EMP2

Summary

Gene Symbol: EMP2
Description: epithelial membrane protein 2
Alias: XMP, EMP-2
Species: human

Top Publications

  1. pmc Steroid hormone regulation of EMP2 expression and localization in the endometrium
    Madhuri Wadehra
    Department of Pathology and Laboratory Medicine, David Geffen School of Medicine at UCLA, Los Angeles, California 90095, USA
    Reprod Biol Endocrinol 6:15. 2008
  2. ncbi Epithelial membrane protein-2 is expressed in discrete anatomical regions of the eye
    Madhuri Wadehra
    Department of Pathology and Laboratory Medicine, Jonsson Comprehensive Cancer Center, Los Angeles, CA 90095, USA
    Exp Mol Pathol 74:106-12. 2003
  3. ncbi Epithelial membrane protein-2 regulates surface expression of alphavbeta3 integrin in the endometrium
    Madhuri Wadehra
    Department of Pathology and Laboratory, University of California, Los Angeles, CA 90095, USA
    Dev Biol 287:336-45. 2005
  4. ncbi Epithelial membrane protein 2 modulates infectivity of Chlamydia muridarum (MoPn)
    Kaori Shimazaki
    Molecular Biology Institute, Los Angeles, CA 90095, USA
    Microbes Infect 9:1003-10. 2007
  5. ncbi Xanthosine and xanthine. Substrate properties with purine nucleoside phosphorylases, and relevance to other enzyme systems
    Gerasim Stoychev
    Department of Biophysics, Institute of Experimental Physics, University of Warsaw, Poland
    Eur J Biochem 269:4048-57. 2002
  6. ncbi Tritrichomonas foetus: a strategy for structure-based inhibitor design of a protozoan inosine-5'-monophosphate dehydrogenase
    H Luecke
    Department of Molecular Biology and Biochemistry, University of California, Irvine 92697, USA
    Exp Parasitol 87:203-11. 1997
  7. ncbi Evidence for the involvement of cytosolic 5'-nucleotidase (cN-II) in the synthesis of guanine nucleotides from xanthosine
    Catia Barsotti
    Department of Physiology and Biochemistry, University of Pisa, Via San Zeno 51, 56127 Pisa, Italy
    J Biol Chem 280:13465-9. 2005
  8. ncbi IMP dehydrogenase: mechanism of action and inhibition
    L Hedstrom
    MS 009, Department of Biochemistry, Brandeis University, Waltham, MA 02454, USA
    Curr Med Chem 6:545-60. 1999
  9. pmc Empty pericarp2 encodes a negative regulator of the heat shock response and is required for maize embryogenesis
    Suneng Fu
    Department of Plant Biology, University of Georgia, Athens, Georgia 30602, USA
    Plant Cell 14:3119-32. 2002
  10. ncbi Asp338 controls hydride transfer in Escherichia coli IMP dehydrogenase
    K M Kerr
    Departments of Biochemistry and Biology, Brandeis University, Waltham, Massachusetts 02454, USA
    Biochemistry 39:9804-10. 2000

Research Grants

  1. INOSINE MONOPHOSPHATE DEHYDROGENASE
    Lizbeth Hedstrom; Fiscal Year: 2001
  2. IMP Dehydrogenase
    LIZBETH K HEDSTROM; Fiscal Year: 2010
  3. IMP Dehydrogenase
    Lizbeth Hedstrom; Fiscal Year: 2007
  4. IMP Dehydrogenase
    Lizbeth Hedstrom; Fiscal Year: 2006
  5. Novel Therapies to Prevent Blindness Caused by Proliferative Vitreoretinopathy
    David G Telander; Fiscal Year: 2010
  6. Abnormal EMPS Expression Affects Pregnancy Outcome
    Jonathan Braun; Fiscal Year: 2006
  7. ENZYMATIC MECHANISMS OF SULFUR NUCLEOSIDE METABOLISM
    GEORGE MARKHAM; Fiscal Year: 2007
  8. IMP Dehydrogenase and the Hydra of Cancer Chemotherapy
    GEORGE MARKHAM; Fiscal Year: 2007
  9. Structure-Function Studies of the Proton-Gated Urea Channel from H. Pylori
    Hartmut Luecke; Fiscal Year: 2009
  10. Structure-Function Studies of the Proton-Gated Urea Channel from H. Pylori
    Hartmut Luecke; Fiscal Year: 2010

Scientific Experts

  • H Sigel
  • Michal Cesnek
  • P Glander
  • M A Fleming
  • Michael H Ramsey
  • Madhuri Wadehra
  • W Timothy J White
  • Zhi Tao Duan
  • M M Binns
  • W Markland
  • A G Medentsev
  • H Luecke
  • Bjarne Hove-Jensen
  • Eric Mick
  • Myriam Bodescot
  • GEORGE MARKHAM
  • Yasushi Noguchi
  • David G Telander
  • Lizbeth Hedstrom
  • Jonathan Braun
  • SHERRY QUEENER
  • FRANK WHITBY
  • Carmen Williams
  • Lynn K Gordon
  • Kaori Shimazaki
  • Shawn A Morales
  • Lee Goodglick
  • JOSEPH GOTS
  • Maoyong Fu
  • OWEN MOE
  • Honggao Yan
  • Lynn Gordon
  • LIZBETH K HEDSTROM
  • Sergey Mareninov
  • Hemalatha Balaram
  • Yollete V Guillen Schlippe
  • L Hedstrom
  • Ashley Forbes
  • Lu Gan
  • L K Gordon
  • Justin C Oliver
  • Matthias Christgen
  • Veeraraghavan Usha
  • Bo Pang
  • Yi Hsien Chen
  • M Khan
  • Spriha Gogia
  • Xin E Sun
  • Audrey L Fulwiler
  • Javaid Y Bhat
  • Gregory C Patton
  • Jae Hyun Park
  • Omar Habeeb
  • Shintaro Maruoka
  • Robert A Soslow
  • Jan M Boitz
  • Armando Jardim
  • Shawn Morales
  • Catherine P Coulam
  • Anna M Wu
  • Kathleen A Kelly
  • David B Langley
  • Ewa Poduch
  • Javaid Yousuf Bhat
  • Ann M Chan
  • Fredrick Dobie
  • K M Kerr
  • Chirag G Patel
  • Philipe N Khalil
  • Jessica L Abbott
  • Hong Long
  • Emil F Pai
  • Gabrielle Barnby
  • Catia Barsotti
  • Paul D Taylor
  • I Ben-Porath
  • Salah S Massoud
  • Dennis W Wolan
  • Mohammad R Seyedsayamdost
  • V Taylor
  • Glen L Prosise
  • Jarosław Poznański
  • Kouichi Mizuno
  • S Vos
  • Leonarda Ianzano
  • David Shugar
  • Borys Kierdaszuk
  • Gregory A Petsko
  • Valerie A Street
  • Suneng Fu

Detail Information

Publications104 found, 100 shown here

  1. pmc Steroid hormone regulation of EMP2 expression and localization in the endometrium
    Madhuri Wadehra
    Department of Pathology and Laboratory Medicine, David Geffen School of Medicine at UCLA, Los Angeles, California 90095, USA
    Reprod Biol Endocrinol 6:15. 2008
    The tetraspan protein epithelial membrane protein-2 (EMP2), which mediates surface display of diverse proteins, is required for endometrial competence in blastocyst implantation, and is uniquely correlated with poor survival from ..
