Gene Symbol: HSPA4
Description: heat shock protein family A (Hsp70) member 4
Alias: APG-2, HEL-S-5a, HS24/P52, HSPH2, hsp70, hsp70RY, heat shock 70 kDa protein 4, epididymis secretory sperm binding protein Li 5a, heat shock 70-related protein APG-2, heat shock 70kD protein 4, heat shock 70kDa protein 4, heat shock protein, 110 kDa, hsp70 RY
Species: human
Products:     HSPA4

Top Publications

  1. Song J, Takeda M, Morimoto R. Bag1-Hsp70 mediates a physiological stress signalling pathway that regulates Raf-1/ERK and cell growth. Nat Cell Biol. 2001;3:276-82 pubmed
    ..Here we show that Bag1, a co-chaperone for heat-shock protein 70 (Hsp70), coordinates signals for cell growth in response to cell stress, by downregulating the activity of Raf-1 kinase...
  2. Kundrat L, Regan L. Identification of residues on Hsp70 and Hsp90 ubiquitinated by the cochaperone CHIP. J Mol Biol. 2010;395:587-94 pubmed publisher
    Molecular chaperones Hsp70 and Hsp90 are in part responsible for maintaining the viability of cells by facilitating the folding and maturation process of many essential client proteins...
  3. Gotoh K, Nonoguchi K, Higashitsuji H, Kaneko Y, Sakurai T, Sumitomo Y, et al. Apg-2 has a chaperone-like activity similar to Hsp110 and is overexpressed in hepatocellular carcinomas. FEBS Lett. 2004;560:19-24 pubmed
    ..Among the 17 genes identified were molecular chaperones, Hsp110, Hsp90B, and Hsp70-1...
  4. Tsai Y, Fishman P, Thakor N, Oyler G. Parkin facilitates the elimination of expanded polyglutamine proteins and leads to preservation of proteasome function. J Biol Chem. 2003;278:22044-55 pubmed
    ..Parkin forms a complex with the expanded polyglutamine protein, heat shock protein 70 (Hsp70) and the proteasome, which may be important for the elimination of the expanded polyglutamine protein...
  5. Young J, Hoogenraad N, Hartl F. Molecular chaperones Hsp90 and Hsp70 deliver preproteins to the mitochondrial import receptor Tom70. Cell. 2003;112:41-50 pubmed
    ..Here, we show that in mammals, the cytosolic chaperones Hsp90 and Hsp70 dock onto a specialized TPR domain in the import receptor Tom70 at the outer mitochondrial membrane...
  6. Mishra A, Godavarthi S, Maheshwari M, Goswami A, Jana N. The ubiquitin ligase E6-AP is induced and recruited to aggresomes in response to proteasome inhibition and may be involved in the ubiquitination of Hsp70-bound misfolded proteins. J Biol Chem. 2009;284:10537-45 pubmed publisher
    ..domain family ubiquitin ligase implicated in Angelman syndrome, interacts with the substrate binding domain of Hsp70/Hsc70 chaperones and promotes the degradation of chaperone bound substrates...
  7. Needham P, Mikoluk K, Dhakarwal P, Khadem S, Snyder A, Subramanya A, et al. The thiazide-sensitive NaCl cotransporter is targeted for chaperone-dependent endoplasmic reticulum-associated degradation. J Biol Chem. 2011;286:43611-21 pubmed publisher
    ..were dispensable for NCC ERAD, NCC ubiquitination and degradation required the activity of Ssa1, a cytoplasmic Hsp70 chaperone...
  8. Padmini E, Lavanya S. HSP70-mediated control of endothelial cell apoptosis during pre-eclampsia. Eur J Obstet Gynecol Reprod Biol. 2011;156:158-64 pubmed publisher
    ..A significant increase in HSP70 (p<0.05), HSF1 (p<0.05), Trx (p<0...
  9. Morishima Y, Wang A, Yu Z, Pratt W, Osawa Y, Lieberman A. CHIP deletion reveals functional redundancy of E3 ligases in promoting degradation of both signaling proteins and expanded glutamine proteins. Hum Mol Genet. 2008;17:3942-52 pubmed publisher
    CHIP (carboxy terminus of Hsc70-interacting protein) an E3 ubiquitin ligase that binds to Hsp70 and Hsp90, promotes degradation of several Hsp90-regulated signaling proteins and disease-causing proteins containing expanded glutamine ..

