galectin 4


Gene Symbol: galectin 4
Description: galectin 4
Alias: GAL4, L36LBP, galectin-4, L-36 lactose-binding protein, antigen NY-CO-27, gal-4, lactose-binding lectin 4, lectin, galactoside-binding, soluble, 4
Species: human
Products:     galectin 4

Top Publications

  1. Barondes S, Cooper D, Gitt M, Leffler H. Galectins. Structure and function of a large family of animal lectins. J Biol Chem. 1994;269:20807-10 pubmed
  2. Rechreche H, Mallo G, Montalto G, Dagorn J, Iovanna J. Cloning and expression of the mRNA of human galectin-4, an S-type lectin down-regulated in colorectal cancer. Eur J Biochem. 1997;248:225-30 pubmed
    ..These results suggest that decreased galectin-4 mRNA expression may be an early event in colon carcinogenesis. Among five cell lines derived from colon carcinoma, only two (HT29 and LS174T) expressed galectin-4 mRNA. ..
  3. Ideo H, Seko A, Ohkura T, Matta K, Yamashita K. High-affinity binding of recombinant human galectin-4 to SO(3)(-)-->3Galbeta1-->3GalNAc pyranoside. Glycobiology. 2002;12:199-208 pubmed
    ..These results suggest that galectin-4 has a unique carbohydrate binding specificity and interacts with O-linked sulfoglycans. ..
  4. Satelli A, Rao P, Thirumala S, Rao U. Galectin-4 functions as a tumor suppressor of human colorectal cancer. Int J Cancer. 2011;129:799-809 pubmed publisher
    ..Thus, gal-4 may prove to be an important molecule in understanding the biology of CRC. ..
  5. Long M, Campbell M. Integrative genomic approaches to dissect clinically-significant relationships between the VDR cistrome and gene expression in primary colon cancer. J Steroid Biochem Mol Biol. 2017;173:130-138 pubmed publisher
    ..g. LGALS4) whilst either stabilizing or not down-regulating expression of oncogenes (e.g. Carbonic Anhydrase 9 (CA9)). These integrative genomic approaches are relatively generic and applicable to the study of any transcription factor. ..
  6. Toribio M, Fitch K, Stone L, Zanni M, Lo J, de Filippi C, et al. Assessing statin effects on cardiovascular pathways in HIV using a novel proteomics approach: Analysis of data from INTREPID, a randomized controlled trial. EBioMedicine. 2018;35:58-66 pubmed publisher
    ..from KOWA Pharmaceuticals America, Inc. and the National Institutes of Health [P30 DK040561; Nutrition Obesity Research Center at Harvard]. M.T. was support by National Institutes of Health [5KL2TR001100-05; Harvard Catalyst KL2 grant]. ..
  7. Zhang D, Zhou C, Chen S, Yang Y, Shi B. Identification of hub genes and pathways associated with bladder cancer based on co-expression network analysis. Oncol Lett. 2017;14:1115-1122 pubmed publisher
  8. Đermadi D, Valo S, Pussila M, Reyhani N, Sarantaus L, Lalowski M, et al. Inherited cancer predisposition sensitizes colonic mucosa to address Western diet effects and putative cancer-predisposing changes on mouse proteome. J Nutr Biochem. 2014;25:1196-1206 pubmed publisher
    ..The significance of WD on CRC risk is highlighted by the fact that five out of six mice with neoplasias were fed with WD. ..
  9. El Leithy A, Helwa R, Assem M, Hassan N. Expression profiling of cancer-related galectins in acute myeloid leukemia. Tumour Biol. 2015;36:7929-39 pubmed publisher
    ..Hence, the higher expression of LGALS4 gene is associated with younger age (adjusted P value <0.001). In conclusion, galectin-12 may be a potential prognostic marker for AML. ..

More Information

Publications136 found, 100 shown here

  1. Schroder J, Zethner Moller R, Bové K, Mygind N, Hasbak P, Michelsen M, et al. Protein biomarkers and coronary microvascular dilatation assessed by rubidium-82 PET in women with angina pectoris and no obstructive coronary artery disease. Atherosclerosis. 2018;275:319-327 pubmed publisher
    ..5). Four biomarkers were significantly correlated with MBFR: Galectin-4 (Gal4, p?=?0.008), growth differentiation factor 15 (GDF15, p?=?0.026), tissue-type plasminogen activator (tPA, p?=?0...
  2. Molvin J, Pareek M, Jujic A, Melander O, Rastam L, Lindblad U, et al. Using a Targeted Proteomics Chip to Explore Pathophysiological Pathways for Incident Diabetes- The Malmö Preventive Project. Sci Rep. 2019;9:272 pubmed publisher
  3. Rodia M, Ugolini G, Mattei G, Montroni I, Zattoni D, Ghignone F, et al. Systematic large-scale meta-analysis identifies a panel of two mRNAs as blood biomarkers for colorectal cancer detection. Oncotarget. 2016;7:30295-306 pubmed publisher
    ..2%.Our preliminary study on a total of 134 subjects showed promising results for a blood screening test to be validated in a larger cohort with the staging stratification and in patients with other gastrointestinal diseases. ..