  2. ncbi Epithelial membrane protein-2 is expressed in discrete anatomical regions of the eye
    Madhuri Wadehra
    Department of Pathology and Laboratory Medicine, Jonsson Comprehensive Cancer Center, Los Angeles, CA 90095, USA
    Exp Mol Pathol 74:106-12. 2003
    Epithelial membrane protein-2 (EMP2) is a member of the four transmembrane superfamily (TM4SF) and is thought to mediate trafficking of diverse proteins such as alpha6beta1 integrin and MHC class I to lipid raft microdomains...
  3. ncbi Epithelial membrane protein-2 regulates surface expression of alphavbeta3 integrin in the endometrium
    Madhuri Wadehra
    Department of Pathology and Laboratory, University of California, Los Angeles, CA 90095, USA
    Dev Biol 287:336-45. 2005
    The four-transmembrane protein epithelial membrane protein-2 (EMP2) was recently identified as an endometrial protein necessary for blastocyst implantation, but the mechanism of this role is uncertain...
  4. ncbi Epithelial membrane protein 2 modulates infectivity of Chlamydia muridarum (MoPn)
    Kaori Shimazaki
    Molecular Biology Institute, Los Angeles, CA 90095, USA
    Microbes Infect 9:1003-10. 2007
    ..Epithelial membrane protein-2 (EMP2), a 4-transmembrane protein, is highly expressed in epithelial cells in sites of chlamydial infections...
  5. ncbi Xanthosine and xanthine. Substrate properties with purine nucleoside phosphorylases, and relevance to other enzyme systems
    Gerasim Stoychev
    Department of Biophysics, Institute of Experimental Physics, University of Warsaw, Poland
    Eur J Biochem 269:4048-57. 2002
    ..Attention is directed to the fact that the structural properties of the neutral and ionic forms of XMP, Xao and Xan are also of key importance in many other enzyme systems, such as IMP dehydrogenase, some nucleic acid ..
  6. ncbi Tritrichomonas foetus: a strategy for structure-based inhibitor design of a protozoan inosine-5'-monophosphate dehydrogenase
    H Luecke
    Department of Molecular Biology and Biochemistry, University of California, Irvine 92697, USA
    Exp Parasitol 87:203-11. 1997
    ..The enzyme catalyzes the NAD-dependent oxidation of inosine monophosphate (IMP) to xanthosine monophosphate (XMP), the committed step in guanosine monophosphate (GMP) biosynthesis...
  7. ncbi Evidence for the involvement of cytosolic 5'-nucleotidase (cN-II) in the synthesis of guanine nucleotides from xanthosine
    Catia Barsotti
    Department of Physiology and Biochemistry, University of Pisa, Via San Zeno 51, 56127 Pisa, Italy
    J Biol Chem 280:13465-9. 2005
    ..In rat brain extracts and in intact LoVo cells, xanthosine is salvaged to XMP via the phosphotransferase activity of cytosolic 5'-nucleotidase...
  8. ncbi IMP dehydrogenase: mechanism of action and inhibition
    L Hedstrom
    MS 009, Department of Biochemistry, Brandeis University, Waltham, MA 02454, USA
    Curr Med Chem 6:545-60. 1999
    Inosine monophosphate dehydrogenase (IMPDH) catalyzes the conversion of IMP to XMP with the concomitant reduction of NAD to NADH. This reaction is the rate-limiting step in guanine nucleotide biosynthesis...
  9. pmc Empty pericarp2 encodes a negative regulator of the heat shock response and is required for maize embryogenesis
    Suneng Fu
    Department of Plant Biology, University of Georgia, Athens, Georgia 30602, USA
    Plant Cell 14:3119-32. 2002
    ..Recessive Mutator transposon mutations at the maize empty pericarp2 (emp2) locus led to dramatically increased expression of heat shock genes, retarded embryo development, and early-stage ..
  10. ncbi Asp338 controls hydride transfer in Escherichia coli IMP dehydrogenase
    K M Kerr
    Departments of Biochemistry and Biology, Brandeis University, Waltham, Massachusetts 02454, USA
    Biochemistry 39:9804-10. 2000
    IMP dehydrogenase (IMPDH) catalyzes the oxidation of IMP to XMP with the concomitant reduction of NAD(+). This reaction involves the formation of a covalent adduct with an active site Cys...
  11. pmc The tetraspan protein EMP2 modulates the surface expression of caveolins and glycosylphosphatidyl inositol-linked proteins
    Madhuri Wadehra
    Molecular Biology Institute, University of California at Los Angeles, Los Angeles, CA 90095, USA
    Mol Biol Cell 15:2073-83. 2004
    ..Epithelial membrane protein-2 (EMP2), a member of the four-transmembrane superfamily, facilitates plasma membrane delivery of certain integrins...
  12. ncbi The tetraspan protein epithelial membrane protein-2 interacts with beta1 integrins and regulates adhesion
    Madhuri Wadehra
    Molecular Biology Institute, The David Geffen School of Medicine at UCLA and Jonsson Comprehensive Cancer Center, 108ee Le Conte Avenue, Los Angeles, CA 90095, USA
    J Biol Chem 277:41094-100. 2002
    ..Epithelial membrane protein-2 (EMP2), a GAS3/PMP22 family member, was recently identified as a putative tumor suppressor gene...
  13. ncbi Crystal structure of Escherichia coli xanthine phosphoribosyltransferase
    S Vos
    Department of Biochemistry, The University of Queensland, Brisbane, Australia
    Biochemistry 36:4125-34. 1997
    ..1-pyrophosphate (PRib-PP) to the 6-oxopurine bases guanine, xanthine, and hypoxanthine to form GMP, XMP, and IMP, respectively...
  14. pmc De novo GMP synthesis is required for axon guidance in Drosophila
    Hong Long
    McGill Centre for Research in Neuroscience and Department of Neurology and Neurosurgery, McGill University Health Centre, Montreal, Quebec H3G 1A4, Canada
    Genetics 172:1633-42. 2006
    ..were observed when the raspberry (ras) gene, encoding for inosine monophosphate dehydrogenase catalyzing the IMP-to-XMP conversion in GMP de novo synthesis, was mutated...
  15. ncbi Isolation and sequence of a cDNA encoding mouse IMP dehydrogenase
    A A Tiedeman
    Seattle Biomedical Research Institute, WA 98109 1651
    Gene 97:289-93. 1991
    Inosinic acid (IMP) dehydrogenase (IMPD) catalyzes the conversion of IMP to XMP as the first committed step in GMP biosynthesis de novo...
  16. ncbi The first committed step reaction of caffeine biosynthesis: 7-methylxanthosine synthase is closely homologous to caffeine synthases in coffee (Coffea arabica L.)
    Kouichi Mizuno
    Faculty of Bioresource Sciences, Akita Prefectural University, Akita City, 010 0195, Akita, Japan
    FEBS Lett 547:56-60. 2003
    ..CmXRS1 was specific for xanthosine and xanthosine 5'-monophosphate (XMP) could not be used as a substrate. The K(m) value for xanthosine was 73.7 microM...
