HUWE1

Summary

Gene Symbol: HUWE1
Description: HECT, UBA and WWE domain containing 1, E3 ubiquitin protein ligase
Alias: ARF-BP1, HECTH9, HSPC272, Ib772, LASU1, MULE, URE-B1, UREB1, E3 ubiquitin-protein ligase HUWE1, ARF-binding protein 1, BJ-HCC-24 tumor antigen, HECT domain protein LASU1, HECT-type E3 ubiquitin transferase HUWE1, Mcl-1 ubiquitin ligase E3, URE-binding protein 1, homologous to E6AP carboxyl terminus homologous protein 9, large structure of UREB1, upstream regulatory element-binding protein 1
Species: human
Products:     HUWE1

Top Publications

  1. Zhong Q, Gao W, Du F, Wang X. Mule/ARF-BP1, a BH3-only E3 ubiquitin ligase, catalyzes the polyubiquitination of Mcl-1 and regulates apoptosis. Cell. 2005;121:1085-95 pubmed
    ..fractionation of cell extracts allowed us to identify a 482 kDa HECT-domain-containing ubiquitin ligase named Mule (Mcl-1 ubiquitin ligase E3) that is both required and sufficient for the polyubiquitination of Mcl-1...
  2. Pervin S, Tran A, Tran L, Urman R, Braga M, Chaudhuri G, et al. Reduced association of anti-apoptotic protein Mcl-1 with E3 ligase Mule increases the stability of Mcl-1 in breast cancer cells. Br J Cancer. 2011;105:428-37 pubmed publisher
    ..Although Mule, an E3 ligase that targets Mcl-1 for degradation was expressed in mammary epithelial and breast cancer cell lines, ..
  3. Liu Z, Oughtred R, Wing S. Characterization of E3Histone, a novel testis ubiquitin protein ligase which ubiquitinates histones. Mol Cell Biol. 2005;25:2819-31 pubmed
    ..the final material revealed the presence of a 482-kDa HECT domain-containing protein, which was previously named LASU1. Anti-LASU1 antibodies immunodepleted E3Histone activity...
  4. Adhikary S, Marinoni F, Hock A, Hulleman E, Popov N, Beier R, et al. The ubiquitin ligase HectH9 regulates transcriptional activation by Myc and is essential for tumor cell proliferation. Cell. 2005;123:409-21 pubmed
    ..Here we show that the E3 ubiquitin ligase HectH9 ubiquitinates Myc in vivo and in vitro, forming a lysine 63-linked polyubiquitin chain...
  5. Herold S, Hock A, Herkert B, Berns K, Mullenders J, Beijersbergen R, et al. Miz1 and HectH9 regulate the stability of the checkpoint protein, TopBP1. EMBO J. 2008;27:2851-61 pubmed publisher
    ..TopBP1 that is not bound to chromatin is degraded by the HectH9 (Mule, ARF-BP1 and HUWE1) ubiquitin ligase...
  6. Schwickart M, Huang X, Lill J, Liu J, Ferrando R, French D, et al. Deubiquitinase USP9X stabilizes MCL1 and promotes tumour cell survival. Nature. 2010;463:103-7 pubmed publisher
    ..These results identify USP9X as a prognostic and therapeutic target, and they show that deubiquitinases may stabilize labile oncoproteins in human malignancies. ..
  7. Noy T, Suad O, Taglicht D, Ciechanover A. HUWE1 ubiquitinates MyoD and targets it for proteasomal degradation. Biochem Biophys Res Commun. 2012;418:408-13 pubmed publisher
    ..Here we describe the involvement of HUWE1 in the ubiquitination and proteasomal degradation of MyoD...
  8. Parsons J, Tait P, Finch D, Dianova I, Edelmann M, Khoronenkova S, et al. Ubiquitin ligase ARF-BP1/Mule modulates base excision repair. EMBO J. 2009;28:3207-15 pubmed publisher
    ..By fractionating human cell extract, we purified the E3 ubiquitin ligase Mule (ARF-BP1/HectH9) as an enzyme that can ubiquitylate DNA polymerase beta (Pol beta), the major BER DNA polymerase...
  9. Pandya R, Partridge J, Love K, Schwartz T, Ploegh H. A structural element within the HUWE1 HECT domain modulates self-ubiquitination and substrate ubiquitination activities. J Biol Chem. 2010;285:5664-73 pubmed publisher
    ..Here we present a functional analysis of the HECT domain of the E3 ligase HUWE1 based on crystal structures and show that a single N-terminal helix significantly stabilizes the HECT domain...

More Information

Publications106 found, 100 shown here

  1. Zhang J, Kan S, Huang B, Hao Z, Mak T, Zhong Q. Mule determines the apoptotic response to HDAC inhibitors by targeted ubiquitination and destruction of HDAC2. Genes Dev. 2011;25:2610-8 pubmed publisher
    ..apoptosis are severely compromised in mouse embryonic fibroblasts lacking a HECT domain ubiquitin ligase, Mule (Mcl-1 ubiquitin ligase E3). Mule specifically targets HDAC2 for ubiquitination and degradation...
  2. Chen D, Kon N, Li M, Zhang W, Qin J, Gu W. ARF-BP1/Mule is a critical mediator of the ARF tumor suppressor. Cell. 2005;121:1071-83 pubmed
    ..As such, ARF-BP1 may serve as a potential target for therapeutic intervention in tumors regardless of p53 status. ..
  3. Hall J, Kow E, Nevis K, Lu C, Luce K, Zhong Q, et al. Cdc6 stability is regulated by the Huwe1 ubiquitin ligase after DNA damage. Mol Biol Cell. 2007;18:3340-50 pubmed
    ..Cdc6 directly binds a HECT-family ubiquitin E3 ligase, Huwe1 (also known as Mule, UreB1, ARF-BP1, Lasu1, and HectH9), and Huwe1 polyubiquitinates Cdc6 in vitro...
