FANCD2

Summary

Gene Symbol: FANCD2
Description: Fanconi anemia, complementation group D2
Alias: FA-D2, FA4, FACD, FAD, FAD2, FANCD, Fanconi anemia group D2 protein
Species: human

Top Publications

  1. ncbi The intraflavin hydrogen bond in human electron transfer flavoprotein modulates redox potentials and may participate in electron transfer
    T M Dwyer
    Department of Pediatrics, Cell and Developmental Biology Program, University of Colorado School of Medicine, Denver 80262, USA
    Biochemistry 38:9735-45. 1999
  2. pmc ATR couples FANCD2 monoubiquitination to the DNA-damage response
    Paul R Andreassen
    Department of Radiation Oncology, Dana Farber Cancer Institute, Harvard Medical School, Boston, Massachusetts 02115, USA
    Genes Dev 18:1958-63. 2004
  3. ncbi A novel ubiquitin ligase is deficient in Fanconi anemia
    Amom Ruhikanta Meetei
    Laboratory of Genetics, National Institute on Aging, National Institutes of Health, 333 Cassell Drive, TRIAD Center Room 3000, Baltimore, Maryland 21224, USA
    Nat Genet 35:165-70. 2003
  4. ncbi Interaction of the Fanconi anemia proteins and BRCA1 in a common pathway
    I Garcia-Higuera
    Department of Pediatric Oncology, Dana Farber Cancer Institute, and Department of Pediatrics, Children s Hospital, Harvard Medical School, Boston, MA 02115, USA
    Mol Cell 7:249-62. 2001
  5. doi Deficiency of FANCD2-associated nuclease KIAA1018/FAN1 sensitizes cells to interstrand crosslinking agents
    Katja Kratz
    Institute of Molecular Cancer Research, University of Zurich, ETH Zurich, Winterthurerstrasse 190, 8057 Zurich, Switzerland
    Cell 142:77-88. 2010
  6. ncbi FANCI is a second monoubiquitinated member of the Fanconi anemia pathway
    Ashley E Sims
    Department of Biochemistry, New York University School of Medicine, New York, New York 10016, USA
    Nat Struct Mol Biol 14:564-7. 2007
  7. ncbi FANCC, FANCE, and FANCD2 form a ternary complex essential to the integrity of the Fanconi anemia DNA damage response pathway
    Susan M Gordon
    Program in Genetics and Genomic Biology, Research Institute, Hospital for Sick Children, Toronto, Ontario M5G 1X8, Canada
    J Biol Chem 280:36118-25. 2005
  8. ncbi The nuclear accumulation of the Fanconi anemia protein FANCE depends on FANCC
    France Léveillé
    Department of Clinical Genetics and Human Genetics, VU University Medical Center, Van der Boechorststraat 7, 1081 BT Amsterdam, The Netherlands
    DNA Repair (Amst) 5:556-65. 2006
  9. doi Ku70 corrupts DNA repair in the absence of the Fanconi anemia pathway
    Paul Pace
    MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK
    Science 329:219-23. 2010
  10. pmc A human ortholog of archaeal DNA repair protein Hef is defective in Fanconi anemia complementation group M
    Amom Ruhikanta Meetei
    Division of Experimental Hematology, Cincinnati Children s Hospital Research Foundation and University of Cincinnati College of Medicine, 3333 Burnet Avenue, Cincinnati, Ohio 45229, USA
    Nat Genet 37:958-63. 2005

Detail Information

Publications175 found, 100 shown here

  1. ncbi The intraflavin hydrogen bond in human electron transfer flavoprotein modulates redox potentials and may participate in electron transfer
    T M Dwyer
    Department of Pediatrics, Cell and Developmental Biology Program, University of Colorado School of Medicine, Denver 80262, USA
    Biochemistry 38:9735-45. 1999
    ..denitrificans ETFs determined by X-ray crystallography indicate that the 4'-hydroxyl of the ribityl side chain of FAD is hydrogen bonded to N(1) of the flavin ring...
  2. pmc ATR couples FANCD2 monoubiquitination to the DNA-damage response
    Paul R Andreassen
    Department of Radiation Oncology, Dana Farber Cancer Institute, Harvard Medical School, Boston, Massachusetts 02115, USA
    Genes Dev 18:1958-63. 2004
    ..The FA pathway regulates the monoubiquitination of FANCD2 and the assembly of damage-associated FANCD2 nuclear foci...
  3. ncbi A novel ubiquitin ligase is deficient in Fanconi anemia
    Amom Ruhikanta Meetei
    Laboratory of Genetics, National Institute on Aging, National Institutes of Health, 333 Cassell Drive, TRIAD Center Room 3000, Baltimore, Maryland 21224, USA
    Nat Genet 35:165-70. 2003
    ..1,2). A key step in this pathway is monoubiquitination of FANCD2, resulting in the redistribution of FANCD2 to nuclear foci containing BRCA1 (ref. 3)...
  4. ncbi Interaction of the Fanconi anemia proteins and BRCA1 in a common pathway
    I Garcia-Higuera
    Department of Pediatric Oncology, Dana Farber Cancer Institute, and Department of Pediatrics, Children s Hospital, Harvard Medical School, Boston, MA 02115, USA
    Mol Cell 7:249-62. 2001
    ..a nuclear complex containing the FANCA, FANCC, FANCF, and FANCG proteins is required for the activation of the FANCD2 protein to a monoubiquitinated isoform...
  5. doi Deficiency of FANCD2-associated nuclease KIAA1018/FAN1 sensitizes cells to interstrand crosslinking agents
    Katja Kratz
    Institute of Molecular Cancer Research, University of Zurich, ETH Zurich, Winterthurerstrasse 190, 8057 Zurich, Switzerland
    Cell 142:77-88. 2010
    ..The link of KIAA1018 to the FA pathway is further strengthened by its recruitment to DNA damage through interaction of its UBZ domain with monoubiquitylated FANCD2. We therefore propose to name KIAA1018 FANCD2-associated nuclease, FAN1.
  6. ncbi FANCI is a second monoubiquitinated member of the Fanconi anemia pathway
    Ashley E Sims
    Department of Biochemistry, New York University School of Medicine, New York, New York 10016, USA
    Nat Struct Mol Biol 14:564-7. 2007
    Activation of the Fanconi anemia (FA) DNA damage-response pathway results in the monoubiquitination of FANCD2, which is regulated by the nuclear FA core ubiquitin ligase complex...
  7. ncbi FANCC, FANCE, and FANCD2 form a ternary complex essential to the integrity of the Fanconi anemia DNA damage response pathway
    Susan M Gordon
    Program in Genetics and Genomic Biology, Research Institute, Hospital for Sick Children, Toronto, Ontario M5G 1X8, Canada
    J Biol Chem 280:36118-25. 2005
    ..of seven of the nine identified FA genes participate in a protein complex required for monoubiquitination of the FANCD2 protein...
  8. ncbi The nuclear accumulation of the Fanconi anemia protein FANCE depends on FANCC
    France Léveillé
    Department of Clinical Genetics and Human Genetics, VU University Medical Center, Van der Boechorststraat 7, 1081 BT Amsterdam, The Netherlands
    DNA Repair (Amst) 5:556-65. 2006
    ..component of the nuclear FA core complex, which is required for monoubiquitination of the downstream target FANCD2, an important step in the FA pathway of DNA cross-link repair...
