HCK

Summary

Gene Symbol: HCK
Description: HCK proto-oncogene, Src family tyrosine kinase
Alias: JTK9, p59Hck, p61Hck, tyrosine-protein kinase HCK, hematopoietic cell kinase, hemopoietic cell kinase, p59-HCK/p60-HCK
Species: human
Products:     HCK

Top Publications

  1. Briggs S, Sharkey M, Stevenson M, Smithgall T. SH3-mediated Hck tyrosine kinase activation and fibroblast transformation by the Nef protein of HIV-1. J Biol Chem. 1997;272:17899-902 pubmed
    ..virus-1 (HIV-1) has previously been shown to bind with high affinity and specificity in vitro to the SH3 domain of Hck, a Src family member expressed primarily in myeloid cells...
  2. Hiyoshi M, Suzu S, Yoshidomi Y, Hassan R, Harada H, Sakashita N, et al. Interaction between Hck and HIV-1 Nef negatively regulates cell surface expression of M-CSF receptor. Blood. 2008;111:243-50 pubmed
    Nef is a multifunctional pathogenetic protein of HIV-1, the interaction of which with Hck, a Src tyrosine kinase highly expressed in macrophages, has been shown to be responsible for the development of AIDS...
  3. Tanis K, Veach D, Duewel H, Bornmann W, Koleske A. Two distinct phosphorylation pathways have additive effects on Abl family kinase activation. Mol Cell Biol. 2003;23:3884-96 pubmed
    ..2- and 6.2-fold increases in kinase activity, respectively. We also find that the Src family kinase Hck phosphorylates the Abl and Arg activation loops, leading to an additional twofold stimulation of kinase activity...
  4. Cheng H, Hoxie J, Parks W. The conserved core of human immunodeficiency virus type 1 Nef is essential for association with Lck and for enhanced viral replication in T-lymphocytes. Virology. 1999;264:5-15 pubmed
    ..The Nef proteins from HIV-1 and SIV bind to Lck as well as Hck, Lyn, and Fyn...
  5. Trible R, Emert Sedlak L, Smithgall T. HIV-1 Nef selectively activates Src family kinases Hck, Lyn, and c-Src through direct SH3 domain interaction. J Biol Chem. 2006;281:27029-38 pubmed
    ..We expressed Fgr, Fyn, Hck, Lck, Lyn, and Yes as well as c-Src in yeast and found that each kinase was active and induced growth suppression...
  6. Briggs S, Scholtz B, Jacque J, Swingler S, Stevenson M, Smithgall T. HIV-1 Nef promotes survival of myeloid cells by a Stat3-dependent pathway. J Biol Chem. 2001;276:25605-11 pubmed
    ..Nef binds with high affinity to the SH3 domain of the myeloid-restricted tyrosine kinase Hck in vitro, identifying this Src-related kinase as a possible cellular target for Nef in macrophages...
  7. Ye H, Choi H, Poe J, Smithgall T. Oligomerization is required for HIV-1 Nef-induced activation of the Src family protein-tyrosine kinase, Hck. Biochemistry. 2004;43:15775-84 pubmed
    b>Hck is a member of the Src protein-tyrosine kinase family and is expressed strongly in macrophages, an important HIV target cell...
  8. Emert Sedlak L, Narute P, Shu S, Poe J, Shi H, Yanamala N, et al. Effector kinase coupling enables high-throughput screens for direct HIV-1 Nef antagonists with antiretroviral activity. Chem Biol. 2013;20:82-91 pubmed publisher
    ..Because Nef lacks intrinsic enzymatic activity, we developed an assay that couples Nef to the activation of Hck, a Src family member and Nef effector protein...
  9. Hanna Z, Weng X, Kay D, Poudrier J, Lowell C, Jolicoeur P. The pathogenicity of human immunodeficiency virus (HIV) type 1 Nef in CD4C/HIV transgenic mice is abolished by mutation of its SH3-binding domain, and disease development is delayed in the absence of Hck. J Virol. 2001;75:9378-92 pubmed
    ..We also studied whether Hck, one of the effectors previously found to bind to this PXXP motif of Nef, was involved in disease development...

