ERCC3

Summary

Gene Symbol: ERCC3
Description: ERCC excision repair 3, TFIIH core complex helicase subunit
Alias: BTF2, GTF2H, RAD25, TFIIH, TTD2, XPB, general transcription and DNA repair factor IIH helicase subunit XPB, BTF2 p89, DNA excision repair protein ERCC-3, DNA repair helicase, DNA repair protein complementing XP-B cells, TFIIH 89 kDa subunit, TFIIH basal transcription factor complex 89 kDa subunit, TFIIH basal transcription factor complex helicase XPB subunit, TFIIH p89, TFIIH subunit XPB, basic transcription factor 2 89 kDa subunit, excision repair cross-complementation group 3, excision repair cross-complementing rodent repair deficiency, complementation group 3, xeroderma pigmentosum group B-complementing protein, xeroderma pigmentosum, complementation group B
Species: human
Products:     ERCC3

Top Publications

  1. Reardon J, Ge H, Gibbs E, Sancar A, Hurwitz J, Pan Z. Isolation and characterization of two human transcription factor IIH (TFIIH)-related complexes: ERCC2/CAK and TFIIH. Proc Natl Acad Sci U S A. 1996;93:6482-7 pubmed
    ..TFIIH* consists of a subset of the TFIIH complex proteins including ERCC3 (XPB), p62, p44, p41, and p34 but is devoid of detectable levels of ERCC2 (XPD) and CAK...
  2. Weeda G, Eveno E, Donker I, Vermeulen W, Chevallier Lagente O, Taieb A, et al. A mutation in the XPB/ERCC3 DNA repair transcription gene, associated with trichothiodystrophy. Am J Hum Genet. 1997;60:320-9 pubmed
    ..Remarkably, both TTD-A and XP-D defects are associated with subunits of TFIIH, a basal transcription factor with a second function in DNA repair...
  3. Tirode F, Busso D, Coin F, Egly J. Reconstitution of the transcription factor TFIIH: assignment of functions for the three enzymatic subunits, XPB, XPD, and cdk7. Mol Cell. 1999;3:87-95 pubmed
    ..the transcription of protein-coding genes, we have dissected the role of the basal transcription/DNA repair factor TFIIH. Having succeeded in reconstituting a functionally active TFIIH from baculovirus recombinant polypeptides, we were ..
  4. Yokoi M, Masutani C, Maekawa T, Sugasawa K, Ohkuma Y, Hanaoka F. The xeroderma pigmentosum group C protein complex XPC-HR23B plays an important role in the recruitment of transcription factor IIH to damaged DNA. J Biol Chem. 2000;275:9870-5 pubmed
    ..Here we demonstrate that XPC-HR23B interacts with general transcription factor IIH (TFIIH) both in vivo and in vitro...
  5. Dubaele S, Proietti de Santis L, Bienstock R, Keriel A, Stefanini M, Van Houten B, et al. Basal transcription defect discriminates between xeroderma pigmentosum and trichothiodystrophy in XPD patients. Mol Cell. 2003;11:1635-46 pubmed
    ..We also show that TFIIH from TTD patients, but not from XP patients, exhibits a significant in vitro basal transcription defect in ..
  6. Giglia Mari G, Coin F, Ranish J, Hoogstraten D, Theil A, Wijgers N, et al. A new, tenth subunit of TFIIH is responsible for the DNA repair syndrome trichothiodystrophy group A. Nat Genet. 2004;36:714-9 pubmed
    ..TTD) results from mutations in the XPD and XPB subunits of the DNA repair and transcription factor TFIIH. In a third form of DNA repair-deficient TTD, called group A, none of the nine subunits encoding TFIIH carried ..
  7. Giglia Mari G, Miquel C, Theil A, Mari P, Hoogstraten D, Ng J, et al. Dynamic interaction of TTDA with TFIIH is stabilized by nucleotide excision repair in living cells. PLoS Biol. 2006;4:e156 pubmed
    Transcription/repair factor IIH (TFIIH) is essential for RNA polymerase II transcription and nucleotide excision repair (NER)...
  8. Fousteri M, Vermeulen W, Van Zeeland A, Mullenders L. Cockayne syndrome A and B proteins differentially regulate recruitment of chromatin remodeling and repair factors to stalled RNA polymerase II in vivo. Mol Cell. 2006;23:471-82 pubmed
    ..These results give insight into the nature and order of molecular events that take place during TCR in the context of chromosomal DNA. ..
  9. Coin F, Oksenych V, Egly J. Distinct roles for the XPB/p52 and XPD/p44 subcomplexes of TFIIH in damaged DNA opening during nucleotide excision repair. Mol Cell. 2007;26:245-56 pubmed
    Mutations in XPB, an essential subunit of the transcription/repair factor TFIIH, lead to nucleotide excision repair (NER) defects and xeroderma pigmentosum (XP)...

More Information

Publications124 found, 100 shown here

  1. Iyer N, Reagan M, Wu K, Canagarajah B, Friedberg E. Interactions involving the human RNA polymerase II transcription/nucleotide excision repair complex TFIIH, the nucleotide excision repair protein XPG, and Cockayne syndrome group B (CSB) protein. Biochemistry. 1996;35:2157-67 pubmed
    The human basal transcription factor TFIIH plays a central role in two distinct processes. TFIIH is an obligatory component of the RNA polymerase II (RNAP II) transcription initiation complex...
