GW182

Summary

Gene Symbol: GW182
Description: trinucleotide repeat containing 6A
Alias: CAGH26, GW1, GW182, TNRC6, trinucleotide repeat-containing gene 6A protein, CAG repeat protein 26, CTD-2540M10.1, EDIE, EMSY interactor protein, GW182 autoantigen, glycine-tryptophan protein of 182 kDa
Species: human
Products:     GW182

Top Publications

  1. Yang Z, Jakymiw A, Wood M, Eystathioy T, Rubin R, Fritzler M, et al. GW182 is critical for the stability of GW bodies expressed during the cell cycle and cell proliferation. J Cell Sci. 2004;117:5567-78 pubmed
    ..referred to as GW bodies was initially identified using human autoantibodies to a 182 kDa protein named GW182. GW bodies are small, generally spherical, cytoplasmic domains that vary in number and size in several mammalian ..
  2. Li S, Lian S, Moser J, Fritzler M, Fritzler M, Satoh M, et al. Identification of GW182 and its novel isoform TNGW1 as translational repressors in Ago2-mediated silencing. J Cell Sci. 2008;121:4134-44 pubmed publisher
    ..b>GW182 (also known as TNRC6A), an 182-kDa protein encoded by TNRC6A, is important for this process, although details of ..
  3. Baillat D, Shiekhattar R. Functional dissection of the human TNRC6 (GW182-related) family of proteins. Mol Cell Biol. 2009;29:4144-55 pubmed publisher
    ..The TNRC6 (trinucleotide repeat containing 6) family of proteins have been shown to stably associate with Agos in mammalian ..
  4. Braun J, Huntzinger E, Fauser M, Izaurralde E. GW182 proteins directly recruit cytoplasmic deadenylase complexes to miRNA targets. Mol Cell. 2011;44:120-33 pubmed publisher
    miRNAs are posttranscriptional regulators of gene expression that associate with Argonaute and GW182 proteins to repress translation and/or promote mRNA degradation...
  5. Liu J, Rivas F, Wohlschlegel J, Yates J, Parker R, Hannon G. A role for the P-body component GW182 in microRNA function. Nat Cell Biol. 2005;7:1261-6 pubmed
    ..Here, we show that the Argonaute proteins physically interact with a key P-/GW-body subunit, GW182. Silencing of GW182 delocalizes resident P-/GW-body proteins and impairs the silencing of microRNA reporters...
  6. Lian S, Li S, Abadal G, Pauley B, Fritzler M, Chan E. The C-terminal half of human Ago2 binds to multiple GW-rich regions of GW182 and requires GW182 to mediate silencing. RNA. 2009;15:804-13 pubmed publisher
    ..Ago2 binds miRNA directly and is the core component of miRNA-induced silencing complex. GW182 is another important factor in miRNA-mediated silencing, and its interaction with Ago2 is evolutionarily conserved...
  7. Fabian M, Mathonnet G, Sundermeier T, Mathys H, Zipprich J, Svitkin Y, et al. Mammalian miRNA RISC recruits CAF1 and PABP to affect PABP-dependent deadenylation. Mol Cell. 2009;35:868-80 pubmed publisher
    ..Importantly, we present evidence that GW182, a core component of the miRISC, directly interacts with PABP via its C-terminal region and that this interaction ..
  8. Huntzinger E, Braun J, Heimstädt S, Zekri L, Izaurralde E. Two PABPC1-binding sites in GW182 proteins promote miRNA-mediated gene silencing. EMBO J. 2010;29:4146-60 pubmed publisher
    miRNA-mediated gene silencing requires the GW182 proteins, which are characterized by an N-terminal domain that interacts with Argonaute proteins (AGOs), and a C-terminal silencing domain (SD)...
  9. Fabian M, Cieplak M, Frank F, Morita M, Green J, Srikumar T, et al. miRNA-mediated deadenylation is orchestrated by GW182 through two conserved motifs that interact with CCR4-NOT. Nat Struct Mol Biol. 2011;18:1211-7 pubmed publisher
    miRNAs recruit the miRNA-induced silencing complex (miRISC), which includes Argonaute and GW182 as core proteins. GW182 proteins effect translational repression and deadenylation of target mRNAs...

More Information

Publications119 found, 100 shown here

  1. Eystathioy T, Jakymiw A, Chan E, Seraphin B, Cougot N, Fritzler M. The GW182 protein colocalizes with mRNA degradation associated proteins hDcp1 and hLSm4 in cytoplasmic GW bodies. RNA. 2003;9:1171-3 pubmed
    ..referred to as GW bodies (GWBs) was initially identified using antibodies specific to a 182-kD protein termed GW182. GW182 was characterized by multiple glycine(G)-tryptophan(W) repeats and an RNA recognition motif (RRM) that ..
  2. Lazzaretti D, Tournier I, Izaurralde E. The C-terminal domains of human TNRC6A, TNRC6B, and TNRC6C silence bound transcripts independently of Argonaute proteins. RNA. 2009;15:1059-66 pubmed publisher
    Proteins of the GW182 family are essential components of the miRNA pathway in animal cells. Vertebrate genomes encode three GW182 paralogs (TNRC6A, TNRC6B, and TNRC6C), which may be functionally redundant...
  3. Eystathioy T, Chan E, Tenenbaum S, Keene J, Griffith K, Fritzler M. A phosphorylated cytoplasmic autoantigen, GW182, associates with a unique population of human mRNAs within novel cytoplasmic speckles. Mol Biol Cell. 2002;13:1338-51 pubmed
    ..The mRNAs associated with GW182 represent a clustered set of transcripts that are presumed to reside within the GW complexes...
