gag-pol

Summary

Gene Symbol: gag-pol
Description: Gag-Pol
Alias: Gag-Pol polyprotein
Species: Human immunodeficiency virus 1

Top Publications

  1. ncbi Characterization and structural analysis of HIV-1 integrase conservation
    Francesca Ceccherini-Silberstein
    Department of Experimental Medicine, University of Rome Tor Vergata, Rome, Italy
    AIDS Rev 11:17-29. 2009
  2. doi Transportin-SR2 imports HIV into the nucleus
    Frauke Christ
    Laboratory for Molecular Virology and Gene Therapy, KU Leuven and IRC, Kapucijnenvoer 33, B 3000 Leuven, Flanders, Belgium
    Curr Biol 18:1192-202. 2008
  3. pmc Cytidine deaminases APOBEC3G and APOBEC3F interact with human immunodeficiency virus type 1 integrase and inhibit proviral DNA formation
    Kun Luo
    Department of Microbiology and Immunology, Johns Hopkins Bloomberg School of Public Health, 615 N Wolfe Street, Baltimore, MD 21205, USA
    J Virol 81:7238-48. 2007
  4. pmc A genome-wide short hairpin RNA screening of jurkat T-cells for human proteins contributing to productive HIV-1 replication
    Man Lung Yeung
    Molecular Virology Section, Laboratory of Molecular Microbiology, NIAID, National Institutes of Health, Bethesda, Maryland 20892 0460, USA
    J Biol Chem 284:19463-73. 2009
  5. pmc Functional coupling between HIV-1 integrase and the SWI/SNF chromatin remodeling complex for efficient in vitro integration into stable nucleosomes
    Paul Lesbats
    Laboratoire MCMP, UMR 5234 CNRS Université Victor Segalen Bordeaux 2, Bordeaux, France
    PLoS Pathog 7:e1001280. 2011
  6. ncbi HIV-1 integrase forms stable tetramers and associates with LEDGF/p75 protein in human cells
    Peter Cherepanov
    Rega Institute for Medical Research, Katholieke Universiteit Leuven, Minderbroedersstraat 10, B 3000, Leuven, Belgium
    J Biol Chem 278:372-81. 2003
  7. doi Yeast two-hybrid detection of integrase-host factor interactions
    Jean Christophe Rain
    Hybrigenics S A, 3 5 Impasse Reille, Paris 75014, France
    Methods 47:291-7. 2009
  8. ncbi Identification of the LEDGF/p75 binding site in HIV-1 integrase
    Katrien Busschots
    Laboratory for Molecular Virology and Gene Therapy, Molecular Medicine, Katholieke Universiteit Leuven, Flanders, Belgium
    J Mol Biol 365:1480-92. 2007
  9. ncbi Binding and stimulation of HIV-1 integrase by a human homolog of yeast transcription factor SNF5
    G V Kalpana
    Howard Hughes Medical Institute, Department of Biochemistry and Molecular Biophysics, Columbia University, College of Physicians and Surgeons, New York, NY 10032
    Science 266:2002-6. 1994
  10. pmc Structure-function analysis of integrase interactor 1/hSNF5L1 reveals differential properties of two repeat motifs present in the highly conserved region
    A Morozov
    Department of Molecular Genetics, Albert Einstein College of Medicine, 1300 Morris Park Avenue, U821, Bronx, NY 10461, USA
    Proc Natl Acad Sci U S A 95:1120-5. 1998

Research Grants

  1. BIOCHEMICAL MECHANISM OF HIV DNA INTEGRATION
    Alan Engelman; Fiscal Year: 2004
  2. HIV-1 Preintegration Trafficking and Nuclear Localization
    Alan Engelman; Fiscal Year: 2007
  3. HIV-1 Integrase Structural Biology
    Alan Engelman; Fiscal Year: 2007
  4. MECHANISM OF NON-INTEGRASE-MEDIATED HIV-1 REPLICATION
    Alan Engelman; Fiscal Year: 2001
  5. Nuclear Localization of HIV-1 Preintegration Complexes
    Alan Engelman; Fiscal Year: 2006
  6. LEDGF-Integrase Structural Biology
    Alan Engelman; Fiscal Year: 2005
  7. Nuclear Localization of HIV-1 Preintegration Complexes
    Alan Engelman; Fiscal Year: 2003
  8. BIOCHEMICAL MECHANISM OF HIV DNA INTEGRATION
    Alan Engelman; Fiscal Year: 2009
  9. HIV-1 Preintegration Trafficking and Nuclear Localization
    ALAN N ENGELMAN; Fiscal Year: 2010
  10. HIV-1 Integrase Structural Biology
    ALAN N ENGELMAN; Fiscal Year: 2010

Detail Information

Publications145 found, 100 shown here

  1. ncbi Characterization and structural analysis of HIV-1 integrase conservation
    Francesca Ceccherini-Silberstein
    Department of Experimental Medicine, University of Rome Tor Vergata, Rome, Italy
    AIDS Rev 11:17-29. 2009
    ....
  2. doi Transportin-SR2 imports HIV into the nucleus
    Frauke Christ
    Laboratory for Molecular Virology and Gene Therapy, KU Leuven and IRC, Kapucijnenvoer 33, B 3000 Leuven, Flanders, Belgium
    Curr Biol 18:1192-202. 2008
    ..This requires import of the preintegration complex (PIC) through the nuclear pore. Although many cellular and viral determinants have been proposed, the mechanism leading to nuclear import is not yet understood...
  3. pmc Cytidine deaminases APOBEC3G and APOBEC3F interact with human immunodeficiency virus type 1 integrase and inhibit proviral DNA formation
    Kun Luo
    Department of Microbiology and Immunology, Johns Hopkins Bloomberg School of Public Health, 615 N Wolfe Street, Baltimore, MD 21205, USA
    J Virol 81:7238-48. 2007
    ..Thus, multiple steps of the HIV-1 replication cycle, most noticeably the formation of proviral DNA, are inhibited by both cytidine deamination-dependent and -independent mechanisms...
  4. pmc A genome-wide short hairpin RNA screening of jurkat T-cells for human proteins contributing to productive HIV-1 replication
    Man Lung Yeung
    Molecular Virology Section, Laboratory of Molecular Microbiology, NIAID, National Institutes of Health, Bethesda, Maryland 20892 0460, USA
    J Biol Chem 284:19463-73. 2009
    ..We discuss the similarities and differences between our shRNA findings for HIV-1 using a spreading infection assay in human Jurkat T-cells and results from other investigators who used siRNA-based screenings in HeLa or 293T cells...
