GEMIN2

Summary

Gene Symbol: GEMIN2
Description: gem nuclear organelle associated protein 2
Alias: SIP1, SIP1-delta, gem-associated protein 2, SMN interacting protein 1-delta, component of gems 2, gemin-2, survival of motor neuron protein interacting protein 1
Species: human
Products:     GEMIN2

Top Publications

  1. Pellizzoni L, Baccon J, Charroux B, Dreyfuss G. The survival of motor neurons (SMN) protein interacts with the snoRNP proteins fibrillarin and GAR1. Curr Biol. 2001;11:1079-88 pubmed
    ..SMN and its associated proteins Gemin2, Gemin3, and Gemin4 form a large complex that plays a role in snRNP assembly, pre-mRNA splicing, and transcription...
  2. Ogawa C, Usui K, Aoki M, Ito F, Itoh M, Kai C, et al. Gemin2 plays an important role in stabilizing the survival of motor neuron complex. J Biol Chem. 2007;282:11122-34 pubmed
    ..Besides the SMN protein, Gemin2 is a core protein that is essential for the formation of the SMN complex, although the mechanism by which it ..
  3. Warrilow D, Tachedjian G, Harrich D. Maturation of the HIV reverse transcription complex: putting the jigsaw together. Rev Med Virol. 2009;19:324-37 pubmed publisher
    ..Determining precisely how these proteins assist the RTC function needs to be addressed. ..
  4. Nishitsuji H, Hayashi T, Takahashi T, Miyano M, Kannagi M, Masuda T. Augmentation of reverse transcription by integrase through an interaction with host factor, SIP1/Gemin2 Is critical for HIV-1 infection. PLoS ONE. 2009;4:e7825 pubmed publisher
    ..We previously identified a host factor, survival motor neuron-interacting protein 1 (SIP1/Gemin2) that binds to human immunodeficiency virus type 1 (HIV-1) IN and supports HIV-1 infection apparently at reverse ..
  5. Fischer U, Liu Q, Dreyfuss G. The SMN-SIP1 complex has an essential role in spliceosomal snRNP biogenesis. Cell. 1997;90:1023-9 pubmed
    ..The SMN protein is tightly associated with a novel protein, SIP1, and together they form a complex with several spliceosomal snRNP proteins...
  6. Zhang W, Wu J. Sip1, a novel RS domain-containing protein essential for pre-mRNA splicing. Mol Cell Biol. 1998;18:676-84 pubmed
    ..We report here identification and functional characterization of a new splicing factor, Sip1 (SC35-interacting protein 1)...
  7. Charroux B, Pellizzoni L, Perkinson R, Shevchenko A, Mann M, Dreyfuss G. Gemin3: A novel DEAD box protein that interacts with SMN, the spinal muscular atrophy gene product, and is a component of gems. J Cell Biol. 1999;147:1181-94 pubmed
    ..The SMN protein is part of a complex containing several proteins, of which one, SIP1 (SMN interacting protein 1), has been characterized so far...
  8. Charroux B, Pellizzoni L, Perkinson R, Yong J, Shevchenko A, Mann M, et al. Gemin4. A novel component of the SMN complex that is found in both gems and nucleoli. J Cell Biol. 2000;148:1177-86 pubmed
    ..In both compartments SMN is part of a large complex that contains several proteins including Gemin2 (formerly SIP1) and the DEAD box protein Gemin3...
  9. Young P, Man N, Lorson C, Le T, Androphy E, Burghes A, et al. The exon 2b region of the spinal muscular atrophy protein, SMN, is involved in self-association and SIP1 binding. Hum Mol Genet. 2000;9:2869-77 pubmed
    ..The presence of two separate self-association sites suggests a novel mechanism by which linear oligomers or closed rings might be formed from SMN monomers. ..

More Information

Publications122 found, 100 shown here

  1. Selenko P, Sprangers R, Stier G, Buhler D, Fischer U, Sattler M. SMN tudor domain structure and its interaction with the Sm proteins. Nat Struct Biol. 2001;8:27-31 pubmed
    ..Our data provide a structural basis for a molecular defect underlying SMA. ..
  2. Gubitz A, Mourelatos Z, Abel L, Rappsilber J, Mann M, Dreyfuss G. Gemin5, a novel WD repeat protein component of the SMN complex that binds Sm proteins. J Biol Chem. 2002;277:5631-6 pubmed
    ..SMN is part of a multi-protein complex that includes Gemin2, Gemin3, and Gemin4...
  3. Chari A, Golas M, Klingenhäger M, Neuenkirchen N, Sander B, Englbrecht C, et al. An assembly chaperone collaborates with the SMN complex to generate spliceosomal SnRNPs. Cell. 2008;135:497-509 pubmed publisher
    ..The mode of action of this combined chaperone/catalyst system is reminiscent of the mechanism employed by DNA clamp loaders. ..
  4. Yong J, Kasim M, Bachorik J, Wan L, Dreyfuss G. Gemin5 delivers snRNA precursors to the SMN complex for snRNP biogenesis. Mol Cell. 2010;38:551-62 pubmed publisher
    ..We propose a stepwise pathway of SMN complex formation and snRNP biogenesis, highlighting Gemin5's function in delivering pre-snRNAs as substrates for Sm core assembly and processing. ..
