Gene Symbol: EPS15
Description: epidermal growth factor receptor pathway substrate 15
Alias: AF-1P, AF1P, MLLT5, epidermal growth factor receptor substrate 15, ALL1 fused gene from chromosome 1, protein AF-1p
Species: human
Products:     EPS15

Top Publications

  1. Uezu A, Umeda K, Tsujita K, Suetsugu S, Takenawa T, Nakanishi H. Characterization of the EFC/F-BAR domain protein, FCHO2. Genes Cells. 2011;16:868-78 pubmed publisher
    ..FCHO2 localized to clathrin-coated pits at the plasma membrane and bound to Eps15, an important adaptor protein in clathrin-mediated endocytosis. FCHO2 knockdown reduced transferrin endocytosis...
  2. Teckchandani A, Mulkearns E, Randolph T, Toida N, Cooper J. The clathrin adaptor Dab2 recruits EH domain scaffold proteins to regulate integrin ?1 endocytosis. Mol Biol Cell. 2012;23:2905-16 pubmed publisher
    ..of the pit by binding cargo, clathrin, and accessory proteins, including the Eps-homology (EH) domain proteins Eps15 and intersectin. However, clathrin-mediated endocytosis of some cargoes proceeds efficiently in AP2-depleted cells...
  3. Bean A, Davanger S, Chou M, Gerhardt B, Tsujimoto S, Chang Y. Hrs-2 regulates receptor-mediated endocytosis via interactions with Eps15. J Biol Chem. 2000;275:15271-8 pubmed
    ..We now show that Hrs-2 physically interacts with Eps15, a protein required for receptor-mediated endocytosis...
  4. Parachoniak C, Park M. Distinct recruitment of Eps15 via Its coiled-coil domain is required for efficient down-regulation of the met receptor tyrosine kinase. J Biol Chem. 2009;284:8382-94 pubmed publisher
    ..Here we report that, following Met activation, the endocytic adaptor protein, Eps15, is recruited to the plasma membrane and becomes both tyrosine-phosphorylated and ubiquitinated...
  5. de Beer T, Carter R, Lobel Rice K, Sorkin A, Overduin M. Structure and Asn-Pro-Phe binding pocket of the Eps15 homology domain. Science. 1998;281:1357-60 pubmed
    b>Eps15 homology (EH) domains are eukaryotic signaling modules that recognize proteins containing Asn-Pro-Phe (NPF) sequences. The structure of the central EH domain of Eps15 has been solved by heteronuclear magnetic resonance spectroscopy...
  6. Murakami T, Felinski E, Antonetti D. Occludin phosphorylation and ubiquitination regulate tight junction trafficking and vascular endothelial growth factor-induced permeability. J Biol Chem. 2009;284:21036-46 pubmed publisher
    ..contain the ubiquitin interacting motif, including Epsin-1, epidermal growth factor receptor pathway substrate 15 (Eps15), and hepatocyte growth factor-regulated tyrosine kinase substrate (Hrs)...
  7. Kalthoff C, Groos S, Kohl R, Mahrhold S, Ungewickell E. Clint: a novel clathrin-binding ENTH-domain protein at the Golgi. Mol Biol Cell. 2002;13:4060-73 pubmed
    ..Our results suggest that Clint might participate in the formation of clathrin-coated vesicles at the level of the trans-Golgi network and remains associated with the vesicles longer than clathrin and adaptors. ..
  8. de Beer T, Hoofnagle A, Enmon J, Bowers R, Yamabhai M, Kay B, et al. Molecular mechanism of NPF recognition by EH domains. Nat Struct Biol. 2000;7:1018-22 pubmed
    b>Eps15 homology (EH) domains are protein interaction modules that recognize Asn-Pro-Phe (NPF) motifs in their biological ligands to mediate critical events during endocytosis and signal transduction...
  9. Confalonieri S, Salcini A, Puri C, Tacchetti C, Di Fiore P. Tyrosine phosphorylation of Eps15 is required for ligand-regulated, but not constitutive, endocytosis. J Cell Biol. 2000;150:905-12 pubmed
    ..b>Eps15, an endocytic protein that is tyrosine phosphorylated by EGFR, is a candidate for such a function...

More Information


  1. Fernandez Chacon R, Achiriloaie M, Janz R, Albanesi J, Sudhof T. SCAMP1 function in endocytosis. J Biol Chem. 2000;275:12752-6 pubmed
    ..Our data suggest that one of the functions of SCAMPs is to participate in endocytosis via a mechanism which may involve the recruitment of clathrin coats to the plasma membrane and the trans-Golgi network. ..
