GTF2H4

Summary

Gene Symbol: GTF2H4
Description: general transcription factor IIH subunit 4
Alias: P52, TFB2, TFIIH, general transcription factor IIH subunit 4, BTF2 p52, TFIIH basal transcription factor complex p52 subunit, basic transcription factor 2 52 kDa subunit, general transcription factor IIH polypeptide 4, general transcription factor IIH, polypeptide 4, 52kDa
Species: human
Products:     GTF2H4

Top Publications

  1. Yamamoto S, Watanabe Y, van der Spek P, Watanabe T, Fujimoto H, Hanaoka F, et al. Studies of nematode TFIIE function reveal a link between Ser-5 phosphorylation of RNA polymerase II and the transition from transcription initiation to elongation. Mol Cell Biol. 2001;21:1-15 pubmed
    ..However, little is known about its function during these steps. Here we demonstrate for the first time that TFIIH-mediated phosphorylation of RNA polymerase II (Pol II) is essential for the transition to elongation...
  2. Giglia Mari G, Coin F, Ranish J, Hoogstraten D, Theil A, Wijgers N, et al. A new, tenth subunit of TFIIH is responsible for the DNA repair syndrome trichothiodystrophy group A. Nat Genet. 2004;36:714-9 pubmed
    ..TTD) results from mutations in the XPD and XPB subunits of the DNA repair and transcription factor TFIIH. In a third form of DNA repair-deficient TTD, called group A, none of the nine subunits encoding TFIIH carried ..
  3. Jawhari A, Boussert S, Lamour V, Atkinson R, Kieffer B, Poch O, et al. Domain architecture of the p62 subunit from the human transcription/repair factor TFIIH deduced by limited proteolysis and mass spectrometry analysis. Biochemistry. 2004;43:14420-30 pubmed
    b>TFIIH is a multiprotein complex that plays a central role in both transcription and DNA repair...
  4. Lu H, Yu D, Hansen A, Ganguly S, Liu R, Heckert A, et al. Phase-separation mechanism for C-terminal hyperphosphorylation of RNA polymerase II. Nature. 2018;558:318-323 pubmed publisher
    ..T1 droplets, and this process is enhanced upon pre-phosphorylation by CDK7 of transcription initiation factor TFIIH1-3...
  5. Li W, Liu W, Kakoki A, Wang R, Adebali O, Jiang Y, et al. Nucleotide excision repair capacity increases during differentiation of human embryonic carcinoma cells into neurons and muscle cells. J Biol Chem. 2019;294:5914-5922 pubmed publisher
    ..up-regulated during differentiation, but not those of replication protein A (RPA) and transcription factor IIH (TFIIH)...
  6. Lewis B, Burlingame A, Myers S. Human RNA Polymerase II Promoter Recruitment in Vitro Is Regulated by O-Linked N-Acetylglucosaminyltransferase (OGT). J Biol Chem. 2016;291:14056-61 pubmed publisher
    ..and 5 of the pol II C-terminal domain are O-GlcNAcylated, suggesting an overlap with the transcription factor IIH (TFIIH)-dependent serine 5 phosphorylation events during initiation and P-TEFb (positive transcriptional elongation ..
  7. Gao W, Xiao R, Peng B, Xu H, Shen H, Huang M, et al. Arginine methylation of HSP70 regulates retinoid acid-mediated RARβ2 gene activation. Proc Natl Acad Sci U S A. 2015;112:E3327-36 pubmed publisher
    ..R469me1 was shown to mediate the interaction between HSP70 and TFIIH, which involves in RNA polymerase II phosphorylation and thus transcriptional initiation...
  8. Santos D, Alberici L, Hartfelder K. Mitochondrial structure and dynamics as critical factors in honey bee (Apis mellifera L.) caste development. Insect Biochem Mol Biol. 2016;73:1-11 pubmed publisher
    ..Finally, the expression analysis of mitogenesis-related factors showed that the honey bee TFB1 and TFB2 homologs, and a nutritional regulator, ERR, are overexpressed in queen larvae...
  9. Nozawa K, Schneider T, Cramer P. Core Mediator structure at 3.4 Å extends model of transcription initiation complex. Nature. 2017;545:248-251 pubmed publisher
    ..The hook is flexibly linked to Mediator by a conserved hinge and contacts the transcription initiation factor IIH (TFIIH) kinase that phosphorylates the carboxy (C)-terminal domain (CTD) of Pol II and was recently positioned on the PIC...

More Information

Publications153 found, 100 shown here

  1. Yang G, Ibuki Y. Cigarette sidestream smoke delays nucleotide excision repair - inhibited accumulation of repair proteins at DNA lesions. Carcinogenesis. 2017;: pubmed publisher
    ..The recruitment of the repair molecules, TFIIH, XPA, and XPG to pyrimidine dimers was markedly inhibited by CSS...
  2. Liu Y, Mattila J, Ventelä S, Yadav L, Zhang W, Lamichane N, et al. PWP1 Mediates Nutrient-Dependent Growth Control through Nucleolar Regulation of Ribosomal Gene Expression. Dev Cell. 2017;43:240-252.e5 pubmed publisher
    ..data further imply that dPWP1 acts synergistically with mTOR signaling to regulate the nucleolar localization of TFIIH, a known elongation factor of Pol I...
  3. Liu X. In vitro chromatin templates to study nucleotide excision repair. DNA Repair (Amst). 2015;36:68-76 pubmed publisher
    ..been utilized to follow direct removal of DNA damage; (c) six purified human DNA repair factors (RPA, XPA, XPC, TFIIH, XPG, and XPF-ERCC1) that have been used to reconstitute excision repair in vitro...