  5. Zhang R, So B, Li P, Yong J, Glisovic T, Wan L, et al. Structure of a key intermediate of the SMN complex reveals Gemin2's crucial function in snRNP assembly. Cell. 2011;146:384-95 pubmed publisher
    ..Specific Sm core assembly depends on Sm proteins and snRNA recognition by SMN/Gemin2- and Gemin5-containing subunits, respectively...
  6. Liu Q, Fischer U, Wang F, Dreyfuss G. The spinal muscular atrophy disease gene product, SMN, and its associated protein SIP1 are in a complex with spliceosomal snRNP proteins. Cell. 1997;90:1013-21 pubmed
    ..The function of the SMN protein is unknown. We found that SMN is tightly associated with a novel protein, SIP1, and together they form a specific complex with several spliceosomal snRNP proteins...
  7. Pellizzoni L, Charroux B, Rappsilber J, Mann M, Dreyfuss G. A functional interaction between the survival motor neuron complex and RNA polymerase II. J Cell Biol. 2001;152:75-85 pubmed
    ..These findings indicate a functional as well as physical association of the SMN complex with pol II and suggest a role for the SMN complex in the assembly of the pol II transcription/processing machinery. ..
  8. Jablonka S, Bandilla M, Wiese S, Buhler D, Wirth B, Sendtner M, et al. Co-regulation of survival of motor neuron (SMN) protein and its interactor SIP1 during development and in spinal muscular atrophy. Hum Mol Genet. 2001;10:497-505 pubmed
    ..In a first attempt to address this issue we have investigated the Smn interacting protein 1 (Sip1), with an emphasis on its developmental expression and subcellular distribution in spinal motor neurons in relation ..
  9. Meister G, Buhler D, Laggerbauer B, Zobawa M, Lottspeich F, Fischer U. Characterization of a nuclear 20S complex containing the survival of motor neurons (SMN) protein and a specific subset of spliceosomal Sm proteins. Hum Mol Genet. 2000;9:1977-86 pubmed
    ..This shows that the SMN-Sm protein interaction is not restricted to the cytoplasm. Our data imply that nuclear SMN affects splicing by modulating the Sm protein composition of U snRNPs. ..
  10. Pellizzoni L, Baccon J, Rappsilber J, Mann M, Dreyfuss G. Purification of native survival of motor neurons complexes and identification of Gemin6 as a novel component. J Biol Chem. 2002;277:7540-5 pubmed
    ..The SMN complex is localized in both the cytoplasm and the nucleus and contains SMN, Gemin2, Gemin3, Gemin4, Gemin5, and a few not yet identified proteins...
  11. Wan L, Battle D, Yong J, Gubitz A, Kolb S, Wang J, et al. The survival of motor neurons protein determines the capacity for snRNP assembly: biochemical deficiency in spinal muscular atrophy. Mol Cell Biol. 2005;25:5543-51 pubmed
    ..Thus, SMN determines the capacity for snRNP biogenesis, and our findings provide evidence for a measurable deficiency in a biochemical activity in cells from patients with spinal muscular atrophy. ..
  12. Sleeman J, Lamond A. Newly assembled snRNPs associate with coiled bodies before speckles, suggesting a nuclear snRNP maturation pathway. Curr Biol. 1999;9:1065-74 pubmed
    ..These include speckles, coiled bodies and nucleoli, in addition to a diffuse nucleoplasmic compartment. The reasons for snRNP accumulation in these different structures are unclear...
  13. Hamamoto S, Nishitsuji H, Amagasa T, Kannagi M, Masuda T. Identification of a novel human immunodeficiency virus type 1 integrase interactor, Gemin2, that facilitates efficient viral cDNA synthesis in vivo. J Virol. 2006;80:5670-7 pubmed
    ..Here, using a yeast two-hybrid system, we identified a survival motor neuron (SMN)-interacting protein 1 (Gemin2) that binds to HIV-1 IN...
  14. Takizawa Y, Qing Y, Takaku M, Ishida T, Morozumi Y, Tsujita T, et al. GEMIN2 promotes accumulation of RAD51 at double-strand breaks in homologous recombination. Nucleic Acids Res. 2010;38:5059-74 pubmed publisher
    ..We found that human RAD51 directly binds GEMIN2/SIP1, a protein involved in spliceosome biogenesis...
  15. Lacher M, Shiina M, Chang P, Keller D, Tiirikainen M, Korn W. ZEB1 limits adenoviral infectability by transcriptionally repressing the coxsackie virus and adenovirus receptor. Mol Cancer. 2011;10:91 pubmed publisher
    ..associated with transcriptional repression of E-cadherin by, for instance, the E2 box-binding factors Snail, Slug, SIP1 or ZEB1...
  16. Zu C, Liu S, Cao W, Liu Z, Qiang H, Li Y, et al. MiR-590-3p suppresses epithelial-mesenchymal transition in intrahepatic cholangiocarcinoma by inhibiting SIP1 expression. Oncotarget. 2017;8:34698-34708 pubmed publisher
    ..MiR-590-3p dramatically suppressed epithelial-mesenchymal transition, cell migration, and invasion of ICC cells. SIP1 was identified as direct and functional target of miR-590-3p in ICC cells by luciferase assays...
  17. Saydam O, Shen Y, Wurdinger T, Senol O, Boke E, James M, et al. Downregulated microRNA-200a in meningiomas promotes tumor growth by reducing E-cadherin and activating the Wnt/beta-catenin signaling pathway. Mol Cell Biol. 2009;29:5923-40 pubmed publisher
    ..Upregulation of miR-200a decreased the expression of transcription factors ZEB1 and SIP1, with consequent increased expression of E-cadherin, an adhesion protein associated with cell differentiation...
