Gene Symbol: IFT74
Description: intraflagellar transport 74
Alias: BBS20, CCDC2, CMG-1, CMG1, intraflagellar transport protein 74 homolog, capillary morphogenesis gene 1 protein, capillary morphogenesis protein 1, coiled-coil domain containing 2, coiled-coil domain-containing protein 2, intraflagellar transport 74 homolog
Species: human
Products:     IFT74

Top Publications

  1. Bell S, Mavila A, Salazar R, Bayless K, Kanagala S, Maxwell S, et al. Differential gene expression during capillary morphogenesis in 3D collagen matrices: regulated expression of genes involved in basement membrane matrix assembly, cell cycle progression, cellular differentiation and G-protein signaling. J Cell Sci. 2001;114:2755-73 pubmed
    ..These data further elucidate the genetic events regulating capillary tube formation in a 3D matrix environment. ..
  2. Wachter S, Jung J, Shafiq S, Basquin J, Fort C, Bastin P, et al. Binding of IFT22 to the intraflagellar transport complex is essential for flagellum assembly. EMBO J. 2019;38: pubmed publisher
    ..associates with G-nucleotides and present crystal structures of IFT22 in complex with GDP, GTP, and with IFT74/81. Our structural analysis unravels an unusual GTP/GDP-binding mode of IFT22 bypassing the classical G4 motif...
  3. Cevik S, Sanders A, Van Wijk E, Boldt K, Clarke L, van Reeuwijk J, et al. Active transport and diffusion barriers restrict Joubert Syndrome-associated ARL13B/ARL-13 to an Inv-like ciliary membrane subdomain. PLoS Genet. 2013;9:e1003977 pubmed publisher
    ..complex analysis of human ARL13B identified functional associations with IFT-B complexes, mapped to IFT46 and IFT74 interactions...
  4. Brown J, Cochran D, Craige B, Kubo T, Witman G. Assembly of IFT trains at the ciliary base depends on IFT74. Curr Biol. 2015;25:1583-93 pubmed publisher
    ..The IFT-B core proteins IFT74 and IFT81 interact directly through central and C-terminal coiled-coil domains, and recently it was shown that the ..
  5. Kubo T, Brown J, Bellve K, Craige B, Craft J, Fogarty K, et al. Together, the IFT81 and IFT74 N-termini form the main module for intraflagellar transport of tubulin. J Cell Sci. 2016;129:2106-19 pubmed publisher
    ..that, together, the calponin-homology domain within the IFT81 N-terminus and the highly basic N-terminus of IFT74 form a module for IFT of tubulin...
  6. Heininger A, Hackert P, Andreou A, Boon K, Memet I, Prior M, et al. Protein cofactor competition regulates the action of a multifunctional RNA helicase in different pathways. RNA Biol. 2016;13:320-30 pubmed publisher
    ..We identify the orphan G-patch protein Cmg1 (YLR271W) as a novel cofactor of Prp43 and show that it stimulates the RNA binding and ATPase activity of the ..
  7. Lindstrand A, Frangakis S, Carvalho C, Richardson E, McFadden K, Willer J, et al. Copy-Number Variation Contributes to the Mutational Load of Bardet-Biedl Syndrome. Am J Hum Genet. 2016;99:318-36 pubmed publisher
    ..Finally, we report a deletion and a splice site mutation in IFT74, inherited under a recessive paradigm, defining a candidate BBS locus...
  8. Nishijima Y, Hagiya Y, Kubo T, Takei R, Katoh Y, Nakayama K. RABL2 interacts with the intraflagellar transport-B complex and CEP19 and participates in ciliary assembly. Mol Biol Cell. 2017;28:1652-1666 pubmed publisher
    ..also show that RABL2 interacts, in its GTP-bound state, with the intraflagellar transport (IFT)-B complex via the IFT74-IFT81 heterodimer and that the interaction is disrupted by a mutation found in male infertile mice (Mot ..
  9. Bhogaraju S, Cajanek L, Fort C, Blisnick T, Weber K, Taschner M, et al. Molecular basis of tubulin transport within the cilium by IFT74 and IFT81. Science. 2013;341:1009-12 pubmed publisher
    ..However, the molecular mechanisms of IFT are poorly understood. Here, we found that the two core IFT proteins IFT74 and IFT81 form a tubulin-binding module and mapped the interaction to a calponin homology domain of IFT81 and a ..

