ELANE

Summary

Gene Symbol: ELANE
Description: elastase, neutrophil expressed
Alias: ELA2, HLE, HNE, PMN-E, SCN1, neutrophil elastase, PMN elastase, bone marrow serine protease, elastase 2, neutrophil, elastase-2, granulocyte-derived elastase, leukocyte elastase, medullasin, polymorphonuclear elastase
Species: human

Top Publications

  1. ncbi HIV-1 preferentially binds receptors copatched with cell-surface elastase
    Cynthia L Bristow
    Laboratory of Cellular Physiology and Immunology, The Rockefeller University and Population Council, New York, NY 10021, USA
    Blood 102:4479-86. 2003
  2. ncbi G-CSF induces stem cell mobilization by decreasing bone marrow SDF-1 and up-regulating CXCR4
    Isabelle Petit
    Department of Immunology, The Weizmann Institute of Science, Rehovot, Israel
    Nat Immunol 3:687-94. 2002
  3. ncbi Modulation of tissue factor and tissue factor pathway inhibitor-1 by neutrophil proteases
    Birgit A Steppich
    Deutsches Herzzentrum der Technischen Universität München, Lazarettstr 36, 80636 München, Germany
    Thromb Haemost 100:1068-75. 2008
  4. pmc Is lysyl oxidase-like protein-1, alpha-1 antitrypsin, and neutrophil elastase site specific in pelvic organ prolapse?
    Weng Chi Man
    Department of Obstetrics and Gynecology, Stanford University School of Medicine, 300 Pasteur Drive, HH 333, Stanford, CA 94305 5317, USA
    Int Urogynecol J Pelvic Floor Dysfunct 20:1423-9. 2009
  5. pmc Mechanism of heparin acceleration of tissue inhibitor of metalloproteases-1 (TIMP-1) degradation by the human neutrophil elastase
    Gabriel L C Nunes
    Centro Interdisciplinar de Investigação Bioquímica, Universidade de Mogi das Cruzes, Mogi das Cruzes, Brazil
    PLoS ONE 6:e21525. 2011
  6. doi Exploring Tagetes erecta Linn flower for the elastase, hyaluronidase and MMP-1 inhibitory activity
    Niladri Maity
    School of Natural Product Studies, Department of Pharmaceutical Technology, Jadavpur University, Kolkata 700032, India
    J Ethnopharmacol 137:1300-5. 2011
  7. ncbi Mutants of plasminogen activator inhibitor-1 designed to inhibit neutrophil elastase and cathepsin G are more effective in vivo than their endogenous inhibitors
    Steingrimur Stefansson
    Department of Vascular Biology, J H Holland Laboratory, Rockville, Maryland 20855, USA
    J Biol Chem 279:29981-7. 2004
  8. pmc Structure of human neutrophil elastase in complex with a peptide chloromethyl ketone inhibitor at 1.84-A resolution
    M A Navia
    Merck Sharp and Dohme Research Laboratories, Rahway, NJ 07065
    Proc Natl Acad Sci U S A 86:7-11. 1989
  9. ncbi Overexpression of mucin genes induced by interleukin-1 beta, tumor necrosis factor-alpha, lipopolysaccharide, and neutrophil elastase is inhibited by a retinoic acid receptor alpha antagonist
    Ja Seok Koo
    Department of Thoracic Head and Neck Medical Oncology, The University of Texas M D Anderson Cancer Center, Houston, TX 77030, USA
    Exp Lung Res 28:315-32. 2002
  10. ncbi Molecular cloning of human neutrophil elastase
    D Farley
    Department of Biochemistry, University of Georgia, Athens
    Biol Chem Hoppe Seyler 369:3-7. 1988

Research Grants

  1. ARUNI BHATNAGAR BHATNAGAR; Fiscal Year: 2015
  2. Lipid peroxidation product induced DNA damage in the p53 gene and liver cancer
    ERIC MOON SHONG M TANG; Fiscal Year: 2010
  3. Role and Molecular Mechanisms of Corneal Aldehyde Dehydrogenase
    Vasilis Vasiliou; Fiscal Year: 2013
  4. Cardioprotective Mechanisms of Glutathione S-transferase P
    DANIEL JOSEPH CONKLIN; Fiscal Year: 2012
  5. Detoxification of Xenobiotics in Ocular Tissue
    YOGESH CHANDRA AWASTHI; Fiscal Year: 2010
  6. RICHARD MICHAEL LOPACHIN; Fiscal Year: 2015
  7. Zhanxiang Zhou; Fiscal Year: 2014
  8. Aldose Reductase: A Regulator of Inflammatory Signals
    KOTA VENKATA RAMANA; Fiscal Year: 2011
  9. LIPID PEROXIDATION\CELL SIGNALING-CC14-INDUCED FIBROSIS
    DENNIS PETERSEN; Fiscal Year: 2004
  10. Modification of PDI by 4-HNE and 4-ONE and its Role in Ethanol-Induced ER Stress
    James J Galligan; Fiscal Year: 2011

Detail Information

Publications324 found, 100 shown here

  1. ncbi HIV-1 preferentially binds receptors copatched with cell-surface elastase
    Cynthia L Bristow
    Laboratory of Cellular Physiology and Immunology, The Rockefeller University and Population Council, New York, NY 10021, USA
    Blood 102:4479-86. 2003
    Human leukocyte elastase (HLE) interacts with HIV-1 glycoprotein (gp)41, suggesting a nonenzymatic receptor function for HLE in the context of HIV-1...
  2. ncbi G-CSF induces stem cell mobilization by decreasing bone marrow SDF-1 and up-regulating CXCR4
    Isabelle Petit
    Department of Immunology, The Weizmann Institute of Science, Rehovot, Israel
    Nat Immunol 3:687-94. 2002
    ..The gradual decrease of BM SDF-1, due mostly to its degradation by neutrophil elastase, correlated with stem cell mobilization. Elastase inhibition reduced both activities...
  3. ncbi Modulation of tissue factor and tissue factor pathway inhibitor-1 by neutrophil proteases
    Birgit A Steppich
    Deutsches Herzzentrum der Technischen Universität München, Lazarettstr 36, 80636 München, Germany
    Thromb Haemost 100:1068-75. 2008
    ..The effects of elastase (HNE), cathepsin G (CG), and proteinase 3 (PR3) on expression of tissue factor (TF) and tissue factor pathway inhibitor-..
  4. pmc Is lysyl oxidase-like protein-1, alpha-1 antitrypsin, and neutrophil elastase site specific in pelvic organ prolapse?
    Weng Chi Man
    Department of Obstetrics and Gynecology, Stanford University School of Medicine, 300 Pasteur Drive, HH 333, Stanford, CA 94305 5317, USA
    Int Urogynecol J Pelvic Floor Dysfunct 20:1423-9. 2009
    We investigated whether the expression of alpha-1 antitrypsin (ATT), neutrophil elastase (NE), and lysyl oxidase-like protein 1 (LOXL-1) vary within the vagina in subjects with pelvic organ prolapse (POP).
  5. pmc Mechanism of heparin acceleration of tissue inhibitor of metalloproteases-1 (TIMP-1) degradation by the human neutrophil elastase
    Gabriel L C Nunes
    Centro Interdisciplinar de Investigação Bioquímica, Universidade de Mogi das Cruzes, Mogi das Cruzes, Brazil
    PLoS ONE 6:e21525. 2011
    Heparin has been shown to regulate human neutrophil elastase (HNE) activity...
  6. doi Exploring Tagetes erecta Linn flower for the elastase, hyaluronidase and MMP-1 inhibitory activity
    Niladri Maity
    School of Natural Product Studies, Department of Pharmaceutical Technology, Jadavpur University, Kolkata 700032, India
    J Ethnopharmacol 137:1300-5. 2011
    ..The aim of the present experiment was to evaluate the anti-wrinkle potential of standardized flower extract of Tagetes erecta...
  7. ncbi Mutants of plasminogen activator inhibitor-1 designed to inhibit neutrophil elastase and cathepsin G are more effective in vivo than their endogenous inhibitors
    Steingrimur Stefansson
    Department of Vascular Biology, J H Holland Laboratory, Rockville, Maryland 20855, USA
    J Biol Chem 279:29981-7. 2004
    b>Neutrophil elastase and cathepsin G are abundant intracellular neutrophil proteinases that have an important role in destroying ingested particles...
  8. pmc Structure of human neutrophil elastase in complex with a peptide chloromethyl ketone inhibitor at 1.84-A resolution
    M A Navia
    Merck Sharp and Dohme Research Laboratories, Rahway, NJ 07065
    Proc Natl Acad Sci U S A 86:7-11. 1989
    Human neutrophil elastase (HNE) has been implicated as a major contributor to tissue destruction in various disease states, including emphysema...
  9. ncbi Overexpression of mucin genes induced by interleukin-1 beta, tumor necrosis factor-alpha, lipopolysaccharide, and neutrophil elastase is inhibited by a retinoic acid receptor alpha antagonist
    Ja Seok Koo
    Department of Thoracic Head and Neck Medical Oncology, The University of Texas M D Anderson Cancer Center, Houston, TX 77030, USA
    Exp Lung Res 28:315-32. 2002
    Proinflammatory cytokines, lipopolysaccharide (LPS), and neutrophil elastase (NE) have been implicated in the induction of hypersecretion of respiratory mucus...
  10. ncbi Molecular cloning of human neutrophil elastase
    D Farley
    Department of Biochemistry, University of Georgia, Athens
    Biol Chem Hoppe Seyler 369:3-7. 1988
    The human U937 cell line, a promonocyte-like leukemic line, has been shown to synthesize neutrophil elastase (Senior, R. M. et al. (1982) J. Clin. Invest. 69, 384-393)...