  4. Sugasawa K. Molecular mechanisms of DNA damage recognition for mammalian nucleotide excision repair. DNA Repair (Amst). 2016;44:110-117 pubmed publisher
    ..Accumulating evidence suggests that XPA and the helicase activity of transcription factor IIH (TFIIH) cooperate to verify abnormalities in DNA chemistry...
  5. Plaschka C, Larivière L, Wenzeck L, Seizl M, Hemann M, Tegunov D, et al. Architecture of the RNA polymerase II-Mediator core initiation complex. Nature. 2015;518:376-80 pubmed publisher
    ..The Mediator 'arm' and 'hook' domains contribute to a 'cradle' that may position the CTD and TFIIH kinase to stimulate Pol II phosphorylation.
  6. Balupillai A, Nagarajan R, Ramasamy K, Govindasamy K, Muthusamy G. Caffeic acid prevents UVB radiation induced photocarcinogenesis through regulation of PTEN signaling in human dermal fibroblasts and mouse skin. Toxicol Appl Pharmacol. 2018;352:87-96 pubmed publisher
    ..AKT kinases through activation of PTEN which subsequently promotes XPC dependant NER proteins such as XPC, XPE, TFIIH (p44) and ERCC1 in HDFa cells and mouse skin tissue...
  7. Hantsche M, Cramer P. Conserved RNA polymerase II initiation complex structure. Curr Opin Struct Biol. 2017;47:17-22 pubmed publisher
    ..The additional factors TFIID, TFIIH, and Mediator have been located on the periphery of the core initiation complex, providing the topology of the ..
  8. Tanaka A, Akimoto Y, Kobayashi S, Hisatake K, Hanaoka F, Ohkuma Y. Association of the winged helix motif of the TFIIEα subunit of TFIIE with either the TFIIEβ subunit or TFIIB distinguishes its functions in transcription. Genes Cells. 2015;20:203-16 pubmed publisher
    ..Furthermore, TFIIEα-WH bound to the TFIIH XPB subunit at a third distinct region...
  9. Posternak V, Ung M, Cheng C, Cole M. MYC Mediates mRNA Cap Methylation of Canonical Wnt/β-Catenin Signaling Transcripts By Recruiting CDK7 and RNA Methyltransferase. Mol Cancer Res. 2017;15:213-224 pubmed publisher
    ..carboxy-terminal domain, mediated in part through an interaction between the TIP60 acetyltransferase complex and TFIIH. MYC enhances mRNA cap methylation above and beyond transcriptional induction. Mol Cancer Res; 15(2); 213-24...
  10. Dienemann C, Schwalb B, Schilbach S, Cramer P. Promoter Distortion and Opening in the RNA Polymerase II Cleft. Mol Cell. 2019;73:97-106.e4 pubmed publisher
    ..promoters that we show can still initiate transcription after depletion of the transcription factor (TF) IIH (TFIIH) translocase Ssl2 (XPB in human) from the nucleus in vivo...
  11. Seifert B, Dejosez M, Zwaka T. Ronin influences the DNA damage response in pluripotent stem cells. Stem Cell Res. 2017;23:98-104 pubmed publisher
    ..Ronin binds to and regulates the genes encoding several DNA repair factors, including Gtf2h4 and Rad18, providing a potential mechanism for this phenotype...
  12. Jun S, Hirata A, Kanai T, Santangelo T, Imanaka T, Murakami K. The X-ray crystal structure of the euryarchaeal RNA polymerase in an open-clamp configuration. Nat Commun. 2014;5:5132 pubmed publisher
    ..to Pol II that correspond to the binding sites of Pol II-specific general transcription factors including TFIIF, TFIIH and Mediator...
  13. Luo S, Horvitz H. The CDK8 Complex and Proneural Proteins Together Drive Neurogenesis from a Mesodermal Lineage. Curr Biol. 2017;27:661-672 pubmed publisher
    ..I4 neurogenesis by inhibiting the CDK-7/CYH-1 (CDK7/cyclin H) kinase module of the transcription initiation factor TFIIH. Ectopic expression of HLH-2 and HLH-3 together promoted expression of neuronal features in non-neuronal cells...
  14. Deitsch E, Hibbard E, Petersen J. The UVS9 gene of Chlamydomonas encodes an XPG homolog with a new conserved domain. DNA Repair (Amst). 2016;37:33-42 pubmed publisher
    ..Despite this difference, amino acid sequence alignment of UVS9p with XPG homologs revealed a new conserved domain involved in TFIIH interaction.
  15. Zhu Q, Wani G, Arab H, El Mahdy M, Ray A, Wani A. Chromatin restoration following nucleotide excision repair involves the incorporation of ubiquitinated H2A at damaged genomic sites. DNA Repair (Amst). 2009;8:262-73 pubmed publisher
    ..CAF-1 p150 was found to associate with NER factors TFIIH, RPA p70 and PCNA in chromatin...
  16. Bao X, Johnson J, Rao H. Rad25 protein is targeted for degradation by the Ubc4-Ufd4 pathway. J Biol Chem. 2015;290:8606-12 pubmed publisher
    ..by the proteasome, we further delineated the specific pathway involved in the degradation of Rad25, a subunit of TFIIH. We demonstrate that Rad25 turnover requires the ubiquitin-conjugating enzyme Ubc4 and the ubiquitin ligase Ufd4...