  17. ncbi Chromosomal mapping of Tmp (Emp1), Xmp (Emp2), and Ymp (Emp3), genes encoding membrane proteins related to Pmp22
    I Ben-Porath
    Department of Genetics, Hebrew University, Jerusalem, Israel
    Genomics 49:443-7. 1998
    ..in the Charcot-Marie-Tooth neuropathy, and three novel genes: TMP, XMP, and YMP (HGMW-approved symbols EMP1, EMP2 and EMP3, respectively)...
  18. ncbi The tetraspan protein EMP2 increases surface expression of class I major histocompatibility complex proteins and susceptibility to CTL-mediated cell death
    Madhuri Wadehra
    Department of Pathology and Laboratory Medicine, University of California at Los Angeles, Los Angeles, CA 90095 1722, USA
    Clin Immunol 107:129-36. 2003
    ..Epithelial membrane protein 2 (EMP2) is a tetraspan protein recently identified for its role in suppressing B lymphoma tumorigenicity...
  19. ncbi Metabolic fate of AMP, IMP, GMP and XMP in the cytosol of rat brain: an experimental and theoretical analysis
    A Torrecilla
    Departamento de Bioquimica, Instituto de Investigaciones Biomédicas Alberto Sols UAM CSIC, Facultad de Medicina, Madrid, Spain
    J Neurochem 76:1291-307. 2001
    A systematic study of the metabolic fate of AMP, IMP, GMP and XMP (NMP) in the presence of cytosol from rat brain is here presented; the kinetics of both disappearance of NMP, and appearance of their degradation products was followed by ..
  20. ncbi Crystallization and preliminary X-ray crystallographic studies of Escherichia coli xanthine phosphoribosyltransferase
    S Vos
    Centre for Protein Structure, Function and Engineering, University of Queensland, Australia
    J Struct Biol 116:330-4. 1996
    ..4.2.22) from Escherichia coli is a purine salvage enzyme which synthesizes the nucleotides GMP, XMP, and IMP...
  21. ncbi Crystal structures of Tritrichomonasfoetus inosine monophosphate dehydrogenase in complex with substrate, cofactor and analogs: a structural basis for the random-in ordered-out kinetic mechanism
    Glen L Prosise
    Department of Molecular Biology and Biochemistry University of California, Irvine, CA 92697 3900 USA
    J Mol Biol 326:517-27. 2003
    ..foetus have been solved: with its substrate IMP, IMP and the inhibitor mycophenolic acid (MPA), the product XMP with MPA, and XMP with the cofactor NAD(+). In addition, a potassium ion has been located at the dimer interface...
  22. ncbi Acid-base properties of xanthosine 5'-monophosphate (XMP) and of some related nucleobase derivatives in aqueous solution: micro acidity constant evaluations of the (N1)H versus the (N3)H deprotonation ambiguity
    Salah S Massoud
    Departement Chemie, Anorganische Chemie, Universitat Basel, Spitalstrasse 51, 4056 Basel, Switzerland
    Chemistry 10:5129-37. 2004
    The first acidity constant of fully protonated xanthosine 5'-monophosphate, that is, of H3(XMP)+, was estimated by means of a micro acidity constant scheme and the following three deprotonations of the H2(XMP)+/- (pKa=0.97), H(XMP)- (5...
  23. ncbi Substitution of the conserved Arg-Tyr dyad selectively disrupts the hydrolysis phase of the IMP dehydrogenase reaction
    Yollete V Guillen Schlippe
    Department of Biochemistry, Brandeis University, Waltham, Massachusetts 02454, USA
    Biochemistry 43:4511-21. 2004
    Inosine 5'-monophosphate dehydrogenase (IMPDH) catalyzes the oxidation of IMP to XMP via the covalent E-XMP* intermediate (E-XMP*), with the concomitant reduction of NAD(+)...
  24. pmc Functional consequences of interactions between FAK and epithelial membrane protein 2 (EMP2)
    Shawn A Morales
    Department of Pathology and Laboratory Medicine, University of California, Los Angeles, California, USA
    Invest Ophthalmol Vis Sci 50:4949-56. 2009
    Collagen gel contraction by ARPE-19 is controlled by epithelial membrane protein 2 (EMP2) through focal adhesion kinase (FAK) activation. The purpose of this study was to test the role of EMP2 in the cellular context of FAK activation.
  25. ncbi The immunosuppressive agent mizoribine monophosphate forms a transition state analogue complex with inosine monophosphate dehydrogenase
    Lu Gan
    Department of Biochemistry and Chemistry and the Rosenstiel Basic Medical Sciences Research Center, Brandeis University, Waltham, Massachusetts 02454, USA
    Biochemistry 42:857-63. 2003
    ..This enzyme catalyzes the oxidation of IMP to XMP with the concomitant reduction of NAD via a covalent intermediate at Cys319 (E-XMP)...
  26. ncbi Kinetic and biochemical characterization of Plasmodium falciparum GMP synthetase
    Javaid Yousuf Bhat
    Molecular Biology and Genetics Unit, Jawaharlal Nehru Centre for Advanced Scientific Research, Jakkur, Bangalore 560064, India
    Biochem J 409:263-73. 2008
    ..GMPS, a G-type amidotransferase, catalyses the amination of XMP to GMP with the reaction occurring in two domains, the GAT (glutamine amidotransferase) and ATPPase (ATP ..
  27. ncbi Validation and application of a high-performance liquid chromatographic-based assay for determination of the inosine 5'-monophosphate dehydrogenase activity in erythrocytes
    Philipe N Khalil
    Department of Surgery, Downtown Medical Centre, Ludwig Maximilians University, D 80336 Munich, Germany
    J Chromatogr B Analyt Technol Biomed Life Sci 842:1-7. 2006
    ..erythrocyte separation/haemolysis and assay conditions and a distinct segregation of xanthosine 5'-monophosphate (XMP) from the clear supernatant after precipitation...
  28. ncbi Structural properties of the neutral and monoanionic forms of xanthosine, highly relevant to their substrate properties with various enzyme systems
    Jarosław Poznański
    Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warszawa, Poland
    Nucleosides Nucleotides Nucleic Acids 22:249-63. 2003
    ..These findings are highly relevant to the modes of binding, and to the substrate properties, of Xan, Xao and its 5'-phosphate (XMP) in numerous enzyme systems, hitherto virtually ignored, and illustrated by several concrete examples.
  29. ncbi Crystal structure of a ternary complex of Tritrichomonas foetus inosine 5'-monophosphate dehydrogenase: NAD+ orients the active site loop for catalysis
    Lu Gan
    Department of Biochemistry, Brandeis University, Waltham, Massachusetts 02454, USA
    Biochemistry 41:13309-17. 2002
    Inosine 5'-monophosphate dehydrogenase (IMPDH) catalyzes the conversion of IMP to XMP with the reduction of NAD(+), which is the rate-limiting step in the biosynthesis of guanine nucleotides. IMPDH is a promising target for chemotherapy...
  30. ncbi The roles of conserved carboxylate residues in IMP dehydrogenase and identification of a transition state analog
    K M Kerr
    Department of Biochemistry, Brandeis University, Waltham, Massachusetts 02254, USA
    Biochemistry 36:13365-73. 1997
    IMP dehydrogenase (IMPDH) catalyzes the oxidation of IMP to XMP with the concomitant reduction of NAD+; the enzyme is activated by K+. This reaction is the rate-limiting step in de novo guanine nucleotide biosynthesis...