  11. ncbi Syndecans, heparan sulfate proteoglycans, maintain the proteolytic balance of acute wound fluids
    V Kainulainen
    Division of Developmental and Newborn Biology, Department of Pediatrics, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Biol Chem 273:11563-9. 1998
    ..Thus, syndecans are shed into acute wound fluids, where they can modify the proteolytic balance of the fluid. This suggests a novel physiological role for these soluble heparan sulfate proteoglycans...
  12. ncbi Mutations in ELA2, encoding neutrophil elastase, define a 21-day biological clock in cyclic haematopoiesis
    M Horwitz
    Division of Medical Genetics, University of Washington School of Medicine, Seattle, Washington, USA
    Nat Genet 23:433-6. 1999
    ..3. We identified 7 different single-base substitutions in the gene (ELA2) encoding neutrophil elastase (EC 3. 4.21...
  13. ncbi Mutations in the ELA2 gene encoding neutrophil elastase are present in most patients with sporadic severe congenital neutropenia but only in some patients with the familial form of the disease
    P J Ancliff
    Department of Haematology, University College London, United Kingdom
    Blood 98:2645-50. 2001
    ..Heterozygous mutations in the ELA2 gene encoding neutrophil elastase were detected in all families studied...
  14. ncbi Killing activity of neutrophils is mediated through activation of proteases by K+ flux
    Emer P Reeves
    Centre for Molecular Medicine, University College London, 5 University Street, London WC1E 6JJ, UK
    Nature 416:291-7. 2002
    ..We show that it is the proteases, thus activated, that are primarily responsible for the destruction of the bacteria...
  15. ncbi Mutations in the ELA2 gene correlate with more severe expression of neutropenia: a study of 81 patients from the French Neutropenia Register
    Christine Bellanne-Chantelot
    Hopital Saint Antoine, Laboratoire de d Embryologie Pathologique et de Cytogénétique, 184 rue du Fbg Saint Antoine, 75012 Paris, France
    Blood 103:4119-25. 2004
    Heterozygous mutations of the gene encoding neutrophil elastase (ELA2) have been associated with cyclic neutropenia (CN) and severe congenital neutropenia (SCN)...
  16. pmc Neutrophil elastase mutations and risk of leukaemia in severe congenital neutropenia
    Philip S Rosenberg
    Biostatistics Branch, Division of Cancer Epidemiology and Genetics, Department of Health and Human Services, National Cancer Institute, National Institutes of Health, Rockville, MD, USA
    Br J Haematol 140:210-3. 2008
    ..Registry who were on long-term treatment with granulocyte colony-stimulating factor and for whom the neutrophil elastase (ELA2) gene was sequenced...
  17. pmc Plasma neutrophil elastase and elafin imbalance is associated with acute respiratory distress syndrome (ARDS) development
    Zhaoxi Wang
    Department of Environmental Health, Harvard School of Public Health, Boston, MA, USA
    PLoS ONE 4:e4380. 2009
    ..an exploratory study of genome-wide gene expression in whole blood and found that the expression of neutrophil elastase inhibitor (PI3, elafin) was down-regulated during the early phase of ARDS...
  18. pmc Prevalence of mutations in ELANE, GFI1, HAX1, SBDS, WAS and G6PC3 in patients with severe congenital neutropenia
    Jun Xia
    Division of Oncology, Department of Medicine, Washington University School of Medicine, St Louis, MO 63110, USA
    Br J Haematol 147:535-42. 2009
    Severe congenital neutropenia (SCN) is a genetically heterogeneous syndrome associated with mutations of ELANE (ELA2), HAX1, GFI1, WAS, CSF3R or G6PC3...
  19. pmc Human neutrophil elastase-mediated cleavage sites of MMP-9 and TIMP-1: implications to cystic fibrosis proteolytic dysfunction
    Patricia L Jackson
    Department of Medicine, University of Alabama at Birmingham, Birmingham, Alabama, USA
    Mol Med 16:159-66. 2010
    ..We demonstrated increased gelatinolytic activity in CF sputum. These samples had elevated human neutrophil elastase (HNE) levels which correlated with an increased MMP-9/TIMP-1 ratio...
  20. pmc Cyclic neutropenia and severe congenital neutropenia in patients with a shared ELANE mutation and paternal haplotype: evidence for phenotype determination by modifying genes
    Peter E Newburger
    Department of Pediatrics, University of Massachusetts Medical School, Worcester, Massachusetts 01655, USA
    Pediatr Blood Cancer 55:314-7. 2010
    ..Mutations of the ELANE gene (the symbol recently replacing ELA2) are considered largely responsible for most cases of CN and SCN, but ..
  21. pmc Neutrophil elastase and myeloperoxidase regulate the formation of neutrophil extracellular traps
    Venizelos Papayannopoulos
    Department of Cellular Microbiology, Max Planck Institute for Infection Biology, Berlin, Germany
    J Cell Biol 191:677-91. 2010
    ..We show that upon activation, neutrophil elastase (NE) escapes from azurophilic granules and translocates to the nucleus, where it partially degrades ..
  22. doi The spectrum of ELANE mutations and their implications in severe congenital and cyclic neutropenia
    Manuela Germeshausen
    Department of Pediatric Hematology and Oncology, Hannover Medical School, Hannover, Germany
    Hum Mutat 34:905-14. 2013
    b>Neutrophil elastase gene (ELANE) mutations are responsible for the majority of cases of severe congenital neutropenia (CN) and cyclic neutropenia (CyN)...
  23. ncbi Interaction of heparin cofactor II with neutrophil elastase and cathepsin G
    C W Pratt
    Department of Pathology, University of North Carolina School of Medicine, Chapel Hill 27599 7035
    J Biol Chem 265:6092-7. 1990
    We investigated the interaction of the human plasma proteinase inhibitor heparin cofactor II (HC) with human neutrophil elastase and cathepsin G in order to examine 1) proteinase inhibition by HC, 2) inactivation of HC, and 3) the effect ..
  24. pmc Expression of the neutrophil elastase gene during human bone marrow cell differentiation
    P Fouret
    Pulmonary Branch, National Heart, Lung, and Blood Institute, Bethesda, Maryland 20892
    J Exp Med 169:833-45. 1989
    b>Neutrophil elastase, a potent serine protease carried and released by activated neutrophils, is not synthesized by neutrophils, but by their bone marrow precursor cells...
  25. ncbi Myelomonocytic cell lineage expression of the neutrophil elastase gene
    H Takahashi
    Pulmonary Branch, National Heart, Lung and Blood Institute, Bethesda, Maryland 20892
    J Biol Chem 263:2543-7. 1988
    Human neutrophil elastase (NE) functions as a powerful serine protease capable of attacking a broad range of proteins. To examine the cellular site(s) of NE gene expression, a 0...
  26. ncbi Mutations in the gene encoding neutrophil elastase in congenital and cyclic neutropenia
    D C Dale
    Divisions of Hematology and Medical Genetics, Department of Medicine and the Markey Molecular Medicine Center, University of Washington School of Medicine, Seattle, WA, USA
    Blood 96:2317-22. 2000
    ..3, and this disease is now attributable to mutations of the gene encoding neutrophil elastase (the ELA2 gene)...
  27. ncbi Paternal mosaicism proves the pathogenic nature of mutations in neutrophil elastase in severe congenital neutropenia
    Phil J Ancliff
    Department of Haematology and Immunology, University College London and Great Ormond Street Children s Hospital, London, United Kingdom
    Blood 100:707-9. 2002
    Heterozygous mutations in neutrophil elastase have been detected in many sporadic cases of congenital neutropenia...
  28. pmc Mutations in proto-oncogene GFI1 cause human neutropenia and target ELA2
    Richard E Person
    Department of Pathology, University of Washington School of Medicine, Box 357720, Seattle, Washington 98195, USA
    Nat Genet 34:308-12. 2003
    ..as a candidate for association with neutropenia in affected individuals without mutations in ELA2 (encoding neutrophil elastase), the most common cause of severe congenital neutropenia (SCN; ref. 3)...
  29. ncbi A comparison of the defective granulopoiesis in childhood cyclic neutropenia and in severe congenital neutropenia
    Yasuhiko Sera
    Department of Pediatrics, Hiroshima University Graduate School of Biomedical Sciences, Hiroshima, 1 2 3 Kasumi, Minami Ku, Hiroshima 734 8551, Japan
    Haematologica 90:1032-41. 2005
    ..Mutations in the neutrophil elastase gene, ELA2, have been reported in patients with CyN and in those with SCN...
  30. ncbi Elevated plasma levels of fibrin degradation products by granulocyte-derived elastase in patients with disseminated intravascular coagulation
    Takeshi Matsumoto
    Second Department of Internal Medicine, Mie University School of Medicine, Tsu, Japan
    Clin Appl Thromb Hemost 11:391-400. 2005
    ..These results suggest that GE-XDP is a potentially useful marker for the diagnosis of overt-DIC and as a predictor of organ failure-related outcome...
  31. ncbi Mutations in neutrophil elastase causing congenital neutropenia lead to cytoplasmic protein accumulation and induction of the unfolded protein response
    Inga Köllner
    Institute of Cell and Molecular Pathology, The Institute of Virology, the Department of Pediatric Hematology and Oncology, Hannover Medical School, Germany
    Blood 108:493-500. 2006
    ..Heterozygous mutations in the gene encoding neutrophil elastase (ELA2) have been reported in both diseases...
