EIF5A

Summary

Gene Symbol: EIF5A
Description: eukaryotic translation initiation factor 5A
Alias: EIF-5A, EIF5A1, eIF5AI, eukaryotic translation initiation factor 5A-1, eIF-4D, eIF-5A-1, eIF-5A1, eukaryotic initiation factor 5A, rev-binding factor
Species: human
Products:     EIF5A

Top Publications

  1. Bevec D, Hauber J. Eukaryotic initiation factor 5A activity and HIV-1 Rev function. Biol Signals. 1997;6:124-33 pubmed
    b>Eukaryotic initiation factor 5A (eIF-5A) is the only cellular protein known to contain the unusual amino acid hypusine, a modification that appears to be required for cell proliferation...
  2. Hofmann W, Reichart B, Ewald A, Muller E, Schmitt I, Stauber R, et al. Cofactor requirements for nuclear export of Rev response element (RRE)- and constitutive transport element (CTE)-containing retroviral RNAs. An unexpected role for actin. J Cell Biol. 2001;152:895-910 pubmed
    ..We show that eukaryotic initiation factor 5A (eIF-5A) is essential for Rev and Rev-mediated viral RNA export, but not for nuclear export of CTE ..
  3. Klier H, Csonga R, Joao H, Eckerskorn C, Auer M, Lottspeich F, et al. Isolation and structural characterization of different isoforms of the hypusine-containing protein eIF-5A from HeLa cells. Biochemistry. 1995;34:14693-702 pubmed
    Posttranslational modification of a specific lysine residue in eukaryotic initiation factor 5A (eIF-5A) is essential for cell viability and proliferation. The product of this modification is hypusine, an amino acid unique to eIF-5A...
  4. Lee Y, Joe Y, Wolff E, Dimitriadis E, Park M. Complex formation between deoxyhypusine synthase and its protein substrate, the eukaryotic translation initiation factor 5A (eIF5A) precursor. Biochem J. 1999;340 ( Pt 1):273-81 pubmed
    ..of hypusine [Nepsilon-(4-amino-2-hydroxybutyl) lysine] in a single cellular protein, the precursor of eukaryotic initiation factor 5A (eIF5A). Deoxyhypusine synthase exists as a tetramer with four potential active sites...
  5. Rosorius O, Reichart B, Kratzer F, Heger P, Dabauvalle M, Hauber J. Nuclear pore localization and nucleocytoplasmic transport of eIF-5A: evidence for direct interaction with the export receptor CRM1. J Cell Sci. 1999;112 ( Pt 14):2369-80 pubmed
    b>Eukaryotic initiation factor 5A (eIF-5A) is the only cellular protein known to contain the unusual amino acid hypusine. The exact in vivo function of eIF-5A, however, is to date unknown...
  6. Clement P, Henderson C, Jenkins Z, Smit McBride Z, Wolff E, Hershey J, et al. Identification and characterization of eukaryotic initiation factor 5A-2. Eur J Biochem. 2003;270:4254-63 pubmed
    The phylogenetically conserved eukaryotic translation initiation factor 5A (eIF5A) is the only known cellular protein to contain the post-translationally derived amino acid hypusine [Nepsilon-(4-amino-2-hydroxybutyl)lysine]...
  7. Clement P, Johansson H, Wolff E, Park M. Differential expression of eIF5A-1 and eIF5A-2 in human cancer cells. FEBS J. 2006;273:1102-14 pubmed
    Eukaryotic translation initiation factor 5A (eIF5A) is the only cellular protein that contains the unusual amino acid hypusine [N(epsilon)-(4-amino-2-hydroxybutyl)lysine]...
  8. Kang K, Kim Y, Wolff E, Park M. Specificity of the deoxyhypusine hydroxylase-eukaryotic translation initiation factor (eIF5A) interaction: identification of amino acid residues of the enzyme required for binding of its substrate, deoxyhypusine-containing eIF5A. J Biol Chem. 2007;282:8300-8 pubmed
    ..of hypusine (Nepsilon-(4-amino-2-hydroxybutyl)lysine) in the eukaryotic translation initiation factor 5A (eIF5A)...
  9. Zanelli C, Valentini S. Is there a role for eIF5A in translation?. Amino Acids. 2007;33:351-8 pubmed
    The putative translation factor eIF5A is essential for cell viability and is highly conserved from archaebacteria to mammals...

More Information

Publications130 found, 100 shown here

  1. Cano V, Jeon G, Johansson H, Henderson C, Park J, Valentini S, et al. Mutational analyses of human eIF5A-1--identification of amino acid residues critical for eIF5A activity and hypusine modification. FEBS J. 2008;275:44-58 pubmed
    The eukaryotic translation initiation factor 5A (eIF5A) is the only protein that contains hypusine [Nepsilon-(4-amino-2-hydroxybutyl)lysine], which is required for its activity...
