FANCD2

Summary

Gene Symbol: FANCD2
Description: Fanconi anemia complementation group D2
Alias: FA-D2, FA4, FACD, FAD, FAD2, FANCD, Fanconi anemia, complementation group D2
Species: human

Top Publications

  1. ncbi The intraflavin hydrogen bond in human electron transfer flavoprotein modulates redox potentials and may participate in electron transfer
    T M Dwyer
    Department of Pediatrics, Cell and Developmental Biology Program, University of Colorado School of Medicine, Denver 80262, USA
    Biochemistry 38:9735-45. 1999
  2. pmc Human Fanconi anemia monoubiquitination pathway promotes homologous DNA repair
    Koji Nakanishi
    Molecular Biology Program, Memorial Sloan Kettering Cancer Center, 1275 York Avenue, New York, NY 10021, USA
    Proc Natl Acad Sci U S A 102:1110-5. 2005
  3. pmc Recruitment of DNA polymerase eta by FANCD2 in the early response to DNA damage
    Dechen Fu
    University of Hawaii Cancer Center UHCC, University of Hawaii, Honolulu, HI, USA
    Cell Cycle 12:803-9. 2013
  4. pmc Structural analysis of human FANCL, the E3 ligase in the Fanconi anemia pathway
    Charlotte Hodson
    Protein Structure and Function Laboratory, Lincoln s Inn Fields Laboratories of the London Research Institute, Cancer Research UK, 44 Lincoln s Inn Fields, London WC2A 3LY, United Kingdom
    J Biol Chem 286:32628-37. 2011
  5. ncbi S-phase-specific interaction of the Fanconi anemia protein, FANCD2, with BRCA1 and RAD51
    Toshiyasu Taniguchi
    Department of Pediatric Oncology, Dana Farber Cancer Institute, Harvard Medical School, Boston, MA 02115, USA
    Blood 100:2414-20. 2002
  6. ncbi Interaction of the Fanconi anemia proteins and BRCA1 in a common pathway
    I Garcia-Higuera
    Department of Pediatric Oncology, Dana Farber Cancer Institute, and Department of Pediatrics, Children s Hospital, Harvard Medical School, Boston, MA 02115, USA
    Mol Cell 7:249-62. 2001
  7. pmc FANCI protein binds to DNA and interacts with FANCD2 to recognize branched structures
    Fenghua Yuan
    Department of Biochemistry and Molecular Biology, University of Miami Miller School of Medicine, Miami, Florida 33136, USA
    J Biol Chem 284:24443-52. 2009
  8. pmc Significance of the Fanconi anemia FANCD2 protein in sporadic and metastatic human breast cancer
    Philip S Rudland
    Cancer and Polio Research Fund Laboratories, School of Biological Sciences, Biosciences Building, Crown Street, University of Liverpool, Liverpool, L69 3BX, United Kingdom
    Am J Pathol 176:2935-47. 2010
  9. ncbi FANCD2 protein is expressed in proliferating cells of human tissues that are cancer-prone in Fanconi anaemia
    Michael Hölzel
    Department of Pathology, VU University Medical Center, Amsterdam, The Netherlands
    J Pathol 201:198-203. 2003
  10. pmc DNA robustly stimulates FANCD2 monoubiquitylation in the complex with FANCI
    Koichi Sato
    Laboratory of Structural Biology, Graduate School of Advanced Science and Engineering, Waseda University, 2 2 Wakamatsu cho, Shinjuku ku, Tokyo 162 8480, Japan
    Nucleic Acids Res 40:4553-61. 2012

Detail Information

Publications211 found, 100 shown here

  1. ncbi The intraflavin hydrogen bond in human electron transfer flavoprotein modulates redox potentials and may participate in electron transfer
    T M Dwyer
    Department of Pediatrics, Cell and Developmental Biology Program, University of Colorado School of Medicine, Denver 80262, USA
    Biochemistry 38:9735-45. 1999
    ..denitrificans ETFs determined by X-ray crystallography indicate that the 4'-hydroxyl of the ribityl side chain of FAD is hydrogen bonded to N(1) of the flavin ring...
  2. pmc Human Fanconi anemia monoubiquitination pathway promotes homologous DNA repair
    Koji Nakanishi
    Molecular Biology Program, Memorial Sloan Kettering Cancer Center, 1275 York Avenue, New York, NY 10021, USA
    Proc Natl Acad Sci U S A 102:1110-5. 2005
    ..DNA-damaging agents activate the monoubiquitination of FANCD2, resulting in its targeting to nuclear foci that also contain BRCA1 and BRCA2/FANCD1, proteins involved in ..
  3. pmc Recruitment of DNA polymerase eta by FANCD2 in the early response to DNA damage
    Dechen Fu
    University of Hawaii Cancer Center UHCC, University of Hawaii, Honolulu, HI, USA
    Cell Cycle 12:803-9. 2013
    How Fanconi anemia (FA) protein D2 (FANCD2) performs DNA damage repair remains largely elusive. We report here that translesion synthesis DNA polymerase (pol) eta is a novel mediator of FANCD2 function...
  4. pmc Structural analysis of human FANCL, the E3 ligase in the Fanconi anemia pathway
    Charlotte Hodson
    Protein Structure and Function Laboratory, Lincoln s Inn Fields Laboratories of the London Research Institute, Cancer Research UK, 44 Lincoln s Inn Fields, London WC2A 3LY, United Kingdom
    J Biol Chem 286:32628-37. 2011
    ..At the heart of this pathway is the monoubiquitination of the FANCI-FANCD2 (ID) complex by the multiprotein "core complex" containing the E3 ubiquitin ligase FANCL...
  5. ncbi S-phase-specific interaction of the Fanconi anemia protein, FANCD2, with BRCA1 and RAD51
    Toshiyasu Taniguchi
    Department of Pediatric Oncology, Dana Farber Cancer Institute, Harvard Medical School, Boston, MA 02115, USA
    Blood 100:2414-20. 2002
    ..DNA damage activates this pathway, leading to monoubiquitination of the downstream FANCD2 protein and targeting to nuclear foci containing BRCA1...
  6. ncbi Interaction of the Fanconi anemia proteins and BRCA1 in a common pathway
    I Garcia-Higuera
    Department of Pediatric Oncology, Dana Farber Cancer Institute, and Department of Pediatrics, Children s Hospital, Harvard Medical School, Boston, MA 02115, USA
    Mol Cell 7:249-62. 2001
    ..a nuclear complex containing the FANCA, FANCC, FANCF, and FANCG proteins is required for the activation of the FANCD2 protein to a monoubiquitinated isoform...
  7. pmc FANCI protein binds to DNA and interacts with FANCD2 to recognize branched structures
    Fenghua Yuan
    Department of Biochemistry and Molecular Biology, University of Miami Miller School of Medicine, Miami, Florida 33136, USA
    J Biol Chem 284:24443-52. 2009
    ..When co-expressed in insect cells, a small fraction of FANCI forms a stable complex with FANCD2 (Fanconi anemia complementation group D2)...
  8. pmc Significance of the Fanconi anemia FANCD2 protein in sporadic and metastatic human breast cancer
    Philip S Rudland
    Cancer and Polio Research Fund Laboratories, School of Biological Sciences, Biosciences Building, Crown Street, University of Liverpool, Liverpool, L69 3BX, United Kingdom
    Am J Pathol 176:2935-47. 2010
    b>FANCD2, a pivotal protein in the Fanconi anemia and BRCA pathway/network, is monoubiquitylated in the nucleus in response to DNA damage...
  9. ncbi FANCD2 protein is expressed in proliferating cells of human tissues that are cancer-prone in Fanconi anaemia
    Michael Hölzel
    Department of Pathology, VU University Medical Center, Amsterdam, The Netherlands
    J Pathol 201:198-203. 2003
    ..At least seven distinct FA proteins function in concert to protect the genome, a key step being the activation of FANCD2 by mono-ubiquitination...
  10. pmc DNA robustly stimulates FANCD2 monoubiquitylation in the complex with FANCI
    Koichi Sato
    Laboratory of Structural Biology, Graduate School of Advanced Science and Engineering, Waseda University, 2 2 Wakamatsu cho, Shinjuku ku, Tokyo 162 8480, Japan
    Nucleic Acids Res 40:4553-61. 2012
    FANCI and FANCD2 form a complex, and play essential roles in the repair of interstrand DNA crosslinks (ICLs) by the Fanconi anemia (FA) pathway...
  11. pmc FANCI phosphorylation functions as a molecular switch to turn on the Fanconi anemia pathway
    Masamichi Ishiai
    Laboratory of DNA Damage Signaling, Department of Late Effect Studies, Radiation Biology Center, Kyoto University, Yoshidakonoe cho, Sakyo ku, Kyoto 606 8501, Japan
    Nat Struct Mol Biol 15:1138-46. 2008
    ..DNA damage or replication fork stress, the Fanconi anemia pathway is activated, leading to monoubiquitination of FANCD2 and FANCI and their colocalization in foci...
