F10

Summary

Gene Symbol: F10
Description: coagulation factor X
Alias: FXA, coagulation factor X, Stuart-Prower factor, factor X, factor Xa, prothrombinase
Species: human
Products:     F10

Top Publications

  1. Han X, Fiehler R, Broze G. Isolation of a protein Z-dependent plasma protease inhibitor. Proc Natl Acad Sci U S A. 1998;95:9250-5 pubmed
    ..The procoagulant activity of factor Xa in a one-stage plasma coagulation assay is reduced when factor Xa is first incubated with PZ...
  2. Orcutt S, Pietropaolo C, Krishnaswamy S. Extended interactions with prothrombinase enforce affinity and specificity for its macromolecular substrate. J Biol Chem. 2002;277:46191-6 pubmed
    ..of active site docking by unique substrate residues preceding the scissile bond to the function of prothrombinase. Mutagenesis of the authentic P(1)-P(3) sequence in prethrombin 2/fragment 1...
  3. Murakami M, Rios Steiner J, Weaver S, Tulinsky A, Geiger J, Arni R. Intermolecular interactions and characterization of the novel factor Xa exosite involved in macromolecular recognition and inhibition: crystal structure of human Gla-domainless factor Xa complexed with the anticoagulant protein NAPc2 from the hematop. J Mol Biol. 2007;366:602-10 pubmed
    ..blood coagulation pathway by a two step mechanism, initially interacting with the hitherto uncharacterized factor Xa exosite involved in macromolecular recognition and subsequently inhibiting factor VIIa (K(i)=8...
  4. Alba R, Bradshaw A, Parker A, Bhella D, Waddington S, Nicklin S, et al. Identification of coagulation factor (F)X binding sites on the adenovirus serotype 5 hexon: effect of mutagenesis on FX interactions and gene transfer. Blood. 2009;114:965-71 pubmed publisher
    Recent studies have demonstrated the importance of coagulation factor X (FX) in adenovirus (Ad) serotype 5-mediated liver transduction in vivo. FX binds to the adenovirus hexon hypervariable regions (HVRs)...
  5. Bradford H, Micucci J, Krishnaswamy S. Regulated cleavage of prothrombin by prothrombinase: repositioning a cleavage site reveals the unique kinetic behavior of the action of prothrombinase on its compound substrate. J Biol Chem. 2010;285:328-38 pubmed publisher
    b>Prothrombinase converts prothrombin to thrombin via cleavage at Arg(320) followed by cleavage at Arg(271)...
  6. Samama M, Martinoli J, LeFlem L, Guinet C, Plu Bureau G, Depasse F, et al. Assessment of laboratory assays to measure rivaroxaban--an oral, direct factor Xa inhibitor. Thromb Haemost. 2010;103:815-25 pubmed publisher
    ..Standard methods for HepTest and two-step prothrombinase-induced clotting time (PiCT) resulted in a paradoxical response, with low concentrations of rivaroxaban ..
  7. Barrett Y, Wang Z, Frost C, Shenker A. Clinical laboratory measurement of direct factor Xa inhibitors: anti-Xa assay is preferable to prothrombin time assay. Thromb Haemost. 2010;104:1263-71 pubmed publisher
    Apixaban and other factor Xa (FXa) inhibitors are in late-stage clinical development for prevention and treatment of thromboembolic diseases...
  8. Krishnaswamy S. Prothrombinase complex assembly. Contributions of protein-protein and protein-membrane interactions toward complex formation. J Biol Chem. 1990;265:3708-18 pubmed
    Equilibrium binding studies of prothrombinase complex formation were undertaken using phospholipid vesicles composed of phosphatidylcholine and phosphatidylserine (PCPS), factor Va, and factor Xa modified with dansyl ..
  9. Kalafatis M, Beck D. Identification of a binding site for blood coagulation factor Xa on the heavy chain of factor Va. Amino acid residues 323-331 of factor V represent an interactive site for activated factor X. Biochemistry. 2002;41:12715-28 pubmed
    ..each) spanning amino acid region 307-351 of the heavy chain of factor Va and tested them for inhibition of prothrombinase activity...

More Information

Publications107 found, 100 shown here

  1. Ahmad S, London F, Walsh P. Binding studies of the enzyme (factor IXa) with the cofactor (factor VIIIa) in the assembly of factor-X activating complex on the activated platelet surface. J Thromb Haemost. 2003;1:2348-55 pubmed
  2. Orfeo T, Brufatto N, Nesheim M, Xu H, Butenas S, Mann K. The factor V activation paradox. J Biol Chem. 2004;279:19580-91 pubmed
    The prothrombinase complex consists of the protease factor Xa, Ca2+, and factor Va assembled on an anionic membrane...
  3. Rezaie A, Kittur F. The critical role of the 185-189-loop in the factor Xa interaction with Na+ and factor Va in the prothrombinase complex. J Biol Chem. 2004;279:48262-9 pubmed
    ..Both prothrombinase and direct binding studies indicated that K186A also has an approximately 6-fold impaired affinity for factor ..
  4. Bukys M, Blum M, Kim P, Brufatto N, Nesheim M, Kalafatis M. Incorporation of factor Va into prothrombinase is required for coordinated cleavage of prothrombin by factor Xa. J Biol Chem. 2005;280:27393-401 pubmed
    ..Kinetic constants for the overall activation of prothrombin by prothrombinase assembled with saturating concentrations of recombinant mutant factor Va were calculated, prothrombin ..