  18. Meier I, Walker M, Matera A. Gemin4 is an essential gene in mice, and its overexpression in human cells causes relocalization of the SMN complex to the nucleoplasm. Biol Open. 2018;7: pubmed publisher
    ..Overexpression of Gemin4 constructs lacking this NLS sequestered Gemin3 and, to a lesser extent Gemin2, in the cytoplasm but had little effect on the nuclear accumulation of SMN...
  19. Xu H, Shi X, He L, Guo Y, Zang D, Li H, et al. Arabidopsis thaliana Trihelix Transcription Factor AST1 Mediates Salt and Osmotic Stress Tolerance by Binding to a Novel AGAG-Box and Some GT Motifs. Plant Cell Physiol. 2018;59:946-965 pubmed publisher
    ..In the present study, we characterized the function of AST1 (Arabidopsis SIP1 clade Trihelix1) in response to salt and osmotic stress...
  20. Borg R, Bordonne R, Vassallo N, Cauchi R. Genetic Interactions between the Members of the SMN-Gemins Complex in Drosophila. PLoS ONE. 2015;10:e0130974 pubmed publisher
    ..and motor phenotypes associated with a hypomorphic Gemin3 mutant are enhanced by changes in the levels of SMN, Gemin2 and Gemin5 brought about by various genetic manipulations...
  21. Okamoto Y, Nishimura N, Matsuda K, Ranawakage D, Kamachi Y, Kondoh H, et al. Cooperation of Sall4 and Sox8 transcription factors in the regulation of the chicken Sox3 gene during otic placode development. Dev Growth Differ. 2018;60:133-145 pubmed publisher
    ..The CAGGTG sequence likely serves as the binding sites of the repressor TFs ?EF1 (Zeb1), Sip1 (Zeb2), and Snail2, all of which are expressed in the cephalic neural crest but not in the otic placode...
  22. Turovskaya M, Zinchenko V, Babaev A, Epifanova E, Tarabykin V, Turovsky E. Mutation in the Sip1 transcription factor leads to a disturbance of the preconditioning of AMPA receptors by episodes of hypoxia in neurons of the cerebral cortex due to changes in their activity and subunit composition. The protective effects of int. Arch Biochem Biophys. 2018;654:126-135 pubmed publisher
    The Sip1 mutation plays the main role in pathogenesis of the Mowat-Wilson syndrome, which is characterized by the pronounced epileptic symptoms...
  23. Matera A, Raimer A, Schmidt C, Kelly J, Droby G, Baillat D, et al. Composition of the Survival Motor Neuron (SMN) Complex in Drosophila melanogaster. G3 (Bethesda). 2019;9:491-503 pubmed publisher
    ..Using transgenic flies that exclusively express Flag-tagged SMN from its native promoter, we previously found that Gemin2, Gemin3, Gemin5, and all nine classical Sm proteins, including Lsm10 and Lsm11, co-purify with SMN...
  24. Xu G, Zhang L, Ma A, Qian Y, Ding Q, Liu Y, et al. SIP1 is a downstream effector of GADD45G in senescence induction and growth inhibition of liver tumor cells. Oncotarget. 2015;6:33636-47 pubmed publisher
    ..Here, we report that the Smad-interacting protein-1 (SIP1) is transcriptionally activated and functions critically in the GADD45G-induced tumor cell senescence...
  25. Fukagawa A, Ishii H, Miyazawa K, Saitoh M. δEF1 associates with DNMT1 and maintains DNA methylation of the E-cadherin promoter in breast cancer cells. Cancer Med. 2015;4:125-35 pubmed publisher
    ..EF1 family proteins (δEF1/ZEB1 and SIP1/ZEB2), key regulators of the epithelial-mesenchymal transition (EMT), suppress E-cadherin expression at the ..
  26. Sun X, Sun Z, Zhu Z, Li C, Zhang J, Xu H, et al. Expression of SIP1 is strongly correlated with LDHA and shows a significantly poor outcome in gastric cancer. Tumour Biol. 2015;36:7521-30 pubmed publisher
    Smad interacting protein 1 (SIP1) plays an important role in the epithelial-mesenchymal transition (EMT) process by downregulating E-cadherin...
  27. Bulzico D, Torres D, Ferreira G, Pires B, de Faria P, Hassan R, et al. A Novel TP53 Mutation Associated with TWIST1 and SIP1 Expression in an Aggressive Adrenocortical Carcinoma. Endocr Pathol. 2017;28:326-331 pubmed publisher
    ..Epithelial-mesenchymal transition (EMT) transcription factors TWIST1 and Smad interacting protein 1 (SIP1) are related to poorer outcomes in other malignancies, but their role in ACC is unknown...
  28. Volk D, Edelson J, Lewis D. Altered expression of developmental regulators of parvalbumin and somatostatin neurons in the prefrontal cortex in schizophrenia. Schizophr Res. 2016;177:3-9 pubmed publisher
    ..Finally, expression levels of other important developmental regulators (i.e. Dlx1, Dlx5, Dlx6, SATB1, Sip1/Zeb2, ST8SIA4, cMaf, Nkx6.2, and Arx) were not altered in schizophrenia...
  29. Sathyanarayanan A, Chandrasekaran K, Karunagaran D. microRNA-145 downregulates SIP1-expression but differentially regulates proliferation, migration, invasion and Wnt signaling in SW480 and SW620 cells. J Cell Biochem. 2018;119:2022-2035 pubmed publisher
    ..In SW480 and SW620 cells, SMAD-interacting protein 1 (SIP1), was identified as a target of miR-145, and its expression was suppressed both at mRNA and protein levels, and ..
