GNAI1

Summary

Gene Symbol: GNAI1
Description: G protein subunit alpha i1
Alias: guanine nucleotide-binding protein G(i) subunit alpha-1, Gi1 protein alpha subunit, adenylate cyclase-inhibiting G alpha protein, guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 1
Species: human
Products:     GNAI1

Top Publications

  1. Du Q, Macara I. Mammalian Pins is a conformational switch that links NuMA to heterotrimeric G proteins. Cell. 2004;119:503-16 pubmed
    ..We propose that a related switch mechanism might operate in asymmetric cell divisions in the fly and nematode. ..
  2. Gibson S, Gilman A. Gialpha and Gbeta subunits both define selectivity of G protein activation by alpha2-adrenergic receptors. Proc Natl Acad Sci U S A. 2006;103:212-7 pubmed
    ..The molecular properties of each Gialpha, Gbeta, and alpha2-adrenergic receptor subtype influence signaling efficiency for the alpha2-adrenergic receptor-mediated signaling pathway. ..
  3. Kiyomitsu T, Cheeseman I. Chromosome- and spindle-pole-derived signals generate an intrinsic code for spindle position and orientation. Nat Cell Biol. 2012;14:311-7 pubmed publisher
    ..We propose that these chromosome- and spindle-pole-derived gradients generate an intrinsic code to control spindle position and orientation. ..
  4. Oikawa H, Goh W, Lim V, Wong L, Sng J. Valproic acid mediates miR-124 to down-regulate a novel protein target, GNAI1. Neurochem Int. 2015;91:62-71 pubmed publisher
    ..and iTRAQ data that miR-124 is likely to target at guanine nucleotide binding protein alpha inhibitor 1 (GNAI1), an adenylate cyclase inhibitor...
  5. Li Z, Sun B, Gong T, Guo S, Zhang J, Wang J, et al. GNAI1 and GNAI3 Reduce Colitis-Associated Tumorigenesis in Mice by Blocking IL6 Signaling and Downregulating Expression of GNAI2. Gastroenterology. 2019;: pubmed publisher
    ..We investigated these signaling pathways and the involvement of G protein subunit alpha i1 (GNAI1), GNAI2, and GNAI3 in the development of CAC in mice and humans...
  6. Briscoe C, Tadayyon M, Andrews J, Benson W, Chambers J, Eilert M, et al. The orphan G protein-coupled receptor GPR40 is activated by medium and long chain fatty acids. J Biol Chem. 2003;278:11303-11 pubmed
    ..These data suggest that some of the physiological effects of fatty acids in pancreatic islets and brain may be mediated through a cell-surface receptor. ..
  7. Lyssand J, Bajjalieh S. The heterotrimeric [corrected] G protein subunit G alpha i is present on mitochondria. FEBS Lett. 2007;581:5765-8 pubmed
    ..This finding suggests that G protein receptor signaling may be a feature common to all membranes. ..
  8. Agrawal J, Ludwig B, Roy B, Dwivedi Y. Chronic Testosterone Increases Impulsivity and Influences the Transcriptional Activity of the Alpha-2A Adrenergic Receptor Signaling Pathway in Rat Brain. Mol Neurobiol. 2018;: pubmed publisher
    ..receptor (Adra2a), G proteins (stimulatory subunit-Gαs [Gnas], inhibitory subunit-G [Gnai1 and Gnai2]), and catalytic and regulatory subunits of protein kinase A (PKA) were examined using quantitative PCR (..
  9. Levoye A, Dam J, Ayoub M, Guillaume J, Couturier C, Delagrange P, et al. The orphan GPR50 receptor specifically inhibits MT1 melatonin receptor function through heterodimerization. EMBO J. 2006;25:3012-23 pubmed
    ..Pairing orphan GPCRs to potential heterodimerization partners might be of clinical importance and may become a general strategy to better understand the function of orphan GPCRs. ..

More Information

Publications142 found, 100 shown here

  1. Vila Coro A, Rodriguez Frade J, Martín de Ana A, Moreno Ortíz M, Martinez A C, Mellado M. The chemokine SDF-1alpha triggers CXCR4 receptor dimerization and activates the JAK/STAT pathway. FASEB J. 1999;13:1699-710 pubmed
    ..We present compelling evidence that the chemokines signal through mechanisms similar to those activated by cytokines. ..
  2. Hallak H, Seiler A, Green J, Ross B, Rubin R. Association of heterotrimeric G(i) with the insulin-like growth factor-I receptor. Release of G(betagamma) subunits upon receptor activation. J Biol Chem. 2000;275:2255-8 pubmed
    ..These results demonstrate an association of heterotrimeric G(i) with the IGF-IR and identify a discrete pool of G(betagamma) subunits available for downstream signaling following stimulation with IGF-I. ..
  3. Li C, Tian M, Zhang L, Fu Q, Song L, Yang N. Expression profiling and functional characterization of CD36 in turbot (Scophthalmus maximus L.). Fish Shellfish Immunol. 2018;81:485-492 pubmed publisher
    ..The syntenic analysis revealed that CD36 located between GNAI1 and SEMA3C genes across all the selected species, which suggested the synteny encompassing CD36 region during ..
