glutaredoxin 5

Summary

Gene Symbol: glutaredoxin 5
Description: glutaredoxin 5
Alias: C14orf87, FLB4739, GRX5, PR01238, PRO1238, glutaredoxin 5 homolog, glutaredoxin-related protein 5, mitochondrial, monothiol glutaredoxin-5
Species: human

Top Publications

  1. pmc Glutaredoxin 5 deficiency causes sideroblastic anemia by specifically impairing heme biosynthesis and depleting cytosolic iron in human erythroblasts
    Hong Ye
    Molecular Medicine Program, National Institute of Child Health and Human Development, NIH, Bethesda, Maryland 20892, USA
    J Clin Invest 120:1749-61. 2010
  2. ncbi Thioltransferase (glutaredoxin) is detected within HIV-1 and can regulate the activity of glutathionylated HIV-1 protease in vitro
    D A Davis
    HIV and AIDS Malignancy Branch, NCI, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 272:25935-40. 1997
  3. pmc Monothiol glutaredoxins can bind linear [Fe3S4]+ and [Fe4S4]2+ clusters in addition to [Fe2S2]2+ clusters: spectroscopic characterization and functional implications
    Bo Zhang
    Department of Chemistry and Center for Metalloenzyme Studies, University of Georgia, Athens, Georgia 30602, United States
    J Am Chem Soc 135:15153-64. 2013
  4. pmc The effects of mitochondrial iron homeostasis on cofactor specificity of superoxide dismutase 2
    Mei Yang
    Division of Toxicological Sciences, Department of Environmental Health Sciences, The Johns Hopkins University Bloomberg School of Public Health, Baltimore, MD 21205, USA
    EMBO J 25:1775-83. 2006
  5. doi Interaction between Saccharomyces cerevisiae glutaredoxin 5 and SPT10 and their in vivo functions
    Young Mee Oh
    Department of Biochemistry, Paichai University, Taejon 302 735, Republic of Korea
    Free Radic Biol Med 52:1519-30. 2012
  6. doi The role of mitochondria in cellular iron-sulfur protein biogenesis and iron metabolism
    Roland Lill
    Institut für Zytobiologie und Zytopathologie, Philipps Universitat Marburg, Robert Koch Str 6, 35033 Marburg, Germany
    Biochim Biophys Acta 1823:1491-508. 2012
  7. pmc BOLA1 is an aerobic protein that prevents mitochondrial morphology changes induced by glutathione depletion
    Peter Willems
    Department of Biochemistry, Nijmegen Centre for Molecular Life Sciences, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands
    Antioxid Redox Signal 18:129-38. 2013
  8. ncbi Plasmodium falciparum glutaredoxin-like proteins
    Marcel Deponte
    Interdisciplinary Research Center, Justus Liebig University, Heinrich Buff Ring 26 32, D 35392 Giessen, Germany
    Biol Chem 386:33-40. 2005
  9. pmc The mitochondrial Hsp70 chaperone Ssq1 facilitates Fe/S cluster transfer from Isu1 to Grx5 by complex formation
    Marta A Uzarska
    Institut für Zytobiologie und Zytopathologie, Philipps Universitat Marburg, 35032 Marburg, Germany
    Mol Biol Cell 24:1830-41. 2013
  10. pmc Gamma-tocotrienol modulated gene expression in senescent human diploid fibroblasts as revealed by microarray analysis
    Suzana Makpol
    Department of Biochemistry, Faculty of Medicine, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, 50300 Kuala Lumpur, Malaysia
    Oxid Med Cell Longev 2013:454328. 2013

Scientific Experts

  • Ning Hui Cheng
  • Rui Alves
  • E Herrero
  • Clara Camaschella
  • Suzana Makpol
  • Roland Lill
  • Anna Lewinska
  • Catrine Johansson
  • GABRIEL ROBERT LINARES
  • Gemma Belli
  • GABRIEL LINARES
  • Kyoung Dong Kim
  • Hong Ye
  • Bo Zhang
  • Priyanka Shakamuri
  • Jordi Tamarit
  • Michael K Johnson
  • Sibali Bandyopadhyay
  • Jung Hye Roe
  • Amornrat Naranuntarat
  • Maria Teresa Rodriguez-Manzaneque
  • Woo Hyun Chung
  • Maria Micaela Molina-Navarro