  5. Kamath P, Krishnaswamy S. Fate of membrane-bound reactants and products during the activation of human prothrombin by prothrombinase. J Biol Chem. 2008;283:30164-73 pubmed publisher
    ..mediated by its N-terminal fragment 1 (F1) domain, plays an essential role in its proteolytic activation by prothrombinase. Thrombin is produced in two cleavage reactions...
  6. Scotton C, Krupiczojc M, Konigshoff M, Mercer P, Lee Y, Kaminski N, et al. Increased local expression of coagulation factor X contributes to the fibrotic response in human and murine lung injury. J Clin Invest. 2009;119:2550-63 pubmed publisher
    ..Here we report that expression of coagulation factor X (FX) is locally increased in human and murine fibrotic lung tissue, with marked immunostaining associated ..
  7. Bradshaw A, Parker A, Duffy M, Coughlan L, Van Rooijen N, Kahari V, et al. Requirements for receptor engagement during infection by adenovirus complexed with blood coagulation factor X. PLoS Pathog. 2010;6:e1001142 pubmed publisher
    Human adenoviruses from multiple species bind to coagulation factor X (FX), yet the importance of this interaction in adenovirus dissemination is unknown...
  8. Venkateswarlu D, Perera L, Darden T, Pedersen L. Structure and dynamics of zymogen human blood coagulation factor X. Biophys J. 2002;82:1190-206 pubmed
    The solution structure and dynamics of the human coagulation factor X (FX) have been investigated to understand the key structural elements in the zymogenic form that participates in the activation process...
  9. Yegneswaran S, Mesters R, Griffin J. Identification of distinct sequences in human blood coagulation factor Xa and prothrombin essential for substrate and cofactor recognition in the prothrombinase complex. J Biol Chem. 2003;278:33312-8 pubmed
    To identify amino acid sequences in factor Xa (fXa) and prothrombin (fII) that may be involved in prothrombinase complex (fXa.factor Va.fII...
  10. Bianchini E, Orcutt S, Panizzi P, Bock P, Krishnaswamy S. Ratcheting of the substrate from the zymogen to proteinase conformations directs the sequential cleavage of prothrombin by prothrombinase. Proc Natl Acad Sci U S A. 2005;102:10099-104 pubmed
    b>Prothrombinase catalyzes thrombin formation by the ordered cleavage of two peptide bonds in prothrombin...
  11. Bukys M, Kim P, Nesheim M, Kalafatis M. A control switch for prothrombinase: characterization of a hirudin-like pentapeptide from the COOH terminus of factor Va heavy chain that regulates the rate and pathway for prothrombin activation. J Biol Chem. 2006;281:39194-204 pubmed
    ..COOH terminus of the heavy chain of factor Va (Asp-Tyr-Asp-Tyr-Gln, DYDYQ) inhibits prothrombin activation by prothrombinase in a competitive manner with respect to substrate...
  12. Rios Steiner J, Murakami M, Tulinsky A, Arni R. Active and exo-site inhibition of human factor Xa: structure of des-Gla factor Xa inhibited by NAP5, a potent nematode anticoagulant protein from Ancylostoma caninum. J Mol Biol. 2007;371:774-86 pubmed
    ..One of these nematode anticoagulant proteins, NAP5, inhibits the amidolytic activity of factor Xa (fXa) with K(i)=43 pM, and is the most potent natural fXa inhibitor identified thus far...
  13. Waddington S, McVey J, Bhella D, Parker A, Barker K, Atoda H, et al. Adenovirus serotype 5 hexon mediates liver gene transfer. Cell. 2008;132:397-409 pubmed publisher
    ..Liver infection by the FX-Ad5 complex is mediated through a heparin-binding exosite in the FX serine protease domain. This study reveals an unanticipated function for hexon in mediating liver gene transfer in vivo...
  14. Kalyuzhniy O, Di Paolo N, Silvestry M, Hofherr S, Barry M, Stewart P, et al. Adenovirus serotype 5 hexon is critical for virus infection of hepatocytes in vivo. Proc Natl Acad Sci U S A. 2008;105:5483-8 pubmed publisher
    ..Here, we report that the major Ad5 capsid protein, hexon, binds human coagulation factor X (FX) with an affinity of 229 pM...
  15. Sierko E, Wojtukiewicz M, Zimnoch L, Tokajuk P, Ostrowska Cichocka K, Kisiel W. Co-localization of Protein Z, Protein Z-Dependent protease inhibitor and coagulation factor X in human colon cancer tissue: implications for coagulation regulation on tumor cells. Thromb Res. 2012;129:e112-8 pubmed publisher
    ..The Protein Z (PZ)/protein Z-dependent protease inhibitor (ZPI) complex directly inhibits factor Xa proteolytic activity...
  16. Chen T, Li F, Shu K, Liu J, Shen C, Zhang Z, et al. [Genotypic and phenotypic analysis of a case with inherited coagulation factor X deficiency]. Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2018;35:544-547 pubmed publisher
    To explore the correlation between F10 gene mutation and its phenotype in a Chinese pedigree affected with FX deficiency...
  17. Li J, Li S, Yan L, Ding T, Linhardt R, Yu Y, et al. Fucosylated chondroitin sulfate oligosaccharides exert anticoagulant activity by targeting at intrinsic tenase complex with low FXII activation: Importance of sulfation pattern and molecular size. Eur J Med Chem. 2017;139:191-200 pubmed publisher
    ..activities were measured by activated partial thromboplastin time (APTT), thrombin time (TT), thrombin and factor Xa inhibition, and activation of FXII...