More Information


  1. Wu C, Lin C, Wu M, Wu K. Induction of HSPA4 and HSPA14 by NBS1 overexpression contributes to NBS1-induced in vitro metastatic and transformation activity. J Biomed Sci. 2011;18:1 pubmed publisher
    ..siRNA mediated knockdown of HSPA4 or HSPA14 decreased the in vitro migration, invasion, and transformation activity in H1299 cells overexpressing ..
  2. Zhou J, Schmid T, Frank R, Brüne B. PI3K/Akt is required for heat shock proteins to protect hypoxia-inducible factor 1alpha from pVHL-independent degradation. J Biol Chem. 2004;279:13506-13 pubmed
    ..Considering that PI3K inhibitors down-regulated heat shock protein 90 (Hsp90) as well as Hsp70 expression and moreover attenuated heat- or hypoxia-induced Hsp70 as well as hypoxia-induced Hsp90 up-regulation ..
  3. Ko H, Bailey R, Smith W, Liu Z, Shin J, Lee Y, et al. CHIP regulates leucine-rich repeat kinase-2 ubiquitination, degradation, and toxicity. Proc Natl Acad Sci U S A. 2009;106:2897-902 pubmed publisher
    ..Here we show that the carboxyl terminus of HSP70-interacting protein (CHIP) binds, ubiquitinates, and promotes the ubiquitin proteasomal degradation of LRRK2...
  4. Kalinowska M, Garncarz W, Pietrowska M, Garrard W, Widlak P. Regulation of the human apoptotic DNase/RNase endonuclease G: involvement of Hsp70 and ATP. Apoptosis. 2005;10:821-30 pubmed
    ..Interestingly, heat shock proteins 70 interact with EndoG and are involved in the regulation of its activity. Purified Hsp70 prevented stimulation of EndoG DNase activity by other nuclear factors in the ATP-dependent manner.
  5. Ammirante M, Rosati A, Arra C, Basile A, Falco A, Festa M, et al. IKK{gamma} protein is a target of BAG3 regulatory activity in human tumor growth. Proc Natl Acad Sci U S A. 2010;107:7497-502 pubmed publisher
    ..Indeed, we demonstrate that BAG3 alters the interaction between HSP70 and IKKgamma, increasing availability of IKKgamma and protecting it from proteasome-dependent degradation; this, ..
  6. Townsend P, Cutress R, Sharp A, Brimmell M, Packham G. BAG-1 prevents stress-induced long-term growth inhibition in breast cancer cells via a chaperone-dependent pathway. Cancer Res. 2003;63:4150-7 pubmed
    ..proteasome binding and COOH-terminal amino acids required for interaction with the chaperone molecules, Hsc70 and Hsp70. Although expression of BAG-1 was unaltered by heat shock, endogenous and overexpressed BAG-1S relocalized from ..
  7. Ehrlich E, Wang T, Luo K, Xiao Z, Niewiadomska A, Martinez T, et al. Regulation of Hsp90 client proteins by a Cullin5-RING E3 ubiquitin ligase. Proc Natl Acad Sci U S A. 2009;106:20330-5 pubmed publisher
    ..The link between Cul5 and Hsp90 client regulation may represent an avenue for cancer drug development. ..
  8. Brinker A, Scheufler C, Von Der Mulbe F, Fleckenstein B, Herrmann C, Jung G, et al. Ligand discrimination by TPR domains. Relevance and selectivity of EEVD-recognition in Hsp70 x Hop x Hsp90 complexes. J Biol Chem. 2002;277:19265-75 pubmed
    Protein-protein interaction modules containing so-called tetratricopeptide repeats (TPRs) mediate the assembly of Hsp70/Hsp90 multi-chaperone complexes...