  5. pmc Functional coupling between HIV-1 integrase and the SWI/SNF chromatin remodeling complex for efficient in vitro integration into stable nucleosomes
    Paul Lesbats
    Laboratoire MCMP, UMR 5234 CNRS Université Victor Segalen Bordeaux 2, Bordeaux, France
    PLoS Pathog 7:e1001280. 2011
    ..Our data indicate that some chromatin structures can be refractory for integration and that coupling between nucleosome remodeling and HIV-1 integration is required to overcome this natural barrier...
  6. ncbi HIV-1 integrase forms stable tetramers and associates with LEDGF/p75 protein in human cells
    Peter Cherepanov
    Rega Institute for Medical Research, Katholieke Universiteit Leuven, Minderbroedersstraat 10, B 3000, Leuven, Belgium
    J Biol Chem 278:372-81. 2003
    ....
  7. doi Yeast two-hybrid detection of integrase-host factor interactions
    Jean Christophe Rain
    Hybrigenics S A, 3 5 Impasse Reille, Paris 75014, France
    Methods 47:291-7. 2009
    ..All tolled, our studies identified LEDGF/p75, Transportin-SR2 (TNPO3), von Hippel-Lindau binding protein 1 (VBP1), and sucrose non-fermenting 5 (SNF5) as cellular binding partners of HIV-1 integrase...
  8. ncbi Identification of the LEDGF/p75 binding site in HIV-1 integrase
    Katrien Busschots
    Laboratory for Molecular Virology and Gene Therapy, Molecular Medicine, Katholieke Universiteit Leuven, Flanders, Belgium
    J Mol Biol 365:1480-92. 2007
    ..Comparison of the wild-type IN-LEDGF/p75 co-crystal structure with that of the modelled structure of the IN(Q168A) and IN(W131A) mutant integrases corroborated our experimental data...
  9. ncbi Binding and stimulation of HIV-1 integrase by a human homolog of yeast transcription factor SNF5
    G V Kalpana
    Howard Hughes Medical Institute, Department of Biochemistry and Molecular Biophysics, Columbia University, College of Physicians and Surgeons, New York, NY 10032
    Science 266:2002-6. 1994
    ..The gene, termed INI1 (for integrase interactor 1), may encode a nuclear factor that promotes integration and targets incoming viral DNA to active genes...
  10. pmc Structure-function analysis of integrase interactor 1/hSNF5L1 reveals differential properties of two repeat motifs present in the highly conserved region
    A Morozov
    Department of Molecular Genetics, Albert Einstein College of Medicine, 1300 Morris Park Avenue, U821, Bronx, NY 10461, USA
    Proc Natl Acad Sci U S A 95:1120-5. 1998
    ....
  11. ncbi Measuring protein-protein interactions inside living cells using single color fluorescence correlation spectroscopy. Application to human immunodeficiency virus type 1 integrase and LEDGF/p75
    Goedele Maertens
    Laboratory of Biomolecular Dynamics, Katholieke Universiteit Leuven, Leuven, Belgium
    FASEB J 19:1039-41. 2005
    ..We thus demonstrate that protein-protein interactions can be studied in living cells, using single-color FCM (scFCM)...
  12. pmc HIV-1 protease cleaves eukaryotic initiation factor 4G and inhibits cap-dependent translation
    I Ventoso
    Centro de Biologia Molecular Severo Ochoa, Universidad Autonoma de Madrid, Facultad de Ciencias, Cantoblanco, 28049 Madrid, Spain
    Proc Natl Acad Sci U S A 98:12966-71. 2001
    ..These findings indicate that HIV-1, a member of retrovirus group, shares with picornaviruses the capacity to proteolyze eIF4G...
  13. pmc Host protein Ku70 binds and protects HIV-1 integrase from proteasomal degradation and is required for HIV replication
    Yingfeng Zheng
    Laboratory of Molecular Human Retrovirology, Department of Medical Microbiology, Faculty of Medicine, University of Manitoba, Winnipeg, Manitoba R3E 0J9, Canada
    J Biol Chem 286:17722-35. 2011
    ..Overall, this report provides another example of how HIV-1 hijacks host cellular machinery to protect the virus itself and to facilitate its replication...
  14. pmc Human immunodeficiency virus type 1 preintegration complexes: studies of organization and composition
    M D Miller
    Infectious Disease Laboratory, The Salk Institute for Biological Studies, La Jolla, California 92037, USA
    J Virol 71:5382-90. 1997
    ..Only molecules correctly cleaved by integrase protein at the 3' ends were competent to integrate, suggesting that one role for terminal cleavage by integrase may be to create a defined end at otherwise heterogeneous cDNA termini...
  15. ncbi LEDGF/p75 is essential for nuclear and chromosomal targeting of HIV-1 integrase in human cells
    Goedele Maertens
    Laboratory of Biomolecular Dynamics, Katholieke Universiteit Leuven, Celestijnenlaan 200D, B 3001 Leuven, Belgium
    J Biol Chem 278:33528-39. 2003
    ..We conclude, therefore, that the interaction with LEDGF/p75 accounts for the karyophilic properties and chromosomal targeting of HIV-1 IN...
  16. pmc Deaminase-independent inhibition of HIV-1 reverse transcription by APOBEC3G
    Yasumasa Iwatani
    Laboratory of Molecular Genetics, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    Nucleic Acids Res 35:7096-108. 2007
    ..These data support a novel mechanism for deaminase-independent inhibition of reverse transcription that is determined by critical differences in the nucleic acid binding properties of A3G, NC and RT...
  17. pmc Amino-terminal polypeptides of vimentin are responsible for the changes in nuclear architecture associated with human immunodeficiency virus type 1 protease activity in tissue culture cells
    R L Shoeman
    Max Planck Institut für Zellbiologie, Rosenhof, D 68526 Ladenburg, Germany
    Mol Biol Cell 12:143-54. 2001
    ..The ability of HIV-1 to affect nuclear organization via the liberation of vimentin peptides may play an important role in HIV-1-associated cytopathogenesis and carcinogenesis...
  18. pmc Correlation of recombinant integrase activity and functional preintegration complex formation during acute infection by replication-defective integrase mutant human immunodeficiency virus
    Xiang Li
    Address correspondence to Alan Engelman
    J Virol 86:3861-79. 2012
    ....
  19. pmc von Hippel Lindau binding protein 1-mediated degradation of integrase affects HIV-1 gene expression at a postintegration step
    Aurelie Mousnier
    Institut Jacques Monod, Centre National de la Recherche Scientifique, Universités Paris 6 et 7, F 75251 Paris, France
    Proc Natl Acad Sci U S A 104:13615-20. 2007
    ..Results presented here support a role for integrase degradation by the prefoldin-VHL-proteasome pathway in the integration-transcription transition of the viral replication cycle...
  20. doi Inhibitors of the interactions between HIV-1 IN and the cofactor LEDGF/p75
    Laura De Luca
    Dipartimento Farmaco Chimico, Universita degli Studi di Messina, Viale Annunziata, 98168 Messina, Italy
    ChemMedChem 6:1184-91. 2011
    ..We describe herein the progress to date in developing inhibitors of this promising target...
