GORASP1

Summary

Gene Symbol: GORASP1
Description: golgi reassembly stacking protein 1
Alias: GOLPH5, GRASP65, P65, Golgi reassembly-stacking protein 1, Golgi peripheral membrane protein p65, Golgi phosphoprotein 5, Golgi reassembly and stacking protein 1, golgi reassembly stacking protein 1, 65kDa, golgi reassembly-stacking protein of 65 kDa
Species: human
Products:     GORASP1

Top Publications

  1. Wang Y, Seemann J, Pypaert M, Shorter J, Warren G. A direct role for GRASP65 as a mitotically regulated Golgi stacking factor. EMBO J. 2003;22:3279-90 pubmed
    Cell-free assays that mimic the disassembly and reassembly cycle of the Golgi apparatus during mitosis implicated GRASP65 as a mitotically regulated stacking factor...
  2. Barr F, Puype M, Vandekerckhove J, Warren G. GRASP65, a protein involved in the stacking of Golgi cisternae. Cell. 1997;91:253-62 pubmed
    ..cisternal stacking in a cell-free system, justifying its designation as a Golgi ReAssembly Stacking Protein (GRASP65)...
  3. Barr F, Nakamura N, Warren G. Mapping the interaction between GRASP65 and GM130, components of a protein complex involved in the stacking of Golgi cisternae. EMBO J. 1998;17:3258-68 pubmed
    The nature of the complex containing GRASP65, a membrane protein involved in establishing the stacked structure of the Golgi apparatus, and GM130, a putative Golgi matrix protein and vesicle docking receptor, was investigated...
  4. Lane J, Lucocq J, Pryde J, Barr F, Woodman P, Allan V, et al. Caspase-mediated cleavage of the stacking protein GRASP65 is required for Golgi fragmentation during apoptosis. J Cell Biol. 2002;156:495-509 pubmed
    ..We have found that fragmentation is caspase dependent and identified GRASP65 (Golgi reassembly and stacking protein of 65 kD) as a novel caspase substrate...
  5. Moyer B, Allan B, Balch W. Rab1 interaction with a GM130 effector complex regulates COPII vesicle cis--Golgi tethering. Traffic. 2001;2:268-76 pubmed
    ..Here, we demonstrate that the cis-Golgi tethering protein GM130, complexed with GRASP65 and other proteins, forms a novel Rab1 effector complex that interacts with activated Rab1-GTP in a p115-..
  6. Marra P, Maffucci T, Daniele T, Tullio G, Ikehara Y, Chan E, et al. The GM130 and GRASP65 Golgi proteins cycle through and define a subdomain of the intermediate compartment. Nat Cell Biol. 2001;3:1101-13 pubmed
    ..To gain insight into this step, we investigated the dynamics by which cis-Golgi matrix proteins such as GM130 and GRASP65 associate with, and incorporate, incoming IC elements...
  7. Preisinger C, Körner R, Wind M, Lehmann W, Kopajtich R, Barr F. Plk1 docking to GRASP65 phosphorylated by Cdk1 suggests a mechanism for Golgi checkpoint signalling. EMBO J. 2005;24:753-65 pubmed
    b>GRASP65, a structural protein of the Golgi apparatus, has been linked to the sensing of Golgi structure and the integration of this information with the control of mitotic entry in the form of a Golgi checkpoint...
  8. Tang D, Yuan H, Wang Y. The role of GRASP65 in Golgi cisternal stacking and cell cycle progression. Traffic. 2010;11:827-42 pubmed publisher
    In vitro assays identified the Golgi peripheral protein GRASP65 as a Golgi stacking factor that links adjacent Golgi cisternae by forming mitotically regulated trans-oligomers...
  9. Sutterlin C, Lin C, Feng Y, Ferris D, Erikson R, Malhotra V. Polo-like kinase is required for the fragmentation of pericentriolar Golgi stacks during mitosis. Proc Natl Acad Sci U S A. 2001;98:9128-32 pubmed
    ..GM130 and GRASP65 (Golgi reassembly stacking protein of 65 kDa) are Golgi-associated proteins that are targets of mitotic kinases, and they have also been ..

More Information

Publications109 found, 100 shown here

  1. Yoshimura S, Yoshioka K, Barr F, Lowe M, Nakayama K, Ohkuma S, et al. Convergence of cell cycle regulation and growth factor signals on GRASP65. J Biol Chem. 2005;280:23048-56 pubmed
    Together with other Golgi matrix components, GRASP65 contributes to the stacking of Golgi cisternae in interphase cells...
  2. D Angelo G, Prencipe L, Iodice L, Beznoussenko G, Savarese M, Marra P, et al. GRASP65 and GRASP55 sequentially promote the transport of C-terminal valine-bearing cargos to and through the Golgi complex. J Biol Chem. 2009;284:34849-60 pubmed publisher
    The Golgi matrix proteins GRASP65 and GRASP55 have recognized roles in maintaining the architecture of the Golgi complex, in mitotic progression and in unconventional protein secretion whereas, surprisingly, they have been shown to be ..
  3. Shorter J, Watson R, Giannakou M, Clarke M, Warren G, Barr F. GRASP55, a second mammalian GRASP protein involved in the stacking of Golgi cisternae in a cell-free system. EMBO J. 1999;18:4949-60 pubmed
    ..GRASP55 is homologous to GRASP65, an N-ethylmaleimide-sensitive membrane protein required for the stacking of Golgi cisternae in a cell-free ..