  4. Nagata K, Katada T, Tohkin M, Itoh H, Kaziro Y, Ui M, et al. GTP-binding proteins in human platelet membranes serving as the specific substrate of islet-activating protein, pertussis toxin. FEBS Lett. 1988;237:113-7 pubmed
    ..These results indicate that the major IAP substrate present in human platelet membranes is a product of the Gi 2 alpha gene. ..
  5. Kagimoto S, Yamada Y, Kubota A, Someya Y, Ihara Y, Yasuda K, et al. Human somatostatin receptor, SSTR2, is coupled to adenylyl cyclase in the presence of Gi alpha 1 protein. Biochem Biophys Res Commun. 1994;202:1188-95 pubmed
    ..These results indicate that human SSTR2 is functionally coupled to Gi alpha 1 protein but not to Gi alpha 2 or Gi alpha 3 when expressed in CHO cells. ..
  6. Kleuss C, Raw A, Lee E, Sprang S, Gilman A. Mechanism of GTP hydrolysis by G-protein alpha subunits. Proc Natl Acad Sci U S A. 1994;91:9828-31 pubmed
  7. Wall M, Coleman D, Lee E, Iñiguez Lluhí J, Posner B, Gilman A, et al. The structure of the G protein heterotrimer Gi alpha 1 beta 1 gamma 2. Cell. 1995;83:1047-58 pubmed
    ..Repeated WD motifs in beta form a circularized sevenfold beta propeller. The conserved cores of these motifs are a scaffold for display of their more variable linkers on the exterior face of each propeller blade. ..
  8. Popov S, Yu K, Kozasa T, Wilkie T. The regulators of G protein signaling (RGS) domains of RGS4, RGS10, and GAIP retain GTPase activating protein activity in vitro. Proc Natl Acad Sci U S A. 1997;94:7216-20 pubmed
    ..Comparable protein-protein interactions between Gialpha1-GDP-AlF4- and the RGS domain or full-length RGS4 were detected using surface plasmon resonance. ..
  9. Raymond J, Mukhin Y, Gelasco A, Turner J, Collinsworth G, Gettys T, et al. Multiplicity of mechanisms of serotonin receptor signal transduction. Pharmacol Ther. 2001;92:179-212 pubmed
    ..This review will focus on what is known about the signaling linkages of the G-protein-linked 5-HT receptors, and will highlight some fascinating new insights into 5-HT receptor signaling. ..
  10. Gräler M, Grosse R, Kusch A, Kremmer E, Gudermann T, Lipp M. The sphingosine 1-phosphate receptor S1P4 regulates cell shape and motility via coupling to Gi and G12/13. J Cell Biochem. 2003;89:507-19 pubmed
    ..The capacity of S1P(4) to mediate cellular responses, such as motility and shape change through Galpha(i)- and Galpha(12/13)-coupled signaling pathways may be important for its in vivo function which is currently under investigation. ..
  11. Milde M, Rinne A, Wunder F, Engelhardt S, Bünemann M. Dynamics of G?i1 interaction with type 5 adenylate cyclase reveal the molecular basis for high sensitivity of Gi-mediated inhibition of cAMP production. Biochem J. 2013;454:515-23 pubmed publisher
    ..This led us to the conclusion that AC5, by binding active G?i1, interferes with G-protein deactivation and reassembly and thereby might sensitize its own regulation. ..
  12. Yang F, Wang M, Zhang B, Xiang W, Zhang K, Chu M, et al. Identification of new progestogen-associated networks in mammalian ovulation using bioinformatics. BMC Syst Biol. 2018;12:36 pubmed publisher
    ..q-PCR analysis following hCG and RU486 treatments confirmed the certain novel progestogenic-associated genes (GNAI1, PRKCA, CAV1, EGFR, RHOA, ZYX, VCL, GRB2 and RAP1A)...
  13. Malek R, Toman R, Edsall L, Wong S, Chiu J, Letterle C, et al. Nrg-1 belongs to the endothelial differentiation gene family of G protein-coupled sphingosine-1-phosphate receptors. J Biol Chem. 2001;276:5692-9 pubmed
    ..The antiproliferative effect, like S1P-mediated ERK1/2 inhibition, was orthovanadate-sensitive and pertussis toxin-insensitive. Our results indicate that EDG-8, a member of the EDG-1 subfamily, couples to unique signaling pathways. ..
  14. Wyckoff M, Chambliss K, Mineo C, Yuhanna I, Mendelsohn M, Mumby S, et al. Plasma membrane estrogen receptors are coupled to endothelial nitric-oxide synthase through Galpha(i). J Biol Chem. 2001;276:27071-6 pubmed
    ..Thus, novel G protein coupling enables a subpopulation of ERalpha to initiate signal transduction at the cell surface. Similar mechanisms may underly the nongenomic actions of other steroid hormones. ..
  15. Xiang B, Zhang G, Liu J, Morris A, Smyth S, Gartner T, et al. A G(i) -independent mechanism mediating Akt phosphorylation in platelets. J Thromb Haemost. 2010;8:2032-41 pubmed publisher
    ..Our results reveal a novel P2Y??-independent signaling pathway mediating Akt phosphorylation in response to thrombin receptors. ..