  18. Olivieri O, Martinelli N, Baroni M, Branchini A, Girelli D, Friso S, et al. Factor II activity is similarly increased in patients with elevated apolipoprotein CIII and in carriers of the factor II 20210A allele. J Am Heart Assoc. 2013;2:e000440 pubmed publisher
    ..Activated factor X (FXa) generation assay was performed on plasma from subgroups of subjects with low and high levels of Apo CIII...
  19. Louw S, Saragas N, Ferrao P, Chirwa T, Jacobson B. Correlation between rivaroxaban (Xarelto) plasma activity, patient clinical variables and outcomes in a South African centre. S Afr Med J. 2016;106:1017-1020 pubmed publisher
    ..Rivaroxaban, a DOAC targeting activated coagulation factor X (FXa), is registered for the prevention and treatment of VTED in South Africa...
  20. Jin Y, Cheng X, Zheng J, Xia H, Yang L, Wang M. A novel factor X mutation Cys81 by Arg and a reported factor VII polymorphism Arg353 replaced by Gln co-occured in a patient. Blood Coagul Fibrinolysis. 2017;: pubmed publisher
    b>Coagulation factor X and factor VII (FVII) are both very important components in blood coagulation...
  21. Schöchl H, van Griensven M, Heitmeier S, Laux V, Kipman U, Roodt J, et al. Dual inhibition of thrombin and activated factor X attenuates disseminated intravascular coagulation and protects organ function in a baboon model of severe Gram-negative sepsis. Crit Care. 2017;21:51 pubmed publisher
    ..We examined whether a dual short-acting thrombin (factor II) and factor X (FX)a inhibitor (SATI) ameliorates sepsis-induced disseminated intravascular coagulation (DIC) and is organ-..
  22. Dixon Jimenez A, Brainard B, Brooks M, Nie B, Arnold R, Loper D, et al. Pharmacokinetic and pharmacodynamic evaluation of oral rivaroxaban in healthy adult cats. J Vet Emerg Crit Care (San Antonio). 2016;26:619-29 pubmed publisher
    ..based on prolongation of dilute prothrombin time, activated partial thromboplastin time (aPTT), activated Factor X (FXa) inhibition (anti-Xa activity [aXa]) and high-pressure liquid chromatography tandem mass spectrometry ..
  23. Swieringa F, Kuijpers M, Lamers M, van der Meijden P, Heemskerk J. Rate-limiting roles of the tenase complex of factors VIII and IX in platelet procoagulant activity and formation of platelet-fibrin thrombi under flow. Haematologica. 2015;100:748-56 pubmed publisher
    The importance of factor Xa generation in thrombus formation has not been studied extensively so far...
  24. Collin N, Assumpção T, Mizurini D, Gilmore D, Dutra Oliveira A, Kotsyfakis M, et al. Lufaxin, a novel factor Xa inhibitor from the salivary gland of the sand fly Lutzomyia longipalpis blocks protease-activated receptor 2 activation and inhibits inflammation and thrombosis in vivo. Arterioscler Thromb Vasc Biol. 2012;32:2185-98 pubmed publisher
    ..In addition, Lufaxin blocks prothrombinase and increases both prothrombin time and activated partial thromboplastin time...
  25. Williams F, Carter A, Hysi P, Surdulescu G, Hodgkiss D, Soranzo N, et al. Ischemic stroke is associated with the ABO locus: the EuroCLOT study. Ann Neurol. 2013;73:16-31 pubmed publisher
    ..811). ABO gene variants are associated with large-vessel and cardioembolic stroke but not small-vessel disease. This work sheds light on the different pathogenic mechanisms underpinning stroke subtype. ..
  26. Njayou F, Amougou A, Fouemene Tsayem R, Njikam Manjia J, Rudraiah S, Bradley B, et al. Antioxidant fractions of Khaya grandifoliola C.DC. and Entada africana Guill. et Perr. induce nuclear translocation of Nrf2 in HC-04 cells. Cell Stress Chaperones. 2015;20:991-1000 pubmed publisher
    ..For both plants, the methylene chloride/methanol (90/10; v/v) fraction (F10), methylene chloride/methanol (75/25; v/v) (F25), and the methanolic fraction (F100) were found to have the highest ..
  27. Nakamura T, Miyabe H, Hyodo M, Sato Y, Hayakawa Y, Harashima H. Liposomes loaded with a STING pathway ligand, cyclic di-GMP, enhance cancer immunotherapy against metastatic melanoma. J Control Release. 2015;216:149-57 pubmed publisher
    ..the activation of NK cells, resulting in a significant antitumor effect in a lung metastasis mouse model using B16-F10. This antitumor effect was dominated by NK cells...
  28. Warttinger U, Giese C, Harenberg J, Holmer E, Krämer R. A fluorescent probe assay (Heparin Red) for direct detection of heparins in human plasma. Anal Bioanal Chem. 2016;408:8241-8251 pubmed
    ..The current monitoring practice for heparin in plasma, such as the chromogenic anti-factor Xa assay, relies on heparin-triggered activation of antithrombin, an inhibitor of coagulation proteases...