  2. Chakraborty A, Wakamiya M, Venkova Canova T, Pandita R, Aguilera Aguirre L, Sarker A, et al. Neil2-null Mice Accumulate Oxidized DNA Bases in the Transcriptionally Active Sequences of the Genome and Are Susceptible to Innate Inflammation. J Biol Chem. 2015;290:24636-48 pubmed publisher
    ..mouse tissue showed the association of NEIL2 with RNA polymerase II, along with Cockayne syndrome group B protein, TFIIH, and other BER proteins...
  3. Calmels N, Greff G, Obringer C, Kempf N, Gasnier C, Tarabeux J, et al. Uncommon nucleotide excision repair phenotypes revealed by targeted high-throughput sequencing. Orphanet J Rare Dis. 2016;11:26 pubmed publisher
    ..Interestingly, our results also found ERCC2(XPD), ERCC3(XPB) or ERCC5(XPG) mutations in two cases of UV-sensitive syndrome and in two cases with mixed XP/CS phenotypes...
  4. Li W, Liu W, Kakoki A, Wang R, Adebali O, Jiang Y, et al. Nucleotide excision repair capacity increases during differentiation of human embryonic carcinoma cells into neurons and muscle cells. J Biol Chem. 2019;294:5914-5922 pubmed publisher
    ..up-regulated during differentiation, but not those of replication protein A (RPA) and transcription factor IIH (TFIIH)...
  5. Dembowski J, DeLuca N. Selective recruitment of nuclear factors to productively replicating herpes simplex virus genomes. PLoS Pathog. 2015;11:e1004939 pubmed publisher
    ..RNA polymerase II, the mediator complex, TFIID, TFIIH, and several other transcriptional activators and repressors were also affinity purified with viral DNA...
  6. Yamashita T, Okura M, Ishii Osai Y, Hida T. Diagnosis of eight groups of xeroderma pigmentosum by genetic complementation using recombinant adenovirus vectors. J Dermatol. 2016;43:1167-1173 pubmed publisher
    ..fibroblasts derived from patients with XP-A, -B, -C, -D, -F or -G were infected with the adenovirus expressing XPA, XPB, XPC, XPD, XPF or XPG, respectively, and UV-C at 5-20 J/m2 was irradiated, cell viability was clearly ..
  7. Sugasawa K. Molecular mechanisms of DNA damage recognition for mammalian nucleotide excision repair. DNA Repair (Amst). 2016;44:110-117 pubmed publisher
    ..Accumulating evidence suggests that XPA and the helicase activity of transcription factor IIH (TFIIH) cooperate to verify abnormalities in DNA chemistry...
  8. Lu H, Yu D, Hansen A, Ganguly S, Liu R, Heckert A, et al. Phase-separation mechanism for C-terminal hyperphosphorylation of RNA polymerase II. Nature. 2018;558:318-323 pubmed publisher
    ..T1 droplets, and this process is enhanced upon pre-phosphorylation by CDK7 of transcription initiation factor TFIIH1-3...
  9. Oh K, Imoto K, Emmert S, Tamura D, DiGiovanna J, Kraemer K. Nucleotide excision repair proteins rapidly accumulate but fail to persist in human XP-E (DDB2 mutant) cells. Photochem Photobiol. 2011;87:729-33 pubmed publisher
    ..NER proteins (XPC, XPB, XPG, XPA and XPF) colocalized to CPD and 6-4PP positive regions immediately (<0...
  10. Fu L, Xu X, Ren R, Wu J, Zhang W, Yang J, et al. Modeling xeroderma pigmentosum associated neurological pathologies with patients-derived iPSCs. Protein Cell. 2016;7:210-21 pubmed publisher
    ..induced pluripotent stem cells (iPSCs) harboring mutations in five different XP genes including XPA, XPB, XPC, XPG, and XPV...
  11. Li S, Shi Q, Yuan L, Qin L, Wang Y, Miao Y, et al. C-Myc-dependent repression of two oncogenic miRNA clusters contributes to triptolide-induced cell death in hepatocellular carcinoma cells. J Exp Clin Cancer Res. 2018;37:51 pubmed publisher
    ..We further showed that triptolide down-regulated the expression of c-Myc through targeting ERCC3, a newly identified triptolide-binding protein...
  12. Srivastava A, Badjatia N, Lee J, Hao B, GUNZL A. An RNA polymerase II-associated TFIIF-like complex is indispensable for SL RNA gene transcription in Trypanosoma brucei. Nucleic Acids Res. 2017;: pubmed publisher
    ..form the PIC are extremely divergent orthologues of the small nuclear RNA-activating complex, TBP, TFIIA, TFIIB, TFIIH, TFIIE and Mediator...
  13. Lewis B, Burlingame A, Myers S. Human RNA Polymerase II Promoter Recruitment in Vitro Is Regulated by O-Linked N-Acetylglucosaminyltransferase (OGT). J Biol Chem. 2016;291:14056-61 pubmed publisher
    ..and 5 of the pol II C-terminal domain are O-GlcNAcylated, suggesting an overlap with the transcription factor IIH (TFIIH)-dependent serine 5 phosphorylation events during initiation and P-TEFb (positive transcriptional elongation ..
  14. Harrod A, Fulton J, Nguyen V, Periyasamy M, Ramos Garcia L, Lai C, et al. Genomic modelling of the ESR1 Y537S mutation for evaluating function and new therapeutic approaches for metastatic breast cancer. Oncogene. 2017;36:2286-2296 pubmed publisher
    ..Further, we show that the basal transcription factor TFIIH is constitutively recruited by ER-Y537S, resulting in ligand-independent phosphorylation of Serine 118 (Ser118) by ..