More Information

Publications74

  1. Emert Sedlak L, Kodama T, Lerner E, Dai W, Foster C, Day B, et al. Chemical library screens targeting an HIV-1 accessory factor/host cell kinase complex identify novel antiretroviral compounds. ACS Chem Biol. 2009;4:939-47 pubmed publisher
    ..assay for inhibitors of Nef function by coupling it to one of its host cell binding partners, the Src-family kinase Hck. Hck activation is dependent upon Nef in this assay, providing a direct readout of Nef activity in vitro...
  2. Grzesiek S, Bax A, Clore G, Gronenborn A, Hu J, Kaufman J, et al. The solution structure of HIV-1 Nef reveals an unexpected fold and permits delineation of the binding surface for the SH3 domain of Hck tyrosine protein kinase. Nat Struct Biol. 1996;3:340-5 pubmed
    ..The binding surface of Nef for the SH3 domain of Hck tyrosine protein kinase has been mapped and reveals a non-contiguous (in terms of amino-acid sequence) interaction ..
  3. Yokoyama N, Miller W. Biochemical properties of the Cdc42-associated tyrosine kinase ACK1. Substrate specificity, authphosphorylation, and interaction with Hck. J Biol Chem. 2003;278:47713-23 pubmed
    ..ACK1 interacts most strongly with the SH3 domains of Src family kinases (Src or Hck) via its C-terminal proline-rich domain...
  4. Verollet C, Zhang Y, Le Cabec V, Mazzolini J, Charrière G, Labrousse A, et al. HIV-1 Nef triggers macrophage fusion in a p61Hck- and protease-dependent manner. J Immunol. 2010;184:7030-9 pubmed publisher
    ..Nef has been shown to activate hematopoietic cell kinase (Hck), a Src tyrosine kinase specifically expressed in phagocytes, through a well-described polyproline-..
  5. Ward A, Monkhouse J, Csar X, Touw I, Bello P. The Src-like tyrosine kinase Hck is activated by granulocyte colony-stimulating factor (G-CSF) and docks to the activated G-CSF receptor. Biochem Biophys Res Commun. 1998;251:117-23 pubmed
    ..The hematopoietic cell kinase (Hck) is a member of the Src-family of kinases known to be expressed in cells of the granulocytic ..
  6. Scholz G, Cartledge K, Dunn A. Hck enhances the adherence of lipopolysaccharide-stimulated macrophages via Cbl and phosphatidylinositol 3-kinase. J Biol Chem. 2000;275:14615-23 pubmed
    ..Immunoprecipitation experiments revealed that Cbl associates with Hck in Bac1.2F5 cells, while expression of an activated form of Hck in Bac1...
  7. Chutiwitoonchai N, Hiyoshi M, Mwimanzi P, Ueno T, Adachi A, Ode H, et al. The identification of a small molecule compound that reduces HIV-1 Nef-mediated viral infectivity enhancement. PLoS ONE. 2011;6:e27696 pubmed publisher
    ..Meanwhile, the overexpression of the SH3 domain of the tyrosine kinase Hck, which binds to a PxxP motif in Nef, also reduced viral infectivity...
  8. Narute P, Smithgall T. Nef alleles from all major HIV-1 clades activate Src-family kinases and enhance HIV-1 replication in an inhibitor-sensitive manner. PLoS ONE. 2012;7:e32561 pubmed publisher
    ..Representative Nef proteins from the major HIV-1 subtypes A1, A2, B, C, F1, F2, G, H, J and K strongly activated Hck and Lyn as well as c-Src to a lesser extent, demonstrating for the first time that Src-family kinase activation is ..
