Fyn

Summary

Gene Symbol: Fyn
Description: FYN proto-oncogene, Src family tyrosine kinase
Alias: SLK, SYN, p59-FYN, tyrosine-protein kinase Fyn, FYN oncogene related to SRC, FGR, YES, OKT3-induced calcium influx regulator, c-syn protooncogene, proto-oncogene Syn, proto-oncogene c-Fyn, src-like kinase, src/yes-related novel, tyrosine kinase p59fyn(T)
Species: human
Products:     Fyn

Top Publications

  1. Fukazawa T, Reedquist K, Trub T, Soltoff S, Panchamoorthy G, Druker B, et al. The SH3 domain-binding T cell tyrosyl phosphoprotein p120. Demonstration of its identity with the c-cbl protooncogene product and in vivo complexes with Fyn, Grb2, and phosphatidylinositol 3-kinase. J Biol Chem. 1995;270:19141-50 pubmed
    Previously, we have identified p120 as a Fyn/Lck SH3 and SH2 domain-binding protein that is tyrosine phosphorylated rapidly after T cell receptor triggering...
  2. Sarmady M, Dampier W, Tozeren A. Sequence- and interactome-based prediction of viral protein hotspots targeting host proteins: a case study for HIV Nef. PLoS ONE. 2011;6:e20735 pubmed publisher
    ..results match experimental data on binding sites of Nef to host proteins such as MAPK1, VAV1, LCK, HCK, HLA-A, CD4, FYN, and GNB2L1 with high statistical significance but is a poor predictor of Nef binding sites on highly flexible, ..
  3. Bunnell S, Henry P, Kolluri R, Kirchhausen T, Rickles R, Berg L. Identification of Itk/Tsk Src homology 3 domain ligands. J Biol Chem. 1996;271:25646-56 pubmed
    ..Initial studies with T cell lysates indicated that the Itk SH3 domain bound Cbl, Fyn, and other tyrosine phosphoproteins from TCR-stimulated Jurkat cells...
  4. Yaka R, Thornton C, Vagts A, Phamluong K, Bonci A, Ron D. NMDA receptor function is regulated by the inhibitory scaffolding protein, RACK1. Proc Natl Acad Sci U S A. 2002;99:5710-5 pubmed
    ..We found that RACK1 binds both the NR2B subunit of the NMDA receptor and the nonreceptor protein tyrosine kinase, Fyn. RACK1 inhibits Fyn phosphorylation of NR2B and decreases NMDA receptor-mediated currents in CA1 hippocampal slices...
  5. Chin J, Palop J, Puoliväli J, Massaro C, Bien Ly N, Gerstein H, et al. Fyn kinase induces synaptic and cognitive impairments in a transgenic mouse model of Alzheimer's disease. J Neurosci. 2005;25:9694-703 pubmed
    ..The tyrosine kinase Fyn is altered in Alzheimer's disease brains and modulates premature mortality and synaptotoxicity in hAPP mice...
  6. Marie Cardine A, Kirchgessner H, Schraven B. Molecular alterations of the Fyn-complex occur as late events of human T cell activation. Eur J Immunol. 1999;29:1175-87 pubmed
    ..lymphocytes resulted in the identification of an oligomeric protein complex which is constitutively formed between Fyn and several additional phosphoproteins (pp43, pp72, pp85, the protein tyrosine kinase Pyk2, as well as the two ..
  7. da Silva A, Li Z, de Vera C, Canto E, Findell P, Rudd C. Cloning of a novel T-cell protein FYB that binds FYN and SH2-domain-containing leukocyte protein 76 and modulates interleukin 2 production. Proc Natl Acad Sci U S A. 1997;94:7493-8 pubmed
    ..b>FYN binding protein (FYB or p120/130) associates with p59(fyn), the TcRzeta/CD3 complex, and becomes tyrosine-..
  8. Wary K, Mariotti A, Zurzolo C, Giancotti F. A requirement for caveolin-1 and associated kinase Fyn in integrin signaling and anchorage-dependent cell growth. Cell. 1998;94:625-34 pubmed
    Caveolin-1 functions as a membrane adaptor to link the integrin alpha subunit to the tyrosine kinase Fyn. Upon integrin ligation, Fyn is activated and binds, via its SH3 domain, to Shc...
