INCENP

Summary

Gene Symbol: INCENP
Description: inner centromere protein
Alias: inner centromere protein, binds and activates aurora-B and -C in vivo and in vitro, chromosomal passenger protein, inner centromere protein INCENP, inner centromere protein antigens 135/155kDa
Species: human
Products:     INCENP

Top Publications

  1. Gassmann R, Carvalho A, Henzing A, Ruchaud S, Hudson D, Honda R, et al. Borealin: a novel chromosomal passenger required for stability of the bipolar mitotic spindle. J Cell Biol. 2004;166:179-91 pubmed
    The chromosomal passenger complex of Aurora B kinase, INCENP, and Survivin has essential regulatory roles at centromeres and the central spindle in mitosis. Here, we describe Borealin, a novel member of the complex...
  2. Ainsztein A, Kandels Lewis S, Mackay A, Earnshaw W. INCENP centromere and spindle targeting: identification of essential conserved motifs and involvement of heterochromatin protein HP1. J Cell Biol. 1998;143:1763-74 pubmed
    The inner centromere protein (INCENP) has a modular organization, with domains required for chromosomal and cytoskeletal functions concentrated near the amino and carboxyl termini, respectively...
  3. Adams R, Maiato H, Earnshaw W, Carmena M. Essential roles of Drosophila inner centromere protein (INCENP) and aurora B in histone H3 phosphorylation, metaphase chromosome alignment, kinetochore disjunction, and chromosome segregation. J Cell Biol. 2001;153:865-80 pubmed
    ..RNA-mediated interference (RNAi) analysis of the role of two chromosomal passenger proteins, inner centromere protein (INCENP) and aurora B kinase, in cultured cells of Drosophila melanogaster...
  4. Zhu C, Bossy Wetzel E, Jiang W. Recruitment of MKLP1 to the spindle midzone/midbody by INCENP is essential for midbody formation and completion of cytokinesis in human cells. Biochem J. 2005;389:373-81 pubmed
    The INCENP (inner centromere protein) is a chromosomal passenger protein that plays multiple roles in regulating mitosis and cytokinesis...
  5. Klein U, Nigg E, Gruneberg U. Centromere targeting of the chromosomal passenger complex requires a ternary subcomplex of Borealin, Survivin, and the N-terminal domain of INCENP. Mol Biol Cell. 2006;17:2547-58 pubmed
    The chromosomal passenger complex (CPC), consisting of the serine/threonine kinase Aurora B, the inner centromere protein INCENP, Survivin, and Borealin/DasraB, has essential functions at the centromere in ensuring correct chromosome ..
  6. Earnshaw W, Cooke C. Analysis of the distribution of the INCENPs throughout mitosis reveals the existence of a pathway of structural changes in the chromosomes during metaphase and early events in cleavage furrow formation. J Cell Sci. 1991;98 ( Pt 4):443-61 pubmed
    ..This pathway is revealed by the existence of four distinct INCENP staining patterns in mitotic cells...
  7. Cutts S, Fowler K, Kile B, Hii L, O Dowd R, Hudson D, et al. Defective chromosome segregation, microtubule bundling and nuclear bridging in inner centromere protein gene (Incenp)-disrupted mice. Hum Mol Genet. 1999;8:1145-55 pubmed
    INCENP is a chromosomal passenger protein which relocates from the centromere to thel spindle midzone during the metaphase-anaphase transition, ultimately being discarded in the cell midbody at the completion of cytokinesis...
  8. Kaitna S, Mendoza M, Jantsch Plunger V, Glotzer M. Incenp and an aurora-like kinase form a complex essential for chromosome segregation and efficient completion of cytokinesis. Curr Biol. 2000;10:1172-81 pubmed
    In animal cells, cytokinesis begins shortly after the sister chromatids move to the spindle poles. The inner centromere protein (Incenp)has been implicated in both chromosome segregation and cytokinesis, but it is not known exactly how it ..
  9. Wheatley S, Kandels Lewis S, Adams R, Ainsztein A, Earnshaw W. INCENP binds directly to tubulin and requires dynamic microtubules to target to the cleavage furrow. Exp Cell Res. 2001;262:122-7 pubmed
    Inner centromere protein (INCENP) is a chromosomal passenger protein with an essential role in mitosis...

More Information

Publications104 found, 100 shown here

  1. Wheatley S, Carvalho A, Vagnarelli P, Earnshaw W. INCENP is required for proper targeting of Survivin to the centromeres and the anaphase spindle during mitosis. Curr Biol. 2001;11:886-90 pubmed
    ..lines of investigation have suggested that interactions between Survivin and the chromosomal passenger proteins INCENP and Aurora-B kinase may be important for mitotic progression...
  2. Vader G, Kauw J, Medema R, Lens S. Survivin mediates targeting of the chromosomal passenger complex to the centromere and midbody. EMBO Rep. 2006;7:85-92 pubmed
    ..complex (Aurora-B) is guided through the mitotic cell by its companion chromosomal passenger proteins, inner centromere protein (INCENP), Survivin and Borealin/Dasra-B, thereby allowing it to act at the right place at the right time...
  3. Jeyaprakash A, Klein U, Lindner D, Ebert J, Nigg E, Conti E. Structure of a Survivin-Borealin-INCENP core complex reveals how chromosomal passengers travel together. Cell. 2007;131:271-85 pubmed
    ..Survivin, Borealin, and INCENP are the three components of the CPC that regulate the activity and localization of its enzymatic component, the ..