  12. ncbi Fanconi anemia protein complex: mapping protein interactions in the yeast 2- and 3-hybrid systems
    Susan M Gordon
    Program in Genetics and Genomic Biology, Research Institute, Hospital for Sick Children, 555 University Avenue, Toronto, Ontario M5G 1X8, Canada
    Blood 102:136-41. 2003
    ..The FANCA, FANCC, FANCE, FANCF, and FANCG proteins form a nuclear complex required for the monoubiquination of the FANCD2 protein...
  13. pmc Fanconi anemia protein FANCD2 inhibits TRF1 polyADP-ribosylation through tankyrase1-dependent manner
    Alex Lyakhovich
    Department of Genetics and Microbiology, Universitat Autonoma de Barcelona, Edifici C, Bellaterra, Barcelona 08193, Spain
    Genome Integr 2:4. 2011
    ..abstract:..
  14. ncbi FANCI is a second monoubiquitinated member of the Fanconi anemia pathway
    Ashley E Sims
    Department of Biochemistry, New York University School of Medicine, New York, New York 10016, USA
    Nat Struct Mol Biol 14:564-7. 2007
    Activation of the Fanconi anemia (FA) DNA damage-response pathway results in the monoubiquitination of FANCD2, which is regulated by the nuclear FA core ubiquitin ligase complex...
  15. ncbi Regulated interaction of the Fanconi anemia protein, FANCD2, with chromatin
    Rocio Montes De Oca
    Dana Farber Cancer Institute, Department of Radiation Oncology, Harvard Medical School, 44 Binney St, Boston, MA 02115, USA
    Blood 105:1003-9. 2005
    DNA damage activates the monoubiquitination of the Fanconi anemia (FA) protein, FANCD2, resulting in the assembly of FANCD2 nuclear foci...
  16. doi FAN1 acts with FANCI-FANCD2 to promote DNA interstrand cross-link repair
    Ting Liu
    Life Sciences Institute, Zhejiang University, Hangzhou, Zhejiang 310058, China
    Science 329:693-6. 2010
    ..A central event in the FA pathway is mono-ubiquitylation of the FANCI-FANCD2 (ID) protein complex...
  17. pmc Regulation of FANCD2 and FANCI monoubiquitination by their interaction and by DNA
    Simonne Longerich
    Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06520, USA
    Nucleic Acids Res 42:5657-70. 2014
    b>FANCD2 and FANCI function together in the Fanconi anemia network of deoxyribonucleic acid (DNA) crosslink repair...
  18. doi FANCD2 and CtIP cooperate to repair DNA interstrand crosslinks
    Olga Murina
    Institute of Molecular Cancer Research, University of Zurich, Winterthurerstrasse 190, 8057 Zurich, Switzerland
    Cell Rep 7:1030-8. 2014
    ..b>FANCD2 monoubiquitination and CtIP-dependent DNA-end resection represent key events in FA and HR activation, respectively,..
  19. ncbi The Chinese hamster FANCG/XRCC9 mutant NM3 fails to express the monoubiquitinated form of the FANCD2 protein, is hypersensitive to a range of DNA damaging agents and exhibits a normal level of spontaneous sister chromatid exchange
    J B Wilson
    Mammalian DNA Repair Laboratory, School of Biological Sciences, Donnan Laboratories, University of Liverpool, Liverpool, L69 7ZD, UK
    Carcinogenesis 22:1939-46. 2001
    ..containing the FANCA, FANCC, FANCE, FANCF and FANCG proteins is needed for the activation of a sixth FA protein FANCD2. When monoubiquitinated, the FANCD2 protein co-localizes with the breast cancer susceptibility protein BRCA1 in ..
  20. pmc FANCE: the link between Fanconi anaemia complex assembly and activity
    Paul Pace
    MRC Laboratory of Molecular Biology, Hills Road, Cambridge, UK
    EMBO J 21:3414-23. 2002
    ..It activates the downstream protein FANCD2 by monoubiquitylation; this then forges an association with the BRCA1 protein at sites of DNA damage...
  21. pmc ATR couples FANCD2 monoubiquitination to the DNA-damage response
    Paul R Andreassen
    Department of Radiation Oncology, Dana Farber Cancer Institute, Harvard Medical School, Boston, Massachusetts 02115, USA
    Genes Dev 18:1958-63. 2004
    ..The FA pathway regulates the monoubiquitination of FANCD2 and the assembly of damage-associated FANCD2 nuclear foci...
  22. ncbi FANCD2 associated with sporadic breast cancer risk
    E Barroso
    Human Genetics Group, Human Cancer Genetics Programme, Spanish National Cancer Centre CNIO, Madrid, Spain
    Carcinogenesis 27:1930-7. 2006
    ..We studied seven SNPs in FANCA, FANCL and FANCD2 in a total of 897 consecutive and non-related sporadic breast cancer cases and 1033 unaffected controls from the ..
  23. ncbi FANCC, FANCE, and FANCD2 form a ternary complex essential to the integrity of the Fanconi anemia DNA damage response pathway
    Susan M Gordon
    Program in Genetics and Genomic Biology, Research Institute, Hospital for Sick Children, Toronto, Ontario M5G 1X8, Canada
    J Biol Chem 280:36118-25. 2005
    ..of seven of the nine identified FA genes participate in a protein complex required for monoubiquitination of the FANCD2 protein...
  24. ncbi Convergence of the fanconi anemia and ataxia telangiectasia signaling pathways
    Toshiyasu Taniguchi
    Department of Pediatric Oncology, Dana Farber Cancer Institute and Department of Pediatrics, Children s Hospital, Harvard Medical School, Boston, MA 02115, USA
    Cell 109:459-72. 2002
    ..Here, we identify the Fanconi anemia protein, FANCD2, as a link between the FA and ATM damage response pathways. ATM phosphorylates FANCD2 on serine 222 in vitro...
  25. pmc A genetic screen identifies FAN1, a Fanconi anemia-associated nuclease necessary for DNA interstrand crosslink repair
    Agata Smogorzewska
    Howard Hughes Medical Institute, Department of Genetics, Harvard Medical School, and Brigham and Women s Hospital, Boston, MA 02115, USA
    Mol Cell 39:36-47. 2010
    ..complex in a manner dependent on FAN1's ubiquitin-binding domain (UBZ), the ID complex, and monoubiquitination of FANCD2. FAN1 possesses intrinsic 5'-3' exonuclease activity and endonuclease activity that cleaves nicked and branched ..
  26. doi Ku70 corrupts DNA repair in the absence of the Fanconi anemia pathway
    Paul Pace
    MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK
    Science 329:219-23. 2010
    ..Ku70 binds directly to free DNA ends, committing them to NHEJ repair. We show that purified FANCD2, a downstream effector of the FA pathway, might antagonize Ku70 activity by modifying such DNA substrates...
  27. pmc The E3 ubiquitin ligase RAD18 regulates ubiquitylation and chromatin loading of FANCD2 and FANCI
    Stacy A Williams
    Department of Pathology, Yale University School of Medicine, New Haven, CT, USA
    Blood 117:5078-87. 2011
    ..this pathway is the monoubiquitylation and chromatin localization of 2 FA proteins, FA complementation group D2 (FANCD2) and FANCI...
  28. pmc Histone chaperone activity of Fanconi anemia proteins, FANCD2 and FANCI, is required for DNA crosslink repair
    Koichi Sato
    Laboratory of Structural Biology, Graduate School of Advanced Science and Engineering, Waseda University, Tokyo, Japan
    EMBO J 31:3524-36. 2012
    ..A key FA protein, FANCD2, is targeted to chromatin with its partner, FANCI, and plays a critical role in DNA crosslink repair...
  29. ncbi A novel ubiquitin ligase is deficient in Fanconi anemia
    Amom Ruhikanta Meetei
    Laboratory of Genetics, National Institute on Aging, National Institutes of Health, 333 Cassell Drive, TRIAD Center Room 3000, Baltimore, Maryland 21224, USA
    Nat Genet 35:165-70. 2003
    ..1,2). A key step in this pathway is monoubiquitination of FANCD2, resulting in the redistribution of FANCD2 to nuclear foci containing BRCA1 (ref. 3)...
  30. doi Deficiency of FANCD2-associated nuclease KIAA1018/FAN1 sensitizes cells to interstrand crosslinking agents
    Katja Kratz
    Institute of Molecular Cancer Research, University of Zurich, ETH Zurich, Winterthurerstrasse 190, 8057 Zurich, Switzerland
    Cell 142:77-88. 2010
    ..The link of KIAA1018 to the FA pathway is further strengthened by its recruitment to DNA damage through interaction of its UBZ domain with monoubiquitylated FANCD2. We therefore propose to name KIAA1018 FANCD2-associated nuclease, FAN1.