  9. doi Ku70 corrupts DNA repair in the absence of the Fanconi anemia pathway
    Paul Pace
    MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK
    Science 329:219-23. 2010
    ..Ku70 binds directly to free DNA ends, committing them to NHEJ repair. We show that purified FANCD2, a downstream effector of the FA pathway, might antagonize Ku70 activity by modifying such DNA substrates...
  10. pmc A human ortholog of archaeal DNA repair protein Hef is defective in Fanconi anemia complementation group M
    Amom Ruhikanta Meetei
    Division of Experimental Hematology, Cincinnati Children s Hospital Research Foundation and University of Cincinnati College of Medicine, 3333 Burnet Avenue, Cincinnati, Ohio 45229, USA
    Nat Genet 37:958-63. 2005
    ..Each protein in this complex is essential for monoubiquitination of FANCD2, a key reaction in the Fanconi anemia DNA damage-response pathway...
  11. ncbi S-phase-specific interaction of the Fanconi anemia protein, FANCD2, with BRCA1 and RAD51
    Toshiyasu Taniguchi
    Department of Pediatric Oncology, Dana Farber Cancer Institute, Harvard Medical School, Boston, MA 02115, USA
    Blood 100:2414-20. 2002
    ..DNA damage activates this pathway, leading to monoubiquitination of the downstream FANCD2 protein and targeting to nuclear foci containing BRCA1...
  12. ncbi Convergence of the fanconi anemia and ataxia telangiectasia signaling pathways
    Toshiyasu Taniguchi
    Department of Pediatric Oncology, Dana Farber Cancer Institute and Department of Pediatrics, Children s Hospital, Harvard Medical School, Boston, MA 02115, USA
    Cell 109:459-72. 2002
    ..Here, we identify the Fanconi anemia protein, FANCD2, as a link between the FA and ATM damage response pathways. ATM phosphorylates FANCD2 on serine 222 in vitro...
  13. ncbi Fanconi anemia protein complex: mapping protein interactions in the yeast 2- and 3-hybrid systems
    Susan M Gordon
    Program in Genetics and Genomic Biology, Research Institute, Hospital for Sick Children, 555 University Avenue, Toronto, Ontario M5G 1X8, Canada
    Blood 102:136-41. 2003
    ..The FANCA, FANCC, FANCE, FANCF, and FANCG proteins form a nuclear complex required for the monoubiquination of the FANCD2 protein...
  14. pmc FANCI phosphorylation functions as a molecular switch to turn on the Fanconi anemia pathway
    Masamichi Ishiai
    Laboratory of DNA Damage Signaling, Department of Late Effect Studies, Radiation Biology Center, Kyoto University, Yoshidakonoe cho, Sakyo ku, Kyoto 606 8501, Japan
    Nat Struct Mol Biol 15:1138-46. 2008
    ..DNA damage or replication fork stress, the Fanconi anemia pathway is activated, leading to monoubiquitination of FANCD2 and FANCI and their colocalization in foci...
  15. pmc Human Fanconi anemia monoubiquitination pathway promotes homologous DNA repair
    Koji Nakanishi
    Molecular Biology Program, Memorial Sloan Kettering Cancer Center, 1275 York Avenue, New York, NY 10021, USA
    Proc Natl Acad Sci U S A 102:1110-5. 2005
    ..DNA-damaging agents activate the monoubiquitination of FANCD2, resulting in its targeting to nuclear foci that also contain BRCA1 and BRCA2/FANCD1, proteins involved in ..
  16. pmc A role for monoubiquitinated FANCD2 at telomeres in ALT cells
    Qiang Fan
    Division of Experimental Hematology and Cancer Biology, Cincinnati Children s Research Foundation, Department of Pediatrics, University of Cincinnati College of Medicine, Cincinnati, OH 45229, USA
    Nucleic Acids Res 37:1740-54. 2009
    ..Because of these similarities, we have investigated whether there is a relationship between the FA protein, FANCD2 and telomeres...
  17. ncbi BRCA1-independent ubiquitination of FANCD2
    Cassandra J Vandenberg
    Protein and Nucleic Acid Chemistry Division, MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, United Kingdom
    Mol Cell 12:247-54. 2003
    Monoubiquitination of the FANCD2 protein is a key step in the Fanconi anemia (FA) tumor suppressor pathway, coinciding with this molecule's accumulation at sites of genome damage...
  18. pmc Functional interaction of monoubiquitinated FANCD2 and BRCA2/FANCD1 in chromatin
    XiaoZhe Wang
    Department of Pediatric Oncology, Dana Farber Cancer Institute, Harvard Medical School, Boston, Massachusetts 02115, USA
    Mol Cell Biol 24:5850-62. 2004
    ..Here we show that monoubiquitination of FANCD2, which is activated by DNA damage, is required for targeting of FANCD2 to chromatin, where it interacts with BRCA2...
  19. pmc Fanconi anemia protein FANCD2 inhibits TRF1 polyADP-ribosylation through tankyrase1-dependent manner
    Alex Lyakhovich
    Department of Genetics and Microbiology, Universitat Autonoma de Barcelona, Edifici C, Bellaterra, Barcelona 08193, Spain
    Genome Integr 2:4. 2011
    ..abstract:..
  20. ncbi X-linked inheritance of Fanconi anemia complementation group B
    Amom Ruhikanta Meetei
    Laboratory of Genetics, National Institute on Aging, National Institutes of Health, 333 Cassell Drive, TRIAD Center Room 3000, Baltimore, Maryland 21224, USA
    Nat Genet 36:1219-24. 2004
    ..group B is an essential component of the nuclear protein 'core complex' responsible for monoubiquitination of FANCD2, a key event in the DNA-damage response pathway associated with Fanconi anemia and BRCA...
  21. pmc HPV-16 E7 reveals a link between DNA replication stress, fanconi anemia D2 protein, and alternative lengthening of telomere-associated promyelocytic leukemia bodies
    Nicole Spardy
    Biochemistry and Molecular Genetics Graduate Program, and Department of Pathology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15213, USA
    Cancer Res 68:9954-63. 2008
    ..proteins that are involved in the response to DNA replication stress, most notably the Fanconi anemia D2 protein (FANCD2) as well as BRCA2 and MUS81...
  22. pmc Histone H2AX and Fanconi anemia FANCD2 function in the same pathway to maintain chromosome stability
    Massimo Bogliolo
    Group of Mutagenesis, Department of Genetics and Microbiology, Universitat Autonoma de Barcelona, Bellaterra, Barcelona, Spain
    EMBO J 26:1340-51. 2007
    ..The central FA protein FANCD2 is known to relocate to chromatin upon DNA damage in a poorly understood process...
  23. ncbi The WD40 repeats of FANCL are required for Fanconi anemia core complex assembly
    Allan M Gurtan
    Biological and Biomedical Sciences Program, Dana Farber Cancer Institute, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Biol Chem 281:10896-905. 2006
    ..form a multisubunit nuclear complex (FA core complex) required for monoubiquitination of a downstream FA protein, FANCD2. FANCL, which possesses three WD40 repeats and a plant homeodomain (PHD), is the putative E3 ubiquitin ligase ..
  24. ncbi Direct DNA binding activity of the Fanconi anemia D2 protein
    Woo Hyun Park
    Department of Pathology, Brigham and Women s Hospital and Harvard Medical School, Boston, Massachusetts 02115, USA
    J Biol Chem 280:23593-8. 2005
    ..In these experiments we purified full-length Fanconi anemia protein D2 (FANCD2), and we found that FANCD2 bound to DNA with specificity for certain structures: double strand DNA ends and ..