  2. Liu Y, Nemeroff M, Yan Y, Chen K. Interaction of eukaryotic initiation factor 5A with the human immunodeficiency virus type 1 Rev response element RNA and U6 snRNA requires deoxyhypusine or hypusine modification. Biol Signals. 1997;6:166-74 pubmed
    Hypusine formation on the eukaryotic initiation factor 5A (eIF-5A) precursor represents a unique posttranslational modification that is ubiquitously present in eukaryotic cells and archaebacteria...
  3. Lipowsky G, Bischoff F, Schwarzmaier P, Kraft R, Kostka S, Hartmann E, et al. Exportin 4: a mediator of a novel nuclear export pathway in higher eukaryotes. EMBO J. 2000;19:4362-71 pubmed
    ..The export signal in eIF-5A appears to be complex and to involve the hypusine modification that is unique to eIF-5A. We discuss possible cellular roles for nuclear export of eIF-5A. ..
  4. Jenkins Z, Hååg P, Johansson H. Human eIF5A2 on chromosome 3q25-q27 is a phylogenetically conserved vertebrate variant of eukaryotic translation initiation factor 5A with tissue-specific expression. Genomics. 2001;71:101-9 pubmed
    ..and colorectal adenocarcinoma and at moderate levels in the brain, in contrast to the ubiquitously expressed EIF5A1 gene...
  5. Bevec D, Jaksche H, Oft M, Wohl T, Himmelspach M, Pacher A, et al. Inhibition of HIV-1 replication in lymphocytes by mutants of the Rev cofactor eIF-5A. Science. 1996;271:1858-60 pubmed
    b>Eukaryotic initiation factor 5A(eIF-5A) is a cellular cofactor require d for the function of the human immunodeficiency virus type-1 (HIV-1) Rev trans-activator protein...
  6. Schatz O, Oft M, Dascher C, Schebesta M, Rosorius O, Jaksche H, et al. Interaction of the HIV-1 rev cofactor eukaryotic initiation factor 5A with ribosomal protein L5. Proc Natl Acad Sci U S A. 1998;95:1607-12 pubmed
    It has previously been shown that interaction of eukaryotic initiation factor 5A (eIF-5A) with the Rev trans-activator protein of HIV-1 mediates the transport of unspliced or incompletely spliced viral mRNAs across the nuclear envelope...
  7. Li A, Li H, Jin B, Ye Q, Zhou T, Yu X, et al. A novel eIF5A complex functions as a regulator of p53 and p53-dependent apoptosis. J Biol Chem. 2004;279:49251-8 pubmed
    Although eukaryotic translation initiation factor 5A (eIF5A) was originally designated as an "initiation factor," recent data have shown it to be also involved in apoptosis...
  8. Balabanov S, Gontarewicz A, Ziegler P, Hartmann U, Kammer W, Copland M, et al. Hypusination of eukaryotic initiation factor 5A (eIF5A): a novel therapeutic target in BCR-ABL-positive leukemias identified by a proteomics approach. Blood. 2007;109:1701-11 pubmed
    ..Among the 19 proteins found to be differentially expressed, we detected the down-regulation of eukaryotic initiation factor 5A (eIF5A), a protein essential for cell proliferation...
  9. Taylor C, Sun Z, Cliche D, Ming H, Eshaque B, Jin S, et al. Eukaryotic translation initiation factor 5A induces apoptosis in colon cancer cells and associates with the nucleus in response to tumour necrosis factor alpha signalling. Exp Cell Res. 2007;313:437-49 pubmed
    Eukaryotic translation initiation factor 5A (eIF5A) is thought to function as a nucleocytoplasmic shuttle protein...
  10. Ruhl M, Himmelspach M, Bahr G, Hammerschmid F, Jaksche H, Wolff B, et al. Eukaryotic initiation factor 5A is a cellular target of the human immunodeficiency virus type 1 Rev activation domain mediating trans-activation. J Cell Biol. 1993;123:1309-20 pubmed
    ..Using cross-linking experiments and Biospecific Interaction Analysis (BIA) we identify eukaryotic initiation factor 5A (eIF-5A) as a cellular factor binding specifically to the HIV-1 Rev activation domain...
  11. Park J, Aravind L, Wolff E, Kaevel J, Kim Y, Park M. Molecular cloning, expression, and structural prediction of deoxyhypusine hydroxylase: a HEAT-repeat-containing metalloenzyme. Proc Natl Acad Sci U S A. 2006;103:51-6 pubmed
    The eukaryotic initiation factor 5A (eIF5A), a factor essential for eukaryotic cell proliferation, is the only cellular protein containing the polyamine-derived amino acid hypusine [N(epsilon)-(4-amino-2-hydroxybutyl)lysine]...
  12. Park M. The post-translational synthesis of a polyamine-derived amino acid, hypusine, in the eukaryotic translation initiation factor 5A (eIF5A). J Biochem. 2006;139:161-9 pubmed
    The eukaryotic translation initiation factor 5A (eIF5A) is the only cellular protein that contains the unique polyamine-derived amino acid, hypusine [Nepsilon-(4-amino-2-hydroxybutyl)lysine]...
