ICAT

Summary

Gene Symbol: ICAT
Description: catenin, beta interacting protein 1
Alias: ICAT, beta-catenin-interacting protein 1, beta-catenin-interacting protein ICAT, inhibitor of beta-catenin and Tcf-4
Species: human

Top Publications

  1. ncbi IQ-domain GTPase-activating protein 1 regulates beta-catenin at membrane ruffles and its role in macropinocytosis of N-cadherin and adenomatous polyposis coli
    Manisha Sharma
    Westmead Institute for Cancer Research, University of Sydney, Westmead Millennium Institute at Westmead Hospital, Westmead, New South Wales 2145, Australia
    J Biol Chem 282:8545-56. 2007
  2. ncbi Mascot file parsing and quantification (MFPaQ), a new software to parse, validate, and quantify proteomics data generated by ICAT and SILAC mass spectrometric analyses: application to the proteomics study of membrane proteins from primary human endothelia
    David Bouyssie
    Laboratoire de Protéomique et Spectrométrie de Masse des Biomolécules, Equipe Labellisée LIGUE 2006, Institut de Pharmacologie et de Biologie Structurale, CNRS UMR 5089, 205 route de Narbonne, 31077, Toulouse, France
    Mol Cell Proteomics 6:1621-37. 2007
  3. ncbi Identification of thioredoxin disulfide targets using a quantitative proteomics approach based on isotope-coded affinity tags
    Per Hägglund
    Enzyme and Protein Chemistry, Department of Systems Biology, Søltofts Plads, Building 224, Technical University of Denmark, DK 2800 Kgs Lyngby, Denmark
    J Proteome Res 7:5270-6. 2008
  4. doi Metadegradomics: toward in vivo quantitative degradomics of proteolytic post-translational modifications of the cancer proteome
    Alain Doucet
    Centre for Blood Research, 4 401 Life Sciences Institute, University of British Columbia, 2350 Health Sciences Mall, Vancouver, British Columbia V6T 1Z3, Canada
    Mol Cell Proteomics 7:1925-51. 2008
  5. ncbi Quantitative analysis of both protein expression and serine / threonine post-translational modifications through stable isotope labeling with dithiothreitol
    Keith Vosseller
    Department of Pharmaceutical Chemistry, University of California, San Francisco, CA 94143, USA
    Proteomics 5:388-98. 2005
  6. doi Quantitative proteomics analysis integrated with microarray data reveals that extracellular matrix proteins, catenins, and p53 binding protein 1 are important for chemotherapy response in ovarian cancers
    Sheng Pan
    Department of Pathology, University of Washington, Seattle, Washington, USA
    OMICS 13:345-54. 2009
  7. pmc Chronic ethanol feeding affects proteasome-interacting proteins
    Marie Pierre Bousquet-Dubouch
    CNRS, Institut de Pharmacologie et de Biologie Structurale, Toulouse, France
    Proteomics 9:3609-22. 2009
  8. ncbi [Effect of C/EBPalpha on the monocytic differentiation of HL60 cells induced by NSC67657]
    Wei Jia Wang
    Department of Laboratory Medicine, Zhongshan People s Hospital, The Affiliated Hospital of Zhongshan University, Zhongshan 528402, China
    Zhonghua Zhong Liu Za Zhi 32:262-6. 2010
  9. pmc Synergism between inositol phosphates and diacylglycerol on native TRPC6-like channels in rabbit portal vein myocytes
    A P Albert
    Department of Basic Medical Sciences, Pharmacology and Clinical Pharmacology, St George s Hospital Medical School, Cranmer Terrace, London SW17 0RE, UK
    J Physiol 552:789-95. 2003
  10. pmc Alpha 1-adrenoceptor activation of a non-selective cation current in rabbit portal vein by 1,2-diacyl-sn-glycerol
    R M Helliwell
    Department of Pharmacology and Clinical Pharmacology, St George s Hospital Medical School, London, UK
    J Physiol 499:417-28. 1997

Research Grants

Detail Information

Publications154 found, 100 shown here

  1. ncbi IQ-domain GTPase-activating protein 1 regulates beta-catenin at membrane ruffles and its role in macropinocytosis of N-cadherin and adenomatous polyposis coli
    Manisha Sharma
    Westmead Institute for Cancer Research, University of Sydney, Westmead Millennium Institute at Westmead Hospital, Westmead, New South Wales 2145, Australia
    J Biol Chem 282:8545-56. 2007
    ....
  2. ncbi Mascot file parsing and quantification (MFPaQ), a new software to parse, validate, and quantify proteomics data generated by ICAT and SILAC mass spectrometric analyses: application to the proteomics study of membrane proteins from primary human endothelia
    David Bouyssie
    Laboratoire de Protéomique et Spectrométrie de Masse des Biomolécules, Equipe Labellisée LIGUE 2006, Institut de Pharmacologie et de Biologie Structurale, CNRS UMR 5089, 205 route de Narbonne, 31077, Toulouse, France
    Mol Cell Proteomics 6:1621-37. 2007
    ..search engine and performs protein quantification in the case of isotopic labeling experiments using either the ICAT or SILAC (stable isotope labeling with amino acids in cell culture) method...
  3. ncbi Identification of thioredoxin disulfide targets using a quantitative proteomics approach based on isotope-coded affinity tags
    Per Hägglund
    Enzyme and Protein Chemistry, Department of Systems Biology, Søltofts Plads, Building 224, Technical University of Denmark, DK 2800 Kgs Lyngby, Denmark
    J Proteome Res 7:5270-6. 2008
    ..reduction based on thiol-specific differential labeling with the iodoacetamide-based isotope-coded affinity tag (ICAT) reagents...
  4. doi Metadegradomics: toward in vivo quantitative degradomics of proteolytic post-translational modifications of the cancer proteome
    Alain Doucet
    Centre for Blood Research, 4 401 Life Sciences Institute, University of British Columbia, 2350 Health Sciences Mall, Vancouver, British Columbia V6T 1Z3, Canada
    Mol Cell Proteomics 7:1925-51. 2008
    ..Quantitative proteomics using stable isotope labeling, such as ICAT, isobaric tags for relative and absolute quantification (iTRAQ), and stable isotope labeling by amino acids in cell ..
  5. ncbi Quantitative analysis of both protein expression and serine / threonine post-translational modifications through stable isotope labeling with dithiothreitol
    Keith Vosseller
    Department of Pharmaceutical Chemistry, University of California, San Francisco, CA 94143, USA
    Proteomics 5:388-98. 2005
    ..BEMAD was comparable to isotope-coded affinity tags (ICAT; a commercially available differential isotopic quantification technique) in protein expression analysis, but also ..
  6. doi Quantitative proteomics analysis integrated with microarray data reveals that extracellular matrix proteins, catenins, and p53 binding protein 1 are important for chemotherapy response in ovarian cancers
    Sheng Pan
    Department of Pathology, University of Washington, Seattle, Washington, USA
    OMICS 13:345-54. 2009
    ..We applied a quantitative proteomic approach based on ICAT/MS/MS technology to analyze tissues harvested at primary debulking surgery before the initiation of combination ..
  7. pmc Chronic ethanol feeding affects proteasome-interacting proteins
    Marie Pierre Bousquet-Dubouch
    CNRS, Institut de Pharmacologie et de Biologie Structurale, Toulouse, France
    Proteomics 9:3609-22. 2009
    ..b>ICAT and MS/MS spectral counting, further confirmed by Western blot, showed that the levels of several PIPs were ..
  8. ncbi [Effect of C/EBPalpha on the monocytic differentiation of HL60 cells induced by NSC67657]
    Wei Jia Wang
    Department of Laboratory Medicine, Zhongshan People s Hospital, The Affiliated Hospital of Zhongshan University, Zhongshan 528402, China
    Zhonghua Zhong Liu Za Zhi 32:262-6. 2010
    ..To figure out the function of C/EBPalpha in the monocytic differentiation of HL60 cells induced by a new steroidal drug NSC67657...