  4. Lin C, Madsen M, Yarm F, Jang Y, Liu X, Erikson R. Peripheral Golgi protein GRASP65 is a target of mitotic polo-like kinase (Plk) and Cdc2. Proc Natl Acad Sci U S A. 2000;97:12589-94 pubmed
    ..mammalian Plk C-terminal domain baits, we have identified Golgi peripheral protein GRASP65 (Golgi reassembly stacking protein of 65 kDa) as a Plk-binding protein...
  5. Barr F, Preisinger C, Kopajtich R, Korner R. Golgi matrix proteins interact with p24 cargo receptors and aid their efficient retention in the Golgi apparatus. J Cell Biol. 2001;155:885-91 pubmed
    ..from a family of coiled-coil proteins, the golgins, and other peripheral membrane components such as GRASP55 and GRASP65. Here we find that TMP21, p24a, and gp25L, members of the p24 cargo receptor family, are present in complexes ..
  6. Puthenveedu M, Bachert C, Puri S, Lanni F, Linstedt A. GM130 and GRASP65-dependent lateral cisternal fusion allows uniform Golgi-enzyme distribution. Nat Cell Biol. 2006;8:238-48 pubmed
    ..by specific membrane-fusion events that occur during Golgi assembly, and require the Golgi proteins GM130 and GRASP65. Furthermore, these GM130 and GRASP65-dependent lateral cisternal-fusion reactions are necessary to achieve ..
  7. Xiang Y, Wang Y. GRASP55 and GRASP65 play complementary and essential roles in Golgi cisternal stacking. J Cell Biol. 2010;188:237-51 pubmed publisher
    In vitro studies have suggested that Golgi stack formation involves two homologous peripheral Golgi proteins, GRASP65 and GRASP55, which localize to the cis and medial-trans cisternae, respectively...
  8. Sengupta D, Linstedt A. Mitotic inhibition of GRASP65 organelle tethering involves Polo-like kinase 1 (PLK1) phosphorylation proximate to an internal PDZ ligand. J Biol Chem. 2010;285:39994-40003 pubmed publisher
    b>GRASP65 links cis-Golgi cisternae via a homotypic, N-terminal PDZ interaction, and its mitotic phosphorylation disrupts this activity...
  9. Ward T, Polishchuk R, Caplan S, Hirschberg K, Lippincott Schwartz J. Maintenance of Golgi structure and function depends on the integrity of ER export. J Cell Biol. 2001;155:557-70 pubmed
    ..Instead, they suggest that the Golgi complex is a dynamic, steady-state system, whose membranes can be nucleated and are maintained by the activities of the Sar1-COPII and Arf1-coatomer systems...
  10. Short B, Preisinger C, Korner R, Kopajtich R, Byron O, Barr F. A GRASP55-rab2 effector complex linking Golgi structure to membrane traffic. J Cell Biol. 2001;155:877-83 pubmed
    ..with the vesicle tethering factor p115 and the cis-Golgi matrix protein GM130, also part of a complex with GRASP65 important for the organization of cis-Golgi cisternae...
  11. Kodani A, Sutterlin C. The Golgi protein GM130 regulates centrosome morphology and function. Mol Biol Cell. 2008;19:745-53 pubmed
    ..Although GA proteins have been described to regulate mitotic centrosomes and spindle formation, this is the first report of a role for a GA protein in the regulation of centrosomes during interphase. ..
  12. Sengupta D, Truschel S, Bachert C, Linstedt A. Organelle tethering by a homotypic PDZ interaction underlies formation of the Golgi membrane network. J Cell Biol. 2009;186:41-55 pubmed publisher
    Formation of the ribbon-like membrane network of the Golgi apparatus depends on GM130 and GRASP65, but the mechanism is unknown...
  13. Wang Y, Satoh A, Warren G. Mapping the functional domains of the Golgi stacking factor GRASP65. J Biol Chem. 2005;280:4921-8 pubmed
    ..b>GRASP65 is a dimer that can directly link adjacent surfaces through trans-oligomerization in a mitotically regulated ..
  14. Sutterlin C, Polishchuk R, Pecot M, Malhotra V. The Golgi-associated protein GRASP65 regulates spindle dynamics and is essential for cell division. Mol Biol Cell. 2005;16:3211-22 pubmed
    ..The Golgi-associated protein GRASP65 is involved in this process...
  15. Xiang Y, Zhang X, Nix D, Katoh T, Aoki K, Tiemeyer M, et al. Regulation of protein glycosylation and sorting by the Golgi matrix proteins GRASP55/65. Nat Commun. 2013;4:1659 pubmed publisher
    ..b>GRASP65 and GRASP55, two proteins localized to the Golgi stack and early secretory pathway, mediate processes including ..
  16. Xu H, Jiang W, Feng L, Liu Y, Wu P, Jiang J, et al. Dietary vitamin C deficiency depresses the growth, head kidney and spleen immunity and structural integrity by regulating NF-κB, TOR, Nrf2, apoptosis and MLCK signaling in young grass carp (Ctenopharyngodon idella). Fish Shellfish Immunol. 2016;52:111-38 pubmed publisher
    ..interferon γ2, IL-1β, IL-6, IL-8, IL-12 P35 (only in spleen), IL-12 P40, IL-15, IL-17D, nuclear factor κB p65, IκB kinases (IKKα, IKKβ, IKKγ), target of rapamycin and ribosomal protein S6 kinase 1 mRNA levels (P &..