  • Peter Willems
  • Nuria Pujol-Carrion
  • Tohru Fujiwara
  • Marta A Uzarska
  • Xi Liu
  • Rocio V Pérez-Gallardo
  • Young Mee Oh
  • Jana Kucerova
  • María Micaela Molina
  • Yujeong Kim
  • Nicolas Rouhier
  • Boi Hanh Huynh
  • Sunil G Naik
  • Kyung Chang Lee
  • Hyo Jin Kim
  • Yeissa Chabrier-Roselló
  • Elena Garre
  • Ayse Banu Demir
  • Anke K Bergmann
  • Tracey A Rouault
  • Yi Wang
  • Gabriel R Linares
  • Ahmet Koc
  • Joaquim Ros
  • Michael Filser
  • E V Kalinina
  • Valeria C Culotta
  • Ercan Selcuk Unlu
  • Johan Sagemark
  • Ana Garcera
  • Arne Holmgren
  • Lidia Piedrafita
  • Mei Yang
  • Eun Hee Park
  • Marcel Deponte
  • Jeong Su Moon
  • Aristi Potamitou Fernandes
  • Chang Jin Lim
  • Hong Gyum Kim
  • Rebecca A Wingert
  • María Angeles De la Torre
  • Alma L Díaz-Pérez
  • Radek Szklarczyk
  • Sven Andreas Freibert
  • Martijn A Huynen
  • Leo Nijtmans
  • Isabel Duarte
  • Luis Serrano
  • Urszula Kudla
  • Jose S Rodriguez-Zavala
  • Yingang Feng
  • Kendal D Hirschi
  • Shian Liu
  • Werner Koopman
  • Jesús Campos-García
  • Sergio Gutiérrez
  • Jeremy Couturier
  • Haiteng Deng
  • Bas F J Wanschers
  • Hideo Harigae
  • Marleen Forkink
  • Luis S Briones
  • Jian Zhong Liu
  • Herman Swarts
  • Mima I Petkova
  • Jolein Gloerich
  • Maria Angeles de la Torre-Ruiz

Detail Information

Publications57

  1. pmc Glutaredoxin 5 deficiency causes sideroblastic anemia by specifically impairing heme biosynthesis and depleting cytosolic iron in human erythroblasts
    Hong Ye
    Molecular Medicine Program, National Institute of Child Health and Human Development, NIH, Bethesda, Maryland 20892, USA
    J Clin Invest 120:1749-61. 2010
    b>Glutaredoxin 5 (GLRX5) deficiency has previously been identified as a cause of anemia in a zebrafish model and of sideroblastic anemia in a human patient...
  2. ncbi Thioltransferase (glutaredoxin) is detected within HIV-1 and can regulate the activity of glutathionylated HIV-1 protease in vitro
    D A Davis
    HIV and AIDS Malignancy Branch, NCI, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 272:25935-40. 1997
    ..Our results implicate thioltransferase in the regulation and/or maintenance of protease activity in HIV-1 infected cells...
  3. pmc Monothiol glutaredoxins can bind linear [Fe3S4]+ and [Fe4S4]2+ clusters in addition to [Fe2S2]2+ clusters: spectroscopic characterization and functional implications
    Bo Zhang
    Department of Chemistry and Center for Metalloenzyme Studies, University of Georgia, Athens, Georgia 30602, United States
    J Am Chem Soc 135:15153-64. 2013
    Saccharomyces cerevisiae mitochondrial glutaredoxin 5 (Grx5) is the archetypical member of a ubiquitous class of monothiol glutaredoxins with a strictly conserved CGFS active-site sequence that has been shown to function in biological [..
  4. pmc The effects of mitochondrial iron homeostasis on cofactor specificity of superoxide dismutase 2
    Mei Yang
    Division of Toxicological Sciences, Department of Environmental Health Sciences, The Johns Hopkins University Bloomberg School of Public Health, Baltimore, MD 21205, USA
    EMBO J 25:1775-83. 2006
    ..cerevisiae mtm1, ssq1 and grx5, iron accumulates in a reactive form that potently competes with manganese for binding to SOD2, inactivating the ..
  5. doi Interaction between Saccharomyces cerevisiae glutaredoxin 5 and SPT10 and their in vivo functions
    Young Mee Oh
    Department of Biochemistry, Paichai University, Taejon 302 735, Republic of Korea
    Free Radic Biol Med 52:1519-30. 2012
    b>Glutaredoxin 5 (Grx5) is a monothiol member of the Grx family that comprises two dithiol and three monothiol members...