  31. pmc Identification of novel Mt-Guab2 inhibitor series active against M. tuberculosis
    Veeraraghavan Usha
    School of Biosciences, University of Birmingham, Edgbaston, Birmingham, United Kingdom
    PLoS ONE 7:e33886. 2012
    ..IMPDH exclusively catalyzes the conversion of inosine monophosphate (IMP) to xanthosine monophosphate (XMP) in the presence of the cofactor nicotinamide adenine dinucleotide (NAD(+))...
  32. pmc Epithelial membrane protein-2 promotes endometrial tumor formation through activation of FAK and Src
    Maoyong Fu
    Department of Pathology and Laboratory Medicine, University of California Los Angeles, Los Angeles, California, United States of America
    PLoS ONE 6:e19945. 2011
    ..One recent biomarker strongly associated with disease progression and survival is epithelial membrane protein-2 (EMP2), a tetraspan protein known to associate with and modify surface expression of certain integrin isoforms...
  33. doi EMP2 regulates angiogenesis in endometrial cancer cells through induction of VEGF
    L K Gordon
    Department of Ophthalmology, David Geffen School of Medicine at UCLA, Los Angeles, CA, USA
    Oncogene 32:5369-76. 2013
    ..In this study, we define a novel function for epithelial membrane protein-2 (EMP2) in the control of angiogenesis...
  34. doi IPH-926 lobular breast cancer cells are triple-negative but their microarray profile uncovers a luminal subtype
    Matthias Christgen
    Institute of Pathology, Hannover Medical School, Hannover, Germany
    Cancer Sci 104:1726-30. 2013
    ..Quantitative real-time RT-PCR confirmed enhanced expression of luminal marker genes (AGR2, CLU, CA12, EMP2, CLDN3) and low or absent expression of basal marker genes (KRT5, CD44, CAV1, VIM)...
  35. pmc Substrate activation and conformational dynamics of guanosine 5'-monophosphate synthetase
    Justin C Oliver
    Department of Medicinal Chemistry and Molecular Pharmacology, Purdue University, West Lafayette, Indiana 47907, United States
    Biochemistry 52:5225-35. 2013
    ..responsible for the last step in the guanosine branch of the pathway, the amination of xanthosine 5'-monophosphate (XMP)...
  36. doi Substrate-induced conformational changes in Plasmodium falciparum guanosine monophosphate synthetase
    Javaid Y Bhat
    Molecular Biology and Genetics Unit, Jawaharlal Nehru Centre for Advanced Scientific Research, Bangalore, India
    FEBS J 278:3756-68. 2011
    ..amidotransferase that incorporates ammonia derived from glutamine into the nucleotide xanthosine 5'-monophosphate (XMP) to form guanosine 5'-monophosphate (GMP)...
  37. ncbi Human GMP synthetase
    T Page
    Int J Biochem 16:117-20. 1984
    ..The Km values were determined to be 4.9 microM for XMP, 270 microM for ATP, and 340 microM for glutamine...
  38. pmc Defects in purine nucleotide metabolism lead to substantial incorporation of xanthine and hypoxanthine into DNA and RNA
    Bo Pang
    Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Proc Natl Acad Sci U S A 109:2319-24. 2012
    ..we observed large increases (up to 600-fold) in hypoxanthine in both DNA and RNA in cells unable to convert IMP to XMP or AMP (IMP dehydrogenase, guaB; adenylosuccinate synthetase, purA, and ADE12), and unable to remove dITP/ITP and ..
  39. ncbi Differential effects of bactericidal/permeability-increasing protein (BPI) analogues on retinal neovascularization and retinal pericyte growth
    Ravi K Rauniyar
    Research Division, Joslin Diabetes Center, Boston, Massachusetts 02215, USA
    Invest Ophthalmol Vis Sci 43:503-9. 2002
    ..retinopathies, the antiangiogenic activity of a human recombinant 21-kDa modified N-terminal fragment of BPI (rBPI(21)), which has the biological properties of the holoprotein, and a peptidomimetic (XMP.Z) derived from BPI were examined.
  40. ncbi Guanidine derivatives rescue the Arg418Ala mutation of Tritrichomonas foetus IMP dehydrogenase
    Yollete V Guillen Schlippe
    Department of Biochemistry, Brandeis University, Waltham, Massachusetts 02454, USA
    Biochemistry 44:16695-700. 2005
    IMP dehydrogenase (IMPDH) catalyzes the oxidation of inosine 5'-monophosphate (IMP) to xanthosine 5'-monophosphate (XMP) and the reduction of NAD(+)...
  41. ncbi Positional isotope exchange and kinetic experiments with Escherichia coli guanosine-5'-monophosphate synthetase
    W von der Saal
    Biochemistry 24:5343-50. 1985
    ..The initial velocity patterns of MgATP, XMP, and either NH3 or glutamine (as nitrogen source) were consistent with the ordered addition of MgATP followed by ..
  42. pmc Loss of epithelial membrane protein-2 expression confers an independent prognosticator in nasopharyngeal carcinoma: a cohort study
    Yi Hsien Chen
    Department of Emergency Medicine, Chi Mei Medical Center, Tainan, Taiwan
    BMJ Open 2:e000900. 2012
    To evaluate the expression of epithelial membrane protein-2 (EMP2) protein and its clinicopathological associations in patients with nasopharyngeal carcinoma.
  43. ncbi Effect of diabetes mellitus on mycophenolate sodium pharmacokinetics and inosine monophosphate dehydrogenase activity in stable kidney transplant recipients
    Chirag G Patel
    Department of Biomedical and Pharmaceutical Sciences, University of Rhode Island, Kingston, Rhode Island 02881, USA
    Ther Drug Monit 29:735-42. 2007
    ..In contrast, IMPDH activity was 17.5+/-2.8 versus 46.6+/-2.5 nmol XMP/h/microg protein in diabetics and nondiabetics, respectively (P<0...
  44. ncbi The tetraspan protein EMP2 regulates expression of caveolin-1
    Ashley Forbes
    Molecular Biology Institute, David Geffen School of Medicine, UCLA, Los Angeles, California 90095, USA
    J Biol Chem 282:26542-51. 2007
    ..b>EMP2 (epithelial membrane protein 2) is a tetraspan protein recently identified as a novel regulator of caveolin-1 ..
  45. doi IMP dehydrogenase is recruited to the transcription complex through serine 2 phosphorylation of RNA polymerase II
    Jae Hyun Park
    Department of Biochemistry and Molecular Biology, College of Pharmacy, Sungkyunkwan University, 300 Cheoncheon dong, Jangan gu, Suwon, Gyeonggi do 440 746, Republic of Korea
    Biochem Biophys Res Commun 392:588-92. 2010
    ..IMPDH) catalyzes the rate-limiting step in the de novo synthesis of guanine, namely the oxidation of IMP to XMP with a concomitant reduction of NAD+...
  46. pmc Rewiring integrin-mediated signaling and cellular response with the peripheral myelin protein 22 and epithelial membrane protein 2 components of the tetraspan web
    Shawn A Morales
    Department of Ophthalmology, University of California at Los Angeles, Los Angeles, California, USA
    Invest Ophthalmol Vis Sci 52:5465-72. 2011
    ..Expression of the tetraspan protein epithelial membrane protein 2 (EMP2) controls gel contraction through FAK activation...