  9. pmc Synergism between inositol phosphates and diacylglycerol on native TRPC6-like channels in rabbit portal vein myocytes
    A P Albert
    Department of Basic Medical Sciences, Pharmacology and Clinical Pharmacology, St George s Hospital Medical School, Cranmer Terrace, London SW17 0RE, UK
    J Physiol 552:789-95. 2003
    In rabbit portal vein myocytes noradrenaline activates a non-selective cation current (Icat) which involves a transient receptor potential protein (TRPC6)...
  10. pmc Alpha 1-adrenoceptor activation of a non-selective cation current in rabbit portal vein by 1,2-diacyl-sn-glycerol
    R M Helliwell
    Department of Pharmacology and Clinical Pharmacology, St George s Hospital Medical School, London, UK
    J Physiol 499:417-28. 1997
    ..transduction mechanisms involved in the activation and modulation of the noradrenaline-activated cation current (Icat) were investigated with whole-cell patch clamp techniques in rabbit portal vein smooth muscle cells. 2...
  11. ncbi ANIBAL, stable isotope-based quantitative proteomics by aniline and benzoic acid labeling of amino and carboxylic groups
    Alexandre Panchaud
    Département de Microbiologie Fondamentale, Universite de Lausanne, Quartier UNIL Sorge, Bâtiment Biophore, CH 1015 Lausanne, Switzerland
    Mol Cell Proteomics 7:800-12. 2008
    ..Although the combination of existing approaches, e.g. ICAT with isotope-coded protein labeling, is analytically feasible, it implies high costs, and the combined application ..
  12. pmc Differential profiling of breast cancer plasma proteome by isotope-coded affinity tagging method reveals biotinidase as a breast cancer biomarker
    Un Beom Kang
    Life Sciences Division, Korea Institute of Science and Technology, Seoul 136 791, Korea
    BMC Cancer 10:114. 2010
    ..It is important to discover a reliable biomarker for the detection of breast cancer. Plasma is the most ideal source for cancer biomarker discovery since many cells cross-communicate through the secretion of soluble proteins into blood...
  13. doi Quantitative proteomics for identification of cancer biomarkers
    Raghothama Chaerkady
    Institute of Bioinformatics, International Technology Park, Bangalore, India Departments of Biological Chemistry, Pathology and Oncology, McKusick Nathans Institute of Genetic Medicine, Johns Hopkins University, Baltimore, MD, USA
    Proteomics Clin Appl 1:1080-9. 2007
    ..methods such as stable isotope labeling with amino acids in cell culture (SILAC), isotope-coded affinity tagging (ICAT), isobaric tags for relative and absolute quantitation (iTRAQ), and (18) O labeling have all been used in ..
  14. ncbi A method to identify and simultaneously determine the relative quantities of proteins isolated by gel electrophoresis
    Salvatore Sechi
    National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, 6707 Democracy Blvd, Rm 611, Bethesda, MD 20892 5460, USA
    Rapid Commun Mass Spectrom 16:1416-24. 2002
    ..g. using ICAT reagents or growing cells in isotopically enriched nutrients)...
  15. pmc Proteomic analysis of nipple aspirate fluid from women with early-stage breast cancer using isotope-coded affinity tags and tandem mass spectrometry reveals differential expression of vitamin D binding protein
    Timothy M Pawlik
    Department of Surgical Oncology, The University of Texas M, d, Anderson Cancer Center, Houston, Texas, USA
    BMC Cancer 6:68. 2006
    Isotope-coded affinity tag (ICAT) tandem mass spectrometry (MS) allows for qualitative and quantitative analysis of paired protein samples...
  16. ncbi Protease degradomics: mass spectrometry discovery of protease substrates and the CLIP-CHIP, a dedicated DNA microarray of all human proteases and inhibitors
    Christopher M Overall
    Department of Biochemistry and Molecular Biology, University of British Columbia, Vancouver, British Columbia V6T 1Z3, Canada
    Biol Chem 385:493-504. 2004
    ..In another approach we used isotope-coded affinity tag (ICAT) labeling with tandem mass spectrometry to quantitate the levels of secreted or shed extracellular proteins in MDA-..
  17. pmc Elucidation of thioredoxin target protein networks in mouse
    Cexiong Fu
    Center for Advanced Proteomics Research, Department of Biochemistry and Molecular Biology, University of Medicine and Dentistry of New Jersey, New Jersey Medical School Cancer Center, Newark, New Jersey 07103, USA
    Mol Cell Proteomics 8:1674-87. 2009
    ..In this study, we performed an ICAT proteomics study for the identification of Trx1 target proteins from the hearts of a cardiac specific Trx1-..
  18. ncbi Interagency relationships among rural early intervention collaboratives
    B J Polivka
    Ohio State University College of Nursing, Columbus, 43210, USA
    Public Health Nurs 18:340-9. 2001
    ..Respondents from 42 agencies completed the 18-item Interagency Collaboration Assessment Tool (ICAT) and demographic questions. The ICAT uses a 5-point Likert-type scale to assess the five domains of the model...
  19. ncbi Angiotensin II signaling pathways mediate expression of cardiac T-type calcium channels
    Laurent Ferron
    CNRS UMR 8078, Remodelage Tissulaire et Fonctionnel, Hopital Marie Lannelongue, 133 avenue de la Resistance, 92350 Le Plessis Robinson, France
    Circ Res 93:1241-8. 2003
    Recent studies indicate that cardiac T-type Ca2+ current (ICaT) reappears in hypertrophied ventricular cells...
  20. doi A mechanism-based ICAT strategy for comparing relative expression and activity levels of glycosidases in biological systems
    Omid Hekmat
    Department of Chemistry, University of British Columbia, Vancouver, BC, Canada
    J Proteome Res 7:3282-92. 2008
    An activity-based isotope-coded affinity tagging (AB-ICAT) strategy for proteome-wide quantitation of active retaining endoglycosidases has been developed...
  21. doi Apparent autosomal dominant keratoconus in a large Australian pedigree accounted for by digenic inheritance of two novel loci
    Kathryn P Burdon
    Department of Ophthalmology, Flinders University, Flinders Medical Centre, Bedford Park, Adelaide, SA, 5042, Australia
    Hum Genet 124:379-86. 2008
    ..Although the pedigree appears to demonstrate simple autosomal dominant inheritance, the disorder is actually genetically complex. This pedigree may provide a link between inherited forms of keratoconus and sporadic cases...
  22. doi Differential proteomics in the aging Noble rat ventral prostate
    Ying Wai Lam
    Department of Environmental Health, Division of Environmental Genetics and Molecular Toxicology, University of Cincinnati College of Medicine, Cincinnati, OH, USA
    Proteomics 8:2750-63. 2008
    ..of Noble rats by identifying differentially expressed prostate proteins between 3- and 16-month-old animals using ICAT and MS...
  23. pmc Comparative studies of early liver dysfunction in senescence-accelerated mouse using mitochondrial proteomics approaches
    Yashu Liu
    The National Laboratory of Protein Engineering and Plant Genetic Engineering, College of Life Sciences, Peking University, Beijing 100871, China
    Mol Cell Proteomics 7:1737-47. 2008
    ..Here we used both two-dimensional electrophoresis- and ICAT-based mitochondrial proteomics analysis to view the liver mitochondrial protein alterations between SAMP8 and SAMR1...