  25. ncbi FANCD2 protein is expressed in proliferating cells of human tissues that are cancer-prone in Fanconi anaemia
    Michael Hölzel
    Department of Pathology, VU University Medical Center, Amsterdam, The Netherlands
    J Pathol 201:198-203. 2003
    ..At least seven distinct FA proteins function in concert to protect the genome, a key step being the activation of FANCD2 by mono-ubiquitination...
  26. ncbi Positional cloning of a novel Fanconi anemia gene, FANCD2
    C Timmers
    Department of Molecular and Medical Genetics, Oregon Health Sciences University, Portland, OR 97201, USA
    Mol Cell 7:241-8. 2001
    ..Complementation group D is heterogeneous, consisting of two distinct genes, FANCD1 and FANCD2. Here we report the positional cloning of FANCD2...
  27. ncbi Direct interaction of FANCD2 with BRCA2 in DNA damage response pathways
    Shobbir Hussain
    Division of Genetics and Development, Guy s, King s and St Thomas s School of Medicine, King s College London, UK
    Hum Mol Genet 13:1241-8. 2004
    ..proteins encoded by the known FA genes form a nuclear complex which is required for the monoubiquitination of the FANCD2 protein...
  28. pmc FANCE: the link between Fanconi anaemia complex assembly and activity
    Paul Pace
    MRC Laboratory of Molecular Biology, Hills Road, Cambridge, UK
    EMBO J 21:3414-23. 2002
    ..It activates the downstream protein FANCD2 by monoubiquitylation; this then forges an association with the BRCA1 protein at sites of DNA damage...
  29. ncbi Regulated interaction of the Fanconi anemia protein, FANCD2, with chromatin
    Rocio Montes De Oca
    Dana Farber Cancer Institute, Department of Radiation Oncology, Harvard Medical School, 44 Binney St, Boston, MA 02115, USA
    Blood 105:1003-9. 2005
    DNA damage activates the monoubiquitination of the Fanconi anemia (FA) protein, FANCD2, resulting in the assembly of FANCD2 nuclear foci...
  30. pmc Localization of the Fanconi anemia complementation group D gene to a 200-kb region on chromosome 3p25.3
    J A Hejna
    Department of Molecular and Medical Genetics, Oregon Health Sciences University, Portland, OR 97201, USA
    Am J Hum Genet 66:1540-51. 2000
    ..The FA complementation group D gene (FANCD) has previously been localized to chromosome 3p22-26, by use of microcell-mediated chromosome transfer...
  31. ncbi The Fanconi anemia pathway is required for the DNA replication stress response and for the regulation of common fragile site stability
    Niall G Howlett
    Department of Human Genetics, University of Michigan, Ann Arbor, MI 48109, USA
    Hum Mol Genet 14:693-701. 2005
    ..damaging agents, and during S-phase of the cell cycle, the FA pathway is activated via the mono-ubiquitination of FANCD2 (FANCD2-Ub), signaling its translocation to discrete nuclear foci, where it co-localizes with the central DNA ..
  32. ncbi Xanthine oxidase and xanthine dehydrogenase from an estivating land snail
    M Hermes Lima
    Institute of Biochemistry, Carleton University, Ottawa, Ontario, Canada
    Z Naturforsch C 50:685-94. 1995
    ..that the formation of an enzyme-methylene blue complex induces protection against heat and oxidative damage at the FAD-active site...
  33. ncbi Impaired non-esterified fatty acid suppression is associated with endothelial dysfunction in insulin resistant subjects
    B M Balletshofer
    Department of Endocrinology and Metabolism, , Germany
    Horm Metab Res 33:428-31. 2001
    ..Endothelial function was measured as flow-associated vasodilation (FAD%) of the brachial artery...
  34. pmc Rad18 E3 ubiquitin ligase activity mediates Fanconi anemia pathway activation and cell survival following DNA Topoisomerase 1 inhibition
    Komaraiah Palle
    University of North Carolina, Chapel Hill, NC USA
    Cell Cycle 10:1625-38. 2011
    ..We show here that the FA core complex protein FANCA and monoubiquitinated FANCD2 (an effector of the FA pathway) are rapidly mobilized to chromatin in response to CPT treatment in several human ..
  35. ncbi Crystal structure of Escherichia coli thioredoxin reductase refined at 2 A resolution. Implications for a large conformational change during catalysis
    G Waksman
    Laboratory of Molecular Biophysics, Rockefeller University, New York, NY 10021
    J Mol Biol 236:800-16. 1994
    ..adenine dinucleotide phosphate (NADPH) to a disulfide bond in the enzyme, via a flavin adenine dinucleotide (FAD)...
  36. ncbi Blue light mediated photoreduction of the flavoprotein NADPH-cytochrome P450 reductase. A Förster-type energy transfer
    D Muller-Enoch
    Abteilung Physiologische Chemie, Universitat Ulm, Germany
    Z Naturforsch C 52:605-14. 1997
    The absorption spectra and the corresponding molar absorption coefficients of the fluorophores umbelliferone, FAD and FMN and of the FAD and FMN containing flavoprotein NADPH-cytochrome P450 reductase of different oxydation-reduction ..
  37. ncbi Regulation of nitrogen fixation in Klebsiella pneumoniae and Azotobacter vinelandii: NifL, transducing two environmental signals to the nif transcriptional activator NifA
    Ruth A Schmitz
    Institut fur Mikrobiologie und Genetik, Gottingen, Germany
    J Mol Microbiol Biotechnol 4:235-42. 2002
    ..of the negative regulator NifL appears to lie in the C-terminal domain, whereas the N-terminal domain binds FAD as a redox-sensitive cofactor, which is required for signal transduction of the internal oxygen status...
  38. ncbi Genes and mechanisms involved in beta-amyloid generation and Alzheimer's disease
    H Steiner
    Adolf-Butenandt-Institute, Ludwig-Maximilians-University, Dept. of Biochemistry, Munich, Germany
    Eur Arch Psychiatry Clin Neurosci 249:266-70. 1999
    ..In some very rare cases of familial AD (FAD), mutations have been identified within the betaAPP gene...
  39. ncbi FANCD2 Western blot as a diagnostic tool for Brazilian patients with Fanconi anemia
    D V Pilonetto
    Laboratorio de Imunogenetica, Hospital de Clinicas, Universidade Federal do Parana, Curitiba, PR, Brasil
    Braz J Med Biol Res 42:237-43. 2009
    ..in molecular pathogenesis of this disease led researchers to develop a diagnostic test based on Western blot for FANCD2. The objective of the present study was to determine the efficacy of this method for the diagnosis of 84 Brazilian ..
  40. ncbi Synergistic interactions of multiple mutations on catalysis during the hydroxylation reaction of p-hydroxybenzoate hydroxylase: studies of the Lys297Met, Asn300Asp, and Tyr385Phe mutants reconstituted with 8-Cl-flavin
    M Ortiz Maldonado
    Department of Biological Chemistry, University of Michigan, Ann Arbor, Michigan 48109 0606
    Biochemistry 40:8705-16. 2001
    ..We have used wild-type (WT) PHBH and several mutant forms, all with normal FAD and with 8-Cl-FAD substituted for FAD, to assess the relative contributions of the two effects...