  17. Xu H, Jiang W, Feng L, Liu Y, Wu P, Jiang J, et al. Dietary vitamin C deficiency depressed the gill physical barriers and immune barriers referring to Nrf2, apoptosis, MLCK, NF-?B and TOR signaling in grass carp (Ctenopharyngodon idella) under infection of Flavobacterium columnare. Fish Shellfish Immunol. 2016;58:177-192 pubmed publisher
    ..cytokines-related factors interferon ?2, IL-1?, IL-6, IL-8, IL-12 P35, IL-12 P40, nuclear factor ?B (NF-?B) p65 (rather than NF-?B p52), I?B kinases (IKK) (only IKK? and IKK?), target of rapamycin and ribosomal protein S6 ..
  18. Cui X, Qian D, Jiang S, Shang E, Zhu Z, Duan J. Scutellariae Radix and Coptidis Rhizoma Improve Glucose and Lipid Metabolism in T2DM Rats via Regulation of the Metabolic Profiling and MAPK/PI3K/Akt Signaling Pathway. Int J Mol Sci. 2018;19: pubmed publisher
    ..Protein expressions of p65; p-p65; phosphatidylinositol-4,5-bisphosphate 3-kinase (PI3K); phosphorylated-PI3K (p-PI3K); protein kinase B (Akt);..
  19. Simon Y, Kessler S, Gemperlein K, Bohle R, Müller R, Haybaeck J, et al. Elevated free cholesterol in a p62 overexpression model of non-alcoholic steatohepatitis. World J Gastroenterol. 2014;20:17839-50 pubmed publisher
    ..Additionally, nuclear localization of the nuclear factor kappa B (NF-κB) subunit p65 was examined in frozen tissues...
  20. Li S, Feng L, Jiang W, Liu Y, Jiang J, Wu P, et al. Deficiency of dietary niacin impaired gill immunity and antioxidant capacity, and changes its tight junction proteins via regulating NF-?B, TOR, Nrf2 and MLCK signaling pathways in young grass carp (Ctenopharyngodon idella). Fish Shellfish Immunol. 2016;55:212-22 pubmed publisher
    ..necrosis factor ?, interleukin 8, interferon ?2, and interleukin 1?), signaling molecules (nuclear factor kappa B p65, I?B kinase ?, I?B kinase ?, I?B kinase ?, Kelch-like-ECH-associated protein 1b, myosin light chain kinase and p38 ..
  21. Li J, Shi J, Li P, Guo X, Wang T, Liu A. Genipin attenuates hyperoxia-induced lung injury and pulmonary hypertension via targeting glycogen synthase kinase-3 β in neonatal rats. Nutrition. 2019;57:237-244 pubmed publisher
    ..In addition, genipin inhibited p65 nuclear factor-κB nuclear translocation and matrix metalloproteinase-2 and -9 expression...
  22. Tian Y, Xu J, Li Y, Zhao R, Du S, Lv C, et al. MicroRNA-31 Reduces Inflammatory Signaling and Promotes Regeneration in Colon Epithelium, and Delivery of Mimics in Microspheres Reduces Colitis in Mice. Gastroenterology. 2019;: pubmed publisher
    ..immunoprecipitation and luciferase assays to study proteins that regulate expression of MIR31, including STAT3 and p65, in LOVO colorectal cancer cells and organoids derived from mouse colon cells...
  23. Liu S, Feng L, Jiang W, Liu Y, Jiang J, Wu P, et al. Impact of exogenous lipase supplementation on growth, intestinal function, mucosal immune and physical barrier, and related signaling molecules mRNA expression of young grass carp (Ctenopharyngodon idella). Fish Shellfish Immunol. 2016;55:88-105 pubmed publisher
    ..factor ?, interleukin 8, interferon ?2, and interleukin 1?), and signaling molecules (nuclear factor kappa B p65, I?B kinase ?, I?B kinase ?) (P < 0.05) in the intestine of young grass carp...
  24. Bai R, Yin X, Feng X, Cao Y, Wu Y, Zhu Z, et al. Corydalis hendersonii Hemsl. protects against myocardial injury by attenuating inflammation and fibrosis via NF-?B and JAK2-STAT3 signaling pathways. J Ethnopharmacol. 2017;207:174-183 pubmed publisher
    ..MMP-2) and MMP-9 in the cardiac tissue homogenate, protein expressions of signal-transduction proteins, p65, I?B?, JAK2, and STAT3 in heart tissues were measured by ELISA and Western blot analyses...
  25. Pan F, Feng L, Jiang W, Jiang J, Wu P, Kuang S, et al. Methionine hydroxy analogue enhanced fish immunity via modulation of NF-?B, TOR, MLCK, MAPKs and Nrf2 signaling in young grass carp (Ctenopharyngodon idella). Fish Shellfish Immunol. 2016;56:208-228 pubmed publisher
    ..anti-inflammatory cytokines mRNA levels, which might be attributed to the down-regulation of nuclear factor ?B p65, c-Rel, I?B kinase ?, p38 mitogen activated protein kinase, eIF4E-binding protein1 (4E-BP1) and 4E-BP2 mRNA levels ..