  9. Greenway A, Holloway G, McPhee D, Ellis P, Cornall A, Lidman M. HIV-1 Nef control of cell signalling molecules: multiple strategies to promote virus replication. J Biosci. 2003;28:323-35 pubmed
  10. Suzu S, Harada H, Matsumoto T, Okada S. HIV-1 Nef interferes with M-CSF receptor signaling through Hck activation and inhibits M-CSF bioactivities. Blood. 2005;105:3230-7 pubmed
    HIV-1 Nef protein is a major determinant of the pathogenicity of the virus. It has been shown that Nef activates Hck, a member of Src family kinase, in monocytes/macrophages and that the interaction is critical for AIDS-like disease ..
  11. Dikeakos J, Atkins K, Thomas L, Emert Sedlak L, Byeon I, Jung J, et al. Small molecule inhibition of HIV-1-induced MHC-I down-regulation identifies a temporally regulated switch in Nef action. Mol Biol Cell. 2010;21:3279-92 pubmed publisher
    ..Together, these studies resolve the seemingly controversial models that describe Nef-induced MHC-I down-regulation and provide new insights into the mechanism of Nef action. ..
  12. Hassan R, Suzu S, Hiyoshi M, Takahashi Makise N, Ueno T, Agatsuma T, et al. Dys-regulated activation of a Src tyroine kinase Hck at the Golgi disturbs N-glycosylation of a cytokine receptor Fms. J Cell Physiol. 2009;221:458-68 pubmed publisher
    HIV-1 Nef accelerates the progression to AIDS by binding with and activating a Src kinase Hck, but underlying molecular basis is not understood...
  13. Carreno S, Gouze M, Schaak S, Emorine L, Maridonneau Parini I. Lack of palmitoylation redirects p59Hck from the plasma membrane to p61Hck-positive lysosomes. J Biol Chem. 2000;275:36223-9 pubmed
    b>Hck, a protein-tyrosine kinase of phagocytes, is the unique member of the Src family expressed under two alternatively translated isoforms differing in their N-terminal site of acylation: p61(Hck) has an additional 21-amino acid sequence ..
  14. Briggs S, Lerner E, Smithgall T. Affinity of Src family kinase SH3 domains for HIV Nef in vitro does not predict kinase activation by Nef in vivo. Biochemistry. 2000;39:489-95 pubmed
    ..Previous studies have shown that the SH3 domains of Hck and Lyn bind to Nef via proline-rich sequences in vitro, identifying these Src-related kinases as potential targets ..
  15. Hiipakka M, Poikonen K, Saksela K. SH3 domains with high affinity and engineered ligand specificities targeted to HIV-1 Nef. J Mol Biol. 1999;293:1097-106 pubmed
    The avid binding of HIV-1 Nef to the Src homology-3 (SH3) domain of Hck (KD 250 nM) has been shown to involve an interaction between the RT-loop of Hck-SH3 and residues in Nef outside of its prototypic polyproline type II (PPII) helix-..
  16. Breuer S, Schievink S, Schulte A, Blankenfeldt W, Fackler O, Geyer M. Molecular design, functional characterization and structural basis of a protein inhibitor against the HIV-1 pathogenicity factor Nef. PLoS ONE. 2011;6:e20033 pubmed publisher
    ..Structure determination of the Nef-inhibitor complex reveals the molecular basis for binding specificity. These results establish Nef-NI interfaces as promising leads for the development of potent Nef inhibitors. ..
  17. Komuro I, Yokota Y, Yasuda S, Iwamoto A, Kagawa K. CSF-induced and HIV-1-mediated distinct regulation of Hck and C/EBPbeta represent a heterogeneous susceptibility of monocyte-derived macrophages to M-tropic HIV-1 infection. J Exp Med. 2003;198:443-53 pubmed
    ..M-MPhi express a high level of Hck and a large isoform of C/EBPbeta, and HIV-1 infection increases the expression of Hck but not of C/EBPbeta...
