Genomes and Genes
Gene Symbol: GNAO1
Description: guanine nucleotide binding protein (G protein), alpha activating activity polypeptide O
Alias: G-ALPHA-o, GNAO, guanine nucleotide-binding protein G(o) subunit alpha
Publications122 found, 100 shown here
- A structural determinant that renders G alpha(i) sensitive to activation by GIV/girdin is required to promote cell migrationMikel Garcia-Marcos
Department of Cellular and Molecular Medicine, University of California, San Diego, La Jolla, California 92093, USA
J Biol Chem 285:12765-77. 2010....
- GAIP is membrane-anchored by palmitoylation and interacts with the activated (GTP-bound) form of G alpha i subunitsL de Vries
Division of Cellular and Molecular Medicine, University of California at San Diego, La Jolla 92093 0651, USA
Proc Natl Acad Sci U S A 93:15203-8. 1996..specifically with members of the G alpha i subfamily, G alpha i1, G alpha i2, G alpha i3, G alpha z, and G alpha o, but not with members of other G alpha subfamilies, G alpha s, G alpha q, and G alpha 12/13...
- Supersensitivity to mu-opioid receptor-mediated inhibition of the adenylyl cyclase pathway involves pertussis toxin-resistant Galpha protein subunitsRicardo Mostany
Departamento de Fisiologia y Farmacologia, Facultad de Medicina, Universidad de Cantabria, Av Herrera Oria s n, E 39011 Santander, Spain
Neuropharmacology 54:989-97. 2008..35S]GTP gamma S binding, mu-agonist-induced adenylyl cyclase inhibition, and co-precipitation of G alpha o, G alpha i2 G alpha z and G alpha q11 subunits with mu-opioid receptors...
- M1 muscarinic receptor signaling in mouse hippocampus and cortexAmy C Porter
Lilly Research Laboratories, Neuroscience Research Division, Eli Lilly and Company, Lilly Corporate Center, Indianapolis, IN 46285 0510, USA
Brain Res 944:82-9. 2002..These findings support a role for the M1 receptor subtype as the primary G(alpha)(q)/11-coupled muscarinic receptor in mouse hippocampus and cortex...
- Identification of protein pheromones that promote aggressive behaviourPablo Chamero
Department of Cell Biology, The Scripps Research Institute, La Jolla, California 92030, USA
Nature 450:899-902. 2007..a sensory neuron subfamily characterized by the expression of the G-protein Galpha(o) subunit (also known as Gnao) and Vmn2r putative pheromone receptors (V2Rs)...
- Microarray screening of lymphocyte gene expression differences in a multiplex schizophrenia pedigreeMarquis P Vawter
Department of Psychiatry and Human Behavior, College of Medicine, University of California, Irvine, CA 92697 1675, USA
Schizophr Res 67:41-52. 2004..3-q32) and the human guanine nucleotide-binding regulatory protein Go-alpha (GNAO1 localized at 16q13) was significantly decreased in individuals with schizophrenia compared to unaffected family ..
- 17beta-Oestradiol stimulation of G-proteins in aged and Alzheimer's human brain: comparison with phytoestrogensV Jefremov
Department of Biochemistry, Tartu University, Tartu, Estonia
J Neuroendocrinol 20:587-96. 2008..Both of these mechanisms could be involved in the neuroprotective signalling of oestrogens that contributes to their preventive/therapeutic action against postmenopausal neurological disorders...
- Involvement of G(q/11) in signal transduction in the mammalian vomeronasal organKennedy S Wekesa
Alabama State University, Biomedical Research and Training Programs, Montgomery, AL 36104 0271, USA
J Exp Biol 206:827-32. 2003..Our observations indicate that pheromones in male urine act on vomeronasal neurons in the female VNO via a receptor-mediated, G alpha(q/11)-protein-dependent increase in IP(3) levels...
- Changes of the level of G protein alpha-subunit mRNA by tolerance to and withdrawal from pentobarbital in ratsYounghwa Kim
Department of Anatomy, College of Medicine, Ewha Womans University, Seoul 158 710, Korea
Neurochem Res 27:527-33. 2002..showed that the level of G alpha s mRNA was increased in the septum and brainstem, and the level of G alpha o mRNA was elevated in the cortex during the pentobarbital withdrawal...
- Coupling of metabotropic glutamate receptors 2 and 4 to G alpha 15, G alpha 16, and chimeric G alpha q/i proteins: characterization of new antagonistsJ Gomeza
UPR Centre Nationale de Recherche Scientifique 9023, Mecanismes Moleculaires des Communications Cellulaires, CCIPE, Montpeller, France
Mol Pharmacol 50:923-30. 1996..Furthermore, the exchange of a few carboxyl-terminal residues of G alpha q by those of G alpha 12 or G alpha o allows the resulting chimeric G alpha subunits (G alpha ql and G alpha qol respectively) to couple Gi-coupled ..
- Starvation induces cAMP response element-binding protein-dependent gene expression through octopamine-Gq signaling in Caenorhabditis elegansSatoshi Suo
Department of Psychiatry, Institute of Medical Science, University of Toronto, Toronto, Ontario, Canada M5T 1R8
J Neurosci 26:10082-90. 2006..The results show that the endogenous octopamine system in C. elegans is activated by starvation and that different environmental stimuli can activate CREB through G alpha(q)...
- G alpha o mediates WNT-JNK signaling through dishevelled 1 and 3, RhoA family members, and MEKK 1 and 4 in mammalian cellsRama Kamesh Bikkavilli
Department of Pharmacology, Health Sciences Center, State University of New York at Stony Brook, Stony Brook, NY 11794 8651, USA
J Cell Sci 121:234-45. 2008..The canonical WNT-beta-catenin signaling pathway requires both G alpha o and G alpha q heterotrimeric G-proteins, whereas we show that WNT-JNK signaling requires only G alpha o protein...