  2. Bache K, Raiborg C, Mehlum A, Stenmark H. STAM and Hrs are subunits of a multivalent ubiquitin-binding complex on early endosomes. J Biol Chem. 2003;278:12513-21 pubmed
    ..In this work, we show that STAM1 and STAM2, as well as an endocytic regulator protein, Eps15, can be co-immunoprecipitated with Hrs both from membrane and cytosolic fractions and that recombinant Hrs, STAM1/..
  3. Chen H, Fre S, Slepnev V, Capua M, Takei K, Butler M, et al. Epsin is an EH-domain-binding protein implicated in clathrin-mediated endocytosis. Nature. 1998;394:793-7 pubmed
    ..One of these factors is Eps15, a clathrin-coat-associated protein that binds the alpha-adaptin subunit of AP-2...
  4. Benmerah A, Lamaze C, Begue B, Schmid S, Dautry Varsat A, Cerf Bensussan N. AP-2/Eps15 interaction is required for receptor-mediated endocytosis. J Cell Biol. 1998;140:1055-62 pubmed
    We have previously shown that the protein Eps15 is constitutively associated with the plasma membrane adaptor complex, AP-2, suggesting its possible role in endocytosis...
  5. Barriere H, Nemes C, Lechardeur D, Khan Mohammad M, Fruh K, Lukacs G. Molecular basis of oligoubiquitin-dependent internalization of membrane proteins in Mammalian cells. Traffic. 2006;7:282-97 pubmed
    ..Functional assays demonstrate the cargo-dependent involvement of eps15/15R and epsin, UIM containing clathrin adaptors, in the endocytosis of model proteins, CD4 and the activated beta(..
  6. van Delft S, Schumacher C, Hage W, Verkleij A, van Bergen en Henegouwen P. Association and colocalization of Eps15 with adaptor protein-2 and clathrin. J Cell Biol. 1997;136:811-21 pubmed
    b>Eps15 has been identified as a substrate of the EGF receptor tyrosine kinase. In this report, we show that activation of the EGF receptor by either EGF or TGF-alpha results in phosphorylation of Eps15...
  7. Schumacher C, Knudsen B, Ohuchi T, Di Fiore P, Glassman R, Hanafusa H. The SH3 domain of Crk binds specifically to a conserved proline-rich motif in Eps15 and Eps15R. J Biol Chem. 1995;270:15341-7 pubmed
    ..We isolated Eps15, a substrate of the epidermal growth factor receptor (EGFR) tyrosine kinase, and Eps15R, a novel protein with high ..
  8. Sigismund S, Woelk T, Puri C, Maspero E, Tacchetti C, Transidico P, et al. Clathrin-independent endocytosis of ubiquitinated cargos. Proc Natl Acad Sci U S A. 2005;102:2760-5 pubmed
    ..the Ub-interacting motif, as shown through the ablation of three Ub-interacting motif-containing proteins, eps15, eps15R, and epsin...
  9. Morgan J, Prasad K, Jin S, Augustine G, Lafer E. Eps15 homology domain-NPF motif interactions regulate clathrin coat assembly during synaptic vesicle recycling. J Biol Chem. 2003;278:33583-92 pubmed
    Although genetic and biochemical studies suggest a role for Eps15 homology domain containing proteins in clathrin-mediated endocytosis, the specific functions of these proteins have been elusive...
  10. Cupers P, ter Haar E, Boll W, Kirchhausen T. Parallel dimers and anti-parallel tetramers formed by epidermal growth factor receptor pathway substrate clone 15. J Biol Chem. 1997;272:33430-4 pubmed
    The recently discovered localization of epidermal growth factor receptor pathway substrate clone 15 (Eps15) to plasma membrane clathrin-coated pits and its constitutive association with the endocytic clathrin adaptor protein complex, AP-..
  11. Catarino S, Ramalho J, Marques C, Pereira P, Girao H. Ubiquitin-mediated internalization of connexin43 is independent of the canonical endocytic tyrosine-sorting signal. Biochem J. 2011;437:255-67 pubmed publisher
    ..Furthermore, the internalization of Cx43 induced by Cx43-Ub was shown to depend on its interaction with epidermal growth factor receptor substrate 15.
  12. Polo S, Sigismund S, Faretta M, Guidi M, Capua M, Bossi G, et al. A single motif responsible for ubiquitin recognition and monoubiquitination in endocytic proteins. Nature. 2002;416:451-5 pubmed
    ..Here we report that a short amino-acid stretch at the carboxy-termini of the monoubiquitinated endocytic proteins Eps15 and eps15R is indispensable for their monoubiquitination...