  4. Yin L, Joshi S, Wu N, Tong X, Lazar M. E3 ligases Arf-bp1 and Pam mediate lithium-stimulated degradation of the circadian heme receptor Rev-erb alpha. Proc Natl Acad Sci U S A. 2010;107:11614-9 pubmed publisher
    ..Thus, Arf-bp1 and Pam are novel regulators of circadian gene expression that target Rev-erb alpha for degradation. ..
  5. Warr M, Acoca S, Liu Z, Germain M, Watson M, Blanchette M, et al. BH3-ligand regulates access of MCL-1 to its E3 ligase. FEBS Lett. 2005;579:5603-8 pubmed
    ..using position weight matrices (PWM) and identified a large (480kDa), novel BH3-only protein, originally called LASU1 (now also known as Ureb-1, E3(histone), ARF-BP1, and Mule)...
  6. Froyen G, Corbett M, Vandewalle J, Jarvela I, Lawrence O, Meldrum C, et al. Submicroscopic duplications of the hydroxysteroid dehydrogenase HSD17B10 and the E3 ubiquitin ligase HUWE1 are associated with mental retardation. Am J Hum Genet. 2008;82:432-43 pubmed publisher
    ..The minimal, commonly duplicated region contains three genes: RIBC1, HSD17B10, and HUWE1. RIBC1 could be excluded on the basis of its absence of expression in the brain and because it escapes X ..
  7. Zhao X, Heng J, Guardavaccaro D, Jiang R, Pagano M, Guillemot F, et al. The HECT-domain ubiquitin ligase Huwe1 controls neural differentiation and proliferation by destabilizing the N-Myc oncoprotein. Nat Cell Biol. 2008;10:643-53 pubmed publisher
    ..Here we report that the HECT-domain ubiquitin ligase Huwe1 ubiquitinates the N-Myc oncoprotein through Lys 48-mediated linkages and targets it for destruction by the ..
  8. Stankiewicz A, Livingstone A, Mohseni N, Mosser D. Regulation of heat-induced apoptosis by Mcl-1 degradation and its inhibition by Hsp70. Cell Death Differ. 2009;16:638-47 pubmed publisher
    ..Heat shock caused the dissociation of Noxa from Mcl-1, which allowed binding of the BH3-containing ubiquitin ligase Mule followed by Mcl-1 ubiquitination and degradation...
  9. Yang Y, Do H, Tian X, Zhang C, Liu X, Dada L, et al. E3 ubiquitin ligase Mule ubiquitinates Miz1 and is required for TNFalpha-induced JNK activation. Proc Natl Acad Sci U S A. 2010;107:13444-9 pubmed publisher
    ..However, the underling regulatory mechanism is not known. Here, we report that HECT-domain-containing Mule is the E3 ligase that catalyzes TNFalpha-induced Miz1 polyubiquitination...
  10. Hao Z, Duncan G, Su Y, Li W, Silvester J, Hong C, et al. The E3 ubiquitin ligase Mule acts through the ATM-p53 axis to maintain B lymphocyte homeostasis. J Exp Med. 2012;209:173-86 pubmed publisher
    Cellular homeostasis is controlled by pathways that balance cell death with survival. Mcl-1 ubiquitin ligase E3 (Mule) is an E3 ubiquitin ligase that targets the proapoptotic molecule p53 for polyubiquitination and degradation...
  11. Mu J, Wang Y, Luo H, Leng M, Zhang J, Yang T, et al. A proteomic analysis of ataxia telangiectasia-mutated (ATM)/ATM-Rad3-related (ATR) substrates identifies the ubiquitin-proteasome system as a regulator for DNA damage checkpoints. J Biol Chem. 2007;282:17330-4 pubmed
  12. Froyen G, Belet S, Martinez F, Santos Rebouças C, Declercq M, Verbeeck J, et al. Copy-number gains of HUWE1 due to replication- and recombination-based rearrangements. Am J Hum Genet. 2012;91:252-64 pubmed publisher
    ..22 in individuals with nonsyndromic intellectual disability (ID) harboring HSD17B10, HUWE1, and the microRNAs miR-98 and let-7f-2 in the smallest region of overlap...
  13. Kodama T, Newberg J, Kodama M, Rangel R, Yoshihara K, Tien J, et al. Transposon mutagenesis identifies genes and cellular processes driving epithelial-mesenchymal transition in hepatocellular carcinoma. Proc Natl Acad Sci U S A. 2016;113:E3384-93 pubmed publisher
    ..receptor tyrosine kinase), GRB2-associated binding protein 1 (GAB1), HECT, UBA, and WWE domain containing 1 (HUWE1), lysine-specific demethylase 6A (KDM6A), and protein-tyrosine phosphatase, nonreceptor-type 12 (PTPN12), showed ..
  14. Gutiérrez Expósito D, García Bocanegra I, Howe D, Arenas Montés A, Yeargan M, Ness S, et al. A serosurvey of selected cystogenic coccidia in Spanish equids: first detection of anti-Besnoitia spp. specific antibodies in Europe. BMC Vet Res. 2017;13:128 pubmed publisher
    ..The principal risk factors associated with a higher seroprevalence of Besnoitia spp. were the host species (mule or donkey), the absence of shelter and the absence of a rodent control programme...