  21. pmc The large subunit of HIV-1 reverse transcriptase interacts with beta-actin
    M Hottiger
    Department of Veterinary Biochemistry, University of Zurich Irchel, Switzerland
    Nucleic Acids Res 23:736-41. 1995
    ..The reverse transcriptase/beta-actin interaction might be important for the secretion of HIV-1 virions...
  22. pmc N348I in HIV-1 reverse transcriptase can counteract the nevirapine-mediated bias toward RNase H cleavage during plus-strand initiation
    Mia J Biondi
    Department of Microbiology and Immunology, McGill University, Montreal, Quebec H3A 2B4, Canada
    J Biol Chem 285:26966-75. 2010
    ..The data are in agreement with clinical data, which demonstrate a stronger effect of N348I on susceptibility to nevirapine as compared with efavirenz...
  23. pmc Multimerization and DNA binding properties of INI1/hSNF5 and its functional significance
    Supratik Das
    Department of Molecular Genetics, Albert Einstein College of Medicine, New York, New York 10461, USA
    J Biol Chem 284:19903-14. 2009
    ....
  24. ncbi An essential role for LEDGF/p75 in HIV integration
    Manuel Llano
    Molecular Medicine Program, Mayo Clinic College of Medicine, Rochester, MN 55905, USA
    Science 314:461-4. 2006
    ..Perturbing the p75-integrase interaction may have therapeutic potential...
  25. ncbi A role for LEDGF/p75 in targeting HIV DNA integration
    Angela Ciuffi
    University of Pennsylvania School of Medicine, Department of Microbiology, 3610 Hamilton Walk, Philadelphia, Pennsylvania 19104 6076, USA
    Nat Med 11:1287-9. 2005
    ..LEDGF is thus the first example of a cellular protein controlling the location of HIV integration in human cells...
  26. doi Identification of cellular factors binding to acetylated HIV-1 integrase
    Awatef Allouch
    Molecular Biology Laboratory, Scuola Normale Superiore, Pisa, Italy
    Amino Acids 41:1137-45. 2011
    ..Nevertheless, few two-hybrid hits bind with the same affinity the acetylated and the unmodified IN. These results further underlie the relevance of IN post-translational modification by acetylation in HIV-1 replication cycle...
  27. ncbi Lens epithelium-derived growth factor/p75 prevents proteasomal degradation of HIV-1 integrase
    Manuel Llano
    Molecular Medicine Program and Department of Immunology, Mayo Clinic College of Medicine, 200 First Street SW, Rochester, MN 55905, USA
    J Biol Chem 279:55570-7. 2004
    ..Protection requires only interaction with LEDGF/p75, and it is independent of the subcellular localization of the IN-LEDGF complex...
  28. ncbi Human immunodeficiency virus type 1 protease microinjected into cultured human skin fibroblasts cleaves vimentin and affects cytoskeletal and nuclear architecture
    B Höner
    Max Planck Institut für Zellbiologie, Ladenburg bei Heidelberg, Federal Republic of Germany
    J Cell Sci 100:799-807. 1991
    ..Thus, the HIV-1 PR is capable not only of cleaving IF subunit proteins in vivo, but also can catalyze alterations in other cellular structures...
  29. pmc LEDGF/p75 proteins with alternative chromatin tethers are functional HIV-1 cofactors
    Anne M Meehan
    Department of Molecular Medicine, Mayo Clinic College of Medicine, Rochester, Minnesota, USA
    PLoS Pathog 5:e1000522. 2009
    ..NDE mutants were corroborative. Chromatin tethering per se is a basic HIV-1 requirement and this rather than engagement of particular chromatin ligands is important for the LEDGF/p75 cofactor mechanism...
  30. ncbi Cleavage of eIF4G by HIV-1 protease: effects on translation
    Celia Perales
    Centro de Biologi a Molecular Severo Ochoa, Facultad de Ciencias, Universidad Autonoma CSIC, Cantoblanco 28049, Madrid, Spain
    FEBS Lett 533:89-94. 2003
    ..Our present findings suggest that eIF4GI intactness is necessary to maintain cap-dependent translation, not only in cell-free systems but also in mammalian cells...
  31. doi Targeting the protein-protein interactions of the HIV lifecycle
    Ali Tavassoli
    University of Southampton, Southampton, SO17 1BJ, UK
    Chem Soc Rev 40:1337-46. 2011
    ....
  32. pmc Inhibition of early stages of HIV-1 assembly by INI1/hSNF5 transdominant negative mutant S6
    Jennifer Cano
    Department of Genetics, Albert Einstein College of Medicine, Bronx, NY 1046, USA
    J Virol 85:2254-65. 2011
    ....
  33. pmc Apoptosis mediated by HIV protease is preceded by cleavage of Bcl-2
    P R Strack
    Molecular Biology Department, DuPont Merck Pharmaceutical Company, Wilmington, DE 19880 0336, USA
    Proc Natl Acad Sci U S A 93:9571-6. 1996
    ..Purified bcl-2 is cleaved by HIV protease between phenylalanine 112 and alanine 113. The results suggest a new option for HIV gene therapy; bcl-2 muteins that have noncleavable alterations surrounding the HIV protease cleavage site...
  34. pmc Human immunodeficiency virus type 1 protease cleaves the intermediate filament proteins vimentin, desmin, and glial fibrillary acidic protein
    R L Shoeman
    Max Planck Institut für Zellbiologie, Abteilung Traub, Rosenhof, Federal Republic of Germany
    Proc Natl Acad Sci U S A 87:6336-40. 1990
    ..Most commonly, the intermediate filaments collapsed into a clump with a juxtanuclear localization. These results support the possibility that intermediate filament proteins may serve as substrates within HIV-1-infected cells...
  35. pmc Design of HIV-1 integrase inhibitors targeting the catalytic domain as well as its interaction with LEDGF/p75: a scaffold hopping approach using salicylate and catechol groups
    Xing Fan
    State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 555 Zuchongzhi Road, Shanghai 201203, China
    Bioorg Med Chem 19:4935-52. 2011
    ..This work provides a new and efficient approach to evolve novel HIV-1 IN inhibitors from rational integration and optimization of previously reported inhibitors...
  36. pmc Overexpression of the lens epithelium-derived growth factor/p75 integrase binding domain inhibits human immunodeficiency virus replication
    Jan De Rijck
    Molecular Medicine, KU Leuven and IRC KULAK, Kapucijnenvoer 33 VCTB 5, B 3000 Leuven, Belgium
    J Virol 80:11498-509. 2006
    ..These data support LEDGF/p75 as an important cofactor for HIV replication and provide proof of concept for the LEDGF/p75-integrase interaction as a novel target for treating HIV-1 infection...