  24. pmc Pharmacoproteomics of a metalloproteinase hydroxamate inhibitor in breast cancer cells: dynamics of membrane type 1 matrix metalloproteinase-mediated membrane protein shedding
    Georgina S Butler
    Department of Oral Biological and Medical Sciences, Centre for Blood Research, University of British Columbia, Vancouver, Canada
    Mol Cell Biol 28:4896-914. 2008
    ..and membrane fractions with levels that were modulated by the MMPI were identified by isotope-coded affinity tag (ICAT) labeling and tandem mass spectrometry...
  25. ncbi Potentiating actions of lanthanum on ACh-induced cation current in guinea-pig ileal smooth muscle cells
    R Inoue
    Department of Pharmacology, Faculty of Medicine, Kyushu University, Fukuoka, Japan
    J Smooth Muscle Res 34:69-81. 1998
    Potentiating actions of external lanthanum (La3+) on muscarinic receptor-activated nonselective cation current (Icat) were investigated in myocytes dissociated from the longitudinal muscle layer of guinea-pig ileum, with a whole-cell ..
  26. pmc Beta-catenin binds to the activation function 2 region of the androgen receptor and modulates the effects of the N-terminal domain and TIF2 on ligand-dependent transcription
    Liang Nian Song
    Department of Oncology, Lombardi Cancer Center, Georgetown University School of Medicine, Washington, D C 20007 2197, USA
    Mol Cell Biol 23:1674-87. 2003
    ..b>ICAT, an inhibitor of T-cell factor 4 (TCF-4), and E-cadherin binding to beta-catenin also blocked binding of the ..
  27. ncbi Functional Ca2+ and Na+ channels on mouse Schwann cells cultured in serum-free medium: regulation by a diffusible factor from neurons and by cAMP
    C Beaudu-Lange
    INSERM U394, I F R 8, Bordeaux, France
    Eur J Neurosci 10:1796-809. 1998
    ..Of the Schwann cells that did not contact axons, 46.5% expressed T-type Ca2+ conductances (ICaT)...
  28. ncbi A comparative evaluation of software for the analysis of liquid chromatography-tandem mass spectrometry data from isotope coded affinity tag experiments
    Robert Moulder
    Turku Centre for Biotechnology, University of Turku and Abo Akademi University, 20521 Turku, Finland
    Proteomics 5:2748-60. 2005
    The options available for processing quantitative data from isotope coded affinity tag (ICAT) experiments have mostly been confined to software specific to the instrument of acquisition...
  29. ncbi Comprehensive proteomic analysis of protein changes during platelet storage requires complementary proteomic approaches
    Jonathan N Thon
    Department of Biochemistry and Molecular Biology, University of British Columbia, Vancouver, British Columbia, Canada
    Transfusion 48:425-35. 2008
    ..This study addresses relative differences among proteomics approaches to the analysis of the PLT storage lesion...
  30. ncbi In vivo drug-response in patients with leukemic non-Hodgkin's lymphomas is associated with in vitro chemosensitivity and gene expression profiling
    Kai Uwe Chow
    University Hospital, Department of Internal Medicine II, Hematology and Oncology, Frankfurt Main, Germany
    Pharmacol Res 53:49-61. 2006
    ..and cell cycle signalling including complement lysis inhibitor (clusterin/CLU), beta-catenin interacting protein (ICAT), peroxisome proliferator activated receptor alpha (PPARalpha), TNF alpha converting enzyme (ADAM17/TACE), homeo ..
  31. pmc MaXIC-Q Web: a fully automated web service using statistical and computational methods for protein quantitation based on stable isotope labeling and LC-MS
    Chih Chiang Tsou
    Institute of Information Science and Institute of Chemistry, Academia Sinica, Nankang, Taipei, Taiwan 115
    Nucleic Acids Res 37:W661-9. 2009
    ..g. SILAC, ICAT and user-developed labeling methods...
  32. ncbi [Quantitative analysis of nuclear proteome in apoptosis hepatoma cells induced by hydroxycamptothecin]
    Yu Rong Yan
    Chongqing University of Medical Science, Key Laboratory of Laboratory Medical Diagnostics, Ministry of Education, Chongqing 400016
    Fen Zi Xi Bao Sheng Wu Xue Bao 41:120-8. 2008
    ..Nuclear proteins labelled with cleavable isotope-coded affinity tag (c-ICAT) reagent were digested and purified...
  33. ncbi [17 beta-estradiol inhibits T-type calcium channels in mouse spermatogenic cells]
    Liang Lu
    The Ministry of Education Key Lab of Modern Toxicology, Nanjing Medical University, Nanjing, Jiangsu 210029, China
    Zhonghua Nan Ke Xue 14:483-8. 2008
    To investigate the effect of 17 beta-estradiol (E2) on T-type calcium currents (ICaT) in spermatogenic cells.
  34. doi An online lifestyle diary with a persuasive computer assistant providing feedback on self-management
    Olivier A Blanson Henkemans
    Delft University of Technology, Delft, The Netherlands
    Technol Health Care 17:253-67. 2009
    ..e., the use of an online lifestyle diary called DieetInzicht.nl. The assistant is represented by an animated iCat, which shows different facial expressions and provides cooperative feedback following principles from the ..
  35. doi Automated generic analysis tools for protein quantitation using stable isotope labeling
    Wen Lian Hsu
    Institute of Information Science, Academia Sinica, Taipei, Taiwan
    Methods Mol Biol 604:257-72. 2010
    ..g., iTRAQ-labeling quantitation, and the other by MS scans, e.g., quantitation using SILAC, ICAT, or (18)O labeling...
  36. ncbi The study of macromolecular complexes by quantitative proteomics
    Jeffrey A Ranish
    Institute for Systems Biology, 1441 North 34th Street, Seattle, Washington 98103 8904, USA
    Nat Genet 33:349-55. 2003
    ..It is based on the use of isotope-coded affinity tag (ICAT) reagents and mass spectrometry to compare the relative abundances of tryptic peptides derived from suitable pairs ..
  37. pmc Chromatin-specific regulation of LEF-1-beta-catenin transcription activation and inhibition in vitro
    A V Tutter
    Regulatory Biology Laboratory, The Salk Institute for Biological Studies, La Jolla, California 92037, USA
    Genes Dev 15:3342-54. 2001
    ..LEF-1-beta-cat transactivation in vitro was also repressed by inhibitor of beta-catenin and Tcf-4 (ICAT), a physiological inhibitor of Wnt/Wg signaling that interacts with ARM repeats 11 and 12, and by the nonsteroidal ..
  38. pmc Proteomics analysis of the expression of neurogranin in murine neuroblastoma (Neuro-2a) cells reveals its involvement for cell differentiation
    Nian Lin Reena Han
    Department of Biological Sciences, National University of Singapore, Singapore 117543, Singapore
    Int J Biol Sci 3:263-73. 2007
    ..changes due to the expression of Ng in murine neuroblastoma (Neuro-2a) cells using isotope coded affinity tags (ICAT) combined with 2-dimensional liquid chromatography/tandem mass spectrometry (2D-LC/MS/MS)...
  39. ncbi Methods for proteomics in neuroscience
    Nilesh S Tannu
    Department of Physiology and Pharmacology, Wake Forest University School of Medicine, Winston Salem, NC 27157, USA
    Prog Brain Res 158:41-82. 2006
    ..g., two-dimensional difference in gel electrophoresis (2D-DIGE), isotope-coded affinity tag (ICAT) and iTRAQ) are elaborated...
  40. pmc Quantification of oxidative posttranslational modifications of cysteine thiols of p21ras associated with redox modulation of activity using isotope-coded affinity tags and mass spectrometry
    Mahadevan Sethuraman
    Vascular Biology Unit, Boston University School of Medicine, Boston, MA 02118, USA
    Free Radic Biol Med 42:823-9. 2007
    ..Here we used an isotope-coded affinity tag (ICAT) and mass spectrometry to quantitate the reversible and irreversible oxidative posttranslational thiol ..