  26. Guo Y, Jiang W, Wu P, Liu Y, Zhou X, Kuang S, et al. The decreased growth performance and impaired immune function and structural integrity by dietary iron deficiency or excess are associated with TOR, NF-?B, p38MAPK, Nrf2 and MLCK signaling in head kidney, spleen and skin of grass carp (Ctenopharyngod. Fish Shellfish Immunol. 2017;65:145-168 pubmed publisher
    ..kinase 1 (S6K1), whereas up-regulated the mRNA levels of pro-inflammatory cytokines, nuclear factor kappa B (NF-?B) p65, I?B kinases ? (IKK?) and eIF4E-binding protein (4E-BP) in head kidney and spleen of young grass carp (P < 0...
  27. Shedlock D, Tingey C, Mahadevan L, Hutnick N, Reuschel E, Kudchodkar S, et al. Co-Administration of Molecular Adjuvants Expressing NF-Kappa B Subunit p65/RelA or Type-1 Transactivator T-bet Enhance Antigen Specific DNA Vaccine-Induced Immunity. Vaccines (Basel). 2014;2:196-215 pubmed publisher
    ..This study demonstrates the use of cellular transcription factors as adjuvants for enhancing DNA vaccine-induced immunity. ..
  28. Tong F, Zhang J, Liu L, Gao X, Cai Q, Wei C, et al. Corilagin Attenuates Radiation-Induced Brain Injury in Mice. Mol Neurobiol. 2016;53:6982-6996 pubmed
    ..signal transducers and activators of transcription 3 (p-STAT3) and IκBα, and the translocation of p65 from cytoplasm to nucleus were detected by using Western blotting...
  29. Ren H, Wang Z, Zhang S, Ma H, Wang Y, Jia L, et al. IL-17A Promotes the Migration and Invasiveness of Colorectal Cancer Cells Through NF-κB-Mediated MMP Expression. Oncol Res. 2016;23:249-56 pubmed publisher
    ..Moreover, the nuclear/overall fractions and DNA-binding activity of p65 and p50 were dramatically elevated by IL-17A...
  30. Li Z, Wang X, Pan H, Yang H, Li X, Zhang K, et al. Resistin promotes CCL4 expression through toll-like receptor-4 and activation of the p38-MAPK and NF-κB signaling pathways: implications for intervertebral disc degeneration. Osteoarthritis Cartilage. 2017;25:341-350 pubmed publisher
    ..A ChIP assay and lentivirus mediated knockdown showed that resistin regulate CCL4 expression through p65. This study shows that resistin binds to TLR4 and increase the expression of CCL4 through p38-MAPK and NF-κB ..
  31. Chen R, Wang H, Liang B, Liu G, Tang M, Jia R, et al. Downregulation of ASPP2 improves hepatocellular carcinoma cells survival via promoting BECN1-dependent autophagy initiation. Cell Death Dis. 2016;7:e2512 pubmed publisher
    ..Firstly, ASPP2 inhibits p65/RelA-induced transcription of BECN1, directly by an ASPP2-p65/RelA-IκBα complex which inhibits ..
  32. Gundala N, Naidu V, Das U. Arachidonic acid and lipoxinA4 attenuate streptozotocin-induced cytotoxicity to RIN5 F cells in vitro and type 1 and type 2 diabetes mellitus in vivo. Nutrition. 2017;35:61-80 pubmed publisher
    ..Changes in the antioxidant content, lipid peroxides, nitric oxide, and expression of PDX1, P65, nuclear factor-?b (NF-?b), and IKB genes in STZ-treated RIN5 F cells in vitro and Nrf2, GLUT2, COX2, iNOS ..
  33. Chen L, Lan Z. Polydatin attenuates potassium oxonate-induced hyperuricemia and kidney inflammation by inhibiting NF-?B/NLRP3 inflammasome activation via the AMPK/SIRT1 pathway. Food Funct. 2017;8:1785-1792 pubmed publisher
    ..Western blot analyses illustrated that polydatin down-regulated the translocation of NF-?B p65, the degradation of I?B?, and the protein levels of inflammasome components (NLRP3, ASC, and caspase-1), which led ..
  34. Chen L, Lai Y, Dong L, Kang S, Chen X. Polysaccharides from Citrus grandis L. Osbeck suppress inflammation and relieve chronic pharyngitis. Microb Pathog. 2017;113:365-371 pubmed publisher
    ..Protein amount of IKK? and p65, IKK?/? activity and p65 activity were evaluated by Western blot and luciferase assay...
  35. Wang K, Wu J, Cheng F, Huang X, Zeng F, Zhang Y. Acidic Polysaccharide from Angelica sinensis Reverses Anemia of Chronic Disease Involving the Suppression of Inflammatory Hepcidin and NF-?B Activation. Oxid Med Cell Longev. 2017;2017:7601592 pubmed publisher
    ..Furthermore, ASP inhibited NF-?B p65 activation via the I?B kinases- (IKKs-) I?B? pathway, thereby reducing the secretion of ..
  36. Fu S, Li Y, Wu Y, Yue Y, Qian Z, Yang D. Icariside II attenuates myocardial fibrosis by inhibiting nuclear factor-?B and the TGF-?1/Smad2 signalling pathway in spontaneously hypertensive rats. Biomed Pharmacother. 2018;100:64-71 pubmed publisher
    ..factor-? (TNF-?), transforming growth factor-?1 (TGF-?1), Smad2, inhibitory ?B (I?B), and nuclear factor ?B (NF-?B) p65 by Western blot...