  9. Mariotti A, Kedeshian P, Dans M, Curatola A, Gagnoux Palacios L, Giancotti F. EGF-R signaling through Fyn kinase disrupts the function of integrin alpha6beta4 at hemidesmosomes: role in epithelial cell migration and carcinoma invasion. J Cell Biol. 2001;155:447-58 pubmed
    ..Coimmunoprecipitation experiments indicate that Fyn and, to a lesser extent, Yes combine with alpha6beta4...

More Information

Publications67

  1. Seykora J, Mei L, Dotto G, Stein P. 'Srcasm: a novel Src activating and signaling molecule. J Biol Chem. 2002;277:2812-22 pubmed
    The Src family tyrosine kinase, Fyn, can facilitate regulation of cell proliferation and differentiation. Mice with mutations in the fyn gene have defects in the brain, immune system, and epidermal differentiation...
  2. Prasad K, Janssen O, Kapeller R, Raab M, Cantley L, Rudd C. Src-homology 3 domain of protein kinase p59fyn mediates binding to phosphatidylinositol 3-kinase in T cells. Proc Natl Acad Sci U S A. 1993;90:7366-70 pubmed
    ..In this study, we demonstrate that anti-CD3 and anti-Fyn immunoprecipitates possess PI 3-kinase activity as assessed by TLC and HPLC...
  3. Kim A, Jeon W, Lim K, Lee H, Kim W, Kim B. Fyn mediates transforming growth factor-beta1-induced down-regulation of E-cadherin in human A549 lung cancer cells. Biochem Biophys Res Commun. 2011;407:181-4 pubmed publisher
    ..However, the underlying molecular mechanisms are less well defined. We here show that Fyn, a member of Src family tyrosine kinases, plays a critical role in mediating TGF-?1-induced down-regulation of E-..
  4. Marie Cardine A, Kirchgessner H, Bruyns E, Shevchenko A, Mann M, Autschbach F, et al. SHP2-interacting transmembrane adaptor protein (SIT), a novel disulfide-linked dimer regulating human T cell activation. J Exp Med. 1999;189:1181-94 pubmed
    ..However, binding of SHP2 to SIT is not required for inhibition of NF-AT induction, suggesting that SIT not only regulates NF-AT activity but also controls NF-AT unrelated pathways of T cell activation involving SHP2. ..
  5. Sawada Y, Tamada M, Dubin Thaler B, Cherniavskaya O, Sakai R, Tanaka S, et al. Force sensing by mechanical extension of the Src family kinase substrate p130Cas. Cell. 2006;127:1015-26 pubmed
    ..Thus, we propose that Cas acts as a primary force sensor, transducing force into mechanical extension and thereby priming phosphorylation and activation of downstream signaling. ..
  6. Usardi A, Pooler A, Seereeram A, Reynolds C, Derkinderen P, Anderton B, et al. Tyrosine phosphorylation of tau regulates its interactions with Fyn SH2 domains, but not SH3 domains, altering the cellular localization of tau. FEBS J. 2011;278:2927-37 pubmed publisher
    ..demonstrated that interactions between the microtubule-associated protein tau and the nonreceptor tyrosine kinase Fyn play a critical role in mediating synaptic toxicity and neuronal loss in response to ?-amyloid (A?) in models of ..
  7. Garnett D, Barclay A, Carmo A, Beyers A. The association of the protein tyrosine kinases p56lck and p60fyn with the glycosyl phosphatidylinositol-anchored proteins Thy-1 and CD48 in rat thymocytes is dependent on the state of cellular activation. Eur J Immunol. 1993;23:2540-4 pubmed
    ..Additional phosphoproteins of 85, 36 and 32 kDa were consistently seen as components of the complexes. ..
  8. Suetsugu S, Hattori M, Miki H, Tezuka T, Yamamoto T, Mikoshiba K, et al. Sustained activation of N-WASP through phosphorylation is essential for neurite extension. Dev Cell. 2002;3:645-58 pubmed
    ..Collectively, neurite extension is regulated by the balance of N-WASP phosphorylation (activation) and degradation (inactivation), which are induced by tyrosine phosphorylation. ..