  47. ncbi Kinetic characterization of inosine monophosphate dehydrogenase of Leishmania donovani
    Fredrick Dobie
    Institute of Parasitology, Macdonald Campus of McGill University, 21, 111 Lakeshore Road, Ste Anne de Bellevue, Quebec, Canada H9X 3V9
    Mol Biochem Parasitol 152:11-21. 2007
    ..Kinetic studies revealed that XMP and GMP were inhibitors with K(i) values of approximately 26 and 210 microM, respectively, suggesting that these ..
  48. ncbi [Cloning and characterization of the gene encoding IMPDH of Streptococcus suis serotype 2]
    Zhi Tao Duan
    Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China
    Wei Sheng Wu Xue Bao 46:730-3. 2006
    ..that the protein has IMPDH function and can catalyze the rate-limiting reaction of GTP biosynthesis, the NAD-dependent reduction of IMP into XMP. Flow cytometry (FCM) revealed that the protein had apparent effect on HEp-2 cell cycle.
  49. ncbi The effects of removing the GAT domain from E. coli GMP synthetase
    Jessica L Abbott
    Department of Chemistry, Lebanon Valley College, 101 N College Ave, Annville, PA 17003 1400, USA
    Protein J 25:483-91. 2006
    E. coli GMP synthetase (GMPS) catalyzes the conversion of XMP to GMP...
  50. doi Cofactor mobility determines reaction outcome in the IMPDH and GMPR (β-α)8 barrel enzymes
    Gregory C Patton
    Department of Biology, Brandeis University, Waltham, Massachusetts, USA
    Nat Chem Biol 7:950-8. 2011
    ..Here we show that the GMPR reaction uses the same intermediate E-XMP* as IMPDH, but in this reaction the intermediate reacts with ammonia instead of water...
  51. pmc Kinetically controlled drug resistance: how Penicillium brevicompactum survives mycophenolic acid
    Xin E Sun
    Graduate Program in Biochemistry, Brandeis University, Waltham, Massachusetts 02453, USA
    J Biol Chem 286:40595-600. 2011
    ..IMPDH catalyzes the conversion of IMP to XMP via a covalent enzyme intermediate, E-XMP*; MPA inhibits by trapping E-XMP*. P...
  52. doi Structure and inhibition of orotidine 5'-monophosphate decarboxylase from Plasmodium falciparum
    David B Langley
    School of Molecular and Microbial Biosciences, University of Sydney, Sydney, New South Wales 2006, Australia
    Biochemistry 47:3842-54. 2008
    ..6 +/- 0.7 nM) > xanthosine 5'-monophosphate (XMP, K i = 4.4 +/- 0...
  53. doi Crystal structure of the ATPPase subunit and its substrate-dependent association with the GATase subunit: a novel regulatory mechanism for a two-subunit-type GMP synthetase from Pyrococcus horikoshii OT3
    Shintaro Maruoka
    Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, University of Tokyo, 1 1 1 Yayoi, Bunkyo ku, Tokyo 113 8657, Japan
    J Mol Biol 395:417-29. 2010
    ..yield glutamate and ammonia, while ATPPase utilizes ammonia to convert adenyl xanthosine 5'-monophosphate (adenyl-XMP) into guanosine 5'-monophosphate...
  54. doi Xanthosine 5'-monophosphate (XMP). Acid-base and metal ion-binding properties of a chameleon-like nucleotide
    Helmut Sigel
    Department of Chemistry, Inorganic Chemistry, University of Basel, Spitalstrasse 51, CH 4056 Basel, Switzerland
    Chem Soc Rev 38:2465-94. 2009
    The four acidity constants of threefold protonated xanthosine 5'-monophosphate, H(3)(XMP)(+), reveal that in the physiological pH range around 7...
  55. pmc Epithelial membrane protein-2 expression is an early predictor of endometrial cancer development
    Omar Habeeb
    Department of Pathology and Laboratory Medicine, David Geffen School of Medicine, University of California at Los Angeles, Los Angeles, California, USA
    Cancer 116:4718-26. 2010
    ..Previously, the authors have shown that the tetraspan protein epithelial membrane protein-2 (EMP2) is a prognostic indicator for EC aggressiveness and survival...
  56. pmc Epithelial membrane protein-2 is a novel therapeutic target in ovarian cancer
    Maoyong Fu
    Department of Pathology and Laboratory Medicine, David Geffen School of Medicine at UCLA, Los Angeles, CA 90095, USA
    Clin Cancer Res 16:3954-63. 2010
    The tetraspan protein epithelial membrane protein-2 (EMP2) has been shown to regulate the surface display and signaling from select integrin pairs, and it was recently identified as a prognostic biomarker in human endometrial cancer...
  57. doi Identification of the myostatin locus (MSTN) as having a major effect on optimum racing distance in the Thoroughbred horse in the USA
    M M Binns
    Equine Analysis, PO Box 4570, Midway, KY 40347, USA
    Anim Genet 41:154-8. 2010
    ..and under compared to 8-10 furlongs) identified a locus on ECA18 that was statistically significant (-log 10 EMP2=1.63) at the genome-wide level following permutation analysis (10,000 permutations)...
  58. pmc Blockade of epithelial membrane protein 2 (EMP2) abrogates infection of Chlamydia muridarum murine genital infection model
    Kaori Shimazaki
    Molecular Biology Institute, David Geffen School of Medicine at UCLA, Los Angeles, CA, USA
    FEMS Immunol Med Microbiol 55:240-9. 2009
    ..Blockade of epithelial membrane protein 2 (EMP2) by genetic silencing or neutralizing polyclonal antibody reduced chlamydial infectivity in vitro...
  59. pmc Diabodies targeting epithelial membrane protein 2 reduce tumorigenicity of human endometrial cancer cell lines
    Kaori Shimazaki
    Molecular Biology Institute, University of California, Los Angeles, CA 90095, USA
    Clin Cancer Res 14:7367-77. 2008
    ..One promising biomarker is epithelial membrane protein 2 (EMP2), and its expression is an independent prognostic indicator for tumors with poor clinical outcome expression...
  60. pmc FAK activation and the role of epithelial membrane protein 2 (EMP2) in collagen gel contraction
    Shawn A Morales
    Department of Pathology and Laboratory Medicine, University of California, Los Angeles, California, USA
    Invest Ophthalmol Vis Sci 50:462-9. 2009
    ..Since EMP2 is highly expressed in retinal pigment epithelium, this study investigates the role of EMP2 in collagen gel ..
  61. doi Cellular IMPDH enzyme activity is a potential target for the inhibition of Chikungunya virus replication and virus induced apoptosis in cultured mammalian cells
    M Khan
    Division of Virology, Defence Research and Development Establishment, Jhansi Road, Gwalior 474002, MP, India
    Antiviral Res 89:1-8. 2011
    ..catalyzes an essential step in the de novo biosynthesis of guanine nucleotide, namely, the conversion of IMP to XMP. The depletion of the intracellular GTP and dGTP pools is the major event occurring in the cells exposed to the ..
  62. pmc Family-based genome-wide association scan of attention-deficit/hyperactivity disorder
    Eric Mick
    Massachusetts General Hospital, 55 Fruit Street Warren 705, Boston, MA 02114, USA
    J Am Acad Child Adolesc Psychiatry 49:898-905.e3. 2010
    ..We have conducted a third, independent, multisite GWAS of DSM-IV-TR ADHD...