- PAR1 and PAR2 couple to overlapping and distinct sets of G proteins and linked signaling pathways to differentially regulate cell physiologyKelly L McCoy
Department of Pharmacology, Rollins Research Center, Emory University School of Medicine, Atlanta, GA 30322, USA
Mol Pharmacol 77:1005-15. 2010..These studies demonstrate that PAR1 and PAR2 physically and functionally link to overlapping and distinct profiles of G proteins to differentially regulate downstream signaling pathways and cell physiology...
- Enhanced calcium cycling and contractile function in transgenic hearts expressing constitutively active G alpha o* proteinMing Zhu
Division of Cardiology, Rhode Island Hospital and The Warren Alpert Medical School of Brown University, Providence, RI 02903, USA
Am J Physiol Heart Circ Physiol 294:H1335-47. 2008..To investigate the role of G alpha o in the heart, we generated transgenic mice with cardiac-specific expression of a constitutively active form of G ..
- Formyl peptide receptors are candidate chemosensory receptors in the vomeronasal organStephen D Liberles
Howard Hughes Medical Institute, Basic Sciences Division, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA
Proc Natl Acad Sci U S A 106:9842-7. 2009..Given the presence of formylated peptides in bacteria and mitochondria, possible roles for VNO FPRs include the assessment of conspecifics or other species based on variations in normal bacterial flora or mitochondrial proteins...
- Expression of G-protein alpha subunit genes in the vestibular periphery of Rattus norvegicus and their chromosomal mappingJoseph A Cioffi
Department of Otolaryngology and Communication Sciences, Medical College of Wisconsin, Milwaukee, Wisconsin 53226, USA
Acta Otolaryngol 123:1027-34. 2003..The purpose of this study was to better define the role of these proteins by examining their expression in the rat vestibular periphery and characterizing their chromosomal location...
- RGS8 accelerates G-protein-mediated modulation of K+ currentsO Saitoh
Department of Molecular and Cellular Neurobiology, Tokyo Metropolitan Institute for Neuroscience, Japan
Nature 390:525-9. 1997..This property of RGS8 may be crucial for the rapid regulation of neuronal excitability upon stimulation of G-protein-coupled receptors...
- Localization of G protein alpha subunits and morphology of receptor neurons in olfactory and vomeronasal epithelia in Reeve's turtle, Geoclemys reevesiiYoshihiro Wakabayashi
Laboratory of Cell Biology and Anatomy, Department of Neuroscience Basic Technology, Tokyo Metropolitan Institute for Neuroscience, Fuchu, Tokyo 183 8526, Japan
Zoolog Sci 25:178-87. 2008..In rodents, signal transduction pathways involve G alpha olf and G alpha i2/G alpha o in OE and VNE, respectively. Reeve's turtles (Geoclemys reevesii) live in a semiaquatic environment...
- G protein subunit G gamma 13 is coexpressed with G alpha o, G beta 3, and G beta 4 in retinal ON bipolar cellsLiquan Huang
Department of Physiology and Biophysics, Mount Sinai School of Medicine, New York, New York 10029, USA
J Comp Neurol 455:1-10. 2003..Gbeta1, 2, and 5 are expressed in partially overlapping subpopulations of cone bipolar cells. Ggamma13 and Gbeta3 and/or Gbeta4, thus, seem selectively to participate in signal transduction by ON bipolar cells...
- Signal-transducing G proteins and antidepressant drugs: evidence for modulation of alpha subunit gene expression in rat brainK P Lesch
Section of Neuropharmacology, National Institute of Mental Health, Bethesda, Maryland
Biol Psychiatry 32:549-79. 1992..long-term administration of a wide spectrum of antidepressant drugs regulate G alpha s, G alpha i1, G alpha i2, G alpha o, G alpha q, and G alpha 12 mRNA and protein expression in various areas of the rat brain...
- Both G i and G o heterotrimeric G proteins are required to exert the full effect of norepinephrine on the beta-cell K ATP channelYing Zhao
Department of Molecular Medicine, College of Veterinary Medicine, Cornell University, Ithaca, New York 14853 6401, USA
J Biol Chem 283:5306-16. 2008..antisera raised against alpha and beta subunits (anti-G alpha common, anti-G beta, anti-G alpha i1/2/3, and anti-G alpha o) were used. Anti-G alpha common totally blocked the effects of NE on membrane potential and K ATP channels...
- Expression of G proteins in human placentas from pregnancies complicated by gestational hypertensionA Petit
Department of Obstetrics and Gynecology, University of Montreal, Research Center, Ste Justine Hospital, Quebec, Canada
Life Sci 60:953-60. 1997..Specifically, G alpha i2, G alpha i3, G alpha o, G alpha s, and G alpha q/11 levels reached 87%, 81%, 91%, 99%, and 103% respectively of those from normal ..
- Serotonin-mediated palmitoylation and depalmitoylation of G alpha proteins in rat brain cortical membranesS Bhamre
Laboratory of Molecular Pharmacology, Department of Pharmacology, MCP Hahnemann School of Medicine, Allegheny University of the Health Sciences, Philadelphia, Pennsylvania, USA
J Pharmacol Exp Ther 286:1482-9. 1998....
- Reductions in binding and functions of D2 dopamine receptors in the rat ventral striatum during amphetamine sensitizationJ C Chen
Department of Pharmacology, Chang Gung University, Tao Yuan, Taiwan, ROC
Life Sci 64:343-54. 1999..The decreases in D2 receptor function were unrelated to the coupled G-proteins, since none of the G alpha i-3, G alpha o or G alpha q in the ventral striatum exhibited quantitative differences between control and amphetamine ..
- Expression of a vomeronasal receptor gene (V1r) and G protein alpha subunits in goat, Capra hircus, olfactory receptor neuronsYoshihiro Wakabayashi
Laboratory of Cell Biology and Anatomy, Department of Neuroscience Basic Technology, Tokyo Metropolitan Institute for Neuroscience, Tokyo 183 8526, Japan
J Comp Neurol 503:371-80. 2007..by ORNs, whereas two vomeronasal receptor families (V1rs and V2rs) coupled respectively to G alpha i2 and G alpha o, are expressed by VRNs. Previously, we reported that one goat V1rs (gV1ra1) is expressed by ORNs and VRNs...