  4. Martineau Thuillier S, Andreassen P, Margolis R. Colocalization of TD-60 and INCENP throughout G2 and mitosis: evidence for their possible interaction in signalling cytokinesis. Chromosoma. 1998;107:461-70 pubmed
    TD-60 and INCENP are two members of the chromosome passenger protein family, and each has been suggested to play a role in the control of cytokinesis...
  5. Honda R, Körner R, Nigg E. Exploring the functional interactions between Aurora B, INCENP, and survivin in mitosis. Mol Biol Cell. 2003;14:3325-41 pubmed
    ..Herein, we have investigated the regulation of human Aurora B by its complex partners inner centromere protein (INCENP) and survivin...
  6. Goto H, Kiyono T, Tomono Y, Kawajiri A, Urano T, Furukawa K, et al. Complex formation of Plk1 and INCENP required for metaphase-anaphase transition. Nat Cell Biol. 2006;8:180-7 pubmed
    ..Here, we report that Cdk1 phosphorylates Thr 59 and Thr 388 on inner centromere protein (INCENP), which regulates the localization and kinase activity of Aurora-B from prophase to metaphase...
  7. Uren A, Wong L, Pakusch M, Fowler K, Burrows F, Vaux D, et al. Survivin and the inner centromere protein INCENP show similar cell-cycle localization and gene knockout phenotype. Curr Biol. 2000;10:1319-28 pubmed
    ..5days post coitum. This phenotype, and the cell-cycle localization of Survivin, resembled closely those of INCENP. Because the yeast homolog of INCENP, Sli15, regulates the Aurora kinase homolog Ipl1p, and the yeast Survivin ..
  8. Kawashima S, Tsukahara T, Langegger M, Hauf S, Kitajima T, Watanabe Y. Shugoshin enables tension-generating attachment of kinetochores by loading Aurora to centromeres. Genes Dev. 2007;21:420-35 pubmed
    ..We propose that ensuring bipolar attachment of kinetochores is the primary role of shugoshin and the role of cohesion protection might have codeveloped to facilitate this process. ..
  9. Ling Y, Yuen K. Point centromere activity requires an optimal level of centromeric noncoding RNA. Proc Natl Acad Sci U S A. 2019;116:6270-6279 pubmed publisher
    ..CENP-ACse4, CENP-A chaperone HJURPScm3, CENP-CMif2, SurvivinBir1, and INCENPSli15; and a reduced chromatin localization of CENP-ACse4, CENP-CMif2, and Aurora ..
  10. García Rodríguez L, Kasciukovic T, Denninger V, Tanaka T. Aurora B-INCENP Localization at Centromeres/Inner Kinetochores Is Required for Chromosome Bi-orientation in Budding Yeast. Curr Biol. 2019;: pubmed publisher
    ..notion was challenged by the observation that Bir1 (yeast survivin), which recruits Ipl1-Sli15 (yeast Aurora B-INCENP) to centromeres, can become dispensable for bi-orientation [10]...
  11. Makrantoni V, Corbishley S, Rachidi N, Morrice N, Robinson D, Stark M. Phosphorylation of Sli15 by Ipl1 is important for proper CPC localization and chromosome stability in Saccharomyces cerevisiae. PLoS ONE. 2014;9:e89399 pubmed publisher
    ..of eukaryotic cell division, consisting of the protein kinase Aurora B/Ipl1 in association with its activator (INCENP/Sli15) and two additional proteins (Survivin/Bir1 and Borealin/Nbl1)...
  12. Carvalho A, Carmena M, Sambade C, Earnshaw W, Wheatley S. Survivin is required for stable checkpoint activation in taxol-treated HeLa cells. J Cell Sci. 2003;116:2987-98 pubmed
    Survivin is an essential chromosomal passenger protein whose function remains unclear. Here, we have used RNA interference to specifically repress Survivin in cultured HeLa cells...
  13. Temme A, Rieger M, Reber F, Lindemann D, Weigle B, Diestelkoetter Bachert P, et al. Localization, dynamics, and function of survivin revealed by expression of functional survivinDsRed fusion proteins in the living cell. Mol Biol Cell. 2003;14:78-92 pubmed
    ..These data characterize in detail the dynamics of survivin in vertebrate cells and confirm that survivin represents a chromosomal passenger protein.
  14. Terada Y. Aurora-B/AIM-1 regulates the dynamic behavior of HP1alpha at the G2-M transition. Mol Biol Cell. 2006;17:3232-41 pubmed
    ..In addition, depletion of INCENP led to aberrant cellular localization of Aurora-B/AIM-1, but it did not affect heterochromatin targeting of ..
  15. Sharif B, Na J, Lykke Hartmann K, McLaughlin S, Laue E, Glover D, et al. The chromosome passenger complex is required for fidelity of chromosome transmission and cytokinesis in meiosis of mouse oocytes. J Cell Sci. 2010;123:4292-300 pubmed publisher
    ..gain-of function approaches to assess the meiotic functions of one of the shared components of these complexes, INCENP, and of the variable kinase subunits, Aurora B or Aurora C...
  16. Vader G, Cruijsen C, van Harn T, Vromans M, Medema R, Lens S. The chromosomal passenger complex controls spindle checkpoint function independent from its role in correcting microtubule kinetochore interactions. Mol Biol Cell. 2007;18:4553-64 pubmed
    ..Replacing the chromosomal passenger protein INCENP with a mutant allele that lacks its coiled-coil domain results in an overt defect in a SAC-..