  6. doi The role of mitochondria in cellular iron-sulfur protein biogenesis and iron metabolism
    Roland Lill
    Institut für Zytobiologie und Zytopathologie, Philipps Universitat Marburg, Robert Koch Str 6, 35033 Marburg, Germany
    Biochim Biophys Acta 1823:1491-508. 2012
    ..Isu1 and transferred toward apoproteins with the help of a dedicated Hsp70 chaperone system and the glutaredoxin Grx5. Finally, various specialized ISC components assist in the generation of [4Fe-4S] clusters and cluster insertion ..
  7. pmc BOLA1 is an aerobic protein that prevents mitochondrial morphology changes induced by glutathione depletion
    Peter Willems
    Department of Biochemistry, Nijmegen Centre for Molecular Life Sciences, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands
    Antioxid Redox Signal 18:129-38. 2013
    ..In Escherichia coli, BolA causes a spherical cell shape and is overexpressed during oxidative stress. Here we aim to elucidate the possible role of its human homolog BOLA1 in mitochondrial morphology and thiol redox potential regulation...
  8. ncbi Plasmodium falciparum glutaredoxin-like proteins
    Marcel Deponte
    Interdisciplinary Research Center, Justus Liebig University, Heinrich Buff Ring 26 32, D 35392 Giessen, Germany
    Biol Chem 386:33-40. 2005
    ..Yeast Grx5 is the only glutaredoxin-like protein studied biochemically so far...
  9. pmc The mitochondrial Hsp70 chaperone Ssq1 facilitates Fe/S cluster transfer from Isu1 to Grx5 by complex formation
    Marta A Uzarska
    Institut für Zytobiologie und Zytopathologie, Philipps Universitat Marburg, 35032 Marburg, Germany
    Mol Biol Cell 24:1830-41. 2013
    ..Here we use in vivo and in vitro approaches to show that Ssq1 also interacts with the monothiol glutaredoxin 5 (Grx5) at a binding site different from that of Isu1...
  10. pmc Gamma-tocotrienol modulated gene expression in senescent human diploid fibroblasts as revealed by microarray analysis
    Suzana Makpol
    Department of Biochemistry, Faculty of Medicine, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, 50300 Kuala Lumpur, Malaysia
    Oxid Med Cell Longev 2013:454328. 2013
    ..These findings revealed that γ -tocotrienol may prevent cellular aging of human diploid fibroblasts by modulating gene expression...
  11. pmc Monothiol glutaredoxins function in storing and transporting [Fe2S2] clusters assembled on IscU scaffold proteins
    Priyanka Shakamuri
    Department of Chemistry and Center for Metalloenzyme Studies, University of Georgia, Athens, Georgia 30602, USA
    J Am Chem Soc 134:15213-6. 2012
    ..In this work, cluster transfer from Azotobacter vinelandii [Fe(2)S(2)](2+) cluster-bound IscU to apo-Grx5, a general purpose monothiol glutaredoxin in A...
  12. doi Reactive oxygen species production induced by ethanol in Saccharomyces cerevisiae increases because of a dysfunctional mitochondrial iron-sulfur cluster assembly system
    Rocio V Pérez-Gallardo
    Lab de Biotecnología Microbiana, Instituto de Investigaciones Quimico Biologicas, Morelia, Michoacan, Mexico
    FEMS Yeast Res 13:804-19. 2013
    ..were increased in yeast treated with ethanol and increased to a higher level in the ssq1∆, isa1∆, iba57∆ and grx5∆ mutants - hydrogen peroxide and superoxide were the main molecules detected...
  13. ncbi Structure-function analysis of yeast Grx5 monothiol glutaredoxin defines essential amino acids for the function of the protein
    Gemma Belli
    Departament de Ciencies Mediques Basiques, Facultat de Medicina, Universitat de Lleida, Rovira Roure 44, 25198 Lleida, Spain
    J Biol Chem 277:37590-6. 2002
    b>Grx5 defines a family of yeast monothiol glutaredoxins that also includes Grx3 and Grx4. All three proteins display significant sequence homology with proteins found from bacteria to humans...
  14. doi Pathophysiology and genetic mutations in congenital sideroblastic anemia
    Tohru Fujiwara
    Department of Hematology and Rheumatology, Tohoku University Graduate School of Medicine, Sendai, Japan Molecular Hematology Oncology, Tohoku University Graduate School of Medicine, Sendai, Japan
    Pediatr Int 55:675-9. 2013
    ..specific mitochondrial transporter (SLC25A38), adenosine triphosphate (ATP) binding cassette B7 (ABCB7), glutaredoxin 5 (GLRX5), thiamine transporter SLC19A2, the RNA-modifying enzyme pseudouridine synthase (PUS1), and ..