- Muscarinic M-current inhibition via G alpha q/11 and alpha-adrenoceptor inhibition of Ca2+ current via G alpha o in rat sympathetic neuronesM P Caulfield
Wellcome Laboratory for Molecular Pharmacology, Department of Pharmacology, University College London
J Physiol 477:415-22. 1994..2. Antibodies specific for G alpha q/11, but not those for G alpha o, reduced M-current inhibition by the muscarinic agonist oxotremorine-M, whereas anti-G alpha o antibodies, but ..
- Involvement of cholesterol in synaptic vesicle swellingJin Sook Lee
Department of Physiology, Wayne State University School of Medicine, Detroit, MI 48201, USA
Exp Biol Med (Maywood) 235:470-7. 2010..Our study further demonstrates that this effect of cholesterol is due, in part, to its involvement in the interactions between AQP-6, vH(+)-ATPase and the GTP-binding G(alpha o) protein at the synaptic vesicle membrane...
- Rapid plasma membrane anchoring of newly synthesized p59fyn: selective requirement for NH2-terminal myristoylation and palmitoylation at cysteine-3W van't Hof
Cell Biology and Genetics Program, Sloan Kettering Institute for Cancer Research, New York, NY 10021, USA
J Cell Biol 136:1023-35. 1997..These results serve to define a novel trafficking pathway for Src family proteins that are regulated by dual fatty acylation...
- Modest cholinergic deafferentation fails to alter hippocampal G-proteinsM McLaughlin
Neuroscience Institute, Department of Pharmacology and Neuroscience, Ninewells Medical School, University of Dundee, UK
Neurochem Int 35:59-64. 1999....
- G proteins activate ATP-sensitive K+ channels by antagonizing ATP-dependent gatingA Terzic
Department of Internal Medicine, Mayo Clinic, Rochester, Minnesota 55905
Neuron 12:885-93. 1994..Active forms of exogenous G protein subunits (G alpha i-1, G alpha i-2, or G alpha o) activated only KATP channels closed by ATPi...
- The G alpha(o/i)-coupled cannabinoid receptor-mediated neurite outgrowth involves Rap regulation of Src and Stat3John Cijiang He
Department of Pharmacology, Mount Sinai School of Medicine, New York, New York 10029, USA
J Biol Chem 280:33426-34. 2005..Overall, this study demonstrated that G alpha(o/i)-coupled CB1R triggers neurite outgrowth in Neuro-2A through the activation of a signaling network containing two pathways that bifurcate at Src and converge at Stat3...
- Cannabinoid receptor-induced neurite outgrowth is mediated by Rap1 activation through G(alpha)o/i-triggered proteasomal degradation of Rap1GAPIIJ Dedrick Jordan
Department of Pharmacology and Biological Chemistry, Mount Sinai School of Medicine, New York, New York 10029, USA
J Biol Chem 280:11413-21. 2005..Thus, the G(alpha)o/i-coupled cannabinoid receptor, by regulating the proteasomal degradation of Rap1GAPII, activates Rap1 to induce neurite outgrowth...
- Distribution of the alpha-subunit of the guanine nucleotide-binding protein Gi2 and its comparison to G alpha oJ C Lang
Abt Neuropharmakologie, Max Planck Institut fur Psychiatrie, Martinsried, FRG
J Recept Res 9:313-29. 1989..i2 was investigated in plasma membranes from different tissues and cells and compared to the distribution of G alpha o and other pertussis toxin sensitive G alpha...
- Gnao1 (G alphaO protein) is a likely genetic contributor to variation in physical dependence on opioids in miceB Kest
Department of Psychology and Center for Developmental Neuroscience, The College of Staten Island, City University of New York, Staten Island, NY 10314, USA
Neuroscience 162:1255-64. 2009..The most salient candidate gene within this QTL, Gnao1 (guanine nucleotide binding protein, alpha(o); G alpha(o); 96...
- Molecular cloning and sequence determination of four different cDNA species coding for alpha-subunits of G proteins from Xenopus laevis oocytesJ Olate
Departamento de Bioquimica, Facultad de Medicina, Universidad de Chile, Santiago
FEBS Lett 268:27-31. 1990..In addition to a clone coding for a G alpha o-type subunit previously reported [(1989) FEBS Lett...
- Developmental expression of G alpha o and G alpha s isoforms in PC12 cells: relationship to neurite outgrowthS Andreopoulos
Section of Biochemical Psychiatry, Clarke Institute of Psychiatry, University of Toronto, Ont, Canada
Brain Res Dev Brain Res 88:30-6. 1995..Furthermore, no significant changes were observed in the levels of alpha o or alpha s isoforms following NGF removal. These findings suggest that alpha o isoforms are involved in neuritic extension in PC12 cells...
- The trimeric G protein Go inflicts a double impact on axin in the Wnt/frizzled signaling pathwayDiane Egger-Adam
Department of Biology, University of Konstanz, Konstanz, Germany
Dev Dyn 239:168-83. 2010..b>G alpha o physically binds Axin and re-localizes it to the plasma membrane...
- Oxidized human neuroglobin acts as a heterotrimeric Galpha protein guanine nucleotide dissociation inhibitorKeisuke Wakasugi
Department of Molecular Engineering, Graduate School of Engineering, Kyoto University, Japan
J Biol Chem 278:36505-12. 2003..In contrast, ferrous ligand-bound Ngb under normoxia did not have GDI activities. Taken together, we propose that human Ngb may be a novel oxidative stress-responsive sensor for signal transduction in the brain...
- Ret-PCP2 colocalizes with protein kinase C in a subset of primate ON cone bipolar cellsPyroja Sulaiman
Department of Neuroscience, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
J Comp Neurol 518:1098-112. 2010....