  37. Qin W, Xie W, Xia N, He Q, Sun T. Silencing of Transient Receptor Potential Channel 4 Alleviates oxLDL-induced Angiogenesis in Human Coronary Artery Endothelial Cells by Inhibition of VEGF and NF-?B. Med Sci Monit. 2016;22:930-6 pubmed
    ..Expression of vascular endothelial growth factor (VEGF) and nuclear factor (NF)-?B p65 were assessed by Western blotting...
  38. Singh A, Awasthi D, Dubey M, Nagarkoti S, Kumar A, Chandra T, et al. High oxidative stress adversely affects NFκB mediated induction of inducible nitric oxide synthase in human neutrophils: Implications in chronic myeloid leukemia. Nitric Oxide. 2016;58:28-41 pubmed publisher
    ..Mechanistically, we found that under high oxidative stress condition, S-glutathionylation of NFκB (p50 and p65 subunits) severely limits iNOS expression due to its reduced binding to iNOS promoter, which was reversed in ..
  39. Kong X, Qian X, Duan L, Liu H, Zhu Y, Qi J. microRNA-372 Suppresses Migration and Invasion by Targeting p65 in Human Prostate Cancer Cells. DNA Cell Biol. 2016;35:828-835 pubmed
    ..Subsequently, p65 is confirmed as a target of miR-372, and knockdown of p65 expression similarly resulted in decreased proliferation ..
  40. Cui X, Chen Q, Dong Z, Xu L, Lu T, Li D, et al. Inactivation of Sirt1 in mouse livers protects against endotoxemic liver injury by acetylating and activating NF-κB. Cell Death Dis. 2016;7:e2403 pubmed publisher
    ..against GalN/LPS-induced liver injury, which is a result of enhanced NF-κB response because knockdown of RelA/p65 negates the protective effect of Sirt1 knockout...
  41. Mani A, Vasanthi C, Gopal V, Chellathai D. Role of phyto-stabilised silver nanoparticles in suppressing adjuvant induced arthritis in rats. Int Immunopharmacol. 2016;41:17-23 pubmed publisher
    ..Furthermore, the phytostabilised silver nanoparticles (S-AgNPs) inhibited the protein expression of NF-k? p65 and TNF-? as evidenced by immunohistochemistry analysis...
  42. Liu J, Chen Q, Jian Z, Xiong X, Shao L, Jin T, et al. Daphnetin Protects against Cerebral Ischemia/Reperfusion Injury in Mice via Inhibition of TLR4/NF-?B Signaling Pathway. Biomed Res Int. 2016;2016:2816056 pubmed publisher
    ..infarct sizes, the levels of inflammatory cytokines, apoptotic neural cells, and the levels of TLR4, NF-?B p65, and I?B? were estimated...
  43. Hotter D, Krabbe T, Reith E, Gawanbacht A, Rahm N, Ayouba A, et al. Primate lentiviruses use at least three alternative strategies to suppress NF-?B-mediated immune activation. PLoS Pathog. 2017;13:e1006598 pubmed publisher
    ..complex or a factor further downstream in the NF-?B signaling cascade, only SIVolc Vpr stabilizes I?B? and inhibits p65 phosphorylation...
  44. Wang B, Xie J, He H, Huang E, Cao Q, Luo L, et al. Suppression of CLC-3 chloride channel reduces the aggressiveness of glioma through inhibiting nuclear factor-?B pathway. Oncotarget. 2017;8:63788-63798 pubmed publisher
    ..adenovirus significantly decreases volume-regulated chloride currents, inhibits the nuclear translocation of p65 subunit of Nuclear Factor-?B (NF-?B), decreases transcriptional activity of NF-?B, reduces MMP-3 and MMP-9 ..
  45. Lomert E, Turoverova L, Kriger D, Aksenov N, Nikotina A, Petukhov A, et al. Co-expression of RelA/p65 and ACTN4 induces apoptosis in non-small lung carcinoma cells. Cell Cycle. 2017;:1-21 pubmed publisher
    ..Our previous studies have demonstrated the interaction of ACTN4 with the RelA/p65 subunit of NF-kappaB factor and the effect on its transcriptional activity in A431 and HEK293T cells...
  46. Xu B, Zhang A, Li F, Cui M, Han J, Cao Q. XZ-1 regulates cell apoptosis of gastric epithelial dysplasia via NF-κB/p53/Ki67 signaling pathway. Biosci Rep. 2018;38: pubmed publisher
    ..Interestingly, XZ-1 enhanced protein expression of NF-κB p65, Ki67, and p53...
  47. Yuan B, Tang W, Lu L, Zhou Y, Zhu H, Zhou Y, et al. TLR4 upregulates CBS expression through NF-κB activation in a rat model of irritable bowel syndrome with chronic visceral hypersensitivity. World J Gastroenterol. 2015;21:8615-28 pubmed publisher
    ..7 ± 0.1 vs 1.1 ± 0.04, P < 0.05) and of the NF-κB subunit p65 (0.8 ± 0.1 vs 0.5 ± 0.1, P < 0.05)...
  48. Pan X, Meng R, Yu Z, Mou J, Liu S, Sun Z, et al. Quinalizarin enhances radiosensitivity of nasopharyngeal carcinoma cells partially by suppressing SHP-1 expression. Int J Oncol. 2016;48:1073-84 pubmed publisher
    ..Quinalizarin inhibited binding of p65 and the promoter of SHP-1, and decreased the activities of SHP-1 promoter and SHP-1...