  31. ncbi Interaction of FANCD2 and NBS1 in the DNA damage response
    Koji Nakanishi
    Department of Pediatric Oncology, Dana Farber Cancer Institute, 44 Binney Street, Boston, MA 02115, USA
    Nat Cell Biol 4:913-20. 2002
    ..In wild-type cells, MMC activates the colocalization of the FA subtype D2 protein (FANCD2) and NBS1 protein in subnuclear foci...
  32. pmc Identification of KIAA1018/FAN1, a DNA repair nuclease recruited to DNA damage by monoubiquitinated FANCD2
    Craig MacKay
    MRC Protein Phosphorylation Unit, College of Life Sciences, University of Dundee, Dundee DD1 5EH, Scotland, UK
    Cell 142:65-76. 2010
    ..The FA core complex catalyzes the monoubiquitination of FANCD2, and this event is essential for several steps of ICL repair...
  33. pmc CCAAT/enhancer binding protein delta (C/EBPdelta, CEBPD)-mediated nuclear import of FANCD2 by IPO4 augments cellular response to DNA damage
    Jun Wang
    Laboratory of Cell and Developmental Signaling, Center for Cancer Research, National Cancer Institute at Frederick, Frederick, MD 21702, USA
    Proc Natl Acad Sci U S A 107:16131-6. 2010
    ..studies, we found that C/EBPδ promotes monoubiquitination of the Fanconi anemia complementation group D2 protein (FANCD2), which is necessary for its function in replication-associated DNA repair...
  34. ncbi The nuclear accumulation of the Fanconi anemia protein FANCE depends on FANCC
    France Léveillé
    Department of Clinical Genetics and Human Genetics, VU University Medical Center, Van der Boechorststraat 7, 1081 BT Amsterdam, The Netherlands
    DNA Repair (Amst) 5:556-65. 2006
    ..component of the nuclear FA core complex, which is required for monoubiquitination of the downstream target FANCD2, an important step in the FA pathway of DNA cross-link repair...
  35. ncbi The Fanconi anemia pathway is required for the DNA replication stress response and for the regulation of common fragile site stability
    Niall G Howlett
    Department of Human Genetics, University of Michigan, Ann Arbor, MI 48109, USA
    Hum Mol Genet 14:693-701. 2005
    ..damaging agents, and during S-phase of the cell cycle, the FA pathway is activated via the mono-ubiquitination of FANCD2 (FANCD2-Ub), signaling its translocation to discrete nuclear foci, where it co-localizes with the central DNA ..
  36. pmc Alcohol metabolism in human cells causes DNA damage and activates the Fanconi anemia-breast cancer susceptibility (FA-BRCA) DNA damage response network
    Jessy Abraham
    Section on Molecular Neurobiology, Laboratory of Neurogenetics, National Institute on Alcohol Abuse and Alcoholism, NIH, Rockville, Maryland, USA
    Alcohol Clin Exp Res 35:2113-20. 2011
    ..We recently reported that exposure of human cells in vitro to acetaldehyde resulted in the activation of the Fanconi anemia-breast cancer susceptibility (FA-BRCA) DNA damage response network...
  37. pmc Hypomorphic mutations in the gene encoding a key Fanconi anemia protein, FANCD2, sustain a significant group of FA-D2 patients with severe phenotype
    Reinhard Kalb
    Department of Human Genetics, University of Wurzburg, Wurzburg, Germany
    Am J Hum Genet 80:895-910. 2007
    b>FANCD2 is an evolutionarily conserved Fanconi anemia (FA) gene that plays a key role in DNA double-strand-type damage responses...
  38. pmc A human ortholog of archaeal DNA repair protein Hef is defective in Fanconi anemia complementation group M
    Amom Ruhikanta Meetei
    Division of Experimental Hematology, Cincinnati Children s Hospital Research Foundation and University of Cincinnati College of Medicine, 3333 Burnet Avenue, Cincinnati, Ohio 45229, USA
    Nat Genet 37:958-63. 2005
    ..Each protein in this complex is essential for monoubiquitination of FANCD2, a key reaction in the Fanconi anemia DNA damage-response pathway...
  39. pmc A role for monoubiquitinated FANCD2 at telomeres in ALT cells
    Qiang Fan
    Division of Experimental Hematology and Cancer Biology, Cincinnati Children s Research Foundation, Department of Pediatrics, University of Cincinnati College of Medicine, Cincinnati, OH 45229, USA
    Nucleic Acids Res 37:1740-54. 2009
    ..Because of these similarities, we have investigated whether there is a relationship between the FA protein, FANCD2 and telomeres...
  40. ncbi X-linked inheritance of Fanconi anemia complementation group B
    Amom Ruhikanta Meetei
    Laboratory of Genetics, National Institute on Aging, National Institutes of Health, 333 Cassell Drive, TRIAD Center Room 3000, Baltimore, Maryland 21224, USA
    Nat Genet 36:1219-24. 2004
    ..group B is an essential component of the nuclear protein 'core complex' responsible for monoubiquitination of FANCD2, a key event in the DNA-damage response pathway associated with Fanconi anemia and BRCA...
  41. pmc Histone H2AX and Fanconi anemia FANCD2 function in the same pathway to maintain chromosome stability
    Massimo Bogliolo
    Group of Mutagenesis, Department of Genetics and Microbiology, Universitat Autonoma de Barcelona, Bellaterra, Barcelona, Spain
    EMBO J 26:1340-51. 2007
    ..The central FA protein FANCD2 is known to relocate to chromatin upon DNA damage in a poorly understood process...
  42. pmc Functional interaction of monoubiquitinated FANCD2 and BRCA2/FANCD1 in chromatin
    XiaoZhe Wang
    Department of Pediatric Oncology, Dana Farber Cancer Institute, Harvard Medical School, Boston, Massachusetts 02115, USA
    Mol Cell Biol 24:5850-62. 2004
    ..Here we show that monoubiquitination of FANCD2, which is activated by DNA damage, is required for targeting of FANCD2 to chromatin, where it interacts with BRCA2...
  43. pmc HPV-16 E7 reveals a link between DNA replication stress, fanconi anemia D2 protein, and alternative lengthening of telomere-associated promyelocytic leukemia bodies
    Nicole Spardy
    Biochemistry and Molecular Genetics Graduate Program, and Department of Pathology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15213, USA
    Cancer Res 68:9954-63. 2008
    ..proteins that are involved in the response to DNA replication stress, most notably the Fanconi anemia D2 protein (FANCD2) as well as BRCA2 and MUS81...
  44. ncbi The WD40 repeats of FANCL are required for Fanconi anemia core complex assembly
    Allan M Gurtan
    Biological and Biomedical Sciences Program, Dana Farber Cancer Institute, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Biol Chem 281:10896-905. 2006
    ..form a multisubunit nuclear complex (FA core complex) required for monoubiquitination of a downstream FA protein, FANCD2. FANCL, which possesses three WD40 repeats and a plant homeodomain (PHD), is the putative E3 ubiquitin ligase ..
  45. ncbi Direct interaction of FANCD2 with BRCA2 in DNA damage response pathways
    Shobbir Hussain
    Division of Genetics and Development, Guy s, King s and St Thomas s School of Medicine, King s College London, UK
    Hum Mol Genet 13:1241-8. 2004
    ..proteins encoded by the known FA genes form a nuclear complex which is required for the monoubiquitination of the FANCD2 protein...
  46. pmc Regulation of the Fanconi anemia pathway by a CUE ubiquitin-binding domain in the FANCD2 protein
    Meghan A Rego
    Department of Cell and Molecular Biology, University of Rhode Island, Kingston, RI 02881, USA
    Blood 120:2109-17. 2012
    ..A key step in FA-BRCA pathway activation is the covalent attachment of monoubiquitin to FANCD2 and FANCI...
  47. ncbi Direct DNA binding activity of the Fanconi anemia D2 protein
    Woo Hyun Park
    Department of Pathology, Brigham and Women s Hospital and Harvard Medical School, Boston, Massachusetts 02115, USA
    J Biol Chem 280:23593-8. 2005
    ..In these experiments we purified full-length Fanconi anemia protein D2 (FANCD2), and we found that FANCD2 bound to DNA with specificity for certain structures: double strand DNA ends and ..
  48. pmc Functional interaction between the Fanconi Anemia D2 protein and proliferating cell nuclear antigen (PCNA) via a conserved putative PCNA interaction motif
    Niall G Howlett
    Department of Cell and Molecular Biology, University of Rhode Island, Kingston, Rhode Island 02881, USA
    J Biol Chem 284:28935-42. 2009
    ..Activation of this pathway occurs via the mono-ubiquitination of the FANCD2 protein, targeting it to nuclear foci where it co-localizes with FANCD1/BRCA2, RAD51, and PCNA...
  49. ncbi BRCA1-independent ubiquitination of FANCD2
    Cassandra J Vandenberg
    Protein and Nucleic Acid Chemistry Division, MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, United Kingdom
    Mol Cell 12:247-54. 2003
    Monoubiquitination of the FANCD2 protein is a key step in the Fanconi anemia (FA) tumor suppressor pathway, coinciding with this molecule's accumulation at sites of genome damage...