  17. Nakajima Y, Tyers R, Wong C, Yates J, Drubin D, Barnes G. Nbl1p: a Borealin/Dasra/CSC-1-like protein essential for Aurora/Ipl1 complex function and integrity in Saccharomyces cerevisiae. Mol Biol Cell. 2009;20:1772-84 pubmed publisher
    ..In Fungi and Animalia, this complex consists of the kinase Aurora B/AIR-2/Ipl1p, INCENP/ICP-1/Sli15p, and Survivin/BIR-1/Bir1p...
  18. Carmena M, Wheelock M, Funabiki H, Earnshaw W. The chromosomal passenger complex (CPC): from easy rider to the godfather of mitosis. Nat Rev Mol Cell Biol. 2012;13:789-803 pubmed publisher
    ..Aurora B is one of the most intensively studied kinases. In conjunction with inner centromere protein (INCENP), borealin (also known as Dasra) and survivin it forms the chromosomal passenger complex (CPC)...
  19. Labarrade F, Botto J, Domloge N. CRM1 and chromosomal passenger complex component survivin are essential to normal mitosis progress and to preserve keratinocytes from mitotic abnormalities. Int J Cosmet Sci. 2016;38:452-61 pubmed publisher
    ..The CPC is composed of four members: INCENP, survivin, borealin and aurora kinase B, and the disruption of the CPC during cell division induces mitotic ..
  20. Portella G, Passaro C, Chieffi P. Aurora B: a new prognostic marker and therapeutic target in cancer. Curr Med Chem. 2011;18:482-96 pubmed
    ..Aurora B is a chromosomal passenger protein involved in chromosome segregation, spindle-checkpoint, and cytokinesis...
  21. Gohard F, St Cyr D, Tyers M, Earnshaw W. Targeting the INCENP IN-box-Aurora B interaction to inhibit CPC activity in vivo. Open Biol. 2014;4:140163 pubmed publisher
    ..The CPC consists of Aurora B kinase, inner centromere protein (INCENP), and the targeting subunits survivin and borealin/Dasra B...
  22. Berry L, Chen C, Francia M, Guérin A, Graindorge A, Saliou J, et al. Toxoplasma gondii chromosomal passenger complex is essential for the organization of a functional mitotic spindle: a prerequisite for productive endodyogeny. Cell Mol Life Sci. 2018;75:4417-4443 pubmed publisher
    ..In higher Eukaryotes, the four-subunit chromosomal passenger complex (CPC) (Aurora kinase B (ARKB)/INCENP/Borealin/Survivin) promotes chromosome bi-orientation by detaching incorrect kinetochore-microtubule attachments, ..
  23. Elkins J, Santaguida S, Musacchio A, Knapp S. Crystal structure of human aurora B in complex with INCENP and VX-680. J Med Chem. 2012;55:7841-8 pubmed publisher
    ..the structure of the human Aurora B kinase domain in complex with the C-terminal Aurora-binding region of human INCENP and the Aurora kinase inhibitor VX-680...
  24. Silke J, Vaux D. IAP gene deletion and conditional knockout models. Semin Cell Dev Biol. 2015;39:97-105 pubmed publisher
    ..invertebrates and mammals has shown that it functions differently, as it is not a regulator of innate immunity or apoptosis, but acts together with INCENP, aurora kinase B and Borealin to allow chromosome segregation during mitosis.
  25. Xavier de Carvalho A, Maiato H, Maia A, Ribeiro S, Pontes P, Bickmore W, et al. Reed-Sternberg cells form by abscission failure in the presence of functional Aurora B kinase. PLoS ONE. 2015;10:e0124629 pubmed publisher
    ..mononucleated cells are chromosomally unstable, but this is unlikely to be related to a malfunctioning chromosomal passenger protein complex...
  26. Chu Y, Yao P, Wang W, Wang D, Wang Z, Zhang L, et al. Aurora B kinase activation requires survivin priming phosphorylation by PLK1. J Mol Cell Biol. 2011;3:260-7 pubmed publisher
    ..spindle microtubules to kinetochore requires the chromosomal passenger of Aurora B kinase complex with borealin, INCENP and survivin (SUR)...
  27. Fant X, Samejima K, Carvalho A, Ogawa H, Xu Z, Yue Z, et al. Use of DT40 conditional-knockout cell lines to study chromosomal passenger protein function. Biochem Soc Trans. 2010;38:1655-9 pubmed publisher
    The CPC [chromosomal passenger complex; INCENP (inner centromere protein), Aurora B kinase, survivin and borealin] is implicated in many mitotic processes...
  28. Carmena M, Pinson X, Platani M, Salloum Z, Xu Z, Clark A, et al. The chromosomal passenger complex activates Polo kinase at centromeres. PLoS Biol. 2012;10:e1001250 pubmed publisher
    ..Our study concerns the coordination of the activities of these two kinases in vivo. We show that INCENP, a key scaffolding subunit of the chromosomal passenger complex (CPC), which consists of Aurora B kinase, INCENP, ..
  29. Fellmeth J, Gordon D, Robins C, Scott R, Treff N, Schindler K. Expression and characterization of three Aurora kinase C splice variants found in human oocytes. Mol Hum Reprod. 2015;21:633-44 pubmed publisher
    ..By quantifying metaphase chromosome alignment, cell cycle progression, phosphorylation of INCENP and microtubule attachments to kinetochores, we found that AURKC_v1 was the most capable of the variants at ..