  30. Wang Z, Zhao K, Pan Y, Wang J, Song X, Ge W, et al. Genomic, expressional, protein-protein interactional analysis of Trihelix transcription factor genes in Setaria italia and inference of their evolutionary trajectory. BMC Genomics. 2018;19:665 pubmed publisher
    ..Six amino acid sites of the SIP1 subfamily possibly were likely subjected to significant positive selection...
  31. Jouppila Mättö A, Mannermaa A, Sironen R, Kosma V, Soini Y, Pukkila M. SIP1 predicts progression and poor prognosis in pharyngeal squamous cell carcinoma. Histol Histopathol. 2015;30:569-79 pubmed publisher
    ..The transcription factors SIP1, SLUG, ZEB1, SNAI1, and TWIST are fundamental in regulating EMT...
  32. Yoshida R, Morita M, Shoji F, Nakashima Y, Miura N, Yoshinaga K, et al. Clinical Significance of SIP1 and E-cadherin in Patients with Esophageal Squamous Cell Carcinoma. Ann Surg Oncol. 2015;22:2608-14 pubmed publisher
    ..Smad interacting protein 1 (SIP1) is an EMT trigger, which is inversely correlated with E-cadherin in some carcinomas...
  33. Takagi T, Nishizaki Y, Matsui F, Wakamatsu N, Higashi Y. De novo inbred heterozygous Zeb2/Sip1 mutant mice uniquely generated by germ-line conditional knockout exhibit craniofacial, callosal and behavioral defects associated with Mowat-Wilson syndrome. Hum Mol Genet. 2015;24:6390-402 pubmed publisher
    Mowat-Wilson syndrome (MOWS) is caused by de novo heterozygous mutation at ZEB2 (SIP1, ZFHX1B) gene, and exhibit moderate to severe intellectual disability (ID), a characteristic facial appearance, epilepsy and other congenital anomalies...
  34. Wu Y, Chung I, Wong W, Masamune A, Sim M, Looi C. Paracrine IL-6 signaling mediates the effects of pancreatic stellate cells on epithelial-mesenchymal transition via Stat3/Nrf2 pathway in pancreatic cancer cells. Biochim Biophys Acta Gen Subj. 2017;1861:296-306 pubmed publisher
    ..and invasion capacity, and mesenchymal-like gene expression of N-cadherin, vimentin, fibronectin, collagen I, Sip1, Snail, Slug, and Twist2. Inhibition of JAK/Stat3 signaling induced by IL-6 repressed EMT and Nrf2 gene expression...
  35. Serrano M, Liu J, Svoboda K, Nawshad A, Benson M. Ephrin reverse signaling mediates palatal fusion and epithelial-to-mesenchymal transition independently of Tgfß3. J Cell Physiol. 2015;230:2961-72 pubmed publisher
    ..They concurrently increased their expression of the EMT-associated transcription factors Snail, Sip1, and Twist1. EphB2/Fc did not cause apoptosis in these cells...
  36. Abeysundara N, Leung A, Primrose D, Hughes S. Regulation of cell proliferation and adhesion by means of a novel region of drosophila merlin interacting with Sip1. Dev Dyn. 2014;243:1554-70 pubmed publisher
    ..We investigate how the Drosophila NHERF1 orthologue, Sip1, and Merlin function to regulate cell proliferation and adhesion...
  37. Chen D, Liu G, Xu N, You X, Zhou H, Zhao X, et al. Knockdown of ARK5 Expression Suppresses Invasion and Metastasis of Gastric Cancer. Cell Physiol Biochem. 2017;42:1025-1036 pubmed publisher
    ..And knockdown of ARK5 expression in GC cells resulted in the down-regulation of the mTOR/p70S6k signals, Slug and SIP1. The elevated ARK5 expression was closely associated with cancer metastasis and patient survival, and it seemed to ..
  38. Jin W, Wang Y, Liu C, Yang N, Jin M, Cong Y, et al. Structural basis for snRNA recognition by the double-WD40 repeat domain of Gemin5. Genes Dev. 2016;30:2391-2403 pubmed
    ..The SMN and Gemin2 subunits directly bind Sm proteins, and Gemin5 is required for snRNP biogenesis and has been implicated in snRNA ..
  39. Gao H, Li X, Zhang Z, Wang W, Huang Y. [Coding-sequence point mutation and polymorphism analyses of SIP1 gene in Hirschsprung disease]. Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2008;25:82-5 pubmed
    To investigate the point mutations and polymorphisms of SIP1 gene in Hirschsprung disease(HSCR) and discuss the relationship between the feature of gene mutations and single nucleotide polymorphisms of SIP1 gene and HSCR...
  40. Hoshi Y, Endo K, Shirakihara T, Fukagawa A, Miyazawa K, Saitoh M. The potential role of regulator of G-protein signaling 16 in cell motility mediated by δEF1 family proteins. FEBS Lett. 2016;590:270-8 pubmed publisher
    ..We reported previously that expression of the δEF1 family proteins (δEF1/ZEB1 and SIP1/ZEB2), key regulators of the EMT, is positively correlated with EMT phenotypes and aggressiveness of breast cancer...