  15. Paolini N, Moore K, di Summa F, Fokkema I, t Hoen P, von Lindern M. Ribosome profiling uncovers selective mRNA translation associated with eIF2 phosphorylation in erythroid progenitors. PLoS ONE. 2018;13:e0193790 pubmed publisher
    ..density was increased for 147 transcripts, among which transcriptional regulators such as Atf4, Tis7/Ifrd1, Pnrc2, Gtf2h, Mbd3, JunB and Kmt2e. Translation of 337 transcripts decreased more than average, among which Dym and Csde1...
  16. Ly H, Ikegami T. Rift Valley fever virus NSs protein functions and the similarity to other bunyavirus NSs proteins. Virol J. 2016;13:118 pubmed publisher
    ..cullin 1-Skp1-Fbox E3 ligase complex, the NSs protein promotes the degradation of at least two host proteins, the TFIIH p62 and the PKR proteins...
  17. Gao W, Xiao R, Peng B, Xu H, Shen H, Huang M, et al. Arginine methylation of HSP70 regulates retinoid acid-mediated RARβ2 gene activation. Proc Natl Acad Sci U S A. 2015;112:E3327-36 pubmed publisher
    ..R469me1 was shown to mediate the interaction between HSP70 and TFIIH, which involves in RNA polymerase II phosphorylation and thus transcriptional initiation...
  18. Rasheed M, Shahzad S, Zaeem A, Afzal I, Gul A, Khalid S. Updated strategies for the management, pathogenesis and molecular genetics of different forms of ichthyosis syndromes with prominent hair abnormalities. Arch Dermatol Res. 2017;309:773-785 pubmed publisher
    ..pathogenic molecules involved in ichthyosis syndromes with prominent hair abnormalities include SPINK5, ERCC2, ERCC3, GTF2H5, MPLKIP, ST14, CLDN1 and MBTPS2...
  19. Kim N, Lannan K, Thatcher T, Pollock S, Woeller C, Phipps R. Lipoxin B4 Enhances Human Memory B Cell Antibody Production via Upregulating Cyclooxygenase-2 Expression. J Immunol. 2018;201:3343-3351 pubmed publisher
    ..are at least partially COX2-dependent as COX2 inhibitors attenuated LXB4-stimulated BLIMP1 and Xpb-1 expression as well as IgG production...
  20. He G, Yang X, Wang G, Qi J, Mao R, Wu Z, et al. Cdk7 Is Required for Activity-Dependent Neuronal Gene Expression, Long-Lasting Synaptic Plasticity and Long-Term Memory. Front Mol Neurosci. 2017;10:365 pubmed publisher
    ..Cdk7 is a subunit of transcriptional initiation factor II-H (TFIIH) and the only known Cdk-activating kinase (CAK) in metazoans...
  21. Theil A, Mandemaker I, van den Akker E, Swagemakers S, Raams A, Wüst T, et al. Trichothiodystrophy causative TFIIE? mutation affects transcription in highly differentiated tissue. Hum Mol Genet. 2017;26:4689-4698 pubmed publisher
    ..half of TTD patients are photosensitive due to inherited defects in the DNA repair and transcription factor II H (TFIIH)...
  22. Xiao M, Xiao S, Straaten T, Xue P, Zhang G, Zheng X, et al. Genetic polymorphisms in 19q13.3 genes associated with alteration of repair capacity to BPDE-DNA adducts in primary cultured lymphocytes. Mutat Res. 2016;812:39-47 pubmed publisher
    ..We genotyped for 9 single nucleotide polymorphisms (SNPs) in genes involved in NER (XPB rs4150441, XPC rs2228001, rs2279017 and XPF rs4781560), BER (XRCC1 rs25487, rs25489 and rs1799782) and genes ..
  23. Nozawa K, Schneider T, Cramer P. Core Mediator structure at 3.4 Å extends model of transcription initiation complex. Nature. 2017;545:248-251 pubmed publisher
    ..The hook is flexibly linked to Mediator by a conserved hinge and contacts the transcription initiation factor IIH (TFIIH) kinase that phosphorylates the carboxy (C)-terminal domain (CTD) of Pol II and was recently positioned on the PIC...
  24. Nakatsubo T, Nishitani S, Kikuchi Y, Iida S, Yamada K, Tanaka A, et al. Human mediator subunit MED15 promotes transcriptional activation. Drug Discov Ther. 2014;8:212-7 pubmed
    ..By immunostaining, we observed the colocalization of hMED15 with the general transcription factors TFIIE and TFIIH in the nucleus...
  25. Liu Y, Mattila J, Ventelä S, Yadav L, Zhang W, Lamichane N, et al. PWP1 Mediates Nutrient-Dependent Growth Control through Nucleolar Regulation of Ribosomal Gene Expression. Dev Cell. 2017;43:240-252.e5 pubmed publisher
    ..data further imply that dPWP1 acts synergistically with mTOR signaling to regulate the nucleolar localization of TFIIH, a known elongation factor of Pol I...
  26. Liu X. In vitro chromatin templates to study nucleotide excision repair. DNA Repair (Amst). 2015;36:68-76 pubmed publisher
    ..been utilized to follow direct removal of DNA damage; (c) six purified human DNA repair factors (RPA, XPA, XPC, TFIIH, XPG, and XPF-ERCC1) that have been used to reconstitute excision repair in vitro...