  30. Shimogawa M, Widlund P, Riffle M, Ess M, Davis T. Bir1 is required for the tension checkpoint. Mol Biol Cell. 2009;20:915-23 pubmed publisher
    The Saccharomyces cerevisiae chromosomal passenger proteins Ipl1 (Aurora B) and Sli15 (INCENP) are required for the tension checkpoint, but the role of the third passenger, Bir1, is controversial...
  31. Fernández Miranda G, Pérez de Castro I, Carmena M, Aguirre Portolés C, Ruchaud S, Fant X, et al. SUMOylation modulates the function of Aurora-B kinase. J Cell Sci. 2010;123:2823-33 pubmed publisher
    ..Aurora B can bind SUMO peptides in vitro when bound to the IN-box domain of its CPC partner INCENP. Mutation of Lys207 to arginine (Aurora B(K207R)) impairs the formation of conjugates of Aurora B and SUMO in vivo...
  32. Bakhrebah M, Zhang T, Mann J, Kalitsis P, Hudson D. Disruption of a conserved CAP-D3 threonine alters condensin loading on mitotic chromosomes leading to chromosome hypercondensation. J Biol Chem. 2015;290:6156-67 pubmed publisher
    ..it disrupts prophase, results in a dramatic shortening of the mitotic chromosome axis, and leads to abnormal INCENP localization...
  33. Xu Z, Vagnarelli P, Ogawa H, Samejima K, Earnshaw W. Gradient of increasing Aurora B kinase activity is required for cells to execute mitosis. J Biol Chem. 2010;285:40163-70 pubmed publisher
    ..INCENP (inner centromere protein) binds and activates Aurora B through a feedback loop involving phosphorylation of a Thr-Ser-Ser (TSS) ..
  34. Carmena M, Lombardía M, Ogawa H, Earnshaw W. Polo kinase regulates the localization and activity of the chromosomal passenger complex in meiosis and mitosis in Drosophila melanogaster. Open Biol. 2014;4:140162 pubmed publisher
    ..centromere in mitosis, and that the interaction between Polo and the chromosomal passenger complex (CPC) component INCENP is essential in this activation...
  35. Clijsters L, van Zon W, Riet B, Voets E, Boekhout M, Ogink J, et al. Inefficient degradation of cyclin B1 re-activates the spindle checkpoint right after sister chromatid disjunction. Cell Cycle. 2014;13:2370-8 pubmed publisher
    ..When cyclin B1 remained in late anaphase, cytokinesis stalled, and translocation of INCENP from separated sister chromatids to the spindle midzone was blocked...
  36. Cho Y, Lai C, Lin K, Hsu H. A Targeted RNAi Screen Reveals Drosophila Female-Sterile Genes That Control the Size of Germline Stem Cell Niche During Development. G3 (Bethesda). 2018;8:2345-2354 pubmed publisher
    ..1), a chromatin remodeling complex member (Enhancer of yellow 3) and a chromosome passenger complex constituent (Incenp) were identified as potentially functioning in the control of niche size...
  37. Joukov V, De Nicolo A. Aurora-PLK1 cascades as key signaling modules in the regulation of mitosis. Sci Signal. 2018;11: pubmed publisher
    ..kinases serve as catalytic subunits of distinct complexes formed with the four scaffold proteins Bora, CEP192, INCENP, and TPX2, which we deem "core" Aurora cofactors...
  38. Kichigin I, Lisachov A, Giovannotti M, Makunin A, Kabilov M, O Brien P, et al. First report on B chromosome content in a reptilian species: the case of Anolis carolinensis. Mol Genet Genomics. 2019;294:13-21 pubmed publisher
    ..to other supernumerary chromosomes from different taxons: they contain two genes related to cell division control (INCENP and SPIRE2), are enriched in specific repeats, and show a high degree of pseudogenization...
  39. Bohnert K, Chen J, Clifford D, Vander Kooi C, Gould K. A link between aurora kinase and Clp1/Cdc14 regulation uncovered by the identification of a fission yeast borealin-like protein. Mol Biol Cell. 2009;20:3646-59 pubmed publisher
    ..This complex is composed of a catalytic subunit, Aurora B kinase, and three nonenzymatic subunits, INCENP, Survivin, and Borealin...
  40. McKenzie C, Bassi Z, Debski J, Gottardo M, Callaini G, Dadlez M, et al. Cross-regulation between Aurora B and Citron kinase controls midbody architecture in cytokinesis. Open Biol. 2016;6: pubmed publisher
    ..In addition, we show that CIT-K promotes Aurora B activity through phosphorylation of the INCENP CPC subunit at the TSS motif...
  41. Liu J, Sun Y, Jiang Q, Yang C, Huang J, Li J, et al. [Functional haplotypes of INCENP affect promoter activity and bovine semen quality]. Yi Chuan. 2016;38:62-71 pubmed publisher
    To explore the association between single nucleotide polymorphisms (SNPs) in the promoter region of the inner centromere protein (INCENP) gene and bovine semen quality, the haplotypes in 250 Chinese Holstein bulls were detected using PCR-..
  42. Lee M, Lee E, Jin S, Ahn S, Kim S, Kim J, et al. Leptin regulates the pro-inflammatory response in human epidermal keratinocytes. Arch Dermatol Res. 2018;310:351-362 pubmed publisher
    ..of CENPA and CENPM was not coupled with those of other chromosome passenger complex like Aurora A kinase, INCENP, and survivin...