  13. Schrader R, Young C, Kozian D, Hoffmann R, Lottspeich F. Temperature-sensitive eIF5A mutant accumulates transcripts targeted to the nonsense-mediated decay pathway. J Biol Chem. 2006;281:35336-46 pubmed
    ..To gain more insight into these functions temperature-sensitive mutants of the human EIF5A1 were characterized in the yeast Saccharomyces cerevisiae...
  14. Sun Z, Cheng Z, Taylor C, McConkey B, Thompson J. Apoptosis induction by eIF5A1 involves activation of the intrinsic mitochondrial pathway. J Cell Physiol. 2010;223:798-809 pubmed publisher
    The regulatory role of eukaryotic translation initiation factor 5A1 (eIF5A1) in apoptosis was examined using HT-29 and HeLa S3 cells...
  15. Flynn A, Hogarty M. Myc, Oncogenic Protein Translation, and the Role of Polyamines. Med Sci (Basel). 2018;6: pubmed publisher
    ..pharmacologic targeting of components of the protein synthetic machinery, such as the polyamines themselves, the eukaryotic translation initiation factor 4F (eIF4F) complex, and the eukaryotic translation initiation factor 5A (eIF5A).
  16. Pegg A. Unique Characteristics of the Parasite Polyamine Pathway. Structure. 2018;26:1427-1429 pubmed publisher
    ..is in the formation of hypusine, an essential post-translational modification of eukaryotic initiation factor eIF5A. In this issue of Structure, Afandor et al...
  17. Grüber G. The Beauty of a Visualized Peroxo-diiron(III) Intermediate. Structure. 2015;23:805-806 pubmed publisher
    ..In this issue, Han et al. use X-ray crystallograpy and UV/Vis and Mössbauer spectroscopy to provide insights into the fundamental mechanism of the hypusination of eIF-5A and a peroxo intermediate state. ..
  18. Mastracci T, Robertson M, Mirmira R, Anderson R. Polyamine biosynthesis is critical for growth and differentiation of the pancreas. Sci Rep. 2015;5:13269 pubmed publisher
    ..is largely mediated through a requirement for spermidine for the downstream posttranslational modification of eIF5A by its enzymatic activator DHS, which in turn impacts mRNA translation...
  19. Liu Q, Du G, Zhu Z, Zhang C, Sun X, Liu J, et al. Identification of apoptosis-related microRNAs and their target genes in myocardial infarction post-transplantation with skeletal myoblasts. J Transl Med. 2015;13:270 pubmed publisher
    ..Furthermore, we found seven genes including Angptl4, Dpep1, Egr1, Eif5a, Tsc22d3, Irs2 and Cebpb that showed a linear correlation with which miRNAs...
  20. Alcolea P, Alonso A, García Tabares F, Toraño A, Larraga V. An Insight into the proteome of Crithidia fasciculata choanomastigotes as a comparative approach to axenic growth, peanut lectin agglutination and differentiation of Leishmania spp. promastigotes. PLoS ONE. 2014;9:e113837 pubmed publisher
    ..subpopulation over-expresses the whole machinery for maintenance of redox homeostasis and the translation factors eIF5a, EF1α and EF2, what suggests a relationship between the lack of agglutination and a differentiation process.
  21. Kim D, Choi S, Yeom Y, Min S, Kim I. Genome-scale functional analysis of the human genes modulating p53 activity by regulating MDM2 expression in a p53-independent manner. Biochem Biophys Res Commun. 2016;478:976-81 pubmed publisher
    ..Among them, six clones, including NTRK2, GNA15, SFRS2, EIF5A, ELAVL1, and YWHAB mediated MAPK signaling for expressing MDM2...
  22. Kasari V, Margus T, Atkinson G, Johansson M, Hauryliuk V. Ribosome profiling analysis of eEF3-depleted Saccharomyces cerevisiae. Sci Rep. 2019;9:3037 pubmed publisher
    ..Surprisingly, depletion of eEF3 leads to a relative decrease in P-site proline stalling, which we hypothesise is a secondary effect of generally decreased translation and/or decreased competition for the E-site with eIF5A.
  23. Lee W, Kang J, Choi W, Zhang Q, Kim C, Choi U, et al. Mincle-mediated translational regulation is required for strong nitric oxide production and inflammation resolution. Nat Commun. 2016;7:11322 pubmed publisher
    ..transcription, Mincle enhanced the translation of key genes required for nitric oxide synthesis through p38 and eIF5A hypusination, leading to granuloma resolution...
  24. Ivanov I, Shin B, Loughran G, Tzani I, Young Baird S, Cao C, et al. Polyamine Control of Translation Elongation Regulates Start Site Selection on Antizyme Inhibitor mRNA via Ribosome Queuing. Mol Cell. 2018;70:254-264.e6 pubmed publisher
    ..Finally, we identify the translation factor eIF5A as a sensor and effector for polyamine control of uCC translation...
  25. Dever T, Ivanov I. Roles of polyamines in translation. J Biol Chem. 2018;293:18719-18729 pubmed publisher
    ..serves as a precursor for the essential post-translational modification of the eukaryotic translation factor 5A (eIF5A), which is required for synthesis of proteins containing problematic amino acid sequence motifs, including ..