  4. Sen G, Blau H. Argonaute 2/RISC resides in sites of mammalian mRNA decay known as cytoplasmic bodies. Nat Cell Biol. 2005;7:633-6 pubmed
    ..In addition, Argonaute 1, another Argonaute family member, is concentrated in cytoplasmic bodies. These results provide new insight into the mechanism of RNAi function. ..
  5. Rehwinkel J, Behm Ansmant I, Gatfield D, Izaurralde E. A crucial role for GW182 and the DCP1:DCP2 decapping complex in miRNA-mediated gene silencing. RNA. 2005;11:1640-7 pubmed
    ..enzymes are found in discrete cytoplasmic foci known as P-bodies or GW-bodies (because of the accumulation of the GW182 antigen). Proteins acting in other post-transcriptional processes have also been localized to P-bodies...
  6. Jakymiw A, Lian S, Eystathioy T, Li S, Satoh M, Hamel J, et al. Disruption of GW bodies impairs mammalian RNA interference. Nat Cell Biol. 2005;7:1267-74 pubmed
    The GW182 RNA-binding protein was initially shown to associate with a specific subset of mRNAs and to reside within discrete cytoplasmic foci named GW bodies (GWBs). GWBs are enriched in proteins that are involved in mRNA degradation...
  7. Eulalio A, Huntzinger E, Izaurralde E. GW182 interaction with Argonaute is essential for miRNA-mediated translational repression and mRNA decay. Nat Struct Mol Biol. 2008;15:346-53 pubmed publisher
    ..Notably, we found these mutations also prevented AGO1 from interacting with GW182 and miRNAs, indicating that the essential role of these residues is unrelated to cap binding...
  8. Yao B, Li S, Jung H, Lian S, Abadal G, Han F, et al. Divergent GW182 functional domains in the regulation of translational silencing. Nucleic Acids Res. 2011;39:2534-47 pubmed publisher
    MicroRNA (miRNA)-mediated gene regulation has become a major focus in many biological processes. GW182 and its long isoform TNGW1 are marker proteins of GW/P bodies and bind to Argonaute proteins of the RNA induced silencing complex...
  9. Chekulaeva M, Mathys H, Zipprich J, Attig J, Colic M, Parker R, et al. miRNA repression involves GW182-mediated recruitment of CCR4-NOT through conserved W-containing motifs. Nat Struct Mol Biol. 2011;18:1218-26 pubmed publisher
    miRNA-mediated repression in animals is dependent on the GW182 protein family...
  10. Pauley K, Eystathioy T, Jakymiw A, Hamel J, Fritzler M, Chan E. Formation of GW bodies is a consequence of microRNA genesis. EMBO Rep. 2006;7:904-10 pubmed
    ..We report that endogenous let-7 miRNA co-precipitates with the GW182 protein complex...
  11. Kozlov G, Safaee N, Rosenauer A, Gehring K. Structural basis of binding of P-body-associated proteins GW182 and ataxin-2 by the Mlle domain of poly(A)-binding protein. J Biol Chem. 2010;285:13599-606 pubmed publisher
    ..Here, we report the crystal structures of the C-terminal Mlle (or PABC) domain in complex with peptides from GW182 (TNRC6C) and Ataxin-2...
  12. Zeng H, He W, Li C, Zhang J, Ling N, Ding Y, et al. Complete genome analysis of a novel phage GW1 lysing Cronobacter. Arch Virol. 2019;164:625-628 pubmed publisher
    A newly identified lytic Cronobacter phage, GW1, was isolated from the Pearl River of Guangzhou, China. GW1 had a double-stranded DNA genome of 39,695 nucleotides with an average GC content of 53.18 %...
  13. Ising H, Kraan T, Rietdijk J, Dragt S, Klaassen R, Boonstra N, et al. Four-Year Follow-up of Cognitive Behavioral Therapy in Persons at Ultra-High Risk for Developing Psychosis: The Dutch Early Detection Intervention Evaluation (EDIE-NL) Trial. Schizophr Bull. 2016;42:1243-52 pubmed publisher
    ..The trial is registered at Current Controlled Trials as trial number ISRCTN21353122 (http://controlled-trials.com/ISRCTN21353122/gaag). ..
  14. Johnston M, Geoffroy M, Sobala A, Hay R, Hutvagner G. HSP90 protein stabilizes unloaded argonaute complexes and microscopic P-bodies in human cells. Mol Biol Cell. 2010;21:1462-9 pubmed publisher
    ..geldanamycin, a well-characterized HSP90 inhibitor, abolishes P-bodies and significantly reduces Argonaute and GW182 protein levels but does not affect the miRNA level and the efficiency of miRNA-mediated gene repression; however, ..
  15. Guess M, Barthel K, Harrison B, Leinwand L. miR-30 family microRNAs regulate myogenic differentiation and provide negative feedback on the microRNA pathway. PLoS ONE. 2015;10:e0118229 pubmed publisher
    ..These findings indicate that the miR-30 family may be an interesting biomarker of perturbed muscle homeostasis and muscle disease. ..
  16. Zhang K, Zhang X, Cai Z, Zhou J, Cao R, Zhao Y, et al. A novel class of microRNA-recognition elements that function only within open reading frames. Nat Struct Mol Biol. 2018;25:1019-1027 pubmed publisher
    ..extensive base-pairing at the 3' side rather than the 5' seed; cause gene silencing in an Argonaute-dependent but GW182-independent manner; and repress translation by inducing transient ribosome stalling instead of mRNA ..