  29. Xu A, Zhang L, Chen Y, Lin Z, Li R. Immunogenicity and efficacy of a rationally designed vaccine against vascular endothelial growth factor in mouse solid tumor models. Cancer Immunol Immunother. 2017;66:181-192 pubmed publisher
    ..The splenocytes from DTT-VEGF-immunized mice showed cytotoxic activity against B16-F10 cells expressing VEGF...
  30. Brown J, Jones C, Rooney L, Caughey G, Hall I. Tryptase's potent mitogenic effects in human airway smooth muscle cells are via nonproteolytic actions. Am J Physiol Lung Cell Mol Physiol. 2002;282:L197-206 pubmed
    ..in DNA synthesis far exceeded those occurring in response to coagulation cascade proteases, such as thrombin, factor Xa, or factor XII, or to other mast cell proteases, such as chymase or mastin...
  31. Weitz J, Connolly S, Patel I, Salazar D, Rohatagi S, Mendell J, et al. Randomised, parallel-group, multicentre, multinational phase 2 study comparing edoxaban, an oral factor Xa inhibitor, with warfarin for stroke prevention in patients with atrial fibrillation. Thromb Haemost. 2010;104:633-41 pubmed publisher
    ..In contrast, the edoxaban bid regimens were associated with more bleeding than warfarin. These results suggest that in this three-month study, edoxaban 30 or 60 mg qd are safe and well-tolerated. ..
  32. Corjon S, Gonzalez G, Henning P, Grichine A, Lindholm L, Boulanger P, et al. Cell entry and trafficking of human adenovirus bound to blood factor X is determined by the fiber serotype and not hexon:heparan sulfate interaction. PLoS ONE. 2011;6:e18205 pubmed publisher
    ..vectors administered intravenously accumulate in the liver as the result of their direct binding to blood coagulation factor X (FX) and subsequent interaction of the FX-HAdV5 complex with heparan sulfate proteoglycan (HSPG) at the ..
  33. Eisen A, Giugliano R, Ruff C, Nordio F, Gogia H, Awasty V, et al. Edoxaban vs warfarin in patients with nonvalvular atrial fibrillation in the US Food and Drug Administration approval population: An analysis from the Effective Anticoagulation with Factor Xa Next Generation in Atrial Fibrillation-Thrombolysis in Myo. Am Heart J. 2016;172:144-51 pubmed publisher
  34. Li J, Li S, Zhi Z, Yan L, Ye X, Ding T, et al. Depolymerization of Fucosylated Chondroitin Sulfate with a Modified Fenton-System and Anticoagulant Activity of the Resulting Fragments. Mar Drugs. 2016;14: pubmed
    ..and the slight decrease of the inhibition of factor X activity, results in a significant increase of anti-factor Xa (FXa)/anti-FIIa activity ratio...
  35. Raimondi P, Hylek E, Aronis K. Reversal Agents for Oral Antiplatelet and Anticoagulant Treatment During Bleeding Events: Current Strategies. Curr Pharm Des. 2017;23:1406-1423 pubmed publisher
    ..There is currently no available reversal agent for factor Xa inhibitors. Andexanet alpha is a factor Xa-specific inhibitor that is currently undergoing FDA review...
  36. Wang T, Du M, Ji Z, Ding C, Wang C, Men Y, et al. Recombinant protein rMBP-NAP restricts tumor progression by triggering antitumor immunity in mouse metastatic lung cancer. Can J Physiol Pharmacol. 2017;: pubmed publisher
    ..In present work, we attempt to elucidate the effect of rMBP-NAP at the local immune modulation in B16-F10-induced metastatic lung cancer...
  37. Banerjee M, Drummond D, Srivastava A, Daleke D, Lentz B. Specificity of soluble phospholipid binding sites on human factor Xa. Biochemistry. 2002;41:7751-62 pubmed
    We explore here the specificities of lipid regulatory sites on factor X(a) that affect the rate of factor X(a)-catalyzed prothrombin activation...
  38. Zuo P, Zhou Q, Zuo Z, Wang X, Chen L, Ma G. Effects of the factor Xa inhibitor, fondaparinux, on the stability of atherosclerotic lesions in apolipoprotein E-deficient mice. Circ J. 2015;79:2499-508 pubmed publisher
    ..b>Factor Xa (FXa) not only occupies a crucial position in the coagulation cascade responsible for thrombin generation, but ..
  39. Dittmeier M, Kraft P, Schuhmann M, Fluri F, Kleinschnitz C. Pretreatment with rivaroxaban attenuates stroke severity in rats by a dual antithrombotic and anti-inflammatory mechanism. Thromb Haemost. 2016;115:835-43 pubmed publisher
    ..This study examined whether prestroke anticoagulation with rivaroxaban, a novel direct factor Xa inhibitor, influences stroke severity, thrombin-mediated intracerebral thrombus formation and pro-inflammatory ..
  40. Vila Leahey A, Rogers D, Marshall J. The impact of ranitidine on monocyte responses in the context of solid tumors. Oncotarget. 2016;7:10891-904 pubmed publisher
    ..Oral ranitidine treatment did not reduce tumor growth in the B16-F10 melanoma, LLC1 lung cancer and EL4 thymoma models...
  41. Chaudhary S, Kuzynski M, Bottini M, Beniash E, Dokland T, Mobley C, et al. Phosphate induces formation of matrix vesicles during odontoblast-initiated mineralization in vitro. Matrix Biol. 2016;52-54:284-300 pubmed publisher
    ..In addition, the molecular composition of MV secreted by 17IIA11 cells and exosomes from blood and B16-F10 melanoma cell line was compared to identify the molecular characteristics distinguishing MV from other EV...