  15. Xiao S, Chen S, Cheng X, Lin F, Wang S, Zhu X, et al. The newly emerging duck-origin goose parvovirus in China exhibits a wide range of pathogenicity to main domesticated waterfowl. Vet Microbiol. 2017;203:252-256 pubmed publisher
    ..Our previous studies have found that SBDSV was highly pathogenic to Cherry Valley ducklings and mule ducklings. However, little is known about its pathogenicity to other waterfowls...
  16. Bai Y, Wang G, Fu W, Lu Y, Wei W, Chen W, et al. Circulating essential metals and lung cancer: Risk assessment and potential molecular effects. Environ Int. 2019;127:685-693 pubmed publisher
    ..could regulate the expressions of 8 cancer-related genes, including SOD1, APE, TP53BP1, WDR33, LAPTM4B, TRIT1, HUWE1, and ZNF813, which were over-expressed in lung tumor tissues...
  17. Verocai G, NELSON K, Callahan R, Wekesa J, Hassan H, Hoberg E. A cryptic species of Onchocerca (Nematoda: Onchocercidae) in blackflies (Simulium spp.) from southern California, USA. Parasit Vectors. 2018;11:547 pubmed publisher
    ..that this cryptic species of Onchocerca may infect subspecies of white-tailed deer (Odocoileus virginianus), and mule and black-tailed deer (Odocoileus hemionus) at temporal latitudes. The blackflies Simulium tescorum and S...
  18. Henderson J, Nisperos S, Weeks J, Ghulam M, Marín I, Zayas R. Identification of HECT E3 ubiquitin ligase family genes involved in stem cell regulation and regeneration in planarians. Dev Biol. 2015;404:21-34 pubmed publisher
    ..all HECT E3 ligases, we inhibited their expression using RNA interference (RNAi) and determined that orthologs of huwe1, wwp1, and trip12 had roles in tissue regeneration...
  19. Li Z, Peng Z, Gu S, Zheng J, Feng D, Qin Q, et al. Global Analysis of miRNA-mRNA Interaction Network in Breast Cancer with Brain Metastasis. Anticancer Res. 2017;37:4455-4468 pubmed
    ..mRNAs for two genes [HECT, UBA and WWE domain containing 1 (HUWE1) and BCL2] were found to have the highest associations with miRNAs, which were down-regulated in brain metastasis ..
  20. TAMGUNEY G, Richt J, Hamir A, Greenlee J, Miller M, Wolfe L, et al. Salivary prions in sheep and deer. Prion. 2012;6:52-61 pubmed publisher
    ..5 to 1.7 log ID₅₀ U/ml. Similarly, inoculation of saliva samples from two mule deer with CWD transmitted prions to Tg(ElkPrP) mice with titers of -1.1 to -0.4 log ID₅₀ U/ml...
  21. Meyerovich K, Violato N, Fukaya M, Dirix V, Pachera N, Marselli L, et al. MCL-1 Is a Key Antiapoptotic Protein in Human and Rodent Pancreatic ?-Cells. Diabetes. 2017;66:2446-2458 pubmed publisher
    ..We found that the kinase GSK3?, the E3 ligases MULE and ?TrCP, and the deubiquitinase USP9x regulate cytokine-mediated MCL-1 protein turnover in rodent ?-cells...
  22. Pal D, Basu A. Protein kinase C-eta regulates Mcl-1 level via ERK1. Cell Signal. 2017;40:166-171 pubmed publisher
    ..Moreover, knockdown of Mule (Mcl-1 ubiquitin ligase) prevented Mcl-1 downregulation caused by PKCη deficiency...
  23. Vullo C, Carluccio A, Robbe D, Meligrana M, Petrucci L, Catone G. Guaiphenesin-ketamine-xylazine infusion to maintain anesthesia in mules undergoing field castration. Acta Vet Scand. 2017;59:67 pubmed publisher
    ..Time of anesthesia, time of surgery and time of recovery were recorded. Only one mule required an additional dose of xylazine to achieve a satisfactory depth of sedation...
  24. Boonstra R, Boutin S, Jung T, Krebs C, Taylor S. Impact of rewilding, species introductions and climate change on the structure and function of the Yukon boreal forest ecosystem. Integr Zool. 2018;13:123-138 pubmed publisher
    ..The natural range expansion of mountain lions (Puma concolor), mule deer (Odocoileus hemionus) and American marten (Martes americana) should have few ecosystem effects...
  25. Yatsenko S, Mittal P, Wood Trageser M, Jones M, Surti U, Edwards R, et al. Highly heterogeneous genomic landscape of uterine leiomyomas by whole exome sequencing and genome-wide arrays. Fertil Steril. 2017;107:457-466.e9 pubmed publisher
    ..genes that were implicated in leiomyomagenesis such as COL4A6, DCN, and AHR, as well as novel genes: NRG1, ADAM18, HUWE1, FBXW4, FBXL13, and CAPRIN1...
  26. Manne S, Kondru N, Nichols T, Lehmkuhl A, Thomsen B, Main R, et al. Ante-mortem detection of chronic wasting disease in recto-anal mucosa-associated lymphoid tissues from elk (Cervus elaphus nelsoni) using real-time quaking-induced conversion (RT-QuIC) assay: A blinded collaborative study. Prion. 2017;:1-16 pubmed publisher
    ..Chronic wasting disease (CWD) is a TSE affecting both wild and captive populations of cervids, including mule deer, white-tailed deer, elk, moose, muntjac, and most recently, wild reindeer...
  27. Liu L, Yan X, Kong X, Zhao Y, Gong Z, Jin J, et al. Transcriptional Gene Silencing Maintained by OTS1 SUMO Protease Requires a DNA-Dependent Polymerase V-Dependent Pathway. Plant Physiol. 2017;173:655-667 pubmed publisher
    ..OTS1 gene is required for maintaining the silence of the reporter 35S-LUC and an endogenous mutator-like element MULE-F19G14 at the transcriptional level, which requires DNA-dependent RNA polymerase (Pol) V and DDR complex, but not ..