  41. doi Accuracy of reconstructed images from cone-beam computed tomography scans
    Manish Lamichane
    Department of Developmental Biology, School of Dental Medicine, Harvard University, Boston, Mass
    Am J Orthod Dentofacial Orthop 136:156.e1-6; discussion 156-7. 2009
    ..this study was to determine whether 2-dimensional (2D) images produced from cone-beam computed tomography (CBCT) images taken with an iCAT scanner (Imaging Sciences International, Hatfield, Pa) can substitute for traditional cephalograms.
  42. ncbi Genomic and proteomic expression analysis of Leishmania promastigote and amastigote life stages: the Leishmania genome is constitutively expressed
    Kirk Leifso
    Immunity and Infection Research Centre, Vancouver Coastal Health Research Institute, Canada
    Mol Biochem Parasitol 152:35-46. 2007
    ..Quantitative proteomics, using the isotope coded affinity tag (ICAT) technology and mass spectrometry, was used to identify L...
  43. pmc Proteomic profiling of lipopolysaccharide-activated macrophages by isotope coded affinity tagging
    Kristian E Swearingen
    Department of Chemistry, University of Washington, P O Box 351700, Seattle, Washington 98195 1700, USA
    J Proteome Res 9:2412-21. 2010
    ..We performed isotope coded affinity tagging (ICAT), multidimensional liquid chromatography, and mass spectrometry to identify proteins that are differently expressed ..
  44. doi A randomized controlled comparison of integrative cognitive-affective therapy (ICAT) and enhanced cognitive-behavioral therapy (CBT-E) for bulimia nervosa
    S A Wonderlich
    Neuropsychiatric Research Institute Department of Clinical Neuroscience, University of North Dakota School of Medicine and Health Sciences, Fargo, ND, USA
    Psychol Med 44:543-53. 2014
    The purpose of this investigation was to compare a new psychotherapy for bulimia nervosa (BN), integrative cognitive-affective therapy (ICAT), with an established treatment, 'enhanced' cognitive-behavioral therapy (CBT-E).
  45. ncbi Systematic uncovering of multiple pathways underlying the pathology of Huntington disease by an acid-cleavable isotope-coded affinity tag approach
    Ming Chang Chiang
    Institute of Biomedical Sciences, Academia Sinica, Taipei 11529, Taiwan
    Mol Cell Proteomics 6:781-97. 2007
    ..To identify proteins critical for the progression of HD, we applied acid-cleavable ICAT technology to quantitatively determine changes in protein expressions in the striatum of a transgenic HD mouse ..
  46. doi Redox proteomics of thiol proteins in mouse heart during ischemia/reperfusion using ICAT reagents and mass spectrometry
    Vikas Kumar
    Department of Pathology, University of Otago Christchurch, Christchurch 8140, New Zealand
    Free Radic Biol Med 58:109-17. 2013
    ..We have carried out an exploratory study using mass spectrometry with isotope-coded affinity tags (ICAT) aimed at identifying reversible oxidative changes to protein thiols in Langendorff perfused isolated mouse hearts ..
  47. doi Numerical models based on a minimal set of sarcolemmal electrogenic proteins and an intracellular Ca(2+) clock generate robust, flexible, and energy-efficient cardiac pacemaking
    Victor A Maltsev
    Laboratory of Cardiovascular Science, NIA, NIH, 251 Bayview Blvd, Baltimore, MD 21224, USA
    J Mol Cell Cardiol 59:181-95. 2013
    ..Inclusion of If or ICaT decreased the flexibility, but increased the robustness of the models (a relatively larger number of sets did not ..
  48. ncbi A comparative proteomic strategy for subcellular proteome research: ICAT approach coupled with bioinformatics prediction to ascertain rat liver mitochondrial proteins and indication of mitochondrial localization for catalase
    Xiao Sheng Jiang
    Research Centre for Proteome Analysis, Key Lab of Proteomics, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Graduate School of the Chinese Academy of Sciences, Shanghai 200031, China
    Mol Cell Proteomics 4:12-34. 2005
    ..To solve such a problem, we applied a high-throughput comparative proteome experimental strategy, ICAT approach performed with two-dimensional LC-MS/MS analysis, coupled with combinational usage of different ..
  49. ncbi Proteomic analysis of rat striatal synaptosomes during acrylamide intoxication at a low dose rate
    David S Barber
    Center for Environmental and Human Toxicology, University of Florida, Building 471, Mowry Road, Gainesville, Florida 32611 0885, USA
    Toxicol Sci 100:156-67. 2007
    ..of ACR on the cysteine-containing proteome of nerve terminal, we employed cleavable isotope-coded affinity tagging (ICAT) and liquid chromatography-tandem mass spectrometry...
  50. doi High-resolution two-dimensional electrophoresis
    Walter Weiss
    Technische Universitat Munchen, Fachgebiet Proteomik, Am Forum 2, D 85350 Freising Weihenstephan, Germany
    Methods Mol Biol 564:13-32. 2009
    ..Although alternative/complementary technologies, such as MudPIT, ICAT, or protein arrays, have emerged recently, there is up to now no technology that matches 2-DE in its ability for ..
  51. pmc Inhibition of beta-catenin signaling in articular chondrocytes results in articular cartilage destruction
    Mei Zhu
    University of Rochester, Rochester, New York 14642, USA
    Arthritis Rheum 58:2053-64. 2008
    ..To determine the role of beta-catenin signaling in articular chondrocyte function, we generated Col2a1-ICAT-transgenic mice to inhibit beta-catenin signaling in chondrocytes.
  52. pmc Applications of proteomics in the study of inflammatory bowel diseases: Current status and future directions with available technologies
    Philip Alex
    Division of Gastroenterology, Department of Medicine, Johns Hopkins University, Baltimore, Maryland 21205, USA
    Inflamm Bowel Dis 15:616-29. 2009
    ..These technologies include various mass spectrometry technologies, quantitative proteomics (2D-PAGE, ICAT, SILAC, iTRAQ), protein/antibody arrays, and multi-epitope-ligand cartography...
  53. doi Immunochromatographic assay on thread
    Gina Zhou
    Biomedical Engineering Department, McGill University, Montreal, QC, Canada
    Anal Chem 84:7736-43. 2012
    ..Here, we introduce the immunochromatographic assay on thread (ICAT) in a cartridge format that is suitable for multiplexing...
  54. pmc The beta-catenin binding protein ICAT modulates androgen receptor activity
    Ming Zhuo
    Department of Urology, Stanford University School of Medicine, Stanford, California 94305 5328, USA
    Mol Endocrinol 25:1677-88. 2011
    ..Here, we investigated inhibitor of β-catenin and T-cell factor (ICAT), a β-catenin binding protein that inhibits the canonical Wnt/β-catenin signaling pathway, in AR signaling...
  55. pmc β-Catenin determines upper airway progenitor cell fate and preinvasive squamous lung cancer progression by modulating epithelial-mesenchymal transition
    Adam Giangreco
    Centre for Respiratory Research, University College London, UK
    J Pathol 226:575-87. 2012
    ..Overall, the data show that airway basal cell β-catenin determines cell fate and its mis-expression is associated with the development of human lung cancer...
  56. doi Quantitative and temporal proteome analysis of butyrate-treated colorectal cancer cells
    Hwee Tong Tan
    Department of Biochemistry, Yong Loo Lin School of Medicine, National University of Singapore, 10 Kent Ridge Crescent, Singapore 117597, Singapore
    Mol Cell Proteomics 7:1174-85. 2008
    ..In addition, cleavable ICAT, which selectively tags cysteine-containing proteins, was also used, and the results complemented those obtained ..