  13. Klapisz E, Sorokina I, Lemeer S, Pijnenburg M, Verkleij A, van Bergen en Henegouwen P. A ubiquitin-interacting motif (UIM) is essential for Eps15 and Eps15R ubiquitination. J Biol Chem. 2002;277:30746-53 pubmed
    ..b>Eps15 and its related partner Eps15R play a key role in clathrin-mediated endocytosis of transmembrane receptors...
  14. Benmerah A, Begue B, Dautry Varsat A, Cerf Bensussan N. The ear of alpha-adaptin interacts with the COOH-terminal domain of the Eps 15 protein. J Biol Chem. 1996;271:12111-6 pubmed
    The role of Eps15 in clathrin-mediated endocytosis is supported by two observations. First, it interacts specifically and constitutively with the plasma membrane adaptor AP-2...
  15. Roxrud I, Raiborg C, Pedersen N, Stang E, Stenmark H. An endosomally localized isoform of Eps15 interacts with Hrs to mediate degradation of epidermal growth factor receptor. J Cell Biol. 2008;180:1205-18 pubmed publisher
    ..The ubiquitin-binding adaptor protein Eps15 (epidermal growth factor receptor [EGFR] pathway substrate 15) functions in endocytosis of such receptors...
  16. Wong W, Kraus M, Carlomagno F, Zelano A, Druck T, Croce C, et al. The human eps15 gene, encoding a tyrosine kinase substrate, is conserved in evolution and maps to 1p31-p32. Oncogene. 1994;9:1591-7 pubmed
    ..tyrosine kinase substrates, we have previously isolated the coding sequence for a novel putative EGFR substrate, eps15, from NIH3T3 fibroblasts...
  17. Henne W, Boucrot E, Meinecke M, Evergren E, Vallis Y, Mittal R, et al. FCHo proteins are nucleators of clathrin-mediated endocytosis. Science. 2010;328:1281-4 pubmed publisher
    ..FCHo1/2 proteins bound specifically to the plasma membrane and recruited the scaffold proteins eps15 and intersectin, which in turn engaged the adaptor complex AP2...
  18. Hawryluk M, Keyel P, Mishra S, Watkins S, Heuser J, Traub L. Epsin 1 is a polyubiquitin-selective clathrin-associated sorting protein. Traffic. 2006;7:262-81 pubmed
    ..As epsin interacts with eps15, which also contains a UIM region that binds to polyubiquitin, epsin and eps15 appear to be central components of ..
  19. Doria M, Salcini A, Colombo E, Parslow T, Pelicci P, Di Fiore P. The eps15 homology (EH) domain-based interaction between eps15 and hrb connects the molecular machinery of endocytosis to that of nucleocytosolic transport. J Cell Biol. 1999;147:1379-84 pubmed
    The Eps15 homology (EH) module is a protein-protein interaction domain that establishes a network of connections involved in various aspects of endocytosis and sorting...
  20. Rosenthal J, Chen H, Slepnev V, Pellegrini L, Salcini A, Di Fiore P, et al. The epsins define a family of proteins that interact with components of the clathrin coat and contain a new protein module. J Biol Chem. 1999;274:33959-65 pubmed
    Epsin (epsin 1) is an interacting partner for the EH domain-containing region of Eps15 and has been implicated in conjunction with Eps15 in clathrin-mediated endocytosis...
  21. Sengar A, Wang W, Bishay J, Cohen S, Egan S. The EH and SH3 domain Ese proteins regulate endocytosis by linking to dynamin and Eps15. EMBO J. 1999;18:1159-71 pubmed
    ..Ese1 is constitutively associated with Eps15 proteins to form a complex with at least 14 protein-protein interaction surfaces...
  22. Haffner C, Takei K, Chen H, Ringstad N, Hudson A, Butler M, et al. Synaptojanin 1: localization on coated endocytic intermediates in nerve terminals and interaction of its 170 kDa isoform with Eps15. FEBS Lett. 1997;419:175-80 pubmed
    ..Furthermore, we report that synaptojanin-170, an alternatively spliced isoform of synaptojanin 1, binds Eps15, a clathrin coat-associated protein...
  23. Salcini A, Confalonieri S, Doria M, Santolini E, Tassi E, Minenkova O, et al. Binding specificity and in vivo targets of the EH domain, a novel protein-protein interaction module. Genes Dev. 1997;11:2239-49 pubmed
    EH is a recently identified protein-protein interaction domain found in the signal transducers Eps15 and Eps15R and several other proteins of yeast nematode...