  18. Saksela K, Cheng G, Baltimore D. Proline-rich (PxxP) motifs in HIV-1 Nef bind to SH3 domains of a subset of Src kinases and are required for the enhanced growth of Nef+ viruses but not for down-regulation of CD4. EMBO J. 1995;14:484-91 pubmed
    ..Nef PxxP motifs show specific binding to biotinylated SH3 domains of Hck and Lyn, but not to those of other tested Src family kinases or less related proteins...
  19. Quintrell N, Lebo R, Varmus H, Bishop J, Pettenati M, Le Beau M, et al. Identification of a human gene (HCK) that encodes a protein-tyrosine kinase and is expressed in hemopoietic cells. Mol Cell Biol. 1987;7:2267-75 pubmed
    ..We have designated the gene as HCK (hemopoietic cell kinase) because its expression is prominent in the lymphoid and myeloid lineages of hemopoiesis...
  20. Betzi S, Restouin A, Opi S, Arold S, Parrot I, Guerlesquin F, et al. Protein protein interaction inhibition (2P2I) combining high throughput and virtual screening: Application to the HIV-1 Nef protein. Proc Natl Acad Sci U S A. 2007;104:19256-61 pubmed
  21. Lichtenberg U, Quintrell N, Bishop J. Human protein-tyrosine kinase gene HCK: expression and structural analysis of the promoter region. Oncogene. 1992;7:849-58 pubmed
    The vertebrate gene HCK encodes a protein-tyrosine kinase that is closely related to the product of the proto-oncogene SRC...
  22. Kärkkäinen S, Hiipakka M, Wang J, Kleino I, Vähä Jaakkola M, Renkema G, et al. Identification of preferred protein interactions by phage-display of the human Src homology-3 proteome. EMBO Rep. 2006;7:186-91 pubmed
  23. Lerner E, Smithgall T. SH3-dependent stimulation of Src-family kinase autophosphorylation without tail release from the SH2 domain in vivo. Nat Struct Biol. 2002;9:365-9 pubmed
    ..Here we show that SH3-dependent activation of the Src family member Hck by HIV-1 Nef binding or by SH2-kinase linker mutation does not affect tail tyrosine phosphorylation in fibroblasts...
  24. Meuwissen P, Stolp B, Iannucci V, Vermeire J, Naessens E, Saksela K, et al. Identification of a highly conserved valine-glycine-phenylalanine amino acid triplet required for HIV-1 Nef function. Retrovirology. 2012;9:34 pubmed publisher
    ..molecular level, the VGF motif was required for the physical interaction of the adjacent proline-rich motif with Hck. Based on these findings, we propose that this highly conserved three amino acid VGF motif together with the acidic ..
  25. Sicheri F, Moarefi I, Kuriyan J. Crystal structure of the Src family tyrosine kinase Hck. Nature. 1997;385:602-9 pubmed
    The crystal structure of the haematopoietic cell kinase Hck has been determined at 2.6/2.9 A resolution...
  26. Ziegler S, Marth J, Lewis D, Perlmutter R. Novel protein-tyrosine kinase gene (hck) preferentially expressed in cells of hematopoietic origin. Mol Cell Biol. 1987;7:2276-85 pubmed
    ..Here we report the characterization of a novel human protein-tyrosine kinase gene (hck) that is primarily expressed in hematopoietic cells, particularly granulocytes...
  27. Schreiner S, Schiavone A, Smithgall T. Activation of STAT3 by the Src family kinase Hck requires a functional SH3 domain. J Biol Chem. 2002;277:45680-7 pubmed
    ..tyrosine phosphorylation and stimulation of DNA binding activity by five members of the Src kinase family (Src, Hck, Lyn, Fyn, and Fgr). We also observed stable STAT3.Src family kinase complex formation in this system...