  9. Kim S, Kunapuli S. Negative regulation of Gq-mediated pathways in platelets by G(12/13) pathways through Fyn kinase. J Biol Chem. 2011;286:24170-9 pubmed publisher
    ..Importantly, AYPGKF-induced platelet aggregation and PKC activation were potentiated in Fyn-deficient but not in Lyn-deficient mice compared with wild-type littermates...
  10. Bhaskar K, Yen S, Lee G. Disease-related modifications in tau affect the interaction between Fyn and Tau. J Biol Chem. 2005;280:35119-25 pubmed
    ..We have recently shown that tau interacts with the SH3 domain of Fyn, an Src family non-receptor tyrosine kinase, and is tyrosine-phosphorylated by Fyn on Tyr-18...
  11. da Silva A, Janssen O, Rudd C. T cell receptor zeta/CD3-p59fyn(T)-associated p120/130 binds to the SH2 domain of p59fyn(T). J Exp Med. 1993;178:2107-13 pubmed
    ..this study, we have shown that this interaction with p120/130 is specifically mediated by the SH2 domain (not the fyn-SH3 domain) of p59fyn(T)...
  12. Bruyns E, Marie Cardine A, Kirchgessner H, Sagolla K, Shevchenko A, Mann M, et al. T cell receptor (TCR) interacting molecule (TRIM), a novel disulfide-linked dimer associated with the TCR-CD3-zeta complex, recruits intracellular signaling proteins to the plasma membrane. J Exp Med. 1998;188:561-75 pubmed
    ..Thus, TRIM represents a TCR-associated transmembrane adaptor protein which is likely involved in targeting of intracellular signaling proteins to the plasma membrane after triggering of the TCR. ..
  13. Kinnunen T, Kaksonen M, Saarinen J, Kalkkinen N, Peng H, Rauvala H. Cortactin-Src kinase signaling pathway is involved in N-syndecan-dependent neurite outgrowth. J Biol Chem. 1998;273:10702-8 pubmed
    ..Western blotting reveals both c-Src and Fyn in the active fractions...
  14. Arold S, Franken P, Strub M, Hoh F, Benichou S, Benarous R, et al. The crystal structure of HIV-1 Nef protein bound to the Fyn kinase SH3 domain suggests a role for this complex in altered T cell receptor signaling. Structure. 1997;5:1361-72 pubmed
    ..core of HIV-1 Nef protein alone and in complex with the wild-type SH3 domain of the p59fyn protein tyrosine kinase (Fyn), at 3.0 A resolution...
  15. van t Hof W, Resh M. Rapid plasma membrane anchoring of newly synthesized p59fyn: selective requirement for NH2-terminal myristoylation and palmitoylation at cysteine-3. J Cell Biol. 1997;136:1023-35 pubmed
    ..or NIH 3T3 cells with [35S]methionine followed by analysis of cytosolic and total membrane fractions showed that Fyn became membrane bound within 5 min after biosynthesis...
  16. Kawakami T, Pennington C, Robbins K. Isolation and oncogenic potential of a novel human src-like gene. Mol Cell Biol. 1986;6:4195-201 pubmed
    ..Nucleotide sequence analysis revealed that this gene, termed slk, encoded a 537-residue protein which was 86% identical to the chicken proto-oncogene product, p60c-src, over a ..
  17. Macdonald A, Mazaleyrat S, McCormick C, Street A, Burgoyne N, Jackson R, et al. Further studies on hepatitis C virus NS5A-SH3 domain interactions: identification of residues critical for binding and implications for viral RNA replication and modulation of cell signalling. J Gen Virol. 2005;86:1035-44 pubmed
    ..It is more likely that they play a role in altering the cellular environment to favour viral persistence. ..
  18. Salmond R, Filby A, Qureshi I, Caserta S, Zamoyska R. T-cell receptor proximal signaling via the Src-family kinases, Lck and Fyn, influences T-cell activation, differentiation, and tolerance. Immunol Rev. 2009;228:9-22 pubmed publisher
    ..Activation of the src-family kinases p56(lck) (Lck) and p59(fyn) (Fyn) is central to the initiation of TCR signaling pathways...
  19. Werdich X, Penn J. Src, Fyn and Yes play differential roles in VEGF-mediated endothelial cell events. Angiogenesis. 2005;8:315-26 pubmed
    Widely coexpressed Src family kinase (SFK) members Src, Fyn and Yes are involved in various cellular events, often acting downstream of receptor tyrosine kinases, such as vascular endothelial growth factor (VEGF) receptors...