  57. ncbi Analysis of microdissected cells by two-dimensional LC-MS approaches
    Chen Li
    Research Center for Proteome Analysis, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China
    Methods Mol Biol 428:193-208. 2008
    ..We combined the LCM with isotope-coded affinity tag (ICAT) technology and two-dimensional liquid chromatography to investigate the qualitative and quantitative proteomes of ..
  58. pmc Quantitative proteomics analysis of the effects of ionizing radiation in wild type and p53 K317R knock-in mouse thymocytes
    Lisa M Miller Jenkins
    Laboratory of Cell Biology, NCI, National Institutes of Health, Bethesda, Maryland 20892, USA
    Mol Cell Proteomics 7:716-27. 2008
    ..Using cleavable ICAT quantitative mass spectrometry, the effect of IR on protein levels in either the wild type or p53(K317R) thymocytes ..
  59. pmc Orai1 and Ca2+-independent phospholipase A2 are required for store-operated Icat-SOC current, Ca2+ entry, and proliferation of primary vascular smooth muscle cells
    Bo Yang
    Ion Channel and Calcium Signaling Unit, Boston Univ School of Medicine, Boston, MA 02118 2393, USA
    Am J Physiol Cell Physiol 302:C748-56. 2012
    ..This is the first demonstration that Orai1 and iPLA(2)β are equally important for cat-SOC, SOCE, and proliferation of primary aortic SMC...
  60. ncbi Quantitative proteomics analysis reveals that proteins differentially expressed in chronic pancreatitis are also frequently involved in pancreatic cancer
    Ru Chen
    Division of Gastroenterology, Department of Medicine, University of Washington, Seattle, Washington 98195, USA
    Mol Cell Proteomics 6:1331-42. 2007
    ..To better understand the limitations of potential protein biomarkers, we used ICAT technology and tandem mass spectrometry-based proteomics to systematically study protein expression in chronic ..
  61. ncbi Identification of redox sensitive thiols of protein disulfide isomerase using isotope coded affinity technology and mass spectrometry
    Anna Kozarova
    Department of Chemistry and Biochemistry, University of Windsor, Windsor, Ontario, Canada
    J Am Soc Mass Spectrom 18:260-9. 2007
    ..Here we describe a procedure utilizing isotope-coded affinity tag (ICAT) technology and mass spectrometry that quantitates relative changes in the dynamic thiol and disulfide states of ..
  62. ncbi Efficient synthesis of isotopically pure isotope-coded affinity tagging reagents
    Anupama Patel
    Department of Chemistry, 2036 Main Mall, University of British Columbia, Vancouver, B C V6T 1Z1 Canada, and PENCE Inc Protein Engineering Network of Centers of Excellence, 750 Heritage Medical Research Center, Edmonton, Alberta T6G 2S2 Canada
    Bioconjug Chem 15:224-30. 2004
    Synthesis of an isotopically pure d8-ICAT linker, N-[(5,5,6,6,8,8,9,9-2H)-13-biotinamido-4,7,10-trioxatridecanyl] tert-butyloxy carbamide (12), has been achieved in seven steps with an overall yield of 33%...
  63. ncbi Proteomic analysis of synaptosomes using isotope-coded affinity tags and mass spectrometry
    Sabine P Schrimpf
    Department of Biochemistry, University of Zurich, Zurich, Switzerland
    Proteomics 5:2531-41. 2005
    ..characterisation of synaptosomes isolated from mouse brain was performed employing the isotope-coded affinity tag (ICAT) method and tandem mass spectrometry (MS/MS)...
  64. ncbi Isotope-coded affinity tag (ICAT) approach to redox proteomics: identification and quantitation of oxidant-sensitive cysteine thiols in complex protein mixtures
    Mahadevan Sethuraman
    Vascular Biology Unit, Cardiovascular Proteomics Center, and Mass Spectrometry Resource, Boston University School of Medicine, Boston, MA 02118, USA
    J Proteome Res 3:1228-33. 2004
    ..cysteine thiols in a complex protein mixture using a thiol-specific, acid-cleavable isotope-coded affinity tag (ICAT) reagent (Applied Biosystems, USA)...
  65. ncbi The role of proteomics in toxicology: identification of biomarkers of toxicity by protein expression analysis
    Sandy Kennedy
    Oxford GlycoSciences UK Ltd, The Forum, 86 Milton Park, Abingdon, Oxfordshire OX14 4RY, UK
    Biomarkers 7:269-90. 2002
    ..The isotope-coded affinity tag (ICAT) method appears particularly promising...
  66. ncbi Affinity labeling of highly hydrophobic integral membrane proteins for proteome-wide analysis
    Michael B Goshe
    Biological Sciences Division, Pacific Northwest National Laboratory, PO Box 999, MSIN K8 98, Richland, Washington 99352, USA
    J Proteome Res 2:153-61. 2003
    ..08. The protocol described can be used with other stable isotope labeling reagents (e.g., ICAT) to enable comparative measurements to be made on differentially expressed hydrophobic membrane proteins from ..
  67. ncbi Proteomic analysis of the intestinal epithelial cell response to enteropathogenic Escherichia coli
    Philip R Hardwidge
    Biotechnology Laboratory, University of British Columbia, Wesbrook Building, 6174 University Boulevard, Vancouver, British Columbia V6T 1Z3, Canada
    J Biol Chem 279:20127-36. 2004
    ..Host proteins were isolated and subjected to quantitative profiling using isotope-coded affinity tagging (ICAT) combined with electrospray ionization tandem mass spectrometry...
  68. ncbi Infrequent somatic mutations of the ICAT gene in various human cancers with frequent 1p-LOH and/or abnormal nuclear accumulation of beta-catenin
    Mitsuho Imai
    Department of Molecular Pathology, Tohoku University School of Medicine, 2 1 Seiryo machi, Aoba ku, Sendai 980 8575, Japan
    Oncol Rep 12:1099-103. 2004
    ..b>ICAT, inhibitor of beta-catenin and TCF4/beta-catenin-interacting protein, was isolated and mapped to 1p36, a frequent ..
  69. ncbi Design and synthesis of visible isotope-coded affinity tags for the absolute quantification of specific proteins in complex mixtures
    Patricia Bottari
    Department of Chemistry, University of Washington, Seattle, Washington 98195, USA
    Bioconjug Chem 15:380-8. 2004
    ..We recently introduced isotope-coded affinity tags (ICAT reagents) for the relative quantification of proteins present in two or more biological samples...
  70. ncbi Controlling deuterium isotope effects in comparative proteomics
    Roujian Zhang
    Department of Chemistry, Purdue University, West Lafayette, Indiana 47907, USA
    Anal Chem 74:3662-9. 2002
    ..ester-2H9, [3-(2,5-dioxopyrrolidin-1-yloxycarbonyl)-propyl]-trimethylammonium chloride-2H9, and the commercial ICAT-2H8 reagent...
  71. ncbi The use of isotope-coded affinity tags (ICAT) to study organelle proteomes in Arabidopsis thaliana
    T P J Dunkley
    Department of Biochemistry, Cambridge Centre for Proteomics, University of Cambridge, Building O, Downing Site, Cambridge CB2 1QW, UK
    Biochem Soc Trans 32:520-3. 2004
    ..For this reason, quantitative proteomics methods are being developed to enable the assignment of proteins to a specific component of the endomembrane system without the need to obtain pure organelles...
  72. ncbi Femtomol sensitivity post-digest (18)O labeling for relative quantification of differential protein complex composition
    Marcus Bantscheff
    Cellzome AG, Meyerhofstrasse 1, 69117 Heidelberg, Germany
    Rapid Commun Mass Spectrom 18:869-76. 2004
    ..Chemical labeling of complex protein mixtures by, e.g., isotope-coded affinity tags (ICAT), can offer great simplification of protein mixtures but is restricted by the accessibility of the often few ..