  32. ncbi Influence of charge distribution at the active site surface on the substrate specificity of human neutrophil protease 3 and elastase. A kinetic and molecular modeling analysis
    Brice Korkmaz
    INSERM U618, Faculty of Medicine, 10 Bd Tonnellé, 37032 Tours Cedex, France
    J Biol Chem 282:1989-97. 2007
    The biological functions of human neutrophil protease 3 (Pr3) differ from those of neutrophil elastase despite their close structural and functional resemblance...
  33. ncbi HAX1 deficiency causes autosomal recessive severe congenital neutropenia (Kostmann disease)
    Christoph Klein
    Department of Pediatric Hematology Oncology, Hannover Medical School, Carl Neuberg Strasse 1, 30625 Hannover, Germany
    Nat Genet 39:86-92. 2007
    ..Our findings suggest that HAX1 is a major regulator of myeloid homeostasis and underline the significance of genetic control of apoptosis in neutrophil development...
  34. ncbi Double de novo mutations of ELA2 in cyclic and severe congenital neutropenia
    Stephen J Salipante
    Department of Genome Sciences, University of Washington, Seattle, Washington 98195, USA
    Hum Mutat 28:874-81. 2007
    Heterozygous mutations of ELA2, encoding the protease neutrophil elastase (NE), cause either autosomal dominant cyclic neutropenia or severe congenital neutropenia (SCN)...
  35. pmc Mutations of the ELA2 gene found in patients with severe congenital neutropenia induce the unfolded protein response and cellular apoptosis
    David S Grenda
    Division of Oncology, Washington University School of Medicine, St Louis, MO 63110, USA
    Blood 110:4179-87. 2007
    ..Mutations of the ELA2 gene encoding neutrophil elastase (NE) are responsible for most cases of SCN and cyclic neutropenia (CN), a related but milder disorder of ..
  36. doi Shed syndecan-1 restricts neutrophil elastase from alpha1-antitrypsin in neutrophilic airway inflammation
    Stanley C H Chan
    Department of Biochemistry, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, SAR, China
    Am J Respir Cell Mol Biol 41:620-8. 2009
    Persistent proteolytic imbalance in chronic inflammatory diseases has been ascribed to neutrophil elastase (NE)/antielastase imbalance in wound fluids...
  37. doi Ela2 mutations and clinical manifestations in familial congenital neutropenia
    Masaaki Shiohara
    Department of Pediatrics, Shinshu University School of Medicine, Matsumoto, Japan
    J Pediatr Hematol Oncol 31:319-24. 2009
    ..neutropenia (SCN) and cyclic neutropenia (CN) in addition to 3 sporadic cases of SCN were analyzed for neutrophil elastase (Ela2) gene mutation...
  38. doi Neutrophil elastase is severely down-regulated in severe congenital neutropenia independent of ELA2 or HAX1 mutations but dependent on LEF-1
    Julia Skokowa
    Department of Molecular Hematopoiesis, Hannover Medical School, Hannover, Germany
    Blood 114:3044-51. 2009
    ..present study we demonstrated that ELA2 mRNA expression in myeloid progenitors and plasma protein levels of neutrophil elastase (NE) were markedly reduced in patients with CN harboring mutations in either ELA2 or HAX-1 genes...
  39. ncbi Vascular cell adhesion molecule-1 (CD106) is cleaved by neutrophil proteases in the bone marrow following hematopoietic progenitor cell mobilization by granulocyte colony-stimulating factor
    J P Levesque
    Peter MacCallum Cancer Institute, East Melbourne, Victoria, Australia
    Blood 98:1289-97. 2001
    ..Two serine proteases, namely, neutrophil elastase and cathepsin G, were identified, which cleave VCAM-1 and are released by neutrophils accumulating in the ..
  40. pmc Leukocyte elastase induces lung epithelial apoptosis via a PAR-1-, NF-kappaB-, and p53-dependent pathway
    Tomoko Suzuki
    Division of Respirology, Department of Medicine, University of Toronto and Toronto General Hospital Research Institute of the University Health Network, Toronto, Ontario, Canada
    Am J Respir Cell Mol Biol 41:742-55. 2009
    b>Leukocyte elastase induces apoptosis of lung epithelial cells via alterations in mitochondrial permeability, but the signaling pathways regulating this response remain uncertain...
  41. ncbi The Bowman-Birk inhibitor reactive site loop sequence represents an independent structural beta-hairpin motif
    A B Brauer
    Department of Chemistry, Imperial College of Science Technology and Medicine, South Kensington, London, UK
    J Mol Biol 306:799-807. 2001
    ..sequence analysed originates from a previously reported study, which identified potent inhibitors of human leukocyte elastase from screening a combinatorial peptide library based on the short protein beta-sheet segment that forms the ..
  42. ncbi Simultaneous determination of Pseudomonas aeruginosa elastase, human leukocyte elastase and cathepsin G activities by micellar electrokinetic chromatography
    S Viglio
    Dipartimento di Biochimica A. Castellani, , Italy
    J Chromatogr A 846:125-34. 1999
    ..that MEKC differentiates between the enzymatic activities of Pseudomonas aeruginosa elastase (PsE) and human leukocyte elastase (HLE) or cathepsin G (Cat G) in assays using the chromogenic peptide substrates Suc-Ala-Ala-Ala-NA or Suc-..
  43. ncbi 6-Acylamino-2-1(ethylsulfonyl)oxy]-1H-isoindole-1,3-diones mechanism-based inhibitors of human leukocyte elastase and cathepsin G: effect of chirality in the 6-acylamino substituent on inhibitory potency and selectivity
    L M Vagnoni
    Department of Pharmaceutical Chemistry, College of Pharmacy, Rutgers University, Piscataway, NJ 08854, USA
    Bioorg Med Chem 9:637-45. 2001
    Inhibition of human leukocyte elastase(HLE) by a series of 6-acylamino-2-[(ethylsulfonyl)oxy)]-1H-isoindole-1,3-diones was determined and compared to their inhibition of ChT, PPE, and Cat G...
  44. ncbi Coumarinic derivatives as mechanism-based inhibitors of alpha-chymotrypsin and human leukocyte elastase
    L Pochet
    Department of Pharmacy, University of Namur, FUNDP, Belgium
    Bioorg Med Chem 8:1489-501. 2000
    Novel coumarinic derivatives were synthesized and tested for their inhibitory potency toward alpha-CT and HLE. Cycloalkyl esters and amides were found to be essentially inactive on both enzymes...
  45. ncbi Neutral proteinases in human intervertebral disc. Role in degeneration and probable origin
    K Fujita
    Department of Orthopaedic Surgery, Urawa City Hospital, Saitama, Japan
    Spine (Phila Pa 1976) 18:1766-73. 1993
    ..Furthermore, they were considered to be leukocyte elastases because anti-leukocyte elastase antibody inhibited the proteinase activity of these enzymes...
  46. ncbi 6-Acylamino-2-[(alkylsulfonyl)oxy]-1H-isoindole-1,3-dione mechanism-based inhibitors of human leukocyte elastase
    J E Kerrigan
    Department of Chemistry, University of South Alabama, Mobile 36688, USA
    Bioorg Med Chem Lett 10:27-30. 2000
    ..2-[(alkylsulfonyl)oxy]-6-substituted-1H-isoindole-1,3-diones' inhibition of chymotrypsin compared to inhibition of HLE reveals that acylamino substitution in the 6-position increases selectivity and potency of these inhibitors for HLE...
  47. doi Inhibition of human leukocyte elastase, plasmin and matrix metalloproteinases by oleic acid and oleoyl-galardin derivative(s)
    Gautier Moroy
    Universite Paris Diderot, Molécules Thérapeutiques in silico, INSERM UMR S 973, Bât Lamarck, 35 rue Helene Brion, 75205 Paris Cedex 13, France
    Biochem Pharmacol 81:626-35. 2011
    Molecular modeling was undertaken at aims to analyze the interactions between oleic acid and human leukocyte elastase (HLE), plasmin and matrix metalloproteinase-2 (MMP-2), involved in the inhibitory capacity of fatty acid towards those ..
  48. ncbi Specific inhibition of thrombin-induced cell activation by the neutrophil proteinases elastase, cathepsin G, and proteinase 3: evidence for distinct cleavage sites within the aminoterminal domain of the thrombin receptor
    P Renesto
    Unite de Pharmacologie Cellulaire, Institut Pasteur, Paris, France
    Blood 89:1944-53. 1997
    The aim of this study was to investigate the inhibitory effects of human leukocyte elastase (HLE), cathepsin G (Cat G), and proteinase 3 (PR3) on the activation of endothelial cells (ECs) and platelets by thrombin and to elucidate the ..
  49. ncbi [The effect of leukocyte elastase on high molecular weight kininogen from human plasma in the presence of alpha-1 protease inhibitor. Analysis of proteolytic degradation]
    V L Dotsenko
    Russian Medical Academy for Postgraduate Education, Barrikadnaya 2, Moscow 123836, Russia
    Vopr Med Khim 47:55-71. 2001
    Degranulation of polymorphonuclear leukocytes (neutrophils) and releasing of leukocyte elastase during inflammation occur not only in injured tissue but in plasma in the presence of considerable excess of alpha-1 proteinase inhibitor (..
  50. ncbi The prognostic value of polymorphonuclear leukocyte elastase in patients with primary breast cancer
    John A Foekens
    Erasmus MC Daniel den Hoed, Department of Medical Oncology, 3000 DR Rotterdam, The Netherlands
    Cancer Res 63:337-41. 2003
    ..of serine proteases, including urokinase-type plasminogen activator (uPA), plasmin,and polymorphonuclear leukocyte elastase (PMN-E), have been implicated in the processes of tumor cell invasion and metastasis...