  26. Nishiki Y, Adewola A, Hatanaka M, Templin A, Maier B, Mirmira R. Translational control of inducible nitric oxide synthase by p38 MAPK in islet β-cells. Mol Endocrinol. 2013;27:336-49 pubmed publisher
    ..or inhibition of Nos2 mRNA translation with GC7 [an inhibitor of eukaryotic translation initiation factor 5A (eIF5A) activity] reversed these defects, as did inhibition of p38 MAPK by PD169316...
  27. He H, Deng K, Siddiq M, Pyie A, Mellado W, Hannila S, et al. Cyclic AMP and Polyamines Overcome Inhibition by Myelin-Associated Glycoprotein through eIF5A-Mediated Increases in p35 Expression and Activation of Cdk5. J Neurosci. 2016;36:3079-91 pubmed publisher
    ..Last, we show that putrescine upregulates p35 expression by serving as a substrate for hypusine modification of eIF5A, and that this hypusination is necessary for putrescine's ability to overcome inhibition by MAG...
  28. Bassani F, Romagnoli A, Cacciamani T, Amici A, Benelli D, Londei P, et al. Modification of translation factor aIF5A from Sulfolobus solfataricus. Extremophiles. 2018;22:769-780 pubmed publisher
    Eukaryotic eIF5A and its bacterial orthologue EF-P are translation elongation factors whose task is to rescue ribosomes from stalling during the synthesis of proteins bearing particular sequences such as polyproline stretches...
  29. Bevec D, Klier H, Holter W, Tschachler E, Valent P, Lottspeich F, et al. Induced gene expression of the hypusine-containing protein eukaryotic initiation factor 5A in activated human T lymphocytes. Proc Natl Acad Sci U S A. 1994;91:10829-33 pubmed
    The hypusine-containing protein eukaryotic initiation factor 5A (eIF-5A) is a cellular cofactor critically required for the function of the Rev transactivator protein of human immunodeficiency virus type 1 (HIV-1)...
  30. Yu L, Zhao Y, Quan C, Ji W, Zhu J, Huang Y, et al. Gemcitabine eliminates double minute chromosomes from human ovarian cancer cells. PLoS ONE. 2013;8:e71988 pubmed publisher
    ..Together, our results suggest that gemcitabine is effective in decreasing double minute chromosomes and this affects the biology of ovarian cancer cells. ..
  31. Lozier A, Rich M, Grawe A, Peck A, Zhao P, Chang A, et al. Targeting ornithine decarboxylase reverses the LIN28/Let-7 axis and inhibits glycolytic metabolism in neuroblastoma. Oncotarget. 2015;6:196-206 pubmed
  32. Nguyen S, Leija C, Kinch L, Regmi S, Li Q, Grishin N, et al. Deoxyhypusine Modification of Eukaryotic Translation Initiation Factor 5A (eIF5A) Is Essential for Trypanosoma brucei Growth and for Expression of Polyprolyl-containing Proteins. J Biol Chem. 2015;290:19987-98 pubmed publisher
    ..cofactor called trypanothione and for deoxyhypusine modification of eukaryotic translation initiation factor 5A (eIF5A). eIF5A promotes translation of proteins containing polyprolyl tracts in mammals and yeast...
  33. Liu J, Henao Mejia J, Liu H, Zhao Y, He J. Translational regulation of HIV-1 replication by HIV-1 Rev cellular cofactors Sam68, eIF5A, hRIP, and DDX3. J Neuroimmune Pharmacol. 2011;6:308-21 pubmed publisher
    ..RNA from the nucleus to the cytoplasm; it requires interaction with several cellular cofactors such as Sam68, eIF5A, hRIP, and DDX3...
  34. Nishimura K, Lee S, Park J, Park M. Essential role of eIF5A-1 and deoxyhypusine synthase in mouse embryonic development. Amino Acids. 2012;42:703-10 pubmed publisher
    The eukaryotic initiation factor 5A (eIF5A) contains a polyamine-derived amino acid, hypusine [N(ε)-(4-amino-2-hydroxybutyl)lysine]...
  35. Xu G, Yu H, Shi X, Sun L, Zhou Q, Zheng D, et al. Cisplatin sensitivity is enhanced in non-small cell lung cancer cells by regulating epithelial-mesenchymal transition through inhibition of eukaryotic translation initiation factor 5A2. BMC Pulm Med. 2014;14:174 pubmed publisher
    ..Recent studies have suggested eIF5A-2 may function as a proliferation-related oncogene in tumorigenic processes...
  36. Park M, Mandal A, Mandal S, Wolff E. A new non-radioactive deoxyhypusine synthase assay adaptable to high throughput screening. Amino Acids. 2017;49:1793-1804 pubmed publisher
    Deoxyhypusine synthase (DHS) catalyzes the post-translational modification of eukaryotic translation factor 5A (eIF5A) by the polyamine, spermidine, that converts one specific lysine residue to deoxyhypusine [N ε -4-..