  27. Chlamydas S, Holz H, Samata M, Chelmicki T, Georgiev P, Pelechano V, et al. Functional interplay between MSL1 and CDK7 controls RNA polymerase II Ser5 phosphorylation. Nat Struct Mol Biol. 2016;23:580-9 pubmed publisher
    ..of MSL1 with CDK7, a subunit of the Cdk-activating kinase (CAK) complex of the general transcription factor TFIIH. Importantly, MSL1 depletion leads to decreased phosphorylation of Ser5 of RNA polymerase II...
  28. Yang G, Ibuki Y. Cigarette sidestream smoke delays nucleotide excision repair - inhibited accumulation of repair proteins at DNA lesions. Carcinogenesis. 2017;: pubmed publisher
    ..The recruitment of the repair molecules, TFIIH, XPA, and XPG to pyrimidine dimers was markedly inhibited by CSS...
  29. Chen S, Byrne R, Wood E, Cox M. Escherichia coli radD (yejH) gene: a novel function involved in radiation resistance and double-strand break repair. Mol Microbiol. 2015;95:754-68 pubmed publisher
    ..The yejH gene, which exhibits significant homology to the human transcription-coupled DNA repair gene XPB, is involved in the repair of double-strand DNA breaks...
  30. Nag R, Niggli S, Sousa Guimarães S, Vazquez Pianzola P, Suter B. Mms19 is a mitotic gene that permits Cdk7 to be fully active as a Cdk-activating kinase. Development. 2018;145: pubmed publisher
    ..When bound to Xpd and TFIIH, the CAK subunit Cdk7 phosphorylates transcriptional targets and not cell cycle Cdks...
  31. Hantsche M, Cramer P. Conserved RNA polymerase II initiation complex structure. Curr Opin Struct Biol. 2017;47:17-22 pubmed publisher
    ..The additional factors TFIID, TFIIH, and Mediator have been located on the periphery of the core initiation complex, providing the topology of the ..
  32. Plaschka C, Larivière L, Wenzeck L, Seizl M, Hemann M, Tegunov D, et al. Architecture of the RNA polymerase II-Mediator core initiation complex. Nature. 2015;518:376-80 pubmed publisher
    ..The Mediator 'arm' and 'hook' domains contribute to a 'cradle' that may position the CTD and TFIIH kinase to stimulate Pol II phosphorylation.
  33. Wang Q, Li M, Zhang X, Huang H, Huang J, Ke J, et al. Upregulation of CDK7 in gastric cancer cell promotes tumor cell proliferation and predicts poor prognosis. Exp Mol Pathol. 2016;100:514-21 pubmed publisher
    ..In addition, the complex is also an essential component of general transcription factor TFIIH which controls transcription via activating RNA polymerase II by serines 5 and 7 phosphorylation of the carboxyl-..
  34. Zhou M, Halanski M, Radonovich M, Kashanchi F, Peng J, Price D, et al. Tat modifies the activity of CDK9 to phosphorylate serine 5 of the RNA polymerase II carboxyl-terminal domain during human immunodeficiency virus type 1 transcription. Mol Cell Biol. 2000;20:5077-86 pubmed
    ..Our results demonstrate that cyclin-dependent kinase 7 (CDK7) (TFIIH) and CDK9 (P-TEFb) both associate with the HIV-1 preinitiation complex...
  35. Theil A, Hoeijmakers J, Vermeulen W. TTDA: big impact of a small protein. Exp Cell Res. 2014;329:61-8 pubmed publisher
    ..The multi-subunit transcription/repair factor IIH (TFIIH) is an important decision maker in NER, by opening the DNA double helix after the initial damage recognition and ..
  36. Narita T, Narita K, Takedachi A, Saijo M, Tanaka K. Regulation of Transcription Elongation by the XPG-TFIIH Complex Is Implicated in Cockayne Syndrome. Mol Cell Biol. 2015;35:3178-88 pubmed publisher
    ..XPG interacted with transcription elongation factors in concert with TFIIH, suggesting that the XPG-TFIIH complex serves as a transcription elongation factor...
  37. Warfield L, Luo J, Ranish J, Hahn S. Function of Conserved Topological Regions within the Saccharomyces cerevisiae Basal Transcription Factor TFIIH. Mol Cell Biol. 2016;36:2464-75 pubmed publisher
    b>TFIIH is a 10-subunit RNA polymerase II basal transcription factor with a dual role in DNA repair. TFIIH contains three enzymatic functions and over 30 conserved subdomains and topological regions...
  38. Kim T, Ebright R, Reinberg D. Mechanism of ATP-dependent promoter melting by transcription factor IIH. Science. 2000;288:1418-22 pubmed
    We show that transcription factor IIH ERCC3 subunit, the DNA helicase responsible for adenosine triphosphate (ATP)-dependent promoter melting during transcription initiation, does not interact with the promoter region that undergoes ..
  39. Bowden N, Ashton K, Vilain R, Avery Kiejda K, Davey R, Murray H, et al. Regulators of global genome repair do not respond to DNA damaging therapy but correlate with survival in melanoma. PLoS ONE. 2013;8:e70424 pubmed publisher
    ..In melanoma tumour tissue BRCA1 transcript expression correlated with poor survival and XPB expression correlated with solar elastosis levels...
  40. Ebmeier C, Erickson B, Allen B, Allen M, Kim H, Fong N, et al. Human TFIIH Kinase CDK7 Regulates Transcription-Associated Chromatin Modifications. Cell Rep. 2017;20:1173-1186 pubmed publisher
    ..Mass spectrometry identified factors that bound TFIIH-phosphorylated versus P-TEFb-phosphorylated CTD (versus unmodified); capping enzymes and H3K4 methyltransferase ..