  28. Hiipakka M, Huotari P, Manninen A, Renkema G, Saksela K. Inhibition of cellular functions of HIV-1 Nef by artificial SH3 domains. Virology. 2001;286:152-9 pubmed
    ..Studies on binding of HIV-1 Nef to the SH3 domain of the Hck tyrosine kinase have indicated an important role for the SH3 RT-loop region in ligand binding...
  29. Moarefi I, Lafevre Bernt M, Sicheri F, Huse M, Lee C, Kuriyan J, et al. Activation of the Src-family tyrosine kinase Hck by SH3 domain displacement. Nature. 1997;385:650-3 pubmed
    The protein Hck is a member of the Src family of non-receptor tyrosine kinases which is preferentially expressed in haematopoietic cells of the myeloid and B-lymphoid lineages...
  30. Lee C, Leung B, Lemmon M, Zheng J, Cowburn D, Kuriyan J, et al. A single amino acid in the SH3 domain of Hck determines its high affinity and specificity in binding to HIV-1 Nef protein. EMBO J. 1995;14:5006-15 pubmed
    We have examined the differential binding of Hck and Fyn to HIV-1 Nef to elucidate the structural basis of SH3 binding affinity and specificity...
  31. Lock P, Ralph S, Stanley E, Boulet I, Ramsay R, Dunn A. Two isoforms of murine hck, generated by utilization of alternative translational initiation codons, exhibit different patterns of subcellular localization. Mol Cell Biol. 1991;11:4363-70 pubmed
    ..Our results indicate that translation of murine p59hck initiates from a CTG codon located 21 codons 5' of an ATG that is utilized to generate p56hck...
  32. Jung J, Byeon I, Ahn J, Gronenborn A. Structure, dynamics, and Hck interaction of full-length HIV-1 Nef. Proteins. 2011;79:1609-22 pubmed publisher
    ..of an NMR structural investigation on full-length Nef and its interaction with the entire regulatory domain of Hck (residues 72-256; Hck32L)...
  33. Shivakrupa R, Radha V, Sudhakar C, Swarup G. Physical and functional interaction between Hck tyrosine kinase and guanine nucleotide exchange factor C3G results in apoptosis, which is independent of C3G catalytic domain. J Biol Chem. 2003;278:52188-94 pubmed
    The hematopoietic cell kinase Hck is a Src family tyrosine kinase expressed in cells of myelomonocytic lineage, B lymphocytes, and embryonic stem cells...
  34. Choi H, Smithgall T. Conserved residues in the HIV-1 Nef hydrophobic pocket are essential for recruitment and activation of the Hck tyrosine kinase. J Mol Biol. 2004;343:1255-68 pubmed
    ..Nef binds to the macrophage-specific Src family member Hck through its SH3 domain, resulting in constitutive kinase activation capable of transforming rodent fibroblasts...
  35. Robbins S, Quintrell N, Bishop J. Myristoylation and differential palmitoylation of the HCK protein-tyrosine kinases govern their attachment to membranes and association with caveolae. Mol Cell Biol. 1995;15:3507-15 pubmed
    ..HCK encodes two versions of a protein-tyrosine kinase, with molecular weights of 59,000 (p59hck) and 61,000 (p61hck). The two proteins arise from a single mRNA by alternative initiations of translation...
  36. Arold S, O Brien R, Franken P, Strub M, Hoh F, Dumas C, et al. RT loop flexibility enhances the specificity of Src family SH3 domains for HIV-1 Nef. Biochemistry. 1998;37:14683-91 pubmed
    ..a ligand recognize different SH3 domains? We present the crystal structure of the unbound SH3 domain from hemopoietic cell kinase (Hck) which is a member of the Src family of tyrosine kinases...
  37. Greenway A, Dutartre H, Allen K, McPhee D, Olive D, Collette Y. Simian immunodeficiency virus and human immunodeficiency virus type 1 nef proteins show distinct patterns and mechanisms of Src kinase activation. J Virol. 1999;73:6152-8 pubmed
    ..show here that the Nef protein encoded by SIVmac239 interacts with and also activates the human Src kinases Lck and Hck. This is in direct contrast to the inhibitory effect of HIV type 1 (HIV-1) Nef on Lck catalytic activity...