  37. Ilari A, Fiorillo A, Baiocco P, Poser E, Angiulli G, Colotti G. Targeting polyamine metabolism for finding new drugs against leishmaniasis: a review. Mini Rev Med Chem. 2015;15:243-52 pubmed
    ..Hypusination is a posttranslational modification occurring exclusively in the eukaryotic initiation factor 5A (eIF5A), which has an important role in avoiding the ribosome stalling during the biosynthesis of ..
  38. Pereira K, Tamborlin L, Meneguello L, de Proença A, Almeida I, Lourenço R, et al. Alternative Start Codon Connects eIF5A to Mitochondria. J Cell Physiol. 2016;231:2682-9 pubmed publisher
    ..In humans, several EIF5A1 transcript variants encode the canonical eIF5A1 isoform B, whereas the hitherto uncharacterized variant A is ..
  39. Olsen M, Cressey T, Mühlberger E, Connor J. Differential Mechanisms for the Involvement of Polyamines and Hypusinated eIF5A in Ebola Virus Gene Expression. J Virol. 2018;92: pubmed publisher
    Polyamines and hypusinated eIF5A have been implicated in the replication of diverse viruses; however, defining their roles in supporting virus replication is still under investigation...
  40. Sheehan G, Bergsson G, McElvaney N, Reeves E, Kavanagh K. The Human Cathelicidin Antimicrobial Peptide LL-37 Promotes the Growth of the Pulmonary Pathogen Aspergillus fumigatus. Infect Immun. 2018;86: pubmed publisher
    ..7-fold increased]). These results indicate that LL-37 stimulates A. fumigatus growth and that this stimulation can result in increased fungal growth and secretion of toxins in the lungs of CF patients. ..
  41. Wang X, Ma X, Huang L, Zhang X. Identification of the valid reference genes for quantitative RT-PCR in annual ryegrass (Lolium multiflorum) under salt stress. Molecules. 2015;20:4833-47 pubmed publisher
    ..We should choose optimal reference genes in specific tissues instead of the most stable one selected from different conditions and tissues. ..
  42. Chen H, Adam Arsovski A, Yu K, Wang A. Deep sequencing leads to the identification of eukaryotic translation initiation factor 5A as a key element in Rsv1-mediated lethal systemic hypersensitive response to Soybean mosaic virus infection in soybean. Mol Plant Pathol. 2017;18:391-404 pubmed publisher
    ..Knock-down of one of the identified DEGs, the eukaryotic translation initiation factor 5A (eIF5A), diminished the LSHR and enhanced viral accumulation, suggesting the essential role of eIF5A in the G7-induced, ..
  43. Melis N, Rubera I, Cougnon M, Giraud S, Mograbi B, Belaid A, et al. Targeting eIF5A Hypusination Prevents Anoxic Cell Death through Mitochondrial Silencing and Improves Kidney Transplant Outcome. J Am Soc Nephrol. 2017;28:811-822 pubmed publisher
    The eukaryotic initiation factor 5A (eIF5A), which is highly conserved throughout evolution, has the unique characteristic of post-translational activation through hypusination...
  44. Lee S, Park J, Kaevel J, Sramkova M, Weigert R, Park M. The effect of hypusine modification on the intracellular localization of eIF5A. Biochem Biophys Res Commun. 2009;383:497-502 pubmed publisher
    Eukaryotic translation initiation factor 5A (eIF5A) is a highly conserved protein essential for eukaryotic cell proliferation and is the only protein containing hypusine, [N(epsilon)-(4-amino-2-hydroxybutyl)lysine]...
  45. Ishfaq M, Maeta K, Maeda S, Natsume T, Ito A, Yoshida M. Acetylation regulates subcellular localization of eukaryotic translation initiation factor 5A (eIF5A). FEBS Lett. 2012;586:3236-41 pubmed publisher
    Eukaryotic translation initiation factor 5A (eIF5A) is a protein subject to hypusination, which is essential for its function. eIF5A is also acetylated, but the role of that modification is unknown...
  46. Seko Y, Fujimura T, Yao T, Taka H, Mineki R, Okumura K, et al. Secreted tyrosine sulfated-eIF5A mediates oxidative stress-induced apoptosis. Sci Rep. 2015;5:13737 pubmed publisher
    ..Here, we found that eIF5A undergoes tyrosine sulfation in the trans-Golgi and is rapidly secreted from cardiac myocytes in response to ..
  47. Facchiano A, Stiuso P, Chiusano M, Caraglia M, Giuberti G, Marra M, et al. Homology modelling of the human eukaryotic initiation factor 5A (eIF-5A). Protein Eng. 2001;14:881-90 pubmed
    ..Last, the model was deposited in the Protein Data Bank to be accessible for studies on the structure-function relationships of the human eIF-5A. ..
  48. Miyake T, Pradeep S, Wu S, Rupaimoole R, Zand B, Wen Y, et al. XPO1/CRM1 Inhibition Causes Antitumor Effects by Mitochondrial Accumulation of eIF5A. Clin Cancer Res. 2015;21:3286-97 pubmed publisher
    ..XPO1 inhibitor promoted the accumulation of eIF5A in mitochondria, leading to cancer cell death...