  24. Tebar F, Confalonieri S, Carter R, Di Fiore P, Sorkin A. Eps15 is constitutively oligomerized due to homophilic interaction of its coiled-coil region. J Biol Chem. 1997;272:15413-8 pubmed
    b>Eps15 is a member of an emerging family of proteins containing a novel protein/protein interaction domain, the EH domain, of as yet unknown function...
  25. Koles K, Messelaar E, Feiger Z, Yu C, Frank C, Rodal A. The EHD protein Past1 controls postsynaptic membrane elaboration and synaptic function. Mol Biol Cell. 2015;26:3275-88 pubmed publisher
    ..Here, we show that the conserved membrane-remodeling C-terminal Eps15 Homology Domain (EHD) protein Past1 is required for the normal assembly of the subsynaptic muscle membrane ..
  26. Wollscheid H, Biancospino M, He F, Magistrati E, Molteni E, Lupia M, et al. Diverse functions of myosin VI elucidated by an isoform-specific α-helix domain. Nat Struct Mol Biol. 2016;23:300-308 pubmed publisher
    ..Thus, the α2-linker acts like a molecular switch that assigns myosin VI to distinct endocytic (myosin VI(long)) or migratory (myosin VI(short)) functional roles. ..
  27. Snetkov X, Weisswange I, Pfanzelter J, Humphries A, Way M. NPF motifs in the vaccinia virus protein A36 recruit intersectin-1 to promote Cdc42:N-WASP-mediated viral release from infected cells. Nat Microbiol. 2016;1:16141 pubmed publisher
    ..in the C-terminus of A36 recruit AP-2 and clathrin by interacting directly with the Epsin15 homology domains of Eps15 and intersectin-1...
  28. Huang J, Qi Z, Wu F, Kotula L, Jiang S, Chen Y. Interaction of HIV-1 gp41 core with NPF motif in Epsin: implication in endocytosis of HIV. J Biol Chem. 2008;283:14994-5002 pubmed publisher
    ..These results suggest that interaction between Epsin and the gp41 core, which may be present in the target cell membrane, is probably essential for endocytosis of HIV-1, an alternative pathway of HIV-1 entry into the target cell. ..
  29. Dun W, Danilo P, Mohler P, Boyden P. Microtubular remodeling and decreased expression of Nav1.5 with enhanced EHD4 in cells from the infarcted heart. Life Sci. 2018;201:72-80 pubmed publisher
    ..and endosomal-based membrane trafficking and function are linked to tubulin, microtubular (MT) networks, and Eps15 homology domain containing proteins like EHD4...
  30. Ribeiro Rodrigues T, Catarino S, Marques C, Ferreira J, Martins Marques T, Pereira P, et al. AMSH-mediated deubiquitination of Cx43 regulates internalization and degradation of gap junctions. FASEB J. 2014;28:4629-41 pubmed publisher
    ..Overall, these data strongly suggest that AMSH-mediated deubiquitination of Cx43 protects GJs from degradation. ..
  31. Haugen L, Skjeldal F, Bergeland T, Bakke O. Endosomal binding kinetics of Eps15 and Hrs specifically regulate the degradation of RTKs. Sci Rep. 2017;7:17962 pubmed publisher
    Activation of EGF-R and PDGF-R triggers autophosphorylation and the recruitment of Eps15 and Hrs. These two endosomal proteins are important for specific receptor sorting...
  32. Carroll S, Stimpson H, Weinberg J, Toret C, Sun Y, Drubin D. Analysis of yeast endocytic site formation and maturation through a regulatory transition point. Mol Biol Cell. 2012;23:657-68 pubmed publisher
    ..Because Ede1p (homologue of Eps15) and clathrin are the early-arriving proteins most important for cargo uptake, their roles during the early stages ..
  33. Lapierre L, Manning E, Mitchell K, Caldwell C, Goldenring J. Interaction of phosphorylated Rab11-FIP2 with Eps15 regulates apical junction composition. Mol Biol Cell. 2017;28:1088-1100 pubmed publisher
    ..We identified Eps15 as an interacting partner of phospho-S227-Rab11-FIP2 (pS227-FIP2)...
  34. Hochrainer K, Racchumi G, Zhang S, Iadecola C, Anrather J. Monoubiquitination of nuclear RelA negatively regulates NF-?B activity independent of proteasomal degradation. Cell Mol Life Sci. 2012;69:2057-73 pubmed publisher
    ..These findings indicate a degradation-independent mechanism for regulating the activity of nuclear RelA by ubiquitination. ..