  9. Dai Q, Qian S, Li H, McDonough H, Borchers C, Huang D, et al. Regulation of the cytoplasmic quality control protein degradation pathway by BAG2. J Biol Chem. 2005;280:38673-81 pubmed
    ..CHIP (carboxyl terminus of Hsp70-interacting protein) is an Hsp70-associated ubiquitin ligase that participates in this process by ubiquitylating ..
  10. Moriwaki Y, Kim Y, Ido Y, Misawa H, Kawashima K, Endo S, et al. L347P PINK1 mutant that fails to bind to Hsp90/Cdc37 chaperones is rapidly degraded in a proteasome-dependent manner. Neurosci Res. 2008;61:43-8 pubmed publisher
    ..These results strongly suggest that Hsp90 and Cdc37 are binding partners of PINK1 which regulate its stability. ..
  11. Gamerdinger M, Hajieva P, Kaya A, Wolfrum U, Hartl F, Behl C. Protein quality control during aging involves recruitment of the macroautophagy pathway by BAG3. EMBO J. 2009;28:889-901 pubmed publisher
    The Hsc/Hsp70 co-chaperones of the BAG (Bcl-2-associated athanogene) protein family are modulators of protein quality control. We examined the specific roles of BAG1 and BAG3 in protein degradation during the aging process...
  12. Ganesan S, Rohde G, Eckermann K, Sroka K, Schaefer M, Dohm C, et al. Mutant SOD1 detoxification mechanisms in intact single cells. Cell Death Differ. 2008;15:312-21 pubmed
    ..Moreover, SOD1 ubiquitination levels differ between proteasomal structures and cytoplasmic material. Hsp70 binding and ubiquitination of wt and mtSOD1 species are highly correlated, demonstrating the coupled upregulation ..
  13. Moore D, West A, Dikeman D, Dawson V, Dawson T. Parkin mediates the degradation-independent ubiquitination of Hsp70. J Neurochem. 2008;105:1806-19 pubmed publisher
    ..Here, we identify the molecular chaperones, Hsp70 and Hsc70, as substrates for parkin...
  14. Lahaye X, Vidy A, Fouquet B, Blondel D. Hsp70 protein positively regulates rabies virus infection. J Virol. 2012;86:4743-51 pubmed publisher
    The Hsp70 chaperone plays a central role in multiple processes within cells, including protein translation, folding, intracellular trafficking, and degradation. This protein is implicated in the replication of numerous viruses...
  15. Kalia S, Lee S, Smith P, Liu L, Crocker S, Thorarinsdottir T, et al. BAG5 inhibits parkin and enhances dopaminergic neuron degeneration. Neuron. 2004;44:931-45 pubmed
    ..that bcl-2-associated athanogene 5 (BAG5), a BAG family member, directly interacts with parkin and the chaperone Hsp70. Within this complex, BAG5 inhibits both parkin E3 ubiquitin ligase activity and Hsp70-mediated refolding of ..
  16. Lee J, Seo T, Yi J, Shin K, Yoo S. CHIP has a protective role against oxidative stress-induced cell death through specific regulation of endonuclease G. Cell Death Dis. 2013;4:e666 pubmed publisher
    ..Under normal conditions Hsp70 mediated the interaction between EndoG and CHIP, downregulating EndoG levels in a Hsp70/proteasome-dependent ..
  17. Doong H, Rizzo K, Fang S, Kulpa V, Weissman A, Kohn E. CAIR-1/BAG-3 abrogates heat shock protein-70 chaperone complex-mediated protein degradation: accumulation of poly-ubiquitinated Hsp90 client proteins. J Biol Chem. 2003;278:28490-500 pubmed
    BAG family proteins are regulatory co-chaperones for heat shock protein (Hsp) 70. Hsp70 facilitates the removal of injured proteins by ubiquitin-mediated proteasomal degradation...
  18. Zhou P, Fernandes N, Dodge I, Reddi A, Rao N, Safran H, et al. ErbB2 degradation mediated by the co-chaperone protein CHIP. J Biol Chem. 2003;278:13829-37 pubmed
    ..Here, we show that ErbB2 serves as an in vitro substrate for the Hsp70/Hsp90-associated U-box ubiquitin ligase CHIP...