More Information


  1. Kanie T, Abbott K, Mooney N, Plowey E, Demeter J, JACKSON P. The CEP19-RABL2 GTPase Complex Binds IFT-B to Initiate Intraflagellar Transport at the Ciliary Base. Dev Cell. 2017;42:22-36.e12 pubmed publisher
    ..Activated RABL2B then captures and releases its single effector, the intraflagellar transport B holocomplex, from the large pool of pre-docked IFT-B complexes, and thus initiates ciliary entry of IFT. ..
  2. Zhang Y, Liu H, Li W, Zhang Z, Shang X, Zhang D, et al. Intraflagellar transporter protein (IFT27), an IFT25 binding partner, is essential for male fertility and spermiogenesis in mice. Dev Biol. 2017;432:125-139 pubmed publisher
    ..and IFT81 were significantly reduced in the testis of the conditional Ift27 knockout mice, and levels of IFT20, IFT74, and IFT140 were not changed...
  3. Bhogaraju S, Weber K, Engel B, Lechtreck K, Lorentzen E. Getting tubulin to the tip of the cilium: one IFT train, many different tubulin cargo-binding sites?. Bioessays. 2014;36:463-7 pubmed publisher
    ..A recent study using purified IFT complexes has identified a tubulin-binding module in the two core IFT proteins IFT74 and IFT81 that likely serves to bind and transport tubulin within cilia...
  4. Xiao S, Sato C, Kawarai T, Goodall E, Pall H, Zinman L, et al. Genetic studies of GRN and IFT74 in amyotrophic lateral sclerosis. Neurobiol Aging. 2008;29:1279-82 pubmed
    ..We evaluated the majority of known genetic variability in IFT74 and GRN...
  5. Momeni P, Schymick J, Jain S, Cookson M, Cairns N, Greggio E, et al. Analysis of IFT74 as a candidate gene for chromosome 9p-linked ALS-FTD. BMC Neurol. 2006;6:44 pubmed
    ..locus revealed the presence of a disease segregating stop mutation (Q342X) in the intraflagellar transport 74 (IFT74) gene in family 476 (F476), but no mutation was detected within IFT74 in family 2 (F2)...
  6. Zhang W, Taylor S, Nevarez L, Lachman R, Nickerson D, Bamshad M, et al. IFT52 mutations destabilize anterograde complex assembly, disrupt ciliogenesis and result in short rib polydactyly syndrome. Hum Mol Genet. 2016;25:4012-4020 pubmed publisher
    ..IFT52 mutant cells synthesized a significantly reduced amount of IFT52 protein, leading to reduced synthesis of IFT74, IFT81, IFT88 and ARL13B, other key anterograde complex members...
  7. Priya S, Nampoothiri S, Sen P, Sripriya S. Bardet-Biedl syndrome: Genetics, molecular pathophysiology, and disease management. Indian J Ophthalmol. 2016;64:620-627 pubmed publisher
    ..The disease mapped to at least twenty different genes (BBS1-BBS20), follow oligogenic inheritance pattern...
  8. Iomini C, Tejada K, Mo W, Vaananen H, Piperno G. Primary cilia of human endothelial cells disassemble under laminar shear stress. J Cell Biol. 2004;164:811-7 pubmed
    ..Under LSS, all primary cilia disassembled, and centrosomes were deprived of CMG-1. We conclude that the exposure to LSS ends the IFT in cultured endothelial cells. ..