  49. Tomita H, Tabata K, Takahashi M, Nishiyama F, Sugano E. Light induces translocation of NF-?B p65 to the mitochondria and suppresses expression of cytochrome c oxidase subunit III (COX III) in the rat retina. Biochem Biophys Res Commun. 2016;473:1013-1018 pubmed publisher
    ..Here, we report that in dark-adapted rats exposed for 6 h to bright white light, the p65 subunit of retinal NF-?B translocates to the mitochondria, an event associated with a decrease in expression of ..
  50. Pratap U, Hima L, Priyanka H, ThyagaRajan S. Noni (Morinda citrifolia L.) fruit juice reverses age-related decline in neural-immune interactions in the spleens of old F344 rats. J Ethnopharmacol. 2017;198:363-371 pubmed publisher
    ..production, expression of tyrosine hydroxylase (p-TH), nerve growth factor (NGF), m-TOR, I?B-?, p-NF-?B (p50 and p65), p-ERK, p-Akt, p-CREB and lipid peroxidation, protein carbonyl formation, nitric oxide (NO) production were ..
  51. Han E, Kim H, Lee N, Kim N, Yoo S, Kwon H, et al. Suppression of NFAT5-mediated Inflammation and Chronic Arthritis by Novel ?B-binding Inhibitors. EBioMedicine. 2017;18:261-273 pubmed publisher
    ..inhibited NFAT5 upregulation in macrophages stimulated with lipopolysaccharide and repressed the formation of NF-?B p65-DNA complexes in the NFAT5 promoter region...
  52. Faustmann G, Tiran B, Maimari T, Kieslinger P, Obermayer Pietsch B, Gruber H, et al. Circulating leptin and NF-?B activation in peripheral blood mononuclear cells across the menstrual cycle. Biofactors. 2016;42:376-87 pubmed publisher
    ..In 28 women, leptin, adiponectin, ?-carotene, and progesterone concentrations, NF-?B p65 and p50 activation in peripheral blood mononuclear cells (known to possess estrogen, progesterone and leptin ..
  53. Karuppagounder V, Arumugam S, Thandavarayan R, Sreedhar R, Giridharan V, Afrin R, et al. Curcumin alleviates renal dysfunction and suppresses inflammation by shifting from M1 to M2 macrophage polarization in daunorubicin induced nephrotoxicity in rats. Cytokine. 2016;84:1-9 pubmed publisher
    ..Renal protein expression of extracellular signal-regulated kinase (ERK)1/2 and nuclear factor (NF)?B p65 were increased in DNR treated rats, and treatment with curcumin attenuated these increased expression...
  54. Xiong Y, Chen H, Liu L, Lu L, Wang Z, Tian F, et al. microRNA-130a Promotes Human Keratinocyte Viability and Migration and Inhibits Apoptosis Through Direct Regulation of STK40-Mediated NF-?B Pathway and Indirect Regulation of SOX9-Meditated JNK/MAPK Pathway: A Potential Role in Psoriasis. DNA Cell Biol. 2017;36:219-226 pubmed publisher
    ..Overexpressing miR-130a significantly upregulated NF-?B p65, SOX9, p-c-Jun, p-JNK, and p-p38MAPK proteins and silencing miR-130a downregulated them...
  55. Varadi J, Harazin A, Fenyvesi F, Réti Nagy K, Gogolak P, Vámosi G, et al. Alpha-Melanocyte Stimulating Hormone Protects against Cytokine-Induced Barrier Damage in Caco-2 Intestinal Epithelial Monolayers. PLoS ONE. 2017;12:e0170537 pubmed publisher
    ..Further immunohistochemistry analysis revealed proinflammatory cytokine induced translocation of the NF-?B p65 subunit into Caco-2 cell nuclei, which was inhibited by ?-MSH...
  56. Chao G, Zhang S. Aquaporins 1, 3 and 8 expression in irritable bowel syndrome rats' colon via NF-κB pathway. Oncotarget. 2017;8:47175-47183 pubmed publisher
    ..The expression of AQP1, AQP3, and AQP8 of IBS model group was down-regulated while NF-κB p65 was up-regulated comparing with control group (p < 0...
  57. Palomino Alonso M, Lachén Montes M, González Morales A, Ausín K, Pérez Mediavilla A, Fernández Irigoyen J, et al. Network-Driven Proteogenomics Unveils an Aging-Related Imbalance in the Olfactory I?B?-NF?B p65 Complex Functionality in Tg2576 Alzheimer's Disease Mouse Model. Int J Mol Sci. 2017;18: pubmed publisher
    ..element binding protein 1 (CREB1) and APP interactomes, an imbalance in the functionality of the I?B?-NF?B p65 complex was observed during the aging process in the OB of Tg2576 mice...
  58. Long F, Shi M, Zhou H, Liu D, Sang N, Du J. Klotho upregulation contributes to the neuroprotection of ligustilide against cerebral ischemic injury in mice. Eur J Pharmacol. 2018;820:198-205 pubmed publisher
    ..The protective effects were associated with inhibition of the RIG-I/NF-?B p65 and Akt/FoxO1 pathways and with prevention of inflammation and oxidative stress in the brain...
  59. Li J, Lei H, Cao L, Mi Y, Li S, Cao Y. Crocin alleviates coronary atherosclerosis via inhibiting lipid synthesis and inducing M2 macrophage polarization. Int Immunopharmacol. 2018;55:120-127 pubmed publisher
    ..Finally, Crocin strongly inhibited the expression of NF-?B p65 and its translocation into the nucleus...