  41. Turovskaya M, Babaev A, Zinchenko V, Epifanova E, Borisova E, Tarabykin V, et al. Sip-1 mutations cause disturbances in the activity of NMDA- and AMPA-, but not kainate receptors of neurons in the cerebral cortex. Neurosci Lett. 2017;650:180-186 pubmed publisher
    Smad-interacting protein-1 (Sip1) [Zinc finger homeobox (Zfhx1b), Zeb2] is a transcription factor implicated in the genesis of Mowat-Wilson syndrome (MWS) in humans...
  42. Schlaen R, Mancini E, Sanchez S, Perez Santángelo S, Rugnone M, Simpson C, et al. The spliceosome assembly factor GEMIN2 attenuates the effects of temperature on alternative splicing and circadian rhythms. Proc Natl Acad Sci U S A. 2015;112:9382-7 pubmed publisher
    ..Here we show that GEMIN2, a spliceosomal small nuclear ribonucleoprotein assembly factor conserved from yeast to humans, modulates low ..
  43. Bhardwaj M, Sen S, Sharma A, Kashyap S, Chosdol K, Pushker N, et al. ZEB2/SIP1 as novel prognostic indicator in eyelid sebaceous gland carcinoma. Hum Pathol. 2015;46:1437-42 pubmed publisher
    ..ZEB2/SIP1 is an important EMT regulator and down-regulates E-cadherin expression...
  44. Quintes S, Brinkmann B, Ebert M, Fröb F, Kungl T, Arlt F, et al. Zeb2 is essential for Schwann cell differentiation, myelination and nerve repair. Nat Neurosci. 2016;19:1050-1059 pubmed publisher
    ..Here we show that transcriptional repression, mediated by the zinc-finger protein Zeb2 (also known as Sip1), is essential for differentiation and myelination...
  45. Galindo Hernandez O, Serna Marquez N, Castillo Sanchez R, Salazar E. Extracellular vesicles from MDA-MB-231 breast cancer cells stimulated with linoleic acid promote an EMT-like process in MCF10A cells. Prostaglandins Leukot Essent Fatty Acids. 2014;91:299-310 pubmed publisher
    ..LA induce a transient down-regulation of E-cadherin expression, and an increase of Snail1 and 2, Twist1 and 2, Sip1, vimentin and N-cadherin expression in MCF10A cells...
  46. Qi X, Ng K, Lian Q, Liu X, Li C, Geng W, et al. Clinical significance and therapeutic value of glutathione peroxidase 3 (GPx3) in hepatocellular carcinoma. Oncotarget. 2014;5:11103-20 pubmed
    ..Tumor suppressive activity of GPx3 was mediated through Erk-NFκB-SIP1 pathway. GPx3 could be delivered by hiPSC-MSCs into the tumor and exhibited tumor suppressive activity in vivo...
  47. Zhou Z, Sun B, Sun L. Edwardsiella tarda Sip1: A serum-induced zinc metalloprotease that is essential to serum resistance and host infection. Vet Microbiol. 2015;177:332-40 pubmed publisher
    ..Five differentially expressed proteins were identified, one of which was a putative zinc protease (named Sip1). Western blot confirmed extracellular production of Sip1 by E. tarda...
  48. Huo H, Tian J, Wang R, Li Y, Qu C, Wang N. Long non-coding RNA NORAD upregulate SIP1 expression to promote cell proliferation and invasion in cervical cancer. Biomed Pharmacother. 2018;106:1454-1460 pubmed publisher
    ..that lncRNA NORAD could sponge miR-590-3p to promote the proliferation and invasion of CC cells via upregulating SIP1 expression. In addition, we showed that NORAD inhibition could reduce CC cells growth in vivo...
  49. Li A, Omura N, Hong S, Vincent A, Walter K, Griffith M, et al. Pancreatic cancers epigenetically silence SIP1 and hypomethylate and overexpress miR-200a/200b in association with elevated circulating miR-200a and miR-200b levels. Cancer Res. 2010;70:5226-37 pubmed publisher
    ..We also identified prevalent hypermethylation and silencing of one of their downstream targets, SIP1 (ZFHX1B, ZEB2), whose protein product suppresses E-cadherin expression and contributes to epithelial mesenchymal ..
  50. Xiao L, Zhou X, Liu F, Hu C, Zhu X, Luo Y, et al. MicroRNA-129-5p modulates epithelial-to-mesenchymal transition by targeting SIP1 and SOX4 during peritoneal dialysis. Lab Invest. 2015;95:817-832 pubmed publisher
    ..cell line (HPMC), treated with TGF-β1, whereas expression of vimentin, fibronectin and transcription factors SIP1 and SOX4 increased significantly, as assessed by real-time PCR, western blot analysis and immunofluorescence ..
  51. Fleckenstein T, Kastenmüller A, Stein M, Peters C, Daake M, Krause M, et al. The Chaperone Activity of the Developmental Small Heat Shock Protein Sip1 Is Regulated by pH-Dependent Conformational Changes. Mol Cell. 2015;58:1067-78 pubmed publisher
    ..In Caenorhabditis elegans, Sip1 is the only sHsp exclusively expressed in oocytes and embryos...
  52. He L, Yu K, Lu F, Wang J, Wu L, Zhao C, et al. Transcriptional Regulator ZEB2 Is Essential for Bergmann Glia Development. J Neurosci. 2018;38:1575-1587 pubmed publisher
    ..In this report, we show that ZEB2 (aka, SIP1 or ZFHX1B), a Mowat-Wilson syndrome-associated transcriptional regulator, is highly expressed in Bergmann glia, but ..