  20. Lee G, Newman S, Gard D, Band H, Panchamoorthy G. Tau interacts with src-family non-receptor tyrosine kinases. J Cell Sci. 1998;111 ( Pt 21):3167-77 pubmed
    ..Here we report that a proline rich sequence in the amino terminus of tau interacts with the SH3 domains of fyn and src non-receptor tyrosine kinases...
  21. Shelton H, Harris M. Hepatitis C virus NS5A protein binds the SH3 domain of the Fyn tyrosine kinase with high affinity: mutagenic analysis of residues within the SH3 domain that contribute to the interaction. Virol J. 2008;5:24 pubmed publisher
    ..coli and examined their interactions with the SH3 domain of the Src-family tyrosine kinase, Fyn. Surface plasmon resonance analysis revealed that NS5A binds to the Fyn SH3 domain with what can be considered a ..
  22. Bunnell S, Diehn M, Yaffe M, Findell P, Cantley L, Berg L. Biochemical interactions integrating Itk with the T cell receptor-initiated signaling cascade. J Biol Chem. 2000;275:2219-30 pubmed
    ..Together, these observations suggest that multivalent interactions recruit Itk to LAT-nucleated signaling complexes and facilitate the activation of LAT-associated phospholipase Cgamma1 by Itk. ..
  23. Gorska M, Stafford S, Cen O, Sur S, Alam R. Unc119, a novel activator of Lck/Fyn, is essential for T cell activation. J Exp Med. 2004;199:369-79 pubmed
    ..step in T cell receptor for antigen (TCR) signaling is the activation of the receptor-bound Src kinases, Lck and Fyn. The exact mechanism of this process is unknown...
  24. Saito Y, Jensen A, Salgia R, Posadas E. Fyn: a novel molecular target in cancer. Cancer. 2010;116:1629-37 pubmed publisher
    b>Fyn is 59-kDa member of the Src family of kinases that is historically associated with T-cell and neuronal signaling in development and normal cellular physiology...
  25. Marie Cardine A, Kirchgessner H, Eckerskorn C, Meuer S, Schraven B. Human T lymphocyte activation induces tyrosine phosphorylation of alpha-tubulin and its association with the SH2 domain of the p59fyn protein tyrosine kinase. Eur J Immunol. 1995;25:3290-7 pubmed
    ..Among several proteins which bound to the fyn SH2 domain in lysates of human Jurkat T lymphocytes, alpha- and beta-tubulin were identified by N-terminal ..
  26. Yasuda K, Nagafuku M, Shima T, Okada M, Yagi T, Yamada T, et al. Cutting edge: Fyn is essential for tyrosine phosphorylation of Csk-binding protein/phosphoprotein associated with glycolipid-enriched microdomains in lipid rafts in resting T cells. J Immunol. 2002;169:2813-7 pubmed
    ..By analyzing T cells from Fyn-/- mice, we clearly demonstrated that Fyn, but not Lck, has its kinase activity in membrane rafts, and plays a ..
  27. Huang J, Tilly D, Altman A, Sugie K, Grey H. T-cell receptor antagonists induce Vav phosphorylation by selective activation of Fyn kinase. Proc Natl Acad Sci U S A. 2000;97:10923-9 pubmed
    ..In this study, we investigated the role played by the protein tyrosine kinases Fyn, Lck, and ZAP-70 in antagonist-induced signaling pathway...
  28. Piedra J, Miravet S, Castaño J, Palmer H, Heisterkamp N, Garcia de Herreros A, et al. p120 Catenin-associated Fer and Fyn tyrosine kinases regulate beta-catenin Tyr-142 phosphorylation and beta-catenin-alpha-catenin Interaction. Mol Cell Biol. 2003;23:2287-97 pubmed
    ..This residue can be phosphorylated in vitro by Fer or Fyn tyrosine kinases. Transfection of these kinases to epithelial cells disrupted the association between both catenins...