  50. pmc Convergence of Rad6/Rad18 and Fanconi anemia tumor suppressor pathways upon DNA damage
    Hwan Ki Park
    The Hormel Institute, University of Minnesota, Austin, Minnesota, United States of America
    PLoS ONE 5:e13313. 2010
    ..We previously reported that FANCD2 monoubiquitination, a hallmark of the FA pathway activation, can be regulated by HHR6...
  51. pmc Loss of expression of FANCD2 protein in sporadic and hereditary breast cancer
    Petra van der Groep
    Department of Pathology, University Medical Center, Utrecht, The Netherlands
    Breast Cancer Res Treat 107:41-7. 2008
    ..Activation of one of the FA proteins (FANCD2) by mono-ubiquitination is an essential step in DNA damage response...
  52. doi A FANCD2 domain activates Tip60-dependent apoptosis
    James Hejna
    Department of Molecular and Medical Genetics, Oregon Health and Science University, 3181 SW Sam Jackson Park Road, Portland, OR 97239 3098, USA
    Cell Biol Int 34:893-9. 2010
    The FA (Fanconi anaemia) FANCD2 protein is pivotal in the cellular response to DNA interstrand cross-links. Establishing cells expressing exogenous FANCD2 has proven to be difficult compared with other DNA repair genes...
  53. ncbi Positional cloning of a novel Fanconi anemia gene, FANCD2
    C Timmers
    Department of Molecular and Medical Genetics, Oregon Health Sciences University, Portland, OR 97201, USA
    Mol Cell 7:241-8. 2001
    ..Complementation group D is heterogeneous, consisting of two distinct genes, FANCD1 and FANCD2. Here we report the positional cloning of FANCD2...
  54. doi Fanconi anemia complementation group FANCD2 protein serine 331 phosphorylation is important for fanconi anemia pathway function and BRCA2 interaction
    Gang Zhi
    Department of Microbiology, University of Virginia Health System, Charlottesville, Virginia, USA
    Cancer Res 69:8775-83. 2009
    ..failure and cancer susceptibility syndrome with at least 13 complementation groups (FANCA, FANCB, FANCC, FANCD1, FANCD2, FANCE, FANCF, FANCG, FANCI, FANCJ, FANCL, FANCM, and FANCN)...
  55. pmc Localization of the Fanconi anemia complementation group D gene to a 200-kb region on chromosome 3p25.3
    J A Hejna
    Department of Molecular and Medical Genetics, Oregon Health Sciences University, Portland, OR 97201, USA
    Am J Hum Genet 66:1540-51. 2000
    ..The FA complementation group D gene (FANCD) has previously been localized to chromosome 3p22-26, by use of microcell-mediated chromosome transfer...
  56. ncbi Xanthine oxidase and xanthine dehydrogenase from an estivating land snail
    M Hermes Lima
    Institute of Biochemistry, Carleton University, Ottawa, Ontario, Canada
    Z Naturforsch C 50:685-94. 1995
    ..that the formation of an enzyme-methylene blue complex induces protection against heat and oxidative damage at the FAD-active site...
  57. ncbi Impaired non-esterified fatty acid suppression is associated with endothelial dysfunction in insulin resistant subjects
    B M Balletshofer
    Department of Endocrinology and Metabolism, , Germany
    Horm Metab Res 33:428-31. 2001
    ..Endothelial function was measured as flow-associated vasodilation (FAD%) of the brachial artery...
  58. pmc Rad18 E3 ubiquitin ligase activity mediates Fanconi anemia pathway activation and cell survival following DNA Topoisomerase 1 inhibition
    Komaraiah Palle
    University of North Carolina, Chapel Hill, NC USA
    Cell Cycle 10:1625-38. 2011
    ..We show here that the FA core complex protein FANCA and monoubiquitinated FANCD2 (an effector of the FA pathway) are rapidly mobilized to chromatin in response to CPT treatment in several human ..
  59. ncbi Crystal structure of Escherichia coli thioredoxin reductase refined at 2 A resolution. Implications for a large conformational change during catalysis
    G Waksman
    Laboratory of Molecular Biophysics, Rockefeller University, New York, NY 10021
    J Mol Biol 236:800-16. 1994
    ..adenine dinucleotide phosphate (NADPH) to a disulfide bond in the enzyme, via a flavin adenine dinucleotide (FAD)...
  60. ncbi Blue light mediated photoreduction of the flavoprotein NADPH-cytochrome P450 reductase. A Förster-type energy transfer
    D Muller-Enoch
    Abteilung Physiologische Chemie, Universitat Ulm, Germany
    Z Naturforsch C 52:605-14. 1997
    The absorption spectra and the corresponding molar absorption coefficients of the fluorophores umbelliferone, FAD and FMN and of the FAD and FMN containing flavoprotein NADPH-cytochrome P450 reductase of different oxydation-reduction ..
  61. ncbi Regulation of nitrogen fixation in Klebsiella pneumoniae and Azotobacter vinelandii: NifL, transducing two environmental signals to the nif transcriptional activator NifA
    Ruth A Schmitz
    Institut fur Mikrobiologie und Genetik, Gottingen, Germany
    J Mol Microbiol Biotechnol 4:235-42. 2002
    ..of the negative regulator NifL appears to lie in the C-terminal domain, whereas the N-terminal domain binds FAD as a redox-sensitive cofactor, which is required for signal transduction of the internal oxygen status...
  62. ncbi Genes and mechanisms involved in beta-amyloid generation and Alzheimer's disease
    H Steiner
    Adolf-Butenandt-Institute, Ludwig-Maximilians-University, Dept. of Biochemistry, Munich, Germany
    Eur Arch Psychiatry Clin Neurosci 249:266-70. 1999
    ..In some very rare cases of familial AD (FAD), mutations have been identified within the betaAPP gene...
  63. ncbi FANCD2 Western blot as a diagnostic tool for Brazilian patients with Fanconi anemia
    D V Pilonetto
    Laboratorio de Imunogenetica, Hospital de Clinicas, Universidade Federal do Parana, Curitiba, PR, Brasil
    Braz J Med Biol Res 42:237-43. 2009
    ..in molecular pathogenesis of this disease led researchers to develop a diagnostic test based on Western blot for FANCD2. The objective of the present study was to determine the efficacy of this method for the diagnosis of 84 Brazilian ..
  64. ncbi Synergistic interactions of multiple mutations on catalysis during the hydroxylation reaction of p-hydroxybenzoate hydroxylase: studies of the Lys297Met, Asn300Asp, and Tyr385Phe mutants reconstituted with 8-Cl-flavin
    M Ortiz Maldonado
    Department of Biological Chemistry, University of Michigan, Ann Arbor, Michigan 48109 0606
    Biochemistry 40:8705-16. 2001
    ..We have used wild-type (WT) PHBH and several mutant forms, all with normal FAD and with 8-Cl-FAD substituted for FAD, to assess the relative contributions of the two effects...
  65. pmc Inhibitory actions of diphenyleneiodonium on endothelium-dependent vasodilatations in vitro and in vivo
    Y X Wang
    Department of Pharmacology and Therapeutics, Faculty of Medicine, University of British Columbia, Vancouver, Canada
    Br J Pharmacol 110:1232-8. 1993
    ..effect of DPI (3 x 10(-7) M) on ACh-induced relaxation was prevented by pretreatment with NADPH (5 x 10(-3) M) and FAD (5 x 10(-4) M) but not L-arginine (L-Arg, 2 x 10(-3) M)...
  66. ncbi Global effects of the energetics of coenzyme binding: NADPH controls the protein interaction properties of human cytochrome P450 reductase
    Alex Grunau
    Department of Biochemistry, University of Leicester, UK
    Biochemistry 45:1421-34. 2006
    The thermodynamics of coenzyme binding to human cytochrome P450 reductase (CPR) and its isolated FAD-binding domain have been studied by isothermal titration calorimetry...
  67. doi Facile oxidation of leucomethylene blue and dihydroflavins by artemisinins: relationship with flavoenzyme function and antimalarial mechanism of action
    Richard K Haynes
    Department of Chemistry, Institute of Molecular Technology for Drug Discovery and Synthesis, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, P R China
    ChemMedChem 5:1282-99. 2010
    ..Dihydroflavins generated from riboflavin (RF) and FAD by pretreatment with sodium dithionite are rapidly oxidised by artemisinin to the parent flavins...
  68. pmc Genomic instability in mice is greater in Fanconi anemia caused by deficiency of Fancd2 than Fancg
    Ramune Reliene
    Cancer Research Center, Department of Environmental Health Sciences, University at Albany, State University of New York, Albany, New York, USA
    Cancer Res 70:9703-10. 2010
    ..FANCG is a part of the FA core complex that is responsible for monoubiquitination of FANCD2 and FANCI...