  35. Taylor H, Khatua A, Popik W. The innate immune factor apolipoprotein L1 restricts HIV-1 infection. J Virol. 2014;88:592-603 pubmed publisher
    ..Deciphering the precise mechanism of APOL1-mediated HIV-1 restriction may facilitate the design of unique therapeutics to target HIV-1 replication. ..
  36. Bahl K, Xie S, Spagnol G, Sorgen P, Naslavsky N, Caplan S. EHD3 Protein Is Required for Tubular Recycling Endosome Stabilization, and an Asparagine-Glutamic Acid Residue Pair within Its Eps15 Homology (EH) Domain Dictates Its Selective Binding to NPF Peptides. J Biol Chem. 2016;291:13465-78 pubmed publisher
    ..The C-terminal Eps15 homology domain (EHD)-containing proteins have been implicated in the bending and fission of TRE, thus regulating ..
  37. Kostaras E, Pedersen N, Stenmark H, Fotsis T, Murphy C. SARA and RNF11 at the crossroads of EGFR signaling and trafficking. Methods Enzymol. 2014;535:225-47 pubmed publisher
  38. Wang L, Johnson A, Hanna M, Audhya A. Eps15 membrane-binding and -bending activity acts redundantly with Fcho1 during clathrin-mediated endocytosis. Mol Biol Cell. 2016;27:2675-87 pubmed publisher
    ..Here we uncover a synthetic genetic interaction between C. elegans Fcho1 (FCHO-1) and Eps15 (EHS-1), suggesting that they function in a parallel and potentially redundant manner...
  39. Dutta D, Chakraborty S, Bandyopadhyay C, Valiya Veettil M, Ansari M, Singh V, et al. EphrinA2 regulates clathrin mediated KSHV endocytosis in fibroblast cells by coordinating integrin-associated signaling and c-Cbl directed polyubiquitination. PLoS Pathog. 2013;9:e1003510 pubmed publisher
  40. Cypher L, Bielecki T, Adepegba O, Huang L, An W, Iseka F, et al. CSF-1 receptor signalling is governed by pre-requisite EHD1 mediated receptor display on the macrophage cell surface. Cell Signal. 2016;28:1325-35 pubmed publisher
    ..However, the mechanistic details governing this process are incompletely understood. C-terminal Eps15 Homology Domain-containing (EHD) proteins have recently emerged as key regulators of receptor trafficking but have ..
  41. Pope G, Tilve S, McArdle C, Lolait S, O Carroll A. Agonist-induced internalization and desensitization of the apelin receptor. Mol Cell Endocrinol. 2016;437:108-119 pubmed publisher
    ..using dominant-negative mutant (DNM) cDNA constructs of G protein-coupled receptor kinase 2 (GRK2), ?-arrestin1, EPS15 and dynamin...
  42. De Schutter J, Guillabert A, Imbault V, Degraef C, Erneux C, Communi D, et al. SHIP2 (SH2 domain-containing inositol phosphatase 2) SH2 domain negatively controls SHIP2 monoubiquitination in response to epidermal growth factor. J Biol Chem. 2009;284:36062-76 pubmed publisher
    ..The present study thus reveals an unexpected and novel role of SHIP2 SH2 domain in the regulation of its newly identified monoubiquitination. ..
  43. Meng Q, Xing Y, Ren T, Lu H, Xi Y, Jiang Z, et al. Mammalian Eps15 homology domain 1 promotes metastasis in non-small cell lung cancer by inducing epithelial-mesenchymal transition. Oncotarget. 2017;8:22433-22442 pubmed publisher
    ..In this study, we hypothesized that Mammalian Eps15 homology domain 1 (EHD1) might be responsible for the metastatic behavior of cells in NSCLC...
  44. Dorot O, Steller H, Segal D, Horowitz M. Past1 Modulates Drosophila Eye Development. PLoS ONE. 2017;12:e0169639 pubmed publisher
    ..EHDs are a family of Eps15 Homology Domain containing proteins that consists of four mammalian homologs, one C...
  45. Chen S, Cheng M, Chen K, Horng J, Liu C, Wang S, et al. Antiviral activities of Schizonepeta tenuifolia Briq. against enterovirus 71 in vitro and in vivo. Sci Rep. 2017;7:935 pubmed publisher
    ..It was accompanied by a decline in EV71-associated hyperphosphorylation of p38 kinase and EPS15. It is plausible that STE may inhibit ROS-induced p38 kinase activation, and subsequent hnRNP A1 relocation and ..