  41. Du Y, Zhou J, Fan J, Shen Z, Chen X. Streamline proteomic approach for characterizing protein-protein interaction network in a RAD52 protein complex. J Proteome Res. 2009;8:2211-7 pubmed publisher
    ..In the second phase, a complex protein-protein interaction network, which may play important roles in coordinating the activity of DNA repair with that of cell division, was defined by the mammalian two-hybrid assay. ..
  42. Ting A, Low G, Gopalakrishnan K, Hande M. Telomere attrition and genomic instability in xeroderma pigmentosum type-b deficient fibroblasts under oxidative stress. J Cell Mol Med. 2010;14:403-16 pubmed publisher
    Xeroderma pigmentosum B (XPB/ERCC3/p89) is an ATP-dependent 3'-->5' directed DNA helicase involved in basal RNA transcription and the nucleotide excision repair (NER) pathway...
  43. Eyboulet F, Wydau Dematteis S, Eychenne T, Alibert O, Neil H, Boschiero C, et al. Mediator independently orchestrates multiple steps of preinitiation complex assembly in vivo. Nucleic Acids Res. 2015;43:9214-31 pubmed publisher
    ..med17 mutations differentially affected the global presence of several PIC components including Mediator, TBP, TFIIH modules and Pol II...
  44. Deschoemaeker S, Di Conza G, Lilla S, Martín Pérez R, Mennerich D, Boon L, et al. PHD1 regulates p53-mediated colorectal cancer chemoresistance. EMBO Mol Med. 2015;7:1350-65 pubmed publisher
    ..Active p53 allows nucleotide excision repair by interacting with the DNA helicase XPB, thereby protecting from chemotherapy-induced apoptosis...
  45. Li C, Golebiowski F, Onishi Y, Samara N, Sugasawa K, Yang W. Tripartite DNA Lesion Recognition and Verification by XPC, TFIIH, and XPA in Nucleotide Excision Repair. Mol Cell. 2015;59:1025-34 pubmed publisher
    Transcription factor IIH (TFIIH) is essential for both transcription and nucleotide excision repair (NER)...
  46. Drapkin R, Le Roy G, Cho H, Akoulitchev S, Reinberg D. Human cyclin-dependent kinase-activating kinase exists in three distinct complexes. Proc Natl Acad Sci U S A. 1996;93:6488-93 pubmed
    Transcription factor IIH (TFIIH) is a multisubunit complex required for transcription and for DNA nucleotide excision repair...
  47. Weeda G, Rossignol M, Fraser R, Winkler G, Vermeulen W, van T Veer L, et al. The XPB subunit of repair/transcription factor TFIIH directly interacts with SUG1, a subunit of the 26S proteasome and putative transcription factor. Nucleic Acids Res. 1997;25:2274-83 pubmed
    Mutations in the basal transcription initiation/DNA repair factor TFIIH are responsible for three human disorders: xeroderma pigmentosum (XP), cockayne syndrome (CS) and trichothiodystrophy (TTD)...
  48. Kershnar E, Wu S, Chiang C. Immunoaffinity purification and functional characterization of human transcription factor IIH and RNA polymerase II from clonal cell lines that conditionally express epitope-tagged subunits of the multiprotein complexes. J Biol Chem. 1998;273:34444-53 pubmed
    ..To facilitate the purification, we have developed an effective scheme that allows human TFIIH and pol II to be isolated from HeLa-derived cell lines that conditionally express the FLAG-tagged p62 subunit of ..
  49. Li G, Liu J, Abu Asab M, Masabumi S, Maru Y. XPB induces C1D expression to counteract UV-induced apoptosis. Mol Cancer Res. 2010;8:885-95 pubmed publisher
    ..We and others have previously shown that expression of xeroderma pigmentosum B (XPB) protein efficiently compensated the UV irradiation-sensitive phenotype of 27-1 cells, which lack functional XPB...
  50. Guo Y, Fu P, Zhu H, Reed E, Remick S, Petros W, et al. Correlations among ERCC1, XPB, UBE2I, EGF, TAL2 and ILF3 revealed by gene signatures of histological subtypes of patients with epithelial ovarian cancer. Oncol Rep. 2012;27:286-92 pubmed publisher
    ..ERCC1 and XPB expression was previously determined by RT-PCR in these specimens...
  51. Gibbons B, Brignole E, Azubel M, Murakami K, Voss N, Bushnell D, et al. Subunit architecture of general transcription factor TFIIH. Proc Natl Acad Sci U S A. 2012;109:1949-54 pubmed publisher
    Structures of complete 10-subunit yeast TFIIH and of a nested set of subcomplexes, containing 5, 6, and 7 subunits, have been determined by electron microscopy (EM) and 3D reconstruction...
  52. Bergmann E, Egly J. Trichothiodystrophy, a transcription syndrome. Trends Genet. 2001;17:279-86 pubmed
    ..These genes encode the helicase subunits of TFIIH, a DNA repair factor that is also required for transcription of class II genes...
  53. Jawhari A, Laine J, Dubaele S, Lamour V, Poterszman A, Coin F, et al. p52 Mediates XPB function within the transcription/repair factor TFIIH. J Biol Chem. 2002;277:31761-7 pubmed
    To further our understanding of the transcription/DNA repair factor TFIIH, we investigated the role of its p52 subunit in TFIIH function...