  69. pmc Electron transfer flavoprotein domain II orientation monitored using double electron-electron resonance between an enzymatically reduced, native FAD cofactor, and spin labels
    Michael A Swanson
    Department of Chemistry and Biochemistry, University of Denver, Denver, Colorado 80208, USA
    Protein Sci 20:610-20. 2011
    ..structure of human ETF bound to medium chain acyl-CoA dehydrogenase indicates that the flavin adenine dinucleotide (FAD) domain (αII) is mobile, which permits more rapid electron transfer with donors and acceptors by providing closer ..
  70. ncbi Gene-specific selection against experimental fanconi anemia gene inactivation in human cancer
    Eike Gallmeier
    The Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins University, Baltimore, Maryland, USA
    Cancer Biol Ther 6:654-60. 2007
    ..the sequential homozygous deletion was achieved only for FANCC and FANCG, acting upstream, but not for BRCA2 or FANCD2, acting downstream in the FA pathway...
  71. ncbi A novel role for Fanconi anemia (FA) pathway effector protein FANCD2 in cell cycle progression of untransformed primary human cells
    Ihn Young Song
    Department of Genetics and Genomics, Boston University School of Medicine, Boston, MA, USA
    Cell Cycle 9:2375-88. 2010
    ..The Fanconia Anemia D2 (FANCD2) protein is implicated in repair of various forms of DNA damage including ICLs...
  72. ncbi Oxygen reactions in p-hydroxybenzoate hydroxylase utilize the H-bond network during catalysis
    Mariliz Ortiz-Maldonado
    Department of Biological Chemistry, University of Michigan, Ann Arbor, Michigan 48109 0606, USA
    Biochemistry 43:15246-57. 2004
    ..a flavoprotein monooxygenase that catalyses a reaction in two parts: reduction of the flavin adenine dinucleotide (FAD) in the enzyme by reduced nicotinamide adenine dinucleotide phosphate (NADPH) in response to binding p-..
  73. pmc Monoketone analogs of curcumin, a new class of Fanconi anemia pathway inhibitors
    Igor Landais
    Department of Biochemistry and Molecular Biology, Oregon Health and Science University, Portland, USA
    Mol Cancer 8:133. 2009
    ..Thus, inhibition of FANCD2 monoubiquitylation (FANCD2-Ub), a key step in the FA pathway, might target tumor cells defective in ATM through ..
  74. ncbi The Fanconi anemia protein, FANCE, promotes the nuclear accumulation of FANCC
    Toshiyasu Taniguchi
    Department of Pediatric Oncology, Dana Farber Cancer Institute, and the Department of Pediatrics, Children s Hospital, Harvard Medical School, Boston, MA 02115, USA
    Blood 100:2457-62. 2002
    ..The 6 known Fanconi anemia gene products (FANCA, FANCC, FANCD2, FANCE, FANCF, and FANCG proteins) interact in a common pathway...
  75. pmc UBE2W interacts with FANCL and regulates the monoubiquitination of Fanconi anemia protein FANCD2
    Yingying Zhang
    Laboratory of Protein Engineering, Institute of Biotechnology, Beijing, China
    Mol Cells 31:113-22. 2011
    ..disease mostly caused by improper regulation of the monoubiquitination of Fanconi anemia complementation group D2 (FANCD2)...
  76. ncbi [Doppler evaluation of blood flow of the lower limb]
    X Chanudet
    , ,
    Arch Mal Coeur Vaiss 83:1169-74. 1990
    ..BFLL estimation depends on common femoral artery diameter (FAD) echographic measurement at the thigh root and on velocity time interval (VTI) calculation with pulsed wave doppler ..
  77. ncbi Characterization of the flavoprotein moieties of NADPH-sulfite reductase from Salmonella typhimurium and Escherichia coli. Physicochemical and catalytic properties, amino acid sequence deduced from DNA sequence of cysJ, and comparison with NADPH-cytochrom
    J Ostrowski
    Howard Hughes Medical Institute Laboratory, Duke University Medical Center, Durham, North Carolina 27710
    J Biol Chem 264:15796-808. 1989
    ..and deduced amino acid sequences indicate that SiR-FP is an octamer of identical 66-kDa peptides and contains 4 FAD and 4 FMN per octamer...
  78. ncbi Heterozygosity for p53 (Trp53+/-) accelerates epithelial tumor formation in fanconi anemia complementation group D2 (Fancd2) knockout mice
    Scott Houghtaling
    Department of Molecular and Medical Genetics, Oregon Health and Science University, Portland, OR 97239, USA
    Cancer Res 65:85-91. 2005
    ..We have previously reported an increased incidence of epithelial tumors in Fancd2 knockout mice...
  79. ncbi X-ray structure of the ferredoxin:NADP+ reductase from the cyanobacterium Anabaena PCC 7119 at 1.8 A resolution, and crystallographic studies of NADP+ binding at 2.25 A resolution
    L Serre
    IBS LCCP, Grenoble, France
    J Mol Biol 263:20-39. 1996
    ..172. The model includes 303 residues, the flavin adenine dinucleotide cofactor (FAD), one sulfate ion located at the putative NADP+ binding site and 328 water molecule sites...
  80. ncbi Small cerebral vessel disease in familial amyloid and non-amyloid angiopathies: FAD-PS-1 (P117L) mutation and CADASIL. Immunohistochemical and ultrastructural studies
    Grazyna M Szpak
    Department of Neuropathology, Institute of Psychiatry and Neurology, Sobieskiego 9, 02 957 Warsaw, Poland
    Folia Neuropathol 45:192-204. 2007
    ..Two cases, previously diagnosed as familial Alzheimer's disease (FAD) with cerebral amyloid angiopathy (CAA), were confirmed by genetic and neuropathological studies, and one case of ..
  81. ncbi Histidine 61: an important heme ligand in the soluble fumarate reductase from Shewanella frigidimarina
    Emma L Rothery
    School of Chemistry, University of Edinburgh, UK
    Biochemistry 42:13160-9. 2003
    ..Biol. 6, 1108-1112] shows the presence of four, bis-His-ligated, c-type hemes and one flavin adenine dinucleotide, FAD. The heme groups provide a "molecular wire" for the delivery of electrons to the FAD...
  82. ncbi Steady state and picosecond time-resolved fluorescence studies on native, desulpho and deflavo xanthine oxidase
    A K Sau
    Department of Chemical Sciences, Tata Institute of Fundamental Research, Homi Bhabha Road, Colaba, 400 005, Mumbai, India
    Biochim Biophys Acta 1481:273-82. 2000
    ..replacement of the molybdenum double bonded sulphur by oxygen and the removal of the flavin adenine dinucleotide (FAD)...
  83. ncbi Efficacy and safety of selamectin against fleas and heartworms in dogs and cats presented as veterinary patients in North America
    M G Boy
    Animal Health Clinical Affairs, Central Research Division, Pfizer Inc, Groton, CT 06340, USA
    Vet Parasitol 91:233-50. 2000
    ..made on the beneficial effect of selamectin treatment on dogs and cats showing signs of flea allergy dermatitis (FAD)...
  84. ncbi Purification of electron-transferring flavoprotein from Megasphaera elsdenii and binding of additional FAD with an unusual absorption spectrum
    Kyosuke Sato
    Department of Molecular Physiology, Graduate School of Medical Sciences, Kumamoto University, 1 1 1 Honjo, Kumamoto, Kumamoto, 860 8556
    J Biochem 134:719-29. 2003
    ..The purified ETF contained one mol FAD/mol ETF as the sole non-protein component and bound almost one mol of additional FAD...
  85. ncbi Functional restoration. Returning patients with chronic low back pain to work--revolution or fad?
    R W Teasell
    Department of Physical Medicine and Rehabilitation, University Hospital, London, Ontario, Canada
    Spine (Phila Pa 1976) 21:844-7. 1996
    ..A review was performed to assess the basis for functional restoration programs in returning patients with chronic low back pain to work...
  86. ncbi Purification and characterisation of the NADH:acceptor reductase component of xylene monooxygenase encoded by the TOL plasmid pWW0 of Pseudomonas putida mt-2
    J P Shaw
    Department of Medical Biochemistry, University Medical Center, Geneve, Switzerland
    Eur J Biochem 209:51-61. 1992
    ..The enzyme was found to contain 1 mol/mol of tightly but not covalently bound FAD, as well as 2 mol/mol of non-haem iron and 2 mol/mol of acid-labile sulfide, suggesting the presence of two redox ..
  87. pmc Cloning and characterization of FAD1, the structural gene for flavin adenine dinucleotide synthetase of Saccharomyces cerevisiae
    M Wu
    Department of Biological Sciences, Columbia University, New York, New York 10027
    Mol Cell Biol 15:264-71. 1995
    ..Mutants in this group display a reduced level of flavin adenine dinucleotide (FAD) and an increased level of flavin mononucleotide (FMN) in mitochondria...