  15. pmc The MAP kinase Slt2 is involved in vacuolar function and actin remodeling in Saccharomyces cerevisiae mutants affected by endogenous oxidative stress
    Nuria Pujol-Carrion
    Department de Ciencies Mediques Basiques, Institut de Recerca Biomèdica, Universidad de Lleida, Lleida, Spain
    Appl Environ Microbiol 79:6459-71. 2013
    ..In this study, we show that grx3 grx4 and grx5 mutants are cellular models of endogenous oxidative stress...
  16. pmc Structural insights into the N-terminal GIY-YIG endonuclease activity of Arabidopsis glutaredoxin AtGRXS16 in chloroplasts
    Xi Liu
    Ministry of Education Key Laboratory of Protein Science, Center for Structural Biology, School of Life Sciences, Tsinghua University, Beijing 100084, China
    Proc Natl Acad Sci U S A 110:9565-70. 2013
    ..analysis indicated that AtGRXS16-NTD could inhibit the ability of AtGRXS16 to suppress the sensitivity of yeast grx5 cells to oxidative stress; however, the C-terminal Grx domain itself and AtGRXS16 with a Cys123Ser mutation were ..
  17. pmc The interaction of mitochondrial iron with manganese superoxide dismutase
    Amornrat Naranuntarat
    Department of Environmental Health Sciences, Johns Hopkins University Bloomberg School of Public Health, Baltimore, Maryland 21205, USA
    J Biol Chem 284:22633-40. 2009
    ..when cells are starved for manganese or when mitochondrial iron homeostasis is disrupted by mutations in yeast grx5, ssq1, and mtm1...
  18. pmc Systematic molecular genetic analysis of congenital sideroblastic anemia: evidence for genetic heterogeneity and identification of novel mutations
    Anke K Bergmann
    Division of Hematology Oncology, Children s Hospital Boston, Harvard Medical School, Boston, Massachusetts, USA
    Pediatr Blood Cancer 54:273-8. 2010
    ..enzyme pseudouridine synthase 1 (PUS1), a mitochondrial ATP-binding cassette transporter (ABCB7), glutaredoxin 5 (GLRX5), as well as mitochondrial DNA deletions...
  19. doi Hereditary sideroblastic anemias: pathophysiology, diagnosis, and treatment
    Clara Camaschella
    Vita Salute University and San Raffaele Scientific Institute, Milan, Italy
    Semin Hematol 46:371-7. 2009
    ..The identification of other genes, such as adenosine triphosphate (ATP) binding cassette B7 (ABCB7) and glutaredoxin 5 (GLRX5), has strengthened the role of iron sulfur cluster biogenesis in sideroblast formation and revealed a ..
  20. doi Oxidative stress responses and lipid peroxidation damage are induced during dehydration in the production of dry active wine yeasts
    Elena Garre
    Departamento de Bioquimica y Biologia Molecular, Universitat de Valencia, Valencia, Spain
    Int J Food Microbiol 136:295-303. 2010
    ..The main gene expression response involves the induction of genes TRR1 and GRX5, corresponding to the two main redox balance systems, thioredoxins and glutathione/glutaredoxins...
  21. doi Monothiol glutaredoxin Grx5 interacts with Fe-S scaffold proteins Isa1 and Isa2 and supports Fe-S assembly and DNA integrity in mitochondria of fission yeast
    Kyoung Dong Kim
    Institute of Microbiology, Seoul National University, Seoul 151 742, South Korea
    Biochem Biophys Res Commun 392:467-72. 2010
    ..Among three monothiol glutaredoxins (Grx3, 4, and 5) in Schizosaccharomyces pombe only Grx5 resides in mitochondria...
  22. pmc Inhibition of electron transport chain assembly and function promotes photodynamic killing of Candida
    Yeissa Chabrier-Roselló
    Department of Microbiology and Immunology, University of Rochester Medical Center, Box 672, 601 Elmwood Ave, Rochester, NY 14642, USA
    J Photochem Photobiol B 99:117-25. 2010
    ..A S. cerevisiae mitochondrial glutaredoxin grx5 mutant, defective in assembly of Fe-S clusters critical for Complex III function, displayed increased sensitivity ..