  51. ncbi Neutrophil serine proteinases activate human nonepithelial cells to produce inflammatory cytokines through protease-activated receptor 2
    Akiko Uehara
    Department of Microbiology and Immunology, Graduate School of Dentistry, Tohoku University, Sendai, Japan
    J Immunol 170:5690-6. 2003
    ..The present study examined whether other neutrophil serine proteinases, human leukocyte elastase (HLE), and cathepsin G (Cat G) activate nonepithelial cells, human gingival fibroblasts (HGF)...
  52. ncbi DNA from bronchial secretions modulates elastase inhibition by alpha(1)-proteinase inhibitor and oxidized secretory leukoprotease inhibitor
    Q L Ying
    Department of Pathology, State University of New York at Stony Brook, Stony Brook, New York 11794, USA
    Am J Respir Cell Mol Biol 23:506-13. 2000
    Previously we reported that DNA from sputum promotes the inhibition of human leukocyte elastase (HLE) by native secretory leukoprotease inhibitor (SLPI)...
  53. pmc Inhibition by recombinant SLPI and half-SLPI (Asn55-Ala107) of elastase and cathepsin G activities: consequence for neutrophil-platelet cooperation
    P Renesto
    , , Institut Pasteur, Paris, France
    Br J Pharmacol 108:1100-6. 1993
    1. The capacity of recombinant human secretory leukocyte proteinase inhibitor (SLPI) to inhibit human leukocyte elastase (HLE) and cathepsin G (Cat G) was investigated and compared with a recombinant truncated form (carboxyl-terminal ..
  54. ncbi The N-terminal tetrapeptide of neutrophil proteinase 3 causes S-phase arrest in granulopoietic progenitors
    Stefan Sköld
    Division of Hematology and Transfusion Medicine, Department of Laboratory Medicine, Biomedical Center, Lund University, Lund, Sweden
    Exp Hematol 33:1329-36. 2005
    ..H, K, and M are able to reduce the fraction of granulopoietic progenitors (CFU-GM) in S-phase, whereas human leukocyte elastase (HLE) and cathepsin G lack this ability...
  55. ncbi The sulfate groups of chondroitin sulfate- and heparan sulfate-containing proteoglycans in neutrophil plasma membranes are novel binding sites for human leukocyte elastase and cathepsin G
    Edward J Campbell
    Division of Pulmonary and Critical Care Medicine, Brigham and Women s Hospital, Harvard Medical School, Boston, MA 02115, USA
    J Biol Chem 282:14645-54. 2007
    Human leukocyte elastase (HLE) and cathepsin G (CG) are expressed at high levels on the surface of activated human neutrophils (PMN) in catalytically active but inhibitor-resistant forms having the potential to contribute to tissue injury...
  56. ncbi Cleavage of CD14 on human gingival fibroblasts cocultured with activated neutrophils is mediated by human leukocyte elastase resulting in down-regulation of lipopolysaccharide-induced IL-8 production
    E Nemoto
    Division of Periodontics and Endodontics, Tohoku University Graduate School of Dentistry, Sendai, Japan
    J Immunol 165:5807-13. 2000
    ..A protease responsible for the reduction was found to be human leukocyte elastase (HLE) secreted from the activated PMNs by use of various protease inhibitors, although HLE was only ..
  57. pmc Elevated expression of polymorphonuclear leukocyte elastase in breast cancer tissue is associated with tamoxifen failure in patients with advanced disease
    J A Foekens
    Department of Medical Oncology, Erasmus MC Daniel den Hoed, Rotterdam, The Netherlands
    Br J Cancer 88:1084-90. 2003
    Besides a variety of other proteases, polymorphonuclear leukocyte elastase (PMN-E) is also suggested to play a role in the processes of tumour cell invasion and metastasis...
  58. ncbi Leukocyte elastase inhibition therapy in cystic fibrosis: role of glycosylation on the distribution of alpha-1-proteinase inhibitor in blood versus lung
    André M Cantin
    Pulmonary Research Unit, Faculty of Medicine, University of Sherbrooke, 3110 12th Avenue N, Fleurimont, Quebec J1H 5N4, Canada
    J Aerosol Med 15:141-8. 2002
    ..Airway infiltration by neutrophils will then lead to an increase in human leukocyte elastase (HLE) within the extracellular compartment, thereby producing deleterious effects...
  59. ncbi Biochemical and pharmacological characterization of 2-(9-(2-piperidinoethoxy)-4-oxo-4H-pyrido[1,2-a]pyrimidin-2-yloxymethyl)-4-(1-methylethyl)-6-methoxy-1,2-benzisothiazol-3(2H)-one-1,1-dioxide (SSR69071), a novel, orally active elastase inhibitor
    Zoltan Kapui
    Discovery Research, Sanofi Synthelabo Internal Medicine Budapest Site, Chinoin, Sanofi Synthelabo Group, Budapest, Hungary
    J Pharmacol Exp Ther 305:451-9. 2003
    Human leukocyte elastase (HLE) is a proteinase capable of degrading a variety of proteins. Under normal circumstances, the proteolytic activity of HLE is effectively controlled by its natural inhibitors...
  60. ncbi Generation of globular fragment of adiponectin by leukocyte elastase secreted by monocytic cell line THP-1
    Hironori Waki
    Department of Metabolic Diseases, Graduate School of Medicine, University of Tokyo, 7 3 1 Hongo, Bunkyo, Tokyo 113 8655, Japan
    Endocrinology 146:790-6. 2005
    ..The cleavage was thought to be mediated by leukocyte elastase (also known as neutrophil elastase) based on the following observations...
  61. ncbi Kinetics of cytokines and PMN-E in thoracoscopic esophagectomy
    T Fukunaga
    Department of Surgery, Urayasu Hospital of Juntendo University, School of Medicine, 2 1 1, Tomioka, Urayasu shi, Chiba 279 0021, Japan
    Surg Endosc 15:1484-7. 2001
    Perioperative increases in the levels of cytokines and polymorphonuclear leukocyte elastase (PMN-E) have been shown to be related to degree of surgical trauma.
  62. ncbi Design, synthesis, and enzymatic evaluation of N1-acyloxyalkyl- and N1-oxazolidin-2,4-dion-5-yl-substituted beta-lactams as novel inhibitors of human leukocyte elastase
    Rui Moreira
    CECF, Faculdade de Farmacia, Universidade de Lisboa, Av Forcas Armadas, 1600 083 Lisboa, Portugal
    J Med Chem 48:4861-70. 2005
    Human leukocyte elastase (HLE) is a serine protease that very efficiently degrades various tissue matrix proteins such as elastin...
  63. ncbi Proteolysis of factor V by cathepsin G and elastase indicates that cleavage at Arg1545 optimizes cofactor function by facilitating factor Xa binding
    R M Camire
    Department of Biochemistry, University of Vermont, College of Medicine, Burlington, Vermont 05405, USA
    Biochemistry 37:11896-906. 1998
    ..releasing peptides of approximately 2000 and approximately 3000 Da, respectively, generating FVaIIa/CG and FVaIIa/HNE. The functional activity of each cofactor species was assessed either by clotting assay or by employing a purified ..
  64. ncbi [Effects of erythromycin on the synthesis of interleukin-8 and gamma-glutamylcysteine synthetase induced by 4-hydroxynonenal in the bronchial epithelial cells]
    Liang Yu
    Guangzhou Institute of Respiratory Disease, The First Affiliated Hospital of Guangzhou Medical College, Guangzhou 510182, China
    Zhonghua Jie He He Hu Xi Za Zhi 32:133-8. 2009
    This study was to explore the role of erythromycin on the synthesis of interleukin-8 (IL-8) and gamma-GCS treated by 4-hydroxynonenal (4-HNE) in bronchial epithelial cells (16-HBE).
  65. ncbi Generation of endostatin by matrix metalloproteinase and cathepsin from human limbocorneal epithelial cells cultivated on amniotic membrane
    David Hui Kang Ma
    Limbal Stem Cell Laboratory, Department of Ophthalmology, Chang Gung Memorial Hospital, Kweishan Township, Taoyuan County 333, Taiwan
    Invest Ophthalmol Vis Sci 48:644-51. 2007
    Cultured human limbocorneal epithelial (HLE) cells secrete endostatin-related molecules that are augmented when the cells are cultivated on denuded amniotic membrane (DAM)...
  66. ncbi Formation of trans-4-hydroxy-2-nonenal- and other enal-derived cyclic DNA adducts from omega-3 and omega-6 polyunsaturated fatty acids and their roles in DNA repair and human p53 gene mutation
    Fung Lung Chung
    Division of Carcinogenesis and Molecular Epidemiology, Institute for Cancer Prevention, American Health Foundation Cancer Center, Valhalla, NY 10595, USA
    Mutat Res 531:25-36. 2003
    ..aldehydes or enals, including acrolein (Acr), crotonaldehyde (Cro), and trans-4-hydroxy-2-nonenal (HNE), have been detected as endogenous DNA lesions in rodent and human tissues...
  67. ncbi Endogenous glutathione adducts
    Ian A Blair
    Center for Cancer Pharmacology and Institute for Translational Medicine and Therapeutics, University of Pennsylvania School of Medicine, Philadelphia, PA 19104 6160, USA
    Curr Drug Metab 7:853-72. 2006
    ..GSH-adducts derived from eicosanoids, isoprostanes, estrogens, catecholamines, and 4-hydroxy-2(E)-nonenal (HNE)...
  68. ncbi Polymorphonuclear elastase as a diagnostic marker of acute pyelonephritis in children
    A Fretzayas
    P and A Kyriakou Children s Hospital, Second Department of Pediatrics, University of Athens, Thibon and Levadias St, Goudi, Athens 115 27, Greece
    Pediatrics 105:E28. 2000
    ..The aim of this study was to investigate the diagnostic value of polymorphonuclear elastase-a(1)-antitrypsin complex (E-a(1)-Pi) for the detection of acute pyelonephritis in children.
