GNAI3

Summary

Gene Symbol: GNAI3
Description: guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 3
Alias: 87U6, ARCND1, g(i) alpha-3, guanine nucleotide-binding protein G(k) subunit alpha
Species: human

Top Publications

  1. ncbi Distinct guanine nucleotide binding and release properties of the three Gi proteins
    D J Carty
    Department of Pharmacology, Mount Sinai School of Medicine, City University, New York, New York 10029
    J Biol Chem 265:6268-73. 1990
  2. ncbi RGS10 is a selective activator of G alpha i GTPase activity
    T W Hunt
    Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts 02138, USA
    Nature 383:175-7. 1996
  3. ncbi Structural basis for the selectivity of the RGS protein, GAIP, for Galphai family members. Identification of a single amino acid determinant for selective interaction of Galphai subunits with GAIP
    D S Woulfe
    Department of Pharmacology, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
    J Biol Chem 274:17718-24. 1999
  4. ncbi Changes in expression of adenyl cyclase activity in human endometrium during hormone replacement therapy and ovarian stimulation
    L Bernardini
    Department of Obstetrics and Gynaecology, University of Genoa, Genoa, Italy
    Mol Hum Reprod 5:955-60. 1999
  5. pmc Variations in Gnai2 and Rgs1 expression affect chemokine receptor signaling and the organization of secondary lymphoid organs
    I Y Hwang
    B Cell Molecular Immunology Section, Laboratory of Immunoregulation, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    Genes Immun 11:384-96. 2010
  6. pmc GAIP, a protein that specifically interacts with the trimeric G protein G alpha i3, is a member of a protein family with a highly conserved core domain
    L de Vries
    Division of Cellular and Molecular Medicine, University of California, San Diego, La Jolla 92093 0651, USA
    Proc Natl Acad Sci U S A 92:11916-20. 1995
  7. pmc GAIP is membrane-anchored by palmitoylation and interacts with the activated (GTP-bound) form of G alpha i subunits
    L de Vries
    Division of Cellular and Molecular Medicine, University of California at San Diego, La Jolla 92093 0651, USA
    Proc Natl Acad Sci U S A 93:15203-8. 1996
  8. pmc Galpha i3 binding to calnuc on Golgi membranes in living cells monitored by fluorescence resonance energy transfer of green fluorescent protein fusion proteins
    T S Weiss
    Department of Cellular and Molecular Medicine, University of California at San Diego, La Jolla, CA 92093, USA
    Proc Natl Acad Sci U S A 98:14961-6. 2001
  9. pmc Gialpha and Gbeta subunits both define selectivity of G protein activation by alpha2-adrenergic receptors
    Scott K Gibson
    Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA
    Proc Natl Acad Sci U S A 103:212-7. 2006
  10. ncbi Regional localization of the human G protein alpha i2 (GNAI2) gene: assignment to 3p21 and a related sequence (GNAI2L) to 12p12-p13
    I Magovcevic
    Department of Genetics, Brigham and Women s Hospital, Harvard Medical School, Boston, Massachusetts 02115
    Genomics 12:125-9. 1992

Research Grants

  1. Structure and mechanism of beta2-adrenergic receptor
    Roger Sunahara; Fiscal Year: 2007
  2. GPCR signaling complexes in living cells
    Nevin Lambert; Fiscal Year: 2009
  3. GPCR signaling complexes in living cells
    NEVIN ALAN LAMBERT; Fiscal Year: 2010
  4. GPCR signaling complexes in living cells
    Nevin Lambert; Fiscal Year: 2009
  5. Regulation of G-protein signaling in CNS neurons
    Nevin Lambert; Fiscal Year: 2006
  6. CALCIUM AND ENDOCYTOTIC MEMBRANE RETRIEVAL
    Nevin Lambert; Fiscal Year: 2003
  7. GPCR signaling complexes in living cells
    NEVIN ALAN LAMBERT; Fiscal Year: 2010

Scientific Experts

  • F Toledo
  • Sara Ferrando
  • Scott K Gibson
  • Roger Sunahara
  • O Saitoh
  • Christopher T Gordon
  • A Sidhu
  • Yonghong Li
  • Hyeseon Cho
  • Kyung-Hee Rhee
  • Francis S Willard
  • Yuren Wang
  • P G Burgon
  • D Yowe
  • S Miotti
  • Nevin Lambert
  • Mikel Garcia-Marcos
  • F L Zhang
  • Lutz Birnbaumer
  • Il Young Hwang
  • Wen Qing Li
  • Nicholas W Plummer
  • Mark J Rieder
  • Eduard Stefan
  • Shai Berlin
  • J Shi
  • Alejandra Young
  • L de Vries
  • Dana Baram
  • I Y Hwang
  • Benoit Laliberte
  • Ali Vural
  • J H Kehrl
  • Robert S Flynn
  • Pradipta Ghosh
  • William M Oldham
  • Caroline Marty
  • Hyung Ki Lee
  • Qingjiong Zhang
  • M G Farquhar
  • Anja Mueller
  • P Lin
  • Rachna Kaushik
  • Keisuke Wakasugi
  • Gregory G Tall
  • Thierry Fischer
  • Alison W Gagnon
  • Margaret A Tucker
  • Chung Park
  • Sanford M Dawsey
  • Stephen J Chanock
  • Zhaoming Wang
  • Xiaoqin Xiong
  • Yi Xu
  • Gwen Murphy
  • John H Kehrl
  • Chaoyu Wang
  • Ti Ding
  • Kai Yu
  • Philip R Taylor
  • Lemin Wang
  • Christian C Abnet
  • Hua Su
  • Nan Hu
  • Jin Hu Fan
  • Alisa M Goldstein
  • Neal D Freedman
  • Carol Giffen
  • You Lin Qiao
  • Thuyvi Luong
  • Laurie Burdett
  • Kathleen A Harrison
  • Jason Malphurs
  • Christopher M Cunniff
  • Michael L Cunningham
  • Jason M Johnson
  • Joshua D Smith
  • Deborah A Nickerson
  • Joel Abramowitz
  • Stanislas Lyonnet
  • Karsten Spicher
  • Andrew A Heggie
  • Sarah B Emery
  • R J Kimple
  • Muriel Holder
  • Timothy C Cox
  • S Mukhopadhyay
  • Michael J Bamshad
  • Bernd Nurnberg
  • Anne V Hing

Detail Information

Publications83

  1. ncbi Distinct guanine nucleotide binding and release properties of the three Gi proteins
    D J Carty
    Department of Pharmacology, Mount Sinai School of Medicine, City University, New York, New York 10029
    J Biol Chem 265:6268-73. 1990
    ....