  16. Lamberts J, Jutkiewicz E, Mortensen R, Traynor J. ?-Opioid receptor coupling to G?(o) plays an important role in opioid antinociception. Neuropsychopharmacology. 2011;36:2041-53 pubmed publisher
    ..These results provide the first in vivo evidence that G?(o) contributes to maximally efficient MOR signaling and antinociception. ..
  17. Jia M, Li J, Zhu J, Wen W, Zhang M, Wang W. Crystal structures of the scaffolding protein LGN reveal the general mechanism by which GoLoco binding motifs inhibit the release of GDP from Gαi. J Biol Chem. 2012;287:36766-76 pubmed publisher
    ..Together with the sequence alignment, we suggest that the LGN GL/Gα(i) interaction represents a general binding mode between GL motifs and Gα(i). We also show that LGN GLs are potent guanine nucleotide dissociation inhibitors. ..
  18. Lamberts J, Smith C, Li M, Ingram S, Neubig R, Traynor J. Differential control of opioid antinociception to thermal stimuli in a knock-in mouse expressing regulator of G-protein signaling-insensitive G?o protein. J Neurosci. 2013;33:4369-77 pubmed publisher
  19. Lee C, Ahn H, Lee S, Shin J, Park W, Kim J, et al. Integrated analysis of omics data using microRNA-target mRNA network and PPI network reveals regulation of Gnai1 function in the spinal cord of Ews/Ewsr1 KO mice. BMC Med Genomics. 2016;9 Suppl 1:33 pubmed publisher
    ..On the biological side, we report that EWS regulates G-protein, Gnai1, in the spinal cord of Ews/Ewsr1 KO mice using the two biological network integrated analysis method...
  20. Nguyen L, Masouminia M, Mendoza A, Samadzadeh S, Tillman B, Morgan T, et al. Alcoholic hepatitis versus non-alcoholic steatohepatitis: Levels of expression of some proteins involved in tumorigenesis. Exp Mol Pathol. 2018;104:45-49 pubmed publisher
    ..of the Ingenuity Canonical Pathway of Molecular Mechanisms of Cancer and include: RAP2B, NAIP, FYN, PAK6, SUV39H1, GNAI1, BAX, E2F3, CKDN2B, BAK1, BCL2, DIABLO, RASGRF2, GNA15, PIK3CB, BRCA1, MAP2K1, BIRC3, CDK2, and ATM...
  21. Marty C, Kozasa T, Quinn M, Ye R. Activation state-dependent interaction between Galphai and p67phox. Mol Cell Biol. 2006;26:5190-200 pubmed
    ..These results provide the first evidence for an interaction between p67phox and an alpha subunit of heterotrimeric G proteins, suggesting a potential role for Galphai in the regulation or activation of NADPH oxidase. ..
  22. Cheung R, Ravyn V, Wang L, Ptasznik A, Collman R. Signaling mechanism of HIV-1 gp120 and virion-induced IL-1beta release in primary human macrophages. J Immunol. 2008;180:6675-84 pubmed
    ..This study identifies a novel signaling mechanism for HIV-1-induced IL-1beta production by primary human macrophages that may be involved in the neuropathogenesis of HIV-associated dementia. ..
  23. Lee M, Evans M, Hla T. The inducible G protein-coupled receptor edg-1 signals via the G(i)/mitogen-activated protein kinase pathway. J Biol Chem. 1996;271:11272-9 pubmed
    ..These data suggest that the G(i)/mitogen-activated protein kinase pathway is a major signaling pathway regulated by the orphan receptor edg-1. ..
  24. Brown E, Frazier W. Integrin-associated protein (CD47) and its ligands. Trends Cell Biol. 2001;11:130-5 pubmed
  25. Takesono A, Nowak M, Cismowski M, Duzic E, Lanier S. Activator of G-protein signaling 1 blocks GIRK channel activation by a G-protein-coupled receptor: apparent disruption of receptor signaling complexes. J Biol Chem. 2002;277:13827-30 pubmed
    ..Such regulation would be of particular importance for those receptors that exist precoupled to heterotrimeric G-protein and for receptors operating within signaling complexes. ..
  26. Mukhopadhyay S, Howlett A. CB1 receptor-G protein association. Subtype selectivity is determined by distinct intracellular domains. Eur J Biochem. 2001;268:499-505 pubmed
    ..These studies indicate that different domains of the CB(1) receptor direct the interaction with specific G protein subtypes. ..
  27. Liang M, Freedman N, Theiss C, Liggett S. Serine 232 of the alpha(2A)-adrenergic receptor is a protein kinase C-sensitive effector coupling switch. Biochemistry. 2001;40:15031-7 pubmed
    ..This represents one mechanism by which cells route stimuli directed to multifunctional receptors to selected effectors so as to attain finely targeted signaling. ..