  53. Liu Y, Siles L, Lu X, Dean K, Cuatrecasas M, Postigo A, et al. Mitotic polarization of transcription factors during asymmetric division establishes fate of forming cancer cells. Nat Commun. 2018;9:2424 pubmed publisher
    ..In these cells, Zeb1 represses the Smad inhibitor Zeb2/Sip1, causing Pten loss and launching Tgf-β1 signaling that drives nuclear translocation of Yap1...
  54. Wang C, Chen Q, Xiang N, Liu Y, Kong X, Yang Y, et al. SIP1, a novel SOS2 interaction protein, is involved in salt-stress tolerance in Arabidopsis. Plant Physiol Biochem. 2018;124:167-174 pubmed publisher
    ..protein was identified by yeast two hybrid (Y2H) library and was referred to as SOS2 interaction protein 1 (SIP1)...
  55. Yu C, Cai X, Ye Z, Li H. Genome-wide identification and expression profiling analysis of trihelix gene family in tomato. Biochem Biophys Res Commun. 2015;468:653-9 pubmed publisher
    ..SlGT members can be classified into six subgroups (GT-1, GT-2, SH4, SIP1, GT-γ and GT-δ)...
  56. Yang L, Wang Y, Shi Y, Bu H, Ye F. Deletion of SIP1 promotes liver regeneration and lipid accumulation. Pathol Res Pract. 2016;212:421-5 pubmed publisher
    The function of Smad interacting protein-1 (SIP1) in liver regeneration is not yet known...
  57. Bulzico D, Faria P, Maia C, de Paula M, Torres D, Ferreira G, et al. Is there a role for epithelial-mesenchymal transition in adrenocortical tumors?. Endocrine. 2017;58:276-288 pubmed publisher
    ..of EMT markers e-cadherin, vimentin, and fibronectin, along with EMT-transcription factors (EMT-TFs), TWIST1, SIP1, and SNAIL in 24 adrenocortical carcinoma (ACC), 19 adrenocortical adenomas (ACA), 27 childhood-onset ..
  58. Oztas E, Avci M, Ozcan A, Sayan A, Tulchinsky E, Yagci T. Novel monoclonal antibodies detect Smad-interacting protein 1 (SIP1) in the cytoplasm of human cells from multiple tumor tissue arrays. Exp Mol Pathol. 2010;89:182-9 pubmed publisher
    Smad-interacting protein 1 (SIP1, also known as ZEB2) represses the transcription of E-cadherin and mediates epithelial-mesenchymal transition in development and tumor metastasis...
  59. Kiliç E, Cetinkaya A, Utine G, BoduroÄŸlu K. A Diagnosis to Consider in Intellectual Disability: Mowat-Wilson Syndrome. J Child Neurol. 2016;31:913-7 pubmed publisher
    ..The condition is caused by de novo heterozygous mutations or deletions in ZEB2 gene located at 2q22. ZEB2 encodes Sip1 protein, which acts during central nervous system development as an important transcription factor...
  60. So B, Wan L, Zhang Z, Li P, Babiash E, Duan J, et al. A U1 snRNP-specific assembly pathway reveals the SMN complex as a versatile hub for RNP exchange. Nat Struct Mol Biol. 2016;23:225-30 pubmed publisher
    ..by the SMN complex, the snRNA-specific RNA-binding protein (RBP) Gemin5 delivers pre-snRNAs, which join SMN-Gemin2-recruited Sm proteins...
  61. Tseng Y, Chen C, Jong Y, Chang F, Lo Y. Loganin possesses neuroprotective properties, restores SMN protein and activates protein synthesis positive regulator Akt/mTOR in experimental models of spinal muscular atrophy. Pharmacol Res. 2016;111:58-75 pubmed publisher
    ..In SMN-deficient NSC34 cells, loganin increased cell viability, neurite length, and expressions of SMN, Gemin2, SMN-Gemin2 complex, p-Akt, p-GSK-3β, p-CREB, BDNF and Bcl-2...
  62. Borg R, Fenech Salerno B, Vassallo N, Bordonne R, Cauchi R. Disruption of snRNP biogenesis factors Tgs1 and pICln induces phenotypes that mirror aspects of SMN-Gemins complex perturbation in Drosophila, providing new insights into spinal muscular atrophy. Neurobiol Dis. 2016;94:245-58 pubmed publisher
    ..overexpression of both factors leads to motor dysfunction in Drosophila, a situation analogous to that of Gemin2. Toxicity is conserved in the yeast S...
  63. Sanz P, Viana R, Garcia Gimeno M. AMPK in Yeast: The SNF1 (Sucrose Non-fermenting 1) Protein Kinase Complex. Exp Suppl. 2016;107:353-374 pubmed
    ..orthologue of AMPKα catalytic subunit), Snf4 (orthologue of AMPKγ regulatory subunit), and a member of the Gal83/Sip1/Sip2 family of proteins (orthologues of AMPKβ subunit) that act as scaffolds and also regulate the subcellular ..
  64. Kanda M, Shimizu D, Fujii T, Tanaka H, Shibata M, Iwata N, et al. Protein arginine methyltransferase 5 is associated with malignant phenotype and peritoneal metastasis in gastric cancer. Int J Oncol. 2016;49:1195-202 pubmed publisher
    ..Differential expression of PRMT5 mRNA by GC cell lines correlated positively with the levels of GEMIN2, STAT3 and TGFB3. PRMT5 knockdown reduced the proliferation, invasion and migration of a GC cell line...