  49. Shin B, Katoh T, Gutierrez E, Kim J, Suga H, Dever T. Amino acid substrates impose polyamine, eIF5A, or hypusine requirement for peptide synthesis. Nucleic Acids Res. 2017;45:8392-8402 pubmed publisher
    ..Previous studies have shown that the translation factor eIF5A and its bacterial ortholog EF-P bind in the E site of the ribosome where they contact the peptidyl-tRNA in the P ..
  50. Hyvönen M, Keinänen T, Khomutov M, Simonian A, Vepsäläinen J, Park J, et al. Effects of novel C-methylated spermidine analogs on cell growth via hypusination of eukaryotic translation initiation factor 5A. Amino Acids. 2012;42:685-95 pubmed publisher
    ..function of spermidine in cell growth is its role as a butylamine donor for hypusine synthesis in the eukaryotic initiation factor 5A (eIF5A)...
  51. Dever T, Green R. The elongation, termination, and recycling phases of translation in eukaryotes. Cold Spring Harb Perspect Biol. 2012;4:a013706 pubmed publisher
    ..unique aspects of eukaryotic translation elongation including eEF1 recycling, eEF2 modification, and eEF3 and eIF5A function...
  52. Mittal N, Morada M, Tripathi P, Gowri V, Mandal S, Quirch A, et al. Cryptosporidium parvum has an active hypusine biosynthesis pathway. Mol Biochem Parasitol. 2014;195:14-22 pubmed publisher
    ..No fully effective treatment is available. Hypusination of eIF5A is an important post-translational modification essential for cell proliferation...
  53. Malyutin A, Musalgaonkar S, Patchett S, Frank J, Johnson A. Nmd3 is a structural mimic of eIF5A, and activates the cpGTPase Lsg1 during 60S ribosome biogenesis. EMBO J. 2017;36:854-868 pubmed publisher
    ..We find that a central domain of Nmd3 mimics the translation elongation factor eIF5A, inserting into the E site of the ribosome and pulling the L1 stalk into a closed position...
  54. Afanador G, Tomchick D, Phillips M. Trypanosomatid Deoxyhypusine Synthase Activity Is Dependent on Shared Active-Site Complementation between Pseudoenzyme Paralogs. Structure. 2018;26:1499-1512.e5 pubmed publisher
    ..The polyamine spermidine is essential for post-translational hypusine modification of eukaryotic initiation factor 5A (eIF5A), which is catalyzed by deoxyhypusine synthase (TbDHS)...
  55. Gulappa T, Menon B, Menon K. Hypusination of eukaryotic initiation factor 5A via cAMP-PKA-ERK1/2 pathway is required for ligand-induced downregulation of LH receptor mRNA expression in the ovary. Mol Cell Endocrinol. 2015;413:90-5 pubmed publisher
    ..b>Eukaryotic initiation factor 5A (EIF5A), identified as an LRBP-interacting protein plays a crucial role in LHR mRNA expression...
  56. Yao T, Fujimura T, Murayama K, Seko Y. Plasma levels of oxidative stress-responsive apoptosis inducing protein (ORAIP) in rats subjected to physicochemical oxidative stresses. Biosci Rep. 2016;36: pubmed publisher
    ..We named this novel post-translationally modified secreted form of eukaryotic translation initiation factor 5A (eIF5A) as oxidative stress-responsive apoptosis inducing protein (ORAIP)...
  57. Costa Neto C, Parreiras E Silva L, Ruller R, Oliveira E, Miranda A, Oliveira L, et al. Molecular modeling of the human eukaryotic translation initiation factor 5A (eIF5A) based on spectroscopic and computational analyses. Biochem Biophys Res Commun. 2006;347:634-40 pubmed
    The eukaryotic translation initiation factor 5A (eIF5A) is a protein ubiquitously present in archaea and eukarya, which undergoes a unique two-step post-translational modification called hypusination...
  58. Shang F, Xia Q, Liu W, Xia L, Qian B, You L, et al. miR-434-3p and DNA hypomethylation co-regulate eIF5A1 to increase AChRs and to improve plasticity in SCT rat skeletal muscle. Sci Rep. 2016;6:22884 pubmed publisher
    ..with SCT; these increases were reduced following the injection of a eukaryotic translation initiation factor 5A1 (eIF5A1) shRNA lentivirus into the hindlimb muscle...
  59. Lee S, Park J, Folk J, Deck J, Pegg A, Sokabe M, et al. Inactivation of eukaryotic initiation factor 5A (eIF5A) by specific acetylation of its hypusine residue by spermidine/spermine acetyltransferase 1 (SSAT1). Biochem J. 2011;433:205-13 pubmed publisher
    b>eIF5A (eukaryotic translation initiation factor 5A) is the only cellular protein containing hypusine [Nϵ-(4-amino-2-hydroxybutyl)lysine]...
  60. Quintas Granados L, Carvajal Gamez B, Villalpando J, Ortega Lopez J, Arroyo R, Azuara Liceaga E, et al. Bifunctional activity of deoxyhypusine synthase/hydroxylase from Trichomonas vaginalis. Biochimie. 2016;123:37-51 pubmed publisher
    ..In summary, our results indicate that T. vaginalis contains an active TvDHS capable of modifying the precursor TveIF-5A protein, which subsequently exhibits RNA binding activity. ..