  2. ncbi RGS10 is a selective activator of G alpha i GTPase activity
    T W Hunt
    Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts 02138, USA
    Nature 383:175-7. 1996
    ..These results demonstrate that RGS proteins can attenuate signalling pathways involving heterotrimeric G proteins by serving as GTPase-activating proteins for specific types of G alpha subunits...
  3. ncbi Structural basis for the selectivity of the RGS protein, GAIP, for Galphai family members. Identification of a single amino acid determinant for selective interaction of Galphai subunits with GAIP
    D S Woulfe
    Department of Pharmacology, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
    J Biol Chem 274:17718-24. 1999
    ..Thus, GAIP was able to discriminate between Galphai1 and Galphai2 in both binding and functional assays, and in both cases residue 229/230 played a critical role in selective recognition...
  4. ncbi Changes in expression of adenyl cyclase activity in human endometrium during hormone replacement therapy and ovarian stimulation
    L Bernardini
    Department of Obstetrics and Gynaecology, University of Genoa, Genoa, Italy
    Mol Hum Reprod 5:955-60. 1999
    ..These data strongly support the concept that the transmembrane signalling AC system and its messenger cyclic AMP are major regulators of endometrial function in the human...
  5. pmc Variations in Gnai2 and Rgs1 expression affect chemokine receptor signaling and the organization of secondary lymphoid organs
    I Y Hwang
    B Cell Molecular Immunology Section, Laboratory of Immunoregulation, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    Genes Immun 11:384-96. 2010
    ..Examination of B cells from mice with varying numbers of intact alleles of Ccr7, Rgs1, Gnai2, and Gnai3 provided the basis for these results...
  6. pmc GAIP, a protein that specifically interacts with the trimeric G protein G alpha i3, is a member of a protein family with a highly conserved core domain
    L de Vries
    Division of Cellular and Molecular Medicine, University of California, San Diego, La Jolla 92093 0651, USA
    Proc Natl Acad Sci U S A 92:11916-20. 1995
    ..The fact that GAIP and Sst2 interact with G alpha subunits and share a common domain suggests that other members of the GAIP family also interact with G alpha subunits through the 125-amino-acid core domain...
  7. pmc GAIP is membrane-anchored by palmitoylation and interacts with the activated (GTP-bound) form of G alpha i subunits
    L de Vries
    Division of Cellular and Molecular Medicine, University of California at San Diego, La Jolla 92093 0651, USA
    Proc Natl Acad Sci U S A 93:15203-8. 1996
    ..This motif, present in cysteine string proteins found on synaptic vesicles, pancreatic zymogen granules, and chromaffin granules, suggests GAIP's possible involvement in membrane trafficking...
  8. pmc Galpha i3 binding to calnuc on Golgi membranes in living cells monitored by fluorescence resonance energy transfer of green fluorescent protein fusion proteins
    T S Weiss
    Department of Cellular and Molecular Medicine, University of California at San Diego, La Jolla, CA 92093, USA
    Proc Natl Acad Sci U S A 98:14961-6. 2001
    ..Calnuc has the properties of a Ca(2+) sensor protein capable of binding to and potentially regulating interactions of Galphai3 on Golgi membranes...
  9. pmc Gialpha and Gbeta subunits both define selectivity of G protein activation by alpha2-adrenergic receptors
    Scott K Gibson
    Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA
    Proc Natl Acad Sci U S A 103:212-7. 2006
    ..The molecular properties of each Gialpha, Gbeta, and alpha2-adrenergic receptor subtype influence signaling efficiency for the alpha2-adrenergic receptor-mediated signaling pathway...
  10. ncbi Regional localization of the human G protein alpha i2 (GNAI2) gene: assignment to 3p21 and a related sequence (GNAI2L) to 12p12-p13
    I Magovcevic
    Department of Genetics, Brigham and Women s Hospital, Harvard Medical School, Boston, Massachusetts 02115
    Genomics 12:125-9. 1992
    ..family, include a small number of polypeptides: Gi alpha 1 (GNAI1), Gi alpha 2 (GNAI2), and Gi alpha 3 (GNAI3)...
  11. pmc Specific interaction of Gαi3 with the Oa1 G-protein coupled receptor controls the size and density of melanosomes in retinal pigment epithelium
    Alejandra Young
    Jules Stein Eye Institute, University of California Los Angeles, Los Angeles, California, United States of America
    PLoS ONE 6:e24376. 2011
    ..We had previously shown by in-vivo studies that Gαi3-/- and Oa1-/- mice have similar RPE phenotype and decussation patterns. In this paper we analyze the specificity of the Oa1-Gαi interaction...