- Mechanosensory inputs influence Caenorhabditis elegans pharyngeal activity via ivermectin sensitivity genesJohn Keane
Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, Texas 75390 9148, USA
Genetics 164:153-62. 2003..Tail tap of dauers stimulates pumping through a mechanism involving G alpha o and G alpha q...
- Functional characterization of human receptors for short chain fatty acids and their role in polymorphonuclear cell activationEmmanuel Le Poul
Euroscreen, Rue Adrienne Bolland 47, 6041 Gosselies, Laboratory of Molecular Immunology, Rega Institute for Medical Research, Catholic University of Leuven, Minderbroedersstraat 10, B 3000 Leuven, Belgium
J Biol Chem 278:25481-9. 2003..GPR43 might therefore constitute a target allowing us to modulate immune responses in these pathological situations...
- Coregulation of natively expressed pertussis toxin-sensitive muscarinic receptors with G-protein-activated potassium channelsSinead M Clancy
Peptide Biology Laboratory, The Salk Institute for Biological Studies, La Jolla, California 92037, USA
J Neurosci 27:6388-99. 2007..This new pathway for controlling Kir3 signaling could help limit the potential harmful effects of excessive Kir3 activity in the brain...
- An N-terminal region of Caenorhabditis elegans RGS proteins EGL-10 and EAT-16 directs inhibition of G(alpha)o versus G(alpha)q signalingGeorgia A Patikoglou
Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut 06520 8024, USA
J Biol Chem 277:47004-13. 2002..We conclude that the N-terminal region directs inhibition of G(alpha) signaling by forming a complex with the GGL/RGS region and affecting its stability, membrane localization, and G(alpha) target specificity...
- Diverse somatic mutation patterns and pathway alterations in human cancersZhengyan Kan
Department of Molecular Biology, Genentech Inc, 1 DNA Way, South San Francisco, California 94080, USA
Nature 466:869-73. 2010..Furthermore, our experimental analyses demonstrate the functional roles of mutant GNAO1 (a Galpha subunit) and mutant MAP2K4 (a member of the JNK signalling pathway) in oncogenesis...
- [Binding of G alpha o N-terminus is responsible for the voltage-resistant inhibition of alpha 1A (P/Q-type, Cav2.1) Ca2+ channels]Mariko Kinoshita
Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo ku, Kyoto 606 8501, Japan
Yakugaku Zasshi 122:565-72. 2002..Here, we show that the N-terminus of G alpha o is critical for the interaction with the C-terminus of the P/Q-type channel subunit, and that the binding ..
- Molecular basis of a novel oncogenic mutation in GNAO1M Garcia-Marcos
Department of Cellular and Molecular Medicine, San Diego School of Medicine, University of California, La Jolla, CA 92093 0651, USA
Oncogene 30:2691-6. 2011..Recently the first somatic mutation, arginine(243) → histidine (R243H) in the GNAO1 (Gαo) gene was identified in breast carcinomas and shown to promote oncogenic transformation when introduced into ..
- Thrombin receptors activate G(o) proteins in endothelial cells to regulate intracellular calcium and cell shape changesJurgen F Vanhauwe
Institute for Neuroscience, Department of Molecular Pharmacology and Biological Chemistry, Northwestern University, Chicago, IL 60611, USA
J Biol Chem 277:34143-9. 2002..We conclude that the G(o) proteins play a role in thrombin signaling distinct from G(i1/2) proteins, which are mediated through their Gbetagamma subunits and involve coupling to calcium signaling and cytoskeletal rearrangements...
- Evidence for protean agonism of RX 831003 at alpha 2A-adrenoceptors by co-expression with different G alpha protein subunitsP J Pauwels
Centre de Recherche Pierre Fabre, Department of Cellular and Molecular Biology, 17, avenue Jean Moulin, 81106 Castres Cedex, France
Neuropharmacology 42:855-63. 2002..These data indicate that the alpha(2) AR ligand RX 831003 behaves as a protean agonist at the alpha(2A) AR and that its activity is highly dependent on the co-expressed G(alpha) protein subunit...
- Serine 232 of the alpha(2A)-adrenergic receptor is a protein kinase C-sensitive effector coupling switchM Liang
Department of Medicine, University of Cincinnati College of Medicine, 231 Albert Sabin Way, ML 0564, Cincinnati, Ohio 45267 0564, USA
Biochemistry 40:15031-7. 2001..This represents one mechanism by which cells route stimuli directed to multifunctional receptors to selected effectors so as to attain finely targeted signaling...
- A novel function of Goalpha: mediation of extracellular signal-regulated kinase activation by opioid receptors in neural cellsWen Bo Zhang
Laboratory of Molecular Cell Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 320 Yue Yang Road, Shanghai 200031, China
J Neurochem 86:1213-22. 2003..This study suggests that one of the functions of Goalpha in the brain is to mediate extracellular signal-regulated kinase activation by G protein-coupled receptors...
- CKbeta8/CCL23 and its isoform CKbeta8-1 induce up-regulation of cyclins via the G(i)/G(o) protein/PLC/PKCdelta/ERK leading to cell-cycle progressionJeonghan Kim
Korea University, Seoul, South Korea
Cytokine 50:42-9. 2010..These results indicate that both CKbeta8 and CKbeta8-1 are involved in cell proliferation by modulating the cell cycle regulators...
- Verification of proposed peripheral biomarkers in mononuclear cells of individuals with schizophreniaYuanrong Yao
Department of Neurology, Tianjin Medical University General Hospital, Anshan Avenue 154, 300052 Tianjin, China
J Psychiatr Res 42:639-43. 2008..In light of previous reports, expression levels of the following genes: APOBEC3B, CXCL1, DRD2, GNAO1, Kir2...