  17. Hu J, Ye F, Cui M, Lee P, Wei C, Hao Y, et al. Protein Profiling of Bladder Urothelial Cell Carcinoma. PLoS ONE. 2016;11:e0161922 pubmed publisher
    ..Finally, 3 proteins (P2X7, cdc25B and TFIIH p89) were independent factors for favorable overall survival.
  18. Cujec T, Okamoto H, Fujinaga K, Meyer J, Chamberlin H, Morgan D, et al. The HIV transactivator TAT binds to the CDK-activating kinase and activates the phosphorylation of the carboxy-terminal domain of RNA polymerase II. Genes Dev. 1997;11:2645-57 pubmed
    ..which leads to productive interactions between Tat and the CDK-activating kinase (CAK) complex and between Tat and TFIIH. Tat activates the phosphorylation of the carboxy-terminal domain (CTD) of Pol II by CAK in vitro...
  19. Singh A, Compe E, Le May N, Egly J. TFIIH subunit alterations causing xeroderma pigmentosum and trichothiodystrophy specifically disturb several steps during transcription. Am J Hum Genet. 2015;96:194-207 pubmed publisher
    ..the ERCC3 (XPB), ERCC2 (XPD), and GTF2H5 (p8 or TTD-A) subunits of the transcription and DNA-repair factor TFIIH lead to three autosomal-recessive disorders: xeroderma pigmentosum (XP), XP associated with Cockayne syndrome (XP/..
  20. Leveillard T, Andera L, Bissonnette N, Schaeffer L, Bracco L, Egly J, et al. Functional interactions between p53 and the TFIIH complex are affected by tumour-associated mutations. EMBO J. 1996;15:1615-24 pubmed
    ..We show that p53 interacts both physically and functionally with the TFIIH complex...
  21. Murakami K, Gibbons B, Davis R, Nagai S, Liu X, Robinson P, et al. Tfb6, a previously unidentified subunit of the general transcription factor TFIIH, facilitates dissociation of Ssl2 helicase after transcription initiation. Proc Natl Acad Sci U S A. 2012;109:4816-21 pubmed publisher
    General transcription factor TFIIH, previously described as a 10-subunit complex, is essential for transcription and DNA repair...
  22. Ziani S, Nagy Z, Alekseev S, Soutoglou E, Egly J, Coin F. Sequential and ordered assembly of a large DNA repair complex on undamaged chromatin. J Cell Biol. 2014;206:589-98 pubmed publisher
    ..XPC-hHR23b is described as a critical step in the formation of the preincision complex (PInC) further composed of TFIIH, XPA, RPA, XPG, and ERCC1-XPF...
  23. Bucio Mendez A, Cruz Becerra G, Valadez Graham V, Dinkova T, Zurita M. The Dmp8-Dmp18 bicistron messenger RNA enables unusual translation during cellular stress. J Cell Biochem. 2019;120:3887-3897 pubmed publisher
    ..encoded in a bicistronic mRNA next to Dmp8 (Dmp8-Dmp18 transcript), a structural component of transcription factor TFIIH. The organization of these two genes as a bicistron is conserved in all arthropods, however the length of the ..
  24. Zhovmer A, Oksenych V, Coin F. Two sides of the same coin: TFIIH complexes in transcription and DNA repair. ScientificWorldJournal. 2010;10:633-43 pubmed publisher
    b>TFIIH is organized into a seven-subunit core associated with a three-subunit Cdk-activating kinase (CAK) module. TFIIH has roles in both transcription initiation and DNA repair...
  25. Cruz Becerra G, Valerio Cabrera S, Juárez M, Bucio Mendez A, Zurita M. TFIIH localization is highly dynamic during zygotic genome activation in Drosophila, and its depletion causes catastrophic mitosis. J Cell Sci. 2018;131: pubmed publisher
    ..Transcription factor II H (TFIIH) is fundamental for transcription initiation by RNA polymerase II (RNAPII)...
  26. Dembowski J, DeLuca N. Selective recruitment of nuclear factors to productively replicating herpes simplex virus genomes. PLoS Pathog. 2015;11:e1004939 pubmed publisher
    ..RNA polymerase II, the mediator complex, TFIID, TFIIH, and several other transcriptional activators and repressors were also affinity purified with viral DNA...
  27. Warfield L, Luo J, Ranish J, Hahn S. Function of Conserved Topological Regions within the Saccharomyces cerevisiae Basal Transcription Factor TFIIH. Mol Cell Biol. 2016;36:2464-75 pubmed publisher
    b>TFIIH is a 10-subunit RNA polymerase II basal transcription factor with a dual role in DNA repair. TFIIH contains three enzymatic functions and over 30 conserved subdomains and topological regions...
  28. Malik S, Molina H, Xue Z. PIC Activation through Functional Interplay between Mediator and TFIIH. J Mol Biol. 2017;429:48-63 pubmed publisher
    ..Our results reveal a close functional interplay between Mediator and TFIIH in the early stages of PIC development...
  29. Gervais V, Muller I, Mari P, Mourcet A, Movellan K, Ramos P, et al. Small molecule-based targeting of TTD-A dimerization to control TFIIH transcriptional activity represents a potential strategy for anticancer therapy. J Biol Chem. 2018;293:14974-14988 pubmed publisher
    The human transcription factor TFIIH is a large complex composed of 10 subunits that form an intricate network of protein-protein interactions critical for regulating its transcriptional and DNA repair activities...