  43. Fink S, Turnbull K, Desai A, Campbell C. An engineered minimal chromosomal passenger complex reveals a role for INCENP/Sli15 spindle association in chromosome biorientation. J Cell Biol. 2017;216:911-923 pubmed publisher
    ..We engineered a minimal CPC comprised of the dimerized last third of the kinase-activating Sli15/INCENP scaffold and the catalytic subunit Ipl1/Aurora B...
  44. Samejima K, Platani M, Wolny M, Ogawa H, Vargiu G, Knight P, et al. The Inner Centromere Protein (INCENP) Coil Is a Single α-Helix (SAH) Domain That Binds Directly to Microtubules and Is Important for Chromosome Passenger Complex (CPC) Localization and Function in Mitosis. J Biol Chem. 2015;290:21460-72 pubmed publisher
    The chromosome passenger complex (CPC) is a master regulator of mitosis. Inner centromere protein (INCENP) acts as a scaffold regulating CPC localization and activity...
  45. Ghanizadeh Vesali S, Zekri A, Zaker F, Zaghal A, Yousefi M, Alimoghaddam K, et al. Significance of AZD1152 as a potential treatment against Aurora B overexpression in acute promyelocytic leukemia. Ann Hematol. 2016;95:1031-42 pubmed publisher
    Aurora B kinase as a chromosomal passenger protein plays multiple roles in regulating mitosis and cytokinesis. The function of Aurora B in leukemic cells has made it an important treatment target...
  46. Wheelock M, Wynne D, Tseng B, Funabiki H. Dual recognition of chromatin and microtubules by INCENP is important for mitotic progression. J Cell Biol. 2017;216:925-941 pubmed publisher
    The chromosomal passenger complex (CPC), composed of inner centromere protein (INCENP), Survivin, Borealin, and the kinase Aurora B, contributes to the activation of the mitotic checkpoint. The regulation of CPC function remains unclear...
  47. van der Horst A, Vromans M, Bouwman K, van der Waal M, Hadders M, Lens S. Inter-domain Cooperation in INCENP Promotes Aurora B Relocation from Centromeres to Microtubules. Cell Rep. 2015;12:380-7 pubmed publisher
    ..In budding yeast, this requires dephosphorylation of the microtubule-binding (MTB) domain of the INCENP analog Sli15. The mechanistic basis for this relocalization in metazoans is incompletely understood...
  48. Landino J, Ohi R. The Timing of Midzone Stabilization during Cytokinesis Depends on Myosin II Activity and an Interaction between INCENP and Actin. Curr Biol. 2016;26:698-706 pubmed publisher
    ..We further show that the CPC scaffolding protein INCENP (inner centromere protein) binds actin, an interaction that is important for cytokinesis and for midzone MT stabilization following ..
  49. Unsal E, Degirmenci B, Harmanda B, Erman B, Ozlu N. A small molecule identified through an in silico screen inhibits Aurora B-INCENP interaction. Chem Biol Drug Des. 2016;88:783-794 pubmed publisher
    ..We describe a new potential inhibitor that exclusively targets the interaction site of Aurora B and its activator INCENP. We performed a structure-based virtual screening and determined five potential candidates of 200 000 compounds, ..
  50. Doherty K, Meere M, Piiroinen P. A mathematical model of Aurora B activity in prophase and metaphase. Math Biosci. 2016;277:153-65 pubmed publisher
    Aurora B kinase is a protein that controls several processes in mitosis when it is found associated with INCENP, Survivin and Borealin in a complex known as the Chromosomal Passenger Complex...
  51. Das A, Shah S, Fan B, Paik D, DiSanto D, Hinman A, et al. Spindle Assembly and Chromosome Segregation Requires Central Spindle Proteins in Drosophila Oocytes. Genetics. 2016;202:61-75 pubmed publisher
    ..In this study we have conducted genetic screens to identify genes that interact with subito or the CPC component Incenp. In addition, the meiotic mutant phenotype for some of the genes identified in these screens were characterized...
  52. Rangasamy D, Berven L, Ridgway P, Tremethick D. Pericentric heterochromatin becomes enriched with H2A.Z during early mammalian development. EMBO J. 2003;22:1599-607 pubmed
    ..Z can directly interact with the pericentric heterochromatin binding protein INCENP. We propose that H2A...
  53. Fu J, Bian M, Liu J, Jiang Q, Zhang C. A single amino acid change converts Aurora-A into Aurora-B-like kinase in terms of partner specificity and cellular function. Proc Natl Acad Sci U S A. 2009;106:6939-44 pubmed publisher
    ..Furthermore, Aurora-A(G198N) formed a complex with the Aurora-B partners, INCENP and Survivin, and its localization depended on this interaction...
  54. Becker M, Stolz A, Ertych N, Bastians H. Centromere localization of INCENP-Aurora B is sufficient to support spindle checkpoint function. Cell Cycle. 2010;9:1360-72 pubmed
    During mitosis, the chromosomal passenger complex (CPC) comprising the Aurora B kinase, INCENP, survivin and borealin is essential for correcting non-bipolar chromosome attachments and for cytokinesis...
  55. Platica M, Ionescu A, Ivan E, Holland J, Mandeli J, Platica O. PAR, a protein involved in the cell cycle, is functionally related to chromosomal passenger proteins. Int J Oncol. 2011;38:777-85 pubmed publisher
    ..It also formed complexes with Aurora A, and with survivin, Aurora B and INCENP. In addition, PAR increased Aurora B kinase activity on histone H3...