  73. pmc Utility of cleavable isotope-coded affinity-tagged reagents for quantification of low-copy proteins induced by methylprednisolone using liquid chromatography/tandem mass spectrometry
    Jun Qu
    The Department of Pharmaceutical Sciences, University at Buffalo, State University of New York, Amherst, New York 14260 1200, USA
    Anal Chem 78:4543-52. 2006
    ..To our knowledge, this is the first report of an ICAT-based method that is capable of quantifying low-abundance proteins in highly complex samples, such as tissue ..
  74. pmc A novel GTP-dependent mechanism of ileal muscarinic metabotropic channel desensitization
    A V Zholos
    Department of Pharmacology and Clinical Pharmacology, St George s Hospital Medical School, London
    Br J Pharmacol 119:997-1005. 1996
    1. Cationic current (Icat) was evoked in single isolated smooth muscle cells either by activating muscarinic receptors with the stable muscarinic agonist, carbachol (CCh), or by dialysing cells with GTP-gamma S...
  75. ncbi Inhibition of PACAP activity by a receptor antagonist results in changes in cell cycle and apoptotic proteins in chick neuroblasts
    Nola M Erhardt
    Department of Biology, University of Victoria, Victoria, British Columbia, Canada
    J Mol Neurosci 27:107-23. 2005
    ..processes, we blocked the primary PACAP receptor and determined protein changes using isotope-coded affinity tag (ICAT) analysis...
  76. ncbi Screening for gene mutations in a 500 kb neuroblastoma tumor suppressor candidate region in chromosome 1p; mutation and stage-specific expression in UBE4B/UFD2
    Cecilia Krona
    Department of Clinical Genetics Institute for the Health of Women and Children, Goteborg University, Sahlgrenska University Hospital East, Goteborg, Sweden
    Oncogene 22:2343-51. 2003
    ..known to reside in or very close to this overlap consensus region, UBE4B/UFD2, KIF1B, DFFA, PGD, CORT, PEX14, and ICAT, for coding mutations in NBL tumor DNA...
  77. ncbi Accuracy of reconstructed images from cone-beam computed tomography scans
    Manish Lamichane
    Department of Developmental Biology, School of Dental Medicine, Harvard University, Boston, Mass, USA
    Am J Orthod Dentofacial Orthop 136:156.e1-6. 2009
    ..determine whether 2-dimensional (2D) images produced from cone-beam computed tomography (CBCT) images taken with an iCAT scanner (Imaging Sciences International, Hatfield, Pa) can substitute for traditional cephalograms...
  78. ncbi Proteomic characteristics of ex vivo-enriched adult human bone marrow mononuclear cells in continuous perfusion cultures
    Agasanur K Prahalad
    Anatomy and Cell Biology, Indiana University School of Medicine, Indianapolis, Indiana 46202, USA
    J Proteome Res 8:2079-89. 2009
    ..Isotope-coded affinity tags (ICAT) and MS/MS were used to identify and quantify relative changes in the proteome of BMMNC and their related BMMCP, ..
  79. doi WNT/beta-catenin pathway activation in Wilms tumors: a unifying mechanism with multiple entries?
    Marie Corbin
    INSERM, U574, Hopital Necker Enfants Malades, Paris, France
    Genes Chromosomes Cancer 48:816-27. 2009
    ..2), CTNNBIP1 (1p36.2), and CBY1 (22q13.1). These genes thus represent new potential important actors in WT tumorigenesis...
  80. ncbi Application of proteomics to the study of molecular mechanisms in neurotoxicology
    Richard M LoPachin
    Department of Anesthesiology, Montefiore Medical Center, Albert Einstein College of Medicine, Moses 7, 111 E, 210th St, Bronx, NY 10467, USA
    Neurotoxicology 24:761-75. 2003
    ..g. two-hybrid systems, antibody arrays, protein chips, isotope-coded affinity tags, ICAT) are available to determine protein levels, identify components of multiprotein complexes and to detect post-..
  81. pmc Transcriptional regulation of SM22alpha by Wnt3a: convergence with TGFbeta(1)/Smad signaling at a novel regulatory element
    Shawn L Shafer
    Washington University School of Medicine, Center for Cardiovascular Research, Internal Medicine BMD, Campus Box 8301, 660 South Euclid Ave, St Louis, MO 63110, USA
    J Mol Cell Cardiol 46:621-35. 2009
    ..Dominant negative TCF inhibited SM22alpha [-213 to -192] x 6 RSVLUC activation. Moreover, ICAT (inhibitor of beta-catenin and TCF) decreased while TCF7L2 and beta-catenin enhanced 0...
  82. doi CysTRAQ - A combination of iTRAQ and enrichment of cysteinyl peptides for uncovering and quantifying hidden proteomes
    Vojtech Tambor
    Institute of Molecular Pathology, Faculty of Military Health Sciences, University of Defence, Hradec Kralove, Czech Republic
    J Proteomics 75:857-67. 2012
    ..We herein show that CysTRAQ is a robust and straightforward method with potential application in quantitative proteomics experiments, i.e. as an alternative to the ICAT reagent approach.
  83. doi Influence of voxel size in the diagnostic ability of cone beam tomography to evaluate simulated external root resorption
    Gabriela Salatino Liedke
    Department of Surgery and Orthopedics, School of Dentistry, Federal University of Rio Grande do Sul UFRGS, Brazil
    J Endod 35:233-5. 2009
    ..For that purpose, 59 teeth were viewed through iCAT tomography (Imaging Sciences International, Inc, Hatfield, PA) following three protocols in which the variation was ..
  84. pmc A genome-wide screen for beta-catenin binding sites identifies a downstream enhancer element that controls c-Myc gene expression
    Gregory S Yochum
    Vollum Institute and Department of Medicine, Oregon Health and Science University, 3181 SW Sam Jackson Park Rd, Portland, OR 97239, USA
    Mol Cell Biol 28:7368-79. 2008
    ..Our findings indicate that a downstream enhancer element provides the principal regulation of c-Myc expression...
  85. ncbi Cyclin-dependent kinase 2 regulates the interaction of Axin with beta-catenin
    Sung Il Kim
    Department of Life Science, Kwangju Institute of Science and Technology, Kwangju 500 712, Republic of Korea
    Biochem Biophys Res Commun 317:478-83. 2004
    ..This suggests that cyclin A/CDK2 is a negative regulator of beta-catenin-mediated signal transduction, which exerts its effects through phosphorylation of Axin...
  86. pmc Quantitative proteomics identifies oxidant-induced, AtMPK6-dependent changes in Arabidopsis thaliana protein profiles
    Godfrey P Miles
    Michael Smith Laboratories, University of British Columbia, Vancouver, BC, CA, USA
    Plant Signal Behav 4:497-505. 2009
    ..whose accumulation is altered by ozone in an AtMPK6-dependent manner we employed isotope-coded affinity tagging (ICAT) technology to investigate the impact of AtMPK6-suppression on the protein profiles in Arabidopsis both before (air ..
  87. doi Trans-proteomic pipeline: a pipeline for proteomic analysis
    Patrick G A Pedrioli
    Institute of Biochemistry, Swiss Federal Institute of Technology Zurich ETHZ, Zurich, Switzerland
    Methods Mol Biol 604:213-38. 2010
    ..and supports most commonly used proteomics workflows, including quantitative applications such as ICAT, iTRAQ, and SILAC...
  88. ncbi ICAT inhibits beta-catenin binding to Tcf/Lef-family transcription factors and the general coactivator p300 using independent structural modules
    Danette L Daniels
    Department of Structural Biology, Stanford University School of Medicine, Stanford, CA 94305, USA
    Mol Cell 10:573-84. 2002
    ..The crystal structure of ICAT, an inhibitor of beta-catenin-mediated transcription, bound to the armadillo repeat domain of beta-catenin, has ..