  88. ncbi Spectroscopic properties of Escherichia coli UDP-N-acetylenolpyruvylglucosamine reductase
    M J Axley
    Enzymology Laboratory, Bristol Myers Squibb Pharmaceutical Research Institute, Princeton, New Jersey 08543 4000, USA
    Biochemistry 36:812-22. 1997
    ..and UV-visible spectroscopy to establish the spectral properties of its tightly bound flavin adenine dinucleotide (FAD) cofactor...
  89. ncbi Cytochrome b5 reductase: the roles of the recessive congenital methemoglobinemia mutants P144L, L148P, and R159*
    C Ainsley Davis
    Department of Biochemistry and Molecular Biology, College of Medicine, University of South Florida, Tampa, FL 33612, USA
    Arch Biochem Biophys 431:233-44. 2004
    ..of the primary sequence composed of residues G143 to V171 which serves as a "hinge" or "linker" region between the FAD- and NADH-binding lobes of the protein...
  90. ncbi Low mitochondrial glycerophosphate dehydrogenase activity in lymphocytes of women with gestational diabetes
    J Vidal
    Endocrinology Unit, Hospital Clinic, Barcelona, Spain
    Horm Metab Res 29:60-2. 1997
    An abnormally low activity of mitochondrial FAD-linked glycerophosphate dehydrogenase (m-GDH), relative to the paired measurement of glutamate dehydrogenase, was found in CD3+ lymphocytes from 4 out of 14 mothers with gestational ..
  91. ncbi A critical review of cellobiose dehydrogenases
    G Henriksson
    Department of Pulp and Paper Chemistry and Technology, Royal Institute of Technology, 100 44, Stockholm, Sweden
    J Biotechnol 78:93-113. 2000
    ..CDH that adsorbs strongly and specifically to cellulose carries two prosthetic groups; namely, an FAD and a heme in two different domains that can be separated after limited proteolysis...
  92. ncbi Structure of alpha-glycerophosphate oxidase from Streptococcus sp.: a template for the mitochondrial alpha-glycerophosphate dehydrogenase
    Timothy Colussi
    Center for Structural Biology, Wake Forest University School of Medicine, Winston Salem, North Carolina 27157, USA
    Biochemistry 47:965-77. 2008
    The FAD-dependent alpha-glycerophosphate oxidase (GlpO) from Enterococcus casseliflavus and Streptococcus sp...
  93. doi A fluorescence perspective on the differential interaction of riboflavin and flavin adenine dinucleotide with cucurbit[7]uril
    Sharmistha Dutta Choudhury
    Radiation and Photochemistry Division, Bhabha Atomic Research Centre, Trombay, Mumbai 400 085, India
    J Phys Chem B 114:10717-27. 2010
    ..the macrocyclic host, cucurbit[7]uril (CB7), with riboflavin (RF) and its derivative, flavin adenine dinucleotide (FAD), has been investigated using absorption and steady-state and time-resolved fluorescence measurements...
  94. doi [Determinants of condom use and heterosexual multiple sexual partnership in French Antilles and French Guiana]
    A Cobat
    Observatoire régional de santé d Ile de France, Paris, France
    Rev Epidemiol Sante Publique 56:143-57. 2008
    ..The French-American departments (Guadeloupe, Martinique, Guiana), FAD, are located in the heart of this region...
  95. ncbi The flavoprotein component of the Escherichia coli sulfite reductase: expression, purification, and spectral and catalytic properties of a monomeric form containing both the flavin adenine dinucleotide and the flavin mononucleotide cofactors
    M Zeghouf
    Laboratoire de Chimie et Biochimie des Centres Redox Biologiques, CEA Grenoble, DBMS CBCRB CNRS Université Joseph Fourier, France
    Biochemistry 37:6114-23. 1998
    The flavoprotein component (SiR-FP) of the sulfite reductase from Escherichia coli is an octamer containing one FAD and one FMN per polypeptide chain. SiR-FP60, a SiR-FP fragment starting with alanine-52, was overexpressed in E...
  96. pmc The iron-sulfur cluster of electron transfer flavoprotein-ubiquinone oxidoreductase is the electron acceptor for electron transfer flavoprotein
    Michael A Swanson
    Department of Chemistry and Biochemistry, University of Denver, Denver, Colorado 80208, USA
    Biochemistry 47:8894-901. 2008
    ..It contains one [4Fe-4S] (2+,1+) and one FAD, which are diamagnetic in the isolated oxidized enzyme and can be reduced to paramagnetic forms by enzymatic donors ..
  97. ncbi Mechanism of coenzyme binding to human methionine synthase reductase revealed through the crystal structure of the FNR-like module and isothermal titration calorimetry
    Kirsten R Wolthers
    Faculty of Life Sciences, University of Manchester, Manchester Interdisciplinary Biocentre, 131 Princess Street, Manchester M1 7DN, United Kingdom
    Biochemistry 46:11833-44. 2007
    ..MSR contains one FAD and one FMN cofactor per polypeptide and functions in the sequential transfer of reducing equivalents from NADPH to ..
  98. ncbi Relaxation kinetics of cytochrome P450 reductase: internal electron transfer is limited by conformational change and regulated by coenzyme binding
    Aldo Gutierrez
    Biological NMR Centre, University of Leicester, Medical Sciences Building, P O Box 138, University Road, Leicester LE1 9HN, UK
    Biochemistry 41:4626-37. 2002
    ..electron/hydride transfer and is consistent with its origin in local conformational change in the vicinity of the FAD isoalloxazine ring...
  99. ncbi Four crystal structures of the 60 kDa flavoprotein monomer of the sulfite reductase indicate a disordered flavodoxin-like module
    A Gruez
    Laboratoire de Cristallographie et Cristallogenèse des Protéines Institut de Biologie Structurale J P Ebel, CEA CNRS, Grenoble, France
    J Mol Biol 299:199-212. 2000
    ..Each beta-subunit contains a Fe4S4 cluster and a siroheme, and each alpha-subunit binds one FAD and one FMN as prosthetic groups...
  100. ncbi Evaluation of a 15-day screening assay using intact male rats for identifying steroid biosynthesis inhibitors and thyroid modulators
    John C O'Connor
    DuPont Haskell Laboratory for Health and Environmental Sciences, P O Box 50, Newark, Delaware 19714, USA
    Toxicol Sci 69:79-91. 2002
    ..The test compounds included the aromatase inhibitor fadrozole (FAD), the testosterone biosynthesis inhibitor ketoconazole (KETO), and the thyroid modulators phenobarbital (PB) and ..
  101. ncbi The Na+-translocating NADH:ubiquinone oxidoreductase from Vibrio alginolyticus--redox states of the FAD prosthetic group and mechanism of Ag+ inhibition
    J Steuber
    ETH Zentrum, Zurich, Switzerland
    Eur J Biochem 249:770-6. 1997
    The FAD prosthetic group of the Na+-motive NADH:ubiquinone oxidoreductase (Na+-NQR) from Vibrio alginolyticus was investigated by ultraviolet-visible and fluorescence spectroscopy...

Research Grants66

  1. Pharmacogenomics of Human P450 Oxidoreductase
    Walter L Miller; Fiscal Year: 2012
    ..POR is a 680 amino-acid, 78 kDa protein that contains two flavins, a flavin adenine dinucleotide moiety (FAD), and a flavin mononucleotide (FMN). There is substantial genetic variation in the hepatic P450 enzymes...
  2. Non-invasive Assessment of Tissue Engineered Human Oral Mucosa
    Mary Ann Mycek; Fiscal Year: 2012
    ..of the cellular component of the EVPOME via the redox state of endogenous fluorophores flavin adenine dinucleotide (FAD) and nicotinamide adenine dinucleotide (NADH)...
  3. Detection strategies:Aptamer-based target recognition to turn-on GOx signaling
    MEHNAAZ FATIMA ALI; Fiscal Year: 2013
    ..The redox properties of GOx are dependent on the presence of its co-factor flavin adenine dinucleotide (FAD). The removal of FAD reversibly renders the enzyme inactive without permanently denaturing the enzyme structure...
  4. Mechanism of cryptochrome-mediated photo transduction
    Todd C Holmes; Fiscal Year: 2013
    ..sensitivity is mediated through light-activated changes in the redox state of the flavin adenine dinucleotide (FAD) chromophore bound to dCry which then couples to a redox sensor in cytoplasmic potassium channel subunits and ..
  5. Mitoenergetic Failure in Brain Aging
    Gregory J Brewer; Fiscal Year: 2013
    ..higher levels of ROS with lower glutathione antioxidant and maintain a more oxidized redox potential [ NAD(P)H / FAD ], all of which contribute to increased susceptibility to toxic stressors such as glutamate and beta-amyloid...
  6. Employing Familial AD Induced Pluripotent Stem Cells to Study Neurodegeneration
    Suman Jayadev; Fiscal Year: 2013
    ..2 (PSEN2) deficiency is associated with an exaggerated pro-inflammatory response in microglia and that the fAD associated PSEN2 N141I mutation leads to decreased gamma- secretase activity in microglia...