  4. Zhou J, Belov L, Chapuis P, Chan C, Armstrong N, Kaufman K, et al. Surface profiles of live colorectal cancer cells and tumor infiltrating lymphocytes from surgical samples correspond to prognostic categories. J Immunol Methods. 2015;416:59-68 pubmed publisher
    ..The groups from CRC and TIL profiles for systemic relapse showed 79.2% concordance, enabling prediction of relapse after surgery. The results demonstrate communication between CRC cells and TIL. ..
  5. El Ashram S, Al Nasr I, El Kemary M, Mehmood R, Hu M, Suo X. Early and late gene expression profiles of the ovine mucosa in response to Haemonchus contortus infection employing Illumina RNA-seq technology. Parasitol Int. 2017;66:681-692 pubmed publisher
    ..contortus infection, while galectin-4 is specifically down-regulated in the early infection. Additionally, our results showed that the induction or repression of these molecules is likely to determine the infection progression. ..
  6. Michaelsen S, Urup T, Olsen L, Broholm H, Lassen U, Poulsen H. Molecular profiling of short-term and long-term surviving patients identifies CD34 mRNA level as prognostic for glioblastoma survival. J Neurooncol. 2018;137:533-542 pubmed publisher
    ..Collectively, CD34 expression candidates as a prognostic biomarker in GBM able to identify survival outliers and could also be predictive for efficacy of bevacizumab. ..
  7. Holst S, Belo A, Giovannetti E, van Die I, Wuhrer M. Profiling of different pancreatic cancer cells used as models for metastatic behaviour shows large variation in their N-glycosylation. Sci Rep. 2017;7:16623 pubmed publisher
  8. Garcia C, Gómez Cañas M, Burgaz S, Palomares B, Gómez Gálvez Y, Palomo Garo C, et al. Benefits of VCE-003.2, a cannabigerol quinone derivative, against inflammation-driven neuronal deterioration in experimental Parkinson's disease: possible involvement of different binding sites at the PPARγ receptor. J Neuroinflammation. 2018;15:19 pubmed publisher
    ..2 in a Gal4-Luc system. However, T0070907 inhibited the effects of RGZ and VCE-003...
  9. Mondal T, Bag I, Sncvl P, Garikapati K, Bhadra U, Pal Bhadra M. Two way controls of apoptotic regulators consign DmArgonaute-1 a better clasp on it. PLoS ONE. 2018;13:e0190548 pubmed publisher
    ..Here we have over expressed Ago-1 in Drosophila eye and brain by employing UAS (upstream activation sequence)-GAL4 system under the expression of eye and brain specific driver...
  10. Wang P, Li J, Gong P, Wang W, Ai Y, Zhang X. An OTU deubiquitinating enzyme in Eimeria tenella interacts with Eimeria tenella virus RDRP. Parasit Vectors. 2018;11:74 pubmed publisher
    ..tenella OTU protein. A ?-galactosidase assay showed that the bait vector did not activate the GAL4 reporter gene, indicating no autoactivation activity from the RDRP bait fusion...
  11. Marikar F, Jin G, Sheng W, Ma D, Hua Z. Metallothionein 2A an interactive protein linking phosphorylated FADD to NF-?B pathway leads to colorectal cancer formation. Chin Clin Oncol. 2016;5:76 pubmed publisher
    ..biological role of the phosphorylated Fas associated death domain (pFADD) gene in colorectal cancer, we performed a GAL4-based yeast two-hybrid screening of a human heart cDNA library...
  12. Velentzas A, Anagnostopoulos A, Velentzas P, Mpakou V, Sagioglou N, Tsioka M, et al. Global Proteomic Profiling of Drosophila Ovary: A High-resolution, Unbiased, Accurate and Multifaceted Analysis. Cancer Genomics Proteomics. 2015;12:369-84 pubmed
    ..heterozygote fly strain that carries specific functions in the follicle cell compartment, and a reliable control in GAL4/UAS-based transgenic technology, we herein characterized the protein-expression profiling of D...
  13. Qi L, Xiang Z. Molecular cloning and expression analysis of an apoptosis-associated gene Daxx from zebrafish, Danio rerio. Fish Shellfish Immunol. 2015;45:59-66 pubmed publisher
    ..In the luciferase report assays, the GAL4-Daxx fusion protein inhibited the transcriptional activity of L8G5-Luc reporter gene showed that Daxx might act as ..
  14. Mysore K, Li P, Duman Scheel M. Identification of Aedes aegypti cis-regulatory elements that promote gene expression in olfactory receptor neurons of distantly related dipteran insects. Parasit Vectors. 2018;11:406 pubmed publisher
    ..Four CREs identified in the screen were cloned upstream of GAL4 in a transgenic construct that is compatible with transformation of a variety of insect species...
  15. Imura E, Yoshinari Y, Shimada Niwa Y, Niwa R. Protocols for Visualizing Steroidogenic Organs and Their Interactive Organs with Immunostaining in the Fruit Fly Drosophila melanogaster. J Vis Exp. 2017;: pubmed publisher
    ..the innervations of the ecdysteroidogenic organs can also be labeled by specific antibodies or a collection of GAL4 drivers in various types of neurons...