  28. Wang P, Wittmeyer K, Lee T, Meyers B, Chopra S. Overlapping RdDM and non-RdDM mechanisms work together to maintain somatic repression of a paramutagenic epiallele of maize pericarp color1. PLoS ONE. 2017;12:e0187157 pubmed publisher
    ..In addition, a MULE sequence within the proximal promoter of P1-wr shows depletion of 24nt siRNAs in mop1-1 plants...
  29. Benedek G, Meza Romero R, Jordan K, Keenlyside L, Offner H, Vandenbark A. HLA-DRα1-mMOG-35-55 treatment of experimental autoimmune encephalomyelitis reduces CNS inflammation, enhances M2 macrophage frequency, and promotes neuroprotection. J Neuroinflammation. 2015;12:123 pubmed publisher
    ..CD74, NLRP3, and IL-1β and increased expression of genes involved in myelin repair (MBP) and neuroregeneration (HUWE1)...
  30. Heidelberger J, Voigt A, Borisova M, Petrosino G, Ruf S, Wagner S, et al. Proteomic profiling of VCP substrates links VCP to K6-linked ubiquitylation and c-Myc function. EMBO Rep. 2018;19: pubmed publisher
    ..inhibition globally upregulates K6-linked ubiquitylation that is dependent on the HECT-type ubiquitin E3 ligase HUWE1. We report ~450 putative VCP substrates, many of which function in nuclear processes, including gene expression, ..
  31. Paderova J, Drabova J, Holubova A, Vlckova M, Havlovicova M, Gregorova A, et al. Under the mask of Kabuki syndrome: Elucidation of genetic-and phenotypic heterogeneity in patients with Kabuki-like phenotype. Eur J Med Genet. 2018;61:315-321 pubmed publisher
    ..while targeted exome sequencing revealed pathogenic variants in genes associated with intellectual disability (HUWE1, GRIN1), including a gene coding for mandibulofacial dysostosis with microcephaly (EFTUD2)...
  32. Vriend J, Ghavami S, Marzban H. The role of the ubiquitin proteasome system in cerebellar development and medulloblastoma. Mol Brain. 2015;8:64 pubmed publisher
    ..Data showing a role for the ubiquitin ligases β-TrCP, FBW7, Huwe1, and SKP2 in MBs suggest the possibility of a classification of MBs based on the expression (over expression or ..
  33. Vandewalle J, Langen M, Zschätzsch M, Zschaetzsch M, Nijhof B, Kramer J, et al. Ubiquitin ligase HUWE1 regulates axon branching through the Wnt/?-catenin pathway in a Drosophila model for intellectual disability. PLoS ONE. 2013;8:e81791 pubmed publisher
    We recently reported that duplication of the E3 ubiquitin ligase HUWE1 results in intellectual disability (ID) in male patients. However, the underlying molecular mechanism remains unknown...
  34. Park S, Lee D, Kim J, Kim B, Na Y, Jo M, et al. Metformin enhances TRAIL-induced apoptosis by Mcl-1 degradation via Mule in colorectal cancer cells. Oncotarget. 2016;7:59503-59518 pubmed publisher
    ..Knockdown of Mule triggered a significant decrease of Mcl-1 polyubiquitination...
  35. Sung M, Porras Yakushi T, Reitsma J, Huber F, Sweredoski M, Hoelz A, et al. A conserved quality-control pathway that mediates degradation of unassembled ribosomal proteins. elife. 2016;5: pubmed publisher
    ..A similar pathway, mediated by the Tom1 homolog Huwe1, restricts accumulation of overexpressed hRpl26 in human cells...
  36. Moortgat S, Berland S, Aukrust I, Maystadt I, Baker L, Benoit V, et al. HUWE1 variants cause dominant X-linked intellectual disability: a clinical study of 21 patients. Eur J Hum Genet. 2018;26:64-74 pubmed publisher
    Whole-gene duplications and missense variants in the HUWE1 gene (NM_031407.6) have been reported in association with intellectual disability (ID)...
  37. Khoronenkova S, Dianov G. The emerging role of Mule and ARF in the regulation of base excision repair. FEBS Lett. 2011;585:2831-5 pubmed publisher
    ..that ARF is induced in response to DNA damage and inhibits, by direct interaction, the E3 ubiquitin ligase Mule that regulates p53 protein levels...
  38. Atsumi Y, Minakawa Y, Ono M, Dobashi S, Shinohe K, Shinohara A, et al. ATM and SIRT6/SNF2H Mediate Transient H2AX Stabilization When DSBs Form by Blocking HUWE1 to Allow Efficient γH2AX Foci Formation. Cell Rep. 2015;13:2728-40 pubmed publisher
    ..Synthesized H2AX ordinarily underwent degradation through poly-ubiquitination mediated by the E3 ligase HUWE1; however, H2AX ubiquitination was transiently halted upon DSB formation...
  39. Zhang C, Peng Z, Zhu M, Wang P, Du X, Li X, et al. USP9X destabilizes pVHL and promotes cell proliferation. Oncotarget. 2016;7:60519-60534 pubmed publisher
    ..Our findings demonstrate that USP9X is a novel regulator of pVHL stability, and USP9X may be a therapeutic target for treatment of VHL-related tumors. ..
  40. Dominguez Brauer C, Khatun R, Elia A, Thu K, Ramachandran P, Baniasadi S, et al. E3 ubiquitin ligase Mule targets β-catenin under conditions of hyperactive Wnt signaling. Proc Natl Acad Sci U S A. 2017;114:E1148-E1157 pubmed publisher
    ..We previously showed that Mule/Huwe1/Arf-BP1 (Mule) controls murine intestinal stem and progenitor cell proliferation by modulating the Wnt pathway via ..