  37. pmc Design of HIV-1 protease inhibitors with C3-substituted hexahydrocyclopentafuranyl urethanes as P2-ligands: synthesis, biological evaluation, and protein-ligand X-ray crystal structure
    Arun K Ghosh
    Department of Chemistry, Purdue University, West Lafayette, Indiana 47907, USA
    J Med Chem 54:5890-901. 2011
    ..Inhibitor 26 exhibited potent activity against a panel of multidrug-resistant HIV-1 variants. A high resolution X-ray structure of 26-bound HIV-1 protease revealed important molecular insight into the ligand-binding site interactions...
  38. ncbi HIV integration. Ini1 for integration?
    M D Miller
    Infectious Disease Laboratory, Salk Institute, La Jolla, California 92037, USA
    Curr Biol 5:368-70. 1995
    ..The newly discovered Ini1 cellular protein binds HIV-1 integrase and is part of a protein complex thought to alter nucleosomal structure; such alterations may influence the selection of sites for HIV-1 DNA integration...
  39. ncbi Cellular co-factors of HIV-1 integration
    Bénédicte Van Maele
    Molecular Virology and Gene Therapy, Molecular Medicine, KULAK and K U Leuven, Kapucijnenvoer 33 3000 Leuven, Flanders, Belgium
    Trends Biochem Sci 31:98-105. 2006
    ..Although a direct role in integration has only been demonstrated for LEDGF/p75, to date, each validated cellular co-factor for HIV-1 integration could constitute a promising new target for antiviral therapy...
  40. ncbi Biochemical and genetic analyses of integrase-interacting proteins lens epithelium-derived growth factor (LEDGF)/p75 and hepatoma-derived growth factor related protein 2 (HRP2) in preintegration complex function and HIV-1 replication
    Nick Vandegraaff
    Department of Cancer Immunology and AIDS, Dana Farber Cancer Institute, Boston, MA 02115, USA
    Virology 346:415-26. 2006
    ..We conclude that cells knocked-out for LEDGF/p75 and/or HRP2 will be useful genetic tools to address the roles of these host cell factors in HIV-1 replication...
  41. ncbi Actin, troponin C, Alzheimer amyloid precursor protein and pro-interleukin 1 beta as substrates of the protease from human immunodeficiency virus
    A G Tomasselli
    Upjohn Laboratories, Upjohn Company, Kalamazoo, Michigan 49001
    J Biol Chem 266:14548-53. 1991
    ....
  42. ncbi Identification of the LEDGF/p75 HIV-1 integrase-interaction domain and NLS reveals NLS-independent chromatin tethering
    Maria Vanegas
    Molecular Medicine Program, Mayo Clinic College of Medicine, Rochester, MN 55905, USA
    J Cell Sci 118:1733-43. 2005
    ..LEDGF/p75 is a multidomain adaptor protein that interacts with the nuclear import apparatus, lentiviral IN proteins and chromatin by means of an NLS, an IBD and additional chromatin-interacting domains...
  43. doi A new functional role of HIV-1 integrase during uncoating of the viral core
    Marisa S Briones
    Department of Molecular and Medical Pharmacology, Molecular Biology Institute, 650 Charles E Young Drive, Los Angeles, CA 90095, USA
    Immunol Res 48:14-26. 2010
    ..Cyclophilin A and TRIM5α are two cellular factors that interact with CA in exerting their effects on HIV-1 replication. Here, we review the current understanding of uncoating and the new functional role of HIV-1 IN during uncoating...
  44. ncbi Involvement of HIV-1 protease in virus-induced cell killing
    Iván Ventoso
    Centro de Biologia Molecular Severo Ochoa, Facultad de Ciencias, Universidad Autonoma de Madrid, Cantoblanco, Madrid 28049, Spain
    Antiviral Res 66:47-55. 2005
    ..These findings may contribute towards a better understanding of HIV-1 pathogenesis, and emphasise the potential of the virus protease as a key therapeutic target in AIDS treatment...
  45. pmc Establishment of a functional human immunodeficiency virus type 1 (HIV-1) reverse transcription complex involves the cytoskeleton
    A Bukrinskaya
    Program in Molecular Medicine, University of Massachusetts Medical Center, Worcester, Massachusetts 01605, USA
    J Exp Med 188:2113-25. 1998
    ....
  46. doi Reverse two-hybrid screening to analyze protein-protein interaction of HIV-1 viral and cellular proteins
    Supratik Das
    Department of Molecular Genetics, Albert Einstein College of Medicine, Bronx, NY, USA
    Methods Mol Biol 485:271-93. 2009
    ..Once the mutants are isolated, they are tested in other biochemical systems and can be subjected to further functional and virological studies...
  47. doi Mutations at human immunodeficiency virus type 1 reverse transcriptase tryptophan repeat motif attenuate the inhibitory effect of efavirenz on virus production
    Chien Cheng Chiang
    Department of Medical Research and Education, Taipei Veterans General Hospital, Taiwan
    Virology 383:261-70. 2009
    ..Our results suggest that the Trp repeat motif may play a role in the Gag-Pol/Gag-Pol interaction that contributes to subsequent PR activation...
  48. pmc Structural basis for functional tetramerization of lentiviral integrase
    Stephen Hare
    Division of Medicine, St Mary s Campus, Imperial College London, London, United Kingdom
    PLoS Pathog 5:e1000515. 2009
    ..Our structures moreover highlight adaptable changes at the interfaces of individual IN dimers that allow divergent lentiviruses to utilize a highly-conserved, common integration co-factor...
  49. ncbi A cellular anti-apoptosis protein is cleaved by the HIV-1 protease
    B D Korant
    DuPont Merck Pharmaceutical Co, Experimental Station, Wilmington, Delaware 19880 0336, USA
    Adv Exp Med Biol 436:27-9. 1998
    ..The loss of bcl-2 has biological consequences, leading to enhanced HIV replication and programmed death of the host cell. A strategy is proposed to suppress HIV with non-cleavable mutants of bcl-2...
  50. pmc Transcription factor YY1 interacts with retroviral integrases and facilitates integration of moloney murine leukemia virus cDNA into the host chromosomes
    Yujin Inayoshi
    Department of Biotechnology, Graduate School of Engineering, Nagoya University, Furo cho, Chikusa ku, Nagoya 464 8603, Japan
    J Virol 84:8250-61. 2010
    ..Taking these results together, Yin Yang 1 appears to be involved in integration events during the early viral life cycle, possibly as an enhancer of integration...
  51. pmc Active nuclear import of human immunodeficiency virus type 1 preintegration complexes
    M I Bukrinsky
    Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha
    Proc Natl Acad Sci U S A 89:6580-4. 1992
    ....