  54. Yuan X, Zhao J, Zentgraf H, Hoffmann Rohrer U, Grummt I. Multiple interactions between RNA polymerase I, TIF-IA and TAF(I) subunits regulate preinitiation complex assembly at the ribosomal gene promoter. EMBO Rep. 2002;3:1082-7 pubmed
    ..The results uncover an interphase for essential protein-protein interactions that facilitate Pol I preinitiation complex formation. ..
  55. Hilario E, Li Y, Nobumori Y, Liu X, Fan L. Structure of the C-terminal half of human XPB helicase and the impact of the disease-causing mutation XP11BE. Acta Crystallogr D Biol Crystallogr. 2013;69:237-46 pubmed publisher
    XPB is a DNA-dependent helicase and a subunit of the TFIIH complex required for both transcription and DNA repair. XPB contains four domains: an N-terminal domain, two conserved helicase domains (HD1 and HD2) and a C-terminal extension...
  56. Khlifi R, Olmedo P, Gil F, Hammami B, Hamza Chaffai A, Rebai A. Gene-environment interactions between ERCC2, ERCC3, XRCC1 and cadmium exposure in nasal polyposis disease. J Appl Genet. 2017;58:221-229 pubmed publisher
    ..To the best of our knowledge, this is the first report on the impact of combined effects of cadmium and ERCC3 7122 A>G (rs4150407), ERCC2 Lys751Gln (rs13181) and XRCC1 Arg399Gln (rs25487) genes in the susceptibility to NP ..
  57. Posternak V, Ung M, Cheng C, Cole M. MYC Mediates mRNA Cap Methylation of Canonical Wnt/β-Catenin Signaling Transcripts By Recruiting CDK7 and RNA Methyltransferase. Mol Cancer Res. 2017;15:213-224 pubmed publisher
    ..carboxy-terminal domain, mediated in part through an interaction between the TIP60 acetyltransferase complex and TFIIH. MYC enhances mRNA cap methylation above and beyond transcriptional induction. Mol Cancer Res; 15(2); 213-24...
  58. Chen D, Zhou Q. Tat activates human immunodeficiency virus type 1 transcriptional elongation independent of TFIIH kinase. Mol Cell Biol. 1999;19:2863-71 pubmed
    ..Recent reports have shown that Tat can also interact with the multisubunit transcription factor TFIIH complex and increase the phosphorylation of CTD by the Cdk-activating kinase (CAK) complex associated with the ..
  59. Reed E, Yu J, Davies A, Gannon J, Armentrout S. Clear cell tumors have higher mRNA levels of ERCC1 and XPB than other histological types of epithelial ovarian cancer. Clin Cancer Res. 2003;9:5299-305 pubmed
    The purpose of the present work was to investigate the relationship between mRNA expression of ERCC1 and XPB, two key genes in the nucleotide excision repair pathway, and clinical resistance of platinum-chemotherapy in histological ..
  60. Okuda M, Kinoshita M, Kakumu E, Sugasawa K, Nishimura Y. Structural Insight into the Mechanism of TFIIH Recognition by the Acidic String of the Nucleotide Excision Repair Factor XPC. Structure. 2015;23:1827-1837 pubmed publisher
    In global genome repair (GGR), XPC detects damaged nucleotides and recruits TFIIH complex...
  61. Franceschi S, Spugnesi L, Aretini P, Lessi F, Scarpitta R, Galli A, et al. Whole-exome analysis of a Li-Fraumeni family trio with a novel TP53 PRD mutation and anticipation profile. Carcinogenesis. 2017;38:938-943 pubmed publisher
    ..Comparing proband and father exome we detected 25 predicted deleterious variants including a nonsense mutation in ERCC3. Those inherited mutations are possible candidate modifiers linked to TP53, explaining the proband accelerated ..
  62. Okuda M, Nakazawa Y, Guo C, Ogi T, Nishimura Y. Common TFIIH recruitment mechanism in global genome and transcription-coupled repair subpathways. Nucleic Acids Res. 2017;45:13043-13055 pubmed publisher
    ..In GGR, XPC detects DNA lesions and recruits TFIIH via interaction with the pleckstrin homology (PH) domain of TFIIH subunit p62...
  63. Andersen S, Zhang A, Dominska M, Moriel Carretero M, Herrera Moyano E, Aguilera A, et al. High-Resolution Mapping of Homologous Recombination Events in rad3 Hyper-Recombination Mutants in Yeast. PLoS Genet. 2016;12:e1005938 pubmed publisher
    The Saccharomyces cerevisae RAD3 gene is the homolog of human XPD, an essential gene encoding a DNA helicase of the TFIIH complex involved in both nucleotide excision repair (NER) and transcription...
  64. Akoulitchev S, Chuikov S, Reinberg D. TFIIH is negatively regulated by cdk8-containing mediator complexes. Nature. 2000;407:102-6 pubmed
    ..regulate transcription by targeting the cdk7/cyclin H subunits of the general transcription initiation factor IIH (TFIIH)...
  65. Yee A, Nichols M, Wu L, Hall F, Kobayashi R, Xiong Y. Molecular cloning of CDK7-associated human MAT1, a cyclin-dependent kinase-activating kinase (CAK) assembly factor. Cancer Res. 1995;55:6058-62 pubmed
    ..of cyclin-dependent kinase (CDK)-activating kinase and as an essential component of the transcription factor TFIIH that is involved in transcription initiation and DNA repair...