  30. Herrera Moyano E, Moriel Carretero M, Montelone B, Aguilera A. The rem mutations in the ATP-binding groove of the Rad3/XPD helicase lead to Xeroderma pigmentosum-Cockayne syndrome-like phenotypes. PLoS Genet. 2014;10:e1004859 pubmed publisher
    The eukaryotic TFIIH complex is involved in Nucleotide Excision Repair and transcription initiation. We analyzed three yeast mutations of the Rad3/XPD helicase of TFIIH known as rem (recombination and mutation phenotypes)...
  31. Mu H, Geacintov N, Broyde S, Yeo J, SCHARER O. Molecular basis for damage recognition and verification by XPC-RAD23B and TFIIH in nucleotide excision repair. DNA Repair (Amst). 2018;71:33-42 pubmed publisher
    ..alterations of DNA by the XPC-RAD23B protein and the verification of the damage by the transcription/repair factor TFIIH. Additional factors contribute to the process: UV-DDB, for the recognition of certain UV-induced lesions in ..
  32. Arseni L, Lanzafame M, Compe E, Fortugno P, Afonso Barroso A, Peverali F, et al. TFIIH-dependent MMP-1 overexpression in trichothiodystrophy leads to extracellular matrix alterations in patient skin. Proc Natl Acad Sci U S A. 2015;112:1499-504 pubmed publisher
    Mutations in the XPD subunit of the DNA repair/transcription factor TFIIH result in distinct clinical entities, including the cancer-prone xeroderma pigmentosum (XP) and the multisystem disorder trichothiodystrophy (TTD), which share ..
  33. Akimoto Y, Yamamoto S, Iida S, Hirose Y, Tanaka A, Hanaoka F, et al. Transcription cofactor PC4 plays essential roles in collaboration with the small subunit of general transcription factor TFIIE. Genes Cells. 2014;19:879-90 pubmed publisher
    ..binds to single-stranded DNA and various transcriptional activators, including the general transcription factor TFIIH. In this study, we further investigated PC4 from Homo sapiens and the nematode Caenorhabditis elegans (hPC4 and ..
  34. Fishburn J, Galburt E, Hahn S. Transcription Start Site Scanning and the Requirement for ATP during Transcription Initiation by RNA Polymerase II. J Biol Chem. 2016;291:13040-7 pubmed publisher
    ..that transcription in the purified yeast basal system is largely ATP-independent despite a requirement for the TFIIH DNA translocase subunit Ssl2...
  35. Yang G, Komaki Y, Ibuki Y. Aldehyde-mediated protein degradation is responsible for the inhibition of nucleotide excision repair by cigarette sidestream smoke. Mutat Res. 2018;834:42-50 pubmed publisher
    ..DNA damage, pyrimidine(6-4)pyrimidone photoproducts (6-4PPs), where the recruitments of repair molecules, TFIIH and XPG, were slowed down...
  36. Ribeiro Silva C, Aydin O, Mesquita Ribeiro R, Slyskova J, Helfricht A, Marteijn J, et al. DNA damage sensitivity of SWI/SNF-deficient cells depends on TFIIH subunit p62/GTF2H1. Nat Commun. 2018;9:4067 pubmed publisher
    ..BRM/SMARCA2 and BRG1/SMARCA4 promote the expression of p62/GTF2H1, a core subunit of the transcription factor IIH (TFIIH) complex...
  37. Poss Z, Ebmeier C, Taatjes D. The Mediator complex and transcription regulation. Crit Rev Biochem Mol Biol. 2013;48:575-608 pubmed publisher
    ..within the pre-initiation complex (PIC), which consists of Mediator, pol II, TFIIA, TFIIB, TFIID, TFIIE, TFIIF and TFIIH and is approximately 4.0 MDa in size...
  38. Horibata K, Kono S, Ishigami C, Zhang X, Aizawa M, Kako Y, et al. Constructive rescue of TFIIH instability by an alternative isoform of XPD derived from a mutated XPD allele in mild but not severe XP-D/CS. J Hum Genet. 2015;60:259-65 pubmed publisher
    ..L461V;V716_R730del] by authentic splicing. Additionally, p.[L461V;A717G] could partially rescue the loss of XPD function, resulting in the milder manifestations observed in XP1NE and XPCS118LV. ..
  39. Fishburn J, Tomko E, Galburt E, Hahn S. Double-stranded DNA translocase activity of transcription factor TFIIH and the mechanism of RNA polymerase II open complex formation. Proc Natl Acad Sci U S A. 2015;112:3961-6 pubmed publisher
    ..of the RNA polymerase II (Pol II) open complex (OC) requires DNA unwinding mediated by the transcription factor TFIIH helicase-related subunit XPB/Ssl2...
  40. Okuda M, Kinoshita M, Kakumu E, Sugasawa K, Nishimura Y. Structural Insight into the Mechanism of TFIIH Recognition by the Acidic String of the Nucleotide Excision Repair Factor XPC. Structure. 2015;23:1827-1837 pubmed publisher
    In global genome repair (GGR), XPC detects damaged nucleotides and recruits TFIIH complex...
  41. Rossignol M, Kolb Cheynel I, Egly J. Substrate specificity of the cdk-activating kinase (CAK) is altered upon association with TFIIH. EMBO J. 1997;16:1628-37 pubmed
    The transcription/DNA repair factor TFIIH consists of nine subunits, several exhibiting known functions: helicase/ATPase, kinase activity and DNA binding...