  28. Daiter E, Makrigiannakis A, Coukos G, Woolkalis M, Coutifaris C. Inhibitory G protein alpha subunit (Gi alpha) expression and localization during human trophoblast differentiation. Eur J Obstet Gynecol Reprod Biol. 2000;88:165-9 pubmed
    ..These results suggest that Gi alpha is regulated and may be implicated in cAMP-dependent events during the terminal differentiation of human trophoblasts. ..
  29. Wang J, Sengupta P, Guo Y, Golebiewska U, Scarlata S. Evidence for a second, high affinity Gbetagamma binding site on Galphai1(GDP) subunits. J Biol Chem. 2009;284:16906-13 pubmed publisher
    ..We propose that this second binding site serves to promote and stabilize G protein subunit interactions in the presence of competing cellular proteins. ..
  30. Spear M, Guo J, Turner A, Yu D, Wang W, Meltzer B, et al. HIV-1 triggers WAVE2 phosphorylation in primary CD4 T cells and macrophages, mediating Arp2/3-dependent nuclear migration. J Biol Chem. 2014;289:6949-59 pubmed publisher
    ..Our results suggest that Arp2/3 and the upstream regulator, WAVE2, are essential co-factors hijacked by HIV for intracellular migration, and may serve as novel targets to prevent HIV transmission. ..
  31. Druey K, Sullivan B, Brown D, Fischer E, Watson N, Blumer K, et al. Expression of GTPase-deficient Gialpha2 results in translocation of cytoplasmic RGS4 to the plasma membrane. J Biol Chem. 1998;273:18405-10 pubmed
  32. Cismowski M, Ma C, Ribas C, Xie X, Spruyt M, Lizano J, et al. Activation of heterotrimeric G-protein signaling by a ras-related protein. Implications for signal integration. J Biol Chem. 2000;275:23421-4 pubmed
    ..AGS1 thus presents a unique mechanism for signal integration via heterotrimeric G-protein signaling pathways. ..
  33. Nebl T, Pestonjamasp K, Leszyk J, Crowley J, Oh S, Luna E. Proteomic analysis of a detergent-resistant membrane skeleton from neutrophil plasma membranes. J Biol Chem. 2002;277:43399-409 pubmed
    ..We suggest that the DRM-H fraction represents a membrane skeleton-associated subset of leukocyte signaling domains. ..
  34. Schiaffino M, d Addio M, Alloni A, Baschirotto C, Valetti C, Cortese K, et al. Ocular albinism: evidence for a defect in an intracellular signal transduction system. Nat Genet. 1999;23:108-12 pubmed
    ..Our data suggest that OA1 represents the first example of an exclusively intracellular GPCR and support the hypothesis that GPCR-mediated signal transduction systems also operate at the internal membranes in mammalian cells...
  35. Caricasole A, Sala C, Roncarati R, Formenti E, Terstappen G. Cloning and characterization of the human phosphoinositide-specific phospholipase C-beta 1 (PLC beta 1). Biochim Biophys Acta. 2000;1517:63-72 pubmed
    ..Finally, we characterized the structure of the PLC beta 1 locus and confirmed its mapping to human chromosome 20. ..
  36. Hurst J, Henkel P, Brown A, Hooks S. Endogenous RGS proteins attenuate Galpha(i)-mediated lysophosphatidic acid signaling pathways in ovarian cancer cells. Cell Signal. 2008;20:381-9 pubmed
    ..These data establish RGS proteins as novel regulators of LPA signaling in ovarian cancer cells. ..
  37. Moriya K, Kimoto M, Matsuzaki K, Kiwado A, Takamitsu E, Utsumi T. Identification of dually acylated proteins from complementary DNA resources by cell-free and cellular metabolic labeling. Anal Biochem. 2016;511:1-9 pubmed publisher
    ..As a result, EEPD1, one of five proteins (RFTN1, EEPD1, GNAI1, PDE2A, RNF11) found to be dually acylated, was shown to be a novel dually fatty acylated protein...
  38. Inanobe A, Fujita S, Makino Y, Matsushita K, Ishii M, Chachin M, et al. Interaction between the RGS domain of RGS4 with G protein alpha subunits mediates the voltage-dependent relaxation of the G protein-gated potassium channel. J Physiol. 2001;535:133-43 pubmed
    ..6. This study therefore shows that interaction between the RGS domain and PTX-sensitive G(alpha) subunits mediates the effect of RGS4 on the agonist concentration-dependent relaxation of K(G) channels. ..
  39. Dessauer C, Tesmer J, Sprang S, Gilman A. Identification of a Gialpha binding site on type V adenylyl cyclase. J Biol Chem. 1998;273:25831-9 pubmed
    ..The pseudosymmetrical structure of adenylyl cyclase permits bidirectional regulation of activity by homologous G protein alpha subunits. ..
  40. Wang Y, Young K. Analysis of RGSZ1 protein interaction with Galphai subunits. Methods Enzymol. 2004;390:31-52 pubmed
  41. Li H, Liang S. Local network topology in human protein interaction data predicts functional association. PLoS ONE. 2009;4:e6410 pubmed publisher
    ..Our study gives clear insight into the common neighbor-based prediction scheme and provides a reliable method for large-scale functional annotation in this post-genomic era. ..