  16. Oh G, Yoon J, Lee G, Oh W, Kim S. 20(S)-protopanaxatriol inhibits liver X receptor α-mediated expression of lipogenic genes in hepatocytes. J Pharmacol Sci. 2015;128:71-7 pubmed publisher
    ..identified as an inhibitor against the ligand-dependent transactivation of liver X receptor α (LXRα) using a Gal4-TK-luciferase reporter system...
  17. Gyoergy A, Roblek M, Ratheesh A, Valoskova K, Belyaeva V, Wachner S, et al. Tools Allowing Independent Visualization and Genetic Manipulation of Drosophila melanogaster Macrophages and Surrounding Tissues. G3 (Bethesda). 2018;8:845-857 pubmed publisher
    ..These include extremely brightly fluorescing mCherry-based lines that allow GAL4-independent visualization of plasmatocyte nuclei, the cytoplasm, or the actin cytoskeleton from embryonic stage 8 ..
  18. Lee S, Bang S, Hong Y, Lee J, Jeong H, Park S, et al. The calcineurin inhibitor Sarah (Nebula) exacerbates Aβ42 phenotypes in a Drosophila model of Alzheimer's disease. Dis Model Mech. 2016;9:295-306 pubmed publisher
    ..Interestingly, overexpression of sra using the UAS-GAL4 system exacerbated the rough-eye phenotype, decreased survival rates and increased neuronal cell death in Aβ42-..
  19. Jackson H, Ono Y, Wang X, Elworthy S, Cunliffe V, Ingham P. The role of Sox6 in zebrafish muscle fiber type specification. Skelet Muscle. 2015;5:2 pubmed publisher
    ..The GAL4 binary misexpression system was used to express Sox6 ectopically in zebrafish embryos...
  20. Syrjänen L, Valanne S, Kuuslahti M, Tuomela T, Sriram A, Sanz A, et al. β carbonic anhydrase is required for female fertility in Drosophila melanogaster. Front Zool. 2015;12:19 pubmed publisher, we investigated the function of Drosophila β-CA by silencing the expression of the β-CA gene using UAS/GAL4-based RNA interference (RNAi) in Drosophila in vivo...
  21. Kroeger P, Shoue D, Mezzacappa F, Gerlach G, Wingert R, Schulz R. Knockdown of SCF(Skp2) function causes double-parked accumulation in the nucleus and DNA re-replication in Drosophila plasmatocytes. PLoS ONE. 2013;8:e79019 pubmed publisher
    ..eater-Gal4;UAS-GFP flies were crossed to UAS-RNAi lines to knockdown the function of all known SCF complex members in a ..
  22. Haussmann I, Ustaoğlu P, Bräuer U, Hemani Y, Dix T, Soller M. Plasmid-based gap-repair recombineered transgenes reveal a central role for introns in mutually exclusive alternative splicing in Down Syndrome Cell Adhesion Molecule exon 4. Nucleic Acids Res. 2018;: pubmed publisher
    ..UAS reporter genes for the Dscam variable exon 4 cluster and show that its alternative splicing is recapitulated by GAL4-mediated expression in neurons...
  23. M Angale P, Staveley B. Inhibition of mitochondrial calcium uptake 1 in Drosophila neurons. Genet Mol Res. 2017;16: pubmed publisher
    ..The inducible RNA interference-mediated inhibition of CG4495/MICU1 in the Ddc-Gal4-expressing neurons of Drosophila presented with reduction in survival coupled with a precocious loss of locomotor ..
  24. Roman E, Coman I, Prieto D, Alonso Monge R, Pla J. Implementation of a CRISPR-Based System for Gene Regulation in Candida albicans. mSphere. 2019;4: pubmed publisher
    ..i>Streptococcus pyogenes Cas9 devoid of nuclease activity to a transcriptional repressor (Nrg1) or activator (Gal4), we were able to show specific repression or activation of the tester gene CAT1, encoding the cytosolic ..
  25. Hand J, Zhang K, Wang L, Koganti P, Mastrantoni K, Rajput S, et al. Discovery of a novel oocyte-specific Krüppel-associated box domain-containing zinc finger protein required for early embryogenesis in cattle. Mech Dev. 2017;144:103-112 pubmed publisher
    ..KAP1), was demonstrated, and evidence supporting transcriptional repression by ZNFO was demonstrated using a GAL4-luciferase reporter system...
  26. Chakraborty M, Selma Soriano E, Magny E, Couso J, Pérez Alonso M, Charlet Berguerand N, et al. Pentamidine rescues contractility and rhythmicity in a Drosophila model of myotonic dystrophy heart dysfunction. Dis Model Mech. 2015;8:1569-78 pubmed publisher
    ..a Drosophila model expressing pure expanded CUG repeats under the control of the cardiomyocyte-specific driver GMH5-Gal4. Morphologically, expression of 250 CUG repeats caused abnormalities in the parallel alignment of the spiral ..
  27. Jones T, Parilla M, Jones P. Transgenic Drosophila for Investigating DUX4 and FRG1, Two Genes Associated with Facioscapulohumeral Muscular Dystrophy (FSHD). PLoS ONE. 2016;11:e0150938 pubmed publisher DUX4-fl and FRG1, we generated transgenic lines of Drosophila expressing either gene under control of the UAS/GAL4 binary system...