  42. Nilson K, Guo J, Turek M, Brogie J, Delaney E, Luse D, et al. THZ1 Reveals Roles for Cdk7 in Co-transcriptional Capping and Pausing. Mol Cell. 2015;59:576-87 pubmed publisher
    The Cdk7 subunit of TFIIH phosphorylates RNA polymerase II (Pol II) during initiation, and, while recent studies show that inhibition of human Cdk7 negatively influences transcription, the mechanisms involved are unclear...
  43. Petrenko N, Jin Y, Wong K, Struhl K. Mediator Undergoes a Compositional Change during Transcriptional Activation. Mol Cell. 2016;64:443-454 pubmed publisher
    ..with the enhancer, but not with the core promoter, and it dissociates from the enhancer upon depletion of the TFIIH kinase...
  44. Okuda M, Nishimura Y. Extended string binding mode of the phosphorylated transactivation domain of tumor suppressor p53. J Am Chem Soc. 2014;136:14143-52 pubmed publisher
    ..reveal the recognition mechanism of the phosphorylated TAD2 bound to a pleckstrin homology (PH) domain from human TFIIH subunit p62 in an extended string-like conformation...
  45. Andersen S, Zhang A, Dominska M, Moriel Carretero M, Herrera Moyano E, Aguilera A, et al. High-Resolution Mapping of Homologous Recombination Events in rad3 Hyper-Recombination Mutants in Yeast. PLoS Genet. 2016;12:e1005938 pubmed publisher
    The Saccharomyces cerevisae RAD3 gene is the homolog of human XPD, an essential gene encoding a DNA helicase of the TFIIH complex involved in both nucleotide excision repair (NER) and transcription...
  46. Nakatsubo T, Nishitani S, Kikuchi Y, Iida S, Yamada K, Tanaka A, et al. Human mediator subunit MED15 promotes transcriptional activation. Drug Discov Ther. 2014;8:212-7 pubmed
    ..By immunostaining, we observed the colocalization of hMED15 with the general transcription factors TFIIE and TFIIH in the nucleus...
  47. Tajedin L, Anwar M, Gupta D, Tuteja R. Comparative insight into nucleotide excision repair components of Plasmodium falciparum. DNA Repair (Amst). 2015;28:60-72 pubmed publisher
    ..The multi-protein transcription initiation factor TFIIH plays a critical role in NER and transcription and is highly conserved throughout evolution...
  48. Lacombe B, Morel M, Margottin Goguet F, Ramirez B. Specific Inhibition of HIV Infection by the Action of Spironolactone in T Cells. J Virol. 2016;90:10972-10980 pubmed publisher
    ..of HIV Tat-dependent transcription by targeting the general transcription and DNA repair factor II human (TFIIH) has not been reported...
  49. Zhou M, Halanski M, Radonovich M, Kashanchi F, Peng J, Price D, et al. Tat modifies the activity of CDK9 to phosphorylate serine 5 of the RNA polymerase II carboxyl-terminal domain during human immunodeficiency virus type 1 transcription. Mol Cell Biol. 2000;20:5077-86 pubmed
    ..Our results demonstrate that cyclin-dependent kinase 7 (CDK7) (TFIIH) and CDK9 (P-TEFb) both associate with the HIV-1 preinitiation complex...
  50. Galande A, Perween N, Saijo M, Ghaskadbi S, Ghaskadbi S. Analysis of the conserved NER helicases (XPB and XPD) and UV-induced DNA damage in Hydra. Biochim Biophys Acta Gen Subj. 2018;1862:2031-2042 pubmed publisher
    ..Pigmentosum group B (XPB) and group D (XPD) are important helicases in NER and are also critical subunits of TFIIH complex...
  51. Gourdin A, van Cuijk L, Tresini M, Luijsterburg M, Nigg A, Giglia Mari G, et al. Differential binding kinetics of replication protein A during replication and the pre- and post-incision steps of nucleotide excision repair. DNA Repair (Amst). 2014;24:46-56 pubmed publisher
    ..Pre-incision factors like XPC and TFIIH accumulate rapidly at locally induced UV-damage and remain visible up to 4h...
  52. Fan L, DuPrez K. XPB: An unconventional SF2 DNA helicase. Prog Biophys Mol Biol. 2015;117:174-181 pubmed publisher
    ..XPB is an essential core subunit of the eukaryotic basal transcription factor complex TFIIH which plays a dual role in transcription and DNA repair: 1) to facilitate the melting of the promoter during the ..
  53. He Q, Minn I, Wang Q, Xu P, Head S, Datan E, et al. Targeted Delivery and Sustained Antitumor Activity of Triptolide through Glucose Conjugation. Angew Chem Int Ed Engl. 2016;55:12035-9 pubmed publisher
    ..for centuries, has been shown to be an irreversible inhibitor of the XPB subunit of the transcription factor TFIIH and initiation of RNA polymerase?II mediated transcription...
  54. Wood R. DNA damage recognition during nucleotide excision repair in mammalian cells. Biochimie. 1999;81:39-44 pubmed
    ..during nucleotide excision repair (NER) are the XPA protein, the heterotrimeric RPA protein, the 6 to 9-subunit TFIIH, the XPC-hHR23B complex, the XPG nuclease, and the ERCC1-XPF nuclease...
  55. Traboulsi H, Davoli S, Catez P, Egly J, Compe E. Dynamic partnership between TFIIH, PGC-1α and SIRT1 is impaired in trichothiodystrophy. PLoS Genet. 2014;10:e1004732 pubmed publisher
    ..the molecular mechanisms that govern transcription, we focused our attention on the general transcription factor TFIIH, which gives rise, once mutated, to Trichothiodystrophy (TTD), a rare autosomal premature-ageing disease causing ..