  19. Kundrat L, Regan L. Balance between folding and degradation for Hsp90-dependent client proteins: a key role for CHIP. Biochemistry. 2010;49:7428-38 pubmed publisher
    ..The central players are the molecular chaperones Hsp70 and Hsp90, the cochaperone HOP, and ubiquitin ligase, CHIP...
  20. Muller P, Růcková E, Halada P, Coates P, Hrstka R, Lane D, et al. C-terminal phosphorylation of Hsp70 and Hsp90 regulates alternate binding to co-chaperones CHIP and HOP to determine cellular protein folding/degradation balances. Oncogene. 2013;32:3101-10 pubmed publisher
    Heat shock proteins Hsp90 and Hsp70 facilitate protein folding but can also direct proteins for ubiquitin-mediated degradation...
  21. Zhu Y, Cao M, Wang W, Wang W, Ren H, Zhao P, et al. Association of heat-shock protein 70 with lipid rafts is required for Japanese encephalitis virus infection in Huh7 cells. J Gen Virol. 2012;93:61-71 pubmed publisher
    ..In the current study, it was demonstrated that heat-shock protein 70 (HSP70), rather than other members of the HSP70 family, was required for JEV entry into a human cell line...
  22. Kong X, Lin Z, Liang D, Fath D, Sang N, Caro J. Histone deacetylase inhibitors induce VHL and ubiquitin-independent proteasomal degradation of hypoxia-inducible factor 1alpha. Mol Cell Biol. 2006;26:2019-28 pubmed
    ..This degradation pathway involves the enhanced interaction of HIF-1alpha with HSP70 and is secondary to a disruption of the HSP70/HSP90 axis function that appears mediated by the activity of HDAC-6.
  23. Valentinis B, Capobianco A, Esposito F, Bianchi A, Rovere Querini P, Manfredi A, et al. Human recombinant heat shock protein 70 affects the maturation pathways of dendritic cells in vitro and has an in vivo adjuvant activity. J Leukoc Biol. 2008;84:199-206 pubmed publisher
    ..human monocyte-derived dendritic cells (DCs), the signal transduction pathways activated by a human recombinant HSP70 (r)HSP70 purified from eukaryotic cells...
  24. Park J, Kim S, Choi M, Lee J, Oh D, Im S, et al. Class II histone deacetylases play pivotal roles in heat shock protein 90-mediated proteasomal degradation of vascular endothelial growth factor receptors. Biochem Biophys Res Commun. 2008;368:318-22 pubmed publisher
    ..in the binding of heat shock protein (Hsp) 90 to VEGFR1 or VEGFR2, followed by an increase of the binding of Hsp70 to VEGFR1 or VEGFR2. However, we noted no remarkable changes in the binding of Hsp90/Hsp70 to VEGFR3...
  25. Udan Johns M, Bengoechea R, Bell S, Shao J, Diamond M, True H, et al. Prion-like nuclear aggregation of TDP-43 during heat shock is regulated by HSP40/70 chaperones. Hum Mol Genet. 2014;23:157-70 pubmed publisher
    ..TDP-43 is constitutively bound to members of the Hsp40/Hsp70 family, and we found that heat shock-induced TDP-43 aggregation is mediated by the availability of these ..
  26. Dai Q, Zhang C, Wu Y, McDonough H, Whaley R, Godfrey V, et al. CHIP activates HSF1 and confers protection against apoptosis and cellular stress. EMBO J. 2003;22:5446-58 pubmed
    ..depends on heat shock factor 1 (HSF1), which is normally under negative regulatory control by molecular chaperones Hsp70 and Hsp90. In metazoan species, the chaperone system also provides protection against apoptosis...
  27. Peng H, Morishima Y, Jenkins G, Dunbar A, Lau M, Patterson C, et al. Ubiquitylation of neuronal nitric-oxide synthase by CHIP, a chaperone-dependent E3 ligase. J Biol Chem. 2004;279:52970-7 pubmed
    ..The addition of purified hsp70 and hsp40 to this in vitro system greatly enhances the amount of nNOS-ubiquitin conjugates, suggesting that CHIP ..