- Galphao/i-stimulated proteosomal degradation of RGS20: a mechanism for temporal integration of Gs and Gi pathwaysMario Pagano
Department of Pharmacology and Biological Chemistry, Mount Sinai School of Medicine, New York, NY 10029, United States
Cell Signal 20:1190-7. 2008..These observations suggest that G(o/i)-coupled receptors, by stimulating the degradation of RGS20, can regulate how subsequent activation of the G(s) and G(i) pathways controls cellular cAMP levels, thus allowing for signal integration...
- A specific subset of transient receptor potential vanilloid-type channel subunits in Caenorhabditis elegans endocrine cells function as mixed heteromers to promote neurotransmitter releaseAntony M Jose
Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut 06520, USA
Genetics 175:93-105. 2007....
- Generation of RGS8 null mutant mice by Cre/loxP systemHitoshi Kuwata
Division of Molecular Genetics, Department of Physiology and Cell Biology, Kobe University Graduate School of Medicine, Kobe 650 0017, Japan
Kobe J Med Sci 53:275-81. 2007..RGS8 binds specifically to G alpha o and G alpha i3 in vitro and activates their intrinsic GTPase activities...
- Major sperm protein signaling promotes oocyte microtubule reorganization prior to fertilization in Caenorhabditis elegansJana E Harris
Department of Cell and Developmental Biology, Vanderbilt University School of Medicine, Nashville, TN 37232, USA
Dev Biol 299:105-21. 2006..We propose that MSP-dependent microtubule reorganization promotes meiotic spindle assembly by facilitating the search and capture of microtubules by meiotic chromatin following NEBD...
- Mechanism of the receptor-catalyzed activation of heterotrimeric G proteinsWilliam M Oldham
Department of Pharmacology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232 6600, USA
Nat Struct Mol Biol 13:772-7. 2006..These data provide the first direct observation of R*-mediated conformational changes in G proteins and define the structural basis for GDP release from G alpha...
- Expression of nonclassical class I major histocompatibility genes defines a tripartite organization of the mouse vomeronasal systemTomohiro Ishii
The Rockefeller University, New York, New York 10065, USA
J Neurosci 28:2332-41. 2008..Our results reveal a tripartite organization of the VNO and AOB, question the generality of the requirement of these nine H2-Mv molecules for V2R surface expression, and suggest that H2-Mvs can function in both dendrites and axons...
- G betagamma binds histone deacetylase 5 (HDAC5) and inhibits its transcriptional co-repression activityBryan D Spiegelberg
Department of Pharmacology, Vanderbilt University, Nashville, Tennessee 37232, USA
J Biol Chem 280:41769-76. 2005..Taken together, these data imply that HDAC5 and possibly other class II HDACs can be added to the growing list of G betagamma effectors...
- G protein G(alpha)o is essential for vomeronasal function and aggressive behavior in micePablo Chamero
Department of Physiology, University of Saarland School of Medicine, 66424 Homburg, Germany
Proc Natl Acad Sci U S A 108:12898-903. 2011..We have used gene targeting to examine the role of the G protein Gαo, encoded by the gene Gnao1, in vomeronasal function...
- Retina-specific GTPase accelerator RGS11/G beta 5S/R9AP is a constitutive heterotrimer selectively targeted to mGluR6 in ON-bipolar neuronsYan Cao
Department of Pharmacology, University of Minnesota, Minneapolis, Minnesota 55455, USA
J Neurosci 29:9301-13. 2009..These results suggest that the deactivation of mGluR6 cascade during the light response may require the contribution of multiple GTPase activating proteins...
- Mutations that rescue the paralysis of Caenorhabditis elegans ric-8 (synembryn) mutants activate the G alpha(s) pathway and define a third major branch of the synaptic signaling networkMichael A Schade
Program in Molecular, Cell and Developmental Biology, Oklahoma Medical Research Foundation, Oklahoma City, Oklahoma 73104, USA
Genetics 169:631-49. 2005..Our results appear to link RIC-8 (synembryn) and a third major G alpha pathway, the G alpha(s) pathway, with the previously discovered G alpha(o) and G alpha(q) pathways of the synaptic signaling network...
- 5-HT1 receptorsLaurence Lanfumey
INSERM U 288, Faculté de Médecine Pitié Salpêtrière, 91, Boulevard de l Hopital, 75013 Paris, France
Curr Drug Targets CNS Neurol Disord 3:1-10. 2004..All the 5-HT(1) receptor types actually interact with G alpha i/G alpha o proteins to inhibit adenylyl cyclase and modulate ionic effectors, i.e. potassium and/or calcium channels...
- Transcriptional profiling of septal wall of the right ventricular outflow tract in patients with idiopathic ventricular arrhythmiasCan Hasdemir
Department of Cardiology, Ege University School of Medicine, Bornova, Izmir, Turkey
Pacing Clin Electrophysiol 33:159-67. 2010..The aim of this study was to investigate the genome-wide transcriptional profiling of the septal wall of the RVOT in patients with RVOT tachycardia and control subjects...
- Endogenous melanin-concentrating hormone receptor SLC-1 in human melanoma SK-MEL-37 cellsY Saito
Department of Pharmacology, Saitama Medical School, Morohongo, Saitama 350 0495, Japan
Biochem Biophys Res Commun 289:44-50. 2001..This study provides for the first time a skin-derived cellular model to analyze the molecular mechanism of the MCH signaling pathway...
- Effects of neonatal hypothyroidism on the expressions of growth cone proteins and axon guidance molecules related genes in the hippocampusC C Wong
Department of Physiology, The Chinese University of Hong Kong, Shatin, N T, Hong Kong
Mol Cell Endocrinol 184:143-50. 2001..transcription of beta-actin, sema 3F, CRMP 1 to 4, GAP-43, G alpha o1, G alpha o2 and translation of beta-actin, G alpha o, G alpha o1, CRMP-2, CRMP-4 genes were examined in the hippocampus of neonatal methimazole induced hypothyroid ..
- Phospholipase C-beta 1 is a GTPase-activating protein for Gq/11, its physiologic regulatorG Berstein
Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas 75235 9041
Cell 70:411-8. 1992..Such GAP activity by an effector coupled to a trimeric G protein can reconcile slow GTP hydrolysis by pure G proteins in vitro with fast physiologic deactivation of G protein-mediated signaling...