  56. Kikuchi Y, Umemura H, Nishitani S, Iida S, Fukasawa R, Hayashi H, et al. Human mediator MED17 subunit plays essential roles in gene regulation by associating with the transcription and DNA repair machineries. Genes Cells. 2015;20:191-202 pubmed publisher
    ..assays showed that hMED17 interacted with Pol II and the general transcription factors TFIIB, TBP, TFIIE, and TFIIH. In addition, hMED17 bound to a DNA helicase subunit of TFIIH, XPB, which is essential for both transcription and ..
  57. Smolarz B, Michalska M, Samulak D, Wojcik L, Romanowicz H. Studies of Correlations Between Single Nucleotide Polymorphisms of DNA Repair Genes and Endometrial Cancer in Polish Women. Anticancer Res. 2018;38:5223-5229 pubmed publisher
    ..1 (XRCC1)-Arg399Gln (rs25487); XRCC2-Arg188His (rs3218536); XRCC3-Thr241Met (rs861539); ERCC excision repair 2, TFIIH core complex helicase subunit (ERCC2)-Lys751Gln (rs13181); and 8-oxoguanine DNA glycosylase (OGG1)-Ser326Cys (..
  58. Kim J, Saint André C, Lim H, Hwang C, Egly J, Cho Y. Crystal structure of the Rad3/XPD regulatory domain of Ssl1/p44. J Biol Chem. 2015;290:8321-30 pubmed publisher
    The Ssl1/p44 subunit is a core component of the yeast/mammalian general transcription factor TFIIH, which is involved in transcription and DNA repair. Ssl1/p44 binds to and stimulates the Rad3/XPD helicase activity of TFIIH...
  59. Devos M, Mommaerts E, Migeot V, van Bakel H, Hermand D. Fission yeast Cdk7 controls gene expression through both its CAK and C-terminal domain kinase activities. Mol Cell Biol. 2015;35:1480-90 pubmed publisher
    ..CAK) complex, and serine 5 within the polymerase II (Pol II) C-terminal domain (CTD) as transcription factor TFIIH-bound CAK. However, the physiological importance of integrating these processes is not understood...
  60. Tajedin L, Tarique M, Tuteja R. Plasmodium falciparum XPD translocates in 5' to 3' direction, is expressed throughout the blood stages, and interacts with p44. Protoplasma. 2015;252:1487-504 pubmed publisher
    XPD helicase, a TFIIH subunit, is essential for several processes including transcription, NER, cell cycle regulation, and apoptosis in eukaryotes...
  61. Clavijo R, Arora H, Gibbs E, Cohen S, Griswold A, Bakircioglu E, et al. Whole Exome Sequencing of a Consanguineous Turkish Family Identifies a Mutation in GTF2H3 in Brothers With Spermatogenic Failure. Urology. 2018;120:86-89 pubmed publisher
    ..A nonsynonymous variant in "general transcription factor TFIIH subunit 3" (GTF2H3) was identified in this consanguineous family...
  62. Stettler K, Li X, Sandrock B, Braga Lagache S, Heller M, Dümbgen L, et al. A Drosophila XPD model links cell cycle coordination with neuro-development and suggests links to cancer. Dis Model Mech. 2015;8:81-91 pubmed publisher
    ..showed how different xpd mutations affected the formation or stability of different transcription factor IIH (TFIIH) subcomplexes...
  63. Cyr N, de La Fuente C, Lecoq L, Guendel I, Chabot P, Kehn Hall K, et al. A ΩXaV motif in the Rift Valley fever virus NSs protein is essential for degrading p62, forming nuclear filaments and virulence. Proc Natl Acad Sci U S A. 2015;112:6021-6 pubmed publisher
    ..through interactions between the viral nonstructural protein NSs and the host general transcription factor TFIIH. Here, we identify an interaction between a ΩXaV motif in NSs and the p62 subunit of TFIIH...
  64. Gibbons B, Brignole E, Azubel M, Murakami K, Voss N, Bushnell D, et al. Subunit architecture of general transcription factor TFIIH. Proc Natl Acad Sci U S A. 2012;109:1949-54 pubmed publisher
    Structures of complete 10-subunit yeast TFIIH and of a nested set of subcomplexes, containing 5, 6, and 7 subunits, have been determined by electron microscopy (EM) and 3D reconstruction...
  65. Vashisht A, Yu C, Sharma T, Ro K, Wohlschlegel J. The Association of the Xeroderma Pigmentosum Group D DNA Helicase (XPD) with Transcription Factor IIH Is Regulated by the Cytosolic Iron-Sulfur Cluster Assembly Pathway. J Biol Chem. 2015;290:14218-25 pubmed publisher
    Xeroderma pigmentosum group D (XPD) helicase is a component of the transcription factor IIH (TFIIH) transcription complex and plays essential roles in transcription and nucleotide excision repair...
  66. Nag R, Niggli S, Sousa Guimarães S, Vazquez Pianzola P, Suter B. Mms19 is a mitotic gene that permits Cdk7 to be fully active as a Cdk-activating kinase. Development. 2018;145: pubmed publisher
    ..When bound to Xpd and TFIIH, the CAK subunit Cdk7 phosphorylates transcriptional targets and not cell cycle Cdks...
  67. Li C, Golebiowski F, Onishi Y, Samara N, Sugasawa K, Yang W. Tripartite DNA Lesion Recognition and Verification by XPC, TFIIH, and XPA in Nucleotide Excision Repair. Mol Cell. 2015;59:1025-34 pubmed publisher
    Transcription factor IIH (TFIIH) is essential for both transcription and nucleotide excision repair (NER)...