  28. Shir Shapira H, Sharabany J, Filderman M, Ideses D, Ovadia Shochat A, Mannervik M, et al. Structure-Function Analysis of the Drosophila melanogaster Caudal Transcription Factor Provides Insights into Core Promoter-preferential Activation. J Biol Chem. 2015;290:17293-305 pubmed publisher
    ..and the C terminus of Caudal was sufficient to confer core promoter-preferential activation to the heterologous GAL4 DNA-binding domain...
  29. Jermusyk A, Murphy N, Reeves G. Analyzing negative feedback using a synthetic gene network expressed in the Drosophila melanogaster embryo. BMC Syst Biol. 2016;10:85 pubmed publisher
    ..was accomplished using a synthetic network expressed in the Drosophila melanogaster embryo using the yeast proteins Gal4 (a transcriptional activator) and Gal80 (an inhibitor of Gal4 activity)...
  30. Hoshijima M, Hattori T, Aoyama E, Nishida T, Yamashiro T, Takigawa M. Roles of heterotypic CCN2/CTGF-CCN3/NOV and homotypic CCN2-CCN2 interactions in expression of the differentiated phenotype of chondrocytes. FEBS J. 2012;279:3584-3597 pubmed publisher
    To identify proteins that regulate CCN2 activity, we carried out GAL4-based yeast two-hybrid screening with a cDNA library derived from a chondrocytic cell line, HCS-2/8...
  31. Wang L, Dong J, Wei M, Wen W, Gao J, Zhang Z, et al. Selective and augmented β-glucuronidase expression combined with DOX-GA3 application elicits the potent suppression of prostate cancer. Oncol Rep. 2016;35:1417-24 pubmed publisher
    ..Intravenous injection of a GAL4 regulated bicistronic adenovirus vector constructed to express βG under the control of the PSA promoter (Ad/PSAP..
  32. M Angale P, Staveley B. Inhibition of Atg6 and Pi3K59F autophagy genes in neurons decreases lifespan and locomotor ability in Drosophila melanogaster. Genet Mol Res. 2016;15: pubmed publisher
    ..We first used the GMR-Gal4 driver to determine the effect of reducing the expression of the genes encoding these proteins on the developing ..
  33. Riveron J, Ibrahim S, Mulamba C, Djouaka R, Irving H, Wondji M, et al. Genome-Wide Transcription and Functional Analyses Reveal Heterogeneous Molecular Mechanisms Driving Pyrethroids Resistance in the Major Malaria Vector Anopheles funestus Across Africa. G3 (Bethesda). 2017;7:1819-1832 pubmed publisher
    ..The consistent overexpression of GSTe2 in Benin is coupled with a role of allelic variation at this gene as GAL4-UAS transgenic expression in Drosophila flies showed that the resistant 119F allele is highly efficient in ..
  34. Newman C, Toxopeus J, Udaka H, Ahn S, Martynowicz D, Graether S, et al. CRISPR-induced null alleles show that Frost protects Drosophila melanogaster reproduction after cold exposure. J Exp Biol. 2017;220:3344-3354 pubmed publisher an effective technique for completely knocking down genes, and is less likely to produce off-target effects than GAL4-UAS RNAi systems...
  35. Dasari V, Srivastava S, Khan S, Mishra R. Epigenetic factors Polycomb (Pc) and Suppressor of zeste (Su(z)2) negatively regulate longevity in Drosophila melanogaster. Biogerontology. 2018;19:33-45 pubmed publisher
    ..Using an inducible UAS-GAL4 system, we show that this effect is tissue-specific; knockdown in fat body, but not in muscle or brain tissues, ..
  36. Stampfel G, Kazmar T, Frank O, Wienerroither S, Reiter F, Stark A. Transcriptional regulators form diverse groups with context-dependent regulatory functions. Nature. 2015;528:147-51 pubmed publisher
    ..We recruited GAL4-DNA-binding-domain fusions of 812 Drosophila TFs and cofactors to 24 enhancer contexts and measured enhancer ..
  37. Chen X, Li T, Wang X, Du Z, Liu R, Yang Y. Synthetic dual-input mammalian genetic circuits enable tunable and stringent transcription control by chemical and light. Nucleic Acids Res. 2016;44:2677-90 pubmed publisher
    ..inducer, we created various chimeric promoters through the assembly of different copies of the tet operator and Gal4 operator module, which simultaneously responded to a tetracycline-responsive transcription factor and a light-..
  38. Otero M, Peng H, Hachem K, Culley K, Wondimu E, Quinn J, et al. ELF3 modulates type II collagen gene (COL2A1) transcription in chondrocytes by inhibiting SOX9-CBP/p300-driven histone acetyltransferase activity. Connect Tissue Res. 2017;58:15-26 pubmed publisher
    ..Co-transfection of ELF3 with a Gal4 reporter construct also inhibited Gal4-Sox9-driven transactivation, suggesting that ELF3 directly interacts with ..
  39. Piskacek M, Havelka M, Rezacova M, Knight A. The 9aaTAD Transactivation Domains: From Gal4 to p53. PLoS ONE. 2016;11:e0162842 pubmed publisher
    ..We found unforeseen similarity of the mammalian p53 with yeast Gal4 9aaTAD domains. Furthermore, we identified artificial 9aaTAD domains generated accidentally by others...