  60. Li Y, Lai Y, Wang Y, Liu N, Zhang F, Xu P. 1, 8-Cineol Protect Against Influenza-Virus-Induced Pneumonia in Mice. Inflammation. 2016;39:1582-93 pubmed publisher
    ..The results also showed that 1,8-cineol reduced the expression of NF-kB p65, intercellular adhesion molecule (ICAM)-1, and vascular cell adhesion molecule (VCAM)-1 in lung tissues...
  61. Lu B, Ren Y, Sun X, Han C, Wang H, Chen Y, et al. Induction of INKIT by Viral Infection Negatively Regulates Antiviral Responses through Inhibiting Phosphorylation of p65 and IRF3. Cell Host Microbe. 2017;22:86-98.e4 pubmed publisher
    The transcription factors p65 and IRF3 play key roles in the induction of cellular antiviral responses. Phosphorylation of p65 and IRF3 is required for their activity and constitutes a key checkpoint...
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    ..tumor necrosis factor ? (TNF-?), interleukin 1? (IL-1?), interleukin 10 (IL-10), nuclear factor kappa B (NF-?B) p65 protein, TNF receptor-associated factor 2 (TRAF2) and nuclear NF-?B (nNF-?B) proteins were estimated by enzyme-..
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    ..Our results suggest that the anti-inflammatory and anti-apoptotic effects of baicalein are mediated through the inhibition of the translocation of phosphorylated p65 to the nucleus.
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    ..representative transcriptional factors involved in MDR1 gene regulation, an in silico analysis revealed that NF-?B/p65 has a specific binding site for miR-138...
  67. Machova Urdzikova L, Ruzicka J, Karova K, Kloudova A, Svobodová B, Amin A, et al. A green tea polyphenol epigallocatechin-3-gallate enhances neuroregeneration after spinal cord injury by altering levels of inflammatory cytokines. Neuropharmacology. 2017;126:213-223 pubmed publisher
    ..Treatment with EGCG was effective in decreasing the nuclear translocation of subunit p65 (RelA) of the NF-?B dimer, and therefore canonical NF-?B pathway attenuation...
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  69. Zhou J, Tan L, Xie J, Lai Z, Huang Y, Qu C, et al. Characterization of brusatol self-microemulsifying drug delivery system and its therapeutic effect against dextran sodium sulfate-induced ulcerative colitis in mice. Drug Deliv. 2017;24:1667-1679 pubmed publisher
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    ..Additionally, long-term exposure of MC-LR at low concentration remarkably promoted the expression of NF-κB p65, COX-2, iNOS, TNF-α, IL-1β, and IL-6 in the cells, suggesting that long-term MC-LR exposure at low ..
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    ..Moreover, edaravone decreased the levels of NF-?B (P65) and proinflammatory cytokines TNF-?, IL-1? and IL-6 and the inflammatory proteins expression levels, HMGB1, nNOS, ..
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    ..To correlate the expression of cell adhesion molecules and NF?B (p65) in leprosy lesions, 30 skin biopsies of patients with leprosy [16 with the tuberculoid (TT) or borderline ..
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    ..reduced iNOS and COX-2 protein levels in hippocampus; xanthoceraside also inhibited translocation of NF-?B p50 and p65 into the nucleus and phosphorylation of extracellular signal-regulated kinase (ERK), Jun N-terminal kinase (JNK) ..
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    ..we found that the pro-inflammatory cytokine TNF-? and the transcription factor nuclear factor kappa B (NF-?B) p65 subunit were both upregulated in bleomycin-induced fibrotic lung tissue...
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    ..TT regulated the ROS, and decreased mRNA expression of Akt1, JAK2, PI3K?, Erk2, FAK, and NF-?B p65 and protein expression of Erk2, FAK, and NF-?B p65...
  78. Cao Q, Karthikeyan A, Dheen S, Kaur C, Ling E. Production of proinflammatory mediators in activated microglia is synergistically regulated by Notch-1, glycogen synthase kinase (GSK-3?) and NF-?B/p65 signalling. PLoS ONE. 2017;12:e0186764 pubmed publisher
    ..We have reported that Notch-1 and NF-?B/p65 signalling pathways operate in synergy in regulating the production of proinflammatory mediators in activated ..
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    ..The protein expression levels of phosphorylated p38, p-p65 and the nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor alpha (IκBα) in the ..
  80. Mateu Jimenez M, Fermoselle C, Rojo F, Mateu J, Peña R, Urtreger A, et al. Pharmacological Approaches in an Experimental Model of Non-Small Cell Lung Cancer: Effects on Tumor Biology. Curr Pharm Des. 2016;22:5300-5310 pubmed
    ..Body and tumor weights, oxidative stress, antioxidants, inflammation, NF-?B p65 expression, fibulins, apoptosis, autophagy, tumor and stroma histology were evaluated in the subcutaneous tumor of ..
  81. Jing S, Gao X, Yu B, Qiao H. Raf Kinase Inhibitor Protein (RKIP) Inhibits Tumor Necrosis Factor-? (TNF-?) Induced Adhesion Molecules Expression in Vascular Smooth Muscle Bells by Suppressing (Nuclear Transcription Factor-?B (NF-kappaB) Pathway. Med Sci Monit. 2017;23:4789-4797 pubmed
    ..The protein expression of RKIP, p65, and inhibitor of nuclear factor (NF)-?B? (I?B?) were detected by western blot analysis...