- Functional characterisation of the Drosophila 5-HTdro1 and 5-HTdro2B serotonin receptors in insect cells: activation of a G(alpha) s-like protein by 5-HTdro1 but lack of coupling to inhibitory G-proteins by 5-HTdro2BL A Obosi
School of Biological and Molecular Sciences, Oxford Brookes University, UK
FEBS Lett 381:233-6. 1996..Immunoblot analysis of the endogenous G-proteins revealed that Sf9 cells lack the G-protein subtypes G(alpha i 1-3) and G(alpha)o, but express the subtype G(alpha)s and G(alpha)q...
- Effect of thyroid deficiency on Go alpha-subunit isoforms in developing rat cerebral cortexP P Li
Section of Biochemical Psychiatry, Clarke Institute of Psychiatry, University of Toronto, Ontario, Canada
Neurochem Res 20:1249-55. 1995Postnatal development of G alpha o isoforms in rat cerebral cortex was studied by SDS-PAGE and immunoblotting...
- Evidence for the coupling of Gq protein to D1-like dopamine sites in rat striatum: possible role in dopamine-mediated inositol phosphate formationH Y Wang
Department of Pharmacology, Medical College of Pennsylvania, Philadelphia 19129, USA
Mol Pharmacol 48:988-94. 1995..Furthermore, antisera directed against G alpha s or G alpha q but not G alpha i, G alpha o, or G alpha z precipitated specific D1-like binding sites labeled with [3H]SCH23390...
- Immunoexpression of the alpha subunit of a guanine nucleotide-binding protein (Go) in pulmonary neuroendocrine cells and neoplasmsN Kawano
Department of Pathology, Yokohama City University School of Medicine, Japan
Pathol Int 46:393-8. 1996..The biological significance of Go alpha is unclear in normal and neoplastic lung tissues, but Go alpha is a useful marker of neuroendocrine cells and neoplasma of the lung...
- Palmitoylation of p59fyn is reversible and sufficient for plasma membrane associationA Wolven
Program in Molecular Biology, Cornell University Graduate School of Medical Sciences, New York, New York 10021, USA
Mol Biol Cell 8:1159-73. 1997..Substitution of the SH4 motif within Fyn with heterologous sequences from other palmitoylated proteins (G alpha o and GAP43) revealed that the presence of palmitate is sufficient to direct plasma membrane localization ..
- A multigene family encoding a diverse array of putative pheromone receptors in mammalsH Matsunami
Howard Hughes Medical Institute, Department of Neurobiology, Harvard Medical School, Boston, Massachusetts 02115, USA
Cell 90:775-84. 1997..However, a dramatically different structure for VRs and the existence of variant VR mRNA forms indicate that there are diverse strategies to detect functionally distinct sensory stimuli...
- Analysis of the N-terminal binding domain of Go alphaL Busconi
Renal Division, Brigham and Women s Hospital, Harvard Medical School, Boston, MA 02115, USA
Biochem J 328:23-31. 1997..A detailed understanding of G alpha-membrane interactions may reveal unique mechanisms for regulating signal transduction...
- Reduced adenosine A1 receptor and G alpha protein coupling in rat ventricular myocardium during agingG Cai
Department of Pharmacology, Allegheny University of the Health Sciences, Philadelphia, PA 19129, USA
Circ Res 81:1065-71. 1997..ventricular membranes, specific [3H]DPCPX binding sites were detected in immunoprecipitates of G alpha i3 and G alpha o antisera but not with antibodies for other G alpha proteins...
- Analysis of G protein alpha subunit mRNA abundance in preimplantation mouse embryos using a rapid, quantitative RT-PCR approachL Rambhatla
Fels Institute for Cancer Research and Molecular Biology, Temple University School of Medicine, Philadelphia, Pennsylvania, USA
Mol Reprod Dev 41:314-24. 1995..we analyzed the patterns of expression of nine G protein alpha subunit mRNAs (G alpha s, G alpha i, G alpha q, G alpha o, and G alpha 11-15) in oocytes, eggs, and preimplantation embryos from fertilization to the blastocyst stage...
- The structure of the G protein heterotrimer Gi alpha 1 beta 1 gamma 2M A Wall
Department of Biochemistry, University of Texas Southwestern Medical Center, Dallas 75235, USA
Cell 83:1047-58. 1995..Repeated WD motifs in beta form a circularized sevenfold beta propeller. The conserved cores of these motifs are a scaffold for display of their more variable linkers on the exterior face of each propeller blade...
- Mutagenesis of the amino terminus of the alpha subunit of the G protein Go. In vitro characterization of alpha o beta gamma interactionsB M Denker
Department of Medicine, Brigham and Women s Hospital, Boston, Massachusetts
J Biol Chem 267:6272-7. 1992..Deletion of this region diminishes the ability of alpha o to interact with the beta gamma subunit, but substitutions at this position indicate that other amino acids can be tolerated without affecting subunit interaction...
- Identification of G protein alpha-subunits in RINm5F cells and their selective interaction with galanin receptorM Cormont
Unit 145, National Institute of Health and Medical Research, Faculty of Medicine, Nice, France
Diabetes 40:1170-6. 1991..at concentrations where there were no effects of other antibodies specifically directed against Gi alpha 3 or G alpha o. These data suggest that Gi1 and/or Gi2 interact with the galanin receptor and probably mediate the effects of ..
- Molecular cloning and DNA sequence analysis of the human guanine nucleotide-binding protein Go alphaS Lavu
Biological Response Modifiers Program, National Cancer Institute Frederick Cancer Research Facility, MD 21701
Biochem Biophys Res Commun 150:811-5. 1988..There is 100% identity at the amino acid level for the cholera and pertussis toxin-catalyzed ADP ribosylation sites, the putative guanine nucleotide binding, and the GTP hydrolysis sites...