  29. Franco M, Furstoss O, Simon V, Benistant C, Hong W, Roche S. The adaptor protein Tom1L1 is a negative regulator of Src mitogenic signaling induced by growth factors. Mol Cell Biol. 2006;26:1932-47 pubmed
    ..is overcome by c-myc expression or p53 inactivation, two regulators of SFK mitogenic function; (iii) Src or Fyn coexpression overrides Tom1L1 mitogenic activity; (iv) overexpression of the adaptor reduces Src association with ..
  30. Mondragón Rodríguez S, Trillaud Doppia E, Dudilot A, Bourgeois C, Lauzon M, Leclerc N, et al. Interaction of endogenous tau protein with synaptic proteins is regulated by N-methyl-D-aspartate receptor-dependent tau phosphorylation. J Biol Chem. 2012;287:32040-53 pubmed publisher
    ..activation leads to a selective phosphorylation of specific sites in tau, regulating the interaction of tau with Fyn and the PSD95-NMDA receptor complex...
  31. Macdonald A, Crowder K, Street A, McCormick C, Harris M. The hepatitis C virus NS5A protein binds to members of the Src family of tyrosine kinases and regulates kinase activity. J Gen Virol. 2004;85:721-9 pubmed
    ..binding assays and co-immunoprecipitation experiments revealed an interaction between NS5A and Hck, Lck, Lyn and Fyn, but interestingly not Src itself...
  32. Gassmann M, Guttinger M, Amrein K, Burn P. Protein tyrosine kinase p59fyn is associated with the T cell receptor-CD3 complex in functional human lymphocytes. Eur J Immunol. 1992;22:283-6 pubmed
  33. Parravicini V, Gadina M, Kovarova M, Odom S, Gonzalez Espinosa C, Furumoto Y, et al. Fyn kinase initiates complementary signals required for IgE-dependent mast cell degranulation. Nat Immunol. 2002;3:741-8 pubmed
    ..We report here that Fc epsilon RI also uses a Fyn kinase-dependent pathway that does not require Lyn kinase or the adapter LAT for its initiation, but is necessary ..
  34. Semba K, Nishizawa M, Miyajima N, Yoshida M, Sukegawa J, Yamanashi Y, et al. yes-related protooncogene, syn, belongs to the protein-tyrosine kinase family. Proc Natl Acad Sci U S A. 1986;83:5459-63 pubmed
    An src/yes-related novel gene named syn (SYN in human gene nomenclature) has been identified in the human genome on chromosome 6 and characterized by molecular cloning...
  35. Waltenberger J, Claesson Welsh L, Siegbahn A, Shibuya M, Heldin C. Different signal transduction properties of KDR and Flt1, two receptors for vascular endothelial growth factor. J Biol Chem. 1994;269:26988-95 pubmed
    ..Members of the Src family such as Fyn and Yes showed an increased level of phosphorylation upon VEGF stimulation of cells expressing Flt1 but not in ..
  36. Gotoh N, Toyoda M, Shibuya M. Tyrosine phosphorylation sites at amino acids 239 and 240 of Shc are involved in epidermal growth factor-induced mitogenic signaling that is distinct from Ras/mitogen-activated protein kinase activation. Mol Cell Biol. 1997;17:1824-31 pubmed
    ..These results suggest that Shc activates two distinct signaling pathways, Y317 to Ras/MAPK and Y239 and Y240 to another pathway including Myc, and that both are involved in EGF-induced mitogenic signaling. ..
  37. Lee C, Saksela K, Mirza U, Chait B, Kuriyan J. Crystal structure of the conserved core of HIV-1 Nef complexed with a Src family SH3 domain. Cell. 1996;85:931-42 pubmed
    ..crystal structure of the conserved core of HIV-1 Nef has been determined in complex with the SH3 domain of a mutant Fyn tyrosine kinase (a single amino acid substitution, Arg-96 to isoleucine), to which Nef binds tightly...
  38. Banin S, Truong O, Katz D, Waterfield M, Brickell P, Gout I. Wiskott-Aldrich syndrome protein (WASp) is a binding partner for c-Src family protein-tyrosine kinases. Curr Biol. 1996;6:981-8 pubmed
    ..expressed in insect cells using a baculovirus vector demonstrated a specific interaction between WASp and the Fyn protein-tyrosine kinase...