  23. pmc Human iron-sulfur cluster assembly, cellular iron homeostasis, and disease
    Hong Ye
    Molecular Medicine Program, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892, USA
    Biochemistry 49:4945-56. 2010
    ..However, the human diseases, Friedreich ataxia, glutaredoxin 5-deficient sideroblastic anemia, ISCU myopathy, and ABCB7 sideroblastic anemia/ataxia syndrome, affect specific ..
  24. doi Assessment of chronological lifespan dependent molecular damages in yeast lacking mitochondrial antioxidant genes
    Ayse Banu Demir
    Department of Molecular Biology and Genetics, Izmir Institute of Technology, 35430 Urla, Izmir, Turkey
    Biochem Biophys Res Commun 400:106-10. 2010
    ..aged mutants lacking the mitochondrial antioxidant genes: GRX2, CCP1, SOD1, GLO4, TRR2, TRX3, CCS1, SOD2, GRX5, and PRX1...
  25. doi The crystal structure of human GLRX5: iron-sulfur cluster co-ordination, tetrameric assembly and monomer activity
    Catrine Johansson
    University of Oxford, UK
    Biochem J 433:303-11. 2011
    Human GLRX5 (glutaredoxin 5) is an evolutionarily conserved thiol-disulfide oxidoreductase that has a direct role in the maintenance of normal cytosolic and mitochondrial iron homoeostasis, and its expression affects haem biosynthesis and ..
  26. ncbi Structural and functional diversity of glutaredoxins in yeast
    Enrique Herrero
    Departament de Ciencies Mediques Basiques, IRBLLEIDA, Universitat de Lleida, Montserrat Roig 2, 25008 Lleida, Spain
    Curr Protein Pept Sci 11:659-68. 2010
    ..subfamily in Saccharomyces cerevisiae participate in the synthesis of the iron-sulfur clusters in mitochondria (Grx5), or in signalling the iron status inside the cell for regulation of iron uptake and intracellular iron ..
  27. doi New mutation in erythroid-specific delta-aminolevulinate synthase as the cause of X-linked sideroblastic anemia responsive to pyridoxine
    Jana Kucerova
    Department of Biology, Faculty of Medicine, Palacky University, Olomouc, Czech Republic
    Acta Haematol 125:193-7. 2011
    ..In addition, autosomal recessive mutations in the erythroid-specific mitochondrial transporter SLC25A38 and glutaredoxin 5 (GLRX5) have recently been identified in SA patients with isolated erythroid phenotype.
  28. doi Redox regulation of the tumor suppressor PTEN by glutaredoxin 5 and Ycp4
    Yujeong Kim
    Department of Biochemistry, Research Center for Aging and Geriatrics, Research Institute of Medical Sciences, Chonnam National University Medical School, Gwangju 501 190, Republic of Korea
    Biochem Biophys Res Commun 407:175-80. 2011
    ..Reduction of hPTEN was delayed in each of S. cerevisiae grx5Δ and ycp4Δ mutants...
  29. doi Multi-domain CGFS-type glutaredoxin Grx4 regulates iron homeostasis via direct interaction with a repressor Fep1 in fission yeast
    Kyoung Dong Kim
    Laboratory of Molecular Microbiology, School of Biological Sciences and Institute of Microbiology, Seoul National University, Seoul, Republic of Korea
    Biochem Biophys Res Commun 408:609-14. 2011
    The fission yeast Schizosaccharomyces pombe contains two CGFS-type monothiol glutaredoxins, Grx4 and Grx5, which are localized primarily in the nucleus and mitochondria, respectively...
  30. ncbi Nuclear monothiol glutaredoxins of Saccharomyces cerevisiae can function as mitochondrial glutaredoxins
    María Micaela Molina
    Departament de Ciencies Mediques Basiques, Facultat de Medicina, Universitat de Lleida, Montserrat Roig 2, 25008 Lleida, Spain
    J Biol Chem 279:51923-30. 2004
    ..In Saccharomyces cerevisiae, Grx1 and Grx2 are cytosolic dithiol glutaredoxins, whereas Grx3, Grx4, and Grx5 are monothiol glutaredoxins...
  31. pmc Cloning, overproduction, purification, crystallization and preliminary X-ray diffraction analysis of yeast glutaredoxin Grx5
    Yi Wang
    Hefei National Laboratory for Physical Sciences at Microscale and School of Life Sciences, University of Science and Technology of China, Hefei, Anhui 230026, People s Republic of China
    Acta Crystallogr Sect F Struct Biol Cryst Commun 65:651-3. 2009
    b>Grx5 from the yeast Saccharomyces cerevisiae is a monothiol glutaredoxin that is involved in iron-sulfur cluster biogenesis. Here, yeast Grx5 was cloned and overproduced in Escherichia coli...