  41. pmc Inhibitory actions of diphenyleneiodonium on endothelium-dependent vasodilatations in vitro and in vivo
    Y X Wang
    Department of Pharmacology and Therapeutics, Faculty of Medicine, University of British Columbia, Vancouver, Canada
    Br J Pharmacol 110:1232-8. 1993
    ..effect of DPI (3 x 10(-7) M) on ACh-induced relaxation was prevented by pretreatment with NADPH (5 x 10(-3) M) and FAD (5 x 10(-4) M) but not L-arginine (L-Arg, 2 x 10(-3) M)...
  42. ncbi Global effects of the energetics of coenzyme binding: NADPH controls the protein interaction properties of human cytochrome P450 reductase
    Alex Grunau
    Department of Biochemistry, University of Leicester, UK
    Biochemistry 45:1421-34. 2006
    The thermodynamics of coenzyme binding to human cytochrome P450 reductase (CPR) and its isolated FAD-binding domain have been studied by isothermal titration calorimetry...
  43. doi Facile oxidation of leucomethylene blue and dihydroflavins by artemisinins: relationship with flavoenzyme function and antimalarial mechanism of action
    Richard K Haynes
    Department of Chemistry, Institute of Molecular Technology for Drug Discovery and Synthesis, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, P R China
    ChemMedChem 5:1282-99. 2010
    ..Dihydroflavins generated from riboflavin (RF) and FAD by pretreatment with sodium dithionite are rapidly oxidised by artemisinin to the parent flavins...
  44. pmc Genomic instability in mice is greater in Fanconi anemia caused by deficiency of Fancd2 than Fancg
    Ramune Reliene
    Cancer Research Center, Department of Environmental Health Sciences, University at Albany, State University of New York, Albany, New York, USA
    Cancer Res 70:9703-10. 2010
    ..FANCG is a part of the FA core complex that is responsible for monoubiquitination of FANCD2 and FANCI...
  45. pmc Electron transfer flavoprotein domain II orientation monitored using double electron-electron resonance between an enzymatically reduced, native FAD cofactor, and spin labels
    Michael A Swanson
    Department of Chemistry and Biochemistry, University of Denver, Denver, Colorado 80208, USA
    Protein Sci 20:610-20. 2011
    ..structure of human ETF bound to medium chain acyl-CoA dehydrogenase indicates that the flavin adenine dinucleotide (FAD) domain (αII) is mobile, which permits more rapid electron transfer with donors and acceptors by providing closer ..
  46. ncbi Gene-specific selection against experimental fanconi anemia gene inactivation in human cancer
    Eike Gallmeier
    The Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins University, Baltimore, Maryland, USA
    Cancer Biol Ther 6:654-60. 2007
    ..the sequential homozygous deletion was achieved only for FANCC and FANCG, acting upstream, but not for BRCA2 or FANCD2, acting downstream in the FA pathway...
  47. ncbi A novel role for Fanconi anemia (FA) pathway effector protein FANCD2 in cell cycle progression of untransformed primary human cells
    Ihn Young Song
    Department of Genetics and Genomics, Boston University School of Medicine, Boston, MA, USA
    Cell Cycle 9:2375-88. 2010
    ..The Fanconia Anemia D2 (FANCD2) protein is implicated in repair of various forms of DNA damage including ICLs...
  48. ncbi The Fanconi anemia protein, FANCE, promotes the nuclear accumulation of FANCC
    Toshiyasu Taniguchi
    Department of Pediatric Oncology, Dana Farber Cancer Institute, and the Department of Pediatrics, Children s Hospital, Harvard Medical School, Boston, MA 02115, USA
    Blood 100:2457-62. 2002
    ..The 6 known Fanconi anemia gene products (FANCA, FANCC, FANCD2, FANCE, FANCF, and FANCG proteins) interact in a common pathway...
  49. ncbi Oxygen reactions in p-hydroxybenzoate hydroxylase utilize the H-bond network during catalysis
    Mariliz Ortiz-Maldonado
    Department of Biological Chemistry, University of Michigan, Ann Arbor, Michigan 48109 0606, USA
    Biochemistry 43:15246-57. 2004
    ..a flavoprotein monooxygenase that catalyses a reaction in two parts: reduction of the flavin adenine dinucleotide (FAD) in the enzyme by reduced nicotinamide adenine dinucleotide phosphate (NADPH) in response to binding p-..
  50. pmc Monoketone analogs of curcumin, a new class of Fanconi anemia pathway inhibitors
    Igor Landais
    Department of Biochemistry and Molecular Biology, Oregon Health and Science University, Portland, USA
    Mol Cancer 8:133. 2009
    ..Thus, inhibition of FANCD2 monoubiquitylation (FANCD2-Ub), a key step in the FA pathway, might target tumor cells defective in ATM through ..
  51. doi UBE2W interacts with FANCL and regulates the monoubiquitination of Fanconi anemia protein FANCD2
    Yingying Zhang
    Laboratory of Protein Engineering, Institute of Biotechnology, Beijing, China
    Mol Cells 31:113-22. 2011
    ..disease mostly caused by improper regulation of the monoubiquitination of Fanconi anemia complementation group D2 (FANCD2)...
  52. ncbi [Doppler evaluation of blood flow of the lower limb]
    X Chanudet
    , ,
    Arch Mal Coeur Vaiss 83:1169-74. 1990
    ..BFLL estimation depends on common femoral artery diameter (FAD) echographic measurement at the thigh root and on velocity time interval (VTI) calculation with pulsed wave doppler ..
  53. ncbi Characterization of the flavoprotein moieties of NADPH-sulfite reductase from Salmonella typhimurium and Escherichia coli. Physicochemical and catalytic properties, amino acid sequence deduced from DNA sequence of cysJ, and comparison with NADPH-cytochrom
    J Ostrowski
    Howard Hughes Medical Institute Laboratory, Duke University Medical Center, Durham, North Carolina 27710
    J Biol Chem 264:15796-808. 1989
    ..and deduced amino acid sequences indicate that SiR-FP is an octamer of identical 66-kDa peptides and contains 4 FAD and 4 FMN per octamer...
  54. ncbi Histidine 61: an important heme ligand in the soluble fumarate reductase from Shewanella frigidimarina
    Emma L Rothery
    School of Chemistry, University of Edinburgh, UK
    Biochemistry 42:13160-9. 2003
    ..Biol. 6, 1108-1112] shows the presence of four, bis-His-ligated, c-type hemes and one flavin adenine dinucleotide, FAD. The heme groups provide a "molecular wire" for the delivery of electrons to the FAD...
  55. ncbi X-ray structure of the ferredoxin:NADP+ reductase from the cyanobacterium Anabaena PCC 7119 at 1.8 A resolution, and crystallographic studies of NADP+ binding at 2.25 A resolution
    L Serre
    IBS LCCP, Grenoble, France
    J Mol Biol 263:20-39. 1996
    ..172. The model includes 303 residues, the flavin adenine dinucleotide cofactor (FAD), one sulfate ion located at the putative NADP+ binding site and 328 water molecule sites...
  56. ncbi Heterozygosity for p53 (Trp53+/-) accelerates epithelial tumor formation in fanconi anemia complementation group D2 (Fancd2) knockout mice
    Scott Houghtaling
    Department of Molecular and Medical Genetics, Oregon Health and Science University, Portland, OR 97239, USA
    Cancer Res 65:85-91. 2005
    ..We have previously reported an increased incidence of epithelial tumors in Fancd2 knockout mice...