  12. pmc A human homeotic transformation resulting from mutations in PLCB4 and GNAI3 causes auriculocondylar syndrome
    Mark J Rieder
    Department of Genome Sciences, University of Washington, Seattle, WA 98195, USA
    Am J Hum Genet 90:907-14. 2012
    ..two novel (exclusive to the patient and/or family studied) missense mutations in PLCB4 and a shared mutation in GNAI3 in two unrelated probands...
  13. pmc The loss of Gnai2 and Gnai3 in B cells eliminates B lymphocyte compartments and leads to a hyper-IgM like syndrome
    Il Young Hwang
    B Cell Molecular Immunology Section, Laboratory of Immunoregulation, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, United States of America
    PLoS ONE 8:e72596. 2013
    ..Gαi2 and Gαi3 are therefore critical for B cell chemoattractant receptor signaling and for normal B cell function. These mice provide a worst case scenario of the consequences of losing chemoattractant receptor signaling in B cells. ..
  14. doi Heterogeneity of mutational mechanisms and modes of inheritance in auriculocondylar syndrome
    Christopher T Gordon
    INSERM U781, Tour Lavoisier 2ème étage, Hopital Necker Enfants Malades, 149 rue de Sevres, Paris 75015, France
    J Med Genet 50:174-86. 2013
    ..C, β 4 (PLCB4) and guanine nucleotide binding protein (G protein), α inhibiting activity polypeptide 3 (GNAI3) genes have recently been identified in ACS patients by exome sequencing...
  15. pmc Activation state-dependent interaction between Galphai and p67phox
    Caroline Marty
    Department of Pharmacology, University of Illinois at Chicago, 835 S Wolcott Avenue, Chicago, IL 60612, USA
    Mol Cell Biol 26:5190-200. 2006
    ..These results provide the first evidence for an interaction between p67phox and an alpha subunit of heterotrimeric G proteins, suggesting a potential role for Galphai in the regulation or activation of NADPH oxidase...
  16. pmc Genetic variants in epidermal growth factor receptor pathway genes and risk of esophageal squamous cell carcinoma and gastric cancer in a Chinese population
    Wen Qing Li
    Division of Cancer Epidemiology and Genetics, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, USA
    PLoS ONE 8:e68999. 2013
    ..16×10(-3)). Gene-level analyses found 10 genes to be associated with GC, including FYN, MAPK8, MAP2K4, GNAI3, MAP2K1, TLN1, PRLR, PLCG2, RPS6KB2, and PIK3R3 (P<0.05)...
  17. pmc Development of the mammalian axial skeleton requires signaling through the Gα(i) subfamily of heterotrimeric G proteins
    Nicholas W Plummer
    Laboratory of Neurobiology, National Institute of Environmental Health Sciences, National Institutes of Health Department of Health and Human Services, Durham, NC 27709, USA
    Proc Natl Acad Sci U S A 109:21366-71. 2012
    129/SvEv mice with a loss-of-function mutation in the heterotrimeric G protein α-subunit gene Gnai3 have fusions of ribs and lumbar vertebrae, indicating a requirement for Gα(i) (the "inhibitory" class of α-subunits) in somite ..
  18. pmc PKA regulatory subunits mediate synergy among conserved G-protein-coupled receptor cascades
    Eduard Stefan
    Institute of Biochemistry and Center for Molecular Biosciences, University of Innsbruck, Innsbruck, Austria
    Nat Commun 2:598. 2011
    ..These findings suggest a direct mechanism by which coincident activation of Gαs-coupled receptors controls the precision of adaptive responses of activated Gαi-coupled receptor cascades...
  19. doi First detection of taste buds in a chimaeroid fish (Chondrichthyes: Holocephali) and their Gαi-like immunoreactivity
    Sara Ferrando
    DIPTERIS, University of Genoa, Viale Benedetto XV 5, I 16132 Genoa, Italy
    Neurosci Lett 517:98-101. 2012
    ..fish with a sequenced genome; the highlighted sequences could suggest the presence of all three genes (gnai1, gnai2 and gnai3) in holocephalans. The sequences of the predicted proteins present a high identity with the mammalian proteins.
  20. pmc Subcellular localization of LGN during mitosis: evidence for its cortical localization in mitotic cell culture systems and its requirement for normal cell cycle progression
    Rachna Kaushik
    Institute of Molecular and Cell Biology, Singapore 117609
    Mol Biol Cell 14:3144-55. 2003
    ..The results also show that the cortical localization of LGN is dependent on microfilaments and that interfering with LGN function in cultured cell lines causes early disruption to cell cycle progression...
  21. ncbi Mammalian Ric-8A (synembryn) is a heterotrimeric Galpha protein guanine nucleotide exchange factor
    Gregory G Tall
    Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas 75390 9041, USA
    J Biol Chem 278:8356-62. 2003
    ..Ric-8A interacts with GDP-bound Galpha proteins, stimulates release of GDP, and forms a stable nucleotide-free transition state complex with the Galpha protein; this complex dissociates upon binding of GTP to Galpha...
  22. ncbi Regulator of G protein signaling Z1 (RGSZ1) interacts with Galpha i subunits and regulates Galpha i-mediated cell signaling
    Yuren Wang
    Neuroscience Discovery Research, Wyeth Research, Princeton, New Jersey 08543 8000, USA
    J Biol Chem 277:48325-32. 2002
    ..In summary, these data suggest that RGSZ1 serves as a GTPase accelerating protein for Galpha(i) and regulates Galpha(i)-mediated signaling, thus expanding the potential role of RGSZ1 in G protein-mediated cellular activities...