  52. doi HIV-1 nucleocapsid traps reverse transcriptase on nucleic acid substrates
    Dina Grohmann
    Institut Gilbert Laustriat, Photophysique des interactions moleculaires, UMR 7175 CNRS, Faculte de Pharmacie, Universite Louis Pasteur, Strasbourg 1, 74, route du Rhin, 67401 Illkirch, France
    Biochemistry 47:12230-40. 2008
    ....
  53. doi HIV-1 reverse transcriptase can simultaneously engage its DNA/RNA substrate at both DNA polymerase and RNase H active sites: implications for RNase H inhibition
    Greg L Beilhartz
    Department of Microbiology and Immunology, McGill University, 3775 University Street, Montreal, Quebec, Canada
    J Mol Biol 388:462-74. 2009
    ..In conclusion, the bound nucleic acid substrate and in turn, active DNA synthesis can represent an obstacle to RNase H inhibition with compounds that bind to the RNase H active site...
  54. pmc The N348I mutation at the connection subdomain of HIV-1 reverse transcriptase decreases binding to nevirapine
    Matthew M Schuckmann
    Christopher Bond Life Sciences Center, Department of Molecular Microbiology and Immunology, University of Missouri School of Medicine, Columbia, Missouri 65211, USA
    J Biol Chem 285:38700-9. 2010
    ..In conclusion, connection subdomain mutation N348I decreases catalytic efficiency and causes in vitro resistance to NVP by decreasing inhibitor binding...
  55. pmc Biochemical and virological analysis of the 18-residue C-terminal tail of HIV-1 integrase
    Mohd J Dar
    Department of Cancer Immunology and AIDS, Dana Farber Cancer Institute, 44 Binney Street, Boston, MA, USA
    Retrovirology 6:94. 2009
    ..The F185K core domain mutation, which increases integrase protein solubility, was furthermore analyzed in a subset of mutants...
  56. pmc Role of human immunodeficiency virus type 1 integrase in uncoating of the viral core
    Marisa S Briones
    Department of Molecular and Medical Pharmacology, Molecular Biology Institute and UCLA AIDS Institute, UCLA School of Medicine, Los Angeles, California 90095, USA
    J Virol 84:5181-90. 2010
    ..Taken together, our results indicate that IN is required during uncoating for maintaining CypA-CA interaction, which promotes optimal stability of the viral core...
  57. ncbi Identification and characterization of the chromatin-binding domains of the HIV-1 integrase interactor LEDGF/p75
    Manuel Llano
    Molecular Medicine Program, Mayo Clinic College of Medicine, Rochester, MN 55905, USA
    J Mol Biol 360:760-73. 2006
    ..The results establish a molecular mechanism for LEDGF/p75-mediated tethering of HIV-1 integrase to chromatin...
  58. pmc Specificity of interaction of INI1/hSNF5 with retroviral integrases and its functional significance
    Eric Yung
    Department of Molecular Genetics, Albert Einstein College of Medicine, New York, New York 10461, USA
    J Virol 78:2222-31. 2004
    ..Taken together, these results suggest that INI1/hNSF5 is a host factor restricted for HIV-1 and that S6 acts as a highly specific and potent inhibitor of HIV-1 replication...
  59. pmc Identification and characterization of PWWP domain residues critical for LEDGF/p75 chromatin binding and human immunodeficiency virus type 1 infectivity
    Ming Chieh Shun
    Department of Cancer Immunology and AIDS, Dana Farber Cancer Institute and Division of AIDS, Harvard Medical School, Boston, MA 02115, USA
    J Virol 82:11555-67. 2008
    ..This initial systematic mutagenesis of a PWWP domain identifies amino acid residues critical for chromatin binding function and the consequences of their changes on HIV-1 integration and infection...
  60. pmc Chromosomal integration of LTR-flanked DNA in yeast expressing HIV-1 integrase: down regulation by RAD51
    S Desfarges
    UMR 5097 CNRS, Bordeaux, France
    Nucleic Acids Res 34:6215-24. 2006
    ..Our data allowed the identification of RAD51 as a novel in vitro IN cofactor able to down regulate the activity of this retroviral enzyme, thereby acting as a potential cellular restriction factor to HIV infection...
  61. pmc Nuclear import of HIV-1 intracellular reverse transcription complexes is mediated by importin 7
    Ariberto Fassati
    The Wohl Virion Centre, Windeyer Institute, University College London Medical School, 46 Cleveland Street, London W1T 4JF, UK
    EMBO J 22:3675-85. 2003
    ..These results provide a new insight into the molecular mechanism for HIV-1 nuclear import and reveal potential targets for therapeutic intervention...
  62. ncbi Inhibition of HIV-1 virion production by a transdominant mutant of integrase interactor 1
    E Yung
    Department of Molecular Genetics, Albert Einstein College of Medicine, Bronx, New York, USA
    Nat Med 7:920-6. 2001
    ....
  63. pmc LEDGF/p75 functions downstream from preintegration complex formation to effect gene-specific HIV-1 integration
    Ming Chieh Shun
    Department of Cancer Immunology and AIDS, Dana Farber Cancer Institute, Division of AIDS, Harvard Medical School, Boston, Massachusetts 02115, USA
    Genes Dev 21:1767-78. 2007
    ..Akin to yeast retrotransposons, disrupting the lentiviral targeting mechanism significantly perturbs overall integration...
  64. pmc Implication of serine residues 271, 273, and 275 in the human immunodeficiency virus type 1 cofactor activity of lens epithelium-derived growth factor/p75
    Jose A Garcia-Rivera
    Department of Biological Sciences, University of Texas at El Paso, 500 West University Ave, El Paso, TX 79968, USA
    J Virol 84:740-52. 2010
    ..These results clearly indicated that serine residues 271, 273, and 275 influence the HIV-1 cofactor activity of integrase-to-chromatin-tethering-competent LEDGF/p75...
  65. pmc Structural basis for HIV-1 DNA integration in the human genome, role of the LEDGF/P75 cofactor
    Fabrice Michel
    IGBMC Institut de Génétique et de Biologie Moléculaire et Cellulaire, Departement de Biologie et de Genomique Structurales, UDS, CNRS, INSERM, Illkirch, France
    EMBO J 28:980-91. 2009
    ..The positions of the target and viral DNAs for the 3' processing and integration reaction shed light on the integration mechanism, a process with wide implications for the understanding of viral-induced pathologies...
  66. pmc HIV-1 infection of nondividing cells through the recognition of integrase by the importin/karyopherin pathway
    P Gallay
    The Salk Institute, 10010 North Torrey Pines Road, La Jolla, CA 92037 1099, USA
    Proc Natl Acad Sci U S A 94:9825-30. 1997
    ..This novel function of integrase reflects the recognition of an atypical bipartite nuclear localization signal by the importin/karyopherin pathway...