  42. Grammatopoulos D, Dai Y, Randeva H, Levine M, Karteris E, Easton A, et al. A novel spliced variant of the type 1 corticotropin-releasing hormone receptor with a deletion in the seventh transmembrane domain present in the human pregnant term myometrium and fetal membranes. Mol Endocrinol. 1999;13:2189-202 pubmed
    ..Furthermore, CRH failed to stimulate inositol trisphosphate production. Coexpression studies between the CRH-R1d or CRH-R1alpha showed that this receptor does not play a role as a dominant negative receptor for CRH. ..
  43. Yeo A, Samways D, Fowler C, Gunn Moore F, Henderson G. Coincident signalling between the Gi/Go-coupled delta-opioid receptor and the Gq-coupled m3 muscarinic receptor at the level of intracellular free calcium in SH-SY5Y cells. J Neurochem. 2001;76:1688-700 pubmed
  44. Le Poul E, Loison C, Struyf S, Springael J, Lannoy V, Decobecq M, et al. Functional characterization of human receptors for short chain fatty acids and their role in polymorphonuclear cell activation. J Biol Chem. 2003;278:25481-9 pubmed
    ..GPR43 might therefore constitute a target allowing us to modulate immune responses in these pathological situations. ..
  45. Adhikari A, Sprang S. Thermodynamic characterization of the binding of activator of G protein signaling 3 (AGS3) and peptides derived from AGS3 with G alpha i1. J Biol Chem. 2003;278:51825-32 pubmed
  46. Willard F, McCudden C, Siderovski D. G-protein alpha subunit interaction and guanine nucleotide dissociation inhibitor activity of the dual GoLoco motif protein PCP-2 (Purkinje cell protein-2). Cell Signal. 2006;18:1226-34 pubmed
    ..Collectively, the data demonstrate that PCP-2 is a comparatively weak GoLoco motif protein that exhibits highest affinity interactions and GDI activity toward Galphai1, Galphai2, and Galphai3 subunits. ..
  47. Kotak S, Busso C, G nczy P. Cortical dynein is critical for proper spindle positioning in human cells. J Cell Biol. 2012;199:97-110 pubmed publisher
    ..Overall, we propose a model in which the ternary complex serves to anchor dynein at the plasma membrane to ensure correct spindle positioning...
  48. Kilts J, Akazawa T, El Moalem H, Mathew J, Newman M, Kwatra M. Age increases expression and receptor-mediated activation of G alpha i in human atria. J Cardiovasc Pharmacol. 2003;42:662-70 pubmed
    ..Because beta2ARs in human atria mediate contractility through cAMP-mediated phosphorylation of phospholamban, we conclude that an age-induced increase in Galphai may have a role in depressing cardiac function in aged human atria. ..
  49. Lau K, Ma F, Xia J, Chan Q, Ng C, To K. Activation of GPR30 stimulates GTP-binding of G?i1 protein to sustain activation of Erk1/2 in inhibition of prostate cancer cell growth and modulates metastatic properties. Exp Cell Res. 2017;350:199-209 pubmed publisher
    ..Taken together, our findings revealed the underlying mechanism for sustaining Erk1/2 activation upon GPR30 activation by G-1 in PCa cells and the GPR30-mediated pathways in controlling PCa cell growth and metastatic properties. ..
  50. Soundararajan M, Willard F, Kimple A, Turnbull A, Ball L, Schoch G, et al. Structural diversity in the RGS domain and its interaction with heterotrimeric G protein alpha-subunits. Proc Natl Acad Sci U S A. 2008;105:6457-62 pubmed publisher
  51. Sammond D, Eletr Z, Purbeck C, Kuhlman B. Computational design of second-site suppressor mutations at protein-protein interfaces. Proteins. 2010;78:1055-65 pubmed publisher
    ..In two cases, specificity was further tuned by including mutations distant from the initial design. Proteins 2010. (c) 2009 Wiley-Liss, Inc. ..
  52. Kapoor N, Gupta R, Menon S, Folta Stogniew E, Raleigh D, Sakmar T. Nucleobindin 1 is a calcium-regulated guanine nucleotide dissociation inhibitor of G{alpha}i1. J Biol Chem. 2010;285:31647-60 pubmed publisher
    ..We propose that cytoplasmic NUCB1 might function to regulate heterotrimeric G protein trafficking and G protein-coupled receptor-mediated signal transduction pathways. ..
  53. Kumar R, Tripathi V, Ahmad M, Nath N, Mir R, Chauhan S, et al. CXCR7 mediated Gi? independent activation of ERK and Akt promotes cell survival and chemotaxis in T cells. Cell Immunol. 2012;272:230-41 pubmed publisher
    ..Our findings suggest that CXCR7 promotes cell survival and does not induce cell death in T cells. The CXCL12 signaling via CXCR7 may be crucial in determining the fate of the activated T cells. ..
  54. Yang X, Mei S, Wang X, Li X, Liu R, Ma Y, et al. Leucine facilitates insulin signaling through a G?i protein-dependent signaling pathway in hepatocytes. J Biol Chem. 2013;288:9313-20 pubmed publisher
    ..This is the first evidence showing that macronutrients like amino acid leucine can facilitate insulin signaling through G proteins directly. ..