  17. Wu E, Vashisht A, Chapat C, Flamand M, Cohen E, Sarov M, et al. A continuum of mRNP complexes in embryonic microRNA-mediated silencing. Nucleic Acids Res. 2017;45:2081-2098 pubmed publisher
    ..We observed a broad convergence of interactions of germ granule and P body mRNP components on AIN-1/GW182 and NTL-1/CNOT1 in Caenorhabditis elegans embryos...
  18. Zhang M, Xia H, Yu M, Zhu L, Ju L, Chen J, et al. N-acetylcysteine prevents cytotoxic effects induced by man-made mineral fibers in a human bronchial epithelial cell line. Toxicol In Vitro. 2018;53:200-207 pubmed publisher
    ..present study aimed to investigate the cytotoxic effects on human bronchial epithelial cells (BEAS-2B) exposed to GW1, RW1 and RCF2, considering their properties similar to that of asbestos...
  19. Li S, Wang L, Fu B, Dorf M. Trim65: a cofactor for regulation of the microRNA pathway. RNA Biol. 2014;11:1113-21 pubmed publisher
    ..Biochemical studies established that TRIM65 forms stable complexes with TNRC6 proteins and these molecules co-localize in P-body-like structures...
  20. Eystathioy T, Chan E, Takeuchi K, Mahler M, Luft L, Zochodne D, et al. Clinical and serological associations of autoantibodies to GW bodies and a novel cytoplasmic autoantigen GW182. J Mol Med (Berl). 2003;81:811-8 pubmed
    A novel autoantigen named GW182 was recently identified when the serum from a patient with a sensory ataxic polyneuropathy was used to immunoscreen a HeLa cDNA library...
  21. Kuscu C, Kumar P, Kiran M, Su Z, MALIK A, Dutta A. tRNA fragments (tRFs) guide Ago to regulate gene expression post-transcriptionally in a Dicer independent manner. RNA. 2018;: pubmed publisher
    ..Furthermore, tRF-3:target mRNA pairs in the RNA Induced Silencing Complex associate with GW182 proteins, known to repress translation and promote the degradation of target mRNAs...
  22. Zabinsky R, Weum B, Cui M, Han M. RNA Binding Protein Vigilin Collaborates with miRNAs To Regulate Gene Expression for Caenorhabditis elegans Larval Development. G3 (Bethesda). 2017;7:2511-2518 pubmed publisher
    ..causes severe larval arrest at the early L1 stage in animals with compromised miRISC functions (an ain-2/GW182 mutant)...
  23. Niaz S, Hussain M. Role of GW182 protein in the cell. Int J Biochem Cell Biol. 2018;101:29-38 pubmed publisher
    b>GW182 proteins interact directly with the argonaute proteins and constitute key components of miRNA repressor complexes (miRISC) in metazoans...
  24. Zhang R, Jing Y, Zhang H, Niu Y, Liu C, Wang J, et al. Comprehensive Evolutionary Analysis of the Major RNA-Induced Silencing Complex Members. Sci Rep. 2018;8:14189 pubmed publisher
    ..Accumulating evidence has demonstrated that the major RISC members (AGO, DICER, TRBP, PACT and GW182) represent expression discrepancies or multiple orthologues/paralogues in different species...
  25. Sarshad A, Juan A, Muler A, Anastasakis D, Wang X, Genzor P, et al. Argonaute-miRNA Complexes Silence Target mRNAs in the Nucleus of Mammalian Stem Cells. Mol Cell. 2018;71:1040-1050.e8 pubmed publisher
    ..In the nucleus, AGO proteins interact with core RISC components, including the TNRC6 proteins and the CCR4-NOT deadenylase complex...
  26. Schraivogel D, Schindler S, Danner J, Kremmer E, Pfaff J, Hannus S, et al. Importin-β facilitates nuclear import of human GW proteins and balances cytoplasmic gene silencing protein levels. Nucleic Acids Res. 2015;43:7447-61 pubmed publisher
    ..The cytoplasmic functions of TNRC6 and Ago proteins are reasonably well established. Both protein families are found in the nucleus as well...
  27. Krawczynski K, Najmula J, Bauersachs S, Kaczmarek M. MicroRNAome of porcine conceptuses and trophoblasts: expression profile of micrornas and their potential to regulate genes crucial for establishment of pregnancy. Biol Reprod. 2015;92:21 pubmed publisher
    ..Altogether, our results indicate potential roles of these small, noncoding RNAs in the early development of embryos and embryo-maternal cross-talk during early pregnancy in the pig. ..
  28. La Rocca G, Olejniczak S, González A, Briskin D, Vidigal J, Spraggon L, et al. In vivo, Argonaute-bound microRNAs exist predominantly in a reservoir of low molecular weight complexes not associated with mRNA. Proc Natl Acad Sci U S A. 2015;112:767-72 pubmed publisher
    ..Overall, data presented here demonstrate that microRNA-mediated target repression in nontransformed cells depends not only on abundance of specific microRNAs, but also on regulation of RISC assembly by intracellular signaling. ..
  29. Su W, Hong L, Xu X, Huang S, Herpai D, Li L, et al. miR-30 disrupts senescence and promotes cancer by targeting both p16INK4A and DNA damage pathways. Oncogene. 2018;37:5618-5632 pubmed publisher
    ..Thus, the miR-30/CHD7/TNRC6A pathway is potentially a novel diagnostic biomarker and therapeutic target for cancer. ..