  68. Rodríguez Molina J, Tseng S, Simonett S, Taunton J, Ansari A. Engineered Covalent Inactivation of TFIIH-Kinase Reveals an Elongation Checkpoint and Results in Widespread mRNA Stabilization. Mol Cell. 2016;63:433-44 pubmed publisher
    During transcription initiation, the TFIIH-kinase Kin28/Cdk7 marks RNA polymerase II (Pol II) by phosphorylating the C-terminal domain (CTD) of its largest subunit...
  69. Kemp M. DNA damage-induced ATM- and Rad-3-related (ATR) kinase activation in non-replicating cells is regulated by the XPB subunit of transcription factor IIH (TFIIH). J Biol Chem. 2017;292:12424-12435 pubmed publisher
    ..Together, these results reveal a previously unknown role for transcription factor IIH in ATR kinase activation in non-replicating, non-cycling cells. ..
  70. Eyboulet F, Wydau Dematteis S, Eychenne T, Alibert O, Neil H, Boschiero C, et al. Mediator independently orchestrates multiple steps of preinitiation complex assembly in vivo. Nucleic Acids Res. 2015;43:9214-31 pubmed publisher
    ..med17 mutations differentially affected the global presence of several PIC components including Mediator, TBP, TFIIH modules and Pol II...
  71. Kuschal C, Botta E, Orioli D, DiGiovanna J, Seneca S, Keymolen K, et al. GTF2E2 Mutations Destabilize the General Transcription Factor Complex TFIIE in Individuals with DNA Repair-Proficient Trichothiodystrophy. Am J Hum Genet. 2016;98:627-42 pubmed publisher
    ..by RNA polymerase II (RNA pol II) via direct interaction with the basal transcription/DNA repair factor IIH (TFIIH)...
  72. Okuda M, Higo J, Komatsu T, Konuma T, Sugase K, Nishimura Y. Dynamics of the Extended String-Like Interaction of TFIIE with the p62 Subunit of TFIIH. Biophys J. 2016;111:950-62 pubmed publisher
    ..Upon binding to the p62 subunit of TFIIH, the acidic region adopts an extended string-like structure on the basic groove of the pleckstrin homology domain (..
  73. Coin F, Egly J. Revisiting the Function of CDK7 in Transcription by Virtue of a Recently Described TFIIH Kinase Inhibitor. Mol Cell. 2015;59:513-4 pubmed publisher
    ..2015) took advantage of THZ1, a recently described covalent inhibitor of the TFIIH kinase CDK7, to further characterize the role of this enzyme in the early stages of transcription and ..
  74. Miwa K, Kojima R, Obita T, Ohkuma Y, Tamura Y, Mizuguchi M. Crystal Structure of Human General Transcription Factor TFIIE at Atomic Resolution. J Mol Biol. 2016;428:4258-4266 pubmed publisher
    ..TFIIE subunits ? and ? form a heterodimer and recruit TFIIH to complete the assembly of the pre-initiation complex...
  75. Song X, Wang S, Hong X, Li X, Zhao X, Huai C, et al. Single nucleotide polymorphisms of nucleotide excision repair pathway are significantly associated with outcomes of platinum-based chemotherapy in lung cancer. Sci Rep. 2017;7:11785 pubmed publisher
    ..Further subgroup analysis showed that GTF2H4 presented consecutive significant signals in clinical benefit among adenocarcimoma...
  76. Narita T, Narita K, Takedachi A, Saijo M, Tanaka K. Regulation of Transcription Elongation by the XPG-TFIIH Complex Is Implicated in Cockayne Syndrome. Mol Cell Biol. 2015;35:3178-88 pubmed publisher
    ..XPG interacted with transcription elongation factors in concert with TFIIH, suggesting that the XPG-TFIIH complex serves as a transcription elongation factor...
  77. Yi J, Huan X, Song S, Zhou H, Wang Y, Miao Z. Triptolide Induces Cell Killing in Multidrug-Resistant Tumor Cells via CDK7/RPB1 Rather than XPB or p44. Mol Cancer Ther. 2016;15:1495-503 pubmed publisher
    ..In addition, XPB and p44, two transcription factor TFIIH subunits, did not contribute to triptolide-driven RPB1 degradation and cell killing, although XPB was reported to ..
  78. Ly H, Ikegami T. Rift Valley fever virus NSs protein functions and the similarity to other bunyavirus NSs proteins. Virol J. 2016;13:118 pubmed publisher
    ..cullin 1-Skp1-Fbox E3 ligase complex, the NSs protein promotes the degradation of at least two host proteins, the TFIIH p62 and the PKR proteins...
  79. Alekseev S, Nagy Z, Sandoz J, Weiss A, Egly J, Le May N, et al. Transcription without XPB Establishes a Unified Helicase-Independent Mechanism of Promoter Opening in Eukaryotic Gene Expression. Mol Cell. 2017;65:504-514.e4 pubmed publisher
    ..Current models suggest that class II gene transcription requires ATP and the TFIIH XPB subunit to open a promoter...
  80. Theil A, Hoeijmakers J, Vermeulen W. TTDA: big impact of a small protein. Exp Cell Res. 2014;329:61-8 pubmed publisher
    ..The multi-subunit transcription/repair factor IIH (TFIIH) is an important decision maker in NER, by opening the DNA double helix after the initial damage recognition and ..