  38. Hung C, Thomas L, Ruby C, Atkins K, Morris N, Knight Z, et al. HIV-1 Nef assembles a Src family kinase-ZAP-70/Syk-PI3K cascade to downregulate cell-surface MHC-I. Cell Host Microbe. 2007;1:121-33 pubmed
    ..The discovery of this Nef-SFK-ZAP-70/Syk-PI3K signaling pathway explains the hierarchal role of the Nef motifs in effecting immunoevasion. ..
  39. Mangino G, Percario Z, Fiorucci G, Vaccari G, Acconcia F, Chiarabelli C, et al. HIV-1 Nef induces proinflammatory state in macrophages through its acidic cluster domain: involvement of TNF alpha receptor associated factor 2. PLoS ONE. 2011;6:e22982 pubmed publisher
  40. Howlett C, Bisson S, Resek M, Tigley A, Robbins S. The proto-oncogene p120(Cbl) is a downstream substrate of the Hck protein-tyrosine kinase. Biochem Biophys Res Commun. 1999;257:129-38 pubmed
    b>Hematopoietic cell kinase (Hck) is a member of the Src-family of protein tyrosine kinases...
  41. Cornall A, Mak J, Greenway A, Tachedjian G. HIV-1 infection of T cells and macrophages are differentially modulated by virion-associated Hck: a Nef-dependent phenomenon. Viruses. 2013;5:2235-52 pubmed publisher
    ..repeat motif (PxxP) of Nef is required for interaction with the SH3 domains of macrophage-specific Src kinase Hck. However, the implication of this interaction for viral replication and infectivity in macrophages and T ..
  42. Dutartre H, Harris M, Olive D, Collette Y. The human immunodeficiency virus type 1 Nef protein binds the Src-related tyrosine kinase Lck SH2 domain through a novel phosphotyrosine independent mechanism. Virology. 1998;247:200-11 pubmed
    ..Indeed, Nef contains a proline-rich motif implicated in the binding to the Src-like tyrosine kinase Hck and also to a Ser/Thr kinase of molecular weight 62 kDa...
  43. Ren Z, Schaefer T. ErbB-2 activates Stat3 alpha in a Src- and JAK2-dependent manner. J Biol Chem. 2002;277:38486-93 pubmed
  44. Liu Y, Bezverbnaya K, Zhao T, Parsons M, Shi M, Treanor B, et al. Involvement of the HCK and FGR src-family kinases in FCRL4-mediated immune regulation. J Immunol. 2015;194:5851-60 pubmed publisher
    ..These tissue-based cells express increased levels of the src-family kinases HCK and FGR...
  45. Ibarrola I, Vossebeld P, Homburg C, Thelen M, Roos D, Verhoeven A. Influence of tyrosine phosphorylation on protein interaction with FcgammaRIIa. Biochim Biophys Acta. 1997;1357:348-58 pubmed
    ..The GST-CT became phosphorylated in the presence of Lyn, Hck and Syk (immunoprecipitated from human neutrophils), but not in the presence of Fgr...
  46. Zou D, Yang X, Tan Y, Wang P, Zhu X, Yang W, et al. Regulation of the hematopoietic cell kinase (HCK) by PML/RAR? and PU.1 in acute promyelocytic leukemia. Leuk Res. 2012;36:219-23 pubmed publisher
    This study investigates the dynamic regulation of human hematopoietic cell kinase (HCK) in acute promyelocytic leukemia (APL) and the underlying molecular mechanisms...
  47. Marcsisin S, Narute P, Emert Sedlak L, Kloczewiak M, Smithgall T, Engen J. On the solution conformation and dynamics of the HIV-1 viral infectivity factor. J Mol Biol. 2011;410:1008-22 pubmed publisher
    ..Vif specifically interacted with the previously identified binding partner Hck and was able to cause kinase activation, suggesting that the Vif studied by HX MS retained a biochemically ..