  63. ncbi Inhibition of IMPDH by mycophenolic acid: dissection of forward and reverse pathways using capillary electrophoresis
    M A Fleming
    Vertex Pharmaceuticals Incorporated, Cambridge, Massachusetts 02139 4211, USA
    Biochemistry 35:6990-7. 1996
    ..Slow inhibition, without substrate turnover, was achieved by incubating free enzyme with excess XMP and MPA...
  64. doi Hypoxanthine guanine phosphoribosyltransferase distorts the purine ring of nucleotide substrates and perturbs the pKa of bound xanthosine monophosphate
    Spriha Gogia
    National Centre for Biological Sciences, TIFR, GKVK Campus, Bellary Road, Bangalore 560065, India
    Biochemistry 50:4184-93. 2011
    ..We used ultraviolet resonance Raman spectroscopy to study the complexes of HGPRT with products (IMP, GMP, and XMP), in both organisms, in resonance with the purine nucleobase electronic absorption...
  65. doi Epithelial membrane protein-2 (EMP2) and experimental proliferative vitreoretinopathy (PVR)
    David G Telander
    Department of Ophthalmology, University of California Davis, Sacramento, California, USA
    Curr Eye Res 36:546-52. 2011
    ..In an in vitro collagen-gel contraction assay, epithelial membrane protein 2 (EMP2) controls contraction through activation of focal adhesion kinase (FAK) in a RPE cell line (ARPE-19)...
  66. pmc IMP dehydrogenase deficiency in Leishmania donovani causes a restrictive growth phenotype in promastigotes but is not essential for infection in mice
    Audrey L Fulwiler
    Department of Biochemistry and Molecular Biology, Oregon Health and Science University, 3181 SW Sam Jackson Park Road, Portland, OR 97239 3098, USA
    Mol Biochem Parasitol 180:123-6. 2011
    ..a three-step pathway that starts with the deamination of guanine to xanthine, followed by phosphoribosylation to XMP and then conversion to GMP; or (3) direct guanine phosphoribosylation by HGPRT...
  67. doi Structural diversity and plasticity associated with nucleotides targeting orotidine monophosphate decarboxylase
    Ewa Poduch
    Center for Molecular Design and Preformulations, Toronto General Research Institute, Toronto General Hospital, Toronto, ON, Canada
    J Med Chem 51:432-8. 2008
    ..UMP and XMP exhibited higher affinities as compared to the other nucleotides tested...
  68. ncbi Acid-base and metal-ion-binding properties of xanthosine 5'-monophosphate (XMP) in aqueous solution: complex stabilities, isomeric equilibria, and extent of macrochelation
    Helmut Sigel
    Department of Chemistry, Inorganic Chemistry, University of Basel, Spitalstrasse 51, 4056 Basel, Switzerland
    Chemistry 12:8106-22. 2006
    The four acidity constants of threefold protonated xanthosine 5'-monophosphate, H3(XMP)+, reveal that at the physiological pH of 7...
  69. ncbi Knockdown of the tetraspan protein epithelial membrane protein-2 inhibits implantation in the mouse
    Madhuri Wadehra
    Molecular Biology Institute, Department of Pathology and Laboratory Medicine, University of California at Los Angeles, Los Angeles, CA 90095, USA
    Dev Biol 292:430-41. 2006
    ..In this study, we demonstrate that the tetraspan protein epithelial membrane protein-2 (EMP2) is involved in these molecular interactions during implantation...
  70. ncbi Acid-base catalysis in the chemical mechanism of inosine monophosphate dehydrogenase
    G D Markham
    Institute for Cancer Research, Fox Chase Cancer Center, Philadelphia, Pennsylvania 19111, USA
    Biochemistry 38:4433-40. 1999
    Inosine-5'-monophosphate dehydrogenase (IMPDH) catalyzes the K+-dependent reaction IMP + NAD + H2O --> XMP + NADH + H+ which is the rate-limiting step in guanine nucleotide biosynthesis...
  71. ncbi N2-hydroxyguanosine 5'-monophosphate is a time-dependent inhibitor of Escherichia coli guanosine monophosphate synthetase
    M L Deras
    Department of Medicinal Chemistry and Molecular Pharmacology, Purdue University, West Lafayette, Indiana 47907 1333, USA
    Biochemistry 38:303-10. 1999
    ..One untested hypothesis was that hydroxylamine serves as a substrate and intercepts a xanthosine 5'-monophosphate- (XMP-) adenylate intermediate in the enzyme active site...
  72. ncbi Metabolic effects of thiopurine derivatives against human CCRF-CEM leukaemia cells
    R Z Shi
    Department of Biochemistry, University of Sydney, NSW, Australia
    Int J Biochem Cell Biol 30:885-95. 1998
    ....
  73. ncbi Crystal structure of Tritrichomonas foetus inosine-5'-monophosphate dehydrogenase and the enzyme-product complex
    F G Whitby
    Department of Pharmaceutical Chemistry, School of Pharmacy, University of California, San Francisco, 94143 0446, USA
    Biochemistry 36:10666-74. 1997
    ..The enzyme catalyzes the oxidation of inosine monophosphate (IMP) to xanthosine monophosphate (XMP), the committed step in de novo guanosine monophosphate (GMP) biosynthesis...
  74. ncbi High level expression of XMP aminase in Escherichia coli and its application for the industrial production of 5'-guanylic acid
    T Fujio
    Planning and Development Department Food, Liquor and Food Division, Kyowa Hakko Kogya Co, Ltd, Tokyo, Japan
    Biosci Biotechnol Biochem 61:840-5. 1997
    To improve the efficiency of the enzymatic conversion of 5'-xanthylic acid (XMP) to 5'-guanylic acid (GMP), we attempted to increase the activity of the conversion enzyme, XMP aminase (GMP synthetase) encoded by the guaA gene in ..
  75. ncbi Characterization of a tumor-associated gene, a member of a novel family of genes encoding membrane glycoproteins
    I Ben-Porath
    Department of Genetics, Hebrew University of Jerusalem, Givat Ram, Israel
    Gene 183:69-75. 1996
    ..In our search for human sequences homologous to TMP and PMP22, we identified two new genes which we have named XMP and YMP...
  76. ncbi Inactivation of inosine 5'-monophosphate dehydrogenase by the antiviral agent 5-ethynyl-1-beta-D-ribofuranosylimidazole-4-carboxamide 5'-monophosphate
    W Wang
    Graduate Department of Biochemistry, Brandeis University, Waltham, Massachusetts 02254, USA
    Biochemistry 35:95-101. 1996
    ..IMPDH converts inosine 5'-monophosphate (IMP) to xanthosine 5'-monophosphate (XMP) with concomitant conversion of NAD+ to NADH...
  77. ncbi Preliminary X-ray analysis of Escherichia coli GMP synthetase: determination of anomalous scattering factors for a cysteinyl mercury derivative
    J J Tesmer
    Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907
    Proteins 18:394-403. 1994
    ..GMPS catalyzes the conversion of XMP to GMP in the final step of de novo guanine nucleotide biosynthesis, and is a member of the glutamine ..
  78. ncbi Biochemical characterization of human GMP synthetase
    J Nakamura
    Institute of Biochemistry and Cell Biology, Syntex Discovery Research, Palo Alto, California 94304, USA
    J Biol Chem 270:7347-53. 1995
    ..Interaction of GMP synthetase with xanthosine 5'-monophosphate (XMP), a substrate, exhibits sigmoidal kinetics with a Hill coefficient of 1.48 +/- 0.07...