  81. Lee B, Lee H, Cho H, Suh D, Kim K, No J, et al. Ataxia-Telangiectasia and RAD3-Related and Ataxia-Telangiectasia-Mutated Proteins in Epithelial Ovarian Carcinoma: Their Expression and Clinical Significance. Anticancer Res. 2015;35:3909-16 pubmed
    The expression patterns of the key DNA damage response-related proteins, ataxia-telangiectasia and tfiih/ner complex atp-dependent 5'-3' dna helicase subunit rad3 (RAD3)-related (ATR) and ataxia-telangiectasia-mutated (ATM) proteins in ..
  82. Chlamydas S, Holz H, Samata M, Chelmicki T, Georgiev P, Pelechano V, et al. Functional interplay between MSL1 and CDK7 controls RNA polymerase II Ser5 phosphorylation. Nat Struct Mol Biol. 2016;23:580-9 pubmed publisher
    ..of MSL1 with CDK7, a subunit of the Cdk-activating kinase (CAK) complex of the general transcription factor TFIIH. Importantly, MSL1 depletion leads to decreased phosphorylation of Ser5 of RNA polymerase II...
  83. Keck F, Amaya M, Kehn Hall K, Roberts B, Bailey C, Narayanan A. Characterizing the effect of Bortezomib on Rift Valley Fever Virus multiplication. Antiviral Res. 2015;120:48-56 pubmed publisher
    ..shown that the UPS plays a central role in RVFV multiplication involving degradation of PKR and p62 subunit of TFIIH. Using the FDA-approved proteasome inhibitor Bortezomib, we observed robust inhibition of intracellular and ..
  84. Deshmukh A, Mitra P, Maruthi M. Cdk7 mediates RPB1-driven mRNA synthesis in Toxoplasma gondii. Sci Rep. 2016;6:35288 pubmed publisher
    ..7 in conjunction with CyclinH and Mat1 activates cell cycle CDKs and is a part of the general transcription factor TFIIH. Role of Cdk7 is well characterized in model eukaryotes however its relevance in protozoan parasites has not been ..
  85. Han Y, Huang W, Liu J, Liu D, Cui Y, Huang R, et al. Triptolide Inhibits the AR Signaling Pathway to Suppress the Proliferation of Enzalutamide Resistant Prostate Cancer Cells. Theranostics. 2017;7:1914-1927 pubmed publisher
    ..As a result, TPL suppresses the binding of AR to promoter regions in AR target genes along with reduced TFIIH and RNA Pol II recruitment...
  86. Lee J, Mitchell N, Zaytseva O, Chahal A, Mendis P, Cartier Michaud A, et al. Defective Hfp-dependent transcriptional repression of dMYC is fundamental to tissue overgrowth in Drosophila XPB models. Nat Commun. 2015;6:7404 pubmed publisher
    ..but not others, with similar C-terminal mutations in the XPB helicase subunit of transcription and NER factor TFIIH. Mechanisms driving overproliferation and, therefore, cancer associated with XPB mutations are currently unknown...
  87. Lye M, Visuvanathan S, Chong P, Yap Y, Lim C, Ban E. Homozygous Wildtype of XPD K751Q Polymorphism Is Associated with Increased Risk of Nasopharyngeal Carcinoma in Malaysian Population. PLoS ONE. 2015;10:e0130530 pubmed publisher
    ..pigmentosum group D (XPD) gene encodes a DNA helicase, an important component in transcription factor IIH (TFIIH) complex...
  88. Compe E, Egly J. Nucleotide Excision Repair and Transcriptional Regulation: TFIIH and Beyond. Annu Rev Biochem. 2016;85:265-90 pubmed publisher
    Transcription factor IIH (TFIIH) is a multiprotein complex involved in both transcription and DNA repair, revealing a striking functional link between these two processes...
  89. Harrod A, Fulton J, Nguyen V, Periyasamy M, Ramos Garcia L, Lai C, et al. Genomic modelling of the ESR1 Y537S mutation for evaluating function and new therapeutic approaches for metastatic breast cancer. Oncogene. 2017;36:2286-2296 pubmed publisher
    ..Further, we show that the basal transcription factor TFIIH is constitutively recruited by ER-Y537S, resulting in ligand-independent phosphorylation of Serine 118 (Ser118) by ..
  90. Scully R, Anderson S, Chao D, Wei W, Ye L, Young R, et al. BRCA1 is a component of the RNA polymerase II holoenzyme. Proc Natl Acad Sci U S A. 1997;94:5605-10 pubmed
    ..also specifically purifies transcriptionally active RNA polymerase II and transcription factors TFIIF, TFIIE, and TFIIH. Moreover, a BRCA1 domain, which is deleted in about 90% of clinically relevant mutations, participates in binding ..
  91. Drapkin R, Le Roy G, Cho H, Akoulitchev S, Reinberg D. Human cyclin-dependent kinase-activating kinase exists in three distinct complexes. Proc Natl Acad Sci U S A. 1996;93:6488-93 pubmed
    Transcription factor IIH (TFIIH) is a multisubunit complex required for transcription and for DNA nucleotide excision repair...
  92. Yuan X, Zhao J, Zentgraf H, Hoffmann Rohrer U, Grummt I. Multiple interactions between RNA polymerase I, TIF-IA and TAF(I) subunits regulate preinitiation complex assembly at the ribosomal gene promoter. EMBO Rep. 2002;3:1082-7 pubmed
    ..The results uncover an interphase for essential protein-protein interactions that facilitate Pol I preinitiation complex formation. ..