  82. Luo D, Guo Y, Cheng Y, Zhao J, Wang Y, Rong J. Natural product celastrol suppressed macrophage M1 polarization against inflammation in diet-induced obese mice via regulating Nrf2/HO-1, MAP kinase and NF-?B pathways. Aging (Albany NY). 2017;9:2069-2082 pubmed publisher
    ..ERK1/2, p38, JNK) in a concentration dependent manner; 2) attenuating LPS-induced nuclear translocation of NF-?B p65 subunit in a time dependent manner; 3) activating Nrf2 and subsequently inducing HO-1 expression...
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    ..investigations have led to the identification of megakaryocytic leukemia 1 (MKL1) as a key co-factor of NF-?B/p65 participating in TNF-? induced pro-inflammatory transcription in macrophages...
  84. Wei X, Nie S, Liu H, Sun J, Liu J, Li J, et al. Angiopoietin-like protein 2 facilitates non-small cell lung cancer progression by promoting the polarization of M2 tumor-associated macrophages. Am J Cancer Res. 2017;7:2220-2233 pubmed
    ..and recombinant protein stimulation revealed that Angptl2 fostered the M2 polarization of TAMs through the p65 nuclear factor-kappa B (NF-?B) pathway...
  85. Tsai M, Hsu L, Lee C, Chiang Y, Lee M, How J, et al. Resveratrol inhibits urban particulate matter-induced COX-2/PGE2 release in human fibroblast-like synoviocytes via the inhibition of activation of NADPH oxidase/ROS/NF-?B. Int J Biochem Cell Biol. 2017;88:113-123 pubmed publisher
    ..Finally, we demonstrated that PM enhanced NF-?B p65 phosphorylation and the NF-?B promoter activity, which were reduced by pretreatment with a ROS inhibitor or ..
  86. Shu Y, Liu X, Ma X, Gao J, He W, Cao X, et al. Immune response mechanism of mouse monocytes/macrophages treated with ?-carrageenan polysaccharide. Environ Toxicol Pharmacol. 2017;53:191-198 pubmed publisher
    ..7 cells treated with ?-carrageenan polysaccharide show upregulated TLR4 expression, and the main subunit of NF-?B (p65) is translocated. These results support the immunomodulatory function of ?-carrageenan polysaccharide in RAW 264...
  87. Guzmán Mejía F, López Rubalcava C, Gonzalez Espinosa C. Stimulation of nAchR?7 Receptor Inhibits TNF Synthesis and Secretion in Response to LPS Treatment of Mast Cells by Targeting ERK1/2 and TACE Activation. J Neuroimmune Pharmacol. 2018;13:39-52 pubmed publisher
    ..was found that GTS-21 also diminished secretion of de novo synthesized TNF, TNF mRNA accumulation and IKK-dependent p65-NF?B phosphorylation in response to LPS...
  88. Park Y, Sultan M, Park H, Lee J, Ju H, Lee O, et al. NF-?B signaling is key in the wound healing processes of silk fibroin. Acta Biomater. 2018;67:183-195 pubmed publisher
    ..confirmed the activation of the NF-?B signaling pathway as SF induced protein expression levels of IKK?, IKK?, p65, and the degradation of I?B?...
  89. Sweeney E, Kallapur S, Meawad S, Gisslen T, Stephenson S, Jobe A, et al. Ureaplasma Species Multiple Banded Antigen (MBA) Variation Is Associated with the Severity of Inflammation In vivo and In vitro in Human Placentae. Front Cell Infect Microbiol. 2017;7:123 pubmed publisher
    ..i>In vitro, recombinant-MBA variants elicited different cytokine responses and altered expression of NF-?B p65. Conclusion: This study demonstrates that size variation of the ureaplasma MBA protein modulates the host ..
  90. Li X, Wang L, Chen C. Effects of exogenous thymosin β4 on carbon tetrachloride-induced liver injury and fibrosis. Sci Rep. 2017;7:5872 pubmed publisher
    ..Furthermore, up-regulations of nuclear factor-κB p65 protein expression by CCl4 treatment in mice and rats livers were also remarkably reduced by exogenous ..
  91. Li Y, Kong F, Shao Q, Wang R, Hu E, Liu J, et al. YAP Expression and Activity Are Suppressed by S100A7 via p65/NF?B-mediated Repression of ?Np63. Mol Cancer Res. 2017;15:1752-1763 pubmed publisher
    ..b>Implications: S100A7 is a new upstream regulator of the Hippo signaling pathway and reduces chemosensitivity of SCC cells through inhibitions of YAP expression and activity. Mol Cancer Res; 15(12); 1752-63. ©2017 AACR. ..
  92. Unsal D, Kacan M, Temiz Resitoglu M, Guden D, Korkmaz B, Sari A, et al. The role of Syk/I?B-?/NF-?B pathway activation in the reversal effect of BAY 61-3606, a selective Syk inhibitor, on hypotension and inflammation in a rat model of zymosan-induced non-septic shock. Clin Exp Pharmacol Physiol. 2017;: pubmed publisher
    ..alterations in the expression and/or activities of Syk, inhibitor ?B (I?B)-?, and nuclear factor-?B (NF-?B) p65. We also examined the effect of Syk inhibition on inducible nitric oxide synthase (iNOS), cyclooxygenase (COX)-2, ..