  48. Lerner E, Trible R, Schiavone A, Hochrein J, Engen J, Smithgall T. Activation of the Src family kinase Hck without SH3-linker release. J Biol Chem. 2005;280:40832-7 pubmed
    ..To address this question, we engineered a high affinity linker (HAL) mutant of the Src family member Hck in which an optimal SH3 ligand was substituted for the natural linker...
  49. Horenkamp F, Breuer S, Schulte A, Lülf S, Weyand M, Saksela K, et al. Conformation of the dileucine-based sorting motif in HIV-1 Nef revealed by intermolecular domain assembly. Traffic. 2011;12:867-77 pubmed publisher
    ..We have determined the crystal structure of Nef, allele SF2, in complex with an engineered SH3 domain of human Hck showing unnaturally tight binding and inhibitory potential toward Nef...
  50. Mehlitz A, Banhart S, Mäurer A, Kaushansky A, Gordus A, Zielecki J, et al. Tarp regulates early Chlamydia-induced host cell survival through interactions with the human adaptor protein SHC1. J Cell Biol. 2010;190:143-57 pubmed publisher
    ..trachomatis-induced cell survival and suggest that Tarp functions as a multivalent phosphorylation-dependent signaling hub that is important during the early phase of chlamydial infection. ..
  51. Pene Dumitrescu T, Peterson L, Donato N, Smithgall T. An inhibitor-resistant mutant of Hck protects CML cells against the antiproliferative and apoptotic effects of the broad-spectrum Src family kinase inhibitor A-419259. Oncogene. 2008;27:7055-69 pubmed publisher
    ..However, the contribution of Hck and other individual SFKs to Bcr-Abl signaling is less clear...
  52. Yokoyama N, deBakker C, Zappacosta F, Huddleston M, Annan R, Ravichandran K, et al. Identification of tyrosine residues on ELMO1 that are phosphorylated by the Src-family kinase Hck. Biochemistry. 2005;44:8841-9 pubmed
    The SH3 and SH2 domains of hematopoietic cell kinase (Hck) play important roles in substrate targeting. To identify new components of Hck signaling pathways, we identified proteins that bind to the SH3 domain of Hck (Scott et al...
  53. Vérollet C, Souriant S, Bonnaud E, Jolicoeur P, Raynaud Messina B, Kinnaer C, et al. HIV-1 reprograms the migration of macrophages. Blood. 2015;125:1611-22 pubmed publisher
    ..that the stability, size, and proteolytic function of podosomes are increased via the phagocyte-specific kinase Hck and Wiskott-Aldrich syndrome protein (WASP), 2 major regulators of podosomes...
  54. Bhattacharjee A, Pal S, Feldman G, Cathcart M. Hck is a key regulator of gene expression in alternatively activated human monocytes. J Biol Chem. 2011;286:36709-23 pubmed publisher
    ..Additional studies identify Hck as the specific Src family member, stimulated by IL-13 and involved in regulating both p38 MAPK activation and p38 ..
  55. Ling L, Zhu T, Lobie P. Src-CrkII-C3G-dependent activation of Rap1 switches growth hormone-stimulated p44/42 MAP kinase and JNK/SAPK activities. J Biol Chem. 2003;278:27301-11 pubmed
    ..In addition, we demonstrated that C3G-Rap1 mediated CrkII enhancement of GH-stimulated JNK/SAPK activity. We have therefore identified a linear JAK2-independent pathway switching GH-stimulated p44/42 MAP kinase and JNK/SAPK activities. ..
  56. Torres E, Rosen M. Contingent phosphorylation/dephosphorylation provides a mechanism of molecular memory in WASP. Mol Cell. 2003;11:1215-27 pubmed
    ..It provides mechanisms to specifically achieve transient or persistent actin remodeling, as well as long-lasting potentiation of actin-based responses to kinases. ..