  69. ncbi Lipoperoxidation in hepatic subcellular compartments of diabetic rats
    N Traverso
    Department of Experimental Medicine, University of Genova, Italy
    Free Radic Biol Med 26:538-47. 1999
    It is known that an accumulation of lipoperoxidative aldehydes malondialdehyde (MDA) and 4-hydroxynonenal (HNE) takes place in liver mitochondria during aging...
  70. ncbi Sensory nerves in central and peripheral control of pancreatic integrity by leptin and melatonin
    J Jaworek
    Department of Physiology, University School of Medicine, Jagiellonian University, Krakow, Poland
    J Physiol Pharmacol 53:51-74. 2002
    ..afforded by leptin or melatonin, 3/ to assess the effect of tested peptides on lipid peroxidation products (MDA + 4-HNE) in the pancreas of CIP rats, 4/ to investigate the influence of leptin or melatonin on nitric oxide (NO) release ..
  71. ncbi Localization of lipid peroxidation-derived protein epitopes in the porcine corpus luteum
    J D Brannian
    Department of Obstetrics and Gynecology, University of South Dakota School of Medicine, Sioux Falls 57105 1570, USA
    Biol Reprod 57:1461-6. 1997
    ..by ROS, leading to the formation of toxic aldehydes such as malondialdehyde (MDA) and 4-hydroxy-nonenal (4-HNE) that can attack cellular and extracellular proteins...
  72. ncbi Detoxification of 4-hydroxynonenal (HNE) in keratinocytes: characterization of conjugated metabolites by liquid chromatography/electrospray ionization tandem mass spectrometry
    Giancarlo Aldini
    Istituto Chimico Farmaceutico Tossicologico, University of Milan, Viale Abruzzi 42, 20131 Milan, Italy
    J Mass Spectrom 38:1160-8. 2003
    ..in the skin following UV exposure, among which the most abundant and toxic product is 4-hydroxy-trans-2,3-nonenal (HNE)...
  73. ncbi Noninvasive markers in the assessment of intestinal inflammation in inflammatory bowel diseases: performance of fecal lactoferrin, calprotectin, and PMN-elastase, CRP, and clinical indices
    Jost Langhorst
    Department of Internal and Integrative Medicine, Kliniken Essen Mitte, University of Duisburg Essen, Germany
    Am J Gastroenterol 103:162-9. 2008
    ..this study was to compare the performance of fecal lactoferrin (Lf), calprotectin (Cal), polymorphonuclear neutrophil elastase (PMN-e), as well as serum C-reactive protein (CRP) in patients with inflammatory bowel diseases (IBD) to ..
  74. ncbi Evidence that amyloid beta-peptide-induced lipid peroxidation and its sequelae in Alzheimer's disease brain contribute to neuronal death
    D Allan Butterfield
    Department of Chemistry, Center of Membrane Sciences, Sanders Brown Center on Aging, University of Kentucky, Lexington, KY 40506 0055, USA
    Neurobiol Aging 23:655-64. 2002
    ..Two reactive products of lipid peroxidation are the alkenals, 4-hydroxynonenal (HNE) and 2-propenal (acrolein)...
  75. ncbi Detoxification of cytotoxic alpha,beta-unsaturated aldehydes by carnosine: characterization of conjugated adducts by electrospray ionization tandem mass spectrometry and detection by liquid chromatography/mass spectrometry in rat skeletal muscle
    Giancarlo Aldini
    Istituto Chimico Farmaceutico Tossicologico, University of Milan, Viale Abruzzi 42, 20131 Milan, Italy
    J Mass Spectrom 37:1219-28. 2002
    ..are further degraded to several products, among which unsaturated aldehydes such as 4-hydroxy-trans-2-nonenal (HNE) play an important role in mediating the pathological effects of oxidative stress...
  76. pmc Chemistry and biology of DNA containing 1,N(2)-deoxyguanosine adducts of the alpha,beta-unsaturated aldehydes acrolein, crotonaldehyde, and 4-hydroxynonenal
    Irina G Minko
    Center for Research on Occupational and Environmental Toxicology, Oregon Health and Science University, Portland, Oregon 97239, USA
    Chem Res Toxicol 22:759-78. 2009
    The alpha,beta-unsaturated aldehydes (enals) acrolein, crotonaldehyde, and trans-4-hydroxynonenal (4-HNE) are products of endogenous lipid peroxidation, arising as a consequence of oxidative stress...
  77. ncbi Novel electrochemical approach to enhanced toxicity of 4-oxo-2-nonenal vs. 4-hydroxy-2-nonenal (role of imine): oxidative stress and therapeutic modalities
    Peter Kovacic
    Department of Chemistry, San Diego State University, 5500 Campanile Dr, San Diego, CA 92182 1030, USA
    Med Hypotheses 67:151-6. 2006
    ..Subsequent decomposition yields various functionalized aldehydes, including 4-hydroxy-2-nonenal (HNE). OS linked to HNE is associated with various illnesses...
  78. ncbi Role of aldehyde dehydrogenase isozymes in the defense of rat lens and human lens epithelial cells against oxidative stress
    Sanjeev Choudhary
    Department of Human Biological Chemistry and Genetics, University of Texas, Galveston, 77555 0647, USA
    Invest Ophthalmol Vis Sci 46:259-67. 2005
    4-Hydroxynonenal (HNE), a metastable lipid peroxidation product, is highly toxic to various cell types if not detoxified...
  79. doi Mass spectrometric characterization of modifications to angiotensin II by lipid peroxidation products, 4-oxo-2(E)-nonenal and 4-hydroxy-2(E)-nonenal
    Seon Hwa Lee
    Department of Bioanalytical Chemistry, Graduate School of Pharmaceutical Sciences, Tohoku University, Aoba ku, Sendai, Japan
    Chem Res Toxicol 23:1771-85. 2010
    ..reactions of Ang II with lipid peroxidation-derived aldehydes, 4-oxo-2(E)-nonenal (ONE) or 4-hydroxy-2(E)-nonenal (HNE), we have identified the major modifications to the N-terminus, Asp(1), Arg(2), and His(6) of Ang II by liquid ..
  80. ncbi Degradation of glyceraldehyde-3-phosphate dehydrogenase triggered by 4-hydroxy-2-nonenal and 4-hydroxy-2-hexenal
    Yukihiro Tsuchiya
    Department of Hygienic Chemistry, Showa Pharmaceutical University, Machida, Tokyo, Japan
    Arch Biochem Biophys 438:217-22. 2005
    Lipid peroxidation products such as 4-hydroxy-2-nonenal (HNE) may be responsible for various pathophysiological events under oxidative stress, since they injure cellular components such as proteins and DNA...
  81. doi Alterations of oxidative stress markers and apoptosis markers in the striatum after transient focal cerebral ischemia in rats
    S Matsuda
    Department of Neurobiology and Therapeutics, Graduate School and Faculty of Pharmaceutical Sciences, The University of Tokushima, 1 78, Sho machi, Tokushima, 770 8505, Japan
    J Neural Transm (Vienna) 116:395-404. 2009
    ..alterations of neuronal nuclei (NeuN), Cu/Zn-SOD (superoxide dismutase), Mn-SOD, 4-hydroxy-2-nonenal (HNE), and single strand DNA (ssDNA) in the striatum from 3 h up to 15 days after transient focal cerebral ischemia in ..
  82. ncbi Catalytic function of Drosophila melanogaster glutathione S-transferase DmGSTS1-1 (GST-2) in conjugation of lipid peroxidation end products
    S P Singh
    Department of Medicine, University of Arkansas for Medical Sciences, Little Rock, AR, USA
    Eur J Biochem 268:2912-23. 2001
    ..CDNB), but has relatively high glutathione-conjugating activity for 4-hydroxynonenal (4-HNE), an electrophilic aldehyde derived from lipid peroxidation...
  83. ncbi Different onsets of oxidative damage to DNA and lipids in bone marrow and liver in rats given total body irradiation
    K Umegaki
    Department of Food Science Research for Health, The National Institute of Health and Nutrition, Tokyo, Japan
    Free Radic Biol Med 31:1066-74. 2001
    ..and lipids was evaluated by measuring increases of 8-hydroxydeoxyguanosine (8OHdG) in DNA and 4-hydroxy-2-nonenal (HNE), respectively...
  84. ncbi Parameters that affect in vitro splicing of bovine papillomavirus type 1 late pre-mRNAs
    Z M Zheng
    Basic Research Laboratory, Division of Basic Sciences, National Cancer Institute National Institutes of Health, Bethesda, MD 20892, USA
    J Virol Methods 85:203-14. 2000
    ..The optimal concentration of HeLa nuclear extract (HNE) in a standard RNA splicing reaction depends on the individual substrate pre-mRNA...
  85. ncbi Collagen type I synthesized by pancreatic periacinar stellate cells (PSC) co-localizes with lipid peroxidation-derived aldehydes in chronic alcoholic pancreatitis
    A Casini
    Alcohol Research Center and Gastroenterology Unit, Department of Clinical Pathophysiology, University of Florence, Florence, Italy
    J Pathol 192:81-9. 2000
    ..It has been recently suggested that lipid peroxidation-derived aldehydes such as 4-hydroxynonenal (HNE) are involved in tissue damage and fibrosis in other organs...
  86. ncbi Regulation of GSH in alphaA-expressing human lens epithelial cell lines and in alphaA knockout mouse lenses
    R Kannan
    Division of Gastrointestinal and Liver Diseases, University of Southern California Keck School of Medicine, Los Angeles, CA 90033, USA
    Invest Ophthalmol Vis Sci 42:409-16. 2001
    To study the mechanism of regulation of GSH in HLE-B3 cells expressing alphaA-crystallin (alphaA) and in alphaA knockout mouse lenses.