  66. Jeang K. Tat, Tat-associated kinase, and transcription. J Biomed Sci. 1998;5:24-7 pubmed
    ..Here we review, in brief, the role of Tat-associated kinase in Tat-activated transcription. We discuss evidence that suggests involvement of TFIIH and/or P-TEFb.
  67. Botta E, Nardo T, Lehmann A, Egly J, Pedrini A, Stefanini M. Reduced level of the repair/transcription factor TFIIH in trichothiodystrophy. Hum Mol Genet. 2002;11:2919-28 pubmed
    ..excision repair (NER) as a consequence of mutations in XPD, XPB or TTDA, three genes that are all related to TFIIH, the multiprotein complex involved in NER and transcription...
  68. Hirschler Laszkiewicz I, Cavanaugh A, Mirza A, Lun M, Hu Q, Smink T, et al. Rrn3 becomes inactivated in the process of ribosomal DNA transcription. J Biol Chem. 2003;278:18953-9 pubmed
    ..Our results indicate that Rrn3 functions stoichiometrically in rDNA transcription and that its ability to associate with RNA polymerase I is lost upon transcription. ..
  69. Lloyd A, Tateishi S, Bieniasz P, Muesing M, Yamaizumi M, Mulder L. Effect of DNA repair protein Rad18 on viral infection. PLoS Pathog. 2006;2:e40 pubmed
    ..Furthermore, considering data published recently, it appears that the interactions between DNA repair components with incoming viruses, often result in inhibition of the infection rather than cooperation toward its establishment. ..
  70. Decordier I, De Bont K, De Bock K, Mateuca R, Roelants M, Ciardelli R, et al. Genetic susceptibility of newborn daughters to oxidative stress. Toxicol Lett. 2007;172:68-84 pubmed
    ..However, these conclusions might not be extrapolable to other types of DNA damage and need confirmation in a study on a larger population. ..
  71. Kainov D, Vitorino M, Cavarelli J, Poterszman A, Egly J. Structural basis for group A trichothiodystrophy. Nat Struct Mol Biol. 2008;15:980-4 pubmed
    ..TTD-A) carry mutations in the gene encoding the p8 subunit of the transcription and DNA repair factor TFIIH. Here we describe the crystal structure of a minimal complex between Tfb5, the yeast ortholog of p8, and the C-..
  72. Moslehi R, Kumar A, Mills J, AMBROGGIO X, Signore C, Dzutsev A. Phenotype-specific adverse effects of XPD mutations on human prenatal development implicate impairment of TFIIH-mediated functions in placenta. Eur J Hum Genet. 2012;20:626-31 pubmed publisher
    ..XPD mutations, placental maldevelopment and risk of pregnancy complications, possibly due to impairment of TFIIH-mediated functions in placenta...
  73. Kikuchi Y, Umemura H, Nishitani S, Iida S, Fukasawa R, Hayashi H, et al. Human mediator MED17 subunit plays essential roles in gene regulation by associating with the transcription and DNA repair machineries. Genes Cells. 2015;20:191-202 pubmed publisher
    ..assays showed that hMED17 interacted with Pol II and the general transcription factors TFIIB, TBP, TFIIE, and TFIIH. In addition, hMED17 bound to a DNA helicase subunit of TFIIH, XPB, which is essential for both transcription and ..
  74. Ma X, Zhang Y, Sun T, Yao J. Role of ERCC2 and ERCC3 gene polymorphisms in the development of osteosarcoma. Genet Mol Res. 2016;15: pubmed publisher
    We conducted a case-control study to investigate the role of common SNPs in ERCC2 (rs13181 and rs1799793) and ERCC3 (rs4150441 and rs4150506) in the development of osteosarcoma. A 1:2 matched case-control study was conducted...
  75. Scott R, Itin P, Kleijer W, Kolb K, Arlett C, Muller H. Xeroderma pigmentosum-Cockayne syndrome complex in two patients: absence of skin tumors despite severe deficiency of DNA excision repair. J Am Acad Dermatol. 1993;29:883-9 pubmed
  76. García Martínez L, Mavankal G, Neveu J, Lane W, Ivanov D, Gaynor R. Purification of a Tat-associated kinase reveals a TFIIH complex that modulates HIV-1 transcription. EMBO J. 1997;16:2836-50 pubmed
    ..This analysis indicated that proteins comprising the multi-subunit TFIIH complex, in addition to several novel factors, were associated with Tat using both in vitro and in vivo analysis...
  77. Isel C, Karn J. Direct evidence that HIV-1 Tat stimulates RNA polymerase II carboxyl-terminal domain hyperphosphorylation during transcriptional elongation. J Mol Biol. 1999;290:929-41 pubmed
    ..HIV-1 LTR, the C-terminal domain (CTD) of RNA polymerase II is rapidly phosphorylated by transcription factor IIH (TFIIH)...
  78. Zhou M, Nekhai S, Bharucha D, Kumar A, Ge H, Price D, et al. TFIIH inhibits CDK9 phosphorylation during human immunodeficiency virus type 1 transcription. J Biol Chem. 2001;276:44633-40 pubmed
    ..The presence of TFIIH in the HIV-1 preinitiation complex inhibits CDK9 phosphorylation...
  79. Silva S, Tomar M, Paulo C, Gomes B, Azevedo A, Teixeira V, et al. Breast cancer risk and common single nucleotide polymorphisms in homologous recombination DNA repair pathway genes XRCC2, XRCC3, NBS1 and RAD51. Cancer Epidemiol. 2010;34:85-92 pubmed publisher
    ..Therefore, in our study the potential protective role of the variant allele of XRCC2 (Ex3+442G>A, R188H, rs3218536), in never breast fed women, might be related with a more efficient DNA repair activity. ..