  79. ncbi Allelic differences in the VHOx-1 gene explain the absence of a B cell clonal dominance in the primary response of C57BL/6 mice to phthalate
    D B Winter
    Department of Molecular Immunology, Rosewell Park Cancer Institute, Unit of the New York State Department of Health, Buffalo 14263, USA
    J Immunol 155:2445-52. 1995
    ..A highly conserved Id (CRIXmp-1) associated with the murine (BALB/c) humoral immune response to the hapten phthalate (Xmp) is conspicuously absent in C57BL/6 mice...
  80. ncbi Purification and characterization of GMP synthetase from Yoshida sarcoma ascites cells
    K Hirai
    Division of Protein Metabolism, Osaka University
    J Biochem 102:893-902. 1987
    ..Its isoelectric point was estimated to be 5.6. The Km values of this enzyme for XMP, ATP, and glutamine were calculated to be 4.6, 120, and 300 microM, respectively...
  81. ncbi Dual effects of pyrazofurin and 3-deazauridine upon pyrimidine and purine biosynthesis in mouse L1210 leukemia
    M E Sant
    Department of Biochemistry, University of Sydney, Australia
    Cancer Res 49:2645-50. 1989
    ..concentrations of N-succino-AICAR (SAICAR), AICAR, 5-formamidoimidazole-4-carboxamide ribotide (FAICAR), IMP, XMP, and GMP at later times indicate that AICAR transformylase is not significantly inhibited in cultured cells; rather ..
  82. ncbi Increased activity, amount, and altered kinetic properties of IMP dehydrogenase from mycophenolic acid-resistant neuroblastoma cells
    S D Hodges
    Department of Medical Biochemistry, Faculty of Medicine, University of Calgary, Alberta, Canada
    J Biol Chem 264:18137-41. 1989
    ..4 nM to 3.4 microM for the enzyme from NB versus NB-Myco cells, and the Ki for XMP was increased 4-fold from 78 to 336 microM...
  83. ncbi Mycophenolic acid and thiazole adenine dinucleotide inhibition of Tritrichomonas foetus inosine 5'-monophosphate dehydrogenase: implications on enzyme mechanism
    L Hedstrom
    Department of Pharmaceutical Chemistry, School of Pharmacy, University of California, San Francisco 94143
    Biochemistry 29:849-54. 1990
    ..dehydrogenase (IMPDH) catalyzes the oxidation of inosine 5'-monophosphate (IMP) to xanthosine 5'-monophosphate (XMP) with the conversion of NAD to NADH...
  84. ncbi A novel mechanism of mycophenolic acid resistance in the protozoan parasite Tritrichomonas foetus
    L Hedstrom
    Department of Pharmaceutical Chemistry, School of Pharmacy, University of California, San Francisco 94143
    Biochem Pharmacol 39:151-60. 1990
    ..Xanthine incorporation via XMP provided an IMP dehydrogenase independent route to guanine nucleotides that would enable the parasite to become ..
  85. ncbi Regional localization of the human epithelial membrane protein genes 1, 2, and 3 (EMP1, EMP2, EMP3) to 12p12.3, 16p13.2, and 19q13.3
    T Liehr
    Institute of Human Genetics, Schwabachanlage 10, Erlangen, D 91054, Germany
    Genomics 58:106-8. 1999
  86. ncbi Structure-based identification of a novel NTPase from Methanococcus jannaschii
    K Y Hwang
    Structural Biology Center, Korea Institute of Science and Technology, Seoul, South Korea
    Nat Struct Biol 6:691-6. 1999
    ..analysis shows that Mj0226 protein is a novel nucleotide triphosphatase that can efficiently hydrolyze nonstandard nucleotides such as XTP to XMP or ITP to IMP, but not the standard nucleotides, in the presence of Mg2+ or Mn2+ ions.
  87. ncbi Spatial contaminant heterogeneity: quantification with scale of measurement at contrasting sites
    Paul D Taylor
    Centre for Environmental Research, School of Life Sciences, University of Sussex, Falmer, Brighton, UKBN1 9QJ
    J Environ Monit 7:1364-70. 2005
    ..In situ portable X-ray fluorescence (PXRF) and X-ray microprobe (XMP) techniques were employed to test the feasibility of this approach...
  88. pmc Candidate-gene screening and association analysis at the autism-susceptibility locus on chromosome 16p: evidence of association at GRIN2A and ABAT
    Gabrielle Barnby
    Wellcome Trust Centre for Human Genetics, University of Oxford, Oxford, United Kingdom
    Am J Hum Genet 76:950-66. 2005
    ..10 positional candidate genes that map to 16p11-13 were examined for coding variants: A2BP1, ABAT, BFAR, CREBBP, EMP2, GRIN2A, MRTF-B, SSTR5, TBX6, and UBN1...
  89. ncbi [Activation of the alternative oxidase of Yarrowia lipolytica by adenosine 5'-monophosphate]
    A G Medentsev
    Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, pr Nauki 5, Pushchino, Moscow Oblast, 142290 Russia
    Mikrobiologiia 73:149-56. 2004
    ..amp = gmp > gdp > gtp > xmp > imp...
  90. ncbi Transcription status of vaccine candidate genes of Plasmodium falciparum during the hepatic phase of its life cycle
    Myriam Bodescot
    INSERM U511, Immunobiologie Cellulaire et Moleculaire des Infections Parasitaires, Centre Hospitalier Universitaire Pitie Salpetriere, Universite Pierre et Marie Curie, 91 Boulevard de l Hopital, 75643 Paris Cedex 13, France
    Parasitol Res 92:449-52. 2004
    The CSP, EMP2/MESA, MSP2, MSP3, MSP5, RAP1, RAP2, RESA1, SERA1 and SSP2/TRAP genes of Plasmodium falciparum are vaccine candidates...
  91. ncbi Structural insights into the human and avian IMP cyclohydrolase mechanism via crystal structures with the bound XMP inhibitor
    Dennis W Wolan
    Department of Molecular Biology and The Skaggs Institute for Chemical Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA
    Biochemistry 43:1171-83. 2004
    ..we focus on the IMPCH active site and the cyclohydrolase mechanism through comparison of crystal structures of XMP inhibitor complexes of human ATIC at 1.9 A resolution with the previously determined avian enzyme...
  92. ncbi Crystal structures of inhibitor complexes reveal an alternate binding mode in orotidine-5'-monophosphate decarboxylase
    Ning Wu
    Department of Biochemistry, University of Toronto, Ontario M5S 1A8, Canada
    J Biol Chem 277:28080-7. 2002
    ..thermoautotrophicum complexed with its product UMP and the inhibitors 6-hydroxyuridine 5'-phosphate (BMP), XMP, and CMP are reported...
  93. ncbi 31P NMR studies of energy metabolism in xanthosine-5'-monophosphate overproducing Corynebacterium ammoniagenes
    Yasushi Noguchi
    Fermentation and Biotechnology Laboratories and Central Research Laboratories, Ajinomoto Co, Inc, Kawasaki ku, Kawasaki, Kanagawa, Japan
    Eur J Biochem 270:2622-6. 2003
    Corynebacterium ammoniagenes is an overproducer of xanthosine-5'-monophosphate (XMP) by consuming either glcose (glc) or glutamic acid (glu)...