  57. Schmidt H, Hoffmann S, Tran T, Stoldt M, Stangler T, Wiesehan K, et al. Solution structure of a Hck SH3 domain ligand complex reveals novel interaction modes. J Mol Biol. 2007;365:1517-32 pubmed
    We studied the interaction of hematopoietic cell kinase SH3 domain (HckSH3) with an artificial 12-residue proline-rich peptide PD1 (HSKYPLPPLPSL) identified as high affinity ligand (K(D)=0.2 muM)...
  58. Cougoule C, Carreno S, Castandet J, Labrousse A, Astarie Dequeker C, Poincloux R, et al. Activation of the lysosome-associated p61Hck isoform triggers the biogenesis of podosomes. Traffic. 2005;6:682-94 pubmed
    ..kinase (Hck) is a protein tyrosine kinase of the Src family specifically expressed in phagocytes as two isoforms, p59Hck and p61Hck, present at the plasma membrane and lysosomes, respectively...
  59. Koda Y, Kikuzato K, Mikuni J, Tanaka A, Yuki H, Honma T, et al. Identification of pyrrolo[2,3-d]pyrimidines as potent HCK and FLT3-ITD dual inhibitors. Bioorg Med Chem Lett. 2017;27:4994-4998 pubmed publisher
    ..Compounds with potent activities against HCK and FLT3-ITD were evaluated in viability studies with acute myeloid leukemia cell line MV4-11...
  60. Hassaine G, Courcoul M, Bessou G, Barthalay Y, Picard C, Olive D, et al. The tyrosine kinase Hck is an inhibitor of HIV-1 replication counteracted by the viral vif protein. J Biol Chem. 2001;276:16885-93 pubmed
    ..S-transferase pull-down assay, we identified that Vif binds specifically to the Src homology 3 domain of Hck, a tyrosine kinase from the Src family...
  61. Bae H, Gray J, Li M, Vines L, Kim J, Pestka J. Hematopoietic cell kinase associates with the 40S ribosomal subunit and mediates the ribotoxic stress response to deoxynivalenol in mononuclear phagocytes. Toxicol Sci. 2010;115:444-52 pubmed publisher
    ..macrophage-a response that is dependent on both double-stranded RNA-activated protein kinase (PKR) and hematopoietic cell kinase (Hck)...
  62. Garnovskaya M, Mukhin Y, Vlasova T, Raymond J. Hypertonicity activates Na+/H+ exchange through Janus kinase 2 and calmodulin. J Biol Chem. 2003;278:16908-15 pubmed
  63. Zhang X, Mahmudi Azer S, Connett J, Anthonisen N, He J, Pare P, et al. Association of Hck genetic polymorphisms with gene expression and COPD. Hum Genet. 2007;120:681-90 pubmed
    ..PMN degranulation is associated with degradation of extracellular matrix and tissue damage. Hck is an essential molecule in the signaling pathway regulating PMN degranulation...
  64. Chang A, O Shaughnessy M, Jirik F. Hck SH3 domain-dependent abrogation of Nef-induced class 1 MHC down-regulation. Eur J Immunol. 2001;31:2382-7 pubmed
    ..Here, using two Nef-expressing human cell model systems, we show that a dominant-negative mutant derived from the Hck protein-tyrosine kinase, composed of the Hck N-terminal region, as well as the SH3 and SH2 domains, was able to ..
  65. Yang G, Buhrlage S, Tan L, Liu X, Chen J, Xu L, et al. HCK is a survival determinant transactivated by mutated MYD88, and a direct target of ibrutinib. Blood. 2016;127:3237-52 pubmed publisher
    ..We observed that mutated MYD88 WM and ABC DLBCL cell lines, as well as primary WM cells show enhanced hematopoietic cell kinase (HCK) transcription and activation, and that HCK is activated by interleukin 6 (IL-6)...