  56. Kang J, Chaudhary J, Dong H, Kim S, Brautigam C, Yu H. Mitotic centromeric targeting of HP1 and its binding to Sgo1 are dispensable for sister-chromatid cohesion in human cells. Mol Biol Cell. 2011;22:1181-90 pubmed publisher
    ..Here we show that HP1α is targeted to mitotic centromeres by INCENP, a subunit of the chromosome passenger complex (CPC)...
  57. Quartuccio S, Dipali S, Schindler K. Haspin inhibition reveals functional differences of interchromatid axis-localized AURKB and AURKC. Mol Biol Cell. 2017;28:2233-2240 pubmed publisher
    ..cells contain a second chromosomal passenger complex (CPC) population, with Aurora kinase B (AURKB) bound to INCENP. Haspin inhibition in oocytes from Aurkc-/- mice, where AURKB is the sole ..
  58. Makrantoni V, Ciesiolka A, Lawless C, Fernius J, MARSTON A, Lydall D, et al. A Functional Link Between Bir1 and the Saccharomyces cerevisiae Ctf19 Kinetochore Complex Revealed Through Quantitative Fitness Analysis. G3 (Bethesda). 2017;7:3203-3215 pubmed publisher
    ..of eukaryotic cell division, consisting of the protein kinase Aurora B/Ipl1 in association with its activator (INCENP/Sli15) and two additional proteins (Survivin/Bir1 and Borealin/Nbl1)...
  59. Parra M, Viera A, Gómez R, Page J, Carmena M, Earnshaw W, et al. Dynamic relocalization of the chromosomal passenger complex proteins inner centromere protein (INCENP) and aurora-B kinase during male mouse meiosis. J Cell Sci. 2003;116:961-74 pubmed
    b>INCENP and aurora-B kinase are two chromosomal passenger proteins that are thought to play key roles in coordinating chromosome segregation with cytokinesis in somatic cells...
  60. Hans F, Skoufias D, Dimitrov S, Margolis R. Molecular distinctions between Aurora A and B: a single residue change transforms Aurora A into correctly localized and functional Aurora B. Mol Biol Cell. 2009;20:3491-502 pubmed publisher
    ..to mimic the aligned asparagine 142 (N142) of Aurora B, causes Aurora A to bind the Aurora B binding partner INCENP but not the Aurora A binding partner TPX2. The mutant Aurora A rescues Aurora B mitotic function...
  61. Maerki S, Olma M, Staubli T, Steigemann P, Gerlich D, Quadroni M, et al. The Cul3-KLHL21 E3 ubiquitin ligase targets aurora B to midzone microtubules in anaphase and is required for cytokinesis. J Cell Biol. 2009;187:791-800 pubmed publisher
    ..Together, our results suggest that different Cul3 adaptors nonredundantly regulate aurora B during mitosis, possibly by ubiquitinating different pools of aurora B at distinct subcellular localizations. ..
  62. Tsukahara T, Tanno Y, Watanabe Y. Phosphorylation of the CPC by Cdk1 promotes chromosome bi-orientation. Nature. 2010;467:719-23 pubmed publisher
    ..the chromosomal passenger complex (CPC), composed of catalytic kinase Aurora B and regulatory components (INCENP, Survivin and Borealin), must be localized to centromeres to phosphorylate kinetochore substrates...
  63. Deng X, von Keudell G, Suzuki T, Dohmae N, Nakakido M, Piao L, et al. PRMT1 promotes mitosis of cancer cells through arginine methylation of INCENP. Oncotarget. 2015;6:35173-82 pubmed publisher
    b>Inner centromere protein (INCENP) is a part of a protein complex known as the chromosomal passenger complex (CPC) that is essential for correcting non-bipolar chromosome attachments and for cytokinesis...
  64. Adams R, Eckley D, Vagnarelli P, Wheatley S, Gerloff D, Mackay A, et al. Human INCENP colocalizes with the Aurora-B/AIRK2 kinase on chromosomes and is overexpressed in tumour cells. Chromosoma. 2001;110:65-74 pubmed
    The inner centromere protein (INCENP), which has previously been described in chicken, frog and mouse, is required for correct chromosome segregation and cytokinesis...
  65. Ban R, Nishida T, Urano T. Mitotic kinase Aurora-B is regulated by SUMO-2/3 conjugation/deconjugation during mitosis. Genes Cells. 2011;16:652-69 pubmed publisher
    ..Based on these results, we propose that Aurora-B is a novel mitotic SUMO substrate and that its kinase activity is fine-tuned by the SUMO system. ..
  66. Kabisch M, Lorenzo Bermejo J, Dünnebier T, Ying S, Michailidou K, Bolla M, et al. Inherited variants in the inner centromere protein (INCENP) gene of the chromosomal passenger complex contribute to the susceptibility of ER-negative breast cancer. Carcinogenesis. 2015;36:256-71 pubmed publisher
    ..Fifteen SNPs in four CPC genes (INCENP, AURKB, BIRC5 and CDCA8) were genotyped in 88 911 European women from 39 case-control studies of the Breast Cancer ..
  67. Earnshaw W, Mackay A. Role of nonhistone proteins in the chromosomal events of mitosis. FASEB J. 1994;8:947-56 pubmed
    ..Thus, although recent advances appear to have taken us many steps closer to an understanding of the underlying mechanisms, we suspect that final answers will be some time in coming. ..