  67. ncbi Topoisomerase I dissociates human immunodeficiency virus type 1 reverse transcriptase from genomic RNAs
    Hidehiro Takahashi
    Department of Pathology, National Institute of Infectious Diseases, Toyama 1 23 1, Shinjuku ku, Tokyo 162 8640, Japan
    Biochem Biophys Res Commun 313:1073-8. 2004
    ..We propose that human topoisomerase I may regulate the binding of RT to RNAs and play a pivotal role in HIV-1 replication...
  68. ncbi HIV-1 cDNA integration: requirement of HMG I(Y) protein for function of preintegration complexes in vitro
    C M Farnet
    Salk Institute for Biological Studies, La Jolla, California 92024, USA
    Cell 88:483-92. 1997
    ..Recombinant HMG I(Y) also complemented salt-stripped complexes. The finding that a host protein is required for integration by HIV PICs parallels findings in several well-studied transposition and site-specific recombination systems...
  69. pmc Functional interactions of human immunodeficiency virus type 1 integrase with human and yeast HSP60
    V Parissi
    REGER, UMR 5097 Centre National de la Recherche Scientifique CNRS Université Victor Segalen Bordeaux 2, Bordeaux, France
    J Virol 75:11344-53. 2001
    ..In addition, the functional human HSP60-HSP10 complex in the presence of ATP was able to recognize the HIV-1 IN as a substrate...
  70. ncbi Interaction of HIV-1 integrase with DNA repair protein hRad18
    Lubbertus C F Mulder
    Aaron Diamond AIDS Research Center, The Rockefeller University, New York, NY 10016, USA
    J Biol Chem 277:27489-93. 2002
    ..Taken together, these findings identify hRad18 as a novel interacting partner of HIV-1 integrase and suggest a role for post-replication/translesion DNA repair in the retroviral integration process...
  71. pmc Nuclear localization of human immunodeficiency virus type 1 preintegration complexes (PICs): V165A and R166A are pleiotropic integrase mutants primarily defective for integration, not PIC nuclear import
    Ana Limon
    Department of Pathology, Harvard Medical School, Dana Farber Cancer Institute, Boston, Massachusetts 02115, USA
    J Virol 76:10598-607. 2002
    ....
  72. doi AKAP149 binds to HIV-1 reverse transcriptase and is involved in the reverse transcription
    Julie Lemay
    Institut Cochin, Universite Paris Descartes, CNRS UMR 8104, Paris, France
    J Mol Biol 383:783-96. 2008
    ..This suggests that the interaction between RT and AKAP149 in infected cells may play an important role in HIV-1 reverse transcription...
  73. pmc Reassessment of the roles of integrase and the central DNA flap in human immunodeficiency virus type 1 nuclear import
    Jeffrey D Dvorin
    Department of Microbiology, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104 6148, USA
    J Virol 76:12087-96. 2002
    ..Thus, neither the cPPT nor the valine residue at position 165 of integrase is essential for the nuclear import of HIV-1 PICs...
  74. pmc HuR interacts with human immunodeficiency virus type 1 reverse transcriptase, and modulates reverse transcription in infected cells
    Julie Lemay
    Institut Cochin, Universite Paris Descartes, CNRS UMR8104, Paris, France
    Retrovirology 5:47. 2008
    ..These results suggest that HuR may be involved in and may modulate the reverse transcription reaction of HIV-1, by an as yet unknown mechanism involving a protein-protein interaction with HIV-1 RT...
  75. pmc Effect of the active site D25N mutation on the structure, stability, and ligand binding of the mature HIV-1 protease
    Jane M Sayer
    Laboratory of Chemical Physics, NIDDK, National Institutes of Health, Bethesda, Maryland 20892 0520, USA
    J Biol Chem 283:13459-70. 2008
    ..DRV by a factor of approximately 10(6) relative to PR.DRV. These results suggest that interactions mediated by the catalytic Asp residues make a major contribution to the tight binding of DRV to PR...
  76. pmc N348I in the connection domain of HIV-1 reverse transcriptase confers zidovudine and nevirapine resistance
    Soo Huey Yap
    Molecular Interactions Group, Macfarlane Burnet Institute for Medical Research and Public Health, Melbourne, Victoria, Australia
    PLoS Med 4:e335. 2007
    ..The objective of this multidisciplinary study was to establish the in vivo relevance of this mutation and its role in drug resistance...
  77. pmc The barrier-to-autointegration factor is a component of functional human immunodeficiency virus type 1 preintegration complexes
    Chou Wen Lin
    Department of Cancer Immunology and AIDS, Dana Farber Cancer Institute, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Virol 77:5030-6. 2003
    ..These data are consistent with the model that BAF functions as an integration cofactor in vivo...
  78. ncbi Reversion of the lethal phenotype of an HIV-1 integrase mutant virus by overexpression of the same integrase mutant protein
    Stephane Priet
    Pathogénie des Infections à Lentivirus, INSERM U372, 163 Avenue de Luminy, BP 178, 13276 Marseille Cedex 9, France
    J Biol Chem 278:20724-30. 2003
    ..This is the first study reporting that blockage of the integration process of HIV-1 provirus carrying a mutation of IN could be alleviated by increasing amounts of IN even carrying the same mutations...
  79. pmc Defining the DNA substrate binding sites on HIV-1 integrase
    James Dolan
    Department of Microbiology and Immunology, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USA
    J Mol Biol 385:568-79. 2009
    ..Mutations at seven other residues reported in the literature have the same phenotype, and all eight residues align along the length of the putative target DNA binding trench...
  80. ncbi Cytoplasmic recruitment of INI1 and PML on incoming HIV preintegration complexes: interference with early steps of viral replication
    P Turelli
    Department of Genetics and Microbiology, University of Geneva, CH 1211 4, Geneva, Switzerland
    Mol Cell 7:1245-54. 2001
    ..These results unveil a so far unsuspected cellular response that interferes with the early steps of HIV replication...
  81. pmc Role of the non-homologous DNA end joining pathway in the early steps of retroviral infection
    L Li
    Infectious Disease Laboratory, The Salk Institute, 10010 North Torrey Pines Road, La Jolla, CA 92037, USA
    EMBO J 20:3272-81. 2001
    ..We propose that the double-strand ends present in unintegrated cDNA promote apoptosis, as is known to be the case for chromosomal double-strand breaks, and cDNA circularization removes the pro-apoptotic signal...
  82. ncbi Thioltransferase (glutaredoxin) is detected within HIV-1 and can regulate the activity of glutathionylated HIV-1 protease in vitro
    D A Davis
    HIV and AIDS Malignancy Branch, NCI, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 272:25935-40. 1997
    ..Our results implicate thioltransferase in the regulation and/or maintenance of protease activity in HIV-1 infected cells...