  28. Rafiee P, Theriot M, Nelson V, Heidemann J, Kanaa Y, Horowitz S, et al. Human esophageal microvascular endothelial cells respond to acidic pH stress by PI3K/AKT and p38 MAPK-regulated induction of Hsp70 and Hsp27. Am J Physiol Cell Physiol. 2006;291:C931-45 pubmed
    ..Acid significantly enhanced phosphorylation of Akt and MAPKs in HEMEC as well as inducing Hsp27 and Hsp70. The PI3K inhibitor LY-294002, and Akt small interfering RNA inhibited Akt activation and Hsp70 expression in ..
  29. Tetzlaff J, Putcha P, Outeiro T, Ivanov A, Berezovska O, Hyman B, et al. CHIP targets toxic alpha-Synuclein oligomers for degradation. J Biol Chem. 2008;283:17962-8 pubmed publisher
    ..Here, we studied the effect of a potent molecular chaperone, CHIP (carboxyl terminus of Hsp70-interacting protein), on alphaSyn oligomerization using a novel bimolecular fluorescence complementation assay...
  30. Di Tommaso L, Destro A, Seok J, Balladore E, Terracciano L, Sangiovanni A, et al. The application of markers (HSP70 GPC3 and GS) in liver biopsies is useful for detection of hepatocellular carcinoma. J Hepatol. 2009;50:746-54 pubmed publisher
    ..We investigated the diagnostic accuracy of a panel of markers (HSP70 GPC3 and GS), previously tested in resection specimens, in a series of liver biopsies of large regenerative ..
  31. Kim H, Song E, Lee Y, Kim E, Lee K. Human Fas-associated factor 1 interacts with heat shock protein 70 and negatively regulates chaperone activity. J Biol Chem. 2005;280:8125-33 pubmed
    ..time-of-flight mass spectrometry, we found that hFAF1 binds to the 70-kDa heat shock protein family (Hsc70/Hsp70)...
  32. Tateishi Y, Kawabe Y, Chiba T, Murata S, Ichikawa K, Murayama A, et al. Ligand-dependent switching of ubiquitin-proteasome pathways for estrogen receptor. EMBO J. 2004;23:4813-23 pubmed
    ..One pathway is necessary for the transactivation of the receptor and the other is involved in the quality control of the receptor. ..
  33. Stankiewicz A, Livingstone A, Mohseni N, Mosser D. Regulation of heat-induced apoptosis by Mcl-1 degradation and its inhibition by Hsp70. Cell Death Differ. 2009;16:638-47 pubmed publisher
    ..Overexpression of Hsp70, which prevents heat-induced Bax activation, stabilized Mcl-1 protein levels in heat-shocked cells...
  34. Luo W, Zhong J, Chang R, Hu H, Pandey A, Semenza G. Hsp70 and CHIP selectively mediate ubiquitination and degradation of hypoxia-inducible factor (HIF)-1alpha but Not HIF-2alpha. J Biol Chem. 2010;285:3651-63 pubmed publisher
    ..Here, we identify Hsp70 and CHIP (carboxyl terminus of Hsc70-interacting protein) as HIF-1alpha-interacting proteins...
  35. Becker T, Hartl F, Wieland F. CD40, an extracellular receptor for binding and uptake of Hsp70-peptide complexes. J Cell Biol. 2002;158:1277-85 pubmed
    ..CD40, a cell surface protein crucial for B cell function and autoimmunity, specifically bind and internalize human Hsp70 with bound peptide...
  36. Gurer C, Cimarelli A, Luban J. Specific incorporation of heat shock protein 70 family members into primate lentiviral virions. J Virol. 2002;76:4666-70 pubmed
    ..cyclophilin A, we probed purified virions with antibodies against heat shock proteins Hsp27, Hsp40, Hsp60, Hsp70, Hsc70, and Hsp90...
  37. Saleh A, Srinivasula S, Balkir L, Robbins P, Alnemri E. Negative regulation of the Apaf-1 apoptosome by Hsp70. Nat Cell Biol. 2000;2:476-83 pubmed
    ..Here we show that the documented anti-apoptotic effect of the principal heat-shock protein, Hsp70, is mediated through its direct association with the caspase-recruitment domain (CARD) of Apaf-1 and through ..