  61. Shang Y, Zhao X, Tian B, Wang Y, Ren F, Jia B, et al. CHIP/Stub1 interacts with eIF5A and mediates its degradation. Cell Signal. 2014;26:1098-104 pubmed publisher
    b>eIF5A, containing the unusual amino acid hypusine, is a highly conserved protein essential for the proliferation of eukaryotic cells...
  62. Park M, Nishimura K, Zanelli C, Valentini S. Functional significance of eIF5A and its hypusine modification in eukaryotes. Amino Acids. 2010;38:491-500 pubmed publisher
    ..modification that occurs in only one cellular protein, eukaryotic translation initiation factor 5A (eIF5A, eIF-5A)...
  63. Lubas M, Harder L, Kumsta C, Tiessen I, Hansen M, Andersen J, et al. eIF5A is required for autophagy by mediating ATG3 translation. EMBO Rep. 2018;19: pubmed publisher
    ..Here we describe a role for the conserved eukaryotic translation initiation factor 5A (eIF5A) in autophagy...
  64. Duarte M, Lage D, Martins V, Costa L, Lage L, Carvalho A, et al. A vaccine combining two Leishmania braziliensis proteins offers heterologous protection against Leishmania infantum infection. Mol Immunol. 2016;76:70-9 pubmed publisher
    In the present study, two Leishmania braziliensis proteins, one hypothetical and the eukaryotic initiation factor 5a (EiF5a), were cloned and used as a polyproteins vaccine for the heterologous protection of BALB/c mice against infantum ..
  65. Koettnitz K, Kappel B, Baumruker T, Hauber J, Bevec D. The genomic structure encoding human initiation factor eIF-5A. Gene. 1994;144:249-52 pubmed
    A genomic clone encoding the human eukaryotic initiation factor 5A (eIF-5A) was isolated and its entire nucleotide sequence was determined. The whole eIF-5A coding region is not interrupted by introns...
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    ..small interference RNA (siRNA) to knock down the expression of eukaryotic translation initiation factor 5A (eIF5A) in corneal epithelial cells...
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    ..The expression levels of IMPDH2, CK20, MAP3K8 and EIF5A were strongly up-regulated in CRC tissues compared with normal colorectal tissues (p < 0.05)...
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    ..Two of these proteins, are eukaryotic translation initiation factor 5A1 (eIF5A1) that is involved in cell survival and proliferation, and Rho GDP dissociation inhibitor alpha (RhoGDIα), a ..
  69. Zhang J, Li X, Liu X, Tian F, Zeng W, Xi X, et al. EIF5A1 promotes epithelial ovarian cancer proliferation and progression. Biomed Pharmacother. 2018;100:168-175 pubmed publisher
    ..We found abundant eukaryotic translation initiation factor 5A1 (EIF5A1) in 54 EOC tissues, and high EIF5A1 levels predicted poor survival...
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    ..is involved in the critical post-translational modification of the eukaryotic translation initiation factor 5A (eIF5A)...
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    Hypusine modification of the eukaryotic initiation factor 5A (eIF-5A) is emerging as a crucial regulator in cancer, infections, and inflammation...
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    ..5'-cDNA ends (5'-RACE) as a detection probe, we have isolated a new genomic clone encoding the human eukaryotic initiation factor 5A (eIF-5A)...
  73. Xu G, Shi X, Sun L, Zhou Q, Zheng D, Shi H, et al. Down-regulation of eIF5A-2 prevents epithelial-mesenchymal transition in non-small-cell lung cancer cells. J Zhejiang Univ Sci B. 2013;14:460-7 pubmed publisher
    ..Recent studies suggested that eukaryotic initiation factor 5A-2 (eIF5A-2) might serve as an adverse prognostic marker of survival...
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    The enzyme deoxyhypusine hydroxylase (DOHH) catalyzes the activation of eukaryotic translation initiation factor (eIF5A), a protein essential for cell growth...
  75. Moghanibashi M, Rastgar Jazii F, Soheili Z, Zare M, Karkhane A, Parivar K, et al. Esophageal cancer alters the expression of nuclear pore complex binding protein Hsc70 and eIF5A-1. Funct Integr Genomics. 2013;13:253-60 pubmed publisher
    ..overexpression of Hsc70 and downregulation of subunit alpha type-3 of proteasome, calpain small subunit 1, and eIF5A-1...
  76. Takeuchi K, Nakamura K, Fujimoto M, Kaino S, Kondoh S, Okita K. Heat stress-induced loss of eukaryotic initiation factor 5A (eIF-5A) in a human pancreatic cancer cell line, MIA PaCa-2, analyzed by two-dimensional gel electrophoresis. Electrophoresis. 2002;23:662-9 pubmed
    ..These results suggest that loss of the active form of eIF-5A is an important factor in the irreversible process of heat stress-induced death of MIA PaCa-2 cells. ..