  7. Role of cofilin pathology in mouse models of cognitive impairment
    James R Bamburg; Fiscal Year: 2013
    ..rods that initiate and exacerbate synaptic dysfunction typical of both sporadic (SAD) and familial AD (FAD)...
  8. Presenilin 2 and Neuroinflammation
    Suman Jayadev; Fiscal Year: 2013
    ..Familial Alzheimer disease (fAD) is associated with mutations in the genes encoding amyloid precursor protein (APP), presenilin 1 (PS1) and ..
  9. Cations and ROS in Modulating Mitochondrial Function in Normal and Ischemic Heart
    Ranjan K Dash; Fiscal Year: 2013
    ..bioenergetics and electrophysiology (changes in trans-matrix cation concentrations and fluxes, NADH and FAD redox states, membrane potential, respiration, and ROS production with normal and abnormal perturbations in extra-..
  10. An iPS Disease-in-a-Dish Model of Familial Alzheimers
    Terrence Town; Fiscal Year: 2012
    ..In this regard, transgenic mice harboring mutations in one or more genes that cause early-onset familial AD (fAD) have been enormously helpful, both in terms of interrogating potential therapeutic targets and also for ..
  11. Development of a HTS assay for modulators of presenilin 1 conformation
    Oksana Berezovska; Fiscal Year: 2010
    ..over 150 known mutations in PS1, scattered throughout the molecule, all lead to autosomal dominant familial AD (FAD) and enhance A[unreadable] 42 generation...
  12. Central and endocrine stress pathway contributions to Alzheimers Disease
    Nicholas J Justice; Fiscal Year: 2013
    ..First, I plan to characterize aging mice carrying Familial Alzheimer's Disease (FAD) mutations that acquire AD pathologies, for changes in anxiety related behavior, HPA axis function, and molecular ..
  13. Imaging the neuronal and metabolic basis of resting state connectivity mapping
    Elizabeth M C Hillman; Fiscal Year: 2013
    ..We will also map fluctuations in flavoprotein (FAD) autofluorescence with spontaneous hemodynamics as a measure of local oxidative metabolism, both complemented with ..
  14. PS1 regulates processing and signaling of ephrinB/EphB
    Nikolaos K Robakis; Fiscal Year: 2013
    ..activities of ephrinBLs depend on EphB receptors (EphBRs) and presenilini (PS1), a protein involved in familial AD (FAD). We also observed that PS1 mediates the neuroprotective effects of brain-derived neurotrophic factor (BDNF)...
  15. Structure/Function of Complex II Oxidoreductase
    Gary Cecchini; Fiscal Year: 2012
    ..These include a covalently-bound FAD cofactor, three distinct iron- sulfur clusters, a membrane-bound quinone, and a b heme cofactor...
  16. Insulin/Insulin receptor modulate APP phosphorylation and dementia
    LUCIANO D'ADAMIO; Fiscal Year: 2013
    ..two subunits of an enzyme that regulates APP processing, PSEN1 and PSEN2, cause Familial Alzheimer's disease (FAD). New evidence from our laboratory further stress this link between APP processing and dementia...
  17. Knockin Mouse Models of Alzheimers Disease
    Hui Zheng; Fiscal Year: 2013
    ..Mutations in APP and presenilin (PSEN) genes lead to early onset of familial AD (FAD), establishing their critical roles in AD pathogenesis...
  18. Targeted Mouse Models of Alzheimer Disease
    Ken Ichiro Fukuchi; Fiscal Year: 2001
    ..Approximately 10% of AD cases are classified as early onset familial AD (FAD) and show autosomal dominant inheritance...
  19. Novel Diagnostics to Detect Lung Injury
    Elizabeth R Jacobs; Fiscal Year: 2013
    ..metabolic coenzymes Nicotinamide Adenine Dinucleotide (reduced form is NADH) and Flavine Adenine Dinucleotide (FAD) are the primary electron carriers in oxidative phosphorylation...
  20. Twenty-second Annual Fanconi Anemia Research Fund Scientific Symposium
    GROVER CARLTON BAGBY; Fiscal Year: 2010
    ..pathway," eight of the eleven known Fanconi anemia proteins bind together in a complex and monoubiquitinate FANCD2, one of the proteins not in the "core" complex...
  21. 24th Annual Fanconi Anemia Research Fund Scientific Symposium
    GROVER CARLTON BAGBY; Fiscal Year: 2012
    ..of the fifteen known Fanconi anemia proteins bind together in a complex and facilitate the monoubiquitination of FANCD2. There is in vitro and in vivo evidence suggesting that at least some of the FA proteins also promote survival ..
  22. Twenty-first Annual Fanconi Anemia Research Fund Scientific Symposium
    Grover Bagby; Fiscal Year: 2009
    ..pathway," eight of the eleven known Fanconi anemia proteins bind together in a complex and monoubiquitinate FANCD2, one of the proteins not in the "core" complex...
  23. Zeiss 2-photon (2P) LSM780 laser scanning confocal microscope
    Moshe Levi; Fiscal Year: 2013
    ..Dynamic imaging of metabolism is achieved in part through monitoring changes in NAD(P)H and FAD lifetimes...
  24. Role of FA proteins in hematopoiesis
    Qishen Pang; Fiscal Year: 2013
    ..Our most recent identification of the FANCD2- FOXO3a complex and preliminary characterization of impaired anti-oxidant defense in primary BM cells from FA ..
  25. FANCD2 interaction with mismatch repair proteins and MCM2-7
    Gary M Kupfer; Fiscal Year: 2013
    ..The central hypothesis for the proposed research is that FANCD2 acts as a signal transduction protein to interact with downstream effectors such as the MSH2, the mismatch repair ..
  26. 23rd Annual Fanconi Anemia Research Fund Scientific Symposium
    GROVER CARLTON BAGBY; Fiscal Year: 2011
    ..pathway," eight of the eleven known Fanconi anemia proteins bind together in a complex and monoubiquitinate FANCD2, one of the proteins not in the "core" complex...
  27. Targeting Rad6 Postreplication DNA Repair to Treat Triple Negative Breast Cancer
    Malathy P V Shekhar; Fiscal Year: 2013
    ..Rad6 also regulates FA pathway activation by promoting FancD2 ubiquitination, a critical event for ICL repair...
  28. Searching for a New Fanconi Anemia-BRCA Pathway Gene on Chromosome 11p
    NIALL GEORGE HOWLETT; Fiscal Year: 2010
    ..A critical step in the activation of the FA-BRCA pathway is the mono- ubiquitination of the Fanconi anemia D2 (FANCD2) protein...
  29. Ras18-mediated Fanconi Anemia pathway activation in response to camptothecin
    Komaraiah Palle; Fiscal Year: 2013
    ..deficient cells or cells expressing mutant Rad18 defective in E3 ligase activity, fail to efficiently recruit FANCD2 (a key component of FA pathway activation) to chromatin making cells hypersensitive to CPT...
  30. Intrinsic fluorescence to guide characterization and purification of stem cells
    Brenda M Ogle; Fiscal Year: 2010
    ..We hypothesize and have preliminary data to suggest that intrinsic metabolic signatures (i.e., NADH, FAD) can serve as noninvasive biomarkers of stem cells when detected with multiphoton optical based approaches...
  31. Flavoproteins in Oxidative Protein Folding
    Colin Thorpe; Fiscal Year: 2013
    ..This application deals with several evolutionarily-related FAD-dependent sulfhydryl oxidases: members of the Quiescin-sulfhydryl oxidase (QSOX) family of flavoproteins, and a ..
  32. Regulation of the Mono-Ubiquitination of the Fanconi Anemia D2 Protein
    NIALL GEORGE HOWLETT; Fiscal Year: 2013
    ..A critical step in the activation of the FA-BRCA pathway is the mono-ubiquitinaton of the FANCD2 and FANCI proteins...
  33. Intravital Imaging of Stromal and Metabolic Changes in Mammary Tumors
    Pamela A Young; Fiscal Year: 2011
    ..we will collect fluorescence lifetime signatures of the intrinsically fluorescent metabolic co-factors, NADH and FAD, which are known to change based on the microenvironment of the cells...
  34. The Fanconi anemia pathway: role in DNA interstrand cross-link repair
    Johannes Walter; Fiscal Year: 2013
    ..by defects in thirteen Fanc proteins, eight of which form a ubiquitin ligase that mono-ubiquitylates the FancI-FancD2 heterodimer (the "ID" complex)...
  35. FANCD2 Monoubiquitination in DNA Damage Responses
    Paul R Andreassen; Fiscal Year: 2012
    ..A nuclear complex of at least 8 FA proteins is required for the monoubiquitination of FANCD2. Monoubiquitination of FANCD2 is required for resistance to the crosslinker mitomycin C...
  36. In-vivo optical imaging of neurovascular coupling and cerebral metabolism
    Elizabeth M C Hillman; Fiscal Year: 2012
    ..expressing fluorescent proteins, and intrinsically fluorescent substrates of energy metabolism such as NADH and FAD. We propose to refine and apply our imaging tools to address the following two fundamentally important questions: &..