  57. ncbi Small cerebral vessel disease in familial amyloid and non-amyloid angiopathies: FAD-PS-1 (P117L) mutation and CADASIL. Immunohistochemical and ultrastructural studies
    Grazyna M Szpak
    Department of Neuropathology, Institute of Psychiatry and Neurology, Sobieskiego 9, 02 957 Warsaw, Poland
    Folia Neuropathol 45:192-204. 2007
    ..Two cases, previously diagnosed as familial Alzheimer's disease (FAD) with cerebral amyloid angiopathy (CAA), were confirmed by genetic and neuropathological studies, and one case of ..
  58. ncbi Steady state and picosecond time-resolved fluorescence studies on native, desulpho and deflavo xanthine oxidase
    A K Sau
    Department of Chemical Sciences, Tata Institute of Fundamental Research, Homi Bhabha Road, Colaba, 400 005, Mumbai, India
    Biochim Biophys Acta 1481:273-82. 2000
    ..replacement of the molybdenum double bonded sulphur by oxygen and the removal of the flavin adenine dinucleotide (FAD)...
  59. ncbi Efficacy and safety of selamectin against fleas and heartworms in dogs and cats presented as veterinary patients in North America
    M G Boy
    Animal Health Clinical Affairs, Central Research Division, Pfizer Inc, Groton, CT 06340, USA
    Vet Parasitol 91:233-50. 2000
    ..made on the beneficial effect of selamectin treatment on dogs and cats showing signs of flea allergy dermatitis (FAD)...
  60. ncbi Purification of electron-transferring flavoprotein from Megasphaera elsdenii and binding of additional FAD with an unusual absorption spectrum
    Kyosuke Sato
    Department of Molecular Physiology, Graduate School of Medical Sciences, Kumamoto University, 1 1 1 Honjo, Kumamoto, Kumamoto, 860 8556
    J Biochem 134:719-29. 2003
    ..The purified ETF contained one mol FAD/mol ETF as the sole non-protein component and bound almost one mol of additional FAD...
  61. ncbi Functional restoration. Returning patients with chronic low back pain to work--revolution or fad?
    R W Teasell
    Department of Physical Medicine and Rehabilitation, University Hospital, London, Ontario, Canada
    Spine (Phila Pa 1976) 21:844-7. 1996
    ..A review was performed to assess the basis for functional restoration programs in returning patients with chronic low back pain to work...
  62. pmc Significance of the Fanconi anemia FANCD2 protein in sporadic and metastatic human breast cancer
    Philip S Rudland
    Cancer and Polio Research Fund Laboratories, School of Biological Sciences, Biosciences Building, Crown Street, University of Liverpool, Liverpool, L69 3BX, United Kingdom
    Am J Pathol 176:2935-47. 2010
    b>FANCD2, a pivotal protein in the Fanconi anemia and BRCA pathway/network, is monoubiquitylated in the nucleus in response to DNA damage...
  63. pmc Cloning and characterization of FAD1, the structural gene for flavin adenine dinucleotide synthetase of Saccharomyces cerevisiae
    M Wu
    Department of Biological Sciences, Columbia University, New York, New York 10027
    Mol Cell Biol 15:264-71. 1995
    ..Mutants in this group display a reduced level of flavin adenine dinucleotide (FAD) and an increased level of flavin mononucleotide (FMN) in mitochondria...
  64. ncbi Purification and characterisation of the NADH:acceptor reductase component of xylene monooxygenase encoded by the TOL plasmid pWW0 of Pseudomonas putida mt-2
    J P Shaw
    Department of Medical Biochemistry, University Medical Center, Geneve, Switzerland
    Eur J Biochem 209:51-61. 1992
    ..The enzyme was found to contain 1 mol/mol of tightly but not covalently bound FAD, as well as 2 mol/mol of non-haem iron and 2 mol/mol of acid-labile sulfide, suggesting the presence of two redox ..
  65. ncbi Cytochrome b5 reductase: the roles of the recessive congenital methemoglobinemia mutants P144L, L148P, and R159*
    C Ainsley Davis
    Department of Biochemistry and Molecular Biology, College of Medicine, University of South Florida, Tampa, FL 33612, USA
    Arch Biochem Biophys 431:233-44. 2004
    ..of the primary sequence composed of residues G143 to V171 which serves as a "hinge" or "linker" region between the FAD- and NADH-binding lobes of the protein...
  66. ncbi Spectroscopic properties of Escherichia coli UDP-N-acetylenolpyruvylglucosamine reductase
    M J Axley
    Enzymology Laboratory, Bristol Myers Squibb Pharmaceutical Research Institute, Princeton, New Jersey 08543 4000, USA
    Biochemistry 36:812-22. 1997
    ..and UV-visible spectroscopy to establish the spectral properties of its tightly bound flavin adenine dinucleotide (FAD) cofactor...
  67. ncbi Low mitochondrial glycerophosphate dehydrogenase activity in lymphocytes of women with gestational diabetes
    J Vidal
    Endocrinology Unit, Hospital Clinic, Barcelona, Spain
    Horm Metab Res 29:60-2. 1997
    An abnormally low activity of mitochondrial FAD-linked glycerophosphate dehydrogenase (m-GDH), relative to the paired measurement of glutamate dehydrogenase, was found in CD3+ lymphocytes from 4 out of 14 mothers with gestational ..
  68. ncbi A critical review of cellobiose dehydrogenases
    G Henriksson
    Department of Pulp and Paper Chemistry and Technology, Royal Institute of Technology, 100 44, Stockholm, Sweden
    J Biotechnol 78:93-113. 2000
    ..CDH that adsorbs strongly and specifically to cellulose carries two prosthetic groups; namely, an FAD and a heme in two different domains that can be separated after limited proteolysis...
  69. ncbi Structure of alpha-glycerophosphate oxidase from Streptococcus sp.: a template for the mitochondrial alpha-glycerophosphate dehydrogenase
    Timothy Colussi
    Center for Structural Biology, Wake Forest University School of Medicine, Winston Salem, North Carolina 27157, USA
    Biochemistry 47:965-77. 2008
    The FAD-dependent alpha-glycerophosphate oxidase (GlpO) from Enterococcus casseliflavus and Streptococcus sp...
  70. doi A fluorescence perspective on the differential interaction of riboflavin and flavin adenine dinucleotide with cucurbit[7]uril
    Sharmistha Dutta Choudhury
    Radiation and Photochemistry Division, Bhabha Atomic Research Centre, Trombay, Mumbai 400 085, India
    J Phys Chem B 114:10717-27. 2010
    ..the macrocyclic host, cucurbit[7]uril (CB7), with riboflavin (RF) and its derivative, flavin adenine dinucleotide (FAD), has been investigated using absorption and steady-state and time-resolved fluorescence measurements...
  71. doi [Determinants of condom use and heterosexual multiple sexual partnership in French Antilles and French Guiana]
    A Cobat
    Observatoire régional de santé d Ile de France, Paris, France
    Rev Epidemiol Sante Publique 56:143-57. 2008
    ..The French-American departments (Guadeloupe, Martinique, Guiana), FAD, are located in the heart of this region...
  72. ncbi Binding of FAD to cytochrome b558 is facilitated during activation of the phagocyte NADPH oxidase, leading to superoxide production
    Shukichi Hashida
    Department of Structural Biology, Graduate School of Pharmaceutical Sciences, Hokkaido University, Sapporo 060 0812
    J Biol Chem 279:26378-86. 2004
    ..The activity of NADPH oxidase is dependent on FAD, but the physiological status of FAD in the oxidase is not fully elucidated...