  46. Haglund K, Schmidt M, Wong E, Guy G, Dikic I. Sprouty2 acts at the Cbl/CIN85 interface to inhibit epidermal growth factor receptor downregulation. EMBO Rep. 2005;6:635-41 pubmed
    ..Sprouty2 therefore acts as an inducible inhibitor of EGFR downregulation by targeting both the Cbl and CIN85 pathways. ..
  47. Haglund K, Shimokawa N, Szymkiewicz I, Dikic I. Cbl-directed monoubiquitination of CIN85 is involved in regulation of ligand-induced degradation of EGF receptors. Proc Natl Acad Sci U S A. 2002;99:12191-6 pubmed
    ..Thus, our data demonstrate that Cbl/Cbl-b can mediate polyubiquitination of cargo as well as monoubiquitination of CIN85 to control endosomal sorting and degradation of receptor tyrosine kinases. ..
  48. Yuan W, Lee Y, Lin S, Chang L, Tan Y, Hung C, et al. K33-Linked Polyubiquitination of Coronin 7 by Cul3-KLHL20 Ubiquitin E3 Ligase Regulates Protein Trafficking. Mol Cell. 2014;54:586-600 pubmed publisher
    ..on coronin 7 (Crn7), which facilitates Crn7 targeting to TGN through a ubiquitin-dependent interaction with Eps15. Blockage of K33-chain formation, Crn7 ubiquitination, or disruption of Crn7-Eps15 interaction impairs TGN-pool F-..
  49. Jongsma M, Berlin I, Wijdeven R, Janssen L, Janssen G, Garstka M, et al. An ER-Associated Pathway Defines Endosomal Architecture for Controlled Cargo Transport. Cell. 2016;166:152-66 pubmed publisher
    ..By drawing the endosomal system's architecture, RNF26 orchestrates endosomal maturation and trafficking of cargoes, including signaling receptors, in space and time. ..
  50. Pucharcos C, Estivill X, de la Luna S. Intersectin 2, a new multimodular protein involved in clathrin-mediated endocytosis. FEBS Lett. 2000;478:43-51 pubmed
    ..show a subcellular distribution similar to other components of the endocytic machinery and co-localize with Eps15. Moreover, their overexpression, as well as the corresponding ITSN1 protein forms, inhibits transferrin ..
  51. Stephens D, Banting G. Direct interaction of the trans-Golgi network membrane protein, TGN38, with the F-actin binding protein, neurabin. J Biol Chem. 1999;274:30080-6 pubmed
    ..These data suggest that neurabin provides a direct physical link between TGN38-containing membranes and the actin cytoskeleton. ..
  52. Krieger J, Taylor P, Gajadhar A, Guha A, Moran M, McGlade C. Identification and selected reaction monitoring (SRM) quantification of endocytosis factors associated with Numb. Mol Cell Proteomics. 2013;12:499-514 pubmed publisher
    ..In vitro binding measurements indicated exon 9-independent Numb interaction with REPS1 and Eps15 EH domains...
  53. Tong D, Liang Y, Stepanova A, Liu Y, Li X, Wang L, et al. Increased Eps15 homology domain 1 and RAB11FIP3 expression regulate breast cancer progression via promoting epithelial growth factor receptor recycling. Tumour Biol. 2017;39:1010428317691010 pubmed publisher
    Recent research indicates that the C-terminal Eps15 homology domain 1 is associated with epithelial growth factor receptor-mediated endocytosis recycling in non-small-cell lung cancer...
  54. Lewin D, Sheff D, Ooi C, Whitney J, Yamamoto E, Chicione L, et al. Cloning, expression, and localization of a novel gamma-adaptin-like molecule. FEBS Lett. 1998;435:263-8 pubmed
    ..Together, these results suggest that gamma2-adaptin plays a role in membrane transport distinct from that played by gamma-adaptin. ..
  55. Chi S, Cao H, Chen J, McNiven M. Eps15 mediates vesicle trafficking from the trans-Golgi network via an interaction with the clathrin adaptor AP-1. Mol Biol Cell. 2008;19:3564-75 pubmed publisher
    b>Eps15 (EGFR pathway substrate clone 15) is well known for its role in clathrin-coated vesicle formation at the plasma membrane through interactions with other clathrin adaptor proteins such as AP-2...