  40. Santos J, Vömel M, Struck R, Homberg U, Nässel D, Wegener C. Neuroarchitecture of peptidergic systems in the larval ventral ganglion of Drosophila melanogaster. PLoS ONE. 2007;2:e695 pubmed
    ..These connections can subsequently be more rigorously tested. By immunolabeling and GAL4-directed expression of marker proteins, we analyzed the projections and compartmentalization of neurons expressing ..
  41. Cai Z, Zeng Y, Xu B, Gao Y, Wang S, Zeng J, et al. Galectin-4 serves as a prognostic biomarker for the early recurrence / metastasis of hepatocellular carcinoma. Cancer Sci. 2014;105:1510-7 pubmed publisher
    ..Taken together, galectin-4 expression closely associates with HCC progression and might have potential use as a prognostic biomarker for HCC patients. ..
  42. Smith B, Ghazanfari A, Bohm R, Welch W, Zhang B, Masly J. A Flippase-Mediated GAL80/GAL4 Intersectional Resource for Dissecting Appendage Development in Drosophila. G3 (Bethesda). 2015;5:2105-12 pubmed publisher
    ..A recently developed Flippase-induced intersectional GAL80/GAL4 repression method incorporates several gene manipulation technologies in Drosophila to enable such fine-scale ..
  43. Almeida R, Lyons D. Intersectional Gene Expression in Zebrafish Using the Split KalTA4 System. Zebrafish. 2015;12:377-86 pubmed publisher
    In this study, we describe the adaptation of the split Gal4 system for zebrafish...
  44. Lacin H, Truman J. Lineage mapping identifies molecular and architectural similarities between the larval and adult Drosophila central nervous system. elife. 2016;5:e13399 pubmed publisher
    ..the embryonic neuroblast origin of the adult neuronal lineages in the ventral nervous system via lineage-specific GAL4 lines and molecular markers...
  45. Förster D, Dal Maschio M, Laurell E, Baier H. An optogenetic toolbox for unbiased discovery of functionally connected cells in neural circuits. Nat Commun. 2017;8:116 pubmed publisher
    ..By combining the Gal4-UAS system with Cre/lox recombination, we target the optogenetic actuator ChrimsonR and the sensor GCaMP6 to ..
  46. Linneweber G, Winking M, Fischbach K. The Cell Adhesion Molecules Roughest, Hibris, Kin of Irre and Sticks and Stones Are Required for Long Range Spacing of the Drosophila Wing Disc Sensory Sensilla. PLoS ONE. 2015;10:e0128490 pubmed publisher
    ..Using mutant analysis the GAL4/UAS system, RNAi and gain of function we found an involvement of all four Irre Cell Recognition Module-proteins in ..
  47. Dutta D, Dobson A, Houtz P, Gläßer C, Revah J, Korzelius J, et al. Regional Cell-Specific Transcriptome Mapping Reveals Regulatory Complexity in the Adult Drosophila Midgut. Cell Rep. 2015;12:346-58 pubmed publisher
    ..We validate cell-specific and regional Gal4 drivers; demonstrate roles for transporter Smvt and transcription factors GATAe, Sna, and Ptx1 in global and ..
  48. Helwa R, Ramadan M, Abdel Wahab A, Knappskog S, Bauer A. Promoter SNPs rs116896264 and rs73933062 form a distinct haplotype and are associated with galectin-4 overexpression in colorectal cancer. Mutagenesis. 2016;31:401-8 pubmed publisher
    ..Both in the patient samples and in the 1000 genomes project, the two SNPs were found to co-occur whenever present (D' = 1). ..
  49. M Angale P, Staveley B. A loss of Pdxk model of Parkinson disease in Drosophila can be suppressed by Buffy. BMC Res Notes. 2017;10:205 pubmed publisher
    ..possesses a single homologue of Pdxk and we used RNA interference to inhibit the activity of this kinase in the Ddc-Gal4-expressing neurons...
  50. Alvarez M, Ruiz M, Sánchez L. Effect of the gene doublesex of anastrepha on the somatic sexual development of Drosophila. PLoS ONE. 2009;4:e5141 pubmed publisher
    ..To express these proteins in Drosophila, the GAL4-UAS system was used...
  51. Shaposhnikov M, Proshkina E, Shilova L, Zhavoronkov A, Moskalev A. Lifespan and Stress Resistance in Drosophila with Overexpressed DNA Repair Genes. Sci Rep. 2015;5:15299 pubmed publisher
    ..In this study, we investigated the effects of GAL4-induced overexpression of genes implicated in DNA repair on lifespan and resistance to stress factors in Drosophila ..
  52. Sun M, Zeng X, Xie W. Temporal and spatial expression of Drosophila Neurexin during the life cycle visualized using a DNRX-Gal4/UAS-reporter. Sci China Life Sci. 2016;59:68-77 pubmed publisher
    ..For this study, we generated a novel Drosophila transgenic strain termed the DNRX-Gal4 transgenic line, with characteristic features in agreement with the endogenous DNRX expression pattern...
  53. Park S, Littleton J, Park H, Lee J. Drosophila Homolog of Human KIF22 at the Autism-Linked 16p11.2 Loci Influences Synaptic Connectivity at Larval Neuromuscular Junctions. Exp Neurobiol. 2016;25:33-9 pubmed publisher
    ..We conducted a preliminary genetic screen based on RNA interference in combination with the GAL4-UAS system, followed by mutational analyses...