  42. Kwak J, Seok J, Suh H, Choi Y, Hong S, Kim D, et al. Antimelanogenic effects of luteolin 7-sulfate isolated from Phyllospadix iwatensis Makino. Br J Dermatol. 2016;175:501-11 pubmed publisher
    ..Furthermore, their antimelanogenic effects were examined using murine melanoma B16/F10 cells and human epidermal melanocytes (HEM)...
  43. Nogueira A, Drehmer D, Iacomini M, Sassaki G, Cipriani T. Biological and structural analyses of bovine heparin fractions of intermediate and high molecular weight. Carbohydr Polym. 2017;157:72-78 pubmed publisher
    ..Both fractions inhibited ?-thrombin and factor Xa in vitro and showed antithrombotic effect in vivo...
  44. Denies S, Leyman B, Huysmans H, Combes F, Mc Cafferty S, Cicchelero L, et al. Evaluation of a xenogeneic vascular endothelial growth factor-2 vaccine in two preclinical metastatic tumor models in mice. Cancer Immunol Immunother. 2017;66:1545-1555 pubmed publisher
    ..human vascular endothelial growth factor receptor-2 (hVEGFR-2) was evaluated in two murine tumor models, the B16-F10 melanoma and the EO771 breast carcinoma model...
  45. Che S, Park J, Stokol T. Tissue Factor-Expressing Tumor-Derived Extracellular Vesicles Activate Quiescent Endothelial Cells via Protease-Activated Receptor-1. Front Oncol. 2017;7:261 pubmed publisher
    ..We hypothesized that generation of activated factor X (FXa) by TF expressed on tumor-derived EV could activate protease-activated receptors (PARs) on non-activated ..
  46. Schuliga M, Grainge C, Westall G, Knight D. The fibrogenic actions of the coagulant and plasminogen activation systems in pulmonary fibrosis. Int J Biochem Cell Biol. 2018;97:108-117 pubmed publisher
    ..The coagulants, thrombin and factor Xa (FXa) evoke fibrogenic effects via cleavage of the N-terminus of protease-activated receptors (PARs)...
  47. Huang X, Dementiev A, Olson S, Gettins P. Basis for the specificity and activation of the serpin protein Z-dependent proteinase inhibitor (ZPI) as an inhibitor of membrane-associated factor Xa. J Biol Chem. 2010;285:20399-409 pubmed publisher
    The serpin ZPI is a protein Z (PZ)-dependent specific inhibitor of membrane-associated factor Xa (fXa) despite having an unfavorable P1 Tyr...
  48. Su X, Pang Z, Su G. [Role of hydatidiform mole-related gene F10 in the tumorigenicity of choriocarcinoma cell line JEG-3]. Nan Fang Yi Ke Da Xue Xue Bao. 2015;35:707-11 pubmed
    To explore the role of the hydatidiform mole-related gene F10 in the tumorigenicity of choriocarcinoma cell lines JEG-3 in nude mice...
  49. Xu C, Ren Y. Molecular modeling studies of [6,6,5] Tricyclic Fused Oxazolidinones as FXa inhibitors using 3D-QSAR, Topomer CoMFA, molecular docking and molecular dynamics simulations. Bioorg Med Chem Lett. 2015;25:4522-8 pubmed publisher
    Coagulation factor Xa (Factor Xa, FXa) is a particularly promising target for novel anticoagulant therapy. The first oral factor Xa inhibitor has been approved in the EU and Canada in 2008...
  50. Dogliotti A, Giugliano R. A novel approach indirectly comparing benefit-risk balance across anti-thrombotic therapies in patients with atrial fibrillation. Eur Heart J Cardiovasc Pharmacother. 2015;1:15-28 pubmed publisher
    ..Ranked by cost, the 3 factor Xa inhibitors were very similar (16-18% chance of being best)...
  51. Li N, Liu X, He X, Wang S, Cao S, Xia Z, et al. Structure and anticoagulant property of a sulfated polysaccharide isolated from the green seaweed Monostroma angicava. Carbohydr Polym. 2017;159:195-206 pubmed publisher
    ..PF2 also exhibited weak effect on antithrombin-dependent thrombin or factor Xa inhibition. The fibrin(ogen)olytic activity and thrombolytic activity of PF2 were also evaluated...
  52. Hell L, Wisgrill L, Ay C, Spittler A, Schwameis M, Jilma B, et al. Procoagulant extracellular vesicles in amniotic fluid. Transl Res. 2017;184:12-20.e1 pubmed publisher
    ..We applied a prothrombinase assay (that quantifies PS exposure on EVs), an EV-associated TF activity assay, a fibrin generation assay, a ..
  53. Gibson C, Finks S. Edoxaban: How Does the Newest Agent Fit into the DOAC Landscape?. Am J Med. 2017;130:900-906 pubmed publisher
    Edoxaban is the most recently approved factor Xa inhibitor within the class of direct oral anticoagulants (DOACs)...
  54. Sanada F, Muratsu J, Otsu R, Shimizu H, Koibuchi N, Uchida K, et al. Local Production of Activated Factor X in Atherosclerotic Plaque Induced Vascular Smooth Muscle Cell Senescence. Sci Rep. 2017;7:17172 pubmed publisher
    Our previous study demonstrated that coagulation factor Xa (FXa) induced endothelial cell senescence, resulting in inflammation and impaired angiogenesis...