  23. ncbi Oxidized human neuroglobin acts as a heterotrimeric Galpha protein guanine nucleotide dissociation inhibitor
    Keisuke Wakasugi
    Department of Molecular Engineering, Graduate School of Engineering, Kyoto University, Japan
    J Biol Chem 278:36505-12. 2003
    ..In contrast, ferrous ligand-bound Ngb under normoxia did not have GDI activities. Taken together, we propose that human Ngb may be a novel oxidative stress-responsive sensor for signal transduction in the brain...
  24. ncbi P2Y(13): identification and characterization of a novel Galphai-coupled ADP receptor from human and mouse
    Fang L Zhang
    Human Genome Research, Schering Plough Research Institute, Kenilworth, New Jersey 07033, USA
    J Pharmacol Exp Ther 301:705-13. 2002
    ..In keeping with the general nomenclature for the purinergic receptors, we propose designating this novel receptor P2Y(13)...
  25. ncbi Cloning and characterization of a novel regulator of G protein signalling in human platelets
    Alison W Gagnon
    Cardiovascular Drug Discovery, Aventis Pharmaceuticals, 500 Arcola Road, Collegeville, PA 19426, USA
    Cell Signal 14:595-606. 2002
    ..RGS18 is expressed in platelet, leukocyte, and megakaryocyte cell lines and binds to endogenous Galphai1, Galphai2, Galphai3, and Galphaq but not Galphaz, Galphas or Galpha12 in vitro...
  26. pmc Promotion of G alpha i3 subunit down-regulation by GIPN, a putative E3 ubiquitin ligase that interacts with RGS-GAIP
    Thierry Fischer
    Department of Cellular and Molecular Medicine, University of California at San Diego, La Jolla, CA 92093, USA
    Proc Natl Acad Sci U S A 100:8270-5. 2003
    ..RGS-GAIP functions as a bifunctional adaptor that binds to G alpha subunits through its RGS domain and to GIPN through its cysteine string motif...
  27. pmc RGS18 is a myeloerythroid lineage-specific regulator of G-protein-signalling molecule highly expressed in megakaryocytes
    D Yowe
    Millennium Pharmaceuticals, 75 Sidney Street, Cambridge, MA 02139, USA
    Biochem J 359:109-18. 2001
    ..Hence RGS18 might have an important role in the regulation of megakaryocyte differentiation and chemotaxis...
  28. ncbi Phosphorylation and nuclear translocation of a regulator of G protein signaling (RGS10)
    P G Burgon
    Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts 02138, USA
    J Biol Chem 276:32828-34. 2001
    ..Rather, the phosphorylation event correlates with translocation of RGS10 from the plasma membrane and cytosol into the nucleus...
  29. ncbi RGS12 and RGS14 GoLoco motifs are G alpha(i) interaction sites with guanine nucleotide dissociation inhibitor Activity
    R J Kimple
    Department of Pharmacology, Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill, North Carolina 27599 7365, USA
    J Biol Chem 276:29275-81. 2001
    ..The presence of two, independent G alpha interaction sites suggests that RGS12 and RGS14 participate in a complex coordination of G-protein signaling beyond simple G alpha GAP activity...
  30. ncbi Characterization of RGS5 in regulation of G protein-coupled receptor signaling
    J Zhou
    Department of Biochemistry and Molecular Pharmacology, Chiba University Graduate School of Medicine, Japan
    Life Sci 68:1457-69. 2001
    ....
  31. ncbi Identification of the platelet ADP receptor targeted by antithrombotic drugs
    G Hollopeter
    Department of Cellular and Molecular Pharmacology, University of California, San Francisco 94143, USA
    Nature 409:202-7. 2001
    ..Cloning of the P2Y12 receptor should facilitate the development of better antiplatelet agents to treat cardiovascular diseases...
  32. ncbi Analysis of RGSZ1 protein interaction with Galphai subunits
    Yuren Wang
    Neuroscience Discovery Research, Wyeth Research, Princeton, New Jersey 08543, USA
    Methods Enzymol 390:31-52. 2004
    ....
  33. ncbi Severe autosomal recessive retinitis pigmentosa maps to chromosome 1p13.3-p21.2 between D1S2896 and D1S457 but outside ABCA4
    Qingjiong Zhang
    Ophthalmic Genetics and Visual Function Branch, National Eye Institute, National Institutes of Health, Building 10, Room 10B10, 10 Center Drive, MSC 1860, Bethesda, MD, 20892 1860, USA
    Hum Genet 118:356-65. 2005
    ..Sequencing the exons and intron-exon boundaries of five candidate genes and six ESTs in this region, OLFM3, GNAI3, LOC126987, FLJ25070, DKFZp586G0123, AV729694, BU662869, BU656110, BU171991, BQ953690, and CA397743, did not ..
  34. pmc Two distinct aspects of coupling between Gα(i) protein and G protein-activated K+ channel (GIRK) revealed by fluorescently labeled Gα(i3) protein subunits
    Shai Berlin
    Department of Physiology and Pharmacology, Sackler School of Medicine, Tel Aviv University, Tel Aviv 69978, Israel
    J Biol Chem 286:33223-35. 2011
    ..Our findings support the notion that Gα(i/o) actively regulates GIRK. Although regulation of I(basal) is a function of Gα(i)(GDP), our new findings indicate that regulation of kinetics of I(evoked) is mediated by Gα(i)(GTP)...
  35. pmc A GDI (AGS3) and a GEF (GIV) regulate autophagy by balancing G protein activity and growth factor signals
    Mikel Garcia-Marcos
    Departments of Cellular and Molecular Medicine, University of California, San Diego, La Jolla, CA 92093, USA
    Mol Biol Cell 22:673-86. 2011
    ..These results provide mechanistic insights into how reversible modulation of Gα(i3) activity by AGS3 and GIV maintains the delicate equilibrium between promotion and inhibition of autophagy...