  87. ncbi Protein adducts of malondialdehyde and 4-hydroxynonenal in livers of iron loaded rats: quantitation and localization
    M Firoze Khan
    Department of Pathology, University of Texas Medical Branch, Galveston, TX 77555 0609, USA
    Toxicology 173:193-201. 2002
    ..catalyze peroxidation of lipids to produce reactive aldehydes such as malondialdehyde (MDA) and 4-hydroxynonenal (HNE). Covalent binding of such lipid aldehydes with proteins may cause impairment in cellular function and integrity...
  88. ncbi HNE Michael adducts to histidine and histidine-containing peptides as biomarkers of lipid-derived carbonyl stress in urines: LC-MS/MS profiling in Zucker obese rats
    Marica Orioli
    Istituto di Chimica Farmaceutica e Tossicologica Pietro Pratesi, Faculty of Pharmacy, University of Milan, Via Mangiagalli 25, I 20133 Milan, Italy
    Anal Chem 79:9174-84. 2007
    ..in free form only, have been identified, two of them originating from the conjugation of 4-hydroxy-trans-2-nonenal (HNE) to His, followed by reduction/oxidation of the aldehyde: His-1,4-dihydroxynonane (His-DHN), His-4-hydroxynonanoic ..
  89. pmc Estimating the disease burden of pandemic (H1N1) 2009 virus infection in Hunter New England, Northern New South Wales, Australia, 2009
    Fatimah S Dawood
    Influenza Division, Centers for Disease Control and Prevention, Atlanta, Georgia, United States of America
    PLoS ONE 5:e9880. 2010
    On May 26, 2009, the first confirmed case of Pandemic (H1N1) 2009 virus (pH1N1) infection in Hunter New England (HNE), New South Wales (NSW), Australia (population 866,000) was identified...
  90. ncbi Apoptosis of PC12 cells by 4-hydroxy-2-nonenal is mediated through selective activation of the c-Jun N-terminal protein kinase pathway
    B J Song
    Laboratory of Membrane Biochemistry and Biophysics, National Institute on Alcohol Abuse and Alcoholism, 12420 Parklawn Drive, 20852, Rockville, MD, USA
    Chem Biol Interact 130:943-54. 2001
    Cytotoxic lipid peroxides such as 4-hydroxy-2-nonenal (HNE) are produced when cells are exposed to toxic chemicals. However, the mechanism by which HNE induces cell death has been poorly understood...
  91. ncbi Earthworm fibrinolytic enzyme: anti-tumor activity on human hepatoma cells in vitro and in vivo
    Hong Chen
    Department of Gastroenterology, Affiliated Zhongda Hospital, Southeast University, Nanjing 210009, China
    Chin Med J (Engl) 120:898-904. 2007
    ..In this study, the anti-tumor activity of EFE, isolated from Eisenia foetida, on human hepatoma cells was evaluated in vitro and in vivo. The potential mechanisms involved were also studied...
  92. doi Enhanced expression of haemoglobin scavenger receptor in accumulated macrophages of culprit lesions in acute coronary syndromes
    Kei Yunoki
    Department of Cardiology, Osaka City General Hospital, Osaka, Japan
    Eur Heart J 30:1844-52. 2009
    ..We immunohistochemically examined the relationship between intraplaque haemorrhage, 4-HNE (4-hydroxy-2-nonenal), an index of lipid peroxidation, and the Hb scavenger receptor (CD163), using coronary ..
  93. doi Demonstration of ethanol-induced protein adducts in oral leukoplakia (pre-cancer) and cancer
    Saman Warnakulasuriya
    Department of Oral Medicine, King s College London Dental Institute at Guy s, King s and St Thomas Hospitals, WHO Collaborating Centre for Oral Cancer and Precancer, London, UK
    J Oral Pathol Med 37:157-65. 2008
    ..Excessive alcohol consumption is a common cause for upper gastrointestinal tract cancers. However, the primary mechanisms of alcohol-induced carcinogenesis have remained poorly defined...
  94. doi Influence of cytotoxic doses of 4-hydroxynonenal on selected neurotransmitter receptors in PC-12 cells
    M A Siddiqui
    In Vitro Toxicology Laboratory, Industrial Toxicology Research Centre, P O Box 80, MG Marg, Lucknow 226001, UP, India
    Toxicol In Vitro 22:1681-8. 2008
    Effect of 4-hydroxynonenal (HNE), a long-chain alpha, beta unsaturated aldehyde product, generated by the oxidation of omega-6 polyunsaturated fatty acids on the sensitivity of selected neurotransmitter receptors was studied in PC-12 ..
  95. doi Role of human aldo-keto-reductase AKR1B10 in the protection against toxic aldehydes
    Hans Jörg Martin
    Institute of Toxicology and Pharmacology for Natural Scientists, University Medical School Schleswig Holstein, Campus Kiel, Kiel, Germany
    Chem Biol Interact 178:145-50. 2009
    ..Among the best characterized intermediates of oxidative stress are the unsaturated aldehydes 4-hydroxynon-2-enal (4-HNE) and its oxidized counterpart 4-oxonon-2-enal (4-ONE)...
  96. doi Western blot analysis for 4-hydroxy-2-nonenal (HNE)-modified proteins in paraquat-treated mice
    Emiko Kurisaki
    Department of Legal Medicine, Fukushima Medical University School of Medicine, Hikarigaoka 1, Fukushima 960 1125, Japan
    Leg Med (Tokyo) 11:S431-3. 2009
    ..In this study, we investigated changes in 4-hydroxy-2-nonenal (HNE)-modified proteins as markers of lipid peroxidation in PQ-treated mice...
  97. pmc Teratogen-induced oxidative stress targets glyceraldehyde-3-phosphate dehydrogenase in the organogenesis stage mouse embryo
    Ava E Schlisser
    Department of Pharmacology and Therapeutics, McGill University, Montreal, Quebec, Canada H3G 1Y6
    Toxicol Sci 118:686-95. 2010
    ..with polyunsaturated bilipid membranes to form α,β-unsaturated reactive aldehydes; 4-hydroxy-2-nonenal (4-HNE), one of the most cytotoxic of these aldehydes, covalently adducts with proteins, lipids, and nucleic acids...
  98. ncbi Differential expression and comparative subcellular localization of estrogen receptor beta isoforms in virally transformed and normal cultured human lens epithelial cells
    Patrick R Cammarata
    Department of Cell Biology and Genetics, University of North Texas Health Science Center, Fort Worth, TX 76107, USA
    Exp Eye Res 81:165-75. 2005
    ..In this study, we determined whether virally transformed cell cultures of human lens epithelial cells (HLE-B3) express both full length (or wt) and variant isoforms of ERbeta in comparison to normal secondary cultures of ..
  99. ncbi Characterization of 4-hydroxy-2-nonenal metabolism in stellate cell lines derived from normal and cirrhotic rat liver
    J F Reichard
    School of Pharmacy, University of Colorado Health Sciences Center, Denver, CO 80262, USA
    Biochim Biophys Acta 1487:222-32. 2000
    During oxidative stress, reactive aldehydes, including trans-4-hydroxy-2-nonenal (4-HNE), are generated by peroxidation of membrane lipids and purportedly stimulate hepatic stellate cells to produce excessive extracellular matrix, ..
  100. pmc Effects of fibril- or fixed-collagen on matrix metalloproteinase-1 and tissue inhibitor of matrix metalloproteinase-1 production in the human hepatocyte cell line HLE
    Makoto Nakamuta
    Department of Medicine and Bioregulatory Science, Graduate School of Medical Sciences, Kyushu University, 3 1 1 Maidashi, Higashi ku, Fukuoka 812 8582, Japan
    World J Gastroenterol 11:2264-8. 2005
    ..The mechanisms involved have been investigated in hepatic stellate cells (HSC), but not in hepatocytes. We investigated the effects of fibril- and fixed-collagen on MMP-1 and TIMP-1 production in hepatocytes, using the HLE cell line.
  101. ncbi Glutathione homeostasis in human hepatic cells: overexpression of gamma-glutamylcysteine synthetase gene in cell lines resistant to buthionine sulfoximine, an inhibitor of glutathione synthesis
    T Tanaka
    Department of Biochemistry, Kyushu University School of Medicine, Fukuoka, Japan
    Biochem Biophys Res Commun 246:398-403. 1998
    ..understand how cellular levels of GSH are balanced in response to environmental stress, we cloned two cell lines, HLE/BSO1-1 and HLE/BSO1-2, from human hepatic HLE/WT cells resistant to buthionine sulfoximine (BSO), an irreversible ..

Research Grants63

  1. ARUNI BHATNAGAR BHATNAGAR; Fiscal Year: 2015
    ..To identify changes in aldehyde metabolism, metabolites of 4-hydroxy-trans-2-nonenal (HNE) generated in hypertrophic hearts perfused with radiolabeled HNE will be identified and quantified along with HNE ..
  2. Lipid peroxidation product induced DNA damage in the p53 gene and liver cancer
    ERIC MOON SHONG M TANG; Fiscal Year: 2010
    ..of aldehydes, such as acrolein (Acr), crotonaldehyde, malondialdehyde (MDA), and trans-4-hydroxy- 2-nonenal (4-HNE), that are able to interact with DNA;these adducts induce mainly G to T transversion...
  3. Role and Molecular Mechanisms of Corneal Aldehyde Dehydrogenase
    Vasilis Vasiliou; Fiscal Year: 2013
    ..that leads to membrane lipid peroxidation and accumulation of toxic aldehydes, such as 4-hydroxy-2- nonenal (4-HNE)...