  32. doi Glutaredoxin 5 regulates osteoblast apoptosis by protecting against oxidative stress
    Gabriel R Linares
    Musculoskeletal Disease Center, Jerry L Pettis Memorial Veterans Affairs Medical Center, Loma Linda, CA 92357, USA
    Bone 44:795-804. 2009
    ..Osteoblasts were transfected with Grx5 siRNA and treated with hydrogen peroxide (H(2)O(2))...
  33. pmc Saccharomyces cerevisiae cells have three Omega class glutathione S-transferases acting as 1-Cys thiol transferases
    Ana Garcera
    Departament de Ciencies Mediques Basiques, Facultat de Medicina, Universitat de Lleida, Montserrat Roig 2, 25008 Lleida, Spain
    Biochem J 398:187-96. 2006
    ..Replacing the active site of the yeast monothiol glutaredoxin Grx5 with the proposed Gto2 active site containing Cys46 allows Grx5 to retain some activity against HED...
  34. ncbi Prokaryotic and eukaryotic monothiol glutaredoxins are able to perform the functions of Grx5 in the biogenesis of Fe/S clusters in yeast mitochondria
    Maria Micaela Molina-Navarro
    Departament de Ciencies Mediques Basiques, Facultat de Medicina, Universitat de Lleida, Montserrat Roig 2, 25008 Lleida, Spain
    FEBS Lett 580:2273-80. 2006
    The Saccharomyces cerevisiae monothiol glutaredoxin Grx5 participates in the mitochondrial biogenesis of iron-sulfur clusters. Grx5 homologues exist in organisms from bacteria to humans...
  35. ncbi Characterization and regulation of the gene encoding monothiol glutaredoxin 3 in the fission yeast Schizosaccharomyces pombe
    Jeong Su Moon
    Division of Life Sciences, College of Natural Sciences, Kangwon National University, Chuncheon 200 701, Korea
    Mol Cells 20:74-82. 2005
    ..3 kDa. The putative protein has 27% homology with Grx5, and contains many hydrophobic amino acid residues in its N-terminal region. S...
  36. ncbi The fission yeast gene encoding monothiol glutaredoxin 5 is regulated by nitrosative and osmotic stresses
    Hong Gyum Kim
    Division of Life Sciences, College of Natural Sciences, Kangwon National University, Chuncheon 200 701, Korea
    Mol Cells 20:43-50. 2005
    ..We have cloned the monothiol Grx5 gene from the genomic DNA of the fission yeast Schizosaccharomyces pombe...
  37. ncbi Deficiency of glutaredoxin 5 reveals Fe-S clusters are required for vertebrate haem synthesis
    Rebecca A Wingert
    Stem Cell Program and Division Hematology Oncology Children s Hospital and Dana Farber Cancer Institute, Howard Hughes Medical Institute, Harvard Medical School, Boston, Massachusetts 02115, USA
    Nature 436:1035-39. 2005
    ..Here we show that the hypochromic anaemia in shiraz (sir) zebrafish mutants is caused by deficiency of glutaredoxin 5 (grx5), a gene required in yeast for Fe-S cluster assembly...
  38. ncbi A novel monothiol glutaredoxin (Grx4) from Escherichia coli can serve as a substrate for thioredoxin reductase
    Aristi Potamitou Fernandes
    Medical Nobel Institute for Biochemistry, Department of Medical Biochemistry and Biophysics, Karolinska Institutet, S 171 77 Stockholm, Sweden
    J Biol Chem 280:24544-52. 2005
    ..potential active site and shows high sequence homology to the other monothiol glutaredoxins and especially to yeast Grx5. Experiments with gene knock-out techniques showed that the reading frame encoding Grx4 was essential...
  39. ncbi Localization and function of three monothiol glutaredoxins in Schizosaccharomyces pombe
    Woo Hyun Chung
    Laboratory of Molecular Microbiology, School of Biological Sciences, Institute of Microbiology, Seoul National University, Seoul 151 742, Republic of Korea
    Biochem Biophys Res Commun 330:604-10. 2005
    ..revealed that Grx3 is targeted to nuclear rim and endoplasmic reticulum, Grx4 primarily to the nucleus, and Grx5 to mitochondria...