  83. pmc HMG protein family members stimulate human immunodeficiency virus type 1 and avian sarcoma virus concerted DNA integration in vitro
    P Hindmarsh
    Department of Biochemistry, Case Western Reserve University School of Medicine, Cleveland, Ohio 44106 4935, USA
    J Virol 73:2994-3003. 1999
    ..While complexes containing HMG-I(Y), ASV IN, and donor DNA can be detected in gel shift experiments, coprecipitation experiments failed to demonstrate stable interactions between HMG-I(Y) and ASV IN or between HMG-I(Y) and HIV-1 IN...
  84. ncbi A role for DNA-PK in retroviral DNA integration
    R Daniel
    Institute for Cancer Research, Fox Chase Cancer Center, 7701 Burholme Avenue, Philadelphia, PA 19111, USA
    Science 284:644-7. 1999
    ..These results suggest that the initial events in retroviral integration are detected as DNA damage by the host cell and that completion of the integration process requires the DNA-PK-mediated repair pathway...
  85. ncbi Nuclear localization of human immunodeficiency virus type 1 integrase expressed as a fusion protein with green fluorescent protein
    W Pluymers
    Rega Institute for Medical Research, Katholieke Universiteit, Leuven, Belgium
    Virology 258:327-32. 1999
    ..Nuclear localization of GFP-IN was also obtained after transient transfection of the cells arrested in the G1/S phase of the cell cycle. These results provide compelling evidence for the karyophilic properties of the HIV-1 integrase...
  86. ncbi Identification of accessory mutations associated with high-level resistance in HIV-1 reverse transcriptase
    Patricia A Cane
    Centre for Infections, Health Protection Agency, UK
    AIDS 21:447-55. 2007
    ..To identify accessory mutations associated with high levels of resistance to reverse transcriptase inhibitors (RTI)...
  87. pmc Contribution of the C-terminal region within the catalytic core domain of HIV-1 integrase to yeast lethality, chromatin binding and viral replication
    Zaikun Xu
    Laboratory of Molecular Human Retrovirology, Department of Medical Microbiology, University of Manitoba, 508 730 William Avenue, Winnipeg, R3E 0W3, Canada
    Retrovirology 5:102. 2008
    ....
  88. pmc Modulation of human immunodeficiency virus type 1 protease autoprocessing by charge properties of surface residue 69
    Liangqun Huang
    Department of Biochemistry and Molecular Biology, Colorado State University, Fort Collins, CO 80523 1870, USA
    J Virol 83:7789-93. 2009
    ....
  89. pmc Wortmannin potentiates integrase-mediated killing of lymphocytes and reduces the efficiency of stable transduction by retroviruses
    R Daniel
    Fox Chase Cancer Center, Institute for Cancer Research, Philadelphia, Pennsylvania 19111, USA
    Mol Cell Biol 21:1164-72. 2001
    ..The studies also suggest that cells can be sensitized to such killing and stable retroviral DNA integration blocked by drugs that inhibit cellular DNA repair pathways...
  90. ncbi Characterization of the nuclear import pathway for HIV-1 integrase
    C Depienne
    Institut Jacques Monod, Unite Mixte de Recherche 7592, CNRS, Universite Paris VI, Universite Paris VII, Paris 75251, France
    J Biol Chem 276:18102-7. 2001
    ....
  91. ncbi HIV-1 infection requires a functional integrase NLS
    M Bouyac-Bertoia
    Department of Microbiology, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA
    Mol Cell 7:1025-35. 2001
    ....
  92. pmc Mechanism by which a glutamine to leucine substitution at residue 509 in the ribonuclease H domain of HIV-1 reverse transcriptase confers zidovudine resistance
    Jessica H Brehm
    Department of Medicine, Division of Infectious Diseases, University of Pittsburgh, Pittsburgh, Pennsylvania 15261, USA
    Biochemistry 47:14020-7. 2008
    ....
  93. ncbi Differential interaction of HIV-1 integrase and JPO2 with the C terminus of LEDGF/p75
    Koen Bartholomeeusen
    Molecular Medicine, KULeuven and IRC KULAK, Leuven, Belgium
    J Mol Biol 372:407-21. 2007
    ..Furthermore, JPO2 over-expression activated transcription from the HIV-1 LTR...
  94. pmc Evidence that the retroviral DNA integration process triggers an ATR-dependent DNA damage response
    Rene Daniel
    Institute for Cancer Research, Fox Chase Cancer Center, 7701 Burholme Avenue, Philadelphia, PA 19111, USA
    Proc Natl Acad Sci U S A 100:4778-83. 2003
    ..Components of the cellular DNA damage repair response may represent potential targets for antiretroviral drug development...
  95. ncbi Solution structure of the HIV-1 integrase-binding domain in LEDGF/p75
    Peter Cherepanov
    Department of Cancer Immunology and AIDS, Dana Farber Cancer Institute, 44 Binney Street, Boston, Massachusetts 02115, USA
    Nat Struct Mol Biol 12:526-32. 2005
    ..The initial structure determination of a host cell factor that tightly binds to a retroviral enzyme lays the groundwork for understanding enzyme-host interactions important for viral replication...
  96. ncbi Identification and characterization of a functional nuclear localization signal in the HIV-1 integrase interactor LEDGF/p75
    Goedele Maertens
    Department of Cancer Immunology and AIDS, Dana Farber Cancer Institute, Boston, Massachusetts 02115, USA
    J Biol Chem 279:33421-9. 2004
    ..Moreover, a single amino acid change in the NLS was sufficient to exclude the mutant LEDGF/p75 protein from the nucleus and abolish nuclear import of HIV-1 integrase...
  97. ncbi Structure-based mutagenesis of the integrase-LEDGF/p75 interface uncouples a strict correlation between in vitro protein binding and HIV-1 fitness
    Shaila Rahman
    Department of Cancer Immunology and AIDS, Dana Farber Cancer Institute, Boston, MA 02115, USA
    Virology 357:79-90. 2007
    ..Thus, the relative affinity of the in vitro IN-LEDGF/p75 interaction is not a universal predictor of IN mutant viral fitness...
  98. ncbi Inhibition of early steps of HIV-1 replication by SNF5/Ini1
    Marlène Maroun
    Institut Cochin, Département Maladies Infectieuses, F 75014 Paris, INSERM, U567, F 75014 Paris, France
    J Biol Chem 281:22736-43. 2006
    ..K71R substitution stimulates viral replication and results in higher infectious titers. Taken together, these results suggest that, by interacting with IN, SNF5/Ini1 interferes with early steps of HIV-1 infection...
  99. pmc Acetylation of HIV-1 integrase by p300 regulates viral integration
    Anna Cereseto
    Molecular Medicine Laboratory, International Centre for Genetic Engineering and Biotechnology ICGEB, Trieste, Italy
    EMBO J 24:3070-81. 2005
    ..This is the first demonstration that HIV-1 IN activity is specifically regulated by post-translational modification...