  36. doi TNFAIP8: a new effector for Galpha(i) coupling to reduce cell death and induce cell transformation
    Benoit Laliberte
    Ottawa Hospital Research Institute Neuroscience, University of Ottawa, Ottawa, ON, Canada
    J Cell Physiol 225:865-74. 2010
    ..The data suggest that Gαi-TNFAIP8-mediated rescue of pre-oncogenic cells enhances progression to oncogenic transformation, providing a selective target to inhibit cellular transformation...
  37. doi Activation of mast cells by trimeric G protein Gi3; coupling to the A3 adenosine receptor directly and upon T cell contact
    Dana Baram
    Department of Cell and Developmental Biology, Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel
    J Immunol 184:3677-88. 2010
    ..These results unveil the physiological G-protein coupled receptor that couples to Gi3 and establish the important role played by this G-protein in inflammatory conditions that involve adenosine-activated mast cells...
  38. doi Genome-wide association study of recurrent early-onset major depressive disorder
    J Shi
    Department of Psychiatry, Stanford University, Stanford, CA, USA
    Mol Psychiatry 16:193-201. 2011
    ..Larger samples will be required to confirm the hypothesis of association between MDD (and particularly the recurrent early-onset subtype) and common SNPs...
  39. pmc Insulin-like growth factor-binding protein-5 stimulates growth of human intestinal muscle cells by activation of G{alpha}i3
    Robert S Flynn
    Department of Medicine, Virginia Commonwealth University, Richmond, USA
    Am J Physiol Gastrointest Liver Physiol 297:G1232-8. 2009
    ..Our results indicate that endogenous IGFBP-5 activates Gi3 and regulates smooth muscle growth, IGF-I production, and collagen production via the alpha-subunit of Gi3, independently of IGF-I, in normal human intestinal muscle cells...
  40. pmc Neither replication nor simulation supports a role for the axon guidance pathway in the genetics of Parkinson's disease
    Yonghong Li
    Celera, Alameda, California, United States of America
    PLoS ONE 3:e2707. 2008
    ..Together, these results challenge the robustness of the reported panel of genetic markers to predict PD risk in particular and a role of the axon guidance pathway in PD genetics in general...
  41. ncbi Expression, purification, and preliminary X-ray crystallographic analysis of the complex of G(alphai3)-RGS5 from human with GDP/Mg2+)/AlF4-
    Kyung Hee Rhee
    Life Sciences Division, Biomedical Research Center, Korea Institute of Science and Technology, Division of Biotechnology, Korea University, Seoul 136 701, South Korea
    Protein Pept Lett 13:945-9. 2006
    ..9 A, and c=138.8 A. Assuming one complex protein in the crystallographic asymmetric unit, the calculated Matthews parameter (V(M)) is 2.57 A(3)/Da and solvent content is 52.2 %...
  42. pmc Distribution of activator of G-protein signaling 3 within the aggresomal pathway: role of specific residues in the tetratricopeptide repeat domain and differential regulation by the AGS3 binding partners Gi(alpha) and mammalian inscuteable
    Ali Vural
    Department of Cell and Molecular Pharmacology and Experimental Therapeutics, Medical University of South Carolina, Charleston, South Carolina 29425, USA
    Mol Cell Biol 30:1528-40. 2010
    ..These data present AGS3, G-proteins, and mInsc as candidate proteins involved in regulating cellular stress associated with protein-processing pathologies...
  43. pmc Crystallization and preliminary X-ray crystallographic analysis of human RGS10 complexed with Galphai3
    Hyung Ki Lee
    Life Sciences Division, Korea Institute of Science and Technology, Seoul 130 650, South Korea
    Acta Crystallogr Sect F Struct Biol Cryst Commun 61:831-3. 2005
    ..88, b = 99.88, c = 144.59 A, alpha = beta = gamma = 90 degrees . A full set of diffraction data were collected to 2.5 A resolution at 100 K using synchrotron radiation at Pohang beamline 4A...
  44. ncbi G-protein alpha subunit interaction and guanine nucleotide dissociation inhibitor activity of the dual GoLoco motif protein PCP-2 (Purkinje cell protein-2)
    Francis S Willard
    Department of Pharmacology, CB 7365, 1106 Mary Ellen Jones Building, University of North Carolina, Chapel Hill, NC 27599 7365, USA
    Cell Signal 18:1226-34. 2006
    ..Collectively, the data demonstrate that PCP-2 is a comparatively weak GoLoco motif protein that exhibits highest affinity interactions and GDI activity toward Galphai1, Galphai2, and Galphai3 subunits...
  45. ncbi Mechanism of the receptor-catalyzed activation of heterotrimeric G proteins
    William M Oldham
    Department of Pharmacology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232 6600, USA
    Nat Struct Mol Biol 13:772-7. 2006
    ..These data provide the first direct observation of R*-mediated conformational changes in G proteins and define the structural basis for GDP release from G alpha...
  46. ncbi CB1 receptor-G protein association. Subtype selectivity is determined by distinct intracellular domains
    S Mukhopadhyay
    Department of Pharmacological and Physiological Science, Saint Louis University School of Medicine, St Louis, MO, USA
    Eur J Biochem 268:499-505. 2001
    ..These studies indicate that different domains of the CB(1) receptor direct the interaction with specific G protein subtypes...
  47. pmc Guanine nucleotide-binding regulatory proteins in retinal pigment epithelial cells
    M Jiang
    Department of Microbiology, University of Southern California School of Medicine, Los Angeles 90033
    Proc Natl Acad Sci U S A 88:3907-11. 1991
    ..The results indicate that this G alpha subunit belongs to a separate subfamily of G proteins that may be insensitive to inhibition by pertussis toxin...