  73. ncbi The flavoprotein component of the Escherichia coli sulfite reductase: expression, purification, and spectral and catalytic properties of a monomeric form containing both the flavin adenine dinucleotide and the flavin mononucleotide cofactors
    M Zeghouf
    Laboratoire de Chimie et Biochimie des Centres Redox Biologiques, CEA Grenoble, DBMS CBCRB CNRS Université Joseph Fourier, France
    Biochemistry 37:6114-23. 1998
    The flavoprotein component (SiR-FP) of the sulfite reductase from Escherichia coli is an octamer containing one FAD and one FMN per polypeptide chain. SiR-FP60, a SiR-FP fragment starting with alanine-52, was overexpressed in E...
  74. ncbi Four crystal structures of the 60 kDa flavoprotein monomer of the sulfite reductase indicate a disordered flavodoxin-like module
    A Gruez
    Laboratoire de Cristallographie et Cristallogenèse des Protéines Institut de Biologie Structurale J P Ebel, CEA CNRS, Grenoble, France
    J Mol Biol 299:199-212. 2000
    ..Each beta-subunit contains a Fe4S4 cluster and a siroheme, and each alpha-subunit binds one FAD and one FMN as prosthetic groups...
  75. ncbi Mechanism of coenzyme binding to human methionine synthase reductase revealed through the crystal structure of the FNR-like module and isothermal titration calorimetry
    Kirsten R Wolthers
    Faculty of Life Sciences, University of Manchester, Manchester Interdisciplinary Biocentre, 131 Princess Street, Manchester M1 7DN, United Kingdom
    Biochemistry 46:11833-44. 2007
    ..MSR contains one FAD and one FMN cofactor per polypeptide and functions in the sequential transfer of reducing equivalents from NADPH to ..
  76. pmc The iron-sulfur cluster of electron transfer flavoprotein-ubiquinone oxidoreductase is the electron acceptor for electron transfer flavoprotein
    Michael A Swanson
    Department of Chemistry and Biochemistry, University of Denver, Denver, Colorado 80208, USA
    Biochemistry 47:8894-901. 2008
    ..It contains one [4Fe-4S] (2+,1+) and one FAD, which are diamagnetic in the isolated oxidized enzyme and can be reduced to paramagnetic forms by enzymatic donors ..
  77. ncbi Relaxation kinetics of cytochrome P450 reductase: internal electron transfer is limited by conformational change and regulated by coenzyme binding
    Aldo Gutierrez
    Biological NMR Centre, University of Leicester, Medical Sciences Building, P O Box 138, University Road, Leicester LE1 9HN, UK
    Biochemistry 41:4626-37. 2002
    ..electron/hydride transfer and is consistent with its origin in local conformational change in the vicinity of the FAD isoalloxazine ring...
  78. ncbi Evaluation of a 15-day screening assay using intact male rats for identifying steroid biosynthesis inhibitors and thyroid modulators
    John C O'Connor
    DuPont Haskell Laboratory for Health and Environmental Sciences, P O Box 50, Newark, Delaware 19714, USA
    Toxicol Sci 69:79-91. 2002
    ..The test compounds included the aromatase inhibitor fadrozole (FAD), the testosterone biosynthesis inhibitor ketoconazole (KETO), and the thyroid modulators phenobarbital (PB) and ..
  79. ncbi The Na+-translocating NADH:ubiquinone oxidoreductase from Vibrio alginolyticus--redox states of the FAD prosthetic group and mechanism of Ag+ inhibition
    J Steuber
    ETH Zentrum, Zurich, Switzerland
    Eur J Biochem 249:770-6. 1997
    The FAD prosthetic group of the Na+-motive NADH:ubiquinone oxidoreductase (Na+-NQR) from Vibrio alginolyticus was investigated by ultraviolet-visible and fluorescence spectroscopy...
  80. pmc Role of salicylic acid and fatty acid desaturation pathways in ssi2-mediated signaling
    Pradeep Kachroo
    Department of Plant Pathology, University of Kentucky, Lexington, Kentucky 40546, USA
    Plant Physiol 139:1717-35. 2005
    ..Furthermore, the act1-mediated rescue of ssi2 phenotypes is also independent of the FAD2, FAD3, FAD4, FAD5, FAD7, and DGD1 genes...
  81. ncbi Crystal structure of a novel FAD-, FMN-, and ATP-containing L-proline dehydrogenase complex from Pyrococcus horikoshii
    Hideaki Tsuge
    Institute for Health Sciences, Tokushima Bunri University, 180 Nishihama bouji, Yamashiro cho, Tokushima 770 8514, Japan
    J Biol Chem 280:31045-9. 2005
    ..crystal structure of PDH1, which is a heterooctameric complex (alphabeta)4 containing three different cofactors: FAD, FMN, and ATP. The structure was determined by x-ray crystallography to a resolution of 2.86 angstroms...
  82. ncbi Characterization of the FAD-containing N-methyltryptophan oxidase from Escherichia coli
    P Khanna
    Department of Biochemistry, MCP Hahnemann School of Medicine, Philadelphia, Pennsylvania 19129, USA
    Biochemistry 40:1441-50. 2001
    ..The pK(a) for ionization of oxidized FAD at N(3)H in MTOX (8.36) is two pH units lower than that observed for free FAD...
  83. ncbi AppA, a redox regulator of photosystem formation in Rhodobacter sphaeroides 2.4.1, is a flavoprotein. Identification of a novel fad binding domain
    M Gomelsky
    Department of Microbiology and Molecular Genetics, University of Texas Medical School, Houston, Texas 77030, USA
    J Biol Chem 273:35319-25. 1998
    ..AppA was produced as insoluble inclusion bodies. The purified inclusion bodies were found to contain FAD. By overexpressing various deletion derivatives, we were able to localize the region of AppA sufficient for FAD ..
  84. ncbi Cytochrome b5 reductase: role of the si-face residues, proline 92 and tyrosine 93, in structure and catalysis
    Christopher C Marohnic
    Department of Biochemistry and Molecular Biology, College of Medicine, University of South Florida, Tampa, Florida 33612, USA
    Biochemistry 44:2449-61. 2005
    ..of the flavin isoalloxazine ring, and P92, the second residue in the motif that is also in close proximity to the FAD moiety, a series of rat cb(5)r variants were produced with substitutions at either P92 or Y93, respectively...
  85. ncbi Denaturation and reactivation of dimeric human glutathione reductase--an assay for folding inhibitors
    A Nordhoff
    Institute of Biochemistry II, Universitat Heidelberg, Germany
    Eur J Biochem 245:273-82. 1997
    ..glutathione reductase (GR; which catalyzes the reaction NADPH + GSSG + H+ --> 2 GSH + NADP+) is an obligatory FAD-containing homodimer of known geometry...
  86. pmc Regulation of PutA-membrane associations by flavin adenine dinucleotide reduction
    Weimin Zhang
    Department of Biochemistry, Redox Biology Center, University of Nebraska, Lincoln, Nebraska 68588, USA
    Biochemistry 43:13165-74. 2004
    ..To distinguish the roles of substrate binding and FAD reduction in the mechanism of how PutA changes from a DNA-binding protein to a membrane-bound enzyme, the kinetic ..