  38. Lüders J, Demand J, Hohfeld J. The ubiquitin-related BAG-1 provides a link between the molecular chaperones Hsc70/Hsp70 and the proteasome. J Biol Chem. 2000;275:4613-7 pubmed
    The BAG-1 protein modulates the chaperone activity of Hsc70 and Hsp70 in the mammalian cytosol and nucleus. Remarkably, BAG-1 possesses a ubiquitin-like domain at its amino terminus, suggesting a link to the ubiquitin/proteasome system...
  39. Nonoguchi K, Itoh K, Xue J, Tokuchi H, Nishiyama H, Kaneko Y, et al. Cloning of human cDNAs for Apg-1 and Apg-2, members of the Hsp110 family, and chromosomal assignment of their genes. Gene. 1999;237:21-8 pubmed
    ..In humans, however, only the Hsp110/105 homolog has been identified as a member, and two cDNAs, Hsp70RY and HS24/p52, potentially encoding proteins structurally similar to, but smaller than, mouse Apg-2 have been ..
  40. Laroia G, Cuesta R, Brewer G, Schneider R. Control of mRNA decay by heat shock-ubiquitin-proteasome pathway. Science. 1999;284:499-502 pubmed
    ..Rapid decay involves AU-rich binding protein AUF1, which complexes with heat shock proteins hsc70-hsp70, translation initiation factor eIF4G, and poly(A) binding protein...
  41. Takayama S, Bimston D, Matsuzawa S, Freeman B, Aime Sempe C, Xie Z, et al. BAG-1 modulates the chaperone activity of Hsp70/Hsc70. EMBO J. 1997;16:4887-96 pubmed
    ..We present evidence that the protein BAG-1 is a potential modulator of the molecular chaperones, Hsp70 and Hsc70...
  42. Johnson B, Schumacher R, Ross E, Toft D. Hop modulates Hsp70/Hsp90 interactions in protein folding. J Biol Chem. 1998;273:3679-86 pubmed
    Hop is a 60-kDa protein characterized by its ability to bind the two chaperones, hsp70 and hsp90. We have tested the function of Hop using an assay for the refolding of denatured firefly luciferase...
  43. Ballinger C, Connell P, Wu Y, Hu Z, Thompson L, Yin L, et al. Identification of CHIP, a novel tetratricopeptide repeat-containing protein that interacts with heat shock proteins and negatively regulates chaperone functions. Mol Cell Biol. 1999;19:4535-45 pubmed
    The chaperone function of the mammalian 70-kDa heat shock proteins Hsc70 and Hsp70 is modulated by physical interactions with four previously identified chaperone cofactors: Hsp40, BAG-1, the Hsc70-interacting protein Hip, and the Hsc70-..
  44. Booth L, Roberts J, Tavallai M, Chuckalovcak J, Stringer D, Koromilas A, et al. [Pemetrexed + Sorafenib] lethality is increased by inhibition of ERBB1/2/3-PI3K-NFκB compensatory survival signaling. Oncotarget. 2016;7:23608-32 pubmed publisher
    ..Sorafenib inhibited HSP90 and HSP70 chaperone ATPase activities and reduced the interactions of chaperones with clients including c-MYC, CDC37 and MCL-..
  45. Tabe Y, Kojima K, Yamamoto S, Sekihara K, Matsushita H, DAVIS R, et al. Ribosomal Biogenesis and Translational Flux Inhibition by the Selective Inhibitor of Nuclear Export (SINE) XPO1 Antagonist KPT-185. PLoS ONE. 2015;10:e0137210 pubmed publisher
    ..g., PIM2, EEF1A1, EEF2, and HSP70) that are part of the translational/transcriptional network regulated by heat shock factor 1...
  46. Qiu Y, Ye X, Hanson P, Zhang H, Zong J, Cho B, et al. Hsp70-1: upregulation via selective phosphorylation of heat shock factor 1 during coxsackieviral infection and promotion of viral replication via the AU-rich element. Cell Mol Life Sci. 2016;73:1067-84 pubmed publisher
    ..The internal ribosomal entry site (IRES) within Hsp70 mRNA allows Hsp70 to be translated cap-independently during CVB3 infection when global cap-dependent translation ..