  68. Qi W, Tang Z, Yu H. Phosphorylation- and polo-box-dependent binding of Plk1 to Bub1 is required for the kinetochore localization of Plk1. Mol Biol Cell. 2006;17:3705-16 pubmed
    ..Our results suggest that phosphorylation of Bub1 at T609 by Cdk1 creates a docking site for the PBD of Plk1 and facilitates the kinetochore recruitment of Plk1. ..
  69. Knauer S, Bier C, Habtemichael N, Stauber R. The Survivin-Crm1 interaction is essential for chromosomal passenger complex localization and function. EMBO Rep. 2006;7:1259-65 pubmed
    The chromosomal passenger complex (CPC) of Aurora-B, Borealin, INCENP (inner centromere protein) and Survivin coordinates essential chromosomal and cytoskeletal events during mitosis...
  70. Katayama H, Sasai K, Kloc M, Brinkley B, Sen S. Aurora kinase-A regulates kinetochore/chromatin associated microtubule assembly in human cells. Cell Cycle. 2008;7:2691-704 pubmed
    ..of g-tubulin in the vicinity of kinetochore/chromatin to create microtubule nucleation sites as well as INCENP and TPX2 mediated activation of Aurora-A facilitating formation and stabilization of microtubules...
  71. Dunajová L, Cash E, Márkus R, Rochette S, Townley A, Wheatley S. The N-terminus of survivin is a mitochondrial-targeting sequence and Src regulator. J Cell Sci. 2016;129:2707-12 pubmed publisher
    ..and its localisation during mitosis to the centromeres and midzone microtubules is that of a canonical chromosomal passenger protein. In addition to these well-established locations, survivin is also a mitochondrial protein, but how it ..
  72. Barbanis S, Ioannou M, Kouvaras E, Karasavvidou F, Nakou M, Papamichali R, et al. INCENP (inner centromere protein) is overexpressed in high grade non-Hodgkin B-cell lymphomas. Pathol Oncol Res. 2009;15:11-7 pubmed publisher
    b>Inner centromere protein (INCENP) is a member of the Chromosomal Passenger Complex (CPC), which is a four member protein complex essential for proper completion of mitosis and cell division (cytokinesis)...
  73. Xu Z, Ogawa H, Vagnarelli P, Bergmann J, Hudson D, Ruchaud S, et al. INCENP-aurora B interactions modulate kinase activity and chromosome passenger complex localization. J Cell Biol. 2009;187:637-53 pubmed publisher
    ..CPC targeting to centromeres involves interactions between Survivin, Borealin, and the inner centromere protein (CENP [INCENP]) N terminus...
  74. Faitar S, Sossey Alaoui K, Ranalli T, Cowell J. EVI5 protein associates with the INCENP-aurora B kinase-survivin chromosomal passenger complex and is involved in the completion of cytokinesis. Exp Cell Res. 2006;312:2325-35 pubmed
    ..and GST pull-down approaches to demonstrate that EVI5 is associated with the aurora B kinase protein complex (INCENP, aurora B kinase and survivin)...
  75. Sun L, Gao J, Dong X, Liu M, Li D, Shi X, et al. EB1 promotes Aurora-B kinase activity through blocking its inactivation by protein phosphatase 2A. Proc Natl Acad Sci U S A. 2008;105:7153-8 pubmed publisher
    ..These findings establish Aurora-B as an EB1-interacting protein and suggest that EB1 stimulates Aurora-B activity through antagonizing its dephosphorylation/inactivation by PP2A. ..
  76. Tanneeru K, Guruprasad L. Structural basis for binding of aurora-AG198N- INCENP complex: MD simulations and free energy calculations. Protein Pept Lett. 2013;20:1246-56 pubmed
    ..activity, the mutation G198N transforms Aurora-A to Aurora-B like function and localization by binding to Inner centromere protein (INCENP)...
  77. Sasai K, Katayama H, Hawke D, Sen S. Aurora-C Interactions with Survivin and INCENP Reveal Shared and Distinct Features Compared with Aurora-B Chromosome Passenger Protein Complex. PLoS ONE. 2016;11:e0157305 pubmed publisher
    ..of Aurora-C interactions with members of the Chromosome Passenger Complex (CPC), Survivin and Inner Centromere Protein (INCENP) in reference to known Aurora-B interactions to understand the functional significance of Aurora-..
  78. Li X, Sakashita G, Matsuzaki H, Sugimoto K, Kimura K, Hanaoka F, et al. Direct association with inner centromere protein (INCENP) activates the novel chromosomal passenger protein, Aurora-C. J Biol Chem. 2004;279:47201-11 pubmed
    ..The present study shows that human Aurora-C is a chromosomal passenger protein that forms complexes with Aurora-B and inner centromere protein (INCENP), which are known passenger ..
  79. Capalbo L, Mela I, Abad M, Jeyaprakash A, Edwardson J, D Avino P. Coordinated regulation of the ESCRT-III component CHMP4C by the chromosomal passenger complex and centralspindlin during cytokinesis. Open Biol. 2016;6: pubmed
    The chromosomal passenger complex (CPC)-composed of Aurora B kinase, Borealin, Survivin and INCENP-surveys the fidelity of genome segregation throughout cell division...
  80. Wong L, Brettingham Moore K, Chan L, Quach J, Anderson M, Northrop E, et al. Centromere RNA is a key component for the assembly of nucleoproteins at the nucleolus and centromere. Genome Res. 2007;17:1146-60 pubmed
    ..Here, we demonstrate that centromeric alpha-satellite RNA and proteins CENPC1 and INCENP accumulate in the human interphase nucleolus in an RNA polymerase I-dependent manner...