  55. Xia S, Li J, Zu M, Li J, Liu J, Bai X, et al. Small size fullerenol nanoparticles inhibit thrombosis and blood coagulation through inhibiting activities of thrombin and FXa. Nanomedicine. 2018;14:929-939 pubmed publisher
    ..Inhibited activities of activated coagulation factor X (FXa) and thrombin were verified by experiments in vitro and AutoDock Vina...
  56. Dobesh P, Fanikos J. Direct Oral Anticoagulants for the Prevention of Stroke in Patients with Nonvalvular Atrial Fibrillation: Understanding Differences and Similarities. Drugs. 2015;75:1627-44 pubmed publisher
    ..oral anticoagulants (DOACs), such as the approved direct thrombin inhibitor dabigatran and the approved direct factor Xa inhibitors apixaban, rivaroxaban, and edoxaban, should be used for thromboprophylaxis in patients with ..
  57. Tutwiler V, Madeeva D, Ahn H, Andrianova I, Hayes V, Zheng X, et al. Platelet transactivation by monocytes promotes thrombosis in heparin-induced thrombocytopenia. Blood. 2016;127:464-72 pubmed publisher
    ..complexes through FcγRIIA and transactivation by monocyte-derived thrombin markedly increases Annexin V and factor Xa binding to platelets, consistent with the formation of procoagulant coated platelets...
  58. Yorkgitis B. Non-vitamin K antagonist oral anticoagulant reversal: hope is on the horizon. Am J Surg. 2016;212:160-4 pubmed publisher
    ..Andexanet alfa has completed promising phase III trials for the reversal of factor Xa inhibitors...
  59. Fordyce C, Hellkamp A, Lokhnygina Y, Lindner S, Piccini J, Becker R, et al. On-Treatment Outcomes in Patients With Worsening Renal Function With Rivaroxaban Compared With Warfarin: Insights From ROCKET AF. Circulation. 2016;134:37-47 pubmed publisher
    ..nonmajor clinically relevant bleeding) end points from the ROCKET AF trial (Rivaroxaban Once-Daily, Oral, Direct Factor Xa Inhibition Compared With Vitamin K Antagonism for Prevention of Stroke and Embolism Trial in Atrial Fibrillation ..
  60. Pozzi N, Di Cera E. Dual effect of histone H4 on prothrombin activation. J Thromb Haemost. 2016;14:1814-8 pubmed publisher
    ..H4 on the reactivity of prothrombin to its physiological activator factor (F) Xa, free or assembled in the prothrombinase complex...
  61. Spangfort M, Ipsen H, Sparholt S, Aasmul Olsen S, Larsen M, Mørtz E, et al. Characterization of purified recombinant Bet v 1 with authentic N-terminus, cloned in fusion with maltose-binding protein. Protein Expr Purif. 1996;8:365-73 pubmed
    ..White Birch) has been cloned and expressed in Escherichia coli as a fusion with maltose-binding protein and a Factor Xa proteolytic cleavage site...
  62. Millar D, Elliston L, Deex P, Krawczak M, Wacey A, Reynaud J, et al. Molecular analysis of the genotype-phenotype relationship in factor X deficiency. Hum Genet. 2000;106:249-57 pubmed
    b>Factor X deficiency is a rare haemorrhagic condition, normally inherited as an autosomal recessive trait, in which a variable clinical presentation correlates poorly with laboratory phenotype...
  63. Grundy J, Lavigne N, Hirama T, MacKenzie C, Pryzdial E. Binding of plasminogen and tissue plasminogen activator to plasmin-modulated factor X and factor Xa. Biochemistry. 2001;40:6293-302 pubmed
    ..laboratory has suggested that the fibrinolytic enzyme plasmin (Pn) inactivates coagulation factors X (FX) and Xa (FXa) in the presence of Ca(2+) and anionic phospholipid (aPL), producing fragments which bind plasminogen (Pg) and ..
  64. Qureshi S, Yang L, Yegneswaran S, Rezaie A. FRET studies with factor X mutants provide insight into the topography of the membrane-bound factor X/Xa. Biochem J. 2007;407:427-33 pubmed
    ..fluorescein dye in the active sites of Fl-FPR (fluorescein-D-Phe-Pro-Arg-chloromethane)-inhibited fXa (activated Factor Xa) and its N-terminal EGF deletion mutant (fXa-desEGF1), and the acceptor OR (octadecylrhodamine) dye incorporated ..
  65. Brinkman H, van Mourik J, Mertens K. Persistent factor VIII-dependent factor X activation on endothelial cells is independent of von Willebrand factor. Blood Coagul Fibrinolysis. 2008;19:190-6 pubmed publisher
    Endothelial cells are able to support the activation of coagulation factor X by activated factor IX in the presence of its cofactor, factor VIII...
  66. Than H, Loh J, Sum C, Ng H. Clinical utility of anti-Xa monitoring and differential sensitivity of prothrombin time assays: an illustrated case report. J Clin Pathol. 2015;68:318-9 pubmed publisher
  67. Zhang M, Li G, Zhang Y, Kang J. Quantitative analysis of antithrombin III binding site in low molecular weight heparins by exhausetive heparinases digestion and capillary electrophoresis. J Chromatogr B Analyt Technol Biomed Life Sci. 2017;1068-1069:78-83 pubmed publisher
    ..It was demonstrated that the CE method provides more precise data for assessing the anti-FXa activity than that of the biochemical assay method.