  40. ncbi Predictive reconstruction of the mitochondrial iron-sulfur cluster assembly metabolism. II. Role of glutaredoxin Grx5
    Rui Alves
    Departament de Ciencies Mediques Basiques, Universidad de Lleida, Lleida, Spain
    Proteins 57:481-92. 2004
    b>Grx5 is a Saccharomyces cerevisiae glutaredoxin involved in iron-sulfur cluster (FeSC) biogenesis...
  41. ncbi Saccharomyces cerevisiae glutaredoxin 5-deficient cells subjected to continuous oxidizing conditions are affected in the expression of specific sets of genes
    Gemma Belli
    Departament de Ciencies Mediques Basiques, Facultat de Medicina, Universitat de Lleida, Rovira Roure 44, 25198 Lleida, Spain
    J Biol Chem 279:12386-95. 2004
    The Saccharomyces cerevisiae GRX5 gene codes for a mitochondrial glutaredoxin involved in the synthesis of iron/sulfur clusters...
  42. ncbi Biochemical characterization of yeast mitochondrial Grx5 monothiol glutaredoxin
    Jordi Tamarit
    Departament de Ciencies Mediques Basiques, Facultat de Medicina, Universitat de Lleida, Rovira Roure 44, 25198 Lleida, Spain
    J Biol Chem 278:25745-51. 2003
    b>Grx5 is a yeast mitochondrial protein involved in iron-sulfur biogenesis that belongs to a recently described family of monothiolic glutaredoxin-like proteins. No member of this family has been biochemically characterized previously...
  43. pmc Grx5 is a mitochondrial glutaredoxin required for the activity of iron/sulfur enzymes
    Maria Teresa Rodriguez-Manzaneque
    Departament de Ciencies Mediques Basiques, Facultat de Medicina, Universitat de Lleida, 25198 Lleida, Spain
    Mol Biol Cell 13:1109-21. 2002
    Yeast cells contain a family of three monothiol glutaredoxins: Grx3, 4, and 5. Absence of Grx5 leads to constitutive oxidative damage, exacerbating that caused by external oxidants...
  44. ncbi AtGRXcp, an Arabidopsis chloroplastic glutaredoxin, is critical for protection against protein oxidative damage
    Ning Hui Cheng
    Plant Physiology Group, United States Department of Agriculture Agricultural Research Service Children s Nutrition Research Center, Department of Pediatrics, Baylor College of Medicine, Houston, Texas 77030, USA
    J Biol Chem 281:26280-8. 2006
    ..demonstrate that a plant protein, AtGRXcp, is a chloroplast-localized monothiol Grx with high similarity to yeast Grx5. In yeast expression assays, AtGRXcp localized to the mitochondria and suppressed the sensitivity of yeast grx5 ..
  45. ncbi Glutaredoxins in fungi
    Enrique Herrero
    Departament de Ciencies Mediques Basiques, Facultat de Medicina, Universitat de Lleida, Montserrat Roig 2, 25008, Lleida, Spain
    Photosynth Res 89:127-40. 2006
    ..The function of yeast Grx5 seems to be conserved along the evolutionary scale.
  46. ncbi Involvement of thio-, peroxi-, and glutaredoxins in cellular redox-dependent processes
    E V Kalinina
    Center for Theoretical Problems of Physico Chemical Pharmacology, Russian Academy of Sciences, Moscow, 119991, Russia
    Biochemistry (Mosc) 73:1493-510. 2008
    ..Glutaredoxin, whose major isoforms in mammals are Grx1, Grx2, and Grx5, as well as thioredoxin, catalyzes S-glutathionylation and deglutathionylation of proteins to protect SH-groups ..
  47. doi Recent advances in the understanding of inherited sideroblastic anaemia
    Clara Camaschella
    Vita Salute San Raffaele University and San Raffaele Scientific Institute, Milan, Italy
    Br J Haematol 143:27-38. 2008
    ..Local mitochondrial iron overload is present in all sideroblastic anaemias, whereas systemic iron overload occurs only in the forms because of primary or secondary deficiency of ALAS2...
  48. doi A role for yeast glutaredoxin genes in selenite-mediated oxidative stress
    Anna Lewinska
    Department of Biochemistry and Cell Biology, University of Rzeszow, Cegielniana 12, PL 35 959 Rzeszow, Poland
    Fungal Genet Biol 45:1182-7. 2008
    ..this phenomenon and establish the roles of other components of yeast glutaredoxin system, in particular glutaredoxin 5 in the selenite resistance...