  100. pmc The human polycomb group EED protein interacts with the integrase of human immunodeficiency virus type 1
    Sebastien Violot
    Laboratoire de Virologie and Pathogénèse Virale, Faculté de médecine RTH Laennec, CNRS UMR 5537, France
    J Virol 77:12507-22. 2003
    ..i., suggesting the ocurrence of multiprotein complexes involving these three proteins at early steps of the HIV-1 virus life cycle. Such IEM patterns were not observed with a noninfectious, envelope deletion mutant of HIV-1...
  101. pmc LEDGF/p75 determines cellular trafficking of diverse lentiviral but not murine oncoretroviral integrase proteins and is a component of functional lentiviral preintegration complexes
    Manuel Llano
    Molecular Medicine Program, Mayo Clinic College of Medicine, Rochester, Minnesota 55905, USA
    J Virol 78:9524-37. 2004
    ..While lentiviral PIC nuclear import is unaffected by LEDGF/p75 knockdown, this protein is a component of functional lentiviral PICs. A role in HIV-1 integration site distribution merits investigation...

Research Grants82

  1. BIOCHEMICAL MECHANISM OF HIV DNA INTEGRATION
    Alan Engelman; Fiscal Year: 2004
    ..The results of these experiments will be used to formulate a detailed model of the structural and functional organization of HIV-1 PICs, which will aid the design of antiviral drugs targeted against HIV-1 integration. ..
  2. HIV-1 Preintegration Trafficking and Nuclear Localization
    Alan Engelman; Fiscal Year: 2007
    ..This knowledge in the long run will define novel targets for therapeutic interaction to develop new anti-viral drugs in the fight against HIV/AIDS. ..
  3. HIV-1 Integrase Structural Biology
    Alan Engelman; Fiscal Year: 2007
    ....
  4. MECHANISM OF NON-INTEGRASE-MEDIATED HIV-1 REPLICATION
    Alan Engelman; Fiscal Year: 2001
    ..Further aims are to develop gene therapy vectors based on non-integrating retroviruses. ..
  5. Nuclear Localization of HIV-1 Preintegration Complexes
    Alan Engelman; Fiscal Year: 2006
    ..abstract_text> ..
  6. LEDGF-Integrase Structural Biology
    Alan Engelman; Fiscal Year: 2005
    ..abstract_text> ..
  7. Nuclear Localization of HIV-1 Preintegration Complexes
    Alan Engelman; Fiscal Year: 2003
    ..abstract_text> ..
  8. BIOCHEMICAL MECHANISM OF HIV DNA INTEGRATION
    Alan Engelman; Fiscal Year: 2009
    ..abstract_text> ..
  9. HIV-1 Preintegration Trafficking and Nuclear Localization
    ALAN N ENGELMAN; Fiscal Year: 2010
    ..This knowledge in the long run will define novel targets for therapeutic interaction to develop new anti-viral drugs in the fight against HIV/AIDS. ..
  10. HIV-1 Integrase Structural Biology
    ALAN N ENGELMAN; Fiscal Year: 2010
    ..abstract_text> ..
  11. BIOCHEMICAL MECHANISM OF HIV DNA INTEGRATION
    Alan Engelman; Fiscal Year: 1999
    ..C. Examination of higher order protein-protein and protein-DNA interactions important for preintegration complex activity. D. Develop an assay for authentic integration of exogenous HIV-1 DNA. ..
  12. A human stem cell mouse model for HIV expression and inhibition
    Fatah Kashanchi; Fiscal Year: 2007
    ..Development of such a model would greatly contribute to the AIDS field when performing immunological, drug and vaccine studies. ..
  13. Development of the Gag/Lysyl tRNA synthetase interaction as a target for anti-HIV
    Lawrence Kleiman; Fiscal Year: 2010
    ....
  14. Protective cellular immune responses in HIV-1 discordant couples
    Carey Farquhar; Fiscal Year: 2007
    ....
  15. Effect of Cdk inhibitor on HIV-1 replication
    Fatah Kashanchi; Fiscal Year: 2007
    ..Therefore, the current proposal is aimed at determining whether drugs such as ATP analogs are a viable option in treating HIV-1 infected cells both in vitro and in vivo. ..
  16. Protective cellular immune responses in HIV-1 discordant couples
    Carey Farquhar; Fiscal Year: 2010
    ....
  17. CELL CYCLE REGULATED TRANSCRIPTION BY HIV1 TRANSACT TAT
    Fatah Kashanchi; Fiscal Year: 2002
    ..It is hoped that by understanding cell cycle events related to Tat activation, one might be able to predict similar findings in other viral activators, related to AIDs pathogenesis. ..
  18. HIV1 INHIBITION USING TAT PEPTIDE DERIVATIVES
    Fatah Kashanchi; Fiscal Year: 2002
    ..Studies are proposed to determine target(s), range and specificity of the core peptides in cell lines and PBMCs. ..
  19. HIV-1 INTEGRATION PROTEIN
    Duane Grandgenett; Fiscal Year: 2000
    ..Combinational inhibitor therapies against the rapid replication of HlV-1 in humans may be necessary to prevent AIDS. ..
  20. DESIGN AND SYNTHESIS OF NONPEPTIDE PROTEASE INHIBITORS
    Arun Ghosh; Fiscal Year: 2009
    ..This research integrates organic synthesis, protein-ligand x-ray crystallography, molecular modeling and in-depth virus and cell-biological studies to design the next generation of HIV-1 protease inhibitors. ..
  21. DESIGN AND SYNTHESIS OF NONPEPTIDE PROTEASE INHIBITORS
    Arun K Ghosh; Fiscal Year: 2010
    ..This research integrates organic synthesis, protein-ligand x-ray crystallography, molecular modeling and in-depth virus and cell-biological studies to design the next generation of HIV-1 protease inhibitors. ..
  22. DESIGN AND SYNTHESIS OF NONPEPTIDE PROTEASE INHIBITORS
    Arun Ghosh; Fiscal Year: 2009
    ..This research integrates organic synthesis, protein-ligand x-ray crystallography, molecular modeling and in-depth virus and cell-biological studies to design the next generation of HIV-1 protease inhibitors. ..
  23. DESIGN AND SYNTHESIS OF NONPEPTIDE PROTEASE INHIBITORS
    Arun Ghosh; Fiscal Year: 2001
    ....
  24. DESIGN AND SYNTHESIS OF NONPEPTIDE PROTEASE INHIBITORS
    Arun Ghosh; Fiscal Year: 2005
    ..Besides the broad range of scope and generality, this line of research will provide excellent opportunities to teach and train students in the laboratory. ..
  25. DEVELOPMENT OF LIGAND ASSISTED ASYMMETRIC SYNTHESES
    Arun Ghosh; Fiscal Year: 2001
    ..Besides the broad range of scope and generality, this line of research will provide excellent opportunities to teach and train graduate and undergraduate students in the laboratory. ..