  37. Mechanistic Dissection of the Fanconi Anemia Pathway of DNA Damage Response and R
    Patrick Sung; Fiscal Year: 2013
    ..interactions involving key FA and partner proteins mediate the post-translational modifications of the FANCI-FANCD2 complex and also the homology-directed repair of damaged DNA...
  38. Regulation of the Fanconi Anemia Pathway by Ubiquitination
    ALAN D D'ANDREA; Fiscal Year: 2013
    ..N Engl J Med 2010;362:1909-19). A critical downstream event in the FA pathway is the monubiquitination of the FANCD2/FANCI heterodimer...
  39. A Novel Role for the Fanconi Anemia Pathway in Replication of B[a]P-Adducted DNA
    Cyrus Vaziri; Fiscal Year: 2012
    ..The Specific Aims of this proposal are: (1) To determine the molecular basis for BPDE-induced interaction between FANCD2 (the putative effector of the FA pathway) and Pol kappa...
  40. Genomics of Coenzyme Metabolism in Bacterial Pathogens
    Andrei L Osterman; Fiscal Year: 2010
    ..to develop a comprehensive genomic analysis of three subsystems: (1) Coenzyme A, (2) NAD and NADP, and (3) FMN and FAD metabolism in a variety of bacterial pathogens and commensals...
  41. Role of Deubiquitination in Fanconi Anemia Cancer Susceptibility Pathway
    TONY TUNG HUANG; Fiscal Year: 2013
    ..Monoubiquitination of the FA protein FANCD2 appears to be critical in the repair of DNA damage because many of the proteins that are mutated in FA are ..
  42. Fanconi anemia pathway in DNA damage repair
    Junjie Chen; Fiscal Year: 2013
    ..The key function of the FA pathway is to promote FANCD2 monoubiquitination following DNA damage. However, how FANCD2 is recruited to sites of DNA damage is unknown...
  43. MOLECULAR PATHOGENESIS OF FANCONI ANEMIA
    ALAN D D'ANDREA; Fiscal Year: 2013
    ..grant are to (i) identify the molecular sensor of the FA/BRCA pathway (ii) determine the structural etements of FANCD2 required for its functional interaction with BRCA2 in chromatin and required for its DNA repair activities, and (..
  44. MicroRNAs Regulating The Fanconi Anemia-BRCA Pathway
    Toshiyasu Taniguchi; Fiscal Year: 2009
    ..Key proteins in the pathway, such as FANCD2 and RAD51, accumulate at sites of DNA damage and repair, form nuclear foci and regulate DNA repair...
  45. Epigenetic mechanisms relevant to the pathogenesis of ALS
    Neil W Kowall; Fiscal Year: 2013
    ..potential substrates for LSD1, a key enzyme regulating protein methylation that shares considerable homology with FAD-dependent polyamine oxidases...
  46. FAA AND FAD GENES AND PROTEINS
    Manuel Buchwald; Fiscal Year: 1999
    ..and confirmed its identity by the identification of mutations in FA-A cells; he also has a strong candidate for the FAD gene...
  47. Studies on p-Cresol Methylhydroxylase
    William McIntire; Fiscal Year: 2004
    ..be to study important aspects of the assembly of PCMH, particularly those relating to the mechanism of covalent FAD attachment to Tyr384 in the flavoprotein subunit...
  48. Defining the role of FANCD2 in homology-directed DNA Repair
    Steven Margossian; Fiscal Year: 2009
    ..A key step in this pathway is the monoubiquitination of FANCD2, resulting in its redistribution into nuclear foci...
  49. Functional Analysis of the Fanconi Pathway
    Maureen Hoatlin; Fiscal Year: 2009
    ..Current models suggest that a trigger point for the FA pathway is the activation of a downstream protein, FANCD2. A set of at least eight functionally-interdependent FA core complex proteins is indispensible for the function of ..
  50. Comparative effectiveness of Brain Imaging and Blood Biomarkers in Alzheimer??s d
    Orly Lazarov; Fiscal Year: 2010
    ..Our studies in mouse models of Familial Alzheimer's disease (FAD) revealed that neurogenesis is severely compromised in these mice...
  51. The Role of Peroxiredoxin1 &Reactive Oxygen Species in Breast Tumor Initiation
    Carola Anke Neumann; Fiscal Year: 2013
    ..sequence into mammary epithelial cells isolated from Prdx1-/-MMTV-ErbB-2 mice and transplant cells into clear fad pads of NCR nude mice, to observe if nuclear Prdx1 decreases incidence of breast cancer or tumor burden...
  52. Re-expression of Aberrantly Silenced Genes Induced by Polyamine Analogues
    Robert A Casero; Fiscal Year: 2013
    ..The first histone lysine demethylase discovered, LSD1, an FAD-dependent oxidase, targets mono- and dimethyl-lysine 4 histone 3 (H3K4me1 &H3K4me2)...
  53. The Molecular Physiology of Renalase
    Gary V Desir; Fiscal Year: 2010
    ..That effort led to the discovery of renalase, a novel FAD-dependent amine oxidase that is expressed in kidney, and heart...
  54. Structural/Functional Modularity in Nitric Oxide Synthase
    Linda J Roman; Fiscal Year: 2012
    ..are that the molecular design of the NOS isoforms, while requiring the same cofactors and prosthetic groups (FAD, FMN, Fe-protoporphyrin IX, Zn and tetrahydrobiopterin), is adapted in each isoform to satisfy its specific ..
  55. Impact of Presenilin 2 Mutation and Deficiency on Microglial Function
    Suman Jayadev; Fiscal Year: 2010
    ..neurodegenerative disease by characterizing the phenotype of microglia deficient in PS2 or expressing Familial AD (FAD) PS2 mutations in vitro...
  56. Presenilin1/g-secretase regulate miRNAs and neuronal survival
    Nikolaos K Robakis; Fiscal Year: 2013
    ..Reduction in brain glucose metabolism is also observed in familial AD (FAD) patients and may occur years before disease onset in carriers of presenilin-1 (PS1) FAD mutations implicating PS1 ..
  57. Presenilins and Cell Adhesion Molecules
    Angele Parent; Fiscal Year: 2012
    ..Interestingly, using PS1 M146V knock-in mice we find that expression of FAD-linked mutant also elevates cAMP-dependent signaling in cultured neurons, and leads to increased spine density in ..
  58. Therapeutic Exploitation of Mutant BRAF for Astrocytoma
    Peter J Houghton; Fiscal Year: 2013
    ..In BT-40 xenografts, MEK inhibition leads to rapid and complete loss of the DNA repair protein FANCD2, downstream of TORC1, thus potentially selectively sensitizing tumor cells to cisplatin, etoposide and ionizing ..
  59. Regulation of APP Pathway Gene Promoters in Alzheimer's
    Debomoy K Lahiri; Fiscal Year: 2011
    ..Cell lines from families with characterized FAD will be analyzed for differential expression of the APP and BACE genes...
  60. Presenilins and neuronal calcium dyshomeostasis
    ILYA B BEZPROZVANNY; Fiscal Year: 2013
    ..Missense mutations in presenilins account for 40% of familial AD (FAD) cases...
  61. Presenilin dysfunction in the brain
    Raymond J Kelleher; Fiscal Year: 2013
    ..in presenilin 1 (PS1) and presenilin 2 (PS2) account for ~90% of all identified causative mutations in familial AD (FAD), highlighting the importance of presenilins (PS) in AD pathogenesis...
  62. Mechanisms and Roles of Ca2+ Dysregulation in Alzheimer's Disease
    Dustin J Shilling; Fiscal Year: 2012
    ..important principles from studying a small population of patients that suffer from a genetic, familial form of AD (FAD), linked to mutations in the presenilin (PS) genes...
  63. STRUCTURAL DETERMINANTS OF FAD and FMN-REQUIRING ENZYMES
    Bettie Sue Masters; Fiscal Year: 2005
    This proposal emanates from the interests of the Principal Investigator in flavoproteins containing both FAD and FMN as prosthetic groups, extending from her early studies on NADPI-I-cytochrome P450 reductase (CYPOR) to the three nitric ..
  64. Peripheral Biomarkers in Familial Alzheimer's Disease
    Paul Coleman; Fiscal Year: 2009
    ..In this proposal we focus largely on the mutated gene for PS1 since it is a frequently mutated gene among the FAD gene mutations...
  65. RESONANCE RAMAN PROBE STUDIES OF FLAVOPROTEINS
    JAMES MC FARLAND; Fiscal Year: 1980
    ..acyl CoA dehydrogenase has proven important in investigation of hydrogen bonding between the N-3 hydrogen on the FAD and the protein. R.R...
  66. Clinical Applications of a Novel Fanconi Anemia Assay
    Akiko Shimamura; Fiscal Year: 2007
    ..She proposes to study the clinical applications of these findings as follows: 1. Investigate the FANCD activation assay as a new functional screen for the FA pathway, 2...