  81. Gómez V, Sesé M, Santamaria A, Martinez J, Castellanos E, Soler M, et al. Regulation of aurora B kinase by the lipid raft protein flotillin-1. J Biol Chem. 2010;285:20683-90 pubmed publisher
    ..Flotillin-1 interacted with Aurora B directly through its SPFH domain in a complex distinct from the chromosomal passenger protein complex, and the two proteins co-purified in nuclear, non-raft fractions...
  82. Yamagishi Y, Honda T, Tanno Y, Watanabe Y. Two histone marks establish the inner centromere and chromosome bi-orientation. Science. 2010;330:239-43 pubmed publisher
    ..Haspin colocalizes with cohesin by associating with Pds5, whereas Bub1 localizes at kinetochores. Thus, the inner centromere is defined by intersection of two histone kinases. ..
  83. Vuoriluoto M, Laine L, Saviranta P, Pouwels J, Kallio M. Spatio-temporal composition of the mitotic Chromosomal Passenger Complex detected using in situ proximity ligation assay. Mol Oncol. 2011;5:105-11 pubmed publisher
    ..mitotic phases and after chemical treatments using is-PLA with antibodies against the core CPC subunits Aurora B, INCENP, Survivin and Borealin...
  84. Kar S, Beesley J, Amin Al Olama A, Michailidou K, Tyrer J, Kote Jarai Z, et al. Genome-Wide Meta-Analyses of Breast, Ovarian, and Prostate Cancer Association Studies Identify Multiple New Susceptibility Loci Shared by at Least Two Cancer Types. Cancer Discov. 2016;6:1052-67 pubmed publisher
    ..loci: three associated with susceptibility to all three cancers (rs17041869/2q13/BCL2L11; rs7937840/11q12/INCENP; rs1469713/19p13/GATAD2A), two breast and ovarian cancer risk loci (rs200182588/9q31/SMC2; rs8037137/15q26/RCCD1), ..
  85. Wheatley S, Henzing A, Dodson H, Khaled W, Earnshaw W. Aurora-B phosphorylation in vitro identifies a residue of survivin that is essential for its localization and binding to inner centromere protein (INCENP) in vivo. J Biol Chem. 2004;279:5655-60 pubmed
    The chromosomal passengers, aurora-B kinase, inner centromere protein (INCENP), and survivin, are essential proteins that have been implicated in the regulation of metaphase chromosome alignment, spindle checkpoint function, and ..
  86. Chen H, Tang C, Chen C, Tang T. Overexpression of an Aurora-C kinase-deficient mutant disrupts the Aurora-B/INCENP complex and induces polyploidy. J Biomed Sci. 2005;12:297-310 pubmed
    ..Together, our results showed that Aurora-C is a chromosomal passenger protein, which may serve as a key regulator in cell division.
  87. Huang H, Bailis J, Leverson J, Gómez E, Forsburg S, Hunter T. Suppressors of Bir1p (Survivin) identify roles for the chromosomal passenger protein Pic1p (INCENP) and the replication initiation factor Psf2p in chromosome segregation. Mol Cell Biol. 2005;25:9000-15 pubmed
    Fission yeast Bir1p/Cut17p/Pbh1p, the homolog of human Survivin, is a conserved chromosomal passenger protein that is required for cell division and cytokinesis...
  88. Jahnke U, Higginbottom K, Newland A, Cotter F, Allen P. Cell death in leukemia: passenger protein regulation by topoisomerase inhibitors. Biochem Biophys Res Commun. 2007;361:928-33 pubmed
    ..Aurora were also associated with Cdk1 and were shown to form complexes, which in pull down experiments, included INCENP. There was no evidence of Aurora B kinase suppression...
  89. Klein U, Haindl M, Nigg E, Muller S. RanBP2 and SENP3 function in a mitotic SUMO2/3 conjugation-deconjugation cycle on Borealin. Mol Biol Cell. 2009;20:410-8 pubmed publisher
    ..The CPC, which additionally comprises INCENP, Survivin, and Aurora B, regulates key mitotic events, including chromosome congression, the spindle assembly ..
  90. Shimura M, Toyoda Y, Iijima K, Kinomoto M, Tokunaga K, Yoda K, et al. Epigenetic displacement of HP1 from heterochromatin by HIV-1 Vpr causes premature sister chromatid separation. J Cell Biol. 2011;194:721-35 pubmed publisher
    ..Our study reveals for the first time centromere cohesion impairment resulting from epigenetic disruption of higher-order structures of heterochromatin by a viral pathogen. ..
  91. Soeda S, Nakayama Y, Honda T, Aoki A, Tamura N, Abe K, et al. v-Src causes delocalization of Mklp1, Aurora B, and INCENP from the spindle midzone during cytokinesis failure. Exp Cell Res. 2013;319:1382-97 pubmed publisher
    ..These results suggest that v-Src inhibits cytokinesis through the delocalization of Mklp1 and Aurora B from the spindle midzone, resulting in binucleation. ..
  92. Landino J, Norris S, Li M, Ballister E, Lampson M, Ohi R. Two mechanisms coordinate the recruitment of the chromosomal passenger complex to the plane of cell division. Mol Biol Cell. 2017;28:3634-3646 pubmed publisher
    ..We show here that direct actin binding, via the inner centromere protein (INCENP), enhances CPC enrichment at the equatorial cortex, thus acting in tandem with MKlp2...