  49. pmc Chloroplast monothiol glutaredoxins as scaffold proteins for the assembly and delivery of [2Fe-2S] clusters
    Sibali Bandyopadhyay
    Department of Chemistry, Centre for Metalloenzyme Studies, University of Georgia, Athens, GA, USA
    EMBO J 27:1122-33. 2008
    ..These Grxs possess the same monothiol CGFS active site as yeast Grx5 and both were able to complement a yeast grx5 mutant defective in Fe-S cluster assembly...
  50. doi AtGRX4, an Arabidopsis chloroplastic monothiol glutaredoxin, is able to suppress yeast grx5 mutant phenotypes and respond to oxidative stress
    Ning Hui Cheng
    Plant Physiology Group, USDA ARS Children s Nutrition Research Center, Department of Pediatrics, Baylor College of Medicine, 1100 Bates Street, Houston, TX 77030, USA
    FEBS Lett 582:848-54. 2008
    ..monothiol glutaredoxin (Grx), AtGRX4, was targeted to chloroplasts/plastids and had high similarity to yeast Grx5. In yeast expression assays, AtGRX4 localized to the mitochondria and suppressed the sensitivity of grx5 cells to ..
  51. ncbi Cloning, functional analysis, and mitochondrial localization of Trypanosoma brucei monothiol glutaredoxin-1
    Michael Filser
    Biochemie Zentrum der Universitat Heidelberg, Im Neuenheimer Feld 504, D 69120 Heidelberg, Germany
    Biol Chem 389:21-32. 2008
    ..Expression of 1-C-Grx1 in Grx5-deficient yeast cells with its authentic presequence targeted the protein to the mitochondria and partially ..
  52. ncbi Redox properties and evolution of human glutaredoxins
    Johan Sagemark
    School of Life Sciences, Södertörns högskola, S 141 89 Huddinge, Sweden
    Proteins 68:879-92. 2007
    ..Three distinct groups were found: Grx1, Grx2, and Grx5, the latter representing a highly conserved group of monothiol glutaredoxins having a C-G-F-S active site sequence, ..
  53. ncbi The human counterpart of zebrafish shiraz shows sideroblastic-like microcytic anemia and iron overload
    Clara Camaschella
    Vita Salute University, Milan, Italy
    Blood 110:1353-8. 2007
    ..The zebrafish mutant shiraz has severe anemia and is embryonically lethal because of glutaredoxin 5 (GRLX5) deletion, insufficient biogenesis of mitochondrial iron-sulfur (Fe/S) clusters, and deregulated iron-..
  54. ncbi Effects of deleting mitochondrial antioxidant genes on life span
    Ercan Selcuk Unlu
    Department of Biology, Izmir Institute of Technology, 35430 Urla, Izmir, Turkey
    Ann N Y Acad Sci 1100:505-9. 2007
    ..it was found that among the known mitochondrial antioxidant genes (TTR1, CCD1, SOD1, GLO4, TRR2, TRX3, CCS1, SOD2, GRX5, PRX1), deletion of only three genes, SOD1 (Cu, Zn superoxide dismutase), SOD2 (Manganese-containing superoxide ..
  55. ncbi Monothiol glutaredoxins: a common domain for multiple functions
    E Herrero
    Departament de Ciencies Mediques Basiques, IRBLLEIDA, Universitat de Lleida, Montserrat Roig 2, Lleida, Spain
    Cell Mol Life Sci 64:1518-30. 2007
    ..Studies in Saccharomyces cerevisiae have demonstrated the role of the Grx5 protein in the biogenesis of iron-sulfur clusters...
  56. pmc In silico pathway reconstruction: Iron-sulfur cluster biogenesis in Saccharomyces cerevisiae
    Rui Alves
    Departament de Ciencies Mediques Basiques, Universidad de Lleida, Montserrat Roig 2, 25008 Lleida, Spain
    BMC Syst Biol 1:10. 2007
    ..One such class of pathways is involved in the biogenesis of Iron-Sulfur Clusters (ISC)...
  57. pmc Grx5 glutaredoxin plays a central role in protection against protein oxidative damage in Saccharomyces cerevisiae
    M T Rodríguez-Manzaneque
    Departament de Ciencies Mediques Basiques, Facultat de Medicina, Universitat de Lleida, 25198 Lleida, Spain
    Mol Cell Biol 19:8180-90. 1999
    ..In this work, another subfamily of yeast glutaredoxins (Grx3, Grx4, and Grx5) that differs from the first in containing a single cysteine residue at the putative active site is described...