  30. Flach C, French P, Dunn G, Fowler D, Gumley A, Birchwood M, et al. Components of therapy as mechanisms of change in cognitive therapy for people at risk of psychosis: analysis of the EDIE-2 trial. Br J Psychiatry. 2015;207:123-9 pubmed publisher
    ..47 to 0.005, P = 0.056). There is a greater treatment effect if formulation and homework are involved in therapy. However, high correlation between components means that these may be indicators of overall treatment fidelity. ..
  31. Morrison A, Birchwood M, Pyle M, Flach C, Stewart S, Byrne R, et al. Impact of cognitive therapy on internalised stigma in people with at-risk mental states. Br J Psychiatry. 2013;203:140-5 pubmed publisher
    ..To investigate the effects of cognitive therapy on internalised stigma using a secondary analysis of data from the EDIE-2 trial...
  32. Fu Y, Chen L, Chen C, Ge Y, Kang M, Song Z, et al. Crosstalk between alternative polyadenylation and miRNA in regulation of protein translational efficiency. Genome Res. 2018;: pubmed publisher
    ..By interfering with the expression of GW182 and analyzing AGO2-PAR-CLIP data, we revealed that the APA effect on translational efficiency was mainly regulated ..
  33. Zielezinski A, Karlowski W. Early origin and adaptive evolution of the GW182 protein family, the key component of RNA silencing in animals. RNA Biol. 2015;12:761-70 pubmed publisher
    The GW182 proteins are a key component of the miRNA-dependent post-transcriptional silencing pathway in animals...
  34. Sahoo M, Gaikwad S, Khuperkar D, Ashok M, Helen M, Yadav S, et al. Nup358 binds to AGO proteins through its SUMO-interacting motifs and promotes the association of target mRNA with miRISC. EMBO Rep. 2017;18:241-263 pubmed publisher
    ..Furthermore, Nup358 interacts with AGO and GW182 proteins and promotes the association of target mRNA with miRISC A well-characterized SUMO-interacting motif (SIM) ..
  35. Yamamoto K, Furukawa M, Fukumura K, Kawamura A, Yamada T, Suzuki H, et al. Control of the heat stress-induced alternative splicing of a subset of genes by hnRNP K. Genes Cells. 2016;21:1006-14 pubmed publisher
    ..Thus, our study clearly showed that several RNA-binding proteins are involved in the splicing regulation in response to heat stress in mammalian cells. ..
  36. Rajgor D, Sanderson T, Amici M, Collingridge G, Hanley J. NMDAR-dependent Argonaute 2 phosphorylation regulates miRNA activity and dendritic spine plasticity. EMBO J. 2018;37: pubmed publisher
    ..transiently increases Akt-dependent phosphorylation of Ago2 at S387, which causes an increase in binding to GW182 and a rapid increase in translational repression of LIMK1 via miR-134...
  37. Eystathioy T, Chan E, Mahler M, Luft L, Fritzler M, Fritzler M. A panel of monoclonal antibodies to cytoplasmic GW bodies and the mRNA binding protein GW182. Hybrid Hybridomics. 2003;22:79-86 pubmed
    b>GW182 is a mRNA binding protein characterized by 60 repeats of glycine (G):tryptophan (W) motifs and is localized in cytoplasmic structures referred to as GW bodies (GWBs)...
  38. Matsui M, Li L, Janowski B, Corey D. Reduced Expression of Argonaute 1, Argonaute 2, and TRBP Changes Levels and Intracellular Distribution of RNAi Factors. Sci Rep. 2015;5:12855 pubmed publisher
    ..AGO1, AGO2, or TRBP expression changed expression levels and nuclear distribution of RNAi factors Dicer, TNRC6A (GW182), and TRBP...
  39. Suzawa M, Noguchi K, Nishi K, Kozuka Hata H, Oyama M, Ui Tei K. Comprehensive Identification of Nuclear and Cytoplasmic TNRC6A-Associating Proteins. J Mol Biol. 2017;429:3319-3333 pubmed publisher
    ..In contrast, pathogen infection- and RNA transport-associated proteins were identified in the cytoplasmic TNRC6A complex. Thus, TNRC6A may be also involved in these pathways in the cytoplasm. ..
  40. Bukhari S, Truesdell S, Lee S, Kollu S, Classon A, Boukhali M, et al. A Specialized Mechanism of Translation Mediated by FXR1a-Associated MicroRNP in Cellular Quiescence. Mol Cell. 2016;61:760-773 pubmed publisher
    ..laevis oocytes by an FXR1a-associated microRNA-protein complex (microRNP) that lacks the microRNP repressor, GW182. Their mechanism in these conditions of decreased mTOR signaling, and therefore reduced canonical (cap-and-poly(A)-..
  41. Jannot G, Michaud P, Quevillon Huberdeau M, Morel Berryman L, Brackbill J, Piquet S, et al. GW182-Free microRNA Silencing Complex Controls Post-transcriptional Gene Expression during Caenorhabditis elegans Embryogenesis. PLoS Genet. 2016;12:e1006484 pubmed publisher
    MicroRNAs and Argonaute form the microRNA induced silencing complex or miRISC that recruits GW182, causing mRNA degradation and/or translational repression...