- Voltage-dependent calcium channel beta-subunits in combination with alpha 1 subunits, have a GTPase activating effect to promote the hydrolysis of GTP by G alpha o in rat frontal cortexV Campbell
Department of Pharmacology, Royal Free Hospital School of Medicine, London, UK
FEBS Lett 370:135-40. 1995..This may be a mechanism by which calcium channels can normally act to limit the duration of a G-protein modulatory signal...
- Expression of GTP-binding protein alpha subunits in human thymocytesP S Kabouridis
Department of Biology, San Diego State University, CA 92182, USA
Mol Cell Biochem 144:45-51. 1995..The alpha subunit genes identified are: G alpha i1-3 and G alpha z but not G alpha o from the Gi family, G alpha s from the Gs family, G alpha 11, G alpha q, and G alpha 16 from the Gq family, and ..
- Association of heterotrimeric GTP binding regulatory protein (Go) with mitosisH C Wu
Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan, Republic of China
Lab Invest 71:175-81. 1994..In the present study, we have conducted experiments to examine whether the G alpha subunit is also associated with mitotic spindles...
- Identification of GTP-binding proteins in human glomeruliK Nitta
Department of Medicine, Kidney Center, Tokyo Women s Medical College, Japan
Nihon Jinzo Gakkai Shi 36:9-12. 1994..This is the first report that characterizes G-protein subunits in human glomeruli. Further study is required to determine the roles of G-proteins in signal transductions in human glomeruli...
- In situ hybridization reveals specific increases in G alpha s and G alpha o mRNA in discrete brain regions of morphine-tolerant ratsD Parolaro
Institute of Pharmacology, Faculty of Sciences, University of Milan, Italy
Eur J Pharmacol 244:211-22. 1993..significantly increased alpha s amounts in the hypothalamus (70%), and produced a minor elevation (30%) in G alpha o levels in the olfactory area...
- Expression of GTPase-deficient Gialpha2 results in translocation of cytoplasmic RGS4 to the plasma membraneK M Druey
Laboratory of Immunoregulation, National Institutes of Health, Bethesda, Maryland 20892 1876, USA
J Biol Chem 273:18405-10. 1998....
- Nrg-1 belongs to the endothelial differentiation gene family of G protein-coupled sphingosine-1-phosphate receptorsR L Malek
Department of Molecular and Cellular Biology, Institute for Genomic Research, Rockville, Maryland 20850, USA
J Biol Chem 276:5692-9. 2001..The antiproliferative effect, like S1P-mediated ERK1/2 inhibition, was orthovanadate-sensitive and pertussis toxin-insensitive. Our results indicate that EDG-8, a member of the EDG-1 subfamily, couples to unique signaling pathways...
- G alpha(i) and G alpha(o) are target proteins of reactive oxygen speciesM Nishida
Laboratory of Pharmacology and Toxicology, Graduate School of Pharmaceutical Science, University of Tokyo, Japan
Nature 408:492-5. 2000..We conclude that G alpha(i) and G alpha(o) are critical targets of oxidative stress for activation of ERK...
- Cloning and characterization of the human phosphoinositide-specific phospholipase C-beta 1 (PLC beta 1)A Caricasole
Biology Department, GlaxoWellcome Medicines Research Centre, Verona, Italy
Biochim Biophys Acta 1517:63-72. 2000..Finally, we characterized the structure of the PLC beta 1 locus and confirmed its mapping to human chromosome 20...
- Interaction between HIV-1 NEF and G(o) proteins in transfected COS-7 cellsF Guzzi
Department of Experimental and Environmental Medicine and Biotechnology, School of Medicine, University of Milano Bicocca, Via Cadore 48, Monza, 20052, Italy
Biochem Biophys Res Commun 270:570-5. 2000..We conclude that Nef interacts with nonpalmitoylated G(o)alpha, presumably outside caveolin-rich microdomains of the plasma membrane...
- Mammalian Ric-8A (synembryn) is a heterotrimeric Galpha protein guanine nucleotide exchange factorGregory G Tall
Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas 75390 9041, USA
J Biol Chem 278:8356-62. 2003..Ric-8A interacts with GDP-bound Galpha proteins, stimulates release of GDP, and forms a stable nucleotide-free transition state complex with the Galpha protein; this complex dissociates upon binding of GTP to Galpha...
- Kinetics of ternary complex formation with fusion proteins composed of the A(1)-adenosine receptor and G protein alpha-subunitsM Waldhoer
Institute of Pharmacology, University of Vienna, Währinger Strasse 13a, A 1090 Vienna, Austria
J Biol Chem 274:30571-9. 1999..These features provide for a kinetic proofreading mechanism that sustains the fidelity of receptor-G protein coupling...
- Ultrastructural localization of the GTP-binding protein Go in neuronsJ Gabrion
Laboratoire de Neurobiologie Endocrinologique, UA 1197 CNRS, Universite de Montpellier II, France
Cell Signal 1:107-23. 1989....
- Interaction of heterotrimeric G protein Galphao with Purkinje cell protein-2. Evidence for a novel nucleotide exchange factorY Luo
Renal Division, Department of Medicine, Brigham and Women s Hospital and Harvard Medical School, Boston, Massachusetts 02115, USA
J Biol Chem 274:10685-8. 1999..These findings define a novel nucleotide exchange function for Pcp2 and suggest that the interaction between Pcp2 and Galphao is important to Purkinje cell function...
- Activation of Go-proteins by membrane depolarization traced by in situ photoaffinity labeling of galphao-proteins with [alpha32P]GTP-azidoanilideY Anis
Department of Physiology and The Cardiac Research Institute, Sackler School of Medicine, Tel Aviv University, 69978 Tel Aviv, Israel
J Biol Chem 274:7431-40. 1999..These findings support a possible involvement of VGSC in depolarization-induced activation of Go-proteins...