  48. ncbi The AT2 receptor selectively associates with Gialpha2 and Gialpha3 in the rat fetus
    J Zhang
    Division of Cardiovascular Medicine, Falk Cardiovascular Research Center, Stanford University School of Medicine, Stanford, California 94305 5246, USA
    J Biol Chem 271:15026-33. 1996
    ..The finding that AT2 receptor couples to both Gialpha2 and Gialpha3 raises the possibility that selective interactions between AT2 receptor and different G proteins may result in specific cellular effects mediated by AT2 stimulation...
  49. pmc The association between glycosylphosphatidylinositol-anchored proteins and heterotrimeric G protein alpha subunits in lymphocytes
    K R Solomon
    Laboratory of Infectious Diseases, Dana Farber Cancer Institute, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 93:6053-8. 1996
    ..These experiments suggest a potential role of G proteins in the transduction of signals generated by GPI-anchored molecules expressed on lymphocytes of both mouse and human...
  50. ncbi The inducible G protein-coupled receptor edg-1 signals via the G(i)/mitogen-activated protein kinase pathway
    M J Lee
    Department of Molecular Biology, Holland Laboratory, American Red Cross, Rockville, Maryland 20855, USA
    J Biol Chem 271:11272-9. 1996
    ..These data suggest that the G(i)/mitogen-activated protein kinase pathway is a major signaling pathway regulated by the orphan receptor edg-1...
  51. pmc The human thyrotropin receptor: a heptahelical receptor capable of stimulating members of all four G protein families
    K L Laugwitz
    Institut fur Pharmakologie, Freie Universitat Berlin, Germany
    Proc Natl Acad Sci U S A 93:116-20. 1996
    ....
  52. ncbi The structure of the G protein heterotrimer Gi alpha 1 beta 1 gamma 2
    M A Wall
    Department of Biochemistry, University of Texas Southwestern Medical Center, Dallas 75235, USA
    Cell 83:1047-58. 1995
    ..Repeated WD motifs in beta form a circularized sevenfold beta propeller. The conserved cores of these motifs are a scaffold for display of their more variable linkers on the exterior face of each propeller blade...
  53. ncbi Identification and expression of G-proteins in human myometrium: up-regulation of G alpha s in pregnancy
    G N Europe-Finner
    University of Oxford, Nuffield Department of Obstetrics and Gynecology, John Radcliffe Hospital, Headington, United Kingdom
    Endocrinology 132:2484-90. 1993
    ..The increase in G alpha s levels may play a crucial role in maintaining relaxation of the uterus by favoring cAMP formation during pregnancy...
  54. ncbi G protein-coupled signal transduction pathways for interleukin-8
    D Wu
    Division of Biology, California Institute of Technology, Pasadena 91125
    Science 261:101-3. 1993
    ..These findings suggest that IL-8 acts through signal-transducing pathways that are limited to specific heterotrimeric G proteins and effectors. These may provide suitable targets for the development of anti-inflammatory agents...
  55. ncbi Cell-specific physical and functional coupling of human 5-HT1A receptors to inhibitory G protein alpha-subunits and lack of coupling to Gs alpha
    J R Raymond
    Department of Medicine, Duke University Medical Center, Durham, North Carolina 27710
    Biochemistry 32:11064-73. 1993
    ..One factor influencing the relative importance of those G proteins for 5-HT1A receptor-inhibited adenylylcyclase activity appears to be their-relative levels of expression.(ABSTRACT TRUNCATED AT 250 WORDS)..
  56. ncbi Structural determinants for activation of the alpha-subunit of a heterotrimeric G protein
    D G Lambright
    Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut 06510
    Nature 369:621-8. 1994
    ..The changes are distinct from those observed in other members of the GTPase superfamily...
  57. ncbi Stabilization of C5a receptor--G-protein interactions through ligand binding
    L P Wennogle
    Research Department, Ciba Geigy Pharmaceuticals Division, Summit, New Jersey 07901
    J Cell Biochem 55:380-8. 1994
    ..The method of stabilization of receptor-transducer complexes offers the opportunity to further elaborate the interactions of the C5a receptor with diverse transducing elements and second messenger systems...
  58. ncbi Differential coupling of m2 and m4 muscarinic receptors to inhibition of adenylyl cyclase by Gi alpha and G(o)alpha subunits
    J C Migeon
    Department of Pharmacology, University of Washington, Seattle 98195 7750, USA
    J Biol Chem 270:16070-4. 1995
    ..The m2 and m4 mAChRs thus couple to inhibition of adenylyl cyclase by overlapping but different sets of G-protein alpha subunits...
  59. pmc Identification of cDNA encoding an additional alpha subunit of a human GTP-binding protein: expression of three alpha i subtypes in human tissues and cell lines
    S Y Kim
    Department of Medicine, Brigham and Women s Hospital, Boston, MA 02115
    Proc Natl Acad Sci U S A 85:4153-7. 1988
    ..Thus, some cells contain all three alpha i subtypes. This observation raises the question of how cells prevent cross talk among receptors that are coupled to effectors through such similar alpha proteins...
  60. pmc A small multigene family encodes Gi signal-transduction proteins
    C R Beals
    Howard Hughes Medical Institute, University of Washington, Seattle 98195
    Proc Natl Acad Sci U S A 84:7886-90. 1987
    ..Our data support the view that a modest repertoire of extremely closely related G proteins mediates the transduction of signals derived from multiple different receptor molecules...
  61. ncbi Molecular cloning of a new human G protein. Evidence for two Gi alpha-like protein families
    J R Didsbury
    FEBS Lett 219:259-63. 1987
    ..One group probably contains true Gi and the other a new class of G protein whose function remains to be determined...