  41. Haq I, Durrani A, Khan M, Mushtaq M, Ahmad I. Study of antimicrobial resistance and physiological biomarkers with special reference to Salmonellosis in diarrheic foals in Punjab, Pakistan. Acta Trop. 2017;176:144-149 pubmed publisher
    ..In the present study, 447 diarrheic foals (235 horse foals, 165 donkey foals and 47 mule foal) were selected from Lahore and Sahiwal districts of Punjab, Pakistan...
  42. Yoon S, Lee Y, Kim J, Chung A, Joo J, Kim C, et al. Over-expression of human UREB1 in colorectal cancer: HECT domain of human UREB1 inhibits the activity of tumor suppressor p53 protein. Biochem Biophys Res Commun. 2005;326:7-17 pubmed
    ..The protein human upstream regulatory element binding protein 1 (hUREB1) is a HECT domain protein whose function is not defined yet...
  43. Zhang X, O Kennedy N, Morton J. Extreme Variation of Nutritional Composition and Osmolality of Commercially Available Carbohydrate Energy Gels. Int J Sport Nutr Exerc Metab. 2015;25:504-9 pubmed publisher
    ..ranges (incorporating 51 flavor variants) from 23 brands (Accelerade, CNP, High5, GU, Hammer, Maxim, Clif, USN, Mule, Multipower, Nectar, Carb- Boom, Power Bar, Lucozade, Shotz, TORQ, Dextro, Kinetica, SiS, Zipvit, Maxifuel, ..
  44. You H, Ge Y, Zhang J, Cao Y, Xing J, Su D, et al. Derlin-1 promotes ubiquitylation and degradation of the epithelial Na+ channel, ENaC. J Cell Sci. 2017;130:1027-1036 pubmed publisher
    ..In addition, HUWE1, an endoplasmic reticulum (ER)-resident E3 ubiquitin ligase, was recruited and promoted K11-linked ..
  45. Poulsen E, Steinhauer C, Lees M, Lauridsen A, Ellgaard L, Hartmann Petersen R. HUWE1 and TRIP12 collaborate in degradation of ubiquitin-fusion proteins and misframed ubiquitin. PLoS ONE. 2012;7:e50548 pubmed publisher
    ..The most significant hits from the screen were the E3 ubiquitin-protein ligase HUWE1, as well as PSMD7 and PSMD14 that encode proteasome subunits...
  46. Wang X, Lu G, Li L, Yi J, Yan K, Wang Y, et al. HUWE1 interacts with BRCA1 and promotes its degradation in the ubiquitin-proteasome pathway. Biochem Biophys Res Commun. 2014;444:549-54 pubmed publisher
    ..E3 ligases are involved to promote BRCA1 for ubiquitination and degradation. Here, we identified HUWE1/Mule/ARF-BP1 as a novel BRCA1-interacting protein involved in the control of BRCA1 protein level...
  47. Chen S, Wang S, Cheng X, Xiao S, Zhu X, Lin F, et al. Isolation and characterization of a distinct duck-origin goose parvovirus causing an outbreak of duckling short beak and dwarfism syndrome in China. Arch Virol. 2016;161:2407-16 pubmed publisher
    Many mule duck and Cherry Valley duck flocks in different duck-producing regions of China have shown signs of an apparently new disease designated "short beak and dwarfism syndrome" (SBDS) since 2015...
  48. Fok K, Bose R, Sheng K, Chang C, Katz Egorov M, Culty M, et al. Huwe1 Regulates the Establishment and Maintenance of Spermatogonia by Suppressing DNA Damage Response. Endocrinology. 2017;158:4000-4016 pubmed publisher
    ..In this study, we report that inactivation of the ubiquitin ligase Huwe1 in male germ cells in mice led to the degeneration of spermatogonia in neonates and resulted in a Sertoli cell-..
  49. Michel M, Swatek K, Hospenthal M, Komander D. Ubiquitin Linkage-Specific Affimers Reveal Insights into K6-Linked Ubiquitin Signaling. Mol Cell. 2017;68:233-246.e5 pubmed publisher
    ..A protocol to enrich K6-ubiquitinated proteins from cells identifies HUWE1 as a main E3 ligase for this chain type, and we show that mitofusin-2 is modified with K6-linked polyubiquitin in ..
  50. Searle K, Rice M, Anderson C, Bishop C, Hobbs N. Asynchronous vegetation phenology enhances winter body condition of a large mobile herbivore. Oecologia. 2015;179:377-91 pubmed publisher
    ..We quantified the benefits mule deer (Odocoileus hemionus) accrue from accessing habitats with asynchronous plant phenology in northwest Colorado ..
  51. Senyilmaz D, Virtue S, Xu X, Tan C, Griffin J, Miller A, et al. Regulation of mitochondrial morphology and function by stearoylation of TFR1. Nature. 2015;525:124-8 pubmed publisher
    ..This leads to reduced ubiquitination of mitofusin via HUWE1, thereby promoting mitochondrial fusion and function...
  52. Sloss O, Topham C, Diez M, Taylor S. Mcl-1 dynamics influence mitotic slippage and death in mitosis. Oncotarget. 2016;7:5176-92 pubmed publisher
    ..However, this degradation does not require the activity of either APC/C-Cdc20, FBW7 or MULE, three separate E3 ubiquitin ligases implicated in targeting Mcl-1 for degradation...