  94. pmc Escherichia coli phnN, encoding ribose 1,5-bisphosphokinase activity (phosphoribosyl diphosphate forming): dual role in phosphonate degradation and NAD biosynthesis pathways
    Bjarne Hove-Jensen
    Department of Biological Chemistry, Institute of Molecular Biology, University of Copenhagen, Copenhagen, Denmark
    J Bacteriol 185:2793-801. 2003
    ..the ribose 1,5-bisphosphokinase activity to the activity of xanthine phosphoribosyltransferase in the presence of xanthine, which resulted in the formation of 5'-XMP, and by cochromatography of the reaction product with authentic PRPP.
  95. ncbi Analysis of purine nucleotides in lymphocytes from healthy subjects and AIDS patients
    A Tabucchi
    Istituto di Biochimica e di Enzimologia, , Italy
    Biomed Pharmacother 46:25-9. 1992
    The most important purine nucleotides (NAD, AMP, IMP, GMP, XMP, ADP, ATP, GDP, GTP) were analyzed by HPLC in the lymphocytes of healthy subjects and HIV-1 seropositive patients at different stages of the disease (ARC-AIDS)...
  96. pmc Broad-spectrum antiviral activity of the IMP dehydrogenase inhibitor VX-497: a comparison with ribavirin and demonstration of antiviral additivity with alpha interferon
    W Markland
    Vertex Pharmaceuticals Inc, Cambridge, Massachusetts 02139 4242, USA
    Antimicrob Agents Chemother 44:859-66. 2000
    ..catalyzes an essential step in the de novo biosynthesis of guanine nucleotides, namely, the conversion of IMP to XMP. The major event occurring in cells exposed to competitive IMPDH inhibitors such as ribavirin or uncompetitive ..
  97. ncbi Crystal structure at 2.4 A resolution of Borrelia burgdorferi inosine 5'-monophosphate dehydrogenase: evidence of a substrate-induced hinged-lid motion by loop 6
    F M McMillan
    The Rosenstiel Basic Medical Sciences Research Center and Department of Biochemistry, Brandeis University, Waltham, Massachussetts 02454, USA
    Biochemistry 39:4533-42. 2000
    The conversion of inosine 5'-monophosphate (IMP) to xanthosine 5'-monophosphate (XMP) is the committed and rate-limiting reaction in de novo guanine nucleotide biosynthesis...
  98. ncbi Influence of mycophenolic acid on inosine 5'-monophosphate dehydrogenase activity in human peripheral blood mononuclear cells
    Heide Daxecker
    Institute of Laboratory Diagnostics and Ludwig Boltzmann Institute for Cardiothoracic Research, Kaiser Franz Josef Hospital, Kundratstrasse 3, A 1100 Vienna, Austria
    Clin Chim Acta 318:71-7. 2002
    ..dehydrogenase (IMPDH) catalyses the oxidation of inosine 5'-monophosphate (IMP) to xanthosine 5'-monophosphate (XMP)...
  99. ncbi Non-radioactive determination of inosine 5'-monophosphate dehydro-genase (IMPDH) in peripheral mononuclear cells
    P Glander
    Department of Internal Medicine, Nephrology, University Medical Center Charite, Campus Mitte, Humboldt University, Schumannstr 20 21, 10098 Berlin, Germany
    Clin Biochem 34:543-9. 2001
    ..It was the aim of this study to develop a nonradioactive method for specific measurement of IMPDH activity in isolated peripheral mononuclear cells (MNC)...
  100. ncbi Use of cDNA microarrays to analyze dioxin-induced changes in human liver gene expression
    F W Frueh
    Department of Molecular Pharmacology, Stanford University School of Medicine, Stanford, CA 95305 5332, USA
    Toxicol Lett 122:189-203. 2001
    ..Most changes are relatively subtle (two- to four-fold). We verified the regulation of protooncogene cot, XMP, and human enhancer of filamentation-1 (HEF1), genes involved in cellular proliferation, as well as metallothionein,..
  101. pmc Mapping of Charcot-Marie-Tooth disease type 1C to chromosome 16p identifies a novel locus for demyelinating neuropathies
    Valerie A Street
    V M Bloedel Hearing Research Center, Department of Otolaryngology Head and Neck Surgery, University of Washington, Seattle, WA 98195, USA
    Am J Hum Genet 70:244-50. 2002
    ..The epithelial membrane protein 2 gene (EMP2), which maps to chromosome 16p13.2, was evaluated as a candidate gene for CMT1C.

Research Grants62

  1. INOSINE MONOPHOSPHATE DEHYDROGENASE
    Lizbeth Hedstrom; Fiscal Year: 2001
    ..IMPDH catalyzes the conversion of IMP to XMP with the concomitant reduction of NAD...
  2. IMP Dehydrogenase
    LIZBETH K HEDSTROM; Fiscal Year: 2010
    ..dehydrogenase (IMPDH) catalyzes the oxidation of inosine monophosphate (IMP) to xanthosine monophosphate (XMP) with the concomitant reduction of NAD+...
  3. IMP Dehydrogenase
    Lizbeth Hedstrom; Fiscal Year: 2007
    ..dehydrogenase (IMPDH) catalyzes the oxidation of inosine monophosphate (IMP) to xanthosine monophosphate (XMP) with the concomitant reduction of NAD+...
  4. IMP Dehydrogenase
    Lizbeth Hedstrom; Fiscal Year: 2006
    ..step in guanine nucleotide biosynthesis: the conversion of inosine monophosphate (IMP) to xanthosine monophosphate (XMP) with concomitant reduction of NAD...
  5. Novel Therapies to Prevent Blindness Caused by Proliferative Vitreoretinopathy
    David G Telander; Fiscal Year: 2010
    ..Recent studies support the hypothesis that control EMP2 (epithelial membrane protein 2), or its downstream signaling pathway, may change the biologic response of RPE cells ..
  6. Abnormal EMPS Expression Affects Pregnancy Outcome
    Jonathan Braun; Fiscal Year: 2006
    ..identified, on the surface of uterine epithelium, a four transmembrane protein, epithelial membrane protein 2 (EMP2) that is required for successful implantation...
  7. ENZYMATIC MECHANISMS OF SULFUR NUCLEOSIDE METABOLISM
    GEORGE MARKHAM; Fiscal Year: 2007
    ..These studies will unmask why nature conserves use of a pyruvoyl cofactor for this metabolic function instead of adopting the common pyridoxal cofactor. ..
  8. IMP Dehydrogenase and the Hydra of Cancer Chemotherapy
    GEORGE MARKHAM; Fiscal Year: 2007
    ..the rate-limiting step in guanine nucleotide biosynthesis, the oxidation of IMP to xanthosine monophosphate (XMP)...
  9. Structure-Function Studies of the Proton-Gated Urea Channel from H. Pylori
    Hartmut Luecke; Fiscal Year: 2009
    ....
  10. Structure-Function Studies of the Proton-Gated Urea Channel from H. Pylori
    Hartmut Luecke; Fiscal Year: 2010
    ....
  11. ENZYMATIC MECHANISMS OF SULFUR NUCLEOSIDE METABOLISM
    GEORGE MARKHAM; Fiscal Year: 2003
    ....
  12. ENZYMATIC MECHANISMS OF SULFUR-NUCLEOSIDE METABOLISM
    GEORGE MARKHAM; Fiscal Year: 1993
    ..The roles of the divalent metal ions which are required for activity will be determined from paramagnetic resonance experiments which will reveal the ligands to the metal ions and the distance between them...