  54. Clark M, McCumsey S, Lopez Darwin S, Heckscher E, Doe C. Functional Genetic Screen to Identify Interneurons Governing Behaviorally Distinct Aspects of Drosophila Larval Motor Programs. G3 (Bethesda). 2016;6:2023-31 pubmed publisher
    ..We used 165 Janelia CRM-Gal4 lines-chosen for sparse neuronal expression-to ectopically express the warmth-inducible neuronal activator TrpA1, ..
  55. Katsu Y, Kohno S, Oka K, Baker M. Evolution of corticosteroid specificity for human, chicken, alligator and frog glucocorticoid receptors. Steroids. 2016;113:38-45 pubmed publisher
    ..activation of truncated GRs containing their hinge domain and ligand binding domain (LBD) fused to a GAL4 DNA binding domain (GAL4-DBD)...
  56. Lin H. Paeoniflorin acts as a liver X receptor agonist. J Asian Nat Prod Res. 2013;15:35-45 pubmed publisher
    ..The results showed that paeoniflorin transactivated GAL4, rat cholesterol 7 ?-hydroxylase, phospholipid transfer protein, and ATP-binding cassette A1 gene promoters in dose-..
  57. Singh A, Lakhotia S. Expression of hsrω-RNAi transgene prior to heat shock specifically compromises accumulation of heat shock-induced Hsp70 in Drosophila melanogaster. Cell Stress Chaperones. 2016;21:105-120 pubmed publisher and stress-inducible noncoding hsrω-n transcripts were down-regulated during heat shock through hs-GAL4-driven expression of the hsrω-RNAi transgene, despite the characteristic elevation of all heat shock proteins (Hsp)..
  58. Sellami A, Veenstra J. SIFamide acts on fruitless neurons to modulate sexual behavior in Drosophila melanogaster. Peptides. 2015;74:50-6 pubmed publisher
    ..If neurons expressing a SIFaR-gal4 transgene are killed by the apoptotic protein reaper or when these neurons express SIFamide receptor RNAi, males ..
  59. M Angale P, Staveley B. The Bcl-2 homologue Buffy rescues ?-synuclein-induced Parkinson disease-like phenotypes in Drosophila. BMC Neurosci. 2016;17:24 pubmed publisher
    ..To alter the expression of Buffy in the dopaminergic neurons of Drosophila, the Ddc-Gal4 transgene was used...
  60. Piskacek M, Havelka M, Rezacova M, Knight A. The 9aaTAD Is Exclusive Activation Domain in Gal4. PLoS ONE. 2017;12:e0169261 pubmed publisher
    The Gal4 protein is a well-known prototypic acidic activator that has multiple activation domains. We have previously identified a new activation domain called the nine amino acid transactivation domain (9aaTAD) in Gal4 protein...
  61. Juárez Morales J, MARTINEZ DE LUNA R, Zuber M, Roberts A, Lewis K. Zebrafish transgenic constructs label specific neurons in Xenopus laevis spinal cord and identify frog V0v spinal neurons. Dev Neurobiol. 2017;77:1007-1020 pubmed publisher
    ..laevis and that the incorporation of a Gal4:UAS amplification cassette enables cells to be observed in live X. laevis tadpoles...
  62. Mizoguchi T, Kawakami K, Itoh M. Zebrafish lines expressing UAS-driven red probes for monitoring cytoskeletal dynamics. Genesis. 2016;54:483-9 pubmed publisher
    ..Lifeact or EB1 that interact with actin filaments and microtubule plus ends, respectively, under the control of the GAL4-UAS system...
  63. Caygill E, Brand A. The GAL4 System: A Versatile System for the Manipulation and Analysis of Gene Expression. Methods Mol Biol. 2016;1478:33-52 pubmed
    Since its introduction in 1993, the GAL4 system has become an essential part of the Drosophila geneticist's toolkit...
  64. Jeibmann A, Schulz J, Eikmeier K, Johann P, Thiel K, Tegeder I, et al. SMAD dependent signaling plays a detrimental role in a fly model of SMARCB1-deficiency and the biology of atypical teratoid/rhabdoid tumors. J Neurooncol. 2017;131:477-484 pubmed publisher
    ..Using Drosophila melanogaster and the Gal4-UAS system, modifier screens were performed in order to identify the role of SMAD dependent signaling in the lethal ..
  65. Tian H, Wang X, Guo H, Cheng Y, Hou C, Chen J, et al. NTL8 Regulates Trichome Formation in Arabidopsis by Directly Activating R3 MYB Genes TRY and TCL1. Plant Physiol. 2017;174:2363-2375 pubmed publisher
    ..When recruited to the promoter region of the reporter gene Gal4:GUS by a fused GAL4 DNA-binding domain, NTL8 activated the expression of the reporter gene...
  66. Dal Pra M, Carta D, Szabadkai G, Suman M, Frión Herrera Y, Paccagnella N, et al. Targeting RORs nuclear receptors by novel synthetic steroidal inverse agonists for autoimmune disorders. Bioorg Med Chem. 2018;26:1686-1704 pubmed publisher
    ..The selected 7, 8, 19a and 31 compounds were assayed in a Gal4 UAS-Luc co-transfection system in order to determine their ability to modulate ROR?t activity in a cellular ..