  68. Salek Ardakani S, Stuart A, Arrand J, Lyons S, Arrand J, Mackett M. High level expression and purification of the Epstein-Barr virus encoded cytokine viral interleukin 10: efficient removal of endotoxin. Cytokine. 2002;17:1-13 pubmed
    ..Viral interleukin 10 (IL-10) was released from the fusion protein by cleavage with the proteolytic enzyme factor Xa. We show that vIL-10 will bind to heparin and use this property to purify vIL-10 from factor Xa cleaved products ..
  69. Carlsson K, Freskgård P, Persson E, Carlsson U, Svensson M. Probing the interface between factor Xa and tissue factor in the quaternary complex tissue factor-factor VIIa-factor Xa-tissue factor pathway inhibitor. Eur J Biochem. 2003;270:2576-82 pubmed
    ..is inhibited by tissue factor pathway inhibitor (TFPI) in two steps: first TFPI is bound to the active site of factor Xa (FXa), and subsequently FXa-TFPI exerts feedback inhibition of TF-FVIIa...
  70. Livingston J, Sutherland M, Friedman H, Pryzdial E. Herpes simplex virus type 1-encoded glycoprotein C contributes to direct coagulation factor X-virus binding. Biochem J. 2006;393:529-35 pubmed publisher
    ..recently to initiate blood coagulation by FVIIa (activated Factor VII)-dependent proteolytic activation of FX (Factor X)...
  71. Izaguirre G, Olson S. Residues Tyr253 and Glu255 in strand 3 of beta-sheet C of antithrombin are key determinants of an exosite made accessible by heparin activation to promote rapid inhibition of factors Xa and IXa. J Biol Chem. 2006;281:13424-32 pubmed
    ..new exosites in strand 3 of beta-sheet C, which promote the reaction of the inhibitor with the target proteases, factor Xa and factor IXa...
  72. dela Cruz R, Jairajpuri M, Bock S. Disruption of a tight cluster surrounding tyrosine 131 in the native conformation of antithrombin III activates it for factor Xa inhibition. J Biol Chem. 2006;281:31668-76 pubmed
    ..Rates of heparin-independent Y131L and Y131A factor Xa inhibition were 25 and 29 times faster than for the control and Y131F, suggesting that Tyr(131) ring ..
  73. Vossen C, Hasstedt S, Demers C, Rosendaal F, Bovill E. Linkage analysis for three coagulation factors clustering on chromosome 13q34: factor VII, factor X and protein Z. J Thromb Haemost. 2007;5:1325-7 pubmed
  74. Hathcock J, Rusinova E, Vaananen H, Nemerson Y. Lipid-bound factor Xa regulates tissue factor activity. Biochemistry. 2007;46:6134-40 pubmed
    The activation of coagulation factor X by tissue factor (TF) and coagulation factor VIIa (VIIa) on a phospholipid surface is thought to be the key step in the initiation of blood coagulation...
  75. Girolami A, Scarparo P, Scandellari R, Allemand E. Congenital factor X deficiencies with a defect only or predominantly in the extrinsic or in the intrinsic system: a critical evaluation. Am J Hematol. 2008;83:668-71 pubmed publisher
    Congenital Factor X deficiency is commonly classified as type I, in which there is a concomitant decrease of activity and antigen (CRM negative), and in type II, in which activity is low but antigen is normal or near normal (CRM positive)...
  76. Mendell J, Tachibana M, Shi M, Kunitada S. Effects of food on the pharmacokinetics of edoxaban, an oral direct factor Xa inhibitor, in healthy volunteers. J Clin Pharmacol. 2011;51:687-94 pubmed publisher
    ..Both Japanese and Caucasian volunteers demonstrated a modest but clinically insignificant food effect. It was concluded that edoxaban can be administered without regard to food. ..
  77. Boileau C, Eme L, Brochier Armanet C, Janicki A, Zhang C, Latifi A. A eukaryotic-like sulfiredoxin involved in oxidative stress responses and in the reduction of the sulfinic form of 2-Cys peroxiredoxin in the cyanobacterium Anabaena PCC 7120. New Phytol. 2011;191:1108-18 pubmed publisher
    ..This is the first functional characterization of a Srx enzyme in a prokaryotic organism. ..
  78. Haynes L, Bouchard B, Tracy P, Mann K. Prothrombin activation by platelet-associated prothrombinase proceeds through the prethrombin-2 pathway via a concerted mechanism. J Biol Chem. 2012;287:38647-55 pubmed publisher
    ..The main source of ?-thrombin in vivo is the activation of prothrombin by the prothrombinase complex assembled on either an activated cell membrane or cell fragment, the most relevant of which is the ..
  79. Liang Q, Chen Q, Ding Q, Wu F, Wang X, Xi X, et al. Six novel missense mutations causing factor X deficiency and application of thrombin generation test. Thromb Res. 2013;131:554-9 pubmed publisher
    Inherited factor X (FX) deficiency is a rare hemorrhagic condition characterized by a variable clinical presentation weakly correlating with laboratory phenotype and genotype...
  80. Göbel K, Kraft P, Pankratz S, Gross C, Korsukewitz C, Kwiecien R, et al. Prothrombin and factor X are elevated in multiple sclerosis patients. Ann Neurol. 2016;80:946-951 pubmed publisher
    ..suffering from relapsing-remitting and secondary progressive MS had significantly higher prothrombin and factor X levels than healthy donors, whereas levels were unchanged in primary progressive MS and neuromyelitis optica ..