  42. Bridge K, Shah K, Li Y, Foxler D, Wong S, Miller D, et al. Argonaute Utilization for miRNA Silencing Is Determined by Phosphorylation-Dependent Recruitment of LIM-Domain-Containing Proteins. Cell Rep. 2017;20:173-187 pubmed publisher
    As core components of the microRNA-induced silencing complex (miRISC), Argonaute (AGO) proteins interact with TNRC6 proteins, recruiting other effectors of translational repression/mRNA destabilization...
  43. Bett J, Ibrahim A, Garg A, Kelly V, Pedrioli P, Rocha S, et al. The P-body component USP52/PAN2 is a novel regulator of HIF1A mRNA stability. Biochem J. 2013;451:185-94 pubmed publisher
    ..Importantly, P-body dispersal by knockdown of GW182 or LSM1 resulted in a reduction of HIF1A mRNA levels...
  44. Fukaya T, Iwakawa H, Tomari Y. MicroRNAs block assembly of eIF4F translation initiation complex in Drosophila. Mol Cell. 2014;56:67-78 pubmed publisher
    ..Ago1-RISC associates with a scaffold protein GW182, which recruits additional silencing factors...
  45. Makino S, Mishima Y, Inoue K, Inada T. Roles of mRNA fate modulators Dhh1 and Pat1 in TNRC6-dependent gene silencing recapitulated in yeast. J Biol Chem. 2015;290:8331-47 pubmed publisher
    ..b>GW182/TNRC6 proteins, which are core components of the microRNA-induced silencing complex in animals, stimulate ..
  46. Antic S, Wolfinger M, Skucha A, Hosiner S, Dorner S. General and MicroRNA-Mediated mRNA Degradation Occurs on Ribosome Complexes in Drosophila Cells. Mol Cell Biol. 2015;35:2309-20 pubmed publisher
    ..Also, AGO1 and GW182, two key factors in the miRNA-mediated mRNA degradation pathway, were associated with ribosome complexes...
  47. Reeve S, Nickless A, Sheaves B, Hodgekins J, Stewart S, Gumley A, et al. Sleep duration and psychotic experiences in patients at risk of psychosis: A secondary analysis of the EDIE-2 trial. Schizophr Res. 2019;204:326-333 pubmed publisher
    ..The Early Detection and Intervention Evaluation (EDIE-2) trial provides longitudinal data on sleep duration and individual psychotic experiences from a cohort of ..
  48. Nishi K, Takahashi T, Suzawa M, Miyakawa T, Nagasawa T, Ming Y, et al. Control of the localization and function of a miRNA silencing component TNRC6A by Argonaute protein. Nucleic Acids Res. 2015;43:9856-73 pubmed publisher
    b>GW182 family proteins play important roles in microRNA (miRNA)-mediated RNA silencing. They directly interact with Argonaute (Ago) proteins in processing bodies (P bodies), cytoplasmic foci involved in mRNA degradation and storage...
  49. Kourtidis A, Necela B, Lin W, Lu R, Feathers R, Asmann Y, et al. Cadherin complexes recruit mRNAs and RISC to regulate epithelial cell signaling. J Cell Biol. 2017;216:3073-3085 pubmed publisher
    ..of epithelial adherens junctions recruit the core components of the RNA-induced silencing complex (RISC) Ago2, GW182, and PABPC1, as well as a set of 522 messenger RNAs (mRNAs) and 28 mature microRNAs (miRNAs or miRs), via PLEKHA7...
  50. Xiong X, Vogler G, Kurthkoti K, Samsonova A, Zhou R. SmD1 Modulates the miRNA Pathway Independently of Its Pre-mRNA Splicing Function. PLoS Genet. 2015;11:e1005475 pubmed publisher
    ..Moreover, SmD1 physically and functionally associates with components of the miRISC, including AGO1 and GW182. Notably, miRNA defects resulting from SmD1 silencing can be uncoupled from defects in pre-mRNA splicing, and the ..
  51. Huang S, Liu S, Fu J, Tony Wang T, Yao X, Kumar A, et al. Monocyte Chemotactic Protein-induced Protein 1 and 4 Form a Complex but Act Independently in Regulation of Interleukin-6 mRNA Degradation. J Biol Chem. 2015;290:20782-92 pubmed publisher
    ..Immunofluorescence staining showed that MCPIP4 was co-localized with MCPIP1 in the GW-body, which features GW182 and Argonaute 2...
  52. Mauri M, Kirchner M, Aharoni R, Ciolli Mattioli C, van den Bruck D, Gutkovitch N, et al. Conservation of miRNA-mediated silencing mechanisms across 600 million years of animal evolution. Nucleic Acids Res. 2017;45:938-950 pubmed publisher
    ..miRNA-mediated silencing in bilaterian animals is dependent on the proteins of the GW182 family...
  53. Dittmann K, Mayer C, Czemmel S, Huber S, Rodemann H. New roles for nuclear EGFR in regulating the stability and translation of mRNAs associated with VEGF signaling. PLoS ONE. 2017;12:e0189087 pubmed publisher
    ..This finding argues that an mRNA/miRNA/nEGFR complex regulates protein expression. Indeed, we detected the GW182, AGO2, PABPC1 and cNOT1 proteins, which belong to the deadenylase complex, in a complex with nuclear EGFR...
  54. Ishiura H, Doi K, Mitsui J, Yoshimura J, Matsukawa M, Fujiyama A, et al. Expansions of intronic TTTCA and TTTTA repeats in benign adult familial myoclonic epilepsy. Nat Genet. 2018;50:581-590 pubmed publisher
    ..This discovery that expansions of noncoding repeats lead to neuronal dysfunction responsible for myoclonic tremor and epilepsy extends the understanding of diseases with such repeat expansion. ..