  67. Li Z, Cheng D, Mon H, Tatsuke T, Zhu L, Xu J, et al. Genome-wide identification of polycomb target genes reveals a functional association of Pho with Scm in Bombyx mori. PLoS ONE. 2012;7:e34330 pubmed publisher its inhibitory function and ectopic overexpression of BmScm is able to promote transcriptional repression by Gal4-Pho fusions including BmScm-interacting domain...
  68. Yan X, Liao H, Cheng M, Shi X, Lin X, Feng X, et al. Smad7 Protein Interacts with Receptor-regulated Smads (R-Smads) to Inhibit Transforming Growth Factor-β (TGF-β)/Smad Signaling. J Biol Chem. 2016;291:382-92 pubmed publisher
    ..oligomerized with R-Smad proteins upon TGF-β signaling and directly inhibited R-Smad activity, as assessed by Gal4-luciferase reporter assays...
  69. Nakajima M, Watanabe T, Aoki R, Shimizu R, Okuyama S, Furukawa Y. Phenotypes associated with psychiatric disorders are sex-specific in a mutant mouse line. Brain Res. 2016;1652:53-61 pubmed publisher
    Wnt1-Cre- and Wnt1-GAL4 double transgenic (dTg) mice are used to study neural crest cell lineages by utilizing either the Cre/loxP or the GAL4/UAS system...
  70. Arasu A, Kumaresan V, Ganesh M, Pasupuleti M, Arasu M, Al Dhabi N, et al. Bactericidal activity of fish galectin 4 derived membrane-binding peptide tagged with oligotryptophan. Dev Comp Immunol. 2017;71:37-48 pubmed publisher
    ..Our results suggested that addition of W5 at the C-terminal of membrane-binding peptide remarkably improved its membrane disrupting activity. ..
  71. Negeri D, Eggert H, Gienapp R, Saumweber H. Inducible RNA interference uncovers the Drosophila protein Bx42 as an essential nuclear cofactor involved in Notch signal transduction. Mech Dev. 2002;117:151-62 pubmed
    We used the UAS/GAL4 two component system to induce mRNA interference (mRNAi) during Drosophila development...
  72. Stowell S, Karmakar S, Stowell C, Dias Baruffi M, McEver R, Cummings R. Human galectin-1, -2, and -4 induce surface exposure of phosphatidylserine in activated human neutrophils but not in activated T cells. Blood. 2007;109:219-27 pubmed
    ..These results suggest novel immunoregulatory contribution of galectins in regulating leukocyte turnover independently of apoptosis. ..
  73. Bum Erdene K, Leffler H, Nilsson U, Blanchard H. Structural characterization of human galectin-4 C-terminal domain: elucidating the molecular basis for recognition of glycosphingolipids, sulfated saccharides and blood group antigens. FEBS J. 2015;282:3348-67 pubmed publisher
    ..4ylz, 4ym0, 4ym1, 4ym2, 4ym3. ..
  74. Wang Y, Li Y, Hu G, Huang X, Rao H, Xiong X, et al. Nek2A phosphorylates and stabilizes SuFu: A new strategy of Gli2/Hedgehog signaling regulatory mechanism. Cell Signal. 2016;28:1304-13 pubmed publisher
    ..Here, we performed a GAL4-based yeast two-hybrid screen using human SuFu as bait, and identified NIMA-related expressed kinase 2A (Nek2A) as ..
  75. Nio Kobayashi J. Tissue- and cell-specific localization of galectins, ?-galactose-binding animal lectins, and their potential functions in health and disease. Anat Sci Int. 2017;92:25-36 pubmed
    ..The ligand glycoconjugate structures mediated by glycosyltransferases including MGAT5, ST6GAL1, and C2GnT are important for revealing the functions of galectins in healthy and disease states. ..
  76. Yonemura Y, Futai E, Yagishita S, Kaether C, Ishiura S. Specific combinations of presenilins and Aph1s affect the substrate specificity and activity of ?-secretase. Biochem Biophys Res Commun. 2016;478:1751-7 pubmed publisher
    ..Intriguingly, PS2/Aph1b had a clear substrate specificity: APP-Gal4, but not Notch-Gal4, was cleaved...
  77. Kyrchanova O, Chetverina D, Maksimenko O, Kullyev A, Georgiev P. Orientation-dependent interaction between Drosophila insulators is a property of this class of regulatory elements. Nucleic Acids Res. 2008;36:7019-28 pubmed publisher
    ..such as scs-scs, scs'-scs', 1A2-1A2 and Wari-Wari support distant activation of the white promoter by the yeast GAL4 activator in an orientation-dependent manner...
  78. Duray A, De Maesschalck T, Decaestecker C, Remmelink M, Chantrain G, Neiveyans J, et al. Galectin fingerprinting in naso-sinusal diseases. Oncol Rep. 2014;32:23-32 pubmed publisher
    ..In addition, we observed that the expression of galectin in naso-sinusal diseases seems to be affected by tumor progression and not inflammatory or allergic phenomena. ..