  62. pmc Chromosomal localization of genes encoding guanine nucleotide-binding protein subunits in mouse and human
    C Blatt
    Weizmann Institute, Rehovoth, Israel
    Proc Natl Acad Sci U S A 85:7642-6. 1988
    ..The retinal-specific transducin subunit genes GNAT1 and GNAT2 were on chromosomes 3 and 1; GNAI1, GNAI2, and GNAI3 were assigned to chromosomes 7, 3, and 1, respectively; GNAZ and GNAS were found on chromosomes 22 and 20...
  63. ncbi Alpha i-3 cDNA encodes the alpha subunit of Gk, the stimulatory G protein of receptor-regulated K+ channels
    J Codina
    Department of Cell Biology, Baylor College of Medicine, Houston, Texas 77030
    J Biol Chem 263:6746-50. 1988
    ..The probable identity of alpha i-1 with alpha p and possible roles for alpha i-2, as well as additional roles for alpha i-1 and alpha i-3 (alpha k) are discussed...
  64. ncbi Cloning and characterization of the human phosphoinositide-specific phospholipase C-beta 1 (PLC beta 1)
    A Caricasole
    Biology Department, GlaxoWellcome Medicines Research Centre, Verona, Italy
    Biochim Biophys Acta 1517:63-72. 2000
    ..Finally, we characterized the structure of the PLC beta 1 locus and confirmed its mapping to human chromosome 20...
  65. ncbi The G protein subunit gene families
    G B Downes
    Department of Anesthesiology, Washington University School of Medicine, St Louis, Missouri 63110, USA
    Genomics 62:544-52. 1999
  66. ncbi Dual signaling of human Mel1a melatonin receptors via G(i2), G(i3), and G(q/11) proteins
    L Brydon
    CNRS UPR 0415 and Université Paris VII, Institut Cochin de Genetique Moleculaire, Paris, France
    Mol Endocrinol 13:2025-38. 1999
    ..We show that Mel 1a receptors functionally couple to both PTX-sensitive and PTX-insensitive G proteins. The previously unknown signaling of Mel 1a receptors through G(q/11) widens the spectrum of potential targets for melatonin...
  67. ncbi Functional reconstitution of the angiotensin II type 2 receptor and G(i) activation
    J L Hansen
    Laboratory for Molecular Cardiology and the Department of Medicine B, University of Copenhagen, Denmark
    Circ Res 87:753-9. 2000
    ..In combination with previous observations, these results show that the AT(2) receptor is fully capable of activating G(i) and provides a new tool for exploring AT(2) receptor pharmacology and interactions with G-protein trimers...
  68. pmc Initiation of DNA replication at the Chinese hamster origin oriGNAI3 relies on local sequences and/or chromatin structures, but not on transcription of the nearby GNAI3 gene
    F Toledo
    Unité de Génétique Somatique URA CNRS 1960, Institut Pasteur, 25 rue du Dr Roux, 75724 Paris Cedex 15, France
    Nucleic Acids Res 27:1600-8. 1999
    ..identified a region of preferential replication initiation, oriGNAI3, near the 3' end of the Chinese hamster GNAI3 gene...
  69. ncbi Regulators of G protein signaling exhibit distinct patterns of gene expression and target G protein specificity in human lymphocytes
    C Beadling
    Immunology Program, Cornell University Graduate School of Medical Sciences, Division of Immunology, Cornell University Medical College, New York, NY 10021, USA
    J Immunol 162:2677-82. 1999
    ....
  70. pmc RGS3 inhibits G protein-mediated signaling via translocation to the membrane and binding to Galpha11
    N O Dulin
    Department of Medicine and Cardiovascular Research Center, Medical College of Wisconsin, Milwaukee, Wisconsin 53226, USA
    Mol Cell Biol 19:714-23. 1999
    ..These data indicate that RGS3 inhibits G protein-coupled receptor signaling by a complex mechanism involving its translocation to the membrane in addition to its established function as a GTPase-activating protein...
  71. pmc A single receptor encoded by vzg-1/lpA1/edg-2 couples to G proteins and mediates multiple cellular responses to lysophosphatidic acid
    N Fukushima
    Department of Pharmacology, School of Medicine, University of California at San Diego, La Jolla, CA 92093 0636, USA
    Proc Natl Acad Sci U S A 95:6151-6. 1998
    ..These results demonstrate that a single receptor, encoded by vzg-1, can activate multiple LPA-dependent responses in cells from distinct tissue lineages...
  72. pmc oriGNAI3: a narrow zone of preferential replication initiation in mammalian cells identified by 2D gel and competitive PCR replicon mapping techniques
    F Toledo
    Unité de Génétique Somatique URA CNRS 1960, Institut Pasteur, 25 rue du Dr Roux, 75724 Paris Cedex 15, France
    Nucleic Acids Res 26:2313-21. 1998
    ..In this report we analyze the replication of an amplified genomic region encompassing the 3'-end of the GNAI3 gene, the entire GNAT2 gene and the intergenic region between them in exponentially growing Chinese hamster ..
  73. ncbi Characterization of a novel mammalian RGS protein that binds to Galpha proteins and inhibits pheromone signaling in yeast
    C Chen
    Regulatory Biology Laboratory, Institute of Molecular and Cell Biology, National University of Singapore, 10 Kent Ridge Crescent, Singapore 119260, Republic of Singapore
    J Biol Chem 272:8679-85. 1997
    ....
  74. ncbi Coupling of dopamine receptor subtypes to multiple and diverse G proteins
    A Sidhu
    Laboratory of Molecular Neurochemistry, Department of Pediatrics, Georgetown University Medical Center, Georgetown University, 3970 Reservoir Road, NW, Washington, DC 20007, USA
    Int J Dev Neurosci 18:669-77. 2000
    ....