  53. Cheng Y, Tong M, Edge A. Destabilization of Atoh1 by E3 Ubiquitin Ligase Huwe1 and Casein Kinase 1 Is Essential for Normal Sensory Hair Cell Development. J Biol Chem. 2016;291:21096-21109 pubmed
    ..The stability of Atoh1 was regulated by the ubiquitin proteasome system through the action of Huwe1, a HECT-domain, E3 ubiquitin ligase...
  54. Ohtake F, Saeki Y, Ishido S, Kanno J, Tanaka K. The K48-K63 Branched Ubiquitin Chain Regulates NF-?B Signaling. Mol Cell. 2016;64:251-266 pubmed publisher
    ..In response to interleukin-1?, the E3 ubiquitin ligase HUWE1 generates K48 branches on K63 chains formed by TRAF6, yielding K48-K63 branched chains...
  55. François Y, Vignal A, Molette C, Marty Gasset N, Davail S, Liaubet L, et al. Deciphering mechanisms underlying the genetic variation of general production and liver quality traits in the overfed mule duck by pQTL analyses. Genet Sel Evol. 2017;49:38 pubmed publisher
    ..The aim of this study was to analyse the mechanisms that underlie phenotypic quantitative trait loci (QTL) in overfed mule ducks by identifying co-localized proteomic QTL (pQTL)...
  56. Vykoukal J, Sun N, Aguilar Bonavides C, Katayama H, Tanaka I, Fahrmann J, et al. Plasma-derived extracellular vesicle proteins as a source of biomarkers for lung adenocarcinoma. Oncotarget. 2017;8:95466-95480 pubmed publisher
    ..top performing EV-associated proteins that distinguished adenocarcinoma cases from controls, SRGN, TPM3, THBS1 and HUWE1, yielded a combined area under the receiver operating characteristic curve (AUC) of 0.90 (95% CI = 0.76-1)...
  57. Bahrami Motlagh H, Vakilian F, Hassanian Moghaddam H, Pourghorban R. Added value of lung window in detecting drug mules on non-contrast abdominal computed tomography. Radiol Med. 2016;121:472-7 pubmed publisher
    ..The final diagnosis of drug mule was based on the detection of packs in stool examination or surgery...
  58. Forget A, Bihannic L, Cigna S, Lefevre C, Remke M, Barnat M, et al. Shh signaling protects Atoh1 from degradation mediated by the E3 ubiquitin ligase Huwe1 in neural precursors. Dev Cell. 2014;29:649-61 pubmed publisher
    ..that SHH regulates Atoh1 stability by preventing its phosphodependent degradation by the E3 ubiquitin ligase Huwe1. Our results reveal that SHH and Atoh1 contribute to a positive autoregulatory loop promoting neuronal precursor ..
  59. Vaughan L, Tan C, Chapman A, Nonaka D, Mack N, Smith D, et al. HUWE1 ubiquitylates and degrades the RAC activator TIAM1 promoting cell-cell adhesion disassembly, migration, and invasion. Cell Rep. 2015;10:88-102 pubmed publisher
    The E3 ubiquitin ligase HUWE1, deregulated in carcinoma, has been implicated in tumor formation...
  60. Yi J, Lu G, Li L, Wang X, Cao L, Lin M, et al. DNA damage-induced activation of CUL4B targets HUWE1 for proteasomal degradation. Nucleic Acids Res. 2015;43:4579-90 pubmed publisher
    The E3 ubiquitin ligase HUWE1/Mule/ARF-BP1 plays an important role in integrating/coordinating diverse cellular processes such as DNA damage repair and apoptosis...
  61. Jang E, Jang H, Shi P, Popa G, Jeoung M, Galperin E. Spatial control of Shoc2-scaffold-mediated ERK1/2 signaling requires remodeling activity of the ATPase PSMC5. J Cell Sci. 2015;128:4428-41 pubmed publisher
    ..The amplitude of the ERK1/2 signals transduced through the complex is fine-tuned by the HUWE1-mediated ubiquitylation of Shoc2 and its signaling partner RAF-1...
  62. Ma W, Zhao P, Zang L, Zhang K, Liao H, Hu Z. Tumour suppressive function of HUWE1 in thyroid cancer. J Biosci. 2016;41:395-405 pubmed
    b>HUWE1 (the HECT, UBA, and WWE domain-containing protein 1) is an ubiquitin E3 ligase which plays an important role in coordinating diverse cellular processes...
  63. Li C, Yen C, Chen Y, Lee K, Fang C, Zhang X, et al. Characterization of the GNMT-HectH9-PREX2 tripartite relationship in the pathogenesis of hepatocellular carcinoma. Int J Cancer. 2017;140:2284-2297 pubmed publisher
    ..Such interaction enhanced degradation of PREX2 through an E3 ligase HectH9-mediated proteasomal ubiquitination pathway...
  64. Aikens E, Kauffman M, Merkle J, Dwinnell S, Fralick G, Monteith K. The greenscape shapes surfing of resource waves in a large migratory herbivore. Ecol Lett. 2017;20:741-750 pubmed publisher
    ..In a population of migratory mule deer (Odocoileus hemionus), 31% surfed plant phenology in spring as well as a theoretically perfect surfer, and 98% ..
  65. Kang M, Yun H, Lee J. KRIBB11 accelerates Mcl-1 degradation through an HSF1-independent, Mule-dependent pathway in A549 non-small cell lung cancer cells. Biochem Biophys Res Commun. 2017;492:304-309 pubmed publisher
    ..Furthermore, this process was triggered via increase ubiquitination by the E3 ligase, Mule, rather than via de-ubiquitination by USP9X...
  66. Papanikolaou Y, Tsoumakas G, Laliotis M, Markantonatos N, Vlahavas I. Large-scale online semantic indexing of biomedical articles via an ensemble of multi-label classification models. J Biomed Semantics. 2017;8:43 pubmed publisher
    ..Our main contribution is a MUlti-Label Ensemble method (MULE) that incorporates a McNemar statistical significance test in order to validate the combination of the constituent ..