  79. Wen D, Zheng L, Ni L, Wang H, Feng Y, Zhang M. The expression of CG9940 affects the adaptation of cardiac function, mobility, and lifespan to exercise in aging Drosophila. Exp Gerontol. 2016;83:6-14 pubmed publisher
    ..In our study, the expression of CG9940 was changed by UAS/GAL4 system in Drosophila. Flies were trained by a training device...
  80. Marcogliese P, Abuaish S, Kabbach G, Abdel Messih E, Seang S, Li G, et al. LRRK2(I2020T) functional genetic interactors that modify eye degeneration and dopaminergic cell loss in Drosophila. Hum Mol Genet. 2017;26:1247-1257 pubmed publisher
    ..However, the fruit fly has been exploited to recapitulate PD gene related dopaminergic cell loss. Using the GAL4-UAS system and established models of hLRRK2 induced eye degeneration in Drosophila, we conducted an unbiased ..
  81. Helwa R, Heller A, Knappskog S, Bauer A. Tumor cells interact with red blood cells via galectin-4 - a short report. Cell Oncol (Dordr). 2017;40:401-409 pubmed publisher
    ..Conversely, we found that RBC attachment induces galectin-4 expression in tumor cells. From our data we conclude that tumor cells directly interact with red blood cells via galectin-4. ..
  82. Ideo H, Seko A, Yamashita K. Recognition mechanism of galectin-4 for cholesterol 3-sulfate. J Biol Chem. 2007;282:21081-9 pubmed
    ..These results suggested that not only sulfated glycosphingolipids but also cholesterol 3-sulfate are endogenous ligands for galectin-4 in vivo. ..
  83. Oberg C, Blanchard H, Leffler H, Nilsson U. Protein subtype-targeting through ligand epimerization: talose-selectivity of galectin-4 and galectin-8. Bioorg Med Chem Lett. 2008;18:3691-4 pubmed publisher
    ..Galectin-4C and 8N were found to prefer the d-talopyranose configuration to the natural ligand d-galactopyranose configuration. Derivatization at talose O2 and/or O3 provided selective submillimolar inhibitors for these two galectins. ..
  84. Kim S, Park K, Jeon S, Ohn T, Kim T, Kim W, et al. Abrogation of galectin-4 expression promotes tumorigenesis in colorectal cancer. Cell Oncol (Dordr). 2013;36:169-78 pubmed publisher
  85. Niu H, Hadwiger G, Fujiwara H, Welch J. Pathways of retinoid synthesis in mouse macrophages and bone marrow cells. J Leukoc Biol. 2016;99:797-810 pubmed publisher
    ..With the use of a upstream activation sequence-GFP reporter transgene and retrovirally expressed Gal4-retinoic acid receptor α in primary mouse bone marrow cells, we identified 2 distinct enzymatic pathways used by ..
  86. Díez Revuelta N, Higuero A, Velasco S, Peñas de la Iglesia M, Gabius H, Abad Rodriguez J. Neurons define non-myelinated axon segments by the regulation of galectin-4-containing axon membrane domains. Sci Rep. 2017;7:12246 pubmed publisher
  87. Liu P, Sharrock R. Biological activity and dimerization state of modified phytochrome A proteins. PLoS ONE. 2017;12:e0186468 pubmed publisher
    ..Dimers of the phyA 1-615 amino acid N-terminal photosensory domain (NphyA), generated in vivo with a yeast GAL4 dimerization domain and attached to a constitutive nuclear localization sequence, are expressed at a low level and, ..
  88. Ørsted M, Rohde P, Hoffmann A, Sørensen P, Kristensen T. Environmental variation partitioned into separate heritable components. Evolution. 2017;: pubmed publisher
    ..Using the binary UAS-GAL4 system, we functionally validated the effects of a subset of candidate genes affecting each of the four components ..
  89. Jang Y, Chae H, Kim Y. Female-specific myoinhibitory peptide neurons regulate mating receptivity in Drosophila melanogaster. Nat Commun. 2017;8:1630 pubmed publisher
    ..Both vAL and vAM express myoinhibitory peptide (Mip)-GAL4. vAL is positive for Mip neuropeptides and the sex-determining transcriptional factor doublesex...
  90. Chen Y, Dahanukar A. Molecular and Cellular Organization of Taste Neurons in Adult Drosophila Pharynx. Cell Rep. 2017;21:2978-2991 pubmed publisher
    ..generate receptor-to-neuron maps of all three pharyngeal taste organs by performing a comprehensive chemoreceptor-GAL4/LexA expression analysis...
  91. Zimbardi A, Pinheiro M, Dias Baruffi M, Nonato M. Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of the N-terminal carbohydrate-recognition domain of human galectin-4. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010;66:542-5 pubmed publisher
    ..The crystals belonged to space group P6(1)22, with unit-cell parameters a = b = 71.25, c = 108.66 A. The asymmetric unit contained one molecule of hGal4-CRD-1, with a V(M) value of 2.34 A(3) Da(-1) and a solvent content of 47.51%. ..
  92. Eisenhoffer G, Slattum G, Ruiz O, Otsuna H, Bryan C, Lopez J, et al. A toolbox to study epidermal cell types in zebrafish. J Cell Sci. 2017;130:269-277 pubmed publisher to fluorescently tag specific epithelial cell types and express genes in a mosaic fashion using five Gal4 lines identified from an enhancer trap screen...