  75. pmc The mammalian calcium-binding protein, nucleobindin (CALNUC), is a Golgi resident protein
    P Lin
    Division of Cellular and Molecular Medicine and Department of Pathology, University of California, San Diego, La Jolla, California 92093 0651, USA
    J Cell Biol 141:1515-27. 1998
    ..The properties of CALNUC and its high homology to calreticulin suggest that it may play a key role in calcium homeostasis in the CGN and cis-Golgi cisternae...
  76. doi Dissociation of heterotrimeric g proteins in cells
    Nevin A Lambert
    Department of Pharmacology and Toxicology, Medical College of Georgia, Augusta, GA 30912 2300, USA
    Sci Signal 1:re5. 2008
    ..This unexpectedly reluctant dissociation calls for inclusion of a GTP-bound heterotrimeric state in models of the G protein cycle and places renewed emphasis on the relation between subunit dissociation and effector activation...
  77. ncbi Presence of three distinct molecular species of Gi protein alpha subunit. Structure of rat cDNAs and human genomic DNAs
    H Itoh
    Institute of Medical Science, University of Tokyo, Japan
    J Biol Chem 263:6656-64. 1988
    ..Southern blot analysis indicates that a single copy of each Gi alpha gene is present per haploid human genome...
  78. ncbi The human genome encodes at least three non-allellic G proteins with alpha i-type subunits
    W N Suki
    FEBS Lett 220:187-92. 1987
    ....
  79. ncbi Interaction of folate receptor with signaling molecules lyn and G(alpha)(i-3) in detergent-resistant complexes from the ovary carcinoma cell line IGROV1
    S Miotti
    Unit of Molecular Therapies, Department of Experimental Oncology, Istituto Nazionale per lo Studio e la Cura dei Tumori, Milan, Italy
    J Cell Sci 113:349-57. 2000
    ..These findings point to the functional interaction between folate receptor and associated signaling molecules...
  80. ncbi RGS7 and RGS8 differentially accelerate G protein-mediated modulation of K+ currents
    O Saitoh
    Department of Molecular and Cellular Neurobiology, Tokyo Metropolitan Institute for Neuroscience, 2 6 Musashidai, Fuchu shi, Tokyo 183 8526, Japan
    J Biol Chem 274:9899-904. 1999
    ..These acceleration properties of RGS proteins may play important roles in the rapid regulation of neuronal excitability and the cellular responses to short-lived stimulations...
  81. pmc Activation of Galphai3 triggers cell migration via regulation of GIV
    Pradipta Ghosh
    Department of Cellular and Molecular Medicine, University of California, San Diego, La Jolla, CA 92093, USA
    J Cell Biol 182:381-93. 2008
    ....
  82. ncbi CCL3, acting via the chemokine receptor CCR5, leads to independent activation of Janus kinase 2 (JAK2) and Gi proteins
    Anja Mueller
    School of Animal and Microbial Sciences, University of Reading, Whiteknights, Reading RG6 6AJ, UK
    FEBS Lett 570:126-32. 2004
    ..Gi protein activation was not prevented by inhibition of JAK, showing that heterotrimeric G protein activation and activation of the JAK/signal transducer and activator of transcription (STAT) pathway are independent of each other...
  83. ncbi Leukotriene D4-induced activation and translocation of the G-protein alpha i3-subunit in human epithelial cells
    J L Adolfsson
    Division of Experimental Pathology, Lund University, Wallenberg Laboratory, Malmo, Sweden
    Biochem Biophys Res Commun 226:413-9. 1996
    ..Furthermore, the translocation of G alpha i3-subunits to the cytoskeleton and the simultaneous inhibition of G3-proteins indicate that the cytoskeleton might participate in the signalling process of human epithelial cells...

Research Grants17

  1. Structure and mechanism of beta2-adrenergic receptor
    Roger Sunahara; Fiscal Year: 2007
    ..Finally, I will integrate these data to formulate a model of how hormone activation of beta2R leads to activation of nucleotide exchange and propose that this model will extend to the entire family of GPCRs. ..
  2. GPCR signaling complexes in living cells
    Nevin Lambert; Fiscal Year: 2009
    ..g. when and where receptors, G-proteins and effector molecules interact with each other to transmit signals. The goal of this project is to provide this information. ..
  3. GPCR signaling complexes in living cells
    NEVIN ALAN LAMBERT; Fiscal Year: 2010
    ..g. when and where receptors, G-proteins and effector molecules interact with each other to transmit signals. The goal of this project is to provide this information. ..
  4. GPCR signaling complexes in living cells
    Nevin Lambert; Fiscal Year: 2009
    ..g. when and where receptors, G-proteins and effector molecules interact with each other to transmit signals. The goal of this project is to provide this information. ..
  5. Regulation of G-protein signaling in CNS neurons
    Nevin Lambert; Fiscal Year: 2006
    ..If our hypothesis is correct, we will identify presynaptic terminals as key sites for the adaptive changes responsible for the development of drug effects, drug tolerance and drug dependence. ..
  6. CALCIUM AND ENDOCYTOTIC MEMBRANE RETRIEVAL
    Nevin Lambert; Fiscal Year: 2003
    ..Specifically we will determine how exocytotic activity influences membrane depolarization and the cellular distribution of P-type calcium channels. ..
  7. GPCR signaling complexes in living cells
    NEVIN ALAN LAMBERT; Fiscal Year: 2010
    ..g. when and where receptors, G-proteins and effector molecules interact with each other to transmit signals. The goal of this project is to provide this information. ..