  55. Lewkowicz P, Cwiklińska H, Mycko M, Cichalewska M, Domowicz M, Lewkowicz N, et al. Dysregulated RNA-Induced Silencing Complex (RISC) Assembly within CNS Corresponds with Abnormal miRNA Expression during Autoimmune Demyelination. J Neurosci. 2015;35:7521-37 pubmed publisher
    MicroRNAs (miRNAs) associate with Argonaute (Ago), GW182, and FXR1 proteins to form RNA-induced silencing complexes (RISCs). RISCs represent a critical checkpoint in the regulation and bioavailability of miRNAs...
  56. Mengardi C, Limousin T, Ricci E, Soto Rifo R, Decimo D, Ohlmann T. microRNAs stimulate translation initiation mediated by HCV-like IRESes. Nucleic Acids Res. 2017;45:4810-4824 pubmed publisher
    ..of miRNA-mediated upregulation is unknown, it appears that the poly(A) tail and the lack of availability of the TNRC6 proteins are amongst major determinants...
  57. Bulbrook D, Brazier H, Mahajan P, Kliszczak M, Fedorov O, Marchese F, et al. Tryptophan-Mediated Interactions between Tristetraprolin and the CNOT9 Subunit Are Required for CCR4-NOT Deadenylase Complex Recruitment. J Mol Biol. 2018;430:722-736 pubmed publisher
    ..of interaction with two tryptophan-binding pockets of CNOT9, previously found to interact with another modulator GW182. We further demonstrate that these interactions are also required for recruitment of the CCR4-NOT complex and TTP-..
  58. Pasquale L, Loomis S, Aschard H, Kang J, Cornelis M, Qi L, et al. Exploring genome-wide - dietary heme iron intake interactions and the risk of type 2 diabetes. Front Genet. 2013;4:7 pubmed publisher
    ..72) were significant for the iron metabolic pathway as a whole. We found no significant interactions between dietary heme iron intake and common SNPs in relation to T2D. ..
  59. Krawczynski K, Bauersachs S, Reliszko Z, Graf A, Kaczmarek M. Expression of microRNAs and isomiRs in the porcine endometrium: implications for gene regulation at the maternal-conceptus interface. BMC Genomics. 2015;16:906 pubmed publisher
  60. Olejniczak S, La Rocca G, Radler M, Egan S, Xiang Q, Garippa R, et al. Coordinated Regulation of Cap-Dependent Translation and MicroRNA Function by Convergent Signaling Pathways. Mol Cell Biol. 2016;36:2360-73 pubmed publisher
    ..by cellular activation due in part to induction of the RNA-induced silencing complex (RISC) scaffold protein GW182. In the current study, we demonstrate that increased expression of GW182 in activated or transformed immune cells ..
  61. Cho J, Kim S, Seo Y, Park S, Park B, Kim J, et al. The p90 ribosomal S6 kinase-UBR5 pathway controls Toll-like receptor signaling via miRNA-induced translational inhibition of tumor necrosis factor receptor-associated factor 3. J Biol Chem. 2017;292:11804-11814 pubmed publisher
    ..complex (miRISC), a ribonucleoprotein complex that contains miRNA-engaged Argonaute (Ago) and the scaffold protein GW182. Recently, ubiquitin-protein ligase E3 component N-recognin 5 (UBR5) has been identified as a component of miRISC...
  62. Morrison A, Shryane N, Fowler D, Birchwood M, Gumley A, Taylor H, et al. Negative cognition, affect, metacognition and dimensions of paranoia in people at ultra-high risk of psychosis: a multi-level modelling analysis. Psychol Med. 2015;45:2675-84 pubmed
    ..data from 117 participants from the Early Detection and Intervention Evaluation for people at risk of psychosis (EDIE-2) trial of cognitive–behaviour therapy, comparing them with samples of psychiatric in-patients and healthy ..
  63. Harnisch C, Moritz B, Rammelt C, Temme C, Wahle E. Activity and Function of Deadenylases. Enzymes. 2012;31:181-211 pubmed publisher
    ..This function can be carried out by RNA-binding proteins, for example, members of the PUF family. Alternatively, miRNAs can recruit the deadenylase complexes with the help of their associated GW182 proteins.
  64. Deforzh E, Vargas T, Kropp J, Vandamme M, Pinna G, Polesskaya A. IMP-3 protects the mRNAs of cyclins D1 and D3 from GW182/AGO2-dependent translational repression. Int J Oncol. 2016;49:2578-2588 pubmed publisher
    ..that IMP-3 and its protein partners ILF3/NF90 and PTBP1 bind to the 3'UTRs of the cyclin mRNAs and protect them from the translational repression induced by miRNA-dependent recruitment of AGO2/GW182 complex in human cancer cells.
  65. Patel P, Barbee S, Blankenship J. GW-Bodies and P-Bodies Constitute Two Separate Pools of Sequestered Non-Translating RNAs. PLoS ONE. 2016;11:e0150291 pubmed publisher
    ..Consequently, proteins that govern microRNA-mediated silencing, such as GW182/Gw and AGO1, are often associated with the P-bodies of higher eukaryotic organisms...