  56. N Diaye E, Hanyaloglu A, Kajihara K, Puthenveedu M, Wu P, von Zastrow M, et al. The ubiquitin-like protein PLIC-2 is a negative regulator of G protein-coupled receptor endocytosis. Mol Biol Cell. 2008;19:1252-60 pubmed publisher
    ..Identification of a negative regulator of GPCR clustering reveals a new function of ubiquitin-like proteins and highlights a cellular requirement for exquisite regulation of receptor dynamics. ..
  57. Chi S, Cao H, Wang Y, McNiven M. Recycling of the epidermal growth factor receptor is mediated by a novel form of the clathrin adaptor protein Eps15. J Biol Chem. 2011;286:35196-208 pubmed publisher
    ..Currently, the molecular machinery that regulates this sorting pathway is only partially defined. Eps15 (EGFR pathway substrate 15) is an endocytic adaptor protein that is well known to support clathrin-mediated ..
  58. Woelk T, Oldrini B, Maspero E, Confalonieri S, Cavallaro E, Di Fiore P, et al. Molecular mechanisms of coupled monoubiquitination. Nat Cell Biol. 2006;8:1246-54 pubmed
    ..Using a UIM-containing protein, eps15, as a model, we show here that coupled monoubiquitination strictly depends on the ability of the UIM to bind to ..
  59. Fisher R, Wang B, Alam S, Higginson D, Robinson H, Sundquist W, et al. Structure and ubiquitin binding of the ubiquitin-interacting motif. J Biol Chem. 2003;278:28976-84 pubmed
    ..from several proteins involved in endocytosis and vacuolar protein sorting including Hrs, Vps27p, Stam1, and Eps15 bound specifically, but with modest affinity (Kd = 0.1-1 mm), to free ubiquitin...
  60. Ko G, Paradise S, Chen H, Graham M, Vecchi M, Bianchi F, et al. Selective high-level expression of epsin 3 in gastric parietal cells, where it is localized at endocytic sites of apical canaliculi. Proc Natl Acad Sci U S A. 2010;107:21511-6 pubmed publisher
    ..We suggest that epsin 3 cooperates with other bilayer binding proteins with curvature sensing/generating properties in the specialized traffic and membrane remodeling processes typical of gastric parietal cells. ..
  61. Li H, Seth A. An RNF11: Smurf2 complex mediates ubiquitination of the AMSH protein. Oncogene. 2004;23:1801-8 pubmed
    ..Among 18 in-frame positive clones, three were found to be ZBRK1, Eps15 and AMSH (associated molecule with the SH3 domain of STAM)...
  62. Kieken F, Sharma M, Jovic M, Giridharan S, Naslavsky N, Caplan S, et al. Mechanism for the selective interaction of C-terminal Eps15 homology domain proteins with specific Asn-Pro-Phe-containing partners. J Biol Chem. 2010;285:8687-94 pubmed publisher
    Epidermal growth factor receptor tyrosine kinase substrate 15 (Eps15) homology (EH)-domain proteins can be divided into two classes: those with an N-terminal EH-domain(s), and the C-terminal Eps15 homology domain-containing proteins (..
  63. Hunt C, Kolokoltsov A, Davey R, Maury W. The Tyro3 receptor kinase Axl enhances macropinocytosis of Zaire ebolavirus. J Virol. 2011;85:334-47 pubmed publisher
    ..Our findings demonstrate for the first time that Axl enhances macropinocytosis, thereby increasing productive ZEBOV entry. ..
  64. Bertelsen V, Sak M, Breen K, Rødland M, Johannessen L, Traub L, et al. A chimeric pre-ubiquitinated EGF receptor is constitutively endocytosed in a clathrin-dependent, but kinase-independent manner. Traffic. 2011;12:507-20 pubmed publisher
    ..consisting of wt-EGFR, a C-terminal linker and four linearly connected ubiquitins was found to interact with Eps15 and epsin 1 and to be constitutively endocytosed in a clathrin-dependent manner...
  65. Savio M, Wollscheid N, Cavallaro E, Algisi V, Di Fiore P, Sigismund S, et al. USP9X Controls EGFR Fate by Deubiquitinating the Endocytic Adaptor Eps15. Curr Biol. 2016;26:173-183 pubmed publisher
    ..We identify the endocytic protein Eps15 as one of the critical substrates of USP9X, and we map the Eps15 ubiquitination sites...