  4. Cardioprotective Mechanisms of Glutathione S-transferase P
    DANIEL JOSEPH CONKLIN; Fiscal Year: 2012
    ..I/R in situ (Aim 1), metabolism of lipid peroxidation- derived aldehydes (acrolein and 4-hydroxy-trans-2-nonenal;HNE), which are the preferred endogenous substrates of this enzyme (Aim 2), and aldehyde detoxication (Aim 3)...
  5. Detoxification of Xenobiotics in Ocular Tissue
    YOGESH CHANDRA AWASTHI; Fiscal Year: 2010
    Toxic lipid aldehydes such as 4-hydroxynonenal (HNE), formed during lipid peroxidation (LPO), are involved in the etiology of cataractogenesis and age related pathophysiology of ocular tissues...
  6. RICHARD MICHAEL LOPACHIN; Fiscal Year: 2015
    ..g., acrolein, 4-hydroxy-2-nonenal (HNE) and methyl acrylate (MA)...
  7. Zhanxiang Zhou; Fiscal Year: 2014
    ..alcohol metabolism generates acetaldehyde, lipid peroxidation produces lipid aldehydes such as 4-hydroxynonenal (4-HNE) and malondialdehyde (MDA)...
  8. Aldose Reductase: A Regulator of Inflammatory Signals
    KOTA VENKATA RAMANA; Fiscal Year: 2011
    ..4-hydroxy trans-2-nonenal (HNE), one of the most toxic and abundant LDA generated during lipid peroxidation and GS-HNE are efficiently reduced by ..
  9. LIPID PEROXIDATION\CELL SIGNALING-CC14-INDUCED FIBROSIS
    DENNIS PETERSEN; Fiscal Year: 2004
    ..project is to establish the mechanistic role(s) of aldehydic products of lipid peroxidation (4-hydroxynonenal [4-HNE] and malondialdehyde [MDA]) in chemical-induced liver fibrosis...
  10. Modification of PDI by 4-HNE and 4-ONE and its Role in Ethanol-Induced ER Stress
    James J Galligan; Fiscal Year: 2011
    ..Published data has shown modification of the active-site of PDI by the reactive aldehyde 4-hydroxynonenal (4-HNE)...
  11. Role of Aldose Reductase in Diabetic Complications
    Satish K Srivastava; Fiscal Year: 2013
    ..This will help in developing structure-based AR inhibitors which will prevent the binding of GS-HNE without affecting the binding and reduction of toxic lipid aldehydes such as HNE...
  12. Atherogenic mechanism of lipid peroxidation-derived aldehydes
    Sanjay Srivastava; Fiscal Year: 2013
    ..Aldehydes such as 4-hydroxynonenal (HNE) and 1-palmitoyl-2-(5-oxovaleroyl)-3- glycero- phosphatidyl choline (POVPC) are the major bioactive products ..
  13. Localized Metal Modulation as a First Line Treatment of Burn Injuries
    JERRY GIN; Fiscal Year: 2012
    ..increased lipid peroxidation and iron dependent formation of cytotoxic lipid aldehydes, such as 4- hydroxynonenal (HNE) that can induce Ca/Fe-dependent toxic events...
  14. Modifications of L-FABP by Reactive Aldehydes in a Model of Chronic ALD
    REBECCA LEANNE SMATHERS; Fiscal Year: 2011
    ..chronic ethanol consumption leads to the covalent modification of liver fatty acid binding protein (L-FABP) by 4-HNE and 4-ONE, which ultimately leads to the disruption of lipid metabolism and pathogenesis of steatosis in ALD...
  15. Endothelial Metabolism of Atherogenic Aldehydes
    Sanjay Srivastava; Fiscal Year: 2005
    ..aldehydes we will use 1-palmitoyl-2-(-5-oxovaleryl)-3-glycero phosphocholine (POVPC) and 4-hydroxy-trans-2-nonenal (HNE) as model aldehydes...
  16. GSTs: Oxidative Stress in Early Atherosclerosis
    Paul Boor; Fiscal Year: 2006
    ..response to electrophilic injury caused by alpha, beta-unsaturated aldehydes such as acrolein, 4-hydroxynonenal (4-HNE) and 4-hydroxyhexenal (4-HHE), which are toxic end products of lipid peroxidation implicated in the pathogenesis of ..
  17. Lipid electrophile adduction of Pin1 and effects on substrate binding &activity
    Christopher D Aluise; Fiscal Year: 2013
    ..Examples of lipid electrophiles that have been extensively studied are 4-hydroxynonenal (4-HNE), 4- oxononenal (4-ONE), acrolein, and malondialdehyde...
  18. Proteomic profiling of NASH: disease mechanisms and novel treatment
    DENNIS PETERSEN; Fiscal Year: 2011
    ..peroxidation giving rise to bioactive aldehydic products of lipid peroxidation including 4-hydroxy-2-nonenal (4-HNE) and 4-oxononenal (4-ONE)...
  19. Protection of Oxidant Toxicity by GSTs
    YOGESH CHANDRA AWASTHI; Fiscal Year: 2010
    4-Hydroxynonenal (4-HNE), a stable end-product of lipid peroxidation (LPO) can cause toxicity through apoptosis and necrosis...
  20. Reactive Aldehyde DNA Damage in Mitochondrial Mutagenesis and Disease Pathophysio
    SUSAN CLINE; Fiscal Year: 2009
    ..and nucleic acids to release a host of reactive aldehydes, including malondialdehyde (MDA), 4-hydroxynonenal (4-HNE), and nucleic acid base propenals [1,12, 25]...
  21. LIPID ALDEHYDES AND ETHANOL INDUCED LIVER DAMAGE
    DENNIS PETERSEN; Fiscal Year: 2005
    ..project is to establish the mechanistic role(s) of aldehydic products of lipid peroxidation (4-hydroxynonenal [4-HNE] and malondialdehyde [MDA] in alcoholic liver disease (ALD)...
  22. Randy Strong; Fiscal Year: 2016
    ..that impaired aldehyde detoxification leads to elevated "aldehyde load" including increased levels of DOPAL and 4-HNE. These aldehydes or their metabolites can form adducts with a-synuclein, leading to formation of toxic fibrils and ..
  23. Hydroxynonenal induces glutathione synthesis through JNK
    Henry Jay Forman; Fiscal Year: 2010
    ..as it is critical in removal of hydroperoxides and toxic lipid peroxidation products, such as 4-hydroxynonenal (HNE)...
  24. Role of human DNA polymerase kappa in replicative bypass of DNA lesions
    Satya Prakash; Fiscal Year: 2013
    ..N2-(3-hydroxyl propyl-2'-deoxygunaosine [(r)-3HOPdG] and the bulky trans-4-hydroxy-2-non-enal- deoxyguanosine (HNE-dG) adducts that result from the reaction of N2-dG with aldehydes or enals generated from free radical attack on ..
  25. IDENTIFICATION OF TUMOR PROMOTION SUSCEPTIBILITY GENES
    John DiGiovanni; Fiscal Year: 2013
    ..Gsta4 is a glutathione-S-transferase (GST) and a major substrate for Gsta4 is 4-hydroxy-2(E)-nonenal (4-HNE), a product of lipid peroxidation...
  26. DENNIS PETERSEN; Fiscal Year: 2016
    ..and functionally characterizing proteins modified by the lipid peroxidative products 4-hydroxynonenai (4-HNE)...
  27. Detoxification Role of Retinol Dehydrogenases RDH11 and RDH12
    Anne Kasus Jacobi; Fiscal Year: 2009
    ..4-hydroxy-trans-2-nonenal (4-HNE) is one of the major aldehydic products and is an inducer and mediator of oxidative stress...
  28. BIOCHEMICAL MECHANISM OF OXIDATIVE CATARACTOGENESIS
    Naseem Ansari; Fiscal Year: 2006
    ..Under oxidative stress, these tissues generate lipid-derived aldehydes (LDAs) such as 4-hydroxynonenal (HNE) which are known to propagate the ROS-induced toxicity...
  29. Ascorbylation of Oxidized Lipids and Atherosclerosis
    Jan Frederik Stevens; Fiscal Year: 2010
    ..central hypothesis to be evaluated is that vitamin C eliminates harmful LPO products, such as 4-hydroxy-2-nonenal (HNE), through ascorbylation (conjugate formation), resulting in an overall anti-atherogenic effect on the vascular wall...
  30. NEURAL REGULATION OF PANCREATIC FUNCTION
    KIMBERLY SAUNDERS KIRKWOOD; Fiscal Year: 2012
    ..We have recently discovered that 4-hydroxynonenal (HNE), an aldehyde generated by the action of reactive oxygen species (ROS) on membrane lipids, is an endogenous agonist ..
  31. Sulfated Polysaccharide Derivatives for the Treatment of Rosacea
    NARAYANAM RAO; Fiscal Year: 2009
    ..activities at nanogram/ml concentrations, including inhibition of the cationic PMN protease human leukocyte elastase (HLE) and inhibition of the PMN adhesion receptor P-selectin...
  32. Sulfated Polysaccharide Derivatives for Treatment of Macular Degeneration
    Jianxing Zhang; Fiscal Year: 2009
    ..at nanogram/ml concentrations, including inhibition of complement activation, P-selectin and human leukocyte elastase (HLE)...
  33. ALPHA-1-PROTEINASE INHIBITOR DEFICIENCY AND EMPHYSEMA
    Savio Woo; Fiscal Year: 1990
    ..is to defuse into the lung and protect this important organ from destruction by excessive polymorphonuclear leukocyte elastase. Genetic deficiency of Alpha1-antitrypsin predisposes affected individuals to development of chronic ..