  47. Xu D, Sun L, Liu S, Zhang L, Yang H. Molecular cloning of hsf1 and hsbp1 cDNAs, and the expression of hsf1, hsbp1 and hsp70 under heat stress in the sea cucumber Apostichopus japonicus. Comp Biochem Physiol B Biochem Mol Biol. 2016;198:1-9 pubmed publisher
    ..Heat shock factor binding protein 1 (HSBP1) and feedback control of heat shock protein 70 (HSP70) are major regulators of the activity of HSF1...
  48. Zhang H, Yang J, Wu S, Gong W, Chen C, Perrett S. Glutathionylation of the Bacterial Hsp70 Chaperone DnaK Provides a Link between Oxidative Stress and the Heat Shock Response. J Biol Chem. 2016;291:6967-81 pubmed publisher
    DnaK is the major bacterial Hsp70, participating in DNA replication, protein folding, and the stress response. DnaK cooperates with the Hsp40 co-chaperone DnaJ and the nucleotide exchange factor GrpE...
  49. Cantanhêde L, da Silva Júnior C, Ito M, Felipin K, Nicolete R, Salcedo J, et al. Further Evidence of an Association between the Presence of Leishmania RNA Virus 1 and the Mucosal Manifestations in Tegumentary Leishmaniasis Patients. PLoS Negl Trop Dis. 2015;9:e0004079 pubmed publisher
    ..The clinical diagnosis were confirmed by PCR by targeting hsp70 and kDNA DNA sequences and the species causing the infection were determined by HSP70 PCR-RFPL...
  50. Mitchell K, Boardman L, Clusella Trullas S, Terblanche J. Effects of nutrient and water restriction on thermal tolerance: A test of mechanisms and hypotheses. Comp Biochem Physiol A Mol Integr Physiol. 2017;212:15-23 pubmed publisher
    ..We measured body condition (body water and lipid content) as well as heat shock protein 70 gene (hsp70) and protein (HSP70) levels...
  51. Jia Y, Jing Q, Niu H, Huang B. Ameliorative effect of vitamin E on hepatic oxidative stress and hypoimmunity induced by high-fat diet in turbot (Scophthalmus maximus). Fish Shellfish Immunol. 2017;67:634-642 pubmed publisher
    ..Furthermore, fish fed with high-fat diet promoted higher expression of heat shock protein (hsp70, hsp90), and inhibited expression of complement component 3 (c3) in the liver and tumor necrosis factor-? (tnf-?), ..
  52. Wu C, Hsu W, Wang C, Liang J, Tsai M, Yen C, et al. A Novel Strategy for TNF-Alpha Production by 2-APB Induced Downregulated SOCE and Upregulated HSP70 in O. tsutsugamushi-Infected Human Macrophages. PLoS ONE. 2016;11:e0159299 pubmed publisher
    ..In addition, a novel role of 2-APB was found in macrophages that causes the upregulation of heat shock protein 70 (HSP70) expression associated with ERK activation; upregulated TNF-? production in the case of knockdown HSP70 was ..
  53. Martins E, Santos R, Bettencourt R. Vibrio diabolicus challenge in Bathymodiolus azoricus populations from Menez Gwen and Lucky Strike hydrothermal vent sites. Fish Shellfish Immunol. 2015;47:962-77 pubmed publisher
    ..Expression of Carcinolectin, Serpin-2, SRCR, IRGs, RTK, TLR2, NF-κB, HSP70 and Ferritin genes was greater in MG than LS mussels...
  54. Chen S, Yin C, Lao T, Liang D, He D, Wang C, et al. AMPK-HDAC5 pathway facilitates nuclear accumulation of HIF-1α and functional activation of HIF-1 by deacetylating Hsp70 in the cytosol. Cell Cycle. 2015;14:2520-36 pubmed publisher
    ..Mechanistically, we show that Hsp70 is a cytosolic substrate of HDAC5; and hyperacetylation renders Hsp70 higher affinity for HIF-1α binding, which ..