  66. Kogure A, Uno M, Ikeda T, Nishida E. The microRNA machinery regulates fasting-induced changes in gene expression and longevity in Caenorhabditis elegans. J Biol Chem. 2017;292:11300-11309 pubmed publisher
    ..up-regulated the expression of the miRNA-induced silencing complex (miRISC) components, including Argonaute and GW182, and the miRNA-processing enzyme DRSH-1 (the ortholog of the Drosophila Drosha enzyme)...
  67. Nieman D, Dragt S, van Duin E, Denneman N, Overbeek J, De Haan L, et al. COMT Val(158)Met genotype and cannabis use in people with an At Risk Mental State for psychosis: Exploring Gene x Environment interactions. Schizophr Res. 2016;174:24-8 pubmed publisher
    ..young adults who met the ARMS criteria and agreed to participate in the Dutch Early Detection and Intervention (EDIE-NL) trial. Cannabis use and COMT Val-allele showed an interaction effect in ARMS subjects...
  68. Li H, Zhang X, Wang F, Zhou L, Yin Z, Fan J, et al. MicroRNA-21 Lowers Blood Pressure in Spontaneous Hypertensive Rats by Upregulating Mitochondrial Translation. Circulation. 2016;134:734-51 pubmed publisher
    ..The direct role of miRNA in mitochondria was determined by GW182 dependence, which is required for miRNA to function in the cytoplasm, but not in mitochondria...
  69. Hicks J, Li L, Matsui M, Chu Y, Volkov O, Johnson K, et al. Human GW182 Paralogs Are the Central Organizers for RNA-Mediated Control of Transcription. Cell Rep. 2017;20:1543-1552 pubmed publisher
    ..Here, we reveal that the human GW182 paralogs TNRC6A/B/C are central organizing factors critical to RNA-mediated transcriptional activation...
  70. Fontenla S, Rinaldi G, Smircich P, Tort J. Conservation and diversification of small RNA pathways within flatworms. BMC Evol Biol. 2017;17:215 pubmed publisher
    ..Similarly, a very divergent GW182 Argonaute interacting protein was identified in all flatworm linages...
  71. Fu Y, Zhang L, Zhang F, Tang T, Zhou Q, Feng C, et al. Exosome-mediated miR-146a transfer suppresses type I interferon response and facilitates EV71 infection. PLoS Pathog. 2017;13:e1006611 pubmed publisher
    ..Moreover, the exosomes contained replication-competent EV71 RNA in complex with miR-146a, Ago2, and GW182 and could mediate EV71 transmission independent of virus-specific receptor...
  72. Guo H, Kazadaeva Y, Ortega F, Manjunath N, Desai T. Trinucleotide repeat containing 6c (TNRC6c) is essential for microvascular maturation during distal airspace sacculation in the developing lung. Dev Biol. 2017;430:214-223 pubmed publisher
    b>GW182 (also known asTNRC6) family members are critically involved in the final effector phase of miRNA-mediated mRNA repression...
  73. Chaston J, Stewart A, Christie M. Structural characterisation of TNRC6A nuclear localisation signal in complex with importin-alpha. PLoS ONE. 2017;12:e0183587 pubmed publisher
    The GW182/TNRC6 family of proteins are central scaffolds that link microRNA-associated Argonaute proteins to the cytoplasmic decay machinery for targeted mRNA degradation processes...
  74. Yao B, La L, Chen Y, Chang L, Chan E. Defining a new role of GW182 in maintaining miRNA stability. EMBO Rep. 2012;13:1102-8 pubmed publisher
    b>GW182 binds to Argonaute (AGO) proteins and has a central role in miRNA-mediated gene silencing. Using lentiviral shRNA-induced GW182 knockdown in HEK293 cells, this study identifies a new role of GW182 in regulating miRNA stability...
  75. Nishi K, Nishi A, Nagasawa T, Ui Tei K. Human TNRC6A is an Argonaute-navigator protein for microRNA-mediated gene silencing in the nucleus. RNA. 2013;19:17-35 pubmed publisher
    b>GW182 family proteins play important roles in microRNA (miRNA)-mediated gene silencing. They interact with Argonaute (Ago) proteins and localize in processing bodies, which are cytoplasmic foci involved in mRNA degradation and storage...
  76. Chan E, Yao B, Fritzler M. Reflections on ten years of history of, and future prospects for, GW182 and GW/P body research. Adv Exp Med Biol. 2013;768:261-70 pubmed publisher
  77. Aqil M, Naqvi A, Bano A, Jameel S. The HIV-1 Nef protein binds argonaute-2 and functions as a viral suppressor of RNA interference. PLoS ONE. 2013;8:e74472 pubmed publisher
    ..The RNA interference (RNAi) pathway proteins Ago2 and GW182 localize to MVBs, suggesting these to be sites for assembly and turnover of the miRNA-induced silencing complex (..
  78. El Shami M, Pontier D, Lahmy S, Braun L, Picart C, Vega D, et al. Reiterated WG/GW motifs form functionally and evolutionarily conserved ARGONAUTE-binding platforms in RNAi-related components. Genes Dev. 2007;21:2539-44 pubmed
    ..Site-specific mutagenesis and domain-swapping experiments between AtNRPD1b and the human protein GW182 demonstrated that reiterated WG/GW motifs form evolutionarily and functionally conserved Argonaute-binding ..
  79. Huntzinger E, Kuzuoğlu Öztürk D, Braun J, Eulalio A, Wohlbold L, Izaurralde E. The interactions of GW182 proteins with PABP and deadenylases are required for both translational repression and degradation of miRNA targets. Nucleic Acids Res. 2013;41:978-94 pubmed publisher
    ..Silencing requires association of miRNAs with an Argonaute protein and a GW182 family protein...