  81. Bisson A, Angoulvant D, Philippart R, Clementy N, Babuty D, Fauchier L. Non-Vitamin K Oral Anticoagulants for Stroke Prevention in Special Populations with Atrial Fibrillation. Adv Ther. 2017;34:1283-1290 pubmed publisher
    ..e., direct thrombin inhibitors or direct factor Xa inhibitors)...
  82. Dadousis C, Pegolo S, Rosa G, Bittante G, Cecchinato A. Genome-wide association and pathway-based analysis using latent variables related to milk protein composition and cheesemaking traits in dairy cattle. J Dairy Sci. 2017;100:9085-9102 pubmed publisher
    ..F7?-?-CN, F8?S2-CN, and F9?S1-CN-Ph) and 1 F was linked to the whey protein (F10?-LA); 1 F underlined the udder health status (F6Udder health)...
  83. Bouchard B, Orfeo T, Keith H, Lavoie E, Gissel M, Fung M, et al. Microparticles Formed During Storage of Red Blood Cell Units Support Thrombin Generation. J Trauma Acute Care Surg. 2017;: pubmed publisher
    ..This study was performed to assess thrombin generation via the prothrombinase complex by washed RBCs and RBC-derived microparticles as a function of RBC unit age.
  84. Reddy S, Zhou Z, Rao K, Scott J, Watzke H, High K, et al. Molecular characterization of human factor XSan Antonio. Blood. 1989;74:1486-90 pubmed
    ..all eight exons and sequences around the splice junctions, putative promoter, and polyadenylation sites of human factor X DNA from a patient with factor X deficiency. Two genetic changes in factor X have been observed in this patient...
  85. Yegneswaran S, Hackeng T, Dawson P, Griffin J. The thrombin-sensitive region of protein S mediates phospholipid-dependent interaction with factor Xa. J Biol Chem. 2008;283:33046-52 pubmed publisher
    To test the hypothesis that factor Xa (fXa) interacts with protein S, fXa was labeled active-site specifically with a dansyl (D) dye via a Glu-Gly-Arg (EGR) tether to yield DEGR-fXa(i)...
  86. Yang L, Manithody C, Rezaie A. Functional role of O-linked and N-linked glycosylation sites present on the activation peptide of factor X. J Thromb Haemost. 2009;7:1696-702 pubmed publisher
    There are two O-linked and two N-linked glycosylation sites on the activation peptide of factor X (FX) involving residues Thr-17, Thr-29, Asn-39 and Asn-49...
  87. Berny M, Munnix I, Auger J, Schols S, Cosemans J, Panizzi P, et al. Spatial distribution of factor Xa, thrombin, and fibrin(ogen) on thrombi at venous shear. PLoS ONE. 2010;5:e10415 pubmed publisher
    ..We sought to determine where activated factor X (FXa), (pro)thrombin, and fibrin(ogen) are localized in thrombi formed under venous shear...
  88. Thorup S, Husted S, Vang M. [Monitoring options and reversal agents for oral anticoagulants]. Ugeskr Laeger. 2014;176: pubmed
    The approval of the oral direct thrombin inhibitor dabigatranetexilat and the oral factor Xa inhibitors rivaroxaban and apixaban as thromboprophylaxis challenges the position of the vitamin K antagonists (VKA)...
  89. Intagliata N, Henry Z, Maitland H, Shah N, Argo C, Northup P, et al. Direct Oral Anticoagulants in Cirrhosis Patients Pose Similar Risks of Bleeding When Compared to Traditional Anticoagulation. Dig Dis Sci. 2016;61:1721-7 pubmed publisher
    ..The aim of this study was to compare rates of bleeding in cirrhosis patients treated with DOAC (factor Xa inhibitors: rivaroxaban and apixaban) to those in cirrhosis patients treated with traditional anticoagulation (..
  90. Zhang Y, Yang H, Sun W, Wang J, Zhang B, Shen Y, et al. Ethanol extract of Ilex hainanensis Merr. exhibits anti-melanoma activity by induction of G1/S cell-cycle arrest and apoptosis. Chin J Integr Med. 2018;24:47-55 pubmed publisher
    ..Caspases-3, -8, and -9 activities were detected using the colorimetric method. In addition, a B16-F10 melanoma xenograft mouse model was used to evaluate the anti-cancer activity of IME in vivo...
  91. Wu C, Chaudhary K, Na Y, Welch D, Black P, Sonabend A, et al. Quality Assessment of Stereotactic Radiosurgery of a Melanoma Brain Metastases Model Using a Mouselike Phantom and the Small Animal Radiation Research Platform. Int J Radiat Oncol Biol Phys. 2017;99:191-201 pubmed publisher
    ..C57B6 mice underwent intracranial injection of B16-F10 melanoma cells. T1-weighted postcontrast magnetic resonance imaging (MRI) was performed on day 11 after injection...
  92. Rosano G, Spoletini I, Gianni W, Vitale C. New Advances In Atrial Fibrillation Management: The Role Of Apixaban. Curr Drug Targets. 2017;: pubmed publisher
    ..Among the NOACs, apixaban - a very specific antagonist of activated factor Xa - has pharmacokinetic and pharmacodynamic properties that allow significant efficacy in AF management...