  89. ncbi Molecular cloning and characterization of LZIC, a novel gene encoding ICAT homologous protein with leucine zipper domain
    M Katoh
    Genetics and Cell Biology Section, Genetics Division, National Cancer Center Research Institute, Chuo Ku, Tokyo 104 0045, Japan
    Int J Mol Med 8:611-5. 2001
    b>ICAT inhibits the interaction between beta-catenin and TCF transcription factors...
  90. ncbi Toward a high-throughput approach to quantitative proteomic analysis: expression-dependent protein identification by mass spectrometry
    T J Griffin
    Department of Molecular Biotechnology, University of Washington, Seattle, USA
    J Am Soc Mass Spectrom 12:1238-46. 2001
    The isotope-coded affinity tag (ICAT) technology enables the concurrent identification and comparative quantitative analysis of proteins present in biological samples such as cell and tissue extracts and biological fluids by mass ..
  91. ncbi Molecular genetic analysis of malignant melanomas for aberrations of the WNT signaling pathway genes CTNNB1, APC, ICAT and BTRC
    Julia Reifenberger
    Department of Dermatology, Heinrich Heine University, Dusseldorf, Germany
    Int J Cancer 100:549-56. 2002
    ..2), BTRC (beta-transducin repeat-containing protein gene, 10q24.3) and ICAT (inhibitor of beta-catenin and Tcf-4, 1p36.2)...
  92. pmc Inhibition of β-catenin signaling in chondrocytes induces delayed fracture healing in mice
    Yang Huang
    The Key Laboratory of Stem Cell Biology, Institute of Health Sciences, Shanghai Institutes for Biological Sciences SIBS, Chinese Academy of Sciences CAS and Shanghai Jiao Tong University School of Medicine SJTUSM, Shanghai 200025, China
    J Orthop Res 30:304-10. 2012
    ..role and importance of this pathway in chondrocytes, we studied bone repair of closed tibias fractures in Col2a1-ICAT transgenic mice, in which the Wnt/β-catenin signaling pathway is specially inhibited in chondrocytes...
  93. doi MicroRNA-23 restricts cardiac valve formation by inhibiting Has2 and extracellular hyaluronic acid production
    Anne Karine Lagendijk
    Hubrecht Institute for Developmental Biology and Stem Cell Research, CT Utrecht, The Netherlands
    Circ Res 109:649-57. 2011
    ..Although roles for microRNAs in the myocardium during embryo development and cardiac disease have been demonstrated, very little is know about their role in the endocardium or during cardiac valve formation...
  94. doi Additive renoprotective effects of B2-kinin receptor blocker and PPAR-γ agonist in uninephrectomized db/db mice
    Sydney C W Tang
    Division of Nephrology, Department of Medicine, Queen Mary Hospital, The University of Hong Kong, Hong Kong
    Lab Invest 91:1351-62. 2011
    We recently showed that the bradykinin B2 receptor (B2R) blocker icatibant (Icat) and the peroxisome proliferator-activated receptor-γ agonist rosiglitazone (Ros) exerted anti-inflammatory effects in renal tubular cells exposed to a ..
  95. pmc Supporting the Collaborative Authoring of ICD-11 with WebProtégé
    Tania Tudorache
    Stanford Center for Biomedical Research, Stanford University, Stanford, CA
    AMIA Annu Symp Proc 2010:802-6. 2010
    ..We present the ICD Collaborative Authoring Tool (iCAT), a customization of the WebProtégé editor that supports the community based authoring of ICD-11 on the Web and ..
  96. doi EBV-encoded LMP1 increases nuclear β-catenin accumulation and its transcriptional activity in nasopharyngeal carcinoma
    Shuyuan You
    Department of Pathology, The First Affiliated Hospital and Zhongshan School of Medicine, Sun Yat Sen University, 58, Zhongshan Road II, Guangzhou, 510080, China
    Tumour Biol 32:623-30. 2011
    ..Moreover, LMP1 expression was closely related to abnormal β-catenin expression in NPC tissues by immunohistochemistry. LMP1 may be involved in nasopharyngeal carcinogenesis via β-catenin signaling pathway...
  97. doi Quantitative proteomic analysis of S-nitrosated proteins in diabetic mouse liver with ICAT switch method
    Xu Zhang
    National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, 100101, China
    Protein Cell 1:675-87. 2010
    In this study we developed a quantitative proteomic method named ICAT switch by introducing isotope-coded affinity tag (ICAT) reagents into the biotin-switch method, and used it to investigate S-nitrosation in the liver of normal control ..
  98. doi Altered β-catenin expression related to cancer progression on actinic cheilitis and squamous cell carcinoma of the lip
    Juliana L Schussel
    Department of Oral Pathology, School of Dentistry, University of Sao Paulo USP, Brazil
    Ann Diagn Pathol 15:1-5. 2011
    ..Cytoplasmatic and nuclear staining of β-catenin on studied cases point to pathway alterations. Results demonstrated that β-catenin expression is altered on epithelial dysplasia, and it is related to degree of alterations...
  99. ncbi Involvement of the Wnt-β-catenin pathway in invasion and migration of oral squamous carcinoma cells
    Soichi Iwai
    Department of Oral and Maxillofacial Surgery II, Osaka University Graduated School of Dentistry, Suita, Osaka 565 0871, Japan
    Int J Oncol 37:1095-103. 2010
    ..This would enhance the invasion and migration of oral SCC cells...
  100. doi Direct comparison of stable isotope labeling by amino acids in cell culture and spectral counting for quantitative proteomics
    Timothy S Collier
    W M Keck FT ICR Mass Spectrometry Laboratory, Department of Chemistry, North Carolina State University, Raleigh, North Carolina 27695, USA
    Anal Chem 82:8696-702. 2010
    ..metabolic labeling, stable isotope labeling by amino acids in cell culture (SILAC)), chemical derivatization (ICAT, iTRAQ, TMT), or enzymatically catalyzed incorporation (¹⁸O labeling)...
  101. pmc Ph+/VE-cadherin+ identifies a stem cell like population of acute lymphoblastic leukemia sustained by bone marrow niche cells
    Lin Wang
    Department of Pediatrics, and Mary Babb Randolph Cancer Center, West Virginia University School of Medicine, Morgantown, WV 26506, USA
    Blood 110:3334-44. 2007
    ..These data suggest that strategies targeting signals in the marrow microenvironment that amplify the Bcr-abl/VE-cadherin/beta-catenin axis may have utility in sensitizing drug-resistant leukemic stem cells...

Research Grants81

  1. Directed Proteomics of Alzheimer's Related Proteins
    ELIZABETH KOMIVES; Fiscal Year: 2007
    ..diseased brains will be monitored and interacting partners will be quantified using isotope labeling strategies (ICAT)...
  2. Acquisition of a LC-MS/MS Proteomics System
    David Han; Fiscal Year: 2004
    ..Dr. David Han will utilize 3D-ICAT technology to identify and quantify apoptotic signaling proteins and engulfment ligands that are presented on ..
  3. Functional Proteomics Study of Ephrin Signaling
    Thomas Neubert; Fiscal Year: 2003
    ..We propose to develop and use a new functional proteomics method based on isotope-coded affinity tag (ICAT) chemistry and liquid chromatography/mass spectrometry to identify proteins that participate in signal transduction ..
  4. 2D-HPLC-Tandem Mass Spectrometer for Proteomics
    Richard van Breemen; Fiscal Year: 2005
    ..used by many NIH-funded investigators for proteomic profiling using techniques such as isotope-coded affinity tags (ICAT)...
  5. FUNCTIONAL PROTEOMICS OF LEUKEMIC MARROW STROMAL CELLS
    Beerelli Seshi; Fiscal Year: 2007
    ..identify about 200 differentially expressed MPC proteins by an independent method, isotope-coded affinity tag (ICAT) labeling in conjunction with LC/MS/MS...