  65. Sathyanarayanan A, Chandrasekaran K, Karunagaran D. microRNA-145 modulates epithelial-mesenchymal transition and suppresses proliferation, migration and invasion by targeting SIP1 in human cervical cancer cells. Cell Oncol (Dordr). 2017;40:119-131 pubmed publisher
    ..The identification of the SMAD-interacting protein 1 (SIP1) mRNA as putative miR-145 target was investigated using a 3' untranslated region (3'UTR) luciferase assay and ..
  66. Tang Y, Yan G, Song X, Wu K, Li Z, Yang C, et al. STIP overexpression confers oncogenic potential to human non-small cell lung cancer cells by regulating cell cycle and apoptosis. J Cell Mol Med. 2015;19:2806-17 pubmed publisher
    b>Sip1/tuftelin-interacting protein (STIP), a multidomain nuclear protein, is a novel factor associated with the spliceosome, yet its role and molecular function in cancer remain unknown...
  67. Sun S, Wang Y, Chen H, Fang L, Cui Y, Han X, et al. STIP Regulates ERK1/2 Signaling Pathway Involved in Interaction with PP1? in Lymphoblastic Leukemia. Curr Mol Med. 2016;16:767-775 pubmed
    b>Sip1/Tuftelin Interacting Protein (STIP) is highly conserved from Caenorhabditis elegans to Homo sapiens and has essential biological functions. However, its function in leukemia remains unknown...
  68. Jääskeläinen A, Soini Y, Jukkola Vuorinen A, Auvinen P, Haapasaari K, Karihtala P. High-level cytoplasmic claudin 3 expression is an independent predictor of poor survival in triple-negative breast cancer. BMC Cancer. 2018;18:223 pubmed publisher
    ..The expression of epithelial-to-mesenchymal transition-regulating transcription factors Sip1, Zeb1 and vimentin had previously been determined in the same material...
  69. Friesen W, Massenet S, Paushkin S, Wyce A, Dreyfuss G. SMN, the product of the spinal muscular atrophy gene, binds preferentially to dimethylarginine-containing protein targets. Mol Cell. 2001;7:1111-7 pubmed
    ..It is reasonable to expect that protein hypomethylation may contribute to the severity of SMA. ..
  70. Pires B, Mencalha A, Ferreira G, de Souza W, Morgado Diaz J, Maia A, et al. NF-kappaB Is Involved in the Regulation of EMT Genes in Breast Cancer Cells. PLoS ONE. 2017;12:e0169622 pubmed publisher
    ..related to the expression of the epithelial-to-mesenchymal transition transcription factors (EMT-TFs) SNAIL, SLUG, SIP1 and TWIST1...
  71. Kimura Y, Irie K, Mizuno T. Expression control of the AMPK regulatory subunit and its functional significance in yeast ER stress response. Sci Rep. 2017;7:46713 pubmed publisher
    ..However, it remains unclear how the alternate three ? subunits, Sip1, Sip2, and Gal83, of the Snf1 complex participate in ER stress response...
  72. Zhao W, Zhang S, Huang W, Li X. Mutations in Smad-interacting protein 1 gene are responsible for absence of its expression in Hirschsprung's disease. Clin Exp Med. 2018;18:445-451 pubmed publisher
    ..The pathophysiological basis remains unclear. Recently, the SIP1 gene has been recognized as being involved in the pathogenesis of symptomatic HSCR patients with 2q22 chromosomal ..
  73. Li W. How do SMA-linked mutations of SMN1 lead to structural/functional deficiency of the SMA protein?. PLoS ONE. 2017;12:e0178519 pubmed publisher
    ..From the computational analysis, it is also possible that SMN's Lys45 and Asp36 act as two electrostatic clips at the SMN-Gemin2 complex structure interface.
  74. Park J, Voss P, Grabski S, Wang J, Patterson R. Association of galectin-1 and galectin-3 with Gemin4 in complexes containing the SMN protein. Nucleic Acids Res. 2001;29:3595-602 pubmed
    ..from HeLa cell nuclear extracts, along with galectin-1, polypeptides identified to be in this complex: SMN, Gemin2 and the Sm polypeptides of snRNPs...
  75. Darr A, Danzi M, Brady L, Emig Agius D, Hackett A, Golshani R, et al. Identification of genome-wide targets of Olig2 in the adult mouse spinal cord using ChIP-Seq. PLoS ONE. 2017;12:e0186091 pubmed publisher
    ..2, Nkx6.2, and Sip1), myelination and ensheathment (Mbp, Cldn11, and Mobp), as well as cell cycle and cytoskeletal regulation...
  76. Wang Z, Wu X, Friedberg E. Molecular mechanism of base excision repair of uracil-containing DNA in yeast cell-free extracts. J Biol Chem. 1997;272:24064-71 pubmed
    ..The processing of the 5'-deoxyribose phosphate moieties was found to be a rate-limiting step. During BER of uracil-containing DNA, repair patch sizes of 1-5 nucleotides were detected, with single nucleotide repair patches predominant. ..
  77. Wang J, Dreyfuss G. A cell system with targeted disruption of the SMN gene: functional conservation of the SMN protein and dependence of Gemin2 on SMN. J Biol Chem. 2001;276:9599-605 pubmed
    ..Interestingly, the level of the SMN-interacting protein Gemin2 decreases significantly following depletion of SMN, supporting the conclusion that SMN and Gemin2 form a stable ..