  87. ncbi Combined quantum mechanical and molecular mechanical simulations of one- and two-electron reduction potentials of flavin cofactor in water, medium-chain acyl-CoA dehydrogenase, and cholesterol oxidase
    Sudeep Bhattacharyya
    Department of Chemistry and Supercomputing Institute, University of Minnesota, 207 Pleasant Street SE, Smith Hall, Minneapolis, Minnesota 55455 0431, USA
    J Phys Chem A 111:5729-42. 2007
    Flavin adenine dinucleotide (FAD) is a common cofactor in redox proteins, and its reduction potentials are controlled by the protein environment. This regulation is mainly responsible for the versatile catalytic functions of flavoenzymes...
  88. ncbi Molecular properties of membrane-bound FAD-containing D-sorbitol dehydrogenase from thermotolerant Gluconobacter frateurii isolated from Thailand
    Hirohide Toyama
    Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University, Japan
    Biosci Biotechnol Biochem 69:1120-9. 2005
    ..of membrane-bound D-sorbitol dehydrogenase (SLDH) reported: PQQ-SLDH, having pyrroloquinoline quinone (PQQ), and FAD-SLDH, containing FAD and heme c as the prosthetic groups...
  89. ncbi Thermodynamic and kinetic analysis of the isolated FAD domain of rat neuronal nitric oxide synthase altered in the region of the FAD shielding residue Phe1395
    Adrian J Dunford
    Department of Biochemistry, University of Leicester, UK
    Eur J Biochem 271:2548-60. 2004
    In rat neuronal nitric oxide synthase, Phe1395 is positioned over the FAD isoalloxazine ring. This is replaced by Trp676 in human cytochrome P450 reductase, a tryptophan in related diflavin reductases (e.g...
  90. ncbi The function of presenilin-1 in amyloid beta-peptide generation and brain development
    P Saftig
    Zentrum Biochemie und Molekulare Zellbiologie, Abteilung Biochemie II, , Germany
    Eur Arch Psychiatry Clin Neurosci 249:271-9. 1999
    Several mutations in genes that cause the familial form of Alzheimer's Disease (FAD) have been identified. All mutations in the three FAD genes, i.e...
  91. ncbi Heterologous expression of an endogenous rat cytochrome b(5)/cytochrome b(5) reductase fusion protein: identification of histidines 62 and 85 as the heme axial ligands
    C Ainsley Davis
    Department of Biochemistry and Molecular Biology, University of South Florida, Tampa, Florida 33612, USA
    Arch Biochem Biophys 400:63-75. 2002
    ..for expression of an endogenous soluble fusion protein comprising a b-type cytochrome-containing domain and a FAD-containing domain has been cloned from rat liver mRNA...
  92. doi Involvement of riboflavin kinase expression in cellular sensitivity against cisplatin
    Gen Hirano
    Department of Molecular Biology, School of Medicine, University of Occupational and Environmental Health, Kitakyushu, Fukuoka 807 8555, Japan
    Int J Oncol 38:893-902. 2011
    Flavin adenine dinucleotide (FAD) is an essential coenzyme for glutathione reductase (GR) which catalyzes the reduction of oxidized glutathione to regenerate the reduced form involved in protection against oxidative stress...
  93. ncbi Cellobiose dehydrogenase from the fungi Phanerochaete chrysosporium and Humicola insolens. A flavohemoprotein from Humicola insolens contains 6-hydroxy-FAD as the dominant active cofactor
    K Igarashi
    Department of Biomaterials Sciences, School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo ku, Tokyo 113 8657, Japan
    J Biol Chem 274:3338-44. 1999
    ..The flavin cofactor from H. insolens was found to be a mixture of 60% 6-hydroxy-FAD and 40% FAD, whereas that from P. chrysosporium CDH was normal FAD...
  94. ncbi Exploring the proline-dependent conformational change in the multifunctional PutA flavoprotein by tryptophan fluorescence spectroscopy
    Weidong Zhu
    Department of Biochemistry, Redox Biology Center, University of Nebraska, Lincoln, Nebraska 68588, USA
    Biochemistry 44:12297-306. 2005
    ..the conformational change involves a flexible domain of unknown function (residues 141-262) which is nearby the FAD-binding and PRODH active sites (residues 263-610)...
  95. doi Time-dependent transcriptional profiles of genes of the hypothalamic-pituitary-gonadal axis in medaka (Oryzias latipes) exposed to fadrozole and 17beta-trenbolone
    Xiaowei Zhang
    Department of Zoology, Michigan State University, East Lansing, Michigan 48824, USA
    Environ Toxicol Chem 27:2504-11. 2008
    Both the anabolic androgen 17beta-trenbolone (TRB) and the aromatase inhibitor fadrozole (FAD) can cause decreased plasma concentrations of estrogen (E2) and reduce fecundity of fish...
  96. doi Inhibiting intramolecular electron transfer in flavin adenine dinucleotide by host-guest interaction: a fluorescence study
    Noufal Kandoth
    Radiation and Photochemistry Division, Bhabha Atomic Research Centre, Trombay, Mumbai 400 085, India
    J Phys Chem B 114:2617-26. 2010
    ..in the photophysical properties and intramolecular electron transfer behavior of the flavin adenine dinucleotide (FAD) molecule has been investigated in the presence of the macrocyclic hosts, alpha-, beta- and gamma-cyclodextrins (..
  97. pmc Studies on the mechanism of p-hydroxyphenylacetate 3-hydroxylase from Pseudomonas aeruginosa: a system composed of a small flavin reductase and a large flavin-dependent oxygenase
    Sumita Chakraborty
    Department of Biological Chemistry, University of Michigan, Ann Arbor, Michigan 48109 5606, USA
    Biochemistry 49:372-85. 2010
    ..The reductase was purified as a stable C-terminally His-tagged yellow protein containing weakly bound FAD, and the oxygenase was purified as a stable colorless N-terminally His-tagged protein...
  98. ncbi A rate-limiting conformational change of the flavin in p-hydroxybenzoate hydroxylase is necessary for ligand exchange and catalysis: studies with 8-mercapto- and 8-hydroxy-flavins
    M Ortiz Maldonado
    Department of Biological Chemistry, University of Michigan, Ann Arbor, Michigan 48109 0606, USA
    Biochemistry 40:1091-101. 2001
    The FAD of p-hydroxybenzoate hydroxylase (PHBH) is known to exist in two conformations...
  99. ncbi [Substances inhibiting amine oxidases. III. Synthesis and amine oxidase inhibition of imidazoquinoline derivatives]
    F Chimenti
    Istituto di Chimica Farmaceutica e Tossicologica, , Sapienza, Roma
    Farmaco Sci 42:513-24. 1987
    The activity of copper and FAD dependent amine oxidases was tested with some derivatives of 3H-imidazo[4,5-h]quinoline and its isomers 3H-imidazo[4,5-f]quinoline, the chemistry of which is described in the literature (1), and Ki ..
  100. doi Reduced FANCD2 influences spontaneous SCE and RAD51 foci formation in uveal melanoma and Fanconi anaemia
    P Gravells
    The Institute for Cancer Studies, Department of Oncology, Faculty of Medicine Dentistry and Health Sciences, University of Sheffield, Sheffield, UK
    Oncogene 32:5338-46. 2013
    ..Here we demonstrate that FANCD2 expression is reduced in UM and that ectopic expression of FANCD2 increased SCE...