  39. Chen Y, Chen C, Tung P, Huang S, Wang S. An acidic extracellular pH disrupts adherens junctions in HepG2 cells by Src kinases-dependent modification of E-cadherin. J Cell Biochem. 2009;108:851-9 pubmed publisher
    ..pH 6.6 treatment activated c-Src and Fyn kinase and induced tyrosine phosphorylation of p120 catenin (p120ctn) and E-cadherin...
  40. Han J, Das B, Wei W, Van Aelst L, Mosteller R, Khosravi Far R, et al. Lck regulates Vav activation of members of the Rho family of GTPases. Mol Cell Biol. 1997;17:1346-53 pubmed
    ..Further, we present data indicating that the Lck kinase activates the guanine nucleotide exchange factor and transforming activity of Vav. ..
  41. Saksela K, Cheng G, Baltimore D. Proline-rich (PxxP) motifs in HIV-1 Nef bind to SH3 domains of a subset of Src kinases and are required for the enhanced growth of Nef+ viruses but not for down-regulation of CD4. EMBO J. 1995;14:484-91 pubmed
    ..Thus, CD4 down-regulation and promotion of viral growth are two distinct functions of Nef, and the latter is mediated via SH3 binding. ..
  42. Katagiri T, Takahashi T, Sasaki T, Nakamura S, Hattori S. Protein-tyrosine kinase Pyk2 is involved in interleukin-2 production by Jurkat T cells via its tyrosine 402. J Biol Chem. 2000;275:19645-52 pubmed
    ..Furthermore, we found that Pyk2, when overexpressed, associates with Zap70 and Vav. Taken together, these findings suggest that Pyk2 is involved in the activation of T cells through its tyrosine 402. ..
  43. Marie Cardine A, Bruyns E, Eckerskorn C, Kirchgessner H, Meuer S, Schraven B. Molecular cloning of SKAP55, a novel protein that associates with the protein tyrosine kinase p59fyn in human T-lymphocytes. J Biol Chem. 1997;272:16077-80 pubmed
    In human T-lymphocytes the Src family protein tyrosine kinase p59(fyn) associates with three phosphoproteins of 43, 55, and 85 kDa (pp43, pp55, and pp85)...
  44. Derkinderen P, Scales T, Hanger D, Leung K, Byers H, Ward M, et al. Tyrosine 394 is phosphorylated in Alzheimer's paired helical filament tau and in fetal tau with c-Abl as the candidate tyrosine kinase. J Neurosci. 2005;25:6584-93 pubmed
    ..and tau from neurofibrillary tangles, Tyr-18 was reported to be a site of phosphorylation by the Src-family kinase Fyn. We examined whether tyrosine residues other than Tyr-18 are phosphorylated in tau and whether other tyrosine ..
  45. Cheng G, Ye Z, Baltimore D. Binding of Bruton's tyrosine kinase to Fyn, Lyn, or Hck through a Src homology 3 domain-mediated interaction. Proc Natl Acad Sci U S A. 1994;91:8152-5 pubmed
    ..Here, we show that Btk interacts with Src homology 3 domains of Fyn, Lyn, and Hck, protein-tyrosine kinases that get activated upon stimulation of B- and T-cell receptors...
  46. Tsygankov A, Mahajan S, Fincke J, Bolen J. Specific association of tyrosine-phosphorylated c-Cbl with Fyn tyrosine kinase in T cells. J Biol Chem. 1996;271:27130-7 pubmed
    b>Fyn is a Src family protein-tyrosine kinase functionally associated with the T-cell antigen receptor (TcR)/CD3 receptor complex...
  47. Collin G, Franco M, Simon V, Benistant C, Roche S. The Tom1L1-clathrin heavy chain complex regulates membrane partitioning of the tyrosine kinase Src required for mitogenic and transforming activities. Mol Cell Biol. 2007;27:7631-40 pubmed
    ..We concluded that the Tom1L1-CHC complex defines a novel mechanism involved in negative regulation of mitogenic and transforming signals, by modulating SFK partitioning at the plasma membrane. ..
  48. Lebouvier T, Scales T, Williamson R, Noble W, Duyckaerts C, Hanger D, et al. The microtubule-associated protein tau is also phosphorylated on tyrosine. J Alzheimers Dis. 2009;18:1-9 pubmed publisher
    ..In contrast, there is mounting evidence suggesting that tau tyrosine phosphorylation is an early event in the pathophysiology of AD and that Fyn and c-Abl are critical in the neurodegenerative process which occurs in tauopathies.
