation of nuclear localization and functions of integrin-linked kinase. Proc Natl Acad Sci U S A. 2007;104:6782-7 pubmed
    ..Together, these results suggest that ILK is a PAK1 substrate, undergoes phosphorylation-dependent shuttling between the cell nucleus and cytoplasm, and interacts with gene-regulatory chromatin. ..
  69. Mongroo P, Johnstone C, Naruszewicz I, Leung Hagesteijn C, Sung R, Carnio L, et al. Beta-parvin inhibits integrin-linked kinase signaling and is downregulated in breast cancer. Oncogene. 2004;23:8959-70 pubmed
    ..These results indicated that ParvB inhibits ILK signaling downstream of receptor tyrosine kinases. Our results suggest that loss of ParvB expression is a novel mechanism for upregulating ILK activity in tumors. ..