  6. ProteomeX LC/MS System
    Michael Powell; Fiscal Year: 2004
    ..package for typical proteomics applications such as protein ID, multidimensional LC/MS, and protein profiling using ICAT reagents. The system builds on the very successful and popular LCQ Deca XP...
  7. Proteomics processing using networked instrument router*
    SANJAYA JOSHI; Fiscal Year: 2005
    ..The First Phase of study will use an LC-MS (Liquid Chromatography - Mass Spectroscopy) system that uses the ICAT (Isotope Coded Affinity Tags) technology developed at the lab of Dr...
  8. Proteome-Wide Characterization of Phosphoproteins
    Richard Morrison; Fiscal Year: 2004
    ..The later phase of this project will develop methods that concomitantly combine PhIAT and ICAT labeling to identify proteins in glutamate or camptothecin treated p53+/+ cortical neurons that undergo changes in ..
  9. ApoE isoform-specific protein interactions in brain aging and neurodegeneration
    KATHLEEN MONTINE; Fiscal Year: 2007
    ..developed approach of high-throughput quantitative proteomic analysis combining isotope-coded affinity tagging (ICAT) with peptide analysis by LC/MS-MS allows us to identify and compare the relative abundance of apoE-associated ..
  10. Mitochondrial Proteome Changes in Alzheimer's Disease
    Bert Lynn; Fiscal Year: 2007
    ..liquid chromatography coupled with tandem mass spectrometry (2D-LC/MS/MS) and the isotope coded affinity tag (ICAT) method to identify and quantify protein levels in mitochondria isolated from early AD, late stage AD and age-..
  11. Novel markers for neonatal sepsis
    Ashok Reddy; Fiscal Year: 2005
    ..SELDI-TOF) mass spectrometry, (b) two-dimensional gel electrophoresis, and (c) isotope-coded affinity tagging (ICAT) to detect differentially expressed proteins...
  12. UV-induced Immunosuppression to chemical toxicants
    Kevin Cooper; Fiscal Year: 2006
    ..broad identification of changes in the abundance of membrane-bound proteins using isotope coded affinity tagging (ICAT) and phosphoproteins using phosphoprotein isotope-coded affinity tags (PhlAT)...
  13. Proteomic Analysis of Renal Response to Acidosis
    Norman Curthoys; Fiscal Year: 2007
    ..Further analysis using the ICAT labeling reagent identified many of the same proteins with similar fold differences...
  14. LIQUID CHROMATOGRAPHY OF BIOLOGICAL SUBSTANCES
    Csaba Horvath; Fiscal Year: 2003
    ..We shall start with improving the separation steps of a promising analytical method referred to as ICAT technology. Further we plan to enhance the speed of analysis of this approach by using elevated temperature...
  15. LIQUID CHROMATOGRAPHY OF BIOLOGICAL SUBSTANCES
    LISA PFEFFERLE; Fiscal Year: 2005
    ..We shall start with improving the separation steps of a promising analytical method referred to as ICAT technology. Further we plan to enhance the speed of analysis of this approach by using elevated temperature...
  16. LIQUID CHROMATOGRAPHY OF BIOLOGICAL SUBSTANCES
    James Wilkins; Fiscal Year: 2004
    ..We shall start with improving the separation steps of a promising analytical method referred to as ICAT technology. Further we plan to enhance the speed of analysis of this approach by using elevated temperature...
  17. Molecular Definition of Mycobacterium paratuberculosis
    JULIA INAMINE; Fiscal Year: 2005
    ..This goal will be accomplished by four specific aims: 1) use standard proteomic methods and develop a new ICAT- based method to identify M...
  18. A Novel Implant-based Approach for Treating Retrognathic Hyperdivergent Patients
    Peter Buschang; Fiscal Year: 2007
    ..Tipping will be measured directly from dental models and 3D ICAT images...
  19. Potassium Channels as Macromolecular Complexes
    Thomas A Neubert; Fiscal Year: 2010
    ..Protein interactions will also be investigated using advanced proteomic approaches (ICAT &ITRAQ), which has the potential to uncover additional novel KATP channel interacting proteins...
  20. Potassium Channels as Macromolecular Complexes
    WILLIAM COETZEE; Fiscal Year: 2009
    ..Protein interactions will also be investigated using advanced proteomic approaches (ICAT & ITRAQ), which has the potential to uncover additional novel KATP channel interacting proteins...
  21. The use of genetic models to define the role of beta-catenin in post-natal growth
    REGIS J O apos KEEFE; Fiscal Year: 2010
    ..b>ICAT is an intracellular protein that competitively binds ?-catenin and inhibits signaling...
  22. The use of genetic models to define the role of beta-catenin in post-natal growth
    REGIS O KEEFE; Fiscal Year: 2009
    ..b>ICAT is an intracellular protein that competitively binds ?-catenin and inhibits signaling...
  23. The use of genetic models to define the role of beta-catenin in post-natal growth
    REGIS O KEEFE; Fiscal Year: 2007
    ..b>ICAT is an intracellular protein that competitively binds ?-catenin and inhibits signaling...
  24. Proteomics Approaches to Interstitial Cystitis.
    Brian Liu; Fiscal Year: 2006
    ..levels of low- abundance urinary proteins in clinically annotated IC specimens with isotope-coded affinity tag (ICAT) and mass spectrometry; 4) mine proteomics data and to create predictive bioinformatics models (i.e...
  25. MICROBICIDAL SALIVARY HISTIDINE-RICH PROTEINS
    Frank G Oppenheim; Fiscal Year: 2011
    ..These include isotope coded affinity tag (ICAT), multiplexed isobaric isotope reagent (iTRAQ) and metabolic labeling...
  26. MICROBICIDAL SALIVARY HISTIDINE-RICH PROTEINS
    Frank G Oppenheim; Fiscal Year: 2010
    ..These include isotope coded affinity tag (ICAT), multiplexed isobaric isotope reagent (iTRAQ) and metabolic labeling...
  27. Calcium signaling in cerebral arteries
    Jonathan H Jaggar; Fiscal Year: 2010
    ..suggest that in addition to mobilizing SR Ca2+, IP3 activates a plasma membrane non-selective cation current (ICat) independently of SR release, and this leads to vasoconstriction...
  28. Mitochondrial Response to Oxidative Stress
    DANIEL BOGENHAGEN; Fiscal Year: 2006
    ..Both 2-D gel methods and quantitative isotope-coded affinity tag (ICAT) methods will be used to compare the abundance of mitochondrial proteins in control cells and cells exposed to ..
  29. Regulation of beta-Catenin Signaling by Tyrosine Phosphorylation
    Tong Chuan He; Fiscal Year: 2007
    ..second aim is to determine whether EGF regulates the interactions of b-catenin with its C-terminal binding partners ICAT and p300, by using immunoprecipitation and FRET protein interaction analysis...
  30. Regulation of beta-Catenin Signaling by Tyrosine Phosphorylation
    Tong Chuan He; Fiscal Year: 2010
    ..second aim is to determine whether EGF regulates the interactions of P-catenin with its C-terminal binding partners ICAT and p300, by using immunoprecipitation and FRET protein interaction analysis...
  31. A PROTEOMIC APPROACH TO THE INDENTIFICATION OF PROTEINS*
    Philip Mirkes; Fiscal Year: 2003
    ..by undertaking a quantitative analysis of complex embryo protein mixtures using isotope-coded affinity tags (ICAT)...
  32. Mechanisms of prostate cancer prevention by lycopene
    Richard van Breemen; Fiscal Year: 2007
    ..lycopene and then investigate changes in protein expression both globally and on the sub-cellular level using the ICAT quantitative proteomics methodology...