- Norepinephrine exocytosis stimulated by alpha-latrotoxin requires both external and stored Ca2+ and is mediated by latrophilin, G proteins and phospholipase CM A Rahman
Biochemistry Department, Imperial College, London, UK
Philos Trans R Soc Lond B Biol Sci 354:379-86. 1999..As revealed by immunostaining, latrophilin interacts with G alpha q/11 and G alpha o but not with G alpha s, G alpha i or G alpha z, indicating that this receptor may couple to several G proteins ..
- Coincident signalling between the Gi/Go-coupled delta-opioid receptor and the Gq-coupled m3 muscarinic receptor at the level of intracellular free calcium in SH-SY5Y cellsA Yeo
Department of Pharmacology, University of Bristol, Bristol, UK
J Neurochem 76:1688-700. 2001....
- Analysis of the relative interactions between the alpha 2C10 adrenoceptor and the guanine-nucleotide-binding proteins G(o)1 alpha and Gi 2 alpha following co-expression of these polypeptides in rat 1 fibroblastsM A Grassie
Molecular Pharmacology Group, University of Glasgow, Scotland, U K
Biochem J 306:525-30. 1995....
- Factors determining the specificity of signal transduction by guanine nucleotide-binding protein-coupled receptors. II. Preferential coupling of the alpha 2C-adrenergic receptor to the guanine nucleotide-binding protein, GoI Coupry
Cellular and Molecular Research Laboratory, Massachusetts General Hospital, Harvard Medical School, Boston 02114
J Biol Chem 267:9852-7. 1992..This report indicates the utility of R-G cotransfection in mammalian cells as a "natural environment model" to characterize events occurring at the R-G and G-E interface...
- Up-regulation in late pregnancy of both Go1 alpha and Go2 alpha isoforms in human myometriumR Zumbihl
INSERM U65, Universite Montpellier II, France
Eur J Pharmacol 288:9-15. 1994....
- Different forms of Go alpha mRNA arise by alternative splicing of transcripts from a single gene on human chromosome 16J J Murtagh
Laboratory of Cellular Metabolism, National Heart, Lung, and Blood Institute, Bethesda, Maryland 20892
Mol Cell Biol 11:1146-55. 1991..The use of oligonucleotide probes of the type described here represents a new strategy, potentially widely applicable for mapping and elucidating structural features of genes...
- Pharmacological Targeting of Regulators of G Protein SignalingRichard Neubig; Fiscal Year: 2007..The ultimate goal is to define their potential as therapeutic targets in heart disease, obesity, and diabetes. ..
- Signaling Specificity of Drosophila Serpentine ReceptorsAndrew Tomlinson; Fiscal Year: 2007..3. We will use fly genetics to determine whether G-alpha-o also transduces the Wg signal, and also identify its molecular partners in the trimeric complex. ..
- Biomarker Genes in Mood Disorder: Lymphocyte and BrainMARQUIS VAWTER; Fiscal Year: 2006..The potential use of biomarkers in lymphocytes will provide clinical researchers a tool to better address questions concerning diagnostic subgroups and treatment responders and non-responders. ..
- G Protein Regulation of NHE ActivityJohn Raymond; Fiscal Year: 2005..We believe that we are uniquely poised to study these novel mechanisms of regulation of NHE- 1, and are enthusiastic about the prospects of continuing these studies. ..
- TRAINING GRANT IN GLOMERULAR FUNCTION AND DISEASEJohn Raymond; Fiscal Year: 2007..Our aim is to produce outstanding investigators with a broad insight into glomerular dysfunction, and who will make significant contributions to the understanding of progressive glomerular disorders. ..
- Mechanisms of Regulation Of NHE-1John Raymond; Fiscal Year: 2009..The work could lead to new therapies for hypertension, congestive heart failure, cardiomyopathies and kidney failure. ..
- Mitochondrial Abnormalities in Schizophrenia and Bipolar DisorderMARQUIS PHILIP VAWTER; Fiscal Year: 2010..By understanding the accumulation of mitochondrial DNA defects in brain, it will advance mitochondrial medicine for earlier diagnosis and treatment of brain related disorders. ..
- Mechanisms of Regulation Of NHE-1John R Raymond; Fiscal Year: 2010..The work could lead to new therapies for hypertension, congestive heart failure, cardiomyopathies and kidney failure. ..
- TRAINING GRANT IN GLOMERULAR FUNCTION AND DISEASEJohn Raymond; Fiscal Year: 2007..Our aim is to produce outstanding investigators with a broad insight into glomerular dysfunction, and who will make significant contributions to the understanding of progressive glomerular disorders. ..
- Mechanisms of Regulation Of NHE-1John Raymond; Fiscal Year: 2007..This proposal could also lay the foundation for future structural experiments utilizing NMR and/or crystallography methods. ..
- Regulation of EGF Receptors by G protein receptorJohn Raymond; Fiscal Year: 2004..These studies address important gaps in our knowledge of GPCR signals that regulate the functions of RTK's. ..
- Human Subjects Research Enhancements Program at MUSCJohn Raymond; Fiscal Year: 2003..abstract_text> ..
- EXTRAMURAL RESEARCH FACILITIEIS CONSTRUCTIONJohn Raymond; Fiscal Year: 2002..Total project cost is $4,212,617, of which the University's share is 53 percent. ..
- GI-REGULATED NHE ACTIVITY IN RENAL AND FIBROBLAST CELLSJohn Raymond; Fiscal Year: 2001....
- EXTRAMURAL RESEARCH FACILITIES CONSTRUCTIONJohn Raymond; Fiscal Year: 2003..The total project cost is $6,342,887; the University is contributing $3,171,443 (50 percent). ..
- NADPH OXIDASE & MESANGIAL CELL PROLIFERATION & FIBROSISJohn Raymond; Fiscal Year: 2003..Number 2. To establish a role for ROS generated by NAD(P)H oxidase in an animal model of proliferative and fibrotic renal disease. ..
- Human Subjects Research Enhancements Program at MUSCJohn Raymond; Fiscal Year: 2002..abstract_text> ..