  78. Friesen W, Paushkin S, Wyce A, Massenet S, Pesiridis G, Van Duyne G, et al. The methylosome, a 20S complex containing JBP1 and pICln, produces dimethylarginine-modified Sm proteins. Mol Cell Biol. 2001;21:8289-300 pubmed
  79. Malatesta M, Scassellati C, Meister G, Plöttner O, Bühler D, Sowa G, et al. Ultrastructural characterisation of a nuclear domain highly enriched in survival of motor neuron (SMN) protein. Exp Cell Res. 2004;292:312-21 pubmed
    ..corresponding to gems, we have investigated the intranuclear localisation of SMN and of its interacting protein Gemin2 by immunoelectron microscopy in cultured cells and in liver cells of hibernating dormouse...
  80. Hu M, Xia M, Chen X, Lin Z, Xu Y, Ma Y, et al. MicroRNA-141 regulates Smad interacting protein 1 (SIP1) and inhibits migration and invasion of colorectal cancer cells. Dig Dis Sci. 2010;55:2365-72 pubmed publisher
    ..Expression of miR-141 and Smad interacting protein 1 (SIP1) were detected by real-time reverse-transcription polymerase chain reaction (RT-PCR) and Western blotting...
  81. Warren K, Warrilow D, Meredith L, Harrich D. Reverse Transcriptase and Cellular Factors: Regulators of HIV-1 Reverse Transcription. Viruses. 2009;1:873-94 pubmed publisher
    ..is an RT binding protein and a number of IN-binding proteins including INI1, components of the Sin3a complex, and Gemin2 affect reverse transcription...
  82. Martin R, Gupta K, Ninan N, Perry K, Van Duyne G. The survival motor neuron protein forms soluble glycine zipper oligomers. Structure. 2012;20:1929-39 pubmed publisher
    ..These results have important implications for advancing our understanding of SMN function and glycine zipper-mediated helix-helix interactions. ..
  83. Piazzon N, Schlotter F, Lefebvre S, Dodré M, Mereau A, Soret J, et al. Implication of the SMN complex in the biogenesis and steady state level of the signal recognition particle. Nucleic Acids Res. 2013;41:1255-72 pubmed publisher
    ..Additionally, microinjected antibodies directed against SMN or Gemin2 interfere with the association of SRP54 with 7S RNA in Xenopus laevis oocytes...
  84. Schwer B, Roth A, Shuman S. Will the circle be unbroken: specific mutations in the yeast Sm protein ring expose a requirement for assembly factor Brr1, a homolog of Gemin2. RNA. 2017;23:420-430 pubmed publisher
    ..the function of the yeast Sm assembly factor Brr1 and its relationship to the metazoan Sm assembly factor Gemin2. Mutations in the adjacent SmE (K83A), SmF (K32A, F33A, R74K), SmD2 (R49A, ..
  85. Lutz G, Jurak I, Kim E, Kim J, Hackenberg M, Leader A, et al. Viral Ubiquitin Ligase Stimulates Selective Host MicroRNA Expression by Targeting ZEB Transcriptional Repressors. Viruses. 2017;9: pubmed publisher
    ..enhancer binding factor 1 (δEF1) and zinc finger E-box binding homeobox 2 (ZEB2)/Smad-interacting protein 1 (SIP1), which we establish as new substrates for ICP0-mediated degradation...
  86. Zhao Y, Sun Y, Huang A, Li X, Huang C, Ma T, et al. MicroRNA-200a induces apoptosis by targeting ZEB2 in alcoholic liver disease. Cell Cycle. 2017;:1-32 pubmed publisher
    ..examined it's effect on cell apoptosis and identified zinc finger E-box binding homeobox 2 (ZEB2; also known as SIP1) as a direct target gene of miR-200a...
  87. Helmken C, Wetter A, Rudnik Schoneborn S, Liehr T, Zerres K, Wirth B. An essential SMN interacting protein (SIP1) is not involved in the phenotypic variability of spinal muscular atrophy (SMA). Eur J Hum Genet. 2000;8:493-9 pubmed
    The survival motor neuron (SMN) protein and the SMN interacting protein 1 (SIP1) are part of a 300 kD protein complex with a crucial role in snRNP biogenesis and pre-mRNA splicing...
  88. Carnegie G, Sleeman J, Morrice N, Hastie C, Peggie M, Philp A, et al. Protein phosphatase 4 interacts with the Survival of Motor Neurons complex and enhances the temporal localisation of snRNPs. J Cell Sci. 2003;116:1905-13 pubmed
    ..complex from HeLa cells expressing CFP-SMN showed that the SMN protein interacts, as previously reported, with Gemin2 (SIP1), Gemin3 and Gemin4 and in addition associates with PPP4c...
  89. Peter C, Evans M, Thayanithy V, Taniguchi Ishigaki N, Bach I, Kolpak A, et al. The COPI vesicle complex binds and moves with survival motor neuron within axons. Hum Mol Genet. 2011;20:1701-11 pubmed publisher
    ..We propose that neurons utilize the Golgi-associated COPI vesicle to deliver cargoes necessary for motor neuron integrity and function. ..
  90. Gupta K, Martin R, Sharp R, Sarachan K, Ninan N, Van Duyne G. Oligomeric Properties of Survival Motor Neuron·Gemin2 Complexes. J Biol Chem. 2015;290:20185-99 pubmed publisher
    ..Here, we investigate the oligomeric nature of the SMN·Gemin2 complexes from humans and fission yeast (hSMN·Gemin2 and ySMN·Gemin2)...