  80. Weber A, Chung H, Springer E, Heitzmann D, Warth R. The TFIIH subunit p89 (XPB) localizes to the centrosome during mitosis. Cell Oncol. 2010;32:121-30 pubmed publisher
    The general transcription factor II H (TFIIH), comprised of a core complex and an associated CAK-complex, functions in transcription, DNA repair and cell cycle control...
  81. Alekseev S, Ayadi M, Brino L, Egly J, Larsen A, Coin F. A small molecule screen identifies an inhibitor of DNA repair inducing the degradation of TFIIH and the chemosensitization of tumor cells to platinum. Chem Biol. 2014;21:398-407 pubmed publisher
    ..We found that SP promotes a rapid and reversible degradation of XPB, a subunit of transcription/repair factor TFIIH. Such degradation depends both on ubiquitin-activating enzyme and on the 26S proteasome...
  82. Dutto I, Cazzalini O, Stivala L, Prosperi E. An improved method for the detection of nucleotide excision repair factors at local UV DNA damage sites. DNA Repair (Amst). 2017;51:79-84 pubmed publisher
    ..A number of factors participating in NER, such as the TFIIH complex and the endonuclease XPG are also involved in basal processes, e.g. transcription...
  83. Rüthemann P, Balbo Pogliano C, Codilupi T, Garajova Z, Naegeli H. Chromatin remodeler CHD1 promotes XPC-to-TFIIH handover of nucleosomal UV lesions in nucleotide excision repair. EMBO J. 2017;36:3372-3386 pubmed publisher
    ..analyses further demonstrate that CHD1 facilitates substrate handover from XPC to the downstream TFIIH (transcription factor IIH)...
  84. Xing C, Zheng M. Response to Comments on Zheng et al. "Association between Promoter Methylation of Gene ERCC3 and Benzene Hematotoxicity" Int. J. Environ. Res. Public Health 2017, 14, 1393. Int J Environ Res Public Health. 2017;14: pubmed publisher
    ..We would like to thank Moshammer and Poteser for their comments [...]. ..
  85. Ma L, Weeda G, Jochemsen A, Bootsma D, Hoeijmakers J, van der Eb A. Molecular and functional analysis of the XPBC/ERCC-3 promoter: transcription activity is dependent on the integrity of an Sp1-binding site. Nucleic Acids Res. 1992;20:217-24 pubmed
    ..Band shift assays showed that this putative Sp1-binding site can interact specifically with a nuclear factor, most likely transcription factor Sp1 (or an Sp1-like factor) in vitro. ..
  86. Weeda G, Ma L, van Ham R, van der Eb A, Hoeijmakers J. Structure and expression of the human XPBC/ERCC-3 gene involved in DNA repair disorders xeroderma pigmentosum and Cockayne's syndrome. Nucleic Acids Res. 1991;19:6301-8 pubmed
    ..Southern blot analysis revealed the presence of XPBC/ERCC-3 cross-hybridizing fragments elsewhere in the genome, which may belong to a related gene. ..
  87. Weeda G, van Ham R, Vermeulen W, Bootsma D, van der Eb A, Hoeijmakers J. A presumed DNA helicase encoded by ERCC-3 is involved in the human repair disorders xeroderma pigmentosum and Cockayne's syndrome. Cell. 1990;62:777-91 pubmed
    ..motifs conserved between two superfamilies of DNA and RNA helicases, strongly suggesting that it is a DNA repair helicase. ERCC-3-deficient rodent mutants phenotypically resemble the human repair syndrome xeroderma pigmentosum (..
  88. Humbert S, van Vuuren H, Lutz Y, Hoeijmakers J, Egly J, Moncollin V. p44 and p34 subunits of the BTF2/TFIIH transcription factor have homologies with SSL1, a yeast protein involved in DNA repair. EMBO J. 1994;13:2393-8 pubmed
    ..In addition to the previously characterized p62 and p89/ERCC3 subunits, we have cloned two other subunits of BTF2, p44 and p34...
  89. Marinoni J, Roy R, Vermeulen W, Miniou P, Lutz Y, Weeda G, et al. Cloning and characterization of p52, the fifth subunit of the core of the transcription/DNA repair factor TFIIH. EMBO J. 1997;16:1093-102 pubmed
    b>TFIIH is a multiprotein factor involved in transcription and DNA repair and is implicated in DNA repair/transcription deficiency disorders such as xeroderma pigmentosum, Cockayne syndrome and trichothiodystrophy...
  90. Wood R. DNA damage recognition during nucleotide excision repair in mammalian cells. Biochimie. 1999;81:39-44 pubmed
    ..during nucleotide excision repair (NER) are the XPA protein, the heterotrimeric RPA protein, the 6 to 9-subunit TFIIH, the XPC-hHR23B complex, the XPG nuclease, and the ERCC1-XPF nuclease...
  91. Butkiewicz D, Rusin M, Harris C, Chorazy M. Identification of four single nucleotide polymorphisms in DNA repair genes: XPA and XPB (ERCC3) in Polish population. Hum Mutat. 2000;15:577-8 pubmed
    ..studies on cancer susceptibility, we screened 35 individuals for polymorphisms in coding regions of XPA and XPB genes involved in nucleotide excision repair (NER)...