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    ..Despite comprehensive studies addressing PAs, a unifying concept to interpret their molecular role is missing. The precise biochemical function of polyamines is thus one of the remaining mysteries of molecular cell biology. ..
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    ..These findings would support a model whereby eIF-5A stimulates peptide bond formation on polyproline-stalled ribosomes by stabilizing and orienting the CCA-end of the P-tRNA, rather than by directly contributing to the catalysis. ..
  79. Barbosa N, Boldrin P, Rossi D, Yamamoto P, Watanabe T, Serrão V, et al. Mapping surface residues of eIF5A that are important for binding to the ribosome using alanine scanning mutagenesis. Amino Acids. 2016;48:2363-74 pubmed publisher
    The translation elongation factor eIF5A is conserved through evolution and is necessary to rescue the ribosome during translation elongation of polyproline-containing proteins...
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    ..Spermidine is essential for viability and acts as the precursor of hypusine, a post-translational addition to eIF5A allowing the translation of mRNAs encoding proteins containing polyproline tracts...
  81. Fujimura K, Wang H, Watson F, Klemke R. KRAS Oncoprotein Expression Is Regulated by a Self-Governing eIF5A-PEAK1 Feed-Forward Regulatory Loop. Cancer Res. 2018;78:1444-1456 pubmed publisher
    ..feed-forward regulatory loop that utilizes a unique hypusinated isoform of the translation elongation factor eIF5A and the tyrosine kinase PEAK1...
  82. Park M, Wolff E, Smit McBride Z, Hershey J, Folk J. Comparison of the activities of variant forms of eIF-4D. The requirement for hypusine or deoxyhypusine. J Biol Chem. 1991;266:7988-94 pubmed
    ..These findings emphasize the importance of hypusine or deoxyhypusine for the biological activity of eIF-4D and demonstrate the influence of both the length and chemical nature of its amino alkyl side chain...
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  84. Singh U, Li Q, Cerione R. Identification of the eukaryotic initiation factor 5A as a retinoic acid-stimulated cellular binding partner for tissue transglutaminase II. J Biol Chem. 1998;273:1946-50 pubmed
    ..The interaction between TGase and eIF-5A and its sensitivity to the nucleotide-occupied state of the TGase provides a potentially interesting connection between RA signaling and protein synthesis and/or RNA trafficking activities. ..
  85. Tariq M, Ito A, Ishfaq M, Bradshaw E, Yoshida M. Eukaryotic translation initiation factor 5A (eIF5A) is essential for HIF-1α activation in hypoxia. Biochem Biophys Res Commun. 2016;470:417-424 pubmed publisher
    The eukaryotic initiation factor 5A (eIF5A) is an essential protein involved in translation elongation and cell proliferation. eIF5A undergoes several post-translational modifications including hypusination and acetylation...
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    b>eIF5A is the only protein known to contain the essential and unique amino acid residue hypusine...
  87. Restelli L, Marques A, Savoini G, Invernizzi G, Carisetti M, Lecchi C, et al. Saturated or unsaturated fat supplemented maternal diets influence omental adipose tissue proteome of suckling goat-kids. Res Vet Sci. 2017;: pubmed publisher
    ..Four proteins, namely PMSC1, PPIB, TUB5×2 and EIF5A1, were be less abundant in both ST- and FO- kids...
  88. Steinkasserer A, Jones T, Sheer D, Koettnitz K, Hauber J, Bevec D. The eukaryotic cofactor for the human immunodeficiency virus type 1 (HIV-1) rev protein, eIF-5A, maps to chromosome 17p12-p13: three eIF-5A pseudogenes map to 10q23.3, 17q25, and 19q13.2. Genomics. 1995;25:749-52 pubmed
    The eukaryotic initiation factor 5A (eIF-5A) has been identified as an essential cofactor for the HIV-1 transactivator protein Rev...
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    b>Eukaryotic initiation factor 5A (eIF-5A) is the only protein in nature that contains hypusine, an unusual amino acid formed post-translationally by deoxyhypusine synthase and deoxyhypusine hydroxylase...
  90. Hoque M, Hanauske Abel H, Palumbo P, Saxena D, D Alliessi Gandolfi D, Park M, et al. Inhibition of HIV-1 gene expression by Ciclopirox and Deferiprone, drugs that prevent hypusination of eukaryotic initiation factor 5A. Retrovirology. 2009;6:90 pubmed publisher
    Eukaryotic translation initiation factor eIF5A has been implicated in HIV-1 replication...
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    ..Proteins were identified as Cyclophilin A, EIF5A-1 isoform B, Rho GDI 1 isoform a, VDAC1, OTUB1 and α-enolase isoform 1 (ENO1) through LC-MS/MS...
  92. Cáceres C, Angulo J, Contreras N, Pino K, Vera Otarola J, López Lastra M. Targeting deoxyhypusine hydroxylase activity impairs cap-independent translation initiation driven by the 5'untranslated region of the HIV-1, HTLV-1, and MMTV mRNAs. Antiviral Res. 2016;134:192-206 pubmed publisher
    Replication of the human immunodeficiency virus type 1 (HIV-1) is dependent on eIF5A hypusination...