  34. Glutathione S-Transferases and Oxidative Stress
    William M Atkins; Fiscal Year: 2011
    ..GSH) to several lipid or prostaglandin peroxidation products of oxidative stress, such as 4-hydroxynonenal (HNE), 13-Oxo, or isoprostanoids (Isops)...
  35. DNA Repair of Endogenous Lesions in Carcinogenesis
    Rabindra Roy; Fiscal Year: 2010
    ..that observed in tissues obtained from Aim 1;(4) study capacity to repair cyclic propano adducts (Acr-, Cro-, and HNE-dG) in LEG and LEA rat livers and lungs as control...
  36. Targeted investigation of tissue specific oxidative stress in the etiology of ALS
    Holly Van Remmen; Fiscal Year: 2013
    ..will also measure oxidative damage to lipid (F2-isoprostanes, neuroprostanes), DNA (oxo8dG) and protein (carbonyls, HNE adducts) in spinal cord and gastrocnemius muscle at pre-symptomatic, onset and end stages of the disease to ..
  37. Lipid-Peroxidation Induced Cyclic Adducts in Hepatocarcinogenesis
    Fung Lung Chung; Fiscal Year: 2013
    ..by applicant): Cyclic 1,N2-propanodeoxyguanosine adducts of acrolein (Acr-dG) and trans-4-hydroxy-2-nonenal (HNE-dG) derived from lipid peroxidation have been detected as endogenous DNA lesions in rodent and human tissues...
  38. REACTIVE ALDEHYDE BIOMARKERS OF LIPID PEROXIDATION
    Sidney Nelson; Fiscal Year: 1992
    ..hydrazine derivatives of two major aldehydes of lipid peroxidation, malondialdehyde (MDA) and 4-hydroxynonenal (4-HNE), 2) to synthesize, characterize, and develop assays (HPLC with fluorometric detection) for amino acid adducts of ..
  39. RESEARCH CENTER FOR PHARMACOLOGY AND DRUG TOXICOLOGY
    L Jackson Roberts; Fiscal Year: 2010
    ..Project 4 will focus on mechanisms by which this compound is formed in vivo and characterize novel HNE adducts...
  40. The role of MRP1 in Protection of Cardiac Injury
    Mary Vore; Fiscal Year: 2012
    ..and its conjugates, including the GSH conjugate of the cytotoxic product of oxidative stress, 4-hydroxynonenal (HNE;GS-HNE)...
  41. Isotropic Reinforcement to Minimize Nigrostriatal Degeneration
    AMY BEATRICE MANNING-BOG; Fiscal Year: 2013
    ..Toxic reactive oxygen species (ROS) and downstream reactive carbonyl products (RCP) such as 4-hydroxynonenal (4-HNE), are generated in mitochondria...
  42. HNE damage of adenine nucleotide translocase in ethanol-mediated neuron apoptosis
    George I Henderson; Fiscal Year: 2010
    ..apoptosis activation, is perturbation of adenine nucleotide translocase (ANT) function by 4- hydroxynonenal (HNE) adduct formation...
  43. Quantitative proteomic profiling of reactive cysteines in oxidative stress
    Chu Wang; Fiscal Year: 2012
    ..or ozone, peroxidation of membrane lipids yields several reactive end products, among which 4-hydroxy-2-nonenal (HNE) is a major species...
  44. Causative role of lipid peroxidation products in obesity and aging
    Sharda P Singh; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): The lipid peroxidation product 4-hydroxynonenal (4-HNE) is a signaling molecule affecting several fundamental biological processes, including aging...
  45. Superoxide Generation from eNOS: The Role of Pterins
    JEANNETTE M VASQUEZ VIVAR; Fiscal Year: 2010
    ..in endothelial cells increases superoxide production by calcium-dependent mechanisms;(iii) 4-hydroxy-2-nonenal (HNE) is a potent inhibitor of BH4 synthesis, depletes BH4 and NO, and increases superoxide production in endothelial ..
  46. Edward D Hall; Fiscal Year: 2016
    ..shown that treatment with the potent LP inhibitor U-83836E, with the LP-derived lipid aldehyde 4-hydroxynonenal (4-HNE) scavenger phenelzine or with the mitochondrial permeability transition inhibitor cyclosporine (CsA) can partially ..
  47. Inhibition of Lipid Peroxidation in SCI
    Edward D Hall; Fiscal Year: 2012
    ..strategy that combines a LP inhibitor U-83836E with the LP-derived lipid aldehyde 4-hydroxynonenal (4-HNE) scavenger phenelzine to achieve a greater degree of neuroprotection...
  48. Molecular Mechanism of Severe Congenital Neutropenia
    H Grimes; Fiscal Year: 2009
    ..The majority of SCN patients express mutant forms of neutrophil elastase (a serine protease encoded by ELA2)...
  49. LOW DENSITY LIPOPROTEIN AND INFLAMMATION
    Robert Terkeltaub; Fiscal Year: 1993
    ..in solid-phase RIAs for three epitopes present in oxidized LDL: (malondialdehyde(MDA)-lysine, 4-hydroxynonenal(HNE)-lysine, and a unique apo B epitope). Certain new apo B epitopes in oxidized LDL are highly immunogenic, e.g...
  50. JUSTIN RODNEY SAVAGE; Fiscal Year: 2015
    ..are systemically and topically safe anti-inflammatory agents that block P- and L-selectin, inhibit human neutrophil elastase (HLE), block complement receptor-3 (CR3, also known as Mac-1), and block interaction of RAGE with its known ..
  51. OXIDATION INDUCED MEMBRANE DAMAGE IN CATARACTOGENESIS
    KAILASH BHUYAN; Fiscal Year: 1999
    ..lipid-derived oxidant species such as conjugated dienes, LOOH, malondialdehyde (MDA) and 4-hydroxynonenal (4-HNE), by UV spectrophotometry and HPLC in normal lenses of human donor eyes as a function of age...
  52. LIVER PEROXIDATION PRODUCTS AND LIVER FIBROSIS
    Frank Anania; Fiscal Year: 2003
    ..In recent investigations, products of oxidative stress, including malondialdehyde (MDA) and 4-hydroxy-2- nonenal (HNE) can augment type I Collagen gene expression in activated stellate cells, however, the potential signal ..
  53. 4-HNE and MDA adduction of SREBP-1 and PPAR-alpha in ALD
    Benjamin Stewart; Fiscal Year: 2007
    ..and peroxisome proliferator-activated receptor-alpha (PPARalpha), The lipid aldehydes 4-hydroxy-2-nonenal (4-HNE) and malondialdehyde (MDA) are generated in response to chronic ethanol consumption, and are capable of covalently ..
  54. MECHANISM OF ANTICARCINOGENIC EFFECT OF ANTIOXIDANTS
    YOGESH AWASTHI; Fiscal Year: 2000
    ..g. the genotoxic 4-hydroxynonenal (4- HNE), that are 50-100 fold higher than those of any other known GSTs, and also have GSH-peroxidase activity towards ..
  55. Regulation of PTEN in chronic alcohol mediated liver disease
    Colin Shearn; Fiscal Year: 2012
    ..Our working hypothesis states the reactive aldehyde 4-HNE, produced in the liver of chronic ethanol treated mice covalently adducts PTEN thereby reducing enzymatic activity ..
  56. RECOMBINANT SERPINS AND NEUTROPHILS IN ACUTE LUNG INJURY
    Michael Plotnick; Fiscal Year: 2000
    ..Neutrophil serine proteases, especially elastase (HNE), have been implicated as major mediators or acute lung injury...
  57. SERINE PROTEASE INHIBITORS & MECHANISMS OF INFLAMATION
    Harvey Rubin; Fiscal Year: 2004
    ..In particular, we are interested the regulation of human neutrophil elastase (HNE) because of its well documented role in inflammation...
  58. Alpha-1 AD Carriers and Lung Cancer Risk
    Ping Yang; Fiscal Year: 2009
    ..and lung cancer risk, it is critical to investigate the role of the ELA2 gene that encodes neutrophil elastase, the natural counterpart of alpha1-antitrypsin...
  59. CHEMISTRY AND BIOLOGY OF CARCINOGEN-DNA ADDUCTS
    Michael P Stone; Fiscal Year: 2010
    ..endogenously-produced bis-electrophiles, such as vinyl chloride, acrolein, crotonaldehyde, and 4- hydroxynonenal (4-HNE), induce genotoxic response, ultimately impacting human health...
  60. ENALS IN TUMORIGENESIS
    Fung Lung Chung; Fiscal Year: 2010
    ..deoxyguanosine adducts, the major sites of modification in DNA by enals: acrolein (Acr-dG), t-4-hydroxy-2-nonenal (HNE-dG), and malondialdehyde (MdG)...
  61. Human Cathepsin G: Expression, C-Terminal Processing and Dual Specificity
    David Johnson; Fiscal Year: 2007
    unreadable] DESCRIPTION (provided by applicant): Human Cathepsin G (CatG) and human neutrophil elastase (HNE) play significant innate immunity roles via neutrophil killing of bacteria...
  62. ALCOHOL METABOLISM--ROLE OF P450 OXYGENASES
    Minor Coon; Fiscal Year: 2000
    ..study the inactivation of ethanol-inducible P450 and other microsomal P450 isozymes by trans-4-hydroxy-2-nonenal (HNE) and related products of membrane lipid peroxidation...
  63. Novel Method to Detect Pathogens for the Diagnosis of Chronic Wound Infection
    JAMES COMOLLI; Fiscal Year: 2007
    ..This work also revealed some cross-reactivity of the target peptide with the human neutrophil elastase (HNE), a host inflammatory protease present at high levels in chronic wounds...