  55. Ma J, Li Y, Wu M, Li X. Oxidative stress-mediated p53/p21WAF1/CIP1 pathway may be involved in microcystin-LR-induced cytotoxicity in HepG2 cells. Chemosphere. 2018;194:773-783 pubmed publisher
    ..Biochemical assays revealed that MC-LR exposure altered the protein levels of HSP70 and HSP90, generally inhibited superoxide dismutase and catalase, reduced glutathione content, and increased the ..
  56. Groba S, Guttmann S, Niemietz C, Bernick F, Sauer V, Hachmöller O, et al. Downregulation of hepatic multi-drug resistance protein 1 (MDR1) after copper exposure. Metallomics. 2017;9:1279-1287 pubmed publisher
    ..Intracellular copper levels and the expression of MT1 and HSP70 were increased, whereas the expression of CTR1 was reduced. However, the values normalized after weaning...
  57. Wilhelm S, Henneberg A, Köhler H, Rault M, Richter D, Scheurer M, et al. Does wastewater treatment plant upgrading with activated carbon result in an improvement of fish health?. Aquat Toxicol. 2017;192:184-197 pubmed publisher
    ..parallel, biochemical measurements of glycogen revealed increased energy resources in fish liver and, furthermore, hsp70 levels in livers of exposed rainbow trout and in kidneys of exposed brown trout were lower after than before the ..
  58. Montico B, Lapenta C, Ravo M, Martorelli D, Muraro E, Zeng B, et al. Exploiting a new strategy to induce immunogenic cell death to improve dendritic cell-based vaccines for lymphoma immunotherapy. Oncoimmunology. 2017;6:e1356964 pubmed publisher
    ..cell lines with 9-cis-retinoic acid and IFN? (RA/IFN?) induces early membrane exposure of Calreticulin, HSP70 and 90 together with CD47 down-regulation and enhanced HMGB1 secretion...
  59. Hageman J, van Waarde M, Zylicz A, Walerych D, Kampinga H. The diverse members of the mammalian HSP70 machine show distinct chaperone-like activities. Biochem J. 2011;435:127-42 pubmed publisher
  60. Ren B, Zou G, Huang Y, Xu G, Xu F, He J, et al. Serum levels of HSP70 and other DAMP proteins can aid in patient diagnosis after traumatic injury. Cell Stress Chaperones. 2016;21:677-86 pubmed publisher
    ..In this study, we evaluated the expression of the DAMP heat shock protein 70 (HSP70) in trauma patients with and without secondary infections...
  61. Lichtenauer M, Zimmermann M, Nickl S, Lauten A, Goebel B, Pistulli R, et al. Transient hypoxia leads to increased serum levels of heat shock protein-27, -70 and caspase-cleaved cytokeratin 18. Clin Lab. 2014;60:323-8 pubmed
    ..We have previously shown that elevated levels of heat shock protein-27 (HSP27), -70 (HSP70), and caspase-cleaved cytokeratin 18 (ccCK-18) were found in serum of COPD patients correlating with disease ..
  62. Yang J, Hong Y, Wang W, Wu W, Chi Y, Zong H, et al. HSP70 protects BCL2L12 and BCL2L12A from N-terminal ubiquitination-mediated proteasomal degradation. FEBS Lett. 2009;583:1409-14 pubmed publisher
    ..In addition, HSP70 was identified to interact with BCL2L12 and BCL2L12A and protected them from ubiquitinations and degradations in ..
  63. Blessing N, Brockman A, Chadee D. The E3 ligase CHIP mediates ubiquitination and degradation of mixed-lineage kinase 3. Mol Cell Biol. 2014;34:3132-43 pubmed publisher
    ..CHIP or Hsp70 overexpression promoted endogenous MLK3 ubiquitination and induced a decline in MLK3 protein levels in cells with ..
  64. Khan R, Phulukdaree A, Chuturgoon A. Fumonisin B1 induces oxidative stress in oesophageal (SNO) cancer cells. Toxicon. 2018;141:104-111 pubmed publisher
    ..were quantified using luminometry, gene expression of SOD2 by qPCR and protein expression of SOD2, GPx1, Nrf2 and HSP70 by western blotting...