  55. Wieland T, Bahtijari N, Zhou X, Kleuss C, Simon M. Polarity exchange at the interface of regulators of G protein signaling with G protein alpha-subunits. J Biol Chem. 2000;275:28500-6 pubmed
    ..Nevertheless, they are sensitive to glutamate to lysine mutants of RGS proteins. Such mutant pairs will be helpful tools in analyzing Galpha-RGS specificities in living cells. ..
  56. Senogles S, Heimert T, Odife E, Quasney M. A region of the third intracellular loop of the short form of the D2 dopamine receptor dictates Gi coupling specificity. J Biol Chem. 2004;279:1601-6 pubmed
    ..These results suggest that this region of the third cytoplasmic loop is crucial for determining G(i) protein coupling specificity. ..
  57. Rey O, Young S, Yuan J, Slice L, Rozengurt E. Amino acid-stimulated Ca2+ oscillations produced by the Ca2+-sensing receptor are mediated by a phospholipase C/inositol 1,4,5-trisphosphate-independent pathway that requires G12, Rho, filamin-A, and the actin cytoskeleton. J Biol Chem. 2005;280:22875-82 pubmed
    ..Our model envisages that Ca(2+) or amino acids stabilize unique CaR conformations that favor coupling to different G proteins and subsequent activation of distinct downstream signaling pathways. ..
  58. Guillaume J, Daulat A, Maurice P, Levoye A, Migaud M, Brydon L, et al. The PDZ protein mupp1 promotes Gi coupling and signaling of the Mt1 melatonin receptor. J Biol Chem. 2008;283:16762-71 pubmed publisher
    ..Our findings highlight a previously unappreciated role of PDZ proteins in promoting G protein coupling to receptors. ..
  59. Sullivan K, Liao Y, Alborzi A, Beiderman B, Chang F, Masters S, et al. Inhibitory and stimulatory G proteins of adenylate cyclase: cDNA and amino acid sequences of the alpha chains. Proc Natl Acad Sci U S A. 1986;83:6687-91 pubmed
    ..Further comparison of the alpha-chain sequences suggests additional regions that may contribute to interactions with beta gamma subunits and the receptor and effector components of different signal transduction systems. ..
  60. Windh R, Lee M, Hla T, An S, Barr A, Manning D. Differential coupling of the sphingosine 1-phosphate receptors Edg-1, Edg-3, and H218/Edg-5 to the G(i), G(q), and G(12) families of heterotrimeric G proteins. J Biol Chem. 1999;274:27351-8 pubmed
    ..These studies represent the first characterization of S1P receptor activity through G proteins directly and establish fundamental differences in coupling. ..
  61. Zhou J, Moroi K, Nishiyama M, Usui H, Seki N, Ishida J, et al. Characterization of RGS5 in regulation of G protein-coupled receptor signaling. Life Sci. 2001;68:1457-69 pubmed
  62. Itoh Y, Kawamata Y, Harada M, Kobayashi M, Fujii R, Fukusumi S, et al. Free fatty acids regulate insulin secretion from pancreatic beta cells through GPR40. Nature. 2003;422:173-6 pubmed
    ..Our results indicate that GPR40 agonists and/or antagonists show potential for the development of new anti-diabetic drugs. ..
  63. Bray P, Carter A, Guo V, Puckett C, Kamholz J, Spiegel A, et al. Human cDNA clones for an alpha subunit of Gi signal-transduction protein. Proc Natl Acad Sci U S A. 1987;84:5115-9 pubmed
    ..These results suggest there are at least two classes of alpha i mRNA. ..
  64. Mahon M, Bonacci T, Divieti P, Smrcka A. A docking site for G protein ?? subunits on the parathyroid hormone 1 receptor supports signaling through multiple pathways. Mol Endocrinol. 2006;20:136-46 pubmed
    ..Herein, we define a domain on the PTH1R that is capable of binding G protein heterotrimeric complexes via direct Gbetagamma interactions. ..
  65. Gupta V, Bhandari D, Leyme A, Aznar N, Midde K, Lo I, et al. GIV/Girdin activates Gαi and inhibits Gαs via the same motif. Proc Natl Acad Sci U S A. 2016;113:E5721-30 pubmed publisher
    ..Our findings also illuminate how such modulation of two opposing Gα proteins integrates downstream signals and cellular responses. ..
  66. Bloch D, Bloch K, Iannuzzi M, Collins F, Neer E, Seidman J, et al. The gene for the alpha i1 subunit of human guanine nucleotide binding protein maps near the cystic fibrosis locus. Am J Hum Genet. 1988;42:884-8 pubmed
    ..11. The location of the alpha i1 gene proved to be distinct from that of the cystic fibrosis locus. ..
  67. Hanzal Bayer M, Renault L, Roversi P, Wittinghofer A, Hillig R. The complex of Arl2-GTP and PDE delta: from structure to function. EMBO J. 2002;21:2095-106 pubmed
    ..We suggest PDE delta to be a specific soluble transport factor for certain prenylated proteins and Arl2-GTP a regulator of PDE delta-mediated transport. ..