  67. Clague J, Lippman S, Yang H, Hildebrandt M, Ye Y, Lee J, et al. Genetic variation in MicroRNA genes and risk of oral premalignant lesions. Mol Carcinog. 2010;49:183-9 pubmed publisher
  68. Leboucher G, Tsai Y, Yang M, Shaw K, Zhou M, Veenstra T, et al. Stress-induced phosphorylation and proteasomal degradation of mitofusin 2 facilitates mitochondrial fragmentation and apoptosis. Mol Cell. 2012;47:547-57 pubmed publisher
    ..of mitofusin 2 in response to cellular stress leads to recruitment of the ubiquitin ligase (E3) Huwe1/Mule/ARF-BP1/HectH9/E3Histone/Lasu1 to mitofusin 2, with the BH3 domain of Huwe1 implicated in this interaction...
  69. Wang X, Lu G, Li L, Yi J, Yan K, Wang Y, et al. HUWE1 interacts with BRCA1 and promotes its degradation in the ubiquitin-proteasome pathway. Biochem Biophys Res Commun. 2014;444:290-5 pubmed publisher
    ..E3 ligases are involved to promote BRCA1 for ubiquitination and degradation. Here, we identified HUWE1/Mule/ARF-BP1 as a novel BRCA1-interacting protein involved in the control of BRCA1 protein level...
  70. Jacobson A, Macfadden A, Wu Z, Peng J, Liu C. Autoregulation of the 26S proteasome by in situ ubiquitination. Mol Biol Cell. 2014;25:1824-35 pubmed publisher
    ..We propose that in situ ubiquitination of the 26S proteasome regulates its activity, which could function to adjust proteasomal activity in response to the alteration of cellular ubiquitination levels. ..
  71. Jang E, Shi P, Bryant J, Chen J, Dukhande V, Gentry M, et al. HUWE1 is a molecular link controlling RAF-1 activity supported by the Shoc2 scaffold. Mol Cell Biol. 2014;34:3579-93 pubmed publisher
    ..Here we report the identification of the E3 ubiquitin ligase HUWE1 as a binding partner and regulator of Shoc2 function...
  72. Lee C, Yang Y, Chen C, Liu J. Syk-mediated tyrosine phosphorylation of mule promotes TNF-induced JNK activation and cell death. Oncogene. 2016;35:1988-95 pubmed publisher
    ..and cell death by suppressing TRAF2 K63-polyubiquitination; upon TNF stimulation, the suppression is relieved by Mule/ARF-BP1-mediated Miz1 ubiquitination and subsequent degradation. It is not known how Mule is activated by TNF...
  73. Fan L, Peng G, Sahgal N, Fazli L, Gleave M, Zhang Y, et al. Regulation of c-Myc expression by the histone demethylase JMJD1A is essential for prostate cancer cell growth and survival. Oncogene. 2016;35:2441-52 pubmed publisher
    ..In parallel, we found that JMJD1A regulated c-Myc stability, likely by inhibiting HUWE1, an E3 ubiquitin ligase known to target degradation of several substrates including c-Myc...
  74. King B, Boccalatte F, Moran Crusio K, Wolf E, Wang J, Kayembe C, et al. The ubiquitin ligase Huwe1 regulates the maintenance and lymphoid commitment of hematopoietic stem cells. Nat Immunol. 2016;17:1312-1321 pubmed publisher
    ..We identified the ubiquitin ligase Huwe1 as a crucial regulator of HSC function via its post-translational control of the oncoprotein N-myc (encoded by ..
  75. Yang D, Sun B, Zhang X, Cheng D, Yu X, Yan L, et al. Huwe1 Sustains Normal Ovarian Epithelial Cell Transformation and Tumor Growth through the Histone H1.3-H19 Cascade. Cancer Res. 2017;77:4773-4784 pubmed publisher
    ..In contrast to many other E3 ligases, the role of HUWE1 in ovarian cancer where HUWE1 is dysregulated has been unclear...
  76. Fox J, Porter B, Easterwood L, Hildenbrand J, Helie P, Smylie J, et al. Acute hepatic steatosis: a helpful diagnostic feature in metallic phosphide-poisoned horses. J Vet Diagn Invest. 2017;:1040638717746707 pubmed publisher
    ..Laboratory confirmation depends on detecting phosphine gas in gastric contents. Eight horses and a mule were exposed to zinc phosphide used to control prairie dogs on a Wyoming ranch...
  77. Jackson P, Eldridge A, Freed E, Furstenthal L, Hsu J, Kaiser B, et al. The lore of the RINGs: substrate recognition and catalysis by ubiquitin ligases. Trends Cell Biol. 2000;10:429-39 pubmed
    ..The separable substrate-recognition domains of E3 enzymes provides a flexible means of linking a conserved ubiquitylation function to potentially thousands of ubiquitylated substrates in eukaryotic cells. ..
  78. Yamamoto S, Okawa K, Nakano T, Sano K, Ogawa K, Masuda T, et al. Huwe1, a novel cellular interactor of Gag-Pol through integrase binding, negatively influences HIV-1 infectivity. Microbes Infect. 2011;13:339-49 pubmed publisher
    ..In this study, Huwe1, a HECT-type E3 ubiquitin ligase, was identified as a new cellular interactor of human immunodeficiency virus type ..
  79. Sander B, Xu W, Eilers M, Popov N, Lorenz S. A conformational switch regulates the ubiquitin ligase HUWE1. elife. 2017;6: pubmed publisher
    The human ubiquitin ligase HUWE1 has key roles in tumorigenesis, yet it is unkown how its activity is regulated...