  66. Mills I, Praefcke G, Vallis Y, Peter B, Olesen L, Gallop J, et al. EpsinR: an AP1/clathrin interacting protein involved in vesicle trafficking. J Cell Biol. 2003;160:213-22 pubmed
  67. Bhattacharyya S, Warfield K, Ruthel G, Bavari S, Aman M, Hope T. Ebola virus uses clathrin-mediated endocytosis as an entry pathway. Virology. 2010;401:18-28 pubmed publisher
    ..Inhibition of clathrin pathway using dominant-negative Eps15, siRNA-mediated knockdown of clathrin heavy chain, chlorpromazine and sucrose blocked EbGP pseudotyped HIV ..
  68. Cerniello F, Carretero O, Longo Carbajosa N, Cerrato B, Santos R, Grecco H, et al. MAS1 Receptor Trafficking Involves ERK1/2 Activation Through a β-Arrestin2-Dependent Pathway. Hypertension. 2017;70:982-989 pubmed publisher
    ..MAS1R internalization was blocked in the presence of shRNAcaveolin-1 and with dominant negatives for Eps15 (a protein involved in endocytosed Rs by clathrin-coated pits) and for dynamin...
  69. Rahman S, Moffitt A, Trease A, Foster K, Storck M, Band H, et al. EHD4 is a novel regulator of urinary water homeostasis. FASEB J. 2017;31:5217-5233 pubmed publisher
    The Eps15-homology domain-containing (EHD) protein family comprises 4 members that regulate endocytic recycling. Although the kidney expresses all 4 EHD proteins, their physiologic roles are largely unknown...
  70. Ducharme N, Williams J, Oztan A, Apodaca G, Lapierre L, Goldenring J. Rab11-FIP2 regulates differentiable steps in transcytosis. Am J Physiol Cell Physiol. 2007;293:C1059-72 pubmed
    ..The results indicate that Rab11-FIP2 regulates trafficking at multiple points within the apical recycling system of polarized cells. ..
  71. Uezu A, Horiuchi A, Kanda K, Kikuchi N, Umeda K, Tsujita K, et al. SGIP1alpha is an endocytic protein that directly interacts with phospholipids and Eps15. J Biol Chem. 2007;282:26481-9 pubmed
    ..SGIP1alpha furthermore bound to Eps15, an important adaptor protein of clathrin-mediated endocytic machinery...
  72. Poupon V, Begue B, Gagnon J, Dautry Varsat A, Cerf Bensussan N, Benmerah A. Molecular cloning and characterization of MT-ACT48, a novel mitochondrial acyl-CoA thioesterase. J Biol Chem. 1999;274:19188-94 pubmed
    While characterizing Eps15 partners, we identified a 48-kDa polypeptide (p48) which was precipitated by Eps15-derived glutathione S-transferase fusion proteins...
  73. Ramasamy A, Curjuric I, Coin L, Kumar A, McArdle W, Imboden M, et al. A genome-wide meta-analysis of genetic variants associated with allergic rhinitis and grass sensitization and their interaction with birth order. J Allergy Clin Immunol. 2011;128:996-1005 pubmed publisher
    ..This relatively large meta-analysis of GWASs identified few loci associated with AR and grass sensitization. No birth order interaction was identified in the current analyses. ..
  74. Baillat G, Gaillard S, Castets F, Monneron A. Interactions of phocein with nucleoside-diphosphate kinase, Eps15, and Dynamin I. J Biol Chem. 2002;277:18961-6 pubmed
    ..a yeast two-hybrid screen, we identified two novel interacting proteins, nucleoside-diphosphate kinase (NDPK) and Eps15. Immunoprecipitation and pull-down experiments involving native and/or recombinant phocein and, respectively, NDPK ..
  75. Oved S, Mosesson Y, Zwang Y, Santonico E, Shtiegman K, Marmor M, et al. Conjugation to Nedd8 instigates ubiquitylation and down-regulation of activated receptor tyrosine kinases. J Biol Chem. 2006;281:21640-51 pubmed
    ..We discuss the emerging versatility of the concerted action of ubiquitylation and neddylation in the process that desensitizes growth factor-activated receptor tyrosine kinases. ..
  76. Husain M, Moss B. Role of receptor-mediated endocytosis in the formation of vaccinia virus extracellular enveloped particles. J Virol. 2005;79:4080-9 pubmed
    ..We used EH21, a dominant-negative form of Eps15 that is an essential component of clathrin-coated pits, to investigate the extent and importance of endocytosis of ..
  77. Kotecha R, Ford J, Beesley A, Anderson D, Cole C, Kees U. Molecular characterization of identical, novel MLL-EPS15 translocation and individual genomic copy number alterations in monozygotic infant twins with acute lymphoblastic leukemia. Haematologica. 2012;97:1447-50 pubmed publisher