  68. Mueller A, Strange P. CCL3, acting via the chemokine receptor CCR5, leads to independent activation of Janus kinase 2 (JAK2) and Gi proteins. FEBS Lett. 2004;570:126-32 pubmed
    ..Gi protein activation was not prevented by inhibition of JAK, showing that heterotrimeric G protein activation and activation of the JAK/signal transducer and activator of transcription (STAT) pathway are independent of each other. ..
  69. Song K, Peterson Y, Freidman A, Blumer J, Sato M, Lanier S. Identification and characterization of a G-protein regulatory motif in WAVE1. FEBS Lett. 2006;580:1993-8 pubmed
    ..The docking of Gialpha within a WAVE1 scaffolding complex may facilitate dynamic cycling and/or targeting for efficient and localized control of actin polymerization. ..
  70. Horlock C, Shakib F, Jones N, Sewell H, Ghaemmaghami A. Differential protein expression by dendritic cells from atopic and non-atopic individuals after stimulation by the major house dust mite allergen Der p 1. Int Arch Allergy Immunol. 2009;150:237-51 pubmed publisher
    ..Further studies should now focus on establishing the biological relevance of these proteins as biomarkers in house dust mite allergy and their role in allergen induced Th2 cell differentiation. ..
  71. Vellano C, Maher E, Hepler J, Blumer J. G protein-coupled receptors and resistance to inhibitors of cholinesterase-8A (Ric-8A) both regulate the regulator of g protein signaling 14 RGS14·G?i1 complex in live cells. J Biol Chem. 2011;286:38659-69 pubmed publisher
    ..Together, these findings indicate that RGS14 can form complexes with GPCRs in cells that are dependent on G?(i/o) and that these RGS14·G?(i1)·GPCR complexes may be substrates for other signaling partners such as Ric-8A. ..
  72. Kim S, Jin J, Kunapuli S. Akt activation in platelets depends on Gi signaling pathways. J Biol Chem. 2004;279:4186-95 pubmed
  73. Sunahara R, Taussig R. Isoforms of mammalian adenylyl cyclase: multiplicities of signaling. Mol Interv. 2002;2:168-84 pubmed
  74. Sun D, Flock T, Deupi X, Maeda S, Matkovic M, Mendieta S, et al. Probing Gαi1 protein activation at single-amino acid resolution. Nat Struct Mol Biol. 2015;22:686-694 pubmed publisher
    ..Destabilization of helix α1, caused by rearrangement of this activation cluster, leads to the weakening of the interdomain interface and release of GDP. ..
  75. Laugwitz K, Allgeier A, Offermanns S, Spicher K, Van Sande J, Dumont J, et al. The human thyrotropin receptor: a heptahelical receptor capable of stimulating members of all four G protein families. Proc Natl Acad Sci U S A. 1996;93:116-20 pubmed
  76. Wang Y, Ho G, Zhang J, Nieuwenhuijsen B, Edris W, Chanda P, et al. Regulator of G protein signaling Z1 (RGSZ1) interacts with Galpha i subunits and regulates Galpha i-mediated cell signaling. J Biol Chem. 2002;277:48325-32 pubmed
    ..In summary, these data suggest that RGSZ1 serves as a GTPase accelerating protein for Galpha(i) and regulates Galpha(i)-mediated signaling, thus expanding the potential role of RGSZ1 in G protein-mediated cellular activities. ..
  77. Kimple R, Willard F, Hains M, Jones M, Nweke G, Siderovski D. Guanine nucleotide dissociation inhibitor activity of the triple GoLoco motif protein G18: alanine-to-aspartate mutation restores function to an inactive second GoLoco motif. Biochem J. 2004;378:801-8 pubmed
  78. Karteris E, Zervou S, Pang Y, Dong J, Hillhouse E, Randeva H, et al. Progesterone signaling in human myometrium through two novel membrane G protein-coupled receptors: potential role in functional progesterone withdrawal at term. Mol Endocrinol. 2006;20:1519-34 pubmed
    ..Our data indicate the presence of a novel signaling pathway mediated by mPRs that may result in a functional progestin withdrawal, shifting the balance from a quiescent state to one of contraction. ..
  79. Oldham W, Van Eps N, Preininger A, Hubbell W, Hamm H. Mechanism of the receptor-catalyzed activation of heterotrimeric G proteins. Nat Struct Mol Biol. 2006;13:772-7 pubmed
    ..These data provide the first direct observation of R*-mediated conformational changes in G proteins and define the structural basis for GDP release from G alpha. ..
  80. Willard F, Zheng Z, Guo J, Digby G, Kimple A, Conley J, et al. A point mutation to Galphai selectively blocks GoLoco motif binding: direct evidence for Galpha.GoLoco complexes in mitotic spindle dynamics. J Biol Chem. 2008;283:36698-710 pubmed publisher
    ..GoLoco motif protein complexes in microtubule dynamics and spindle function during cell division as well as to delineate potential roles for GoLoco motifs in receptor-mediated signal transduction. ..