  80. Li P, Zhang R, Chen J, Sun D, Lan J, Lin S, et al. Development of a duplex semi-nested PCR assay for detection of classical goose parvovirus and novel goose parvovirus-related virus in sick or dead ducks with short beak and dwarfism syndrome. J Virol Methods. 2017;249:165-169 pubmed publisher
    ..Until now, it remains uncertain whether the Cherry Valley ducks and mule ducks with SBDS are co-infected with classical goose parvovirus (GPV) and NGPV...
  81. Bewley M, Marriott H, Tulone C, Francis S, Mitchell T, Read R, et al. A cardinal role for cathepsin d in co-ordinating the host-mediated apoptosis of macrophages and killing of pneumococci. PLoS Pathog. 2011;7:e1001262 pubmed publisher
    ..As a result of cathepsin D activation, Mcl-1 interacted with its ubiquitin ligase Mule and expression declined...
  82. Choi J, Kim H, Kim K, Lee B, Lu W, An W. Selective requirement of H2B N-Terminal tail for p14ARF-induced chromatin silencing. Nucleic Acids Res. 2011;39:9167-80 pubmed publisher
    ..Our results thus reveal a hitherto unknown role of p14ARF in the regulation of chromatin transcription, as well as molecular mechanisms governing the repressive action of p14ARF. ..
  83. Zhou X, Li T, Feng X, Hsiang E, Xiong Y, Guan K, et al. Targeted polyubiquitylation of RASSF1C by the Mule and SCF?-TrCP ligases in response to DNA damage. Biochem J. 2012;441:227-36 pubmed publisher
    ..Furthermore, RASSF1C degradation is enhanced when cells are exposed to stress signals, such as UV irradiation. Mule, a HECT (homologous with E6-associated protein C-terminus) family E3 ligase, but not SCF?-TrCP [where SCF is Skp1 (..
  84. Markkanen E, van Loon B, Ferrari E, Parsons J, Dianov G, Hubscher U. Regulation of oxidative DNA damage repair by DNA polymerase ? and MutYH by cross-talk of phosphorylation and ubiquitination. Proc Natl Acad Sci U S A. 2012;109:437-42 pubmed publisher
    ..Here, we show that the E3 ligase Mule mediates the degradation of Pol ? and that the control of Pol ? levels by Mule has functional consequences for the ..
  85. Chio I, Sasaki M, Ghazarian D, Moreno J, Done S, Ueda T, et al. TRADD contributes to tumour suppression by regulating ULF-dependent p19Arf ubiquitylation. Nat Cell Biol. 2012;14:625-33 pubmed publisher
    ..Our findings also make an important contribution to the rapidly expanding field of p19(Arf) post-translational regulation. ..
  86. Merkle J, Monteith K, Aikens E, Hayes M, Hersey K, Middleton A, et al. Large herbivores surf waves of green-up during spring. Proc Biol Sci. 2016;283: pubmed publisher
    ..functions parametrized with movement data during the green-up period from two populations each of bighorn sheep, mule deer, elk, moose and bison, totalling 463 individuals monitored 1-3 years from 2004 to 2014...
  87. Annabelle T, Karine R, Marie Dominique B, Karine G, Stéphane D. Pre- and post-prandial expression of genes involved in lipid metabolism at the end of the overfeeding period of mule ducks. Mol Cell Biochem. 2018;438:111-121 pubmed publisher
    ..expression (within 2 h) of fatty acid synthase and acyl CoA synthetase long-chain 1 in liver and muscle of mule ducks in addition with a later peak...
  88. Gu J, Dubner R, Fornace A, Iadarola M. UREB1, a tyrosine phosphorylated nuclear protein, inhibits p53 transactivation. Oncogene. 1995;11:2175-8 pubmed
    Tumor suppressor p53 is a transcription activator that upregulates target genes containing the p53 binding site. UREB1, a DNA binding protein that is tyrosine phosphorylated in vivo, shares a significant homology with the human papilloma ..
  89. Gu J, Ren K, Dubner R, Iadarola M. Cloning of a DNA binding protein that is a tyrosine kinase substrate and recognizes an upstream initiator-like sequence in the promoter of the preprodynorphin gene. Brain Res Mol Brain Res. 1994;24:77-88 pubmed
    ..A unique 310 amino acid protein (UreB1) that specifically bound the URE was cloned from a rat brain cDNA library using the URE-containing oligonucleotide...
  90. Rodrigo Brenni M, Gutierrez E, Hegde R. Cytosolic quality control of mislocalized proteins requires RNF126 recruitment to Bag6. Mol Cell. 2014;55:227-37 pubmed publisher
    ..Bag6-dependent ubiquitination can be recapitulated with purified components, paving the way for mechanistic analyses of downstream steps in this cytosolic quality control pathway...
  91. Katsogiannou M, Andrieu C, Baylot V, Baudot A, Dusetti N, Gayet O, et al. The functional landscape of Hsp27 reveals new cellular processes such as DNA repair and alternative splicing and proposes novel anticancer targets. Mol Cell Proteomics. 2014;13:3585-601 pubmed publisher
    ..Validation of Hsp27 involvement in both processes in human prostate cancer cells supports our system biology-predicted functions and provides new insights into Hsp27 roles in cancer cells. ..
  92. Kristariyanto Y, Choi S, Rehman S, Ritorto M, Campbell D, Morrice N, et al. Assembly and structure of Lys33-linked polyubiquitin reveals distinct conformations. Biochem J. 2015;467:345-52 pubmed publisher
    ..Importantly, our work provides a method to assemble Lys33-linked polyUb that will allow further characterization of this atypical chain type. ..