  49. Vila J, Gimferrer I, Padilla O, Arman M, Places L, Simarro M, et al. Residues Y429 and Y463 of the human CD5 are targeted by protein tyrosine kinases. Eur J Immunol. 2001;31:1191-8 pubmed
    ..This is in agreement with data from direct in vitro kinase assays using purified recombinant Lck and Fyn protein tyrosine kinases...
  50. da Silva A, Yamamoto M, Zalvan C, Rudd C. Engagement of the TcR/CD3 complex stimulates p59fyn(T) activity: detection of associated proteins at 72 and 120-130 kD. Mol Immunol. 1992;29:1417-25 pubmed
    ..using membrane vesicles demonstrated an anti-CD3 mediated induced increase (2-5-fold) in the phosphorylation of the fyn kinase...
  51. Grabulovski D, Kaspar M, Neri D. A novel, non-immunogenic Fyn SH3-derived binding protein with tumor vascular targeting properties. J Biol Chem. 2007;282:3196-204 pubmed
    ..Here, we describe the design, construction, characterization, and use of a novel human Fyn SH3 phage library, containing 1.2 x 10(9) individual clone members...
  52. Qian D, Lev S, van Oers N, Dikic I, Schlessinger J, Weiss A. Tyrosine phosphorylation of Pyk2 is selectively regulated by Fyn during TCR signaling. J Exp Med. 1997;185:1253-9 pubmed
    The Src family protein tyrosine kinases (PTKs), Lck and Fyn, are coexpressed in T cells and perform crucial functions involved in the initiation of T cell antigen receptor (TCR) signal transduction...
  53. Brdicka T, Pavlistová D, Leo A, Bruyns E, Korinek V, Angelisova P, et al. Phosphoprotein associated with glycosphingolipid-enriched microdomains (PAG), a novel ubiquitously expressed transmembrane adaptor protein, binds the protein tyrosine kinase csk and is involved in regulation of T cell activation. J Exp Med. 2000;191:1591-604 pubmed
    ..These findings collectively suggest that in the absence of external stimuli, the PAG-Csk complex transmits negative regulatory signals and thus may help to keep resting T cells in a quiescent state. ..
  54. Cicmil M, Thomas J, Sage T, Barry F, Leduc M, Bon C, et al. Collagen, convulxin, and thrombin stimulate aggregation-independent tyrosine phosphorylation of CD31 in platelets. Evidence for the involvement of Src family kinases. J Biol Chem. 2000;275:27339-47 pubmed
    ..In accordance with this, each of the Src family members expressed in platelets, namely Fyn, Lyn, Src, Yes, and Hck, was shown to co-immunoprecipitate with CD31...
  55. Lee C, Leung B, Lemmon M, Zheng J, Cowburn D, Kuriyan J, et al. A single amino acid in the SH3 domain of Hck determines its high affinity and specificity in binding to HIV-1 Nef protein. EMBO J. 1995;14:5006-15 pubmed
    We have examined the differential binding of Hck and Fyn to HIV-1 Nef to elucidate the structural basis of SH3 binding affinity and specificity...
  56. Arold S, Ulmer T, Mulhern T, Werner J, Ladbury J, Campbell I, et al. The role of the Src homology 3-Src homology 2 interface in the regulation of Src kinases. J Biol Chem. 2001;276:17199-205 pubmed
    ..approach involving crystallography, NMR, and isothermal titration calorimetry to study the regulatory fragment of Fyn (FynSH32) and its interaction with a physiological activator: a fragment of focal adhesion kinase that contains ..
  57. Mulhern T, Shaw G, Morton C, Day A, Campbell I. The SH2 domain from the tyrosine kinase Fyn in complex with a phosphotyrosyl peptide reveals insights into domain stability and binding specificity. Structure. 1997;5:1313-23 pubmed
    ..b>Fyn is a member of the Src family of tyrosine kinases that are involved in signal transduction by association with a ..
  58. Solheim S, Torgersen K, Tasken K, Berge T. Regulation of FynT function by dual domain docking on PAG/Cbp. J Biol Chem. 2008;283:2773-83 pubmed
    ..Thus, in T-cells, PAG is involved in positive as well as negative regulation of FynT activity. ..