  80. Kraan T, Ising H, Fokkema M, Velthorst E, van den Berg D, Kerkhoven M, et al. The effect of childhood adversity on 4-year outcome in individuals at ultra high risk for psychosis in the Dutch Early Detection Intervention Evaluation (EDIE-NL) Trial. Psychiatry Res. 2017;247:55-62 pubmed publisher
    ..Data pertain to 105 UHR participants of the Dutch Early Detection and Intervention Evaluation (EDIE-NL). Physical abuse was associated with higher depression rates (b=0.381, p=0...
  81. Chen S, Gao G. MicroRNAs recruit eIF4E2 to repress translation of target mRNAs. Protein Cell. 2017;8:750-761 pubmed publisher
    ..We further provide evidence showing that miRNA enhances eIF4E2 association with the target mRNA. We propose that miRNAs recruit eIF4E2 to compete with eIF4E to repress mRNA translation. ..
  82. Liang T, Zhou B, Shi L, Wang H, Chu Q, Xu F, et al. lncRNA AK017368 promotes proliferation and suppresses differentiation of myoblasts in skeletal muscle development by attenuating the function of miR-30c. FASEB J. 2018;32:377-389 pubmed publisher
    ..Wang, H., Chu, Q., Xu, F., Li, Y., Chen, R., Shen, C., Schinckel, A. P. lncRNA AK017368 promotes proliferation and suppresses differentiation of myoblasts in skeletal muscle development by attenuating the function of miR-30c. ..
  83. Margolis R, Abraham M, Gatchell S, Li S, Kidwai A, Breschel T, et al. cDNAs with long CAG trinucleotide repeats from human brain. Hum Genet. 1997;100:114-22 pubmed
    ..These genes are therefore candidates for diseases featuring anticipation, neurodegeneration, or abnormalities of neurodevelopment. ..
  84. Kim M, Oh J, Kim Y, Park S, Kang M, Kim S, et al. Somatic mutations and losses of expression of microRNA regulation-related genes AGO2 and TNRC6A in gastric and colorectal cancers. J Pathol. 2010;221:139-46 pubmed publisher
  85. Yoo N, Hur S, Kim M, Lee J, Lee S. Immunohistochemical analysis of RNA-induced silencing complex-related proteins AGO2 and TNRC6A in prostate and esophageal cancers. APMIS. 2010;118:271-6 pubmed publisher
  86. Bukong T, Hou W, Kodys K, Szabo G. Ethanol facilitates hepatitis C virus replication via up-regulation of GW182 and heat shock protein 90 in human hepatoma cells. Hepatology. 2013;57:70-80 pubmed publisher
    ..However, GW182, a critical component of processing bodies (GW bodies) that is recruited by Ago2 to target messenger RNA (mRNA), ..
  87. Wang X, Chang L, Wang H, Su A, Wu Z. Dcp1a and GW182 Induce Distinct Cellular Aggregates and Have Different Effects on microRNA Pathway. DNA Cell Biol. 2017;36:565-570 pubmed publisher
    ..Here, we aim to investigate different effects of overexpressed Dcp1a or GW182 on cytoplasmic aggregates formation and influence on miRNA pathway...
  88. Cieplak Rotowska M, Tarnowski K, Rubin M, Fabian M, Sonenberg N, Dadlez M, et al. Structural Dynamics of the GW182 Silencing Domain Including its RNA Recognition motif (RRM) Revealed by Hydrogen-Deuterium Exchange Mass Spectrometry. J Am Soc Mass Spectrom. 2018;29:158-173 pubmed publisher
    The human GW182 protein plays an essential role in micro(mi)RNA-dependent gene silencing...
  89. Gibbings D, Leblanc P, Jay F, Pontier D, Michel F, Schwab Y, et al. Human prion protein binds Argonaute and promotes accumulation of microRNA effector complexes. Nat Struct Mol Biol. 2012;19:517-24, S1 pubmed publisher
    ..or stability of miRISC effector complexes containing the trinucleotide repeat-containing gene 6 proteins (TNRC6) and miRNA-repressed mRNA. Accordingly, effective repression of several miRNA targets requires PrP(C)...
  90. Yao B, Li S, Chan E. Function of GW182 and GW bodies in siRNA and miRNA pathways. Adv Exp Med Biol. 2013;768:71-96 pubmed publisher
    b>GW182 is an 182 kDa protein with multiple glycine/tryptophan repeats (GW or WG) playing a central role in siRNA- and miRNA-mediated gene silencing...
  91. Kuzuoğlu Öztürk D, Bhandari D, Huntzinger E, Fauser M, Helms S, Izaurralde E. miRISC and the CCR4-NOT complex silence mRNA targets independently of 43S ribosomal scanning. EMBO J. 2016;35:1186-203 pubmed publisher
    ..A current model for silencing suggests that AGOs mediate these effects through the sequential recruitment of GW182 proteins, the CCR4-NOT deadenylase complex and the translational repressor and decapping activator DDX6...
  92. Yamagata K, Takeda J, Menzel S, Chen X, Eng S, Lim L, et al. Searching for NIDDM susceptibility genes: studies of genes with triplet repeats expressed in skeletal muscle. Diabetologia. 1996;39:725-30 pubmed
    ..Thus, muscle genes with polymorphic CAG/CTG repeats do not appear to play a significant role in the development of NIDDM. ..