  81. Kim J, Kim Y, Ko J. CK beta 8/CCL23 induces cell migration via the Gi/Go protein/PLC/PKC delta/NF-kappa B and is involved in inflammatory responses. Life Sci. 2010;86:300-8 pubmed publisher
  82. Chiu C, Monat C, Robitaille M, Lacomme M, Daulat A, Macleod G, et al. SAPCD2 Controls Spindle Orientation and Asymmetric Divisions by Negatively Regulating the Gαi-LGN-NuMA Ternary Complex. Dev Cell. 2016;36:50-62 pubmed publisher
    ..These results uncover SAPCD2 as a key regulator of the ternary complex controlling spindle orientation during morphogenesis and asymmetric cell divisions. ..
  83. Thomas P, Pang Y, Dong J. Membrane androgen receptor characteristics of human ZIP9 (SLC39A) zinc transporter in prostate cancer cells: Androgen-specific activation and involvement of an inhibitory G protein in zinc and MAP kinase signaling. Mol Cell Endocrinol. 2017;447:23-34 pubmed publisher
    ..The results show ZIP9 is a specific Gi coupled-mAR mediating testosterone-induced MAP kinase and zinc signaling in PC3-ZIP9 cells. ..
  84. Gennity J, Siess W. Thrombin inhibits the pertussis-toxin-dependent ADP-ribosylation of a novel soluble Gi-protein in human platelets. Biochem J. 1991;279 ( Pt 3):643-50 pubmed
  85. Gagnon A, Murray D, Leadley R. Cloning and characterization of a novel regulator of G protein signalling in human platelets. Cell Signal. 2002;14:595-606 pubmed
    ..RGS18 is expressed in platelet, leukocyte, and megakaryocyte cell lines and binds to endogenous Galphai1, Galphai2, Galphai3, and Galphaq but not Galphaz, Galphas or Galpha12 in vitro. ..
  86. Jordan J, He J, Eungdamrong N, Gomes I, Ali W, Nguyen T, et al. Cannabinoid receptor-induced neurite outgrowth is mediated by Rap1 activation through G(alpha)o/i-triggered proteasomal degradation of Rap1GAPII. J Biol Chem. 2005;280:11413-21 pubmed
    ..Thus, the G(alpha)o/i-coupled cannabinoid receptor, by regulating the proteasomal degradation of Rap1GAPII, activates Rap1 to induce neurite outgrowth. ..
  87. Badr G, Borhis G, Treton D, Moog C, Garraud O, Richard Y. HIV type 1 glycoprotein 120 inhibits human B cell chemotaxis to CXC chemokine ligand (CXCL) 12, CC chemokine ligand (CCL)20, and CCL21. J Immunol. 2005;175:302-10 pubmed
    ..The gp120-induced decrease in B cell chemotaxis and CD62 ligand expression, and increase in CD95-mediated B cell apoptosis probably have major deleterious effects on B cell responsiveness during HIV infection and in vaccination trials. ..
  88. Lee J, Duk Jung I, Gyo Park C, Han J, Young Lee H. Autotaxin stimulates urokinase-type plasminogen activator expression through phosphoinositide 3-kinase-Akt-nuclear [corrected] factor kappa B signaling cascade in human melanoma cells. Melanoma Res. 2006;16:445-52 pubmed
    ..Collectively, these data strongly suggest autotaxin induces uPA expression via the Gi-PI3K-Akt-NF-kappaB signaling pathway that might be critical for autotaxin-induced tumor cell invasion and metastasis. ..
  89. Beqollari D, Betzenhauser M, Kammermeier P. Altered G-protein coupling in an mGluR6 point mutant associated with congenital stationary night blindness. Mol Pharmacol. 2009;76:992-7 pubmed publisher
    ..Most importantly, our results indicate a switch in G-protein coupling, in which E775K loses G(o) coupling but retains coupling to G(i), which may explain the phenotype. ..
  90. Watts A, Verkaar F, van der Lee M, Timmerman C, Kuijer M, van Offenbeek J, et al. ?-Arrestin recruitment and G protein signaling by the atypical human chemokine decoy receptor CCX-CKR. J Biol Chem. 2013;288:7169-81 pubmed publisher
    ..On the other hand, recruitment of the putative signaling scaffold ?-arrestin to CCX-CKR in response to chemokines might allow activation of yet to be identified signal transduction pathways. ..
  91. Zhu J, Chin K, Aerbajinai W, Kumkhaek C, Li H, Rodgers G. Hydroxyurea-inducible SAR1 gene acts through the Gi?/JNK/Jun pathway to regulate ?-globin expression. Blood. 2014;124:1146-56 pubmed publisher
    ..These results indicate that HU induces SAR1, which in turn activates ?-globin expression, predominantly through the Gi?/JNK/Jun pathway. Our findings identify SAR1 as an alternative therapeutic target for ?-globin disorders. ..
  92. Takahashi N, Wakasugi K. Identification of residues crucial for the interaction between human neuroglobin and the ?-subunit of heterotrimeric Gi protein. Sci Rep. 2016;6:24948 pubmed publisher
    ..Moreover, we show that Lys46, Lys70, Arg208, Lys209, and Lys210 residues of G?i1 are important for binding to human Ngb. We propose a molecular docking model of the complex between human Ngb and G?i1. ..