  101. ncbi Domain engineering of the reductase component of soluble methane monooxygenase from Methylococcus capsulatus (Bath)
    Jessica L Blazyk
    Department of Chemistry, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    J Biol Chem 279:5630-40. 2004
    ..A reductase (MMOR), which contains [2Fe-2S] and FAD cofactors, facilitates electron transfer from NADH to the hydroxylase diiron active sites where dioxygen activation ..

Research Grants78

  1. Pharmacogenomics of Human P450 Oxidoreductase
    WALTER MILLER; Fiscal Year: 2009
    ..POR is a 680 amino-acid, 78 kDa protein that contains two flavins, a flavin adenine dinucleotide moiety (FAD), and a flavin mononucleotide (FMN). There is substantial genetic variation in the hepatic P450 enzymes...
  2. Pharmacogenomics of Human P450 Oxidoreductase
    Walter L Miller; Fiscal Year: 2010
    ..POR is a 680 amino-acid, 78 kDa protein that contains two flavins, a flavin adenine dinucleotide moiety (FAD), and a flavin mononucleotide (FMN). There is substantial genetic variation in the hepatic P450 enzymes...
  3. SENSORY TRANSDUCTION IN BACTERIA
    Barry Taylor; Fiscal Year: 2000
    ..The specific aims include: 1) Test the hypothesis that flavin adenine dinucleotide (FAD) in Aer senses redox changes Date Released:05/07/1997 Date Printed: 10/22/1997 in the electron transport system, ..
  4. STRUCTURE AND MECHANISM OF A FLAVOPROTEIN DEHYDROGENASE
    Jung Ja Kim; Fiscal Year: 1993
    ..of 172,000 and contains four identical subunits, each containing one equivalent of flavin adenine dinucleotide (FAD)...
  5. Defining the role of FANCD2 in homology-directed DNA Repair
    Steven Margossian; Fiscal Year: 2007
    ..A key step in this pathway is the monoubiquitination of FANCD2, resulting in its redistribution into nuclear foci...
  6. FANCD2 Monoubiquitination in DNA Damage Responses
    Paul R Andreassen; Fiscal Year: 2010
    ..A nuclear complex of at least 8 FA proteins is required for the monoubiquitination of FANCD2. Monoubiquitination of FANCD2 is required for resistance to the crosslinker mitomycin C...
  7. FANCD2 Monoubiquitination in DNA Damage Responses
    PAUL ANDREASSEN; Fiscal Year: 2009
    ..A nuclear complex of at least 8 FA proteins is required for the monoubiquitination of FANCD2. Monoubiquitination of FANCD2 is required for resistance to the crosslinker mitomycin C...
  8. A Novel Role for the Fanconi Anemia Pathway in Replication of B[a]P-Adducted DNA
    Cyrus Vaziri; Fiscal Year: 2010
    ..The Specific Aims of this proposal are: (1) To determine the molecular basis for BPDE-induced interaction between FANCD2 (the putative effector of the FA pathway) and Pol kappa...
  9. A Novel Role for the Fanconi Anemia Pathway in Replication of B[a]P-Adducted DNA
    Cyrus Vaziri; Fiscal Year: 2009
    ..The Specific Aims of this proposal are: (1) To determine the molecular basis for BPDE-induced interaction between FANCD2 (the putative effector of the FA pathway) and Pol kappa...
  10. STRUCTURAL DETERMINANTS OF FAD and FMN-REQUIRING ENZYMES
    Bettie Sue Masters; Fiscal Year: 2005
    This proposal emanates from the interests of the Principal Investigator in flavoproteins containing both FAD and FMN as prosthetic groups, extending from her early studies on NADPI-I-cytochrome P450 reductase (CYPOR) to the three nitric ..
  11. The Fanconi anemia pathway: role in DNA interstrand cross-link repair
    Johannes Walter; Fiscal Year: 2010
    ..by defects in thirteen Fanc proteins, eight of which form a ubiquitin ligase that mono-ubiquitylates the FancI-FancD2 heterodimer (the "ID" complex)...
  12. PET IMAGING & MAPPING
    Steven Potkin; Fiscal Year: 2002
    ..metabolic progression of age-related mental decline in the autosomal dominant form of familial Alzheimer disease (FAD)...
  13. Functional Analysis of the Fanconi Pathway
    Maureen Hoatlin; Fiscal Year: 2009
    ..Current models suggest that a trigger point for the FA pathway is the activation of a downstream protein, FANCD2. A set of at least eight functionally-interdependent FA core complex proteins is indispensible for the function of ..
  14. Role of Deubiquitination in Fanconi Anemia Cancer Susceptibility Pathway
    TONY TUNG HUANG; Fiscal Year: 2010
    ..Monoubiquitination of the FA protein FANCD2 appears to be critical in the repair of DNA damage because many of the proteins that are mutated in FA are ..
  15. Role of Deubiquitination in Fanconi Anemia Cancer Susceptibility Pathway
    TONY TUNG HUANG; Fiscal Year: 2010
    ..Monoubiquitination of the FA protein FANCD2 appears to be critical in the repair of DNA damage because many of the proteins that are mutated in FA are ..
  16. Regulation of the Fanconi Anemia Pathway by Ubiquitination
    ALAN D ANDREA; Fiscal Year: 2007
    ..A, B, C, D1, D2, E, F, G, L) cooperate in a common cellular pathway, leading to the monoubiquitination of FANCD2 and its interaction in chromatin with FANCD1/BRCA2...
  17. Regulation of the Fanconi Anemia Pathway by Ubiquitination
    ALAN DAVID D apos ANDREA; Fiscal Year: 2010
    ..A, B, C, D1, D2, E, F, G, L) cooperate in a common cellular pathway, leading to the monoubiquitination of FANCD2 and its interaction in chromatin with FANCD1/BRCA2...
  18. Structure/Function of Complex II Oxidoreductase
    Gary Cecchini; Fiscal Year: 2009
    ..These include a covalently-bound FAD cofactor, three distinct iron- sulfur clusters, a membrane-bound quinone, and a b heme cofactor...
  19. Structure/Function of Complex II Oxidoreductase
    Gary Cecchini; Fiscal Year: 2010
    ..These include a covalently-bound FAD cofactor, three distinct iron- sulfur clusters, a membrane-bound quinone, and a b heme cofactor...
  20. STRUCTURAL DETERMINANTS OF FAD AND FMN REQUIRING ENZYMES
    Bettie Sue Masters; Fiscal Year: 2001
    ..NADPH-cytochrome P450 reductase (CPR) is a membrane-bound flavoprotein and requires derivatives of riboflavin, both FAD and FMN, for the transfer of electrons to the various cytochromes P450 (P450s) localized in the endoplasmic ..
  21. Clinical Applications of a Novel Fanconi Anemia Assay
    Akiko Shimamura; Fiscal Year: 2007
    ..She proposes to study the clinical applications of these findings as follows: 1. Investigate the FANCD activation assay as a new functional screen for the FA pathway, 2...
  22. STRUCTURAL/FUNCTIONAL MODULARITY IN NITRIC OXIDE SYNTHAS
    Bettie Sue Masters; Fiscal Year: 1999
    ..The experimental hypothesis is that this highly complex protein, which contains FAD, FMN, and Fe- protoporphyrin IX, as prosthetic groups, and tetrahydrobiopterin and calmodulin binding sequences in ..
  23. STRUCTURE AND REGULATION OF E. COLI DNA PHOTOLYASE
    Aziz Sancar; Fiscal Year: 1990
    ..Photolyase of E. coli, which is encoded by the phr gene, has been purified to homogeneity and shown to contain an FAD cofactor...