elafin

Summary

Gene Symbol: elafin
Description: peptidase inhibitor 3
Alias: ESI, SKALP, WAP3, WFDC14, cementoin, elafin, PI-3, WAP four-disulfide core domain 14, WAP four-disulfide core domain protein 14, elastase-specific inhibitor, peptidase inhibitor 3, skin-derived, pre-elafin, protease inhibitor 3, skin-derived (SKALP), protease inhibitor WAP3, skin-derived antileukoproteinase, trappin-2
Species: human
Products:     elafin

Top Publications

  1. Li Q, Zhou X, Nie X, Yang J. The role of recombinant human elafin in the resistance of A549 cells against Pseudomonas aeruginosa biofilm. Respiration. 2010;79:68-75 pubmed publisher
    ..b>Elafin, a natural antibiotic, has antimicrobial properties against Gram-negative bacteria...
  2. Tsunemi M, Matsuura Y, Sakakibara S, Katsube Y. Crystal structure of an elastase-specific inhibitor elafin complexed with porcine pancreatic elastase determined at 1.9 A resolution. Biochemistry. 1996;35:11570-6 pubmed
    The crystal structure of a stoichiometric complex between an elastase-specific inhibitor elafin and porcine pancreatic elastase (PPE) has been determined and refined to a crystallographic R-factor of 19.7% at 1.9 A resolution...
  3. King A, Critchley H, Sallenave J, Kelly R. Elafin in human endometrium: an antiprotease and antimicrobial molecule expressed during menstruation. J Clin Endocrinol Metab. 2003;88:4426-31 pubmed
    b>Elafin is an antiproteinase and antimicrobial molecule that is expressed at epithelial sites (for example, cervix). This study details the expression and regulation of elafin in the human endometrium...
  4. McMichael J, Roghanian A, Jiang L, Ramage R, Sallenave J. The antimicrobial antiproteinase elafin binds to lipopolysaccharide and modulates macrophage responses. Am J Respir Cell Mol Biol. 2005;32:443-52 pubmed
    ..We demonstrate here that the antimicrobial/anti-neutrophil elastase full-length elafin (FL-EL) is able to bind both smooth and rough forms of LPS...
  5. Sallenave J, Ryle A. Purification and characterization of elastase-specific inhibitor. Sequence homology with mucus proteinase inhibitor. Biol Chem Hoppe Seyler. 1991;372:13-21 pubmed
    Elastase-specific inhibitor (ESI) was purified from sputum of patients with chronic bronchitis and compared with mucus proteinase inhibitor (MPI, BrI) isolated, without the use of affinity chromatography on an enzyme, from non-purulent ..
  6. Ghosh M, Shen Z, Fahey J, Cu Uvin S, Mayer K, Wira C. Trappin-2/Elafin: a novel innate anti-human immunodeficiency virus-1 molecule of the human female reproductive tract. Immunology. 2010;129:207-19 pubmed publisher
    Trappin-2/Elafin is a serine protease inhibitor that plays a major role as an anti-inflammatory mediator at mucosal surfaces...
  7. Molhuizen H, Alkemade H, Zeeuwen P, de Jongh G, Wieringa B, Schalkwijk J. SKALP/elafin: an elastase inhibitor from cultured human keratinocytes. Purification, cDNA sequence, and evidence for transglutaminase cross-linking. J Biol Chem. 1993;268:12028-32 pubmed
    SKALP/Elafin is a proteinase inhibitor found in psoriatic epidermis as a short polypeptide of 6 kDa. Here we present evidence that this protein is synthesized as a larger precursor molecule with distinct biological features...
  8. Zhang M, Zou Z, Maass N, Sager R. Differential expression of elafin in human normal mammary epithelial cells and carcinomas is regulated at the transcriptional level. Cancer Res. 1995;55:2537-41 pubmed
    b>Elafin is an elastase inhibitor with a unique structure, not related to the serpin family, which includes the neutrophil elastase inhibitor...
  9. Wang Z, Beach D, Su L, Zhai R, Christiani D. A genome-wide expression analysis in blood identifies pre-elafin as a biomarker in ARDS. Am J Respir Cell Mol Biol. 2008;38:724-32 pubmed publisher
    ..Among 126 differentially expressed genes, peptidase inhibitor 3 (PI3, encoding elafin, a potent neutrophil elastase inhibitor) had the largest fold-change (-3-fold changes, acute stage/recovery stage) ..
  10. Clauss A, Lilja H, Lundwall A. A locus on human chromosome 20 contains several genes expressing protease inhibitor domains with homology to whey acidic protein. Biochem J. 2002;368:233-42 pubmed
    ..1. Among them are genes of the known or postulated protease inhibitors elafin, secretory leucocyte protease inhibitor, human epididymis gene product 4, eppin, and huWAP2...

Detail Information

Publications98

  1. Li Q, Zhou X, Nie X, Yang J. The role of recombinant human elafin in the resistance of A549 cells against Pseudomonas aeruginosa biofilm. Respiration. 2010;79:68-75 pubmed publisher
    ..b>Elafin, a natural antibiotic, has antimicrobial properties against Gram-negative bacteria...
  2. Tsunemi M, Matsuura Y, Sakakibara S, Katsube Y. Crystal structure of an elastase-specific inhibitor elafin complexed with porcine pancreatic elastase determined at 1.9 A resolution. Biochemistry. 1996;35:11570-6 pubmed
    The crystal structure of a stoichiometric complex between an elastase-specific inhibitor elafin and porcine pancreatic elastase (PPE) has been determined and refined to a crystallographic R-factor of 19.7% at 1.9 A resolution...
  3. King A, Critchley H, Sallenave J, Kelly R. Elafin in human endometrium: an antiprotease and antimicrobial molecule expressed during menstruation. J Clin Endocrinol Metab. 2003;88:4426-31 pubmed
    b>Elafin is an antiproteinase and antimicrobial molecule that is expressed at epithelial sites (for example, cervix). This study details the expression and regulation of elafin in the human endometrium...
  4. McMichael J, Roghanian A, Jiang L, Ramage R, Sallenave J. The antimicrobial antiproteinase elafin binds to lipopolysaccharide and modulates macrophage responses. Am J Respir Cell Mol Biol. 2005;32:443-52 pubmed
    ..We demonstrate here that the antimicrobial/anti-neutrophil elastase full-length elafin (FL-EL) is able to bind both smooth and rough forms of LPS...
  5. Sallenave J, Ryle A. Purification and characterization of elastase-specific inhibitor. Sequence homology with mucus proteinase inhibitor. Biol Chem Hoppe Seyler. 1991;372:13-21 pubmed
    Elastase-specific inhibitor (ESI) was purified from sputum of patients with chronic bronchitis and compared with mucus proteinase inhibitor (MPI, BrI) isolated, without the use of affinity chromatography on an enzyme, from non-purulent ..
  6. Ghosh M, Shen Z, Fahey J, Cu Uvin S, Mayer K, Wira C. Trappin-2/Elafin: a novel innate anti-human immunodeficiency virus-1 molecule of the human female reproductive tract. Immunology. 2010;129:207-19 pubmed publisher
    Trappin-2/Elafin is a serine protease inhibitor that plays a major role as an anti-inflammatory mediator at mucosal surfaces...
  7. Molhuizen H, Alkemade H, Zeeuwen P, de Jongh G, Wieringa B, Schalkwijk J. SKALP/elafin: an elastase inhibitor from cultured human keratinocytes. Purification, cDNA sequence, and evidence for transglutaminase cross-linking. J Biol Chem. 1993;268:12028-32 pubmed
    SKALP/Elafin is a proteinase inhibitor found in psoriatic epidermis as a short polypeptide of 6 kDa. Here we present evidence that this protein is synthesized as a larger precursor molecule with distinct biological features...
  8. Zhang M, Zou Z, Maass N, Sager R. Differential expression of elafin in human normal mammary epithelial cells and carcinomas is regulated at the transcriptional level. Cancer Res. 1995;55:2537-41 pubmed
    b>Elafin is an elastase inhibitor with a unique structure, not related to the serpin family, which includes the neutrophil elastase inhibitor...
  9. Wang Z, Beach D, Su L, Zhai R, Christiani D. A genome-wide expression analysis in blood identifies pre-elafin as a biomarker in ARDS. Am J Respir Cell Mol Biol. 2008;38:724-32 pubmed publisher
    ..Among 126 differentially expressed genes, peptidase inhibitor 3 (PI3, encoding elafin, a potent neutrophil elastase inhibitor) had the largest fold-change (-3-fold changes, acute stage/recovery stage) ..
  10. Clauss A, Lilja H, Lundwall A. A locus on human chromosome 20 contains several genes expressing protease inhibitor domains with homology to whey acidic protein. Biochem J. 2002;368:233-42 pubmed
    ..1. Among them are genes of the known or postulated protease inhibitors elafin, secretory leucocyte protease inhibitor, human epididymis gene product 4, eppin, and huWAP2...
  11. Moreau T, Baranger K, Dadé S, Dallet Choisy S, Guyot N, Zani M. Multifaceted roles of human elafin and secretory leukocyte proteinase inhibitor (SLPI), two serine protease inhibitors of the chelonianin family. Biochimie. 2008;90:284-95 pubmed
    b>Elafin and SLPI are low-molecular weight proteins that were first identified as protease inhibitors in mucous fluids including lung secretions, where they help control excessive proteolysis due to neutrophil serine proteases (elastase, ..
  12. Doucet A, Bouchard D, Janelle M, Bellemare A, Gagné S, Tremblay G, et al. Characterization of human pre-elafin mutants: full antipeptidase activity is essential to preserve lung tissue integrity in experimental emphysema. Biochem J. 2007;405:455-63 pubmed
    Pre-elafin is a tight-binding inhibitor of neutrophil elastase and myeloblastin; two enzymes thought to contribute to tissue damage in lung emphysema...
  13. Butler M, Robertson I, Greene C, O Neill S, Taggart C, McElvaney N. Elafin prevents lipopolysaccharide-induced AP-1 and NF-kappaB activation via an effect on the ubiquitin-proteasome pathway. J Biol Chem. 2006;281:34730-5 pubmed
    The serine anti-protease elafin is expressed by monocytes, alveolar macrophages, neutrophils, and at mucosal surfaces and possesses antimicrobial activity...
  14. Sallenave J, Marsden M, Ryle A. Isolation of elafin and elastase-specific inhibitor (ESI) from bronchial secretions. Evidence of sequence homology and immunological cross-reactivity. Biol Chem Hoppe Seyler. 1992;373:27-33 pubmed
    ..Gregory, H., Young, J.A. & Christophers, E. (1990) J. Biol. Chem. 265, 14791-14795) which the authors called elafin. We also describe the purification of the complete elafin molecule from bronchial secretions from a patient ..
  15. Schmid M, Fellermann K, Fritz P, Wiedow O, Stange E, Wehkamp J. Attenuated induction of epithelial and leukocyte serine antiproteases elafin and secretory leukocyte protease inhibitor in Crohn's disease. J Leukoc Biol. 2007;81:907-15 pubmed
    b>Elafin (or skin-derived antileukoprotease) and secretory leukocyte protease inhibitor (SLPI) are serine antiproteases antagonizing human neutrophil elastase (HNE), thereby preventing tissue injury from excessive release of proteolytic ..
  16. Bellemare A, Vernoux N, Morisset D, Bourbonnais Y. Human pre-elafin inhibits a Pseudomonas aeruginosa-secreted peptidase and prevents its proliferation in complex media. Antimicrob Agents Chemother. 2008;52:483-90 pubmed
    ..Human pre-elafin, best characterized as a potent inhibitor of neutrophil elastase with anti-inflammatory properties, was also shown ..
  17. Bellemare A, Vernoux N, Morin S, Gagné S, Bourbonnais Y. Structural and antimicrobial properties of human pre-elafin/trappin-2 and derived peptides against Pseudomonas aeruginosa. BMC Microbiol. 2010;10:253 pubmed publisher
    ..The full-length protein as well as the N-terminal "cementoin" and C-terminal "elafin" domains were also shown to possess broad antimicrobial activity, namely ..
  18. Guyot N, Zani M, Maurel M, Dallet Choisy S, Moreau T. Elafin and its precursor trappin-2 still inhibit neutrophil serine proteinases when they are covalently bound to extracellular matrix proteins by tissue transglutaminase. Biochemistry. 2005;44:15610-8 pubmed
    b>Elafin and its precursor trappin-2 (also called pre-elafin) are potent protein inhibitors of neutrophil serine proteases such as leukocyte elastase and proteinase 3...
  19. Henriksen P, Hitt M, Xing Z, Wang J, Haslett C, Riemersma R, et al. Adenoviral gene delivery of elafin and secretory leukocyte protease inhibitor attenuates NF-kappa B-dependent inflammatory responses of human endothelial cells and macrophages to atherogenic stimuli. J Immunol. 2004;172:4535-44 pubmed
    ..To counteract the activity of HNE, we have examined the effects of adenoviral gene delivery of the anti-elastases elafin, previously demonstrated within human atheroma, and murine secretory leukocyte protease inhibitor (SLPI), a ..
  20. Pol A, Pfundt R, Zeeuwen P, Molhuizen H, Schalkwijk J. Transcriptional regulation of the elafin gene in human keratinocytes. J Invest Dermatol. 2003;120:301-7 pubmed
    b>Elafin (also known as skin-derived anti-leukoproteinase/trappin-2) is an epithelial host-defense protein that is absent in normal skin but highly induced in keratinocytes of inflamed skin (e.g...
  21. Simpson A, Wallace W, Marsden M, Govan J, Porteous D, Haslett C, et al. Adenoviral augmentation of elafin protects the lung against acute injury mediated by activated neutrophils and bacterial infection. J Immunol. 2001;167:1778-86 pubmed
    ..We postulated that genetic augmentation of elafin (an endogenous inhibitor of HNE with intrinsic antimicrobial activity) could protect the lung against acute ..
  22. Francart C, Dauchez M, Alix A, Lippens G. Solution structure of R-elafin, a specific inhibitor of elastase. J Mol Biol. 1997;268:666-77 pubmed
    The solution structure of r-elafin, a specific elastase inhibitor, has been determined using NMR spectroscopy...
  23. Sallenave J, Silva A. Characterization and gene sequence of the precursor of elafin, an elastase-specific inhibitor in bronchial secretions. Am J Respir Cell Mol Biol. 1993;8:439-45 pubmed
    ..inhibitors have been identified and characterized: alpha 1-proteinase inhibitor, mucus proteinase inhibitor, and elafin. The elafin molecule, a 6.0 kD inhibitor of serine proteinases shows homology with mucus proteinase inhibitor...
  24. Wiedow O, Schroder J, Gregory H, Young J, Christophers E. Elafin: an elastase-specific inhibitor of human skin. Purification, characterization, and complete amino acid sequence. J Biol Chem. 1990;265:14791-5 pubmed
    ..The high affinity as well as the apparent specificity for elastases suggests a functional role in preventing elastase-mediated tissue proteolysis. It is suggested that the term "elafin" be used to designate this inhibitor.
  25. Saheki T, Ito F, Hagiwara H, Saito Y, Kuroki J, Tachibana S, et al. Primary structure of the human elafin precursor preproelafin deduced from the nucleotide sequence of its gene and the presence of unique repetitive sequences in the prosegment. Biochem Biophys Res Commun. 1992;185:240-5 pubmed
    The human elafin gene was cloned and its entire nucleotide sequence was determined to deduce the amino acid sequence for the precursor of elafin, an elastase-specific inhibitor. The gene spans approximately 1...
  26. Dabić D, Brkljačić L, Tandarić T, Zinić M, Vianello R, Frkanec L, et al. The Metal Effect on Self-Assembling of Oxalamide Gelators Explored by Mass Spectrometry and DFT Calculations. J Am Soc Mass Spectrom. 2018;29:103-113 pubmed publisher
    ..Four bis-oxalamides were chosen to show if electrospray ionization mass spectrometry (ESI-MS) could be used as a prediction of a good gelator and also to shed light on the gelation processes...
  27. Kranawetvogl A, Worek F, Thiermann H, John H. Modification of human serum albumin by the nerve agent VX: microbore liquid chromatography/electrospray ionization high-resolution time-of-flight tandem mass spectrometry method for detection of phosphonylated tyrosine and novel cysteine containing d. Rapid Commun Mass Spectrom. 2016;30:2191-200 pubmed publisher
    ..were separated by microbore liquid chromatography (?LC) online coupled to positive electrospray ionization (ESI) with subsequent high-resolution time-of-flight tandem mass spectrometry (HR MS/MS) allowing identification of ..
  28. Zhu J, Heng Y, Zhang D, Wen Y, Li H, Zhao G. Synthesis and characterization of enzymatically cross-linked feruloyl amylopectin for curcumin encapsulation. Int J Biol Macromol. 2016;85:126-32 pubmed publisher
    ..RP-HPLC-DAD/ESI-TOF-MS measured ferulic acid and its derivatives in FAP and CL-FAP...
  29. Higashi T, Watanabe S, Tomaru K, Yamazaki W, Yoshizawa K, Ogawa S, et al. Unconjugated bile acids in rat brain: Analytical method based on LC/ESI-MS/MS with chemical derivatization and estimation of their origin by comparison to serum levels. Steroids. 2017;125:107-113 pubmed publisher
    ..Based on all the results, we concluded that the BAs found in the brain are mostly derived from the periphery and the major mechanism for the transportation of the unconjugated BAs to the brain is by passive diffusion. ..
  30. Deng M, Zha J, Jiang Z, Jia X, Shi Y, Li P, et al. Apatinib exhibits anti-leukemia activity in preclinical models of acute lymphoblastic leukemia. J Transl Med. 2018;16:47 pubmed publisher
    ..Our study indicates that Apatinib exerts its anti-leukemia effect by inducing apoptosis through suppressing the VEGFR2 signaling pathway, supporting a potential role for Apatinib in the treatment of ALL. ..
  31. Chen B, Long P, Sun Y, Meng Q, Liu X, Cui H, et al. The chemical profiling of loquat leaf extract by HPLC-DAD-ESI-MS and its effects on hyperlipidemia and hyperglycemia in rats induced by a high-fat and fructose diet. Food Funct. 2017;8:687-694 pubmed publisher
    ..The fluorescence data revealed that the LLE binds with ?-amylase and ?-glucosidase. The animal experiment results indicated that the LLE significantly decreased the levels of fasting blood glucose, and hepatic and serum triglycerides. ..
  32. Wu G, Bennett S, Bornman J, Clarke M, Fang Z, Johnson S. Phenolic profile and content of sorghum grains under different irrigation managements. Food Res Int. 2017;97:347-355 pubmed publisher
    ..liquid chromatography coupled with diode array detection and electrospray ionization mass spectrometry (HPLC-DAD-ESI-MS). A total of 25 individual polyphenols were unequivocally or tentatively identified...
  33. Moskovets E, Ivanov A. Comparative studies of peak intensities and chromatographic separation of proteolytic digests, PTMs, and intact proteins obtained by nanoLC-ESI MS analysis at room and elevated temperatures. Anal Bioanal Chem. 2016;408:3953-68 pubmed publisher
    ..to provide conditions for accurate temperature stabilization and temperature homogeneity when performing nanoLC-ESI MS analysis...
  34. Zhuang Z, Yang X, Huang X, Gu H, Wei H, He Y, et al. Three new piscidins from orange-spotted grouper (Epinephelus coioides): Phylogeny, expression and functional characterization. Fish Shellfish Immunol. 2017;66:240-253 pubmed publisher
    ..The peptide ecPis-2S was proved to exist in spleen of orange-spotted grouper by HPLC followed by ESI-LCMS analysis...
  35. Gao Q, Sun J, Xun H, Yao X, Wang J, Tang F. A new azadirachta from the crude extracts of neem (Azadirachta Indica A. Juss) seeds. Nat Prod Res. 2017;31:1739-1746 pubmed publisher
    ..Juss) seeds, which were determined by UV, IR, HR-ESI-MS and NMR data analyses...
  36. Degenkolb T, Götze L, von Döhren H, Vilcinskas A, Brückner H. Sequences of stilboflavin C: towards the peptaibiome of the filamentous fungus Stilbella (= Trichoderma) flavipes. J Pept Sci. 2016;22:517-24 pubmed publisher
    ..HPLC coupled to QqTOF-ESI-HRMS provided the sequences of 10 16-residue peptides and five 19-residue peptides, all of which were N-terminally ..
  37. Dinda M, Mazumdar S, Das S, Ganguly D, Dasgupta U, Dutta A, et al. The Water Fraction of Calendula officinalis Hydroethanol Extract Stimulates In Vitro and In Vivo Proliferation of Dermal Fibroblasts in Wound Healing. Phytother Res. 2016;30:1696-1707 pubmed publisher
    ..officinalis in wound healing. In addition, HPLC-ESI MS analysis of the active water fraction revealed the presence of two major compounds, rutin and quercetin-3-O-..
  38. Li N, Pan Z, Zhang D, Wang H, Yu B, Zhao S, et al. Chemical Components, Biological Activities, and Toxicological Evaluation of the Fruit (Aril) of Two Precious Plant Species from Genus Taxus. Chem Biodivers. 2017;: pubmed publisher
    ..And nineteen compounds were tentatively assigned from the two varieties, via tandem mass spectrometry using a LC-ESI-Q-TOF-MS instrument, which included phenols, flavonoids, and terpenes...
  39. Arba M, Iavarone F, Vincenzoni F, Manconi B, Vento G, Tirone C, et al. Proteomic characterization of the acid-insoluble fraction of whole saliva from preterm human newborns. J Proteomics. 2016;146:48-57 pubmed publisher
    ..three groups were submitted to in-gel tryptic digestion and the peptide mixtures analysed by high resolution HPLC–ESI–MS/MS...
  40. Prabhakara C, Patil S, Kulkarni A, Naik V, Manjunatha M, Kinnal S, et al. Synthesis, spectral, thermal, fluorescence, antimicrobial, anthelmintic and DNA cleavage studies of mononuclear metal chelates of bi-dentate 2H-chromene-2-one Schiff base. J Photochem Photobiol B. 2015;148:322-332 pubmed publisher
    ..characterized based on IR, (1)H NMR, (13)C NMR, UV-visible, ESR, magnetic, thermal, fluorescence, mass and ESI-MS studies. The complexes are completely soluble in DMF and DMSO...
  41. Kou Y, Li L, Li H, Tan Y, Li B, Wang K, et al. Berberine suppressed epithelial mesenchymal transition through cross-talk regulation of PI3K/AKT and RAR?/RAR? in melanoma cells. Biochem Biophys Res Commun. 2016;479:290-296 pubmed publisher
  42. Zhao D, Feng B, Chen S, Chen G, Li Z, Lu X, et al. C21 steroidal glycosides from the roots of Cynanchum paniculatum. Fitoterapia. 2016;113:51-7 pubmed publisher
    ..Kitag. Their structures were elucidated on the basis of 1D- and 2D-NMR spectroscopic data as well as HR-ESI-MS analysis...
  43. Fedorova M, Griesser E, Vemula V, Weber D, Ni Z, Hoffmann R. Protein and lipid carbonylation in cellular model of nitrosative stress: mass spectrometry, biochemistry and microscopy study. Free Radic Biol Med. 2014;75 Suppl 1:S15 pubmed publisher
    ..Lipid carbonylation was quantified by RPC-ESI-MS/MS...
  44. Aiello G, Fasoli E, Boschin G, Lammi C, Zanoni C, Citterio A, et al. Proteomic characterization of hempseed (Cannabis sativa L.). J Proteomics. 2016;147:187-196 pubmed publisher
    ..The experimental approach, based on combinatorial peptide ligand libraries (CPLLs), SDS-PAGE separation, nLC-ESI-MS/MS identification, and database search, permitted identifying in total 181 expressed proteins...
  45. Baranowska I, Hejniak J, Magiera S. Development and validation of a RP-UHPLC-ESI-MS/MS method for the chiral separation and determination of flavanone, naringenin and hesperetin enantiomers. Talanta. 2016;159:181-8 pubmed publisher
    A quick and sensitive RP-UHPLC-ESI-MS/MS method for the separation of flavanone, naringenin and hesperetin enantiomers was developed...
  46. Geyer H, Mesa R. Emerging drugs for the treatment of myelofibrosis. Expert Opin Emerg Drugs. 2015;20:663-78 pubmed publisher
    ..It is anticipated that these agents will be increasingly integrated into standard treatment algorithms for MF symptom management. ..
  47. Yang H, Yao W, Wang Y, Shi L, Su R, Wan D, et al. High-throughput screening of triplex DNA binders from complicated samples by 96-well pate format in conjunction with peak area-fading UHPLC-Orbitrap MS. Analyst. 2017;142:670-675 pubmed publisher
    ..plate (as control) were incubated with ligand libraries, and the unbound ligands were directly determined via UHPLC-ESI-MS...
  48. Wang S, Liu Y, Sun H, DU L, Xu N. [Determination of myclobutanil and difenoconazole residues in pollen and honey of litchi by high performance liquid chromatography-tandem mass spectrometry]. Se Pu. 2018;36:17-22 pubmed publisher
    ..extracts were separated on a Poroshell-120 EC-C18 chromatographic column, the positive electrospray ion (ESI+) source and selected ion monitoring (SIM) mode were used...
  49. Granafei S, Azzone P, Spinelli V, Losito I, Palmisano F, Cataldi T. Hydrophilic interaction and reversed phase mixed-mode liquid chromatography coupled to high resolution tandem mass spectrometry for polar lipids analysis. J Chromatogr A. 2016;1477:47-55 pubmed publisher
    ..Thus a mixture of PC extracted from soybean was characterized by MMC coupled to electrospray ionization (ESI) high-resolution Fourier-transform mass spectrometry (FTMS) using an orbital trap analyzer...
  50. Ham H, Seo J, Yoon H, Shin S. Label-free measurement of the yeast short chain TAG lipase activity by ESI-MS after one-step esterification. J Lipid Res. 2017;58:625-631 pubmed publisher
    ..derivatization method for label-free quantification of a mixture of TAG, DAG, and MAG following lipase assay by ESI-MS...
  51. Ares A, Nozal M, Bernal J. Development and validation of a liquid chromatography-tandem mass spectrometry method to determine intact glucosinolates in bee pollen. J Chromatogr B Analyt Technol Biomed Life Sci. 2015;1000:49-56 pubmed publisher
    ..in bee pollen, by means of liquid chromatography tandem mass spectrometry (LC-MS/MS) with electrospray ionization (ESI)...
  52. Oh M, Consta S. What factors determine the stability of a weak protein-protein interaction in a charged aqueous droplet?. Phys Chem Chem Phys. 2017;: pubmed publisher
    ..detection of the complex association (dissociation) constant by using electrospray ionization mass spectrometry (ESI-MS)...
  53. Eng L, Dawood S, Dent R. Palliative systemic therapy for young women with metastatic breast cancer. Curr Opin Support Palliat Care. 2015;9:301-7 pubmed publisher
    ..Combined palliative and holistic approaches are essential to help young women navigate the marathon of treatment for metastatic breast cancer. ..
  54. Quirino J. Sodium dodecyl sulfate removal during electrospray ionization using cyclodextrins as simple sample solution additive for improved mass spectrometric detection of peptides. Anal Chim Acta. 2018;1005:54-60 pubmed publisher
    ..bottom-up proteomics because SDS affects the quality of the data in electrospray ionization mass spectrometry (ESI-MS)...
  55. Park H, Park S, Hong H, Suh H, Shin K. Structural elucidation of anti-metastatic rhamnogalacturonan II from the pectinase digest of citrus peels (Citrus unshiu). Int J Biol Macromol. 2017;94:161-169 pubmed publisher
    ..on sugar composition, methylation analysis, oligosaccharide analysis, and sequencing using GC, GC-MS, LC-MS, and ESI-MS/MS analyses...
  56. Yu J, Zheng J, Lin J, Jin L, Yu R, Mak S, et al. Indirubin-3-Oxime Prevents H2O2-Induced Neuronal Apoptosis via Concurrently Inhibiting GSK3? and the ERK Pathway. Cell Mol Neurobiol. 2017;37:655-664 pubmed publisher
  57. Li S, Lin Z, Jiang H, Tong L, Wang H, Chen S. Rapid Identification and Assignation of the Active Ingredients in Fufang Banbianlian Injection Using HPLC-DAD-ESI-IT-TOF-MS. J Chromatogr Sci. 2016;54:1225-37 pubmed publisher
    ..detector, and coupled to an electrospray ionization with ion-trap time-of-flight mass spectrometry (HPLC-DAD-ESI-IT-TOF-MS) method, was established for the identification of chemical profile in FBI...
  58. Fitzgerald K, Pelletier L, Reznek M. A Queue-Based Monte Carlo Analysis to Support Decision Making for Implementation of an Emergency Department Fast Track. J Healthc Eng. 2017;2017:6536523 pubmed publisher
    ..the ED occupancy and nursing resource demand were modeled and analyzed using the Emergency Severity Index (ESI) levels of patients, rather than the number of beds in the department...
  59. Acar N, Berdeaux O, Grégoire S, Cabaret S, Martine L, Gain P, et al. Lipid composition of the human eye: are red blood cells a good mirror of retinal and optic nerve fatty acids?. PLoS ONE. 2012;7:e35102 pubmed publisher
    ..in gas chromatography and liquid chromatography coupled to an electrospray ionization source-mass spectrometer (LC-ESI-MS)...
  60. Wang L, Gan C, Wang Z, Liu L, Gao M, Li Q, et al. Determination and Pharmacokinetic Study of Three Diterpenes in Rat Plasma by UHPLC-ESI-MS/MS after Oral Administration of Rosmarinus officinalis L. Extract. Molecules. 2017;22: pubmed publisher
    ..officinalis. An Ultra High Performance Liquid Chromatography-Tandem Mass Spectrometry (UHPLC-ESI-MS/MS) method was developed for the determination of carnosol, rosmanol, and carnosic acid isolated from R...
  61. Hashim S, Schwarz L, Danylec B, Potdar M, Boysen R, Hearn M. Selectivity mapping of the binding sites of (E)-resveratrol imprinted polymers using structurally diverse polyphenolic compounds present in Pinot noir grape skins. Talanta. 2016;161:425-436 pubmed publisher
    ..high-performance liquid chromatography (RP-HPLC) and electrospray ionisation tandem mass spectrometry (ESI MS/MS) was then employed for compound analysis and identification...
  62. Jeong Y, Oh Y, Cho W, Yang H, Ma J. Maydis Stigma Elicits Analgesia and Blocks Edema in Mice and Inhibits Inflammation in Macrophages. Am J Chin Med. 2017;45:1477-1496 pubmed publisher
    ..Finally, we investigated the components of MSE using UPLC-ESI-MS and found that it contains the maysin as a marker component...
  63. Abruzzo A, Giordani B, Parolin C, Vitali B, Protti M, Mercolini L, et al. Novel mixed vesicles containing lactobacilli biosurfactant for vaginal delivery of an anti-Candida agent. Eur J Pharm Sci. 2018;112:95-101 pubmed publisher
    ..BC9, isolated from the vagina of a healthy premenopausal woman, and was chemically characterized by FT-IR and ESI-MS...
  64. Ma Y, Li J, Huang W, Wang Z, Xiao W, Song Y, et al. [Simultaneous determination of six triterpenoid acids from Guizhi Fuling capsules by UPLC-MS/MS]. Zhongguo Zhong Yao Za Zhi. 2017;42:3368-3373 pubmed publisher
    ..The samples were analyzed by tandem mass spectrometer with negative electrospray ionization (ESI) source, and monitored under a multiple reaction monitoring (MRM) mode, with the quantitative ion pairs m/z 527...
  65. Roghanian A, Williams S, Sheldrake T, Brown T, Oberheim K, Xing Z, et al. The antimicrobial/elastase inhibitor elafin regulates lung dendritic cells and adaptive immunity. Am J Respir Cell Mol Biol. 2006;34:634-42 pubmed
    ..We have previously shown in acute models that elafin (a lung natural chemotactic molecule for macrophages and neutrophils, with potent antimicrobial and neutrophil ..
  66. López Muňoz M, Daniele A, Zorzi M, Medana C, Calza P. Investigation of the photocatalytic transformation of acesulfame K in the presence of different TiO2-based materials. Chemosphere. 2018;193:151-159 pubmed publisher
    ..high-performance liquid chromatography (HPLC) coupled with a LTQ-Orbitrap analyzer via an electrospray ionization (ESI) in the negative ion mode...
  67. Qin Q, Meng T, Tan M, Liu Y, Luo X, Zou B, et al. Synthesis, crystal structure and biological evaluation of a new dasatinib copper(II) complex as telomerase inhibitor. Eur J Med Chem. 2018;143:1597-1603 pubmed publisher
    A new copper(II) complex of dasatinib (DAS) was synthesized and characterized via ESI-MS, UV-Vis, IR, single-crystal X-ray diffraction analysis, 1H and 13C NMR spectroscopy, and elemental analysis...
  68. Krizova I, Schultz J, Nemec I, Cabala R, Hynek R, Kuckova S. Comparison of analytical tools appropriate for identification of proteinaceous additives in historical mortars. Anal Bioanal Chem. 2018;410:189-200 pubmed publisher
    ..MALDI-TOF MS), liquid chromatography-electrospray ionisation-quadrupole-time of flight mass spectrometry (LC-ESI-Q-TOF MS) and enzyme-linked immunosorbent assay (ELISA)...
  69. Jiang T, Wang X, Ding C, Du X. Genistein attenuates isoflurane-induced neurotoxicity and improves impaired spatial learning and memory by regulating cAMP/CREB and BDNF-TrkB-PI3K/Akt signaling. Korean J Physiol Pharmacol. 2017;21:579-589 pubmed publisher
    ..These observations suggest that genistein exerts neuroprotective effects by suppressing isoflurane-induced neuronal apoptosis and by activating cAMP/CREB-BDNF-TrkB-PI3/Akt signaling...
  70. Gondoin A, Hampe C, Eudes R, Fayolle C, Pierre Eugène C, Miteva M, et al. Identification of insulin-sensitizing molecules acting by disrupting the interaction between the Insulin Receptor and Grb14. Sci Rep. 2017;7:16901 pubmed publisher
    ..The use of C8 as a lead compound should allow for the development of new molecules of potential therapeutic interest for the treatment of diabetes. ..
  71. Han J, Wanrooij J, van Bommel M, Quye A. Characterisation of chemical components for identifying historical Chinese textile dyes by ultra high performance liquid chromatography - photodiode array - electrospray ionisation mass spectrometer. J Chromatogr A. 2017;1479:87-96 pubmed publisher
    ..UHPLC) coupled to both Photodiode Array detection (PDA) and Electrospray Ionisation Mass Spectrometer (ESI-MS) to the chemical characterisation of common textile dyes in ancient China...
  72. Shaheen N, Lu Y, Geng P, Shao Q, Wei Y. Isolation of four phenolic compounds from Mangifera indica L. flowers by using normal phase combined with elution extrusion two-step high speed countercurrent chromatography. J Chromatogr B Analyt Technol Biomed Life Sci. 2017;1046:211-217 pubmed publisher
    ..7mg were separated well with high purities of 98.68% and 99.71%, respectively. The separated phenolic compounds were identified and confirmed by HPLC, UPLC-QTOF/ESI-MS, 1H and 13C NMR spectrometric analysis.
  73. Huang W, Yi X, Feng J, Wang Y, He X. Piperidine alkaloids from Alocasia macrorrhiza. Phytochemistry. 2017;143:81-86 pubmed publisher
    ..Schott. Their structures were elucidated based on 1D and 2D NMR, IR, HR-ESI-MS spectroscopic analysis and the application of a modified Mosher method...
  74. Kang Y, Schneider B, Covey T. On the Nature of Mass Spectrometer Analyzer Contamination. J Am Soc Mass Spectrom. 2017;28:2384-2392 pubmed publisher
    Sample throughput in electrospray ionization mass spectrometry (ESI-MS) is limited by the need for frequent ion path cleaning to remove accumulated debris that can lead to charging and general performance degradation...
  75. Jiang L, Zhang C, Li H. Quantification of ?-eudesmol in rat plasma using LC-MS/MS and its application to a pharmacokinetic study. Biomed Chromatogr. 2017;31: pubmed publisher
    ..5:22.5:0.1, v/v/v) as the mobile phase at a flow rate of 0.4?mL/min. An ESI source was applied and operated in positive ion mode; a selected reaction monitoring scan was used for ..
  76. Klima M, Altenburger M, Kempf J, Auwärter V, Neukamm M. Determination of medicinal and illicit drugs in post mortem dental hard tissues and comparison with analytical results for body fluids and hair samples. Forensic Sci Int. 2016;265:166-71 pubmed publisher
    ..The extracts were screened for drugs by LC-MS(n) (ToxTyperâ„¢) and quantitatively analyzed with LC-ESI-MS/MS in MRM mode...
  77. Harnedy P, O Keeffe M, FitzGerald R. Fractionation and identification of antioxidant peptides from an enzymatically hydrolysed Palmaria palmata protein isolate. Food Res Int. 2017;100:416-422 pubmed publisher
    ..The most active SP-RP-HPLC peptide fraction (SP-RP-HPLC-30-F26) was analysed by ESI-MS/MS. Seventeen novel peptide sequences were identified in this fraction...
  78. Liu C, Wang W. Non-ignorable missingness item response theory models for choice effects in examinee-selected items. Br J Math Stat Psychol. 2017;70:499-524 pubmed publisher
    Examinee-selected item (ESI) design, in which examinees are required to respond to a fixed number of items in a given set, always yields incomplete data (i.e...
  79. Chae J, Jang H, Kim C. Sequential detection of Fe3+/2+ and pyrophosphate by a colorimetric chemosensor in a near-perfect aqueous solution. Photochem Photobiol Sci. 2017;16:1812-1820 pubmed publisher
    ..Based on UV-vis titrations, Job plot and ESI-mass spectrometry analyses, the sensing mechanisms of Fe3+, Fe2+ and PPi were proposed.
  80. Marischen L, Wesch D, Schroder J, Wiedow O, Kabelitz D. Human gammadelta T cells produce the protease inhibitor and antimicrobial peptide elafin. Scand J Immunol. 2009;70:547-52 pubmed publisher
    ..While alpha-defensins were absent and beta-defensins (HBD1) present only in rare gammadelta T cell clones, elafin mRNA was induced by supernatant of Pseudomonas aeruginosa grown under static conditions...
  81. Qin M, Chai S, Ma Y, Gao H, Zhang H, He Q. [Determination of pyriminobac-methyl and bispyribac-sodium residues in rice by liquid chromatography-tandem mass spectrometry based on QuEChERS]. Se Pu. 2017;35:719-723 pubmed publisher
    ..Positive electrospray ionization (ESI+) was used...
  82. Li Y, Wang L, Zhu X, Gao Y, Chen J. [Determination of hexabromocyclododecanes in ambient air by high performance liquid chromatography- electrospray ionization-mass spectrometry]. Se Pu. 2017;35:1080-1085 pubmed publisher
    A high performance liquid chromatography-electrospray ionization-mass spectrometry (HPLC-ESI-MS) method was developed for the determination of hexabromocyclododecanes (HBCDs) in ambient air samples...
  83. Dong X, Zhang Y, Dong J, Zhao Y, Guo J, Wang Z, et al. Urinary metabolomic profiling in rats exposed to dietary di(2-ethylhexyl) phthalate (DEHP) using ultra-performance liquid chromatography quadrupole time-of-flight tandem mass spectrometry (UPLC/Q-TOF-MS). Environ Sci Pollut Res Int. 2017;24:16659-16672 pubmed publisher
    ..The clusters in the positive electrospray ionization (ESI) mode were xanthurenic acid, kynurenic acid, nonate, N6-methyladenosine, and L-isoleucyl-L-proline...
  84. Jespers V, Kyongo J, Joseph S, Hardy L, Cools P, Crucitti T, et al. A longitudinal analysis of the vaginal microbiota and vaginal immune mediators in women from sub-Saharan Africa. Sci Rep. 2017;7:11974 pubmed publisher
    ..incident visit and when concentrations of proinflammatory cytokines (IL-1?, IL-12p70) were increased and IP-10 and elafin were decreased...
  85. Haahr M, Azawi N, Andersen L, Carlson S, Lund L. A Retrospective Study of Erectile Function and Use of Erectile Aids in Prostate Cancer Patients After Radical Prostatectomy in Denmark. Sex Med. 2017;5:e156-e162 pubmed publisher
    ..Recovery was defined as self-reported erection sufficient for intercourse (ESI) with or without usage of erectile aids...
  86. Bravin C, Badetti E, Puttreddy R, Pan F, Rissanen K, Licini G, et al. Binding Profiles of Self-Assembled Supramolecular Cages from ESI-MS Based Methodology. Chemistry. 2017;: pubmed publisher
    ..Herein, we report a rapid method based on ESI-MS for the determination of binding profiles for linear saturated dicarboxylic acids within a series of different ..
  87. Chai H, Yan Z, Huang K, Jiang Y, Zhang L. MicroRNA expression, target genes, and signaling pathways in infants with a ventricular septal defect. Mol Cell Biochem. 2018;439:171-187 pubmed publisher
    ..These effects could also be exerted via the upregulation of eight specific target genes, the subsequent over-activation of the PKC and PI3 K-Akt pathways, and the eventual abnormal cardiac development and VSD. ..
  88. Chen G, Jirjees F, Al Bawab A, McElnay J. Quantification of amlodipine in dried blood spot samples by high performance liquid chromatography tandem mass spectrometry. J Chromatogr B Analyt Technol Biomed Life Sci. 2018;1072:252-258 pubmed publisher
    ..was quantified using a Waters Quattro Premier mass spectrometer coupled with an electro-spray ionization (ESI) source in positive ion mode. The MRM transitions of 408.9 m/z→238.1m/z and 408.9→294...
  89. Tejera P, Wang Z, Zhai R, Su L, Sheu C, Taylor D, et al. Genetic polymorphisms of peptidase inhibitor 3 (elafin) are associated with acute respiratory distress syndrome. Am J Respir Cell Mol Biol. 2009;41:696-704 pubmed publisher
    Peptidase inhibitor 3 (PI3, elafin) is a protease inhibitor produced locally in the lung, where it plays a central role in controlling excessive activity of neutrophil elastase...
  90. Zhao D, Le T, Nielsen S, Larsen L. Effect of Storage on Lactase-Treated ?-Casein and ?-Lactoglobulin with Respect to Bitter Peptide Formation and Subsequent in Vitro Digestibility. J Agric Food Chem. 2017;65:8409-8417 pubmed publisher
    ..Peptidomic analysis by LC-ESI-MS/MS revealed that Ile, Leu, Tyr, and Phe residues near the C-terminus of ?-casein were the main sites of cleavage ..
  91. Young L, Tu L, Raleigh D, Ashcroft A, Radford S. Understanding co-polymerization in amyloid formation by direct observation of mixed oligomers. Chem Sci. 2017;8:5030-5040 pubmed publisher
    ..Electrospray ionization-ion mobility spectrometry-mass spectrometry (ESI-IMS-MS) is uniquely suited to the study of these heterogeneous ensembles...
  92. Sorber J, Levy D, Schwartz A. Pneumocephalus and seizures following epidural steroid injection. Am J Emerg Med. 2017;35:1987.e1-1987.e2 pubmed publisher
    We describe a patient with pneumocephalus following an epidural steroid injection (ESI) who presented with altered mental status, headache, focal neurologic findings and seizures. Pneumocephalus has rarely been described following ESI...
  93. Mahanti B, González Miera G, Martínez Castro E, Bedin M, Martín Matute B, Ott S, et al. Homogeneous Water Oxidation by Half-Sandwich Iridium(III) N-Heterocyclic Carbene Complexes with Pendant Hydroxy and Amino Groups. ChemSusChem. 2017;10:4616-4623 pubmed publisher
    ..are presumably involved in the rate-determining step for water oxidation is established by UV/Vis spectroscopy and ESI-MS under turnover conditions...
  94. Chai W, Lin M, Wang Y, Xu K, Huang W, Pan D, et al. Inhibition of tyrosinase by cherimoya pericarp proanthocyanidins: Structural characterization, inhibitory activity and mechanism. Food Res Int. 2017;100:731-739 pubmed publisher
    In this study, the structure of proanthocyanidins purified from cherimoya (Annona squamosa) pericarp was analyzed by ESI-QTOF-MS and HPLC analyses...
  95. Inthavong C, Hommet F, Bordet F, Rigourd V, Guérin T, Dragacci S. Simultaneous liquid chromatography-tandem mass spectrometry analysis of brominated flame retardants (tetrabromobisphenol A and hexabromocyclododecane diastereoisomers) in French breast milk. Chemosphere. 2017;186:762-769 pubmed publisher
    ..This study describes the development and optimisation of a rapid LC-ESI-MS/MS method using isotopic dilution quantification including a simplified extraction step using a mixture of ..
  96. Li H, Yuan F, Luo Y, Wang J, Zhang C, Zhou W, et al. Biosynthesis of staphylococcal enterotoxin A by genetic engineering technology and determination of staphylococcal enterotoxin A in water by HPLC-ESI-TOF. Environ Sci Pollut Res Int. 2017;: pubmed publisher
    ..aureus, SEA was analyzed by HPLC-ESI-TOF...
  97. Christopeit T, Albert A, Leiros H. Discovery of a novel covalent non-?-lactam inhibitor of the metallo-?-lactamase NDM-1. Bioorg Med Chem. 2016;24:2947-2953 pubmed publisher
    ..Electrospray ionization-mass spectrometry (ESI-MS) and single site directed mutagenesis were used to show that the inhibitor forms a covalent bond with Lys224 in ..
  98. Limonier F, Van Steendam K, Waeterloos G, Brusselmans K, Sneyers M, Deforce D. An application of mass spectrometry for quality control of biologicals: Highly sensitive profiling of plasma residuals in human plasma-derived immunoglobulin. J Proteomics. 2017;152:312-320 pubmed publisher
    ..This study aims to consider LC-ESI-QTOF